Point-of-care detection system for aquatic microorganisms

A portable isothermal heater system with RPA and NALFIA allows for rapid and accurate detection of aquatic microorganisms, addressing the limitations of existing methods by providing accessible and user-friendly solutions for non-scientists.

WO2025250270A1PCT designated stage Publication Date: 2025-12-04GIANNASCA NICHOLAS
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Patent Information

Application Number
PCT/US2025/025197
Authority / Receiving Office
WO · WO
Patent Type
Applications
Current Assignee / Owner
Priority Date
2024-05-31
Filing Date
2025-04-17
Publication Date
2025-12-04

AI Technical Summary

Technical Problem

Current methods for detecting aquatic microorganisms, particularly in non-laboratory settings, are time-consuming, require specialized equipment, and are not accessible to non-scientists, hindering rapid intervention in aquatic ecosystems.

Method used

A portable, hand-held isothermal single-sample heater system for nucleic acid amplification and detection, using a lysis agent, Recombinase Polymerase Amplification (RPA), and nucleic acid lateral flow immunoassays (NALFIA) for rapid, user-friendly detection of aquatic microorganisms.

Benefits of technology

Enables rapid, accurate, and accessible detection of pathogenic and beneficial aquatic microorganisms, facilitating timely intervention and management in aquatic environments.

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Abstract

A system and method are provided for detecting aquatic microorganisms. The system comprises a nucleic acid extraction step for extracting nucleic acid from aquatic samples, a nucleic acid amplification step for amplifying the extracted genomic material, and a nucleic acid detection step for detecting the presence of amplified nucleic acids, indicating the presence of microorganisms. The method and system are applicable to both marine and freshwater environments and can be used for point-of-care detection of pathogenic and / or beneficial aquatic microorganisms. A nucleic acid amplification component comprising a hand-held isothermal heater to amplify the extracted nucleic acid, and a nucleic acid detection component configured to detect amplified nucleic acid. The hand-held isothermal heater includes a main body, a sample port configured, to receive a sample tube, a viewing screen, a heat block, and an electronics compartment. The system enables rapid and efficient detection of pathogenic and beneficial aquatic microorganisms.
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Citation Information

Patent Citations

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