Cosmetic composition for improving skin itching and atopy, cosmetics comprising same, and method for preparing same

Abstract

A cosmetic composition for alleviating skin itching and improving atopic symptoms, according to the present invention, comprises a eutectic mixture of glutamine and linoleic acid, and exhibits the following effects: increasing the skin penetration of glutamine and enhancing skin barrier function, thereby substantially alleviating atopic symptoms including itching; improving the water solubility of linoleic acid, thereby enhancing antioxidant activity and skin protection effects; providing synergistically enhanced effects on alleviating atopic symptoms including itching and enhancing skin protection, as compared to the use of glutamine or linoleic acid alone, without adverse effects; and achieving enhanced effects in alleviating atopic symptoms including itching and improving skin protection, without adverse effects associated with surfactants and oils.

Description

Cosmetic composition for improving skin itching and atopic dermatitis, cosmetic containing the same, and method for manufacturing the same

[0001] The present invention relates to a cosmetic composition for improving skin itching and atopic dermatitis, a cosmetic product containing the same, and a method for manufacturing the same. More specifically, the invention relates to a cosmetic composition for improving skin itching and atopic dermatitis that includes a eutectic mixture of glutamine and linoleic acid to increase the penetration of glutamine through the keratin and enhance the solubility of linoleic acid in water, thereby having improved effects on itching and atopic dermatitis, and having complex improved effects on promoting skin regeneration and alleviating inflammation, a cosmetic product containing the same, and a method for manufacturing the same.

[0002] The skin is the largest tissue in the body and serves as the primary organ for protecting and contacting the body against the external environment and stimuli. It not only acts as the first line of defense against external substances and stimuli but also plays a crucial role in internal metabolism by regulating body temperature and fluid balance. Furthermore, as the first organ visible to the outside, the skin is a vital component in expressing external beauty. In modern society, this emphasis on external beauty is increasingly becoming as significant as the emphasis on health. This is because, with the rising elderly population, physical beauty has established itself as a criterion symbolizing youth and health; moreover, for the younger generation, this external beauty manifests as confidence and serves as a competitive edge that influences their professional performance. For these reasons, active efforts are being made in both academic and industrial fields to prevent skin damage and aging.

[0003] Atopy is a type of dermatitis characterized by chronic, recurring skin eczema accompanied by severe itching. As one of the representative allergic diseases alongside asthma, allergic rhinitis, and chronic urticaria, atopy is known to result from the complex interplay of various factors, including genetic predisposition, environmental factors, immunological abnormalities, and defects in the skin's protective barrier; however, it remains difficult to definitively determine the exact cause of atopy. The most significant characteristics of atopy are severe itching and a highly sensitive reaction to external stimuli or allergens.

[0004] Recently, as the environment becomes increasingly polluted, skin diseases of unknown cause, most notably known as atopic dermatitis, are becoming more common. These conditions typically begin in infancy or childhood and persist for a long time or recur as the child grows, manifesting as symptoms such as dry skin, eczema, dermatitis, erythema, and itching. While there are treatment methods for atopic dermatitis involving the use of moisturizers to provide adequate hydration to dry skin and reduce itching and inflammation, or the use of antihistamines or steroids in severe cases, long-term use of antihistamines or steroids can lead to tolerance or increase skin hypersensitivity, causing redness, swelling, and acne even with mild irritation. Additionally, prolonged use can lead to damage to the immune system.

[0005] Various functional cosmetics are being released to prevent or alleviate the symptoms of atopic dermatitis and other skin diseases. However, conventional functional cosmetics have side effects that make the skin sensitive and dry, and because they are not efficiently absorbed into the skin, there have been problems in that skin-improving effects—such as alleviating atopic symptoms, moisturizing, antibacterial, and anti-inflammatory action—are not effectively manifested.

[0006] Furthermore, exposure to external factors such as excessive sunlight or environmental pollution can induce skin damage and inflammatory reactions, including itching. It is known that such skin damage and inflammatory reactions not only exacerbate atopic dermatitis and create aesthetic issues, but also cause various skin problems, such as secondary pigmentation and itching, due to substances generated during the inflammatory process. Recently, consumer interest in the field of atopic dermatitis has been increasing to address issues like itching and aesthetic concerns. While various products for improving atopic dermatitis are being developed, actual consumer confidence in their efficacy remains low. Although various cosmetic compositions are being researched to resolve these problems, conventionally known cosmetic compositions have limited usage amounts due to side effects upon application or have minimal penetration into the skin, making it practically impossible to expect any improvement. Therefore, there is an urgent need to develop new cosmetic compositions that possess comprehensive improvement effects for atopic dermatitis while being free from side effects compared to existing compositions.

[0007] In addition, in the case of conventional technology, there was a problem in that glutamine has difficulty penetrating the skin through the keratin, which limits its ability to effectively improve atopic symptoms, and in the case of linoleic acid, there was a problem in that it has low solubility in water, which limits its application in water-soluble formulations (toners, ampoules, essences, etc.). Furthermore, there was a problem in that there were limitations in applying a complex mixture of glutamine and linoleic acid, which have poor compatibility with each other, to the skin. Therefore, there is an urgent need to develop a new technology that can enhance the penetration of glutamine through the keratin and increase the water solubility of linoleic acid so that it can be easily applied to water-soluble formulations (toners, ampoules, essences, etc.), easily mix glutamine and linoleic acid for application to the skin, and expect a synergistic effect using a complex of glutamine and linoleic acid.

[0008] A cosmetic composition for improving dermatitis was disclosed in Korean Registered Patent Publication No. 10-0953900 (published on April 22, 2010). The invention relates to a cosmetic composition for improving dermatitis, and more specifically, to a composition for improving dermatitis and a skin whitening composition containing, as an active ingredient, a peptide formed by combining one or more amino acids with glutamine. More specifically, it is characterized by containing, as an active ingredient, a peptide formed by combining one or more amino acids with glutamine, which has a cPLA2 activity inhibitory effect, to improve atopic dermatitis, contact dermatitis, and dermatitis caused by ultraviolet rays, and thereby having a skin whitening effect and a hair loss prevention effect. The cosmetic composition for improving dermatitis described above is based on glutamine, a substance that inhibits the activity of cPLA2, an important enzyme in the inflammatory response, and is a peptide combined with one or more amino acids that has the effect of improving atopic dermatitis, contact dermatitis, and dermatitis caused by ultraviolet rays. However, it is difficult to expect an effect of improving atopic symptoms because it does not solve the problem of glutamine having difficulty penetrating the skin through the keratin, and there is a problem in that it is difficult to expect a complex synergistic effect for improving atopic symptoms and promoting skin regeneration using a complex of glutamine and linoleic acid.

[0009] Accordingly, the inventors have developed a cosmetic composition for improving skin itching and atopic dermatitis, a cosmetic product containing the same, and a method for manufacturing the same, which includes a eutectic mixture of glutamine and linoleic acid to increase the penetration of glutamine through the keratin of the skin, thereby having a substantial effect of improving atopic symptoms including itching, and improves moisturization and promotes skin regeneration by enhancing the solubility of linoleic acid in water, and has a complex synergistic effect of improving atopic symptoms including itching and promoting skin regeneration by using a eutectic mixture of glutamine and linoleic acid.

[0010] The present invention was devised to solve the above problems, and the objective of the present invention is to provide a cosmetic composition for improving skin itching and atopic dermatitis that uses a eutectic mixture of glutamine and linoleic acid to increase the penetration of glutamine through the keratin and enhance barrier improvement, thereby having a substantial effect of improving atopic symptoms including itching, a cosmetic product containing the same, and a method for manufacturing the same.

[0011] In addition, another objective of the present invention is to provide a cosmetic composition for improving skin itching and atopic dermatitis that improves the water solubility of linoleic acid using a eutectic mixture of glutamine and linoleic acid, and has antioxidant enhancement and skin protection improvement effects, a cosmetic product containing the same, and a method for manufacturing the same.

[0012] In addition, another objective of the present invention is to provide a cosmetic composition for improving skin itching and atopic dermatitis that reduces side effects compared to using glutamine and linoleic acid individually, and strengthens and enhances the skin protection effect and improves atopic symptoms including itching compared to using glutamine and linoleic acid individually, thereby having a strengthened and enhanced effect on improving atopic symptoms including itching and protecting the skin without side effects, a cosmetic product containing the same, and a method for manufacturing the same.

[0013] In addition, another objective of the present invention is to provide a cosmetic composition for improving skin itching and atopic dermatitis, a cosmetic containing the same, and a method for manufacturing the same, wherein the solubility in water is enhanced when using a eutectic mixture of glutamine and linoleic acid compared to when glutamine and linoleic acid are dissolved in water separately, and thus has an increased effect in improving atopic symptoms including itching and protecting the skin even when the same amount is applied to the skin.

[0014] In addition, another objective of the present invention is to provide a cosmetic composition for improving skin itching and atopic dermatitis, a cosmetic containing the same, and a method for manufacturing the same, which has enhanced and synergistic effects on improving atopic symptoms including itching and protecting the skin without side effects caused by surfactants and oil compositions, since a eutectic mixture of glutamine and linoleic acid is formed without a separate surfactant or oil composition.

[0015] To achieve the above objectives, a cosmetic composition for improving skin itching and atopic dermatitis according to one embodiment of the present invention may be composed of a eutectic mixture of glutamine and linoleic acid.

[0016] In this case, in a cosmetic composition for improving skin itching and atopic dermatitis according to another embodiment of the present invention, the cosmetic composition for improving skin itching and atopic dermatitis may be characterized by additionally including water and a linear diol having 5 to 8 carbon atoms that has hydroxyl groups at carbon 1 and carbon 2 and does not have a branched structure.

[0017] In this case, in a cosmetic composition for improving skin itching and atopic dermatitis according to another embodiment of the present invention, the linear diol may be characterized as being 1,2-hexanediol.

[0018] In this case, a cosmetic composition for improving skin itching and atopic dermatitis according to another embodiment of the present invention may be characterized in that the glutamine is 2-6 parts by weight, the linoleic acid is 0.4-4 parts by weight, the 1,2-hexanediol is 10-20 parts by weight, and the water is 70-87.6 parts by weight.

[0019] In this case, in a cosmetic composition for improving skin itching and atopic dermatitis according to another embodiment of the present invention, the cosmetic composition for improving skin itching and atopic dermatitis may be characterized by additionally including quercetin.

[0020] In this case, in a cosmetic composition for improving skin itching and atopic dermatitis according to another embodiment of the present invention, the cosmetic composition for improving skin itching and atopic dermatitis may be characterized by additionally including ethyl alcohol and dipropylene glycol.

[0021] In this case, a cosmetic composition for improving skin itching and atopic dermatitis according to another embodiment of the present invention may be characterized in that the glutamine is 2-6 parts by weight, the linoleic acid is 0.4-4 parts by weight, the 1,2-hexanediol is 10-20 parts by weight, the water is 65.97-83.63 parts by weight, the quercetin is 0.01-0.03 parts by weight, the ethyl alcohol is 2.61-2.63 parts by weight, and the dipropylene glycol is 1.35-1.37 parts by weight.

[0022] Meanwhile, a cosmetic for improving skin itching and atopic dermatitis according to another embodiment of the present invention may be composed of the cosmetic composition for improving skin itching and atopic dermatitis according to claims 1 to 7.

[0023] Meanwhile, a method for preparing a cosmetic composition for improving skin itching and atopic dermatitis according to another embodiment of the present invention comprises the steps of: generating a first solution in which glutamine is dissolved in water by stirring 2-6 parts by weight of glutamine and 65.97-83.63 parts by weight of water at 1,300 rpm - 2,000 rpm while heating to 40℃-90℃; generating a second solution in which linoleic acid is dissolved in 1,2-hexanediol by stirring 0.4-4 parts by weight of linoleic acid and 10-20 parts by weight of 1,2-hexanediol at 500 rpm - 1,000 rpm at room temperature; and adding the second solution to the first solution while heating to 40℃-90℃ and stirring at 3,000 rpm - It may be configured to include a step of generating a eutectic mixture of glutamine and linoleic acid by stirring at 6,000 rpm, or by introducing the second solution into the first solution and pressurizing and dispersing it at 4,000 psi to 35,000 psi.

[0024] In this case, a method for preparing a cosmetic composition for improving skin itching and atopic dermatitis according to another embodiment of the present invention comprises: a step of generating a first-1 solution in which glutamine is dissolved in water by stirring 2-6 parts by weight of glutamine and 70-87.6 parts by weight of water at 1,300 rpm - 2,000 rpm while heating to 40℃-90℃; a step of generating a second-1 solution in which linoleic acid is dissolved in 1,2-hexanediol by stirring 0.4-4 parts by weight of linoleic acid and 10-20 parts by weight of 1,2-hexanediol at 500 rpm - 1,000 rpm at room temperature; and 0.01-0.03 parts by weight of quercetin and ethyl alcohol The method may be configured to include a step of producing a third solution by mixing 2.61-2.63 parts by weight of and 1.35-1.37 parts by weight of dipropylene glycol, and a step of introducing the third solution while the second-1 solution is heated to 40°C-90°C and stirred at 3,000 rpm-6,000 rpm as the second-1 solution is introduced into the first-1 solution, or introducing the third solution while the second-1 solution is pressurized to 4,000 psi-35,000 psi and dispersed as the second-1 solution is introduced into the first-1 solution and the third solution is introduced as an intermediate, thereby producing a eutectic mixture of glutamine and linoleic acid supported with quercetin.

[0025] The cosmetic composition for improving skin itching and atopic dermatitis according to the present invention, the cosmetic product containing the same, and the method for manufacturing the same have the following effects.

[0026] First, the cosmetic composition for improving skin itching and atopic dermatitis according to the present invention, the cosmetic containing the same, and the method for manufacturing the same utilize a eutectic mixture of glutamine and linoleic acid to increase the penetration of glutamine into the skin and enhance barrier improvement, thereby having a substantial effect of improving atopic symptoms including itching.

[0027] Second, the cosmetic composition for improving skin itching and atopic dermatitis according to the present invention, the cosmetic containing the same, and the method for manufacturing the same utilize a eutectic mixture of glutamine and linoleic acid to enhance the water solubility of linoleic acid and have the effects of enhancing antioxidant activity and improving skin protection.

[0028] Third, the cosmetic composition for improving skin itching and atopic dermatitis according to the present invention, the cosmetic containing the same, and the method for manufacturing the same reduce side effects compared to when glutamine and linoleic acid are used individually, and strengthen and enhance the skin protection effect and the improvement of atopic symptoms including itching compared to when glutamine and linoleic acid are used individually, thereby having a strengthened and enhanced effect for improving atopic symptoms including itching and skin protection without side effects.

[0029] Fourth, the cosmetic composition for improving skin itching and atopic dermatitis according to the present invention, the cosmetic containing the same, and the method for manufacturing the same have improved solubility in water when a eutectic mixture of glutamine and linoleic acid is used compared to when glutamine and linoleic acid are dissolved in water individually, so that even when the same amount is applied to the skin, the effect of improving atopic symptoms including itching and protecting the skin is increased.

[0030] Fifth, the cosmetic composition for improving skin itching and atopic dermatitis according to the present invention, the cosmetic containing the same, and the method for manufacturing the same form a eutectic mixture of glutamine and linoleic acid without a separate surfactant or oil composition, so it has an enhanced and synergistic effect on improving atopic symptoms including itching and protecting the skin without side effects caused by surfactants and oil compositions.

[0031] FIG. 1 is a flowchart illustrating a method for preparing a cosmetic composition for improving skin itching and atopic dermatitis according to an embodiment of the present invention.

[0032] FIG. 2 is a flowchart illustrating a method for preparing a cosmetic composition for improving skin itching and atopic dermatitis, comprising the step of producing a eutectic mixture of glutamine and linoleic acid loaded with quercetin according to an embodiment of the present invention.

[0033] Figure 3 is a transmission electron microscope (TEM) image of a eutectic mixture of glutamine and linoleic acid according to an embodiment of the present invention and a eutectic mixture of glutamine and linoleic acid loaded with quercetin.

[0034] Figure 4 is a diagram showing the results of measuring 1H-NMR for Comparative Example 2 (deuterium).

[0035] Figure 5 is a diagram showing the results of measuring the 1H-NMR for Example 1 (deuterium).

[0036] Figure 6 is a diagram showing the FT-IR (Fourier-transform infrared spectroscopy) measurement results for Example 1.

[0037] Figure 7 is a diagram showing the Differential Scanning Calorimetry (DSC) measurement results for Example 1.

[0038] Figure 8 is a diagram showing the results of quantifying the concentrations of the suppressed inflammatory cytokines MCP-1 and IL-1α using an enzyme-linked immunosorbent assay (ELISA).

[0039] Figure 9 is a diagram showing the results of quantifying the concentrations of the suppressed inflammatory cytokines IL-4 and IL-6 using an enzyme-linked immunosorbent assay (ELISA).

[0040] Figure 10 is a diagram showing the results of quantifying the concentrations of the suppressed inflammatory cytokines IL-8 / CXCL-8 and TNF-α using an enzyme-linked immunosorbent assay (ELISA).

[0041] Figure 11 is a diagram showing the results of quantifying the concentrations of the suppressed inflammatory cytokines MMP-1 and MMP-9 using an enzyme-linked immunosorbent assay (ELISA).

[0042] In the present invention, the attached drawings may be illustrated with exaggerated expressions to distinguish it from the prior art, ensure clarity, and facilitate the understanding of the technology. Furthermore, the terms and words described below are defined considering their functions in the present invention; since these may vary depending on the intentions or conventions of the user or operator, the definitions of such terms and words should be based on the technical content throughout this specification. Moreover, terms and words used in this specification and claims are not limited to their ordinary or dictionary meanings; rather, based on the premise that the inventor may appropriately define the concepts of terms and words to best describe their invention, they should be interpreted in a meaning and concept consistent with the technical spirit of the present invention. Meanwhile, embodiments, experimental examples, or comparative examples are merely exemplary details of the components presented in the claims of the present invention and do not limit the scope of rights of the present invention; the scope of rights should be interpreted based on the technical spirit throughout the specification of the present invention. In addition, the embodiments, experimental examples, or comparative examples described in the specification of the present invention are merely the most preferred embodiments, experimental examples, or comparative examples of the present invention and do not represent all of the technical ideas of the present invention; therefore, it should be understood that various equivalents and modifications that can replace them may be possible or exist at the time of filing the application for the present invention.

[0043] Furthermore, prior to describing the present invention, it should be noted that matters not necessary to reveal the gist of the invention—namely, known configurations that a person skilled in the art with ordinary knowledge can obviously add—have not been explained or described in detail.

[0044] Hereinafter, preferred embodiments, experimental examples, and comparative examples of the present invention will be described.

[0045] A cosmetic composition for improving skin itching and atopic dermatitis according to one embodiment of the present invention may be composed of a eutectic mixture of glutamine and linoleic acid.

[0046] In the cosmetic composition for improving skin itching and atopic dermatitis according to the present invention, glutamine is known to be effective against inflammation and pruritus, but glutamine has limited solubility in water (25 g / L, ref. American Chemical Society, https: / www.acs.org / molecule-of-the-week / archive / g / l-glutamine.html) and is almost insoluble in oils or alcohols, making it difficult to apply high concentrations to general solvents constituting cosmetic compositions. In terms of the effect of improving atopic symptoms and skin using glutamine, there was a problem that there were limitations in the effect of improving atopic symptoms and skin because it was difficult to apply high concentrations of glutamine to cosmetic compositions. However, in the cosmetic composition for improving skin itching and atopic dermatitis according to the present invention, glutamine forms a eutectic mixture with linoleic acid, which increases solubility in water and enables the application of high-concentration glutamine to the cosmetic composition, thereby possessing excellent effects in improving atopic symptoms and skin. Furthermore, glutamine, an amino acid, is a representative zwitterion characterized by possessing both a positively charged amine group and a negatively charged carboxyl group under general skincare cosmetic formulation conditions (pH 3.0 - 8.0). Although the entire molecule of such a zwitterion exhibits nonpolarity, there is a problem in that it is difficult to deliver it to the skin through the nonpolar keratin, which is advantageous for the penetration of nonpolar, lipophilic substances, due to partial charges present in parts.Therefore, in the case of glutamine, which is a zwitterion, when applied to the skin at the same concentration, there is a problem that it is difficult to deliver it to the skin through the keratin compared to non-polar substances of the same concentration, and there is a problem that the effect of improving atopic symptoms and skin improvement is inferior compared to non-polar substances of the same concentration. However, in the cosmetic composition for improving skin itching and atopic dermatitis according to the present invention, glutamine forms a eutectic mixture with linoleic acid, so glutamine is easily delivered to the skin through non-polar keratin using the eutectic mixture, and even when glutamine of the same concentration is applied to the skin alone, it has the characteristic of having a superior effect of improving atopic symptoms and skin improvement.

[0047] In the cosmetic composition for improving skin itching and atopic dermatitis according to the present invention, linoleic acid is known to have an antioxidant effect derived from double bonds and a useful effect of protecting the skin surface by forming a skin barrier as a free fatty acid. However, linoleic acid is basically a useful ingredient, so there were limitations in applying it to water-based formulations (toners, ampoules, essences, etc.) that provide aesthetic pleasure. However, in the cosmetic composition for improving skin itching and atopic dermatitis according to the present invention, linoleic acid forms a eutectic mixture with glutamine, making it soluble in water. Since it is possible to apply linoleic acid together with glutamine in water-based cosmetic compositions, it is characterized by having an antioxidant effect and a useful effect of protecting the skin surface by forming a skin barrier as a free fatty acid.

[0048] The cosmetic composition for improving skin itching and atopic dermatitis according to the present invention forms a eutectic mixture of glutamine and linoleic acid without a separate surfactant and oil composition, and thus has an enhanced and synergistic effect on improving atopic symptoms and promoting skin regeneration without side effects caused by a separate surfactant and oil composition, and has an enhanced and synergistic effect on improving atopic symptoms and protecting the skin surface compared to when glutamine and linoleic acid are applied to the skin separately.

[0049] In the cosmetic composition for improving skin itching and atopic dermatitis according to the present invention, a eutectic mixture may be formed by hydrogen bonding or dipole-dipole interaction between the carboxyl group of linoleic acid and the amine group of glutamine. Furthermore, in the cosmetic composition for improving skin itching and atopic dermatitis according to the present invention, a eutectic mixture may be formed by hydrogen bonding or dipole-dipole interaction between the carboxyl group of linoleic acid and the cation group present in the side chain group (R group) of glutamine. In the cosmetic composition for improving skin itching and atopic dermatitis according to the present invention, the eutectic mixture of glutamine and linoleic acid can be formed in the pH range of pH 4 to pH 7, which is the general pH range of cosmetics or skin, so the cosmetic composition for improving skin itching and atopic dermatitis according to the present invention has the characteristic of being universally usable with various cosmetics and on the skin.

[0050] In a cosmetic composition for improving skin itching and atopic dermatitis according to another embodiment of the present invention, the cosmetic composition for improving skin itching and atopic dermatitis may be characterized by additionally including water and a linear diol having 5 to 8 carbon atoms that has hydroxyl groups at carbon 1 and carbon 2 and does not have a branched structure.

[0051] In the cosmetic composition for improving skin itching and atopic dermatitis according to the present invention, the cosmetic composition for improving skin itching and atopic dermatitis further comprises a linear diol having 5 to 8 carbon atoms, having hydroxyl groups at the 1st and 2nd carbons, and not having a branched structure, and water, thereby hydrogen bonding between the cation present in the amine group or side chain group (R group) of glutamine and the hydroxyl group of water, and after the carboxyl group of linoleic acid forms a hydrogen bond with the hydrogen of the linear diol having 5 to 8 carbon atoms, having hydroxyl groups at the 1st and 2nd carbons, and not having a branched structure, the carboxyl group of linoleic acid and the cation present in the amine group or side chain group (R group) of glutamine easily hydrogen bond (hydrogen A eutectic mixture can be formed through hydrogen bonding or dipole-dipole interaction, and a nanovesicle can be formed by the cation present in the carboxyl group of linoleic acid and the amine group or side chain group (R group) of glutamine acting as a hydrophilic head and a hydrophobic tail during the process of forming a eutectic mixture through hydrogen bonding or dipole-dipole interaction.In the process of forming nanovesicles having a hydrophilic head and a hydrophobic tail, a linear diol with 5 to 8 carbon atoms that has hydroxyl groups at carbons 1 and 2 and does not have a branched structure, which is used as a dispersant for linoleic acid, has a structure similar to a hydrophilic hydroxyl group configuration and a hydrophobic carbon skeleton configuration, and plays a role in increasing the stability of nanovesicles with a hydrophilic head and a hydrophobic tail structure formed by a eutectic mixture of glutamine and linoleic acid.

[0052] In a cosmetic composition for improving skin itching and atopic dermatitis according to another embodiment of the present invention, the linear diol may be characterized as being 1,2-hexanediol.

[0053] In the cosmetic composition for improving skin itching and atopic dermatitis according to the present invention, the linear diol is characterized as being 1,2-hexanediol, so that the cation present in the amine group or side chain group (R group) of glutamine forms a hydrogen bond with the hydroxyl group of water, and the carboxyl group of linoleic acid forms a hydrogen bond with the hydrogen of 1,2-hexanediol, and subsequently, the carboxyl group of linoleic acid and the cation present in the amine group or side chain group (R group) of glutamine easily form a hydrogen bond or dipole-dipole interaction to form a eutectic mixture, and the carboxyl group of linoleic acid and In the process where a cation group present in the amine group or side chain group (R group) of glutamine forms a eutectic mixture through hydrogen bonding or dipole-dipole interaction, it acts as a hydrophilic head and a hydrophobic tail to form nanovesicles. In the process of forming nanovesicles with a hydrophilic head and a hydrophobic tail, 1,2-hexanediol, which is used as a dispersant for linoleic acid, has a structure with a hydrophilic hydroxyl group configuration and a hydrophobic carbon skeleton configuration, and plays a role in enhancing the stability of nanovesicles with a hydrophilic head and a hydrophobic tail structure formed by the eutectic mixture of glutamine and linoleic acid.Nanovesicles with a hydrophilic head and a hydrophobic tail structure formed by a eutectic mixture of glutamine and linoleic acid, whose stability is enhanced by 1,2-hexanediol, can have various diameters, preferably 10-400 nm in diameter, more preferably 120-200 nm in diameter, and most preferably 164.6 nm in diameter. Nanovesicles with a hydrophilic head and a hydrophobic tail structure formed by a eutectic mixture of glutamine and linoleic acid, whose stability is enhanced by 1,2-hexanediol, can simultaneously possess the characteristics of increased solubility in polar water and easy delivery to the skin through non-polar keratin. Due to these characteristics, the eutectic mixture of glutamine and linoleic acid dissolves in polar water and is easily delivered to the skin through non-polar keratin, thereby simultaneously providing effects such as improved skin protection, improved skin barrier through the promotion of skin regeneration via improved skin permeability, relief of itching through inflammation alleviation, and improvement of atopic symptoms.In addition, nanovesicles with a hydrophilic head and a hydrophobic tail structure are formed by a eutectic mixture of glutamine and linoleic acid, whose stability is enhanced by 1,2-hexanediol, without the addition of a separate surfactant, and since the eutectic mixture of glutamine and linoleic acid dissolves in polar water and is easily delivered to the skin through non-polar keratin, the cosmetic composition for improving skin itching and atopic dermatitis according to the present invention can provide a fresh aesthetic pleasure without irritation and stickiness derived from surfactants, and minimizes skin irritation caused by surfactants, making it suitable for sensitive skin, atopic skin, and itchy skin. Furthermore, by composing a cosmetic composition for improving skin itching and atopic dermatitis without a separate oil composition, it is suitable for application to acne-prone or troubled skin that is susceptible to oil composition, and has the characteristic of minimizing the proliferation of anaerobic bacteria, including atopic dermatitis-causing bacteria (S. aureus), which may occur due to the shielding of the skin surface by the oil composition. 1,2-hexanediol does not participate in hydrogen bonding of the eutectic mixture of glutamine and linoleic acid, plays a role in stably dispersing linoleic acid to increase the probability of reaction between glutamine and linoleic acid to form the eutectic mixture, and plays a role in improving the stability of nanovesicles based on the eutectic mixture of glutamine and linoleic acid.

[0054] In a cosmetic composition for improving skin itching and atopic dermatitis according to another embodiment of the present invention, the glutamine may be 2-6 parts by weight, the linoleic acid may be 0.4-4 parts by weight, the 1,2-hexanediol may be 10-20 parts by weight, and the water may be 70-87.6 parts by weight.

[0055] In the cosmetic composition for improving skin itching and atopic dermatitis according to the present invention, glutamine may be included in an amount of 2 to 6 parts by weight. If glutamine is included in an amount of less than 2 parts by weight, a eutectic mixture is not sufficiently formed, so non-polar linoleic acid undergoes phase separation and fails to form a single phase, which may result in insufficient antioxidant enhancement and skin protection improvement effects by linoleic acid. If glutamine is included in an amount of more than 6 parts by weight, glutamine is not sufficiently dissolved in water, causing precipitation, and may result in insufficient substantial improvement of atopic symptoms by glutamine.

[0056] In the cosmetic composition for improving skin itching and atopic dermatitis according to the present invention, the linoleic acid may be included in an amount of 0.4 to 4 parts by weight. If the linoleic acid is included in an amount less than 0.4 parts by weight, a eutectic mixture is not sufficiently formed, so glutamine cannot be easily delivered to the skin through the non-polar keratin, and a problem may arise in which the barrier improvement by glutamine and the effect of improving actual atopic symptoms are insufficient. If the linoleic acid is included in an amount greater than 4 parts by weight, a eutectic mixture is not sufficiently formed, so the non-polar linoleic acid undergoes phase separation and fails to form a single phase, and a problem may arise in which the antioxidant enhancement and skin protection improvement effects by linoleic acid are insufficient.

[0057] In the cosmetic composition for improving skin itching and atopic dermatitis according to the present invention, the 1,2-hexanediol may be included in an amount of 10 to 20 parts by weight. If the 1,2-hexanediol is included in an amount less than 10 parts by weight, a eutectic mixture is not sufficiently formed, causing the non-polar linoleic acid to undergo phase separation and fail to form a single phase, which may result in insufficient antioxidant enhancement and skin protection improvement effects by linoleic acid. If the 1,2-hexanediol is included in an amount exceeding 20 parts by weight, the relative concentration of water decreases, and due to the low solubility of glutamine in 1,2-hexanediol, glutamine cannot easily penetrate the skin through the non-polar keratin. Problems may arise where it is not delivered, and the improvement of the barrier by glutamine and the actual improvement of atopic symptoms are insufficient.

[0058] In the cosmetic composition for improving skin itching and atopic dermatitis according to the present invention, the water may be included in an amount of 70 to 87.6 parts by weight. If the water is included in an amount of less than 70 parts by weight, glutamine is not sufficiently included, so glutamine is not easily delivered to the skin through the non-polar keratin, and problems may arise in which the barrier improvement by glutamine and the substantial improvement effect of atopic symptoms are insufficient. If the water is included in an amount of more than 87.6 parts by weight, the concentration of 1,2-hexanediol is relatively reduced and a eutectic mixture is not sufficiently formed, so the non-polar linoleic acid undergoes phase separation and fails to form a single phase, which may result in problems in which the antioxidant enhancement and skin protection improvement effects by linoleic acid are insufficient.

[0059] In a cosmetic composition for improving skin itching and atopic dermatitis according to another embodiment of the present invention, the cosmetic composition for improving skin itching and atopic dermatitis may be characterized by additionally including quercetin.

[0060] In a cosmetic composition for improving skin itching and atopic dermatitis according to another embodiment of the present invention, quercetin is additionally included in the cosmetic composition for improving skin itching and atopic dermatitis, thereby providing skin soothing and inflammation suppression (inhibition of inflammatory factor cytokines) effects based on antioxidant efficacy, and also providing an effect for improving atopic symptoms. Quercetin has the characteristic of being insoluble in water, is compatible with only specific oils, and is insoluble in 1,2-hexanediol. Therefore, there were limitations in applying quercetin to water-based formulations (toners, ampoules, essences, etc.) that provide aesthetic pleasure. However, since quercetin is compatible with linoleic acid, it is possible to apply linoleic acid loaded with quercetin together with glutamine in a water-based cosmetic composition by mixing and loading quercetin onto linoleic acid, forming a eutectic mixture with glutamine using linoleic acid loaded with quercetin, and making it soluble in water. As a result, a cosmetic composition for improving skin itching and atopic dermatitis that additionally includes quercetin can have skin soothing and inflammation suppression (inhibition of inflammatory factor cytokines) effects based on the antioxidant efficacy of quercetin, as well as an effect for improving atopic symptoms.

[0061] In a cosmetic composition for improving skin itching and atopic dermatitis according to another embodiment of the present invention, the cosmetic composition for improving skin itching and atopic dermatitis may be characterized by additionally including ethyl alcohol and dipropylene glycol.

[0062] In a cosmetic composition for improving skin itching and atopic dermatitis according to another embodiment of the present invention, the cosmetic composition for improving skin itching and atopic dermatitis further comprises ethyl alcohol and dipropylene glycol, thereby dispersing quercetin in ethyl alcohol and dipropylene glycol, and mixing ethyl alcohol in which quercetin is dispersed with 1,2-hexanediol in which linoleic acid is dispersed with dipropylene glycol, so that linoleic acid supported with quercetin can be formed, and glutamine and linoleic acid supported with quercetin can be used to... A eutectic mixture can be formed.

[0063] The cosmetic composition for improving skin itching and atopic dermatitis according to the present invention may be characterized by comprising 2-6 parts by weight of glutamine, 0.4-4 parts by weight of linoleic acid, 10-20 parts by weight of 1,2-hexanediol, 65.97-83.63 parts by weight of water, 0.01-0.03 parts by weight of quercetin, 2.61-2.63 parts by weight of ethyl alcohol, and 1.35-1.37 parts by weight of dipropylene glycol.

[0064] In the cosmetic composition for improving skin itching and atopic dermatitis according to the present invention, glutamine may be included in an amount of 2 to 6 parts by weight. If glutamine is included in an amount of less than 2 parts by weight, a eutectic mixture is not sufficiently formed, so non-polar linoleic acid undergoes phase separation and fails to form a single phase, which may result in insufficient antioxidant enhancement and skin protection improvement effects by linoleic acid. If glutamine is included in an amount of more than 6 parts by weight, glutamine is not sufficiently dissolved in water, causing precipitation, and may result in insufficient substantial improvement of atopic symptoms by glutamine. In the cosmetic composition for improving skin itching and atopic dermatitis according to the present invention, the linoleic acid may be included in an amount of 0.4 to 4 parts by weight. If the linoleic acid is included in an amount less than 0.4 parts by weight, a eutectic mixture is not sufficiently formed, so glutamine cannot be easily delivered to the skin through the non-polar keratin, and a problem may arise in which the barrier improvement by glutamine and the effect of improving actual atopic symptoms are insufficient. If the linoleic acid is included in an amount greater than 4 parts by weight, a eutectic mixture is not sufficiently formed, so the non-polar linoleic acid undergoes phase separation and fails to form a single phase, and a problem may arise in which the antioxidant enhancement and skin protection improvement effects by linoleic acid are insufficient.In the cosmetic composition for improving skin itching and atopic dermatitis according to the present invention, the 1,2-hexanediol may be included in an amount of 10 to 20 parts by weight. If the 1,2-hexanediol is included in an amount less than 10 parts by weight, a eutectic mixture is not sufficiently formed, causing the non-polar linoleic acid to undergo phase separation and fail to form a single phase, which may result in insufficient antioxidant enhancement and skin protection improvement effects by linoleic acid. If the 1,2-hexanediol is included in an amount exceeding 20 parts by weight, the relative concentration of water decreases, and due to the low solubility of glutamine in 1,2-hexanediol, glutamine cannot easily penetrate the skin through the non-polar keratin. Problems may arise where it is not delivered, and the enhancement of barrier improvement by glutamine and the substantial improvement of atopic symptoms are insufficient. In the cosmetic composition for improving skin itching and atopic dermatitis according to the present invention, the water may be included in an amount of 65.97 to 83.63 parts by weight. If the water is included in an amount less than 65.97 parts by weight, glutamine is not sufficiently included, so glutamine is not easily delivered to the skin through the non-polar keratin, and problems may arise where the enhancement of barrier improvement by glutamine and the substantial improvement of atopic symptoms are insufficient. Furthermore, if the water is included in an amount of 83.If included in an amount exceeding 63 parts by weight, the relative concentration of 1,2-hexanediol decreases and a eutectic mixture is not sufficiently formed, resulting in the non-polar linoleic acid undergoing phase separation and failing to form a single phase, which may lead to insufficient antioxidant enhancement and skin protection improvement effects due to linoleic acid. If the quercetin is included in an amount less than 0.01, problems may arise in which the skin soothing and inflammation suppression (inhibition of inflammatory factor cytokines) effects based on antioxidant efficacy and the improvement of atopic symptoms are insufficient. If the quercetin is included in an amount exceeding 0.03 parts by weight, problems may arise in which the quercetin is not sufficiently loaded by mixing with linoleic acid and precipitates, leading to reduced efficiency. If the above range of 2.61-2.63 parts by weight of ethyl alcohol and 1.35-1.37 parts by weight of dipropylene glycol is exceeded, ethyl alcohol and dipropylene glycol dispersed with quercetin are not sufficiently formed, which causes problems in the formation of linoleic acid supported with quercetin, and problems may arise in the skin soothing and inflammation suppression (inhibition of inflammatory factor cytokines) effects and the improvement of atopic symptoms based on the antioxidant efficacy of linoleic acid supported with quercetin.

[0065] A cosmetic for improving skin itching and atopic dermatitis according to another embodiment of the present invention may be composed of the cosmetic composition for improving skin itching and atopic dermatitis according to claims 1 to 7.

[0066] Examples of cosmetic formulations for improving skin itching and atopic dermatitis to which the above cosmetic composition for improving skin itching and atopic dermatitis can be applied include skin toner formulations, lotion formulations, cream formulations, etc.

[0067] FIG. 1 is a flowchart illustrating a method for preparing a cosmetic composition for improving skin itching and atopic dermatitis according to an embodiment of the present invention.

[0068] According to FIG. 1, a method for preparing a cosmetic composition for improving skin itching and atopic dermatitis according to another embodiment of the present invention comprises the steps of: a step of producing a first solution in which glutamine is dissolved in water by stirring 2-6 parts by weight of glutamine and 65.97-83.63 parts by weight of water at 1,300 rpm - 2,000 rpm while heating to 40℃-90℃; a step of producing a second solution in which linoleic acid is dissolved in 1,2-hexanediol by stirring 0.4-4 parts by weight of linoleic acid and 10-20 parts by weight of 1,2-hexanediol at 500 rpm - 1,000 rpm at room temperature; and a step of adding the second solution to the first solution while heating to 40℃-90℃. It may be configured to include a step of generating a eutectic mixture of glutamine and linoleic acid by stirring at 3,000 rpm - 6,000 rpm, or by introducing the second solution into the first solution and pressurizing and dispersing it at 4,000 psi - 35,000 psi.

[0069] In the method for preparing a cosmetic composition for improving skin itching and atopic dermatitis according to the present invention, if the temperature of the step in which a first solution in which glutamine is dissolved in water is produced is less than 40°C, there is a problem that glutamine is not sufficiently dissolved in water, and if it exceeds 90°C, there is a problem that water evaporates and glutamine precipitates. A disperser stirrer may be used for stirring in the step in which a first solution in which glutamine is dissolved in water is produced, but is not limited thereto. If the stirring speed is less than 1,300 rpm in the step in which a first solution in which glutamine is dissolved in water is produced, a problem may occur that glutamine is not sufficiently dissolved in water, and if the stirring speed exceeds 2,000 rpm, a problem may occur in efficiency. In addition, a disperse stirrer may be used for stirring in the step in which a second solution in which linoleic acid is dissolved in 1,2-hexanediol is produced, but is not limited thereto. If the stirring speed is less than 500 rpm in the step in which the second solution in which linoleic acid is dissolved in 1,2-hexanediol is produced, a problem may occur in which linoleic acid is not sufficiently dissolved in 1,2-hexanediol, and if the stirring speed exceeds 1,000 rpm, a problem may occur in efficiency. Furthermore, if the temperature at the stage where the eutectic mixture of glutamine and linoleic acid is formed is less than 40°C, there is a problem that the eutectic mixture of glutamine and linoleic acid is not sufficiently formed, and if it exceeds 90°C, there is a problem that water evaporates and glutamine precipitates.A homogenizer may be used for stirring during the stage where a eutectic mixture of glutamine and linoleic acid is produced, but is not limited thereto. If the stirring speed is less than 3,000 rpm during the stage where a eutectic mixture of glutamine and linoleic acid is produced, a problem may arise where the eutectic mixture of glutamine and linoleic acid is not sufficiently produced, and if the stirring speed exceeds 6,000 rpm, problems with efficiency and safety may arise. In addition, when the second solution is introduced into the first solution at the stage where the eutectic mixture of glutamine and linoleic acid is produced, if the pressure at which it is pressurized and dispersed is less than 4,000 psi, sufficient dispersion may not occur, and thus a problem may arise in which the eutectic mixture of glutamine and linoleic acid is not sufficiently produced. Also, when the second solution is introduced into the first solution at a pressure at which it is pressurized and dispersed exceeds 35,000 psi, the pressure may be excessive, and a problem with efficiency may arise. In the step where a eutectic mixture of glutamine and linoleic acid is formed, the second solution is introduced into the first solution and passed through a microfluidizer 1 to 3 times while pressurizing to 4,000 psi to 35,000 psi, and the step where a eutectic mixture of glutamine and linoleic acid is formed may proceed.

[0070] FIG. 2 is a flowchart illustrating a method for preparing a cosmetic composition for improving skin itching and atopic dermatitis, comprising the step of producing a eutectic mixture of glutamine and linoleic acid loaded with quercetin according to an embodiment of the present invention.

[0071] According to FIG. 2, a method for preparing a cosmetic composition for improving skin itching and atopic dermatitis according to another embodiment of the present invention comprises the steps of: generating a first-1 solution in which glutamine is dissolved in water by stirring 2-6 parts by weight of glutamine and 70-87.6 parts by weight of water at 1,300 rpm - 2,000 rpm while heating to 40℃-90℃; generating a second-1 solution in which linoleic acid is dissolved in 1,2-hexanediol by stirring 0.4-4 parts by weight of linoleic acid and 10-20 parts by weight of 1,2-hexanediol at room temperature at 500 rpm - 1,000 rpm; and generating 0.01-0.03 parts by weight of quercetin and ethyl alcohol. The method may be configured to include a step of producing a third solution by mixing 2.61-2.63 parts by weight of alcohol and 1.35-1.37 parts by weight of dipropylene glycol, and a step of introducing the third solution while the second-1 solution is heated to 40-90°C and stirred at 3,000-6,000 rpm as the second-1 solution is introduced into the first-1 solution, or introducing the third solution while the second-1 solution is pressurized to 4,000-35,000 psi and dispersed as the second-1 solution is introduced into the first-1 solution and the third solution is introduced as an intermediate, thereby producing a eutectic mixture of glutamine and linoleic acid supported with quercetin.

[0072] In the method for preparing a cosmetic composition for improving skin itching and atopic dermatitis according to the present invention, if the temperature of the step in which the first-1 solution in which glutamine is dissolved in water is produced is less than 40°C, there is a problem that glutamine is not sufficiently dissolved in water, and if it exceeds 90°C, there is a problem that glutamine precipitates due to water evaporation. A disperser stirrer may be used for stirring in the step in which the first-1 solution in which glutamine is dissolved in water is produced, but is not limited thereto. If the stirring speed in the step in which the first-1 solution in which glutamine is dissolved in water is produced is less than 1,300 rpm, a problem may occur in which glutamine is not sufficiently dissolved in water, and if the stirring speed exceeds 2,000 rpm, a problem may occur in efficiency. In addition, stirring in the step where the 2-1 solution in which linoleic acid is dissolved in 1,2-hexanediol is produced may be performed using a disperse stirrer, but is not limited thereto. If the stirring speed is less than 500 rpm in the step where the 2-1 solution in which linoleic acid is dissolved in 1,2-hexanediol is produced, a problem may occur in which the linoleic acid is not sufficiently dissolved in 1,2-hexanediol, and if the stirring speed exceeds 1,000 rpm, a problem with efficiency may occur. Furthermore, no special process conditions are required in the step where the 3 solution is produced. In addition, if the temperature of the step in which a eutectic mixture of glutamine and linoleic acid supported by quercetin is formed is less than 40°C, there is a problem that the eutectic mixture of glutamine and linoleic acid supported by quercetin is not sufficiently formed, and if it is more than 90°C, there is a problem that water evaporates and glutamine precipitates.A homogenizer may be used for stirring during the step in which a eutectic mixture of glutamine and linoleic acid supported by quercetin is produced, but is not limited thereto. If the stirring speed is less than 3,000 rpm during the step in which a eutectic mixture of glutamine and linoleic acid supported by quercetin is produced, a problem may occur in which the eutectic mixture of glutamine and linoleic acid supported by quercetin is not sufficiently produced, and if the stirring speed exceeds 6,000 rpm, problems with efficiency and safety may occur. In the step where a eutectic mixture of glutamine and linoleic acid loaded with quercetin is produced, the 2-1 solution is added to the 1-1 solution, heated to 40°C-90°C, and stirred at 3,000 rpm-6,000 rpm, and the 3 solution may be added in between at a time interval. In addition, when the eutectic mixture of glutamine and linoleic acid loaded with quercetin is produced, if the pressure at which the 2-1 solution is introduced into the 1-1 solution and dispersed is less than 4,000 psi, sufficient dispersion may not occur, and thus a problem may arise in which the eutectic mixture of glutamine and linoleic acid loaded with quercetin is not sufficiently produced. Also, if the pressure at which the 2-1 solution is introduced into the 1-1 solution and dispersed is more than 35,000 psi, the pressure may be excessive, and a problem with efficiency may arise.In the step where a eutectic mixture of glutamine and linoleic acid loaded with quercetin is produced, the 2-1 solution is introduced into the 1-1 solution, pressurized to 4,000 psi-35,000 psi and dispersed, and the 3 solution can be introduced in between with a time difference. In the step where a eutectic mixture of glutamine and linoleic acid loaded with quercetin is produced, the 2-1 solution is introduced into the 1-1 solution and can be passed through a microfluidizer a total of 1 to 3 times while pressurized to 4,000 psi to 35,000 psi, and the 3 solution may be introduced in between after passing through the microfluidizer once, or the 3 solution may be introduced in between after passing through the microfluidizer twice, so that the step of producing a eutectic mixture of glutamine and linoleic acid loaded with quercetin may proceed.

[0073] [Example 1, Example 2]

[0074] Composition Example 1 (wt%) Example 2 (wt%) Purified Water 77.00 73.76 Glutamine 5.00 4.80 Linoleic Acid 2.00 2.08 1,2-Hexanediol 16.00 15.36 Quercetin -0.02 Ethyl Alcohol -2.62 Dipropylene Glycol -1.36 Total 100.00 100.00

[0075] According to the method for preparing a cosmetic composition for improving skin itching and atopic dermatitis of FIG. 1, a cosmetic composition for improving skin itching and atopic dermatitis was prepared by including a eutectic mixture of glutamine and linoleic acid of Example 1, comprising 5.00 parts by weight of glutamine, 2 parts by weight of linoleic acid, 16 parts by weight of 1,2-hexanediol, and 77.00 parts by weight of water. In addition, according to a method for preparing a cosmetic composition for improving skin itching and atopic dermatitis, comprising the step of generating a eutectic mixture of glutamine and linoleic acid supported with quercetin of FIG. 2, the glutamine supported with quercetin and linoleic acid of Example 2 comprises 4.80 parts by weight of glutamine, 2.08 parts by weight of linoleic acid, 15.36 parts by weight of 1,2-hexanediol, 73.76 parts by weight of water, 0.02 parts by weight of quercetin, 2.62 parts by weight of ethyl alcohol, and 1.36 parts by weight of dipropylene glycol. A cosmetic composition for improving skin itching and atopic dermatitis was prepared containing a eutectic mixture of glutamine acid. Both Example 1 and Example 2 had a eutectic mixture structure having eutectic sites formed by hydrogen bonds and dipole-dipole interactions between glutamine and linoleic acid, and it was confirmed that nanovesicles based on the eutectic mixture were formed.It was confirmed that the compatibility between two incompatible materials (glutamine and linoleic acid) is significantly increased by the nanovesicle structure formed based on a eutectic mixture, making it possible to manufacture cosmetics without surfactants and oil compositions. Furthermore, compared to the anti-inflammatory and barrier improvement effects exhibited by glutamine and linoleic acid individually, the anti-inflammatory and barrier improvement effects exhibited by the eutectic mixture structure having eutectic sites formed by glutamine and linoleic acid are significantly increased. Due to this complex synergistic effect, it is possible to manufacture cosmetics suitable for sensitive, acne-prone, and atopic skin. Additionally, it is possible to apply this to various formulations, including skin toner, lotion, cream, mask, and pad formulations, thereby maximizing aesthetic pleasure and efficacy.

[0076] [Experimental Example 1] Determination of the Solubility of a Eutectic Mixture in Water

[0077] Glutamine is an amphoteric substance and is a polar, uncharged amino acid with an overall pI (isoelectric point) value of 5.65. Linoleic acid is a non-polar charged structure consisting of a negatively charged carboxyl group and a long carbon chain; when these two substances form hydrogen bonds, a eutectic point appears, and the total charge becomes negative. The eutectic mixture formed in this way can consist of a hydrophilic part, which is larger overall due to hydrogen bonding between the cation present in the amine group or side chain group (R group) of glutamine and the carboxyl group of linoleic acid, and a lipophilic part having the long carbon chain of linoleic acid. When glutamine is dissolved in water alone, due to the amphoteric nature of glutamine, hydrogen bonds are formed between glutamine and glutamine molecules and between glutamine and water molecules. It is believed that the eutectic mixture of glutamine and linoleic acid increases the solubility of glutamine due to the increased probability of hydrogen bonds between the enlarged hydrophilic part of glutamine and water molecules. Although linoleic acid is insoluble in water, it acquires solubility in water through the formation of a eutectic mixture with glutamine. According to the cosmetic composition for improving skin itching and atopic dermatitis containing the eutectic mixture of glutamine and linoleic acid of Example 1, it was confirmed that the solubility of glutamine and linoleic acid in water increased.Although glutamine was found to have limited solubility in water (25 g / L, ref. American Chemical Society, https: / www.acs.org / molecule-of-the-week / archive / g / l-glutamine.html), it was confirmed that the solubility of glutamine in water (more than 60 g / L) increased by more than 2.4 times by forming a eutectic mixture of glutamine and linoleic acid. In addition, although linoleic acid was found to have the characteristic of being insoluble in water, it was confirmed that the solubility of linoleic acid in water increased by more than 4 g / L by forming a eutectic mixture of glutamine and linoleic acid.

[0078] [Comparative Example 1, Comparative Example 2]

[0079] 1) A glutamine mixture (Comparative Example 1) was prepared by adding 5 parts by weight of glutamine and 77 parts by weight of water, mixing thoroughly by force stirring at 70 degrees, and then cooling to room temperature; 2) A simple mixture of glutamine, linoleic acid, and 1,2-hexanediol (Comparative Example 2) was prepared by adding 5 parts by weight of glutamine and 77 parts by weight of water, mixing thoroughly by force stirring at 70 degrees, and then cooling to room temperature, and then mixing 2 parts by weight of linoleic acid and 16 parts by weight of 1,2-hexanediol by stirring into the glutamine mixture.

[0080] [Experimental Example 2] Verification of a eutectic mixture through structural analysis and macroscopic stability

[0081] In the case of the glutamine mixture (Comparative Example 1), it was confirmed that over-melted glutamine precipitated when left to stand at room temperature for 1 day. In the case of the simple mixture of glutamine, linoleic acid, and 1,2-hexanediol (Comparative Example 2), it was confirmed that glutamine and linoleic acid were temporarily dispersed, but it was confirmed that they were not dissolved through the overall opaque phase, and it was confirmed that linoleic acid separated into phases after 3 days. However, the cosmetic composition for improving skin itching and atopic dermatitis containing the eutectic mixture of glutamine and linoleic acid of Example 1 exhibited excellent macroscopic stability. Even when placed in a transparent 100ml container and left at room temperature for 3 months, the eutectic mixture of glutamine and linoleic acid remained intact without phase separation, and macroscopic stability was maintained for more than 3 months. When left at room temperature under conditions without light shielding for 3 months, it was observed that in the case of the cosmetic composition for improving skin itching and atopic dermatitis containing the eutectic mixture of glutamine and linoleic acid of Example 1, the linoleic acid discolored due to oxidation, causing the overall color to turn yellow. However, it can be seen that the oxidation of linoleic acid in such a eutectic mixture does not affect the macroscopic stability of the cosmetic composition for improving skin itching and atopic dermatitis containing the eutectic mixture of glutamine and linoleic acid of Example 1.In addition, by observing that the yellow color was evenly distributed and discolored throughout the cosmetic composition for improving skin itching and atopic dermatitis containing the eutectic mixture of glutamine and linoleic acid of Example 1, it was confirmed that the oxidized linoleic acid remained stably dissolved in water through the eutectic mixture structure and that no phase separation occurred.

[0082] [Experimental Example 3] Transmission Electron Microscopy (TEM) imaging, measurement of diameter and polydispersity index using dynamic light scattering (DLS), and measurement of surface charge via zeta potential

[0083] Diameter (nm) Polydispersity Index (PDI) Zeta Potential (mV) Example 1 164 ± 3.10.116-4.56

[0084] Transmission electron microscopy (TEM) was performed on nanovesicles formed based on a eutectic mixture within a cosmetic composition for improving skin itching and atopic dermatitis containing the eutectic mixture of glutamine and linoleic acid of Example 1 (see Fig. 3), and the diameter and polydispersity index were measured using dynamic light scattering (DLS), and the surface charge was measured via zeta potential. The TEM measurements were performed using a JEM-2010 (JEOL, Japan) instrument with a 200 mesh carbon-coated copper grid. Dynamic light scattering (DLS) and zeta potential measurements were performed using the ELSZ-Neo model from Otsuka Electronics, and the same sample was measured three times at 25°C. After sample preparation, the sample was placed in a column for zeta potential measurement, and the measurement was started within 120 seconds to obtain the results. As a result, it was confirmed that Example 1 formed nanovesicles with an average size of 164 ± 3.1 nm and exhibited a surface charge of -4.56 mV. These structural characteristics of the nanovesicles enhance water stability distinct from simple mixtures and support improved skin improvement effects compared to the individual components of glutamine and linoleic acid through improved skin permeability of glutamine and linoleic acid.

[0085] [Experimental Example 4] Transmission Electron Microscopy (TEM) Imaging and High-Performance Liquid Chromatography (HPLC) Analysis of Example 2

[0086] Transmission electron microscopy (TEM) was performed on nanovesicles formed based on a eutectic mixture of glutamine and linoleic acid loaded with quercetin in a cosmetic composition for improving skin itching and atopic dermatitis containing a eutectic mixture of glutamine and linoleic acid loaded with quercetin of Example 2 (see Fig. 3), and the loading rate was measured using HPLC (High-Performance Liquid Chromatography). The diameter of the nanovesicles formed based on a eutectic mixture of glutamine and linoleic acid loaded with quercetin was measured to be an average of 213.2 ± 1.5 nm, and the loading rate of the eutectic mixture of glutamine and linoleic acid loaded with quercetin [loading rate = {1 - (quercetin content in unloaded sample / initial quercetin content)} * 100 (%)] was confirmed to be 62.23 ± 2.1%.

[0087] [Experimental Example 5] 1H-NMR (1H-Nuclear Magnetic Resonance) Analysis

[0088] For 1H-NMR analysis, Example 1 (deuterium) and Comparative Example 2 (deuterium) were prepared by replacing purified water with 99 atom% D2O (Sigma-Aldrich) having a structure substituted with deuterium. Example 1 (deuterium) and Comparative Example 2 (deuterium) were measured at 25°C using a 500 MHz Avance III-500 (NMR spectrometer, Bruker Instruments, Billerica, MA, USA). Tetramethylsilane was used as an internal standard. The results of the 1H-NMR measurements for Comparative Example 2 (deuterium) and Example 1 (deuterium) are shown in Figures 4 and 5.

[0089] As a result of the analysis of the 1H-NMR peaks performed prior, it was confirmed that the positions of the peaks of linoleic acid and 1,2-hexanediol, respectively, and the peaks of linoleic acid dispersed in 1,2-hexanediol were identical, confirming that 1,2-hexanediol simply acts as a dispersion medium for linoleic acid.

[0090] Figure 4 is a diagram showing the results of measuring 1H-NMR for Comparative Example 2 (deuterium).

[0091] Figure 5 is a diagram showing the results of measuring the 1H-NMR for Example 1 (deuterium).

[0092] According to Figure 4, although the exact peak position of linoleic acid was not detected, it was confirmed that no shift in the linoleic acid peak was observed when comparing qualitative positions. Furthermore, no shift in the glutamine peak was observed either. Therefore, it is determined that there are no interbonds, such as hydrogen bonds, between glutamine and linoleic acid or between linoleic acid and 1,2-hexanediol in the simple mixture of glutamine, linoleic acid, and 1,2-hexanediol of Comparative Example 2 (deuterium).

[0093] According to Figure 5, it was confirmed that the C, D, and E protons of 1,2-hexanediol were downshifted (proton deshielded) to a maximum level of 0.122 ppm in Example 1 (deuterium), which is attributed to the electron delocalization caused by the hydroxyl group of 1,2-hexanediol forming a hydrogen bond. This result is in contrast to Comparative Example 2 (deuterium), a simple mixture of the same composition that simply acts as a dispersion medium for linoleic acid, in which 1,2-hexanediol does not participate in any bonding. Therefore, in Example 1 (deuterium), glutamine and linoleic acid form a eutectic mixture, and 1,2-hexanediol participates in the nanovesicle structure formed based on the eutectic mixture. This implies that the participation of 1,2-hexanediol in the nanovesicle formation process contributes to the overall improvement of the stability of the nanovesicle structure formed based on the eutectic mixture. It was confirmed that Peak A of linoleic acid disappeared. This indicates that when linoleic acid is dispersed in DMSO, the proton H of the carboxyl group + Although it did not dissociate, while forming a eutectic mixture, the carboxyl group forms a hydrogen bond and the proton H +It is determined that the peak disappeared due to dissociation. The lower peak C remains unchanged, and the degree of downshift decreases in the order of lower peak D (0.281 ppm) > peak F (0.121 ppm) > peak E (0.032 ppm), which means that electron transfer occurs more significantly closer to the carboxyl group participating in hydrogen bonding. It was confirmed that the upper peak A of glutamine is upshifted by 0.236 ppm, and this result is caused by the formation of hydrogen bonds between the amine in glutamine and the carboxyl group of linoleic acid. The upper peak B of glutamine remained unchanged, and it was confirmed that the upper peak C of glutamine is slightly downshifted. This phenomenon occurs because glutamine and linoleic acid form a eutectic mixture.

[0094] [Experimental Example 6] FT-IR (Fourier-transform infrared spectroscopy) analysis (see Fig. 6)

[0095] FT-IR analysis was performed at 1 cm per point using Vertex70, Hyperion 2000 (FTIR spectrophotometer, Bruker Instruments). -1 4000~500cm at the data collection speed -1The spectral range was measured and analyzed. Changes in the amine functional group of glutamine and the carboxyl group of linoleic acid were confirmed through FT-IR analysis. While the position and shape of each peak remained unchanged in the simple mixture of glutamine and linoleic acid, a distinct change in shape was observed in the eutectic mixture of glutamine and linoleic acid.

[0096] [Experimental Example 7] Differential Scanning Calorimetry (DSC) Analysis (See Fig. 7)

[0097] Thermal characteristics of Example 1 and Comparative Example 2 were measured using a Differential Scanning Calorimetry (DSC, Mettler-Toledo) instrument. The accuracy of heat flow and temperature was 0.0001 mW and 0.01 K, respectively. Measurements were taken at 20 cc min -1 flow rate and 10 K min -1It was performed in a nitrogen atmosphere at a heating rate. After measurement, the results were plotted on a graph using analysis software (STARe, Mettler-Toledo). The difference between a simple mixture of glutamine and linoleic acid and a eutectic mixture of glutamine and linoleic acid, confirmed by thermal analysis using Differential Scanning Calorimetry (DSC, Mettler-Toledo), is as follows. When observing the 79.35 - 103.39°C range while heating the samples of Example 1 and Comparative Example 2, the endothermic reaction having a peak at 97.59°C is determined to be the region where water vaporization proceeds. In Comparative Example 2, a simple mixture of glutamine and linoleic acid, no peak appeared in the range of 140.01 - 189.73℃, but in Example 1, a eutectic mixture of glutamine and linoleic acid, a peak at 149.59℃ appeared in the range of 140.01 - 189.73℃, and this is determined to be the eutectic point of Example 1, a eutectic mixture of glutamine and linoleic acid. That is, since the melting point of glutamine is known to be 185°C and the boiling point of linoleic acid is 230°C, the peak at 149.59°C is determined to be the eutectic point of a eutectic mixture of glutamine and linoleic acid, which is unrelated to the melting point of glutamine and the boiling point of linoleic acid.

[0098] [Experimental Example 8] Evaluation of Inhibition of Atopy and Inflammation Caused by Skin Irritation

[0099] Capsaicin was used as an effective stimulant to induce inflammatory cytokines that cause atopy or inflammation. Human-derived keratinocytes were cultured in a 6-well plate in a 5% CO2 incubator using DMEM medium (Addexbio). After treating with 50 micromoles (μmol) of capsaicin for 30 minutes to induce inflammatory cytokines that cause atopy and inflammation and directly affect cell death, skin wrinkle formation, and deterioration of elasticity, 1 ppm of capsazepine (CZ), which is effective in inhibiting capsaicin, was applied as a positive control for comparison. As a comparison group, a cosmetic composition for improving skin itching and atopy containing a eutectic mixture of glutamine and linoleic acid was applied at the same concentration as the groups treated with glutamine alone and linoleic acid alone, and compared. Subsequently, the concentration of suppressed inflammatory cytokines was quantified using an enzyme-linked immunosorbent assay (ELISA), and the results are shown in Figures 8 to 11. The experiment was evaluated by repeating it three times, and the results confirmed that all items were significant within a confidence interval of at least 95% (with a sample error of ±5%).

[0100] The types of inflammatory cytokines evaluated in this experiment and their roles in the skin's inflammatory response are as follows.

[0101] MCP-1: An inflammatory response initiator secreted by dermal cells (fibroblasts)

[0102] IL-1α: Secreted by keratinocytes as a factor involved in acute and chronic inflammation; along with TNF-α, it disrupts the skin barrier and promotes inflammation during the onset of atopic dermatitis.

[0103] IL-4: A key factor inducing type 2 inflammation (parasitic infection) and a core factor in atopic dermatitis causing chronic itching, increased risk of external infection, chronic inflammation, and barrier deterioration.

[0104] IL-6: A pro-inflammatory cytokine playing a key role in immune-mediated inflammatory diseases, and the most fundamental factor related to inflammation.

[0105] IL-8 / CXCL-8: Inflammatory mediators secreted by macrophages and epithelial cells

[0106] MMP-1: A proteolytic enzyme that acts specifically on collagen, inducing deterioration of skin elasticity and increased wrinkles (decrease in dermal density)

[0107] MMP-9: Decomposes gelatin and collagen, major components of the epidermal basement membrane, and is involved in the migration of inflammatory cells and tissue infiltration.

[0108] TNF-α: Tumor necrosis factor that mediates inflammatory responses, regulates cellular immune responses, and is involved in cell death. In atopic dermatitis, it is involved in inflammation and barrier deterioration.

[0109] According to Figure 8, MCP-1 showed a maximum 11% increase in inflammatory factors (50 ppm) when treated with glutamine alone and a 72.24% decrease in inflammatory factors (50 ppm) when treated with linoleic acid alone. On the other hand, when Example 1 was applied, a 93.68% decrease in inflammatory factors (50 ppm) was observed, confirming a significant increase in efficacy at the 29.7% level compared to treatment with linoleic acid alone.

[0110] According to Figure 8, IL-1α, which is involved in skin barrier disruption and inflammation promotion, was reduced by up to 71.82% (50 ppm) when treated with glutamine alone and up to 76.21% (50 ppm) when treated with linoleic acid alone, and when Example 1 was applied, the inflammatory factor was reduced by up to 82.89% (50 ppm), confirming an increase in efficacy of 8.8% compared to treatment with linoleic acid alone.

[0111] According to Figure 9, in the case of IL-4, an inflammatory cytokine known to be highly involved in the development of atopy, both glutamine alone and linoleic acid alone showed an inhibitory effect on inflammatory factors at a maximum level of 23.42% (50 ppm), but when Example 1 was applied, the maximum level was 43.40% (50 ppm), confirming a significant increase in efficacy at a level of 101.5% compared to glutamine alone and linoleic acid alone.

[0112] According to Figure 9, in the case of the pro-inflammatory cytokine IL-6, the inhibitory efficacy was confirmed to be up to 48.73% (50 ppm) when treated with glutamine alone and up to 53.22% (50 ppm) when treated with linoleic acid alone, and when Example 1 was applied, the inhibitory efficacy was up to 64.47% (50 ppm), confirming an increase in efficacy of 32.3% compared to treatment with glutamine alone and 21.1% compared to treatment with linoleic acid alone.

[0113] According to Figure 10, in the case of IL-8 / CXCL-8, treatment with glutamine alone at 50 ppm promoted cytokine secretion by 6.42%, and no inhibitory effect was observed at any treatment concentration. When linoleic acid was treated alone, an inhibitory effect of 13.85% was observed only in the 50 ppm treatment group. On the other hand, when Example 1 was applied, inhibitory effects were observed at both 5 ppm and 50 ppm, showing a maximum inhibitory effect of 38.97% (50 ppm). Consequently, a significant increase in efficacy of 181.4% was confirmed in the 50 ppm treatment group compared to treatment with linoleic acid alone.

[0114] According to Figure 10, TNF-α is a factor involved in inflammation mediation and apoptosis, showing a maximum inhibitory effect of 9.69% when treated with glutamine 0.5 ppm alone and an inhibitory effect of 0.87% when treated with glutamine 50 ppm alone. A maximum inhibitory effect of 30.06% was shown when treated with linoleic acid 50 ppm alone. When Example 1 was applied, the 50 ppm treatment group showed a maximum inhibitory effect of 46.86%, showing a significant improvement in efficacy of 383.5% compared to glutamine alone and 55.9% compared to linoleic acid alone based on maximum inhibitory efficacy.

[0115] According to Figure 11, MMP-1 is a collagen-degrading enzyme and is a key indicator for assessing skin elasticity and wrinkle increase. When treated with glutamine alone, it showed an inhibitory effect of up to 47.90% (50 ppm), and when treated with linoleic acid alone, it showed an inhibitory effect of up to 53.54% (50 ppm). When Example 1 was applied, the 50 ppm treatment group showed an inhibitory effect of up to 64.41%, confirming an improvement in efficacy of 34.5% and 20.3% compared to treatment with glutamine alone and treatment with linoleic acid alone, respectively.

[0116] According to Figure 11, MMP-9 is a gelatinizing enzyme that causes the breakdown of the epidermal basement membrane and is an indicator involved in the destruction of the skin barrier. When treated with glutamine alone, it showed an inhibitory effect of up to 18.78% (50 ppm), and when treated with linoleic acid alone, it showed an inhibitory effect of up to 30.31% (50 ppm). When Example 1 was applied, the 50 ppm treatment group showed an inhibitory effect of up to 47.58%, confirming a significant improvement in efficacy of 153.3% and 57% compared to treatment with glutamine alone and treatment with linoleic acid alone, respectively.

[0117] [Experimental Example 9] Evaluation of Antimicrobial Efficacy

[0118] The antibacterial efficacy against S. aureus, a representative harmful skin bacterium that exacerbates the skin barrier and is known to be prevalent in sensitive and atopic skin, particularly for causing atopic dermatitis, was evaluated. The antibacterial efficacy was evaluated using the disk diffusion assay. For the disk diffusion assay, a single bacterial colony was inoculated into 8 ml of trypsin-soy medium and incubated at 35°C for 24 hours. 0.1 ml of culture medium was spread onto pre-solidified trypsin-soy agar in a Petri dish. Subsequently, a sterilized paper disk was placed in the center of the Petri dish, and 0.02 ml of evaluation samples were dropped onto it to absorb: 1) glutamine 0.5%, 2) linoleic acid dispersed in 1,2-hexanediol 0.2% (same content as Example 1), and 3) the sample from Example 1 (0.2%). The samples were then incubated at 35°C for 24 hours. The width of the region was determined by measuring the diameter of the growth inhibition region after culture. The width of the region is determined as follows.

[0119] W=(TD) / 2

[0120] T: Total diameter of the growth inhibition zone (mm)

[0121] D: Sample diameter (mm)

[0122] W: Width of the growth inhibition zone (mm)

[0123] As a result, glutamine was found to have no antibacterial efficacy, and linoleic acid was found to have a growth inhibition zone diameter of 1.5 mm. In contrast, Example 1 was found to have a growth inhibition zone diameter of 3.5 mm, which was sufficiently permeable to the cell wall due to the high permeability of the eutectic mixture of glutamine and linoleic acid, and a 444% increase in antibacterial efficacy compared to linoleic acid (square of the diameter ratio as the ratio of the growth inhibition zone).

[0124] [Experimental Example 10] Erythema alleviation experiment

[0125] Cosmetic compositions for improving skin itching and atopic dermatitis, comprising glutamine, linoleic acid, and a eutectic mixture of glutamine and linoleic acid, were applied at a concentration of 1% to a site on the forearm treated with 0.1% capsaicin, respectively, and the anti-inflammatory effect was confirmed after 24 hours. As a result, it was confirmed that the erythema induced by capsaicin was most significantly alleviated and reduced in the composition of the present invention.

[0126] [Experimental Example 11] Measurement of Transepidermal Water Loss (TEWL)

[0127] To confirm the effect of improving the skin barrier, transepidermal water loss (TEWL) was measured. TEWL was measured using the Tewameter® TM300 (Courage + Khazaka electronic GmbH, Germany). This measuring instrument is based on the diffusion principle of an open chamber, and the temperature and humidity sensors of the probe measure the amount of water evaporation per unit area (g / m2 / h) of the measurement site over time. In this experiment, the test site was analyzed after measurement, and the average of the last three values ​​was analyzed during a stabilization period of 10 to 60 seconds. The evaluation was performed on the forearms of a total of 15 subjects. The evaluation samples were conducted using a cosmetic composition for improving skin itching and atopic dermatitis (Example 1, glutamine 0.5%, linoleic acid 0.2%) comprising 1) glutamine (0.7%), 2) linoleic acid (0.7%), 3) a simple mixture of glutamine and linoleic acid (Comparative Example 2, glutamine 0.5%, linoleic acid 0.2%), and 4) a eutectic mixture of glutamine and linoleic acid. As a result, as shown in Table 3 above, when Example 1 was applied after 2 weeks, a 19.8% reduction in transepidermal water loss (TEWL) was observed, confirming that the skin barrier was improved by the cosmetic composition for improving skin itching and atopic dermatitis containing a eutectic mixture of glutamine and linoleic acid according to the present invention.

[0128] Period Glutamine Linoleic Acid Simple mixture of glutamine and linoleic acid Eutectic mixture of glutamine and linoleic acid After 1 day 1.5% improvement 2.5% improvement 2.7% improvement 6.5% improvement After 1 week 3.6% improvement 3.3% improvement 9.5% improvement 12.0% improvement After 2 weeks gn 9.5% improvement 6.5% improvement 15.5% improvement 19.8% improvement

[0129] [Example 3] Skin toner formulation

[0130] Composition Ratio (%) Example 1 10.00 Purified Water 75.28 Acrylate / C10-30 Acrylate Crosspolymer 0.10 Xanthan Gum 0.10 Trisodium EDTA 0.02 Propanediol 6.00 Glycerin 4.00 Panthenol 2.00 1,2-Hexanediol 2.00 Tromethamine 0.10 Polyacrylate Crosspolymer 60.40

[0131] The skin toner formulation of Example 3 can be prepared by including 10% of Example 1. It can be prepared by mixing all the ingredients in the table above (11 components of Example 1 - Polyacrylate Crosspolymer-6) and stirring (dispersing) at 2000 rpm for 20 minutes, and the method of preparing the skin toner formulation is not limited to the described method. The skin toner formulation of Example 3 containing Example 1 aesthetically improves skin texture, and through the skin toner formulation of Example 3, one can experience improved skin elasticity and a soothing sensation on the skin after outdoor activities.

[0132] [Example 4] Lotion formulation

[0133] Composition Ratio (%) Example 1 10.00 Purified Water 60.12 Carbomer 0.10 PEG / PPG / Polybutylene Glycol-8 / 5 / 3 Glycerin 1.50 Trisodium EDTA 0.04 Urea 1.85 Hydroxyethylpiperazine Ethanesulfonic Acid 1.85 Trehalose 2.50 Panthenol 1.35 2,3-Butanediol 5.00 1,2-Hexanediol 1.50 PEG / PPG-17 / 6 Copolymer 1.0 0 Glycereth-26 1.22 Tromethamine 0.10 Polyacrylate Crosspolymer-60 14 Caprylic Methicone 1.23 Squalane 2.50 Caprylic / Capric Triglyceride 1.50 Dimethicone 2.20 PCA Dimethicone 2.50 Dimethiconol 0.60 Silica 0.10 Sorbitan Oliveate 0.30 Cetyl Malmitate 0.30 Glyceryl Stearate 0.50

[0134] The lotion formulation of Example 4 can be prepared by including 10% of Example 1. The water-soluble components and polymer components (14 components of purified water - polyacrylate crosspolymer-6) of the table above are stirred at 2000 rpm for 20 minutes at 40°C. Meanwhile, the oil-soluble components (10 components of caprylic methicone - glyceryl stearate) of the table above are stirred separately at 2000 rpm for 20 minutes at 40°C, and then the stirred oil-soluble components (10 components of caprylic methicone - glyceryl stearate) of the table are added to the stirred water-soluble components and polymer components (14 components of purified water - polyacrylate crosspolymer-6) of the table, and emulsified using a homogenizer at 5000 rpm for 20 minutes at 40°C. Afterward, the lotion formulation can be prepared by adding Example 1 after cooling to room temperature, and the method of preparing the lotion formulation is not limited to the described method. The lotion formulation of Example 4 containing Example 1 aesthetically improved skin texture, and through the lotion formulation of Example 4, improvement in skin elasticity and a soothing sensation on the skin after outdoor activities could be felt, and after using the lotion formulation of Example 4 containing Example 1 for 2 weeks, the effects of pore reduction and improvement in skin texture could be confirmed.

[0135] [Example 5] Cream formulation

[0136] Composition Ratio (%) Example 1 10.00 Purified Water 57.68 Trisodium EDTA 0.02 Panthenol 1.00 Propanediol 2.00 Glycerin 5.00 1,2-Hexanediol 2.00 Carbomer 0.15 Acrylate / C10-20 Alkyl Acrylate Crosspolymer 0.15 Tromethamine 0.30 Caprylic / Capric Triglyceride 5.00 Squalane 4.00 Isostearyl Isostearate 4.00 Dimethicone 2.50 Shea Butter 1.00 Hydrogenated Polyisobutene 4.00 Cetearyl Alcohol 0.80 PEG-40 Stearate 0.40

[0137] The cream formulation of Example 5 can be prepared by including 10% of Example 1. The water-soluble components and polymeric components (9 components of purified water and tromethamine) of the table above are stirred at 2000 rpm for 20 minutes at 40°C. Meanwhile, the oil-soluble components (8 components of caprylic / capric triglyceride and PEG-40 stearate) of the table above are stirred separately at 2000 rpm for 20 minutes at 40°C, and then the stirred oil-soluble components (8 components of caprylic / capric triglyceride and PEG-40 stearate) of the table above are added to the stirred water-soluble components and polymeric components (9 components of purified water and tromethamine) of the table above, and emulsified using a homogenizer at 5000 rpm for 20 minutes at 40°C. Afterward, the cream formulation can be prepared by cooling to room temperature and adding Example 1, and the method of preparing the cream formulation is not limited to the described method. The cream formulation of Example 5 containing Example 1 aesthetically improves skin texture and has excellent moisturizing properties. Through the cream formulation of Example 5, one could feel immediate skin elasticity and feel comfort in the skin the next day after waking up. Furthermore, one could feel improvement in skin elasticity and soothing of the skin after outdoor activities, and after using the cream formulation of Example 5 containing Example 1 for 2 weeks, effects of pore reduction, improvement in skin texture, and improvement in micro-wounds and skin irregularities were confirmed.

[0138] [Example 5] Clinical trial using a cream formulation

[0139] 1) Evaluation of improvement in itching due to restoration of barrier function

[0140] ① Evaluator: The transepidermal water loss at the test site, measured by a transepidermal water loss measuring device, is 12 g / m² 2 12 adult women aged 20–65 (47.08 ± 11.12 years) corresponding to / h or more

[0141] ② Application method: For 2 weeks, twice a day in the morning and evening, after cleansing, apply [[Example 5] cream formulation + soybean panthenol cream] and [soybean panthenol cream] to the area 3 cm below the popliteal fossa on both arms, respectively, and allow them to absorb. Also, prepare a case without application.

[0142] ③ Evaluation Method:

[0143] ⓐ Evaluation of Itch Scale Improvement (Subject Subjective Evaluation)

[0144] ⓑ Evaluation of improvement in transepidermal water loss using Tewameter TM300, and evaluation of improvement in skin redness using a spectrophotometer and digital camera

[0145] ⓒ Satisfaction evaluation through subject surveys

[0146] ⓓ Visual evaluation by a dermatologist based on the ESIF scale

[0147] Item [Example 5] Cream Formulation + Soybean Panthenol Cream Application Soybean Panthenol Cream Alone Application No Application Evaluation of Itch Improvement Itch decreased by 44.94% Itch decreased by 29.21% Itch decreased by 16.05% Evaluation of Maximum Itch Improvement Maximum itch decreased by 64.00% Maximum itch decreased by 43.00% Maximum itch decreased by 8.89% Degree of Barrier Improvement Barrier decreased by 14.06% Barrier decreased by 7.02% Barrier increased by 1.99% Degree of Redness Improvement Redness decreased by 11.67% Redness decreased by 6.90% Redness decreased by 0.89%

[0148] [Example 5] The evaluator's perceived satisfaction with the cream formulation + application of Soy Panthenol Cream was 4.58 / 5.00 points, compared to the evaluator's perceived satisfaction with the application of Soy Panthenol Cream alone (1.92 / 5.00 points), indicating that the evaluator felt a definite improvement in itching.2) Evaluation of skin sensitivity alleviation

[0149] ① Evaluators: Adult men and women aged 20 years or older with sensitive skin selected via the lactic acid stinging test (51.50 ± 10.54 years)

[0150] ② Application method: For 2 weeks, twice a day in the morning and evening, apply [[Example 5] cream formulation + soybean panthenol cream] and [soybean panthenol cream] to each side of the face and allow them to absorb, and also prepare a case without application.

[0151] ③ Evaluation Method:

[0152] ⓐ Evaluation of skin redness improvement using the VISIA-CR pre-eye imaging system and image analysis program

[0153] ⓑ Evaluation of improvement in transepidermal water loss by Tewameter TM300

[0154] ⓒ Satisfaction evaluation through subject surveys

[0155] ⓓ Visual evaluation by a dermatologist based on the ESIF scale

[0156] Item [Example 5] Cream formulation + Soybean Panthenol Cream application Soybean Panthenol Cream alone application No application Degree of redness improvement Redness 6.08% decrease Redness 3.01% decrease Redness 1.92% decrease Degree of skin barrier improvement Skin barrier 10.45% decrease Skin barrier 6.53% decrease Skin barrier 1.31% decrease

[0157] Dermatologist's Facial Skin ESIF Evaluation Results [Example 5] Cream Formulation + Soy Panthenol Cream Application Soy Panthenol Cream Alone Application No Application Before Use 2 Weeks After Use Before Use 2 Weeks After Use Before Use 2 Weeks After Use Mean 1.80 1.10 1.80 1.30 2.00 1.73 Standard Deviation 0.63 0.32 0.63 0.48 0.45 0.47

[0158] Dermatologist's judgment criteria E (erythema, inflammation), S (scaling), I (induration), F (cracks) Comprehensive evaluation criteria [Example 5] Verification of the excellence of the cream formulation + application of medicinal soy panthenol cream [Example 5] The evaluator's perceived satisfaction with the cream formulation + application of medicinal soy panthenol cream was 4.80 / 5.00 points, compared to the evaluator's perceived satisfaction with the application of medicinal soy panthenol cream alone (4.20 / 5.00 points), indicating that the evaluator perceived skin improvement. Through this experiment, it can be expected that the cream formulation of [Example 5] will have definite efficacy as a soothing ingredient for sensitive skin, and it can be certainly expected to be effective in alleviating atopic dermatitis along with an inhibitory effect on related inflammatory factors.

[0159] As described above, the cosmetic composition for improving skin itching and atopic dermatitis according to the present invention, the cosmetic product containing the same, and the method for manufacturing the same include a eutectic mixture of glutamine and linoleic acid to increase the penetration of glutamine through the skin and enhance the solubility of linoleic acid in water, and have a complex synergistic effect in improving atopic symptoms and promoting skin regeneration by including a eutectic mixture of glutamine and linoleic acid.

[0160] As described above, the present invention has been explained with reference to various embodiments, experimental examples, and comparative examples, but this is merely illustrative, and it should be understood that various modifications and equivalent alternative embodiments are possible based on the ordinary knowledge of the field to which the technology belongs. Accordingly, the true technical scope of protection of the present invention is defined by the claims described below and should be determined based on the specific details of the invention described above.

[0161] The present invention relates to a cosmetic composition for improving skin itching and atopic dermatitis, a cosmetic product containing the same, and a method for manufacturing the same, and is applicable to industrial fields related to cosmetic manufacturing.

Claims

1. A cosmetic composition for improving skin itching and atopic dermatitis comprising a eutectic mixture of glutamine and linoleic acid.

2. In Paragraph 1, The cosmetic composition for improving skin itching and atopic dermatitis is characterized by additionally including water and a linear diol having 5 to 8 carbon atoms, having hydroxyl groups at the 1st and 2nd carbon atoms and not having a branched structure.

3. In Paragraph 2, A cosmetic composition for improving skin itching and atopic dermatitis, characterized in that the linear diol is 1,2-hexanediol.

4. In Paragraph 3, A cosmetic composition for improving skin itching and atopic dermatitis, characterized in that the glutamine is 2-6 parts by weight, the linoleic acid is 0.4-4 parts by weight, the 1,2-hexanediol is 10-20 parts by weight, and the water is 70-87.6 parts by weight.

5. In Paragraph 3, A cosmetic composition for improving skin itching and atopic dermatitis, characterized by additionally including quercetin in the above cosmetic composition for improving skin itching and atopic dermatitis.

6. In Paragraph 5, A cosmetic composition for improving skin itching and atopic dermatitis, characterized by additionally including ethyl alcohol and dipropylene glycol.

7. In Paragraph 6, A cosmetic composition for improving skin itching and atopic dermatitis, characterized in that the glutamine is 2-6 parts by weight, the linoleic acid is 0.4-4 parts by weight, the 1,2-hexanediol is 10-20 parts by weight, the water is 65.97-83.63 parts by weight, the quercetin is 0.01-0.03 parts by weight, the ethyl alcohol is 2.61-2.63 parts by weight, and the dipropylene glycol is 1.35-1.37 parts by weight.

8. A cosmetic for improving skin itching and atopic dermatitis comprising the cosmetic composition for improving skin itching and atopic dermatitis according to claims 1 to 7.

9. A step in which 2-6 parts by weight of glutamine and 65.97-83.63 parts by weight of water are heated to 40℃-90℃ and stirred at 1,300 rpm-2,000 rpm to produce a first solution in which glutamine is dissolved in water; A step of mixing 0.4-4 parts by weight of linoleic acid and 10-20 parts by weight of 1,2-hexanediol at room temperature at 500 rpm - 1,000 rpm to produce a second solution in which linoleic acid is dissolved in 1,2-hexanediol; and A method for preparing a cosmetic composition for improving skin itching and atopic dermatitis, comprising the step of: introducing the second solution into the first solution while heating it to 40°C-90°C and stirring at 3,000 rpm-6,000 rpm, or introducing the second solution into the first solution while pressurizing it to 4,000 psi-35,000 psi and dispersing it to produce a eutectic mixture of glutamine and linoleic acid.

10. A step in which 2-6 parts by weight of glutamine and 70-87.6 parts by weight of water are heated to 40℃-90℃ and stirred at 1,300 rpm-2,000 rpm to produce a first-1 solution in which glutamine is dissolved in water; A step in which 0.4-4 parts by weight of linoleic acid and 10-20 parts by weight of 1,2-hexanediol are stirred at room temperature at 500 rpm - 1,000 rpm to produce a 2-1 solution in which linoleic acid is dissolved in 1,2-hexanediol; A step of producing a third solution by mixing 0.01-0.03 parts by weight of quercetin, 2.61-2.63 parts by weight of ethyl alcohol, and 1.35-1.37 parts by weight of dipropylene glycol; and A method for preparing a cosmetic composition for improving skin itching and atopic dermatitis, comprising the step of: introducing the above-mentioned 2-1 solution into the above-mentioned 1-1 solution while heating to 40℃-90℃ and stirring at 3,000rpm-6,000rpm while introducing the above-mentioned 3 solution in between; or introducing the above-mentioned 2-1 solution into the above-mentioned 1-1 solution while pressurizing and dispersing at 4,000psi-35,000psi and introducing the above-mentioned 3 solution in between, thereby producing a eutectic mixture of glutamine and linoleic acid supported with quercetin.

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