Illumination system, medical fluorescence imaging system, and system

Abstract

The invention relates to an illumination device (10) for a medical fluorescence imaging system, comprising: an optical interface (20) for output coupling illumination light to a medical imaging system; and an illumination unit (30) configured to supply illumination light to the optical interface, comprising: a first luminous element (40) configured to generate narrowband first illumination light with a first illumination spectrum for exciting at least two fluorescent dyes with different excitation spectra from a first spectral group, a second luminous element (50) configured to generate narrowband second illumination light with a second illumination spectrum for exciting at least two fluorescent dyes with different excitation spectra from a second spectral group, a third luminous element (60) configured to generate narrowband third illumination light with a third illumination spectrum for exciting at least two fluorescent dyes with different excitation spectra from a third spectral group, wherein the illumination unit (30) is operable in three different illumination modes; and wherein the first illumination spectrum, the second illumination spectrum and the third illumination spectrum are spectrally disjoint. The invention also relates to a medical fluorescence imaging system and a system.

Description

Lighting system, medical fluorescence imaging system and system The invention relates to a lighting device for a medical device. Fluorescence imaging system, a medical fluorescence imaging system and a system with a lighting device or a medical device Fluorescence imaging system and fluorescent dyes. Fluorescence imaging systems are used particularly in surgeries involving a An endoscope and / or an exoscope is used for this purpose. For this, a patient is examined before the procedure. The operation involved administering a fluorescent dye that is found in a known Tissue layer, for example a tumor, a nerve or a lymphatic vessel, deposited. Subsequently, during the operation, the fluorescent dye is applied with Light illuminated that falls within a wavelength range of the excitation spectrum of the fluorescent dye is located, and thus excited in such a way that the fluorescent dye Light is emitted across its emission spectrum. The emitted light is then detected and can thus be used by an operator in a recording of a The surgical area will be marked or color-coded. In particular, Fluorescence imaging systems are used in minimally invasive applications. This is particularly useful for marking different types of objects. Different fluorescent dyes are used in different tissue layers. Previously known lighting systems for medical However, fluorescence imaging systems are only suitable for the optimal excitation of designed with a fluorescent dye with a specific excitation maximum. These lighting systems are often so heavily focused on just one Fluorescent dye adapted to match spectrally similar fluorescent dyes, which has a spectrally close excitation maximum to the fluorescent dye exhibit only suboptimal stimulation. Known lighting systems are sometimes designed to be excited by only one fluorescent dye, that even a shift of an excitation maximum of 20 nm in the direction of short-wavelength light from a spectrally similar fluorescent dye sufficient to no longer sufficiently excite this fluorescent dye can. Also, previously known medical fluorescence imaging systems for the Designed for optimal detection of only one fluorescent dye and can They barely detect spectrally similar fluorescent dyes. Because of this... known lighting systems and medical Fluorescence imaging systems for the optimal excitation and detection of only If a fluorescent dye is used, a separate [solution / design] must be provided for each fluorescent dye. different lighting systems and / or different medical equipment Fluorescence imaging systems are used. The acquisition of the different lighting systems and / or medical However, fluorescence imaging systems cause very high surgical costs. Therefore, one of the tasks of the invention is to create a versatile and cost-efficient device. Illumination system for a medical fluorescence imaging system to provide. Furthermore, the invention aims to provide a versatile and cost-efficient solution. to provide a medical fluorescence imaging system. These tasks are accomplished according to the invention by a lighting device and a medical fluorescence imaging system as described in the claims are defined and described herein. The lighting device includes an optical interface for extraction. from illumination light to a medical imaging system and a Lighting unit designed to direct illumination light to the optical To provide an interface. The lighting unit includes a first lighting element, which is designed to provide narrowband first illumination with a first Illumination spectrum for the excitation of at least two fluorescent dyes with different excitation spectra from a first spectral group to generate. Furthermore, the lighting unit includes a second lighting element, which is designed to provide narrowband second lighting with a second Illumination spectrum for the excitation of at least two fluorescent dyes with different excitation spectra from a second spectral group generate. Furthermore, the lighting unit includes a third lighting element, which is designed to provide narrowband third lighting with a third Illumination spectrum for the excitation of at least two fluorescent dyes with different excitation spectra from a third spectral group generate. Furthermore, the lighting unit is available in three different versions. Lighting modes can be operated, with the first lighting spectrum, the second The illumination spectrum and the third illumination spectrum are spectrally disjoint. This results in versatile applicability. Furthermore, a high degree of Cost efficiency is achieved. In particular, the invention allows for cost efficiency. Lighting device provides an optimized representation of a group or a Clusters of fluorescent dyes can be achieved with just one overall system. By specifically clustering and / or grouping existing dyes Optimization can be achieved by applying a lighting device to clusters or... The focus is now on groups of dyes, rather than individual dyes. Through the Use of three lighting elements with three different Illumination spectra and the grouping of dyes into three Different spectral groups lead to broad coverage of the relevant area. spectral range in which medically safe substances can be used Fluorescent dyes are present. At the same time, a low degree of complexity is observed. This is achieved because not every dye requires a specifically chosen individual approach. One lighting strategy needs to be chosen, but three different ones. Illumination spectra are sufficient. Through targeted selection of three Different lighting spectra can therefore be a good compromise between spectral overlap on the one hand and low effort in the On the other hand, the design and manufacture of a lighting device achieves become. The lighting device includes, in particular, an optical interface and A lighting unit for emitting illuminating light. The optical The interface can, in particular, be an optic for extracting the illumination light. include. The first, second and / or third lighting element can in particular be an LED. and / or a laser light source, for example a laser diode, and / or a laser Array and / or a cold light source with halogen lamps and / or with xenon light source include, for example, those using fiber optics to connect to an endoscope and / or a Another optical device is coupled in. The lighting elements each produce narrowband or monochromatic light. Illumination light. Monochromatic illumination light is particularly Monochromatic light. For example, monochromatic lighting. electromagnetic radiation in a precisely defined wavelength range and / or at a specific wavelength. A spectral width of the Illumination spectrum of the first luminaire element and / or the second The lighting element and / or the third lighting element can, for example, have a maximum of 50 nm, at most 25 nm, at most 20 nm or even at most 15 nm. The aforementioned spectral width can be expressed as a half-width of the Refer to intensity peaks of the illumination spectrum. Furthermore, the illumination light of a lighting element can be, in particular, such as… It should be chosen that the illumination spectrum of the illumination light has several It stimulates fluorescent dyes of the spectral group. At least, that stimulates it. The illumination spectrum shows two fluorescent dyes of the spectral group. Preferably, the illumination spectrum of the illumination light is optimized in such a way that that 2, 3, 4, 5, 6, 7 or more different fluorescent dyes of a Spectral group sufficient with the lighting element of a luminaire be stimulated and / or optimized. The first spectral group can include, for example, fluorescent dyes. Demeclocycline-1, Verteporfin-1, Aftobetin-1, MB102-1, Fluorescein-4, AF488-1, be assigned to FITC and / or Bodipy-FL-1. Preferably, the first The fluorescent dyes Bodipy-FL-1 and FITC were assigned to the spectral group. The first spectral group includes, for example, fluorescent dyes that emit light into emit in a visible blue wavelength range (420 – 490 nm). The second spectral group can include, for example, fluorescent dyes. BM104-1, Cy5.5-4, Cy5-4, Cy5 and / or Cy5.5. Preferably, they are the second spectral group includes the fluorescent dyes Cy5 and / or Cy5.5 assigned. The second spectral group includes, for example, Fluorescent dyes that emit light in a visible red wavelength range (650 nm) emit at wavelengths of 780 nm. The third spectral group can be represented, for example, by the fluorescent dyes ZW800- 1, Cy7-2, IRDye800-11, ICG-3, S0456-1, Cypate-1, IRDye800 CW and / or ICG be assigned to the third spectral group. Preferably, the following are assigned to the third spectral group: assigned fluorescent dyes IRDye800 CW and / or ICG. The third The spectral group includes, for example, fluorescent dyes that emit light in a Emit in the NIR wavelength range (800 – 2500 nm). One of the three different lighting modes includes in particular, that light from the first lighting element and / or the second lighting element and / or the third lighting element is delivered. The three different lighting modes can provide a first impression. Lighting mode includes the light from the first lighting element. is delivered. In some versions, the first lighting mode is the The second lighting element and / or the third lighting element is deactivated. The three different lighting modes can be used alternatively or additionally to... include a second lighting mode in which the light from the second The lighting element is emitted. In some versions, it is in the second Lighting mode: the first lighting element and / or the third Lighting element deactivated. The three different lighting modes may alternatively or additionally include a third lighting mode, in the light emitted by the third lighting element. In some In the third lighting mode, the first lighting element is the variant. and / or the second lighting element is deactivated. Spectrally disjoint means, in particular, that the first illumination spectrum, which second illumination spectrum and the third illumination spectrum none have overlapping wavelength ranges. Preferably the maximum wavelength range of the first illumination spectrum of the minimum wavelength range of the second illumination spectrum spaced Furthermore, the maximum wavelength range of the second can preferably be illumination spectrum from the minimum wavelength range of the third The illumination spectrum should be spaced apart. These details may vary. for example, referring to the intensity of one of the lighting elements in the The illumination spectrum of any other lighting element is reduced to a maximum of 1%. at most 0.1% or at most 0.01% of its maximum intensity. In In other words, the intensity in the area in question can effectively be zero. The inventors recognized that three known lighting elements could be used. Common fluorescent dyes can be effectively excited. This is This is particularly the case because the known or common ones Fluorescent dyes can be classified into three spectral groups, for which then Each one has its own dedicated lighting element. The inventors have also recognized that the illumination spectrum of the The illumination of the lighting element, or its maximum, is optimized in this way. It can be chosen that for several fluorescent dyes of a spectral group Sufficient and / or optimized stimulation is achieved. To determine the The illumination spectrum can, in particular, be the excitation spectrum of one of the Fluorescent dyes are multiplied by the illumination spectrum. This The product is referred to as a weighted illumination spectrum. The integral of the The weighted illumination spectrum yields the respective sensitivity of the Fluorescent dye as a function of the illumination spectrum. The sensitivity in The dependence of the illumination spectrum can be observed for the fluorescent dyes of the The spectral group can be calculated. By determining the sensitivity of the Fluorescent dyes of the spectral group can be used to determine the illumination spectrum. will be optimized for the fluorescent dyes of the spectral group. become. Furthermore, to determine the illumination spectrum, a Observational effectiveness can be determined. For this purpose, a spectral analysis can first be performed. Overlap of an excitation spectrum of one of the fluorescent dyes with a The system's observation spectrum must be determined. The observation effectiveness can be determined by the integral of the spectral overlap. Through the Sensitivity and observation effectiveness of the individual fluorescent dyes For a spectral group, the illumination spectrum for which the Fluorescent dyes of the spectral group are optimally excited. Furthermore, the following additional methods can be used to determine the illumination spectrum Parameters to be considered: an optimum of excitation and detection of the fluorescent dye, a quantum yield of the fluorescent dye, a Concentration of the fluorescent dye in a tissue layer, a distribution of the Fluorescent dye in the tissue layer (signal-to-noise ratio) and / or Properties of the tissue layer and / or of tissue that forms the tissue layer surrounding the area in which the fluorescent dye is incorporated. These properties Examples include pH value, autofluorescence and / or color contrast. be. Preferably, in each of the three different lighting modes, only one of the lighting elements is activated to clearly distinguish between the Fluorescent dyes of the different spectral groups differ in that can. However, it is also possible that in certain lighting modes two are possible. or all three light-emitting elements are activated to emit multiple fluorescent dyes to be able to stimulate at the same time. To achieve optimized excitation of the fluorescent dyes of a spectral group to ensure and / or to ensure that the emitted light of the Fluorescent dyes can be detected in an optimized way, which can be the first step. Illumination spectrum, in particular an intensity maximum at a first wavelength, the second illumination spectrum in particular a Intensity maximum at a second wavelength and the third Illumination spectrum, in particular an intensity maximum at a third exhibit wavelength. The first wavelength can be found particularly in a range of 445 nm to 500 nm. The second wavelength can be found particularly in a range from 630 nm to 670. In addition, the third wavelength can be found particularly in a range of The range is between 755 nm and 795 nm. Selecting these ranges ensures a particularly balanced overlap with the excitation spectra of common dyes. Although the three peak wavelengths of the three light elements used are clearly spaced apart from each other and the illumination spectra disjoint and also are spaced apart, in this case a large number of relevant Dyes are selectively stimulated by activating one of the light elements at a time. becomes. Furthermore, the first wavelength can preferably be in a range from 445 nm to 485 nm. The first wavelength can be particularly favorably located in a range from 455 nm to 475 nm. These ranges have proven to be particularly suitable. for the first luminous element if a is present in the first spectral group The focus will be on the dye fluorescein. Dyes of the first group can then be stimulated by means of the first lighting element, whereby especially for fluorescein, there is good spectral overlap, and therefore a High quantum efficiency can be achieved upon its excitation. The first spectral group This can be directed at dyes that are in the visible range, especially in the blue, green and yellow spectral range, they are excitable. Furthermore, the first wavelength can preferably be in a range from 460 nm to 500 nm. The first wavelength can be particularly preferably located in a range of 500 nm. from 470 nm to 500 nm. These ranges have proven to be particularly suitable. for the first luminous element if a is present in the first spectral group The focus will be on the dye MB-102. Dyes of the first group can then be stimulated by means of the first lighting element, whereby especially for MB-102 there is a good spectral overlap and therefore a high Quantum efficiency can be achieved upon its excitation. The first spectral group can the focus should be on such dyes that are in the visible range, in particular in the blue, green and yellow spectral range, are excitable. Preferably, the second wavelength can be in the range of 640 nm to 660 nm. lie in this area. Dyes of the second spectral group are particularly pronounced. This can result in Selection of the second wavelength for dyes excitable in the red spectral range effectively stimulated by the second lighting element. Furthermore, the third wavelength can preferably be in a range from 765 nm to 785 nm. In this range, the emission spectra of the The relevant dyes of the third spectral group are particularly pronounced. This results in can be used when choosing the third wavelength for the near-infrared spectral range Excitable dyes are effectively stimulated by the third luminescent element. In addition, the lighting unit can include a white light element. Activating the white light element can activate the lighting unit It can be operated, in particular, in a white light mode. This makes it especially It is possible to realistically simulate the environment of fluorescent tissue layers. to represent overlay representations. Furthermore, this method can be used to create overlay representations for A user is created in which a white light image is combined with a fluorescent image. is superimposed. The lighting unit can be specifically designed to separate the white light mode and one of the three different lighting modes to switch, especially to flash. It can be repeatedly alternately in the White light mode and an image capture in one of the lighting modes. This can also happen multiple times per second, for example at least 10- times or at least 20 times or at least 30 times per second, so that Real-time images can be captured. Different cycles are possible. For example, several white light images can be taken one after the other. are taken before a fluorescence image is acquired, followed in turn by multiple white light images, etc. In other words, frame rates can be used for the White light mode and the lighting mode in question may differ. This allows, for example, a combined representation between the fluorescent tissue layers and the surrounding tissue layers The tissue layers can be represented in color. Furthermore, one of the above tasks is performed by a medical professional. Fluorescence imaging system solved, which uses an illumination device according to one of the preceding claims and comprises an imaging instrument. The imaging instrument includes an image acquisition sensor and a Observation filter unit. In particular, the observation filter unit includes a first observation filter, which includes an edge filter that is above a first edge wavelength is transparent and opaque below the first edge wavelength, to filter out the first illumination spectrum of the first lighting element. In addition, the observation filter unit can have a second observation filter. include, which is transparent above a second edge wavelength and below the second edge wavelength is opaque in order to transmit the second to filter out the illumination spectrum of the second light element. Furthermore, the The observation filter unit includes a third observation filter located above a third edge wavelength is transparent and below the third The edge wavelength is opaque to achieve the third illumination spectrum of the to filter out the third lighting element. Furthermore, in particular the first, second and third edge wavelength They may be of different sizes. This results in a different edge wavelength for the medical device. Fluorescence imaging system to the first, second and third The lighting spectrum is adjusted. This can be achieved in particular by adjusting the first [missing information]. The edge wavelength can be 10 to 20 nm larger than the first wavelength. Furthermore, in particular the second edge wavelength is 10 to 20 nm larger than the second wavelength. In addition, the third edge wavelength in particular can be 10 to 20 nm larger than the third wavelength. Furthermore, the first, second and / or third observation filter can be a bandpass filter. or a notch filter. By using a bandpass filter or notch filter Filters make it possible to filter only the respective first, second, or third to filter out the illumination spectrum of the illumination light. For this purpose, the first, second and / or third observation filters provide a narrow light-transmitting spectrum, especially between 10 and 50 nm and / or between 10 and 25 nm and / or between 15 and 20 nm. For example, the first one blocks The observation filter blocks precisely the first illumination spectrum. Furthermore, it blocks for example, the second observation filter is exactly the second one Illumination spectrum. Furthermore, the third observation filter blocks, for example, precisely the third illumination spectrum. In particular, the first one blocks observation filters precisely for the first wavelength and / or the second The present invention will now be described with reference to the accompanying figures. The drawing, the description, and the claims are described as an example. They contain numerous features in combination. The expert will recognize these features. It is also advisable to consider them individually and within the scope of the claims Use in combination. If more than one copy of a particular object exists, then only one copy may be available. one of them is indicated in the figures and description with a reference mark. provided. The description of this specimen can be applied accordingly to the others. Copies of the object are transferred. Are objects particularly by means of named using number words, such as first, second, third object, etc. These serve to name and / or classify objects. Accordingly, they can for example, a first object and a third object, but no second object This should be included. However, number words could also be used to additionally include a The number and / or sequence of objects can be derived. They show: Figure 1 Fluorescent dyes depending on their excitation and Emission wavelength, which in an exemplary embodiment a first spectral group, a second spectral group and a can be assigned to the third spectral group; Figure 2 Excitation and emission wavelengths of various Fluorescent dyes as a function of a normalized intensity and a wavelength; Figure 3 an exemplary embodiment of an invention medical fluorescence imaging system. Figure 1 shows fluorescent dyes depending on their excitation and Emission wavelength, which in an exemplary embodiment of a first spectral group 1, a second spectral group 2 and a third spectral group 3 can be assigned to the first spectral group 1, for example, the Fluorescent dyes Demeclocycline-1, Verteporfin-1, Aftobetin-1, MB102-1, assigned to Fluorescein-4 and / or AF488-1. The second spectral group 2. For example, the fluorescent dyes BM104-1, Cy5.5-4 and / or Cy5-4 can be used. be assigned to the third spectral group 3, for example, the Fluorescent dyes ZW800-1, Cy7-2, IRDye800-11, ICG-3, S0456-1 and / or assigned to Cypate-1. Figure 2 shows excitation and emission wavelengths of various Fluorescent dyes as a function of a normalized intensity and a Wavelength. Figure 2 shows the fluorescent dyes of a first spectral group 1', a second spectral group 2' and a third spectral group assigned to 3'. Examples of the first spectral group 1' are: Fluorescent dyes BodyFL and FITC, of ​​the second spectral group 2' Fluorescent dyes Cy5 and Cy5.5 and the third spectral group 3' the Fluorescent dyes IRDye800CW and ICG were assigned. The inventors recognized that the fluorescent dyes, which were the first Spectral group 1', the second spectral group 2' and the third spectral group 3' each assigned a lighting spectrum of Lighting unit of a light source sufficiently or optimally excited and / or the light emitted by the fluorescent dyes is sufficient or optimized can be recorded. The illumination spectrum is recorded in such a way that... chosen that the fluorescent dyes of the respective spectral groups 1', 2' and 3' They can be optimized, stimulated, and / or recorded. Three things are involved. The luminescent elements are sufficient to illuminate common fluorescent dyes, which are particularly common in the three spectral groups 1', 2' or 3 can be combined, to sufficiently stimulate them so that they emit enough light and the emitted Light can be recorded sufficiently. The fluorescent dyes of the first spectral group 1' can be described by a first Illumination spectrum of a first illumination light of a first luminous element They are optimally stimulated. The fluorescent dyes of the second spectral group 2' can be achieved through a second illumination spectrum of a second illumination light a second light-emitting element is optimally stimulated. The fluorescent dyes of the third spectral group 3' can be determined by a third illumination spectrum of a The third illumination light from a third lighting element is optimized. To achieve optimized excitation of the fluorescent dyes of a spectral group to ensure and / or to ensure that the emitted light of the The first study shows that fluorescent dyes can be optimally recorded. The illumination spectrum has an intensity maximum at a first wavelength of 45, which The second illumination spectrum shows an intensity maximum at a second wavelength. 55 and the third illumination spectrum has an intensity maximum at a third Wavelength 65. The first wavelength, 45, lies particularly in a range from 445 nm to 500 nm. nm. The second wavelength 55 lies particularly in a range from 630 nm to 670. In addition, the third wavelength 65 lies particularly in a range of 755. nm to 795 nm. Furthermore, the first wavelength 45 can preferably be in a The range is from 445 nm to 485 nm. The first one is particularly favored. Wavelength 45 in a range from 455 nm to 475 nm. Furthermore, the first Wavelength 45 preferably lies in a range of 460 nm to 500 nm. The first wavelength 45 is particularly favored in a range from 470 nm to 500 nm. Preferably, the second wavelength 55 can be in a range from 640 nm to 660 nm. nm. Furthermore, the third wavelength 65 can preferably be in a range of 765 nm to 785 nm. Figure 3 shows an exemplary embodiment of a system according to the invention. 200 with a medical fluorescence imaging system according to the invention 100. The fluorescence imaging system 100 includes an illumination device. 10 and an imaging instrument 110. The lighting device 10 comprises a lighting unit 30, an optical Interface 20 and optionally a white light element 70. The lighting unit 30 comprises a first lighting element 40, a second Lighting element 50 and a third lighting element 60. The first lighting element 40 is set up for this purpose, narrowband first lighting light with a first To generate an illumination spectrum. The first illumination spectrum is... for example, chosen such that at least two of the fluorescent dyes that are in Figure 1 of the first spectral group are sufficient and / or optimized be stimulated. The second lighting element 50 is designed for this purpose. narrowband second illumination light with a second illumination spectrum to generate. The second illumination spectrum is, for example, such as... chosen that at least two of the fluorescent dyes shown in Figure 1 of the second are assigned to spectral groups and are sufficiently and / or optimally stimulated. The third lighting element 60 is designed to provide a narrowband second To generate illumination light with a second illumination spectrum. This involves... The third illumination spectrum, for example, is chosen such that at least two the fluorescent dyes that are assigned to the third spectral group in Figure 1, be sufficiently and / or as strongly as possible stimulated. Preferably always only the first lighting element 40, the second lighting element 50 or the third Lighting element 60 of lighting unit 30 is activated simultaneously. Light from the white light luminaire element 70 and light from the lighting unit 30 will presented here by a purely exemplary beam combiner 80 combined. Subsequently, the light emerges from the optical interface 20. Lighting unit 10 off. It goes without saying that this is the Beam combiner 80 to combine several beam combiners or mirrors can act. For example, an arrangement of several crossed beam splitters can can be used. The representation in Figure 3 is therefore only schematic. understand. The crucial point is that the light from the different lighting elements (40) 50, 60 and in some embodiments of the white light illuminating element 70 is combinable and the lighting device 10 is illuminated via a defined light path can leave. Furthermore, preferably only the first lighting element is 40, the second Lighting element 50 or the third lighting element 60 of the lighting unit 30 or The white light element 70 is activated simultaneously. In particular, a distinction can be made between the white light element 70 and one of the light elements 40, 50 or 60 of the Lighting unit 30 switched quickly back and forth, especially flashing, This will happen. For example, even when the lighting unit is activated, it is always possible. Another lighting element (40, 50, or 60) may be activated alternately. For example The first lighting element can also be 40, the second lighting element 50, the third Lighting element 60 and the white light lighting element 70 are switched on one after the other. will be caught speeding or flashed. The light emanating from the optical interface 20 of the lighting device 10 exits, encounters an object 210 in which at least one fluorescent dye is present. The fluorescent dye is introduced. The light excites the fluorescent dye, causing it to emit light. Light. As shown in Figure 1, the emission occurs relative to the excitation. red-shifted. For example, several different objects can be placed in object 210. Fluorescent dyes may be incorporated. For example, object 210 human tissue and / or different tissue layers and / or different tissue types are involved. The fluorescent dyes are used in this process. especially used to isolate individual tissue layers and / or tissue types spectrally distinguishable from other tissue layers and / or tissue types make. The imaging instrument 110 includes, for example, an image acquisition sensor system. 120 and another image acquisition sensor 130, an optical interface 190, a beam splitter 180 and an observation filter unit 140. The beam splitter 180 The additional image acquisition sensors (130) are optional. for example, they are used for white light imaging. In particular, they can It may be useful if a white light mode is provided. The light from the lighting device 10 and the light emitted by at least An excited fluorescent dye is released and passes through the optical Interface 180 into the imaging instrument 110. That is, first light enters with a wavelength component that corresponds to the illumination spectrum of the illumination unit 10 corresponds to and a wavelength component that corresponds to the emission wavelength of the corresponding to the respective fluorescent dye, through the optical interface 180 in the imaging instrument 110. The optical interface 180 directs the light particularly into a The beam path is focused. The beam then meets a beam splitter. 180. The beam splitter 180 splits the beam path into two different paths. The beam paths are split. One of the two different beam paths hits... onto the observation filter unit 140 and then onto the Image acquisition sensor 120. The observation filter unit 140, for example, has a first observation filter 150, a second observation filter 160 and a third observation filter 170 through which different Observation modes can be implemented. The other of the two different ones The beam paths, especially without additional filtering, encounter the further Image capture sensor 130 to implement a white light observation mode. The additional image acquisition sensor 130 can in particular include an RGB chip. include. The observation filter unit 140 first filters the light with the Wavelength component that corresponds to the illumination spectrum of the illumination unit 10 corresponds to either the first observation filter 150, the second Observation filter 160, the third observation filter 170, or another one Observation filter removed. The first observation filter 150, for example, is designed to... Illumination spectrum of the first luminaire element 40 of the lighting unit 30 to filter out. The second observation filter 160 is used for this purpose, for example. set up, the lighting spectrum of the second lighting element 50 of the The illumination unit 30 is to filter out the light. The third observation filter 170 is... for example, designed to adjust the lighting spectrum of the third To filter out the light element 60 of the lighting unit 30. Accordingly The first observation filter 150 is used in particular when the first When the luminous element 40 is activated, the second observation filter 160 is used. the second lighting element 50 is activated and the third observation filter 170 used when the third lighting element 60 is activated. For example, the Observation filter unit 140 for switching through observation filters 150, 160 and 170 in the form of a filter wheel. Furthermore, the first observation filter can be set to 150, the second observation filter to 160. and / or the third observation filter 170 be a notch filter and / or a Notch filters are included. That is, the first observation filter is 150, the second... Observation filter 160 and / or the third observation filter 170 can be used in this way It should be set up so that it only reflects the illumination spectrum of the corresponding first lighting element 40, second lighting element 50 or third The lighting element 60 filters out the lighting unit 30. Image acquisition sensor 120 can include an NIR and / or RGB chip. The NIR chip can be specifically configured to detect all wavelengths from the minimum wavelength of visible light (approx. 320 nm) to the maximum NIR- to record wavelength range (approx. 2500 nm).

Claims

2022P00164WO - 18 - KARL STORZ SE & Co. KG Claims 1. Illumination device (10) for a medical fluorescence imaging system, comprising: an optical interface (20) for coupling illumination light to a medical imaging system; and an illumination unit (30) configured to supply illumination light to the optical interface, comprising: a first illumination element (40) configured to generate narrowband first illumination light with a first illumination spectrum for exciting at least two fluorescent dyes with different excitation spectra from a first spectral group (1), a second illumination element (50) configured to generate narrowband second illumination light with a second illumination spectrum for exciting at least two fluorescent dyes with different excitation spectra from a second spectral group (2), a third illumination element (60) configured toto generate narrowband third illumination light with a third illumination spectrum for exciting at least two fluorescent dyes with different excitation spectra from a third spectral group (3), wherein the illumination unit (30) is operable in three different illumination modes; and wherein the first illumination spectrum, the second illumination spectrum and the third illumination spectrum are spectrally disjoint.

2. Lighting device (10) according to claim 1, wherein in the three different lighting modes only one of the lighting elements (40, 50, 60) is activated.

3. Lighting device (10) according to one of claims 1 or 2, wherein the first lighting spectrum has an intensity maximum at a first wavelength (45); 2022P00164WO - 19 - KARL STORZ SE & Co. KG wherein the second illumination spectrum has an intensity maximum at a second wavelength (55); wherein the third illumination spectrum has an intensity maximum at a third wavelength (65); wherein the first wavelength (45) lies in a range of 445 nm to 500 nm; wherein the second wavelength (55) lies in a range of 630 nm to 670 nm; and wherein the third wavelength (65) lies in a range of 755 nm to 795 nm.

4. Lighting device (10) according to one of claims 1 to 3, wherein the first wavelength (45) is in a range of 445 nm to 485 nm.

5. Lighting device (10) according to claim 4, wherein the first wavelength (45) is in a range of 455 nm to 475 nm.

6. Lighting device (10) according to one of claims 1 to 3, wherein the first wavelength (45) is in a range of 460 nm to 500 nm.

7. Lighting device (10) according to claim 6, wherein the first wavelength (45) is in a range of 470 nm to 500 nm.

8. Lighting device (10) according to one of the preceding claims, wherein the second wavelength (55) is in a range of 640 nm to 660 nm.

9. Lighting device (10) according to one of the preceding claims, wherein the third wavelength (65) is in a range of 765 nm to 785 nm.

10. Lighting device (10) according to one of the preceding claims, wherein the lighting unit (30) further comprises a white light illuminating element (70) 2022P00164WO - 20 - KARL STORZ SE & Co. KG includes the lighting unit (30) which can be operated in a white light mode.

11. Lighting device (10) according to claim 10, wherein the lighting unit (30) is configured to switch between the white light mode and one of the three different lighting modes, in particular to flash.

12. Medical fluorescence imaging system (100) comprising: an illumination device (10) according to any of the preceding claims; and an imaging instrument (110) comprising: an image acquisition sensor (120) and an observation filter unit (140).

13. Medical fluorescence imaging system (100) according to claim 12, the observation filter unit (140) comprising: a first observation filter (150) comprising an edge filter that is transparent above a first edge wavelength and opaque below the first edge wavelength; and / or a second observation filter (160) that is transparent above a second edge wavelength and opaque below the second edge wavelength; and / or a third observation filter (170) that is transparent above a third edge wavelength and opaque below the third edge wavelength.

14. Medical fluorescence imaging system (100) according to claim 13, wherein the first, second and third edge wavelengths are of different sizes.

15. Medical fluorescence imaging system (100) according to one of claims 13 or 14, wherein the first edge wavelength is 10 to 20 nm larger than the first wavelength; and / or wherein the second edge wavelength is 10 to 20 nm larger than the second wavelength; and / or wherein the third edge wavelength is 10 to 20 nm larger than the third wavelength; 2022P00164WO - 21 - KARL STORZ SE & Co. KG 16. Medical fluorescence imaging system (100) according to any one of claims 13 to 15, wherein the first, second and / or third observation filter (150, 160, 170) is a bandpass filter or a notch filter.

17. Medical fluorescence imaging system (100) according to one of claims 12 to 16, wherein the observation filter unit (140) can be operated in three different observation modes, in each of which one of the observation filters (150, 160, 170) is arranged in front of the image acquisition sensor (120) with respect to a light incidence.

18. Medical fluorescence imaging system (100) according to one of claims 12 to 17, wherein the observation filter unit (140) has a further image acquisition sensor (130) by means of which the observation filter unit (140) can be operated in a white light observation mode.

19. Medical fluorescence imaging system (100) according to one of claims 17 or 18, wherein the white light observation mode and at least one of the observation modes can be operated simultaneously.

20. System (200) comprising: an illumination device (10) according to any one of claims 1 to 11 or a medical fluorescence imaging system (100) according to any one of claims 12 to 19; and at least two fluorescent dyes assigned to different spectral groups (1 , 2, 3).

21. System (200) according to claim 20, comprising three fluorescent dyes assigned to different spectral groups (1 , 2, 3).

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