Composition having effect of Anti-redness and use thereof

Abstract

Disclosed in the present invention are a composition having an effect of anti-redness and use thereof. The composition comprises the following components by mass percentage: 90% to 99% of a lipid-soluble solvent and 1% to 10% of a saururus chinensis extract. The lipid-soluble solvent is selected from at least one of hexylene glycol, caprylic / capric triglyceride, sunflower seed oil, macadamia nut oil, meadowfoam seed oil, silicone oil, squalane, ethylhexyl palmitate, diisostearyl malate, polyglyceryl-2 triisostearate, linseed seed oil, octyldodecanol, and coco-caprylate / caprate. In the present invention, the composition prepared by mixing the saururus chinensis extract with the lipid-soluble solvent has a very significant effect of anti-redness.

Description

A composition with anti - redness effect and its application Technical Field

[0001] The present invention relates to the technical field of cosmetics, and particularly to a composition with anti - redness effect and its application. Background Art

[0002] In recent years, skin problems related to sensitive skin, such as skin burning, itching, erythema, and damaged skin barrier, have increasingly attracted attention. These skin discomfort symptoms are generally induced by endogenous or exogenous factors, and their manifestations of flushing and burning are the most prominent. The underlying mechanism is closely related to high vascular reactivity. Among them, telangiectasia of facial capillaries causing telangiectasia not only seriously affects the beauty of patients, but also causes a great burden on the patients' psychology. With the continuous improvement of social development and people's living standards, the requirements for the quality and performance of cosmetics are also getting higher and higher. People hope to have a series of functional cosmetics that are harmless to human health and have beauty and health care functions.

[0003] Using Chinese herbal medicine extracts as additives in beauty and skin - care cosmetics has the characteristics of stable and lasting efficacy, mild action on the skin, low irritation, high safety, and remarkable curative effect. At present, most of the liposoluble cosmetic raw materials with anti - redness effects in the market are mainly based on anti - inflammatory models. Such raw materials not only have a large addition amount, but also have a slow onset time and cannot quickly solve the problem of skin redness. Moreover, water - soluble and alcohol - soluble cosmetic raw materials are difficult to be compatible with essential oil products, makeup remover oils, and color cosmetics.

[0004] Saururus chinensis is sweet, pungent, and cold in nature. According to the "Tang Materia Medica" record: "It is mainly used for treating edema, beriberi, promoting urination and defecation, eliminating phlegm and breaking stagnation, removing accumulations, and treating furuncles and carbuncles". It has the effects of diuresis and detumescence, clearing heat and detoxifying, and can be used to treat symptoms such as edema, dysuria, and stranguria with pain. Saururus chinensis contains a variety of chemical components, mainly lignans, flavonoids, volatile oils, alkaloids, polysaccharides, polyphenols, liposoluble terpene components, etc. Summary of the Invention

[0005] Aiming at the deficiencies of the prior art, the purpose of the present invention is to develop a cosmetic raw material that can be compatible with essential oil products, makeup remover oils, and color cosmetics, can quickly and effectively improve skin redness, and has the effect of repairing the skin barrier. Specifically, the purpose of the present invention is to provide a composition with anti - redness effect and its application. The present invention uses a liposoluble extract of Saururus chinensis and dissolves it in a specific liposoluble solvent to obtain a composition with a significant anti - redness effect.

[0006] The purpose of the present invention is achieved by the following technical solutions:

[0007] In a first aspect, the present invention provides a composition having redness-reducing effects, comprising the following components in weight percentages: 90-99% fat-soluble solvent and 1-10% *Saururus chinensis* extract;

[0008] The fat-soluble solvent is selected from at least one of the following: acetodiol, caprylic / capric triglyceride, sunflower seed oil, macadamia nut oil, meadowfoam seed oil, silicone oil, squalane, ethylhexyl palmitate, diisostearyl malate, polyglycerol-2 triisostearyl, linseed oil, octyldodecanool, and cocoyl octanoate / capric ester.

[0009] As a preferred embodiment, the composition comprises the following components in the following mass percentages: 90-97% fat-soluble solvent and 3-10% *Saururus chinensis* extract. More preferably, the composition comprises the following components in the following mass percentages: 90-95% fat-soluble solvent and 3-5% *Saururus chinensis* extract.

[0010] As a preferred embodiment, the lipid-soluble solvent is selected from at least one of the following: glycol, caprylic / capric triglyceride, sunflower seed oil, macadamia nut oil, meadowfoam seed oil, ethylhexyl palmitate, diisostearyl malate, polyglycerol-2-triisostearyl, linseed oil, and octyldodecanoate. In the inventors' previous experimental studies, it was found that the type of lipid-soluble solvent used has a significant impact on the solubility and stability of the prepared composition, thus affecting its redness-reducing efficacy. For example, when silicone oil, squalane, or coconut oil-caprylic / capric triglyceride is used as the lipid-soluble solvent, the solubility and stability of the prepared composition are poor.

[0011] As a further preferred embodiment, the fat-soluble solvent is selected from at least one of caprylic / capric triglyceride, macadamia nut oil, and octyldodecanool, and the composition prepared therefrom has the best redness-reducing effect.

[0012] As a preferred embodiment, the *Saururus chinensis* extract is a fat-soluble *Saururus chinensis* extract.

[0013] As a preferred embodiment, the *Saururus chinensis* extract is prepared by the following method:

[0014] The raw material of *Sanbaicao* is extracted with alcohol, then filtered, and then subjected to resin adsorption and alcohol desorption, followed by concentration and drying to obtain the final product.

[0015] As a preferred embodiment, the alcohol is selected from at least one of ethanol, propanol, butanol, glycerol, and butanediol.

[0016] As a preferred embodiment, the resin is a macroporous resin.

[0017] As a preferred embodiment, the process of eluting with alcohol further includes a decolorization step.

[0018] As a preferred embodiment, the preparation method of the *Trifolium repens* extract includes the following steps:

[0019] A1. Extraction: After cutting the raw material of Sanbaicao into sections, it is extracted by reflux with ethanol at a volume fraction of 60-70%;

[0020] A2. Enrichment: Filter the extract obtained in step A1, load the filtrate a onto a macroporous resin, and then desorb it using 80-95% ethanol by volume. Collect the desorbed liquid, concentrate and dry it to obtain the *Trifolium repens* extract.

[0021] As a preferred embodiment, in step A1, the reflux extraction temperature is 80-90℃, the extraction is performed 2-3 times, and the extraction time for each extraction is 1.0-2.0h.

[0022] As a preferred embodiment, in step A1, the amount of ethanol used is 6 to 12 Bv.

[0023] As a preferred embodiment, in step A2, the macroporous resin is selected from at least one of D101, D101C, LX-100B, LX-T28, LX-T81, AB-8, and DM130.

[0024] As a preferred embodiment, in step A2, the volume-to-mass ratio of the macroporous resin to the *Trichosanthes kirilowii* raw material is 0.5–2 (ml):1 (g); the flow rate of filtrate a is 0.5–3 Bv / h.

[0025] As a preferred embodiment, in step A2, the amount of ethanol with a volume fraction of 80-95% is 1-6 times the volume of the macroporous resin, and the flow rate is 0.5-3 Bv / h.

[0026] As a further preferred embodiment, in step A2, the ethanol used for desorption is ethanol with a volume fraction of 90-95%, and most preferably ethanol with a volume fraction of 90%.

[0027] As a preferred embodiment, in step A2, the concentration and drying process uses a vacuum degree of 0.01–0.1 MPa and a temperature of 50–70°C.

[0028] In the inventors' preliminary experimental studies, it was found that the *Saururus chinensis* extract obtained using the aforementioned extraction and enrichment steps could further improve the redness-reducing effect of the composition. Specifically, in step A2, using ethanol with a volume fraction of 80-95% and an amount 1-6 times the volume of the macroporous resin for desorption resulted in a better redness-reducing effect when the obtained *Saururus chinensis* extract was used in the composition. If less than 80% ethanol was used for desorption, or if the amount of 80% ethanol was too low, the redness-reducing effect of the resulting composition decreased to varying degrees.

[0029] As a further preferred embodiment, in order to remove the pigments from the Saussurea costatum extract and lighten the color of the Saussurea costatum extract so that the prepared composition can be practically applied in cosmetic products, in step A2, the collected desorbed liquid also includes a decolorization step before concentration and drying.

[0030] The decolorization process is as follows: the desorbed liquid is first decolorized with activated carbon and then filtered. The resulting filtrate b is then decolorized with anionic macroporous resin. The decolorized liquid is collected and the pH is adjusted to 6.5-7.1.

[0031] As a preferred embodiment, in the decolorization step, the activated carbon is coconut shell activated carbon with a mesh size of 200-300; the amount of activated carbon used is 0.1-1% of the mass of the *Saururus chinensis* raw material. Using activated carbon can remove impurities such as chlorophyll from the desorption solution, thereby improving the stability of the *Saururus chinensis* extract and making the color lighter.

[0032] As a preferred embodiment, in the decolorization step, the temperature for decolorization with activated carbon is 15–60°C, and the stirring time is 20–60 min.

[0033] As a preferred embodiment, in the decolorization step, the filtration is performed using a 0.22–0.8 μm membrane.

[0034] As a preferred embodiment, in the decolorization step, the anionic macroporous resin is a weak anionic macroporous resin, specifically selected from at least one of LX-T5, LX-94, D941, LXD-762, LXB-AS, and LXB-400. Using anionic macroporous resin can remove the red pigment impurities from the filtrate b.

[0035] As a preferred embodiment, in the decolorization step, when using anionic macroporous resin for decolorization, the volume ratio of filtrate b to anionic macroporous resin is 10-30:1, and the flow rate of filtrate b is 0.5-3 Bv / h. Excessive use of anionic macroporous resin will lead to a decrease in the red-fading effect of the final prepared composition; conversely, insufficient use will result in a decrease in the decolorization effect. In the inventors' previous experimental research, it was found that only by using activated carbon decolorization followed by anionic macroporous resin decolorization can the pigment impurities in the *Saussurea involucrata* extract be effectively removed, allowing the prepared composition to achieve the required color; simultaneously, the red-fading effect of the prepared composition is improved. If only activated carbon decolorization or anionic macroporous resin decolorization is performed, or if anionic macroporous resin decolorization is used first followed by activated carbon decolorization, the pigment removal effect in the *Saussurea involucrata* extract will decrease to varying degrees, resulting in varying degrees of darkening of the prepared composition's color, and a certain reduction in the red-fading effect of the composition.

[0036] Secondly, the present invention provides the use of the aforementioned composition in the preparation of cosmetics with redness-reducing effects.

[0037] As a preferred embodiment, in the cosmetic product with redness-reducing effect, the aforementioned composition is added at a mass percentage of 0.01 to 10%.

[0038] Compared with the prior art, the present invention has the following beneficial effects:

[0039] 1) The composition prepared by mixing the extract of Saururus chinensis with a fat-soluble solvent has a very significant redness-reducing effect.

[0040] 2) The present invention can further improve the redness-reducing effect of the composition by specifically selecting the mixing ratio of the extract of Saussurea involucrata and the fat-soluble solvent, and by specifically selecting the type of fat-soluble solvent.

[0041] 3) The present invention can further improve the redness-reducing effect of the composition by using the extract of Saussurea costatum prepared by a specific process. Attached Figure Description

[0042] Other features, objects, and advantages of the present invention will become more apparent from the following detailed description of non-limiting embodiments with reference to the accompanying drawings:

[0043] Figure 1 shows photographs of the desorption solution after enrichment, the filtrate after decolorization with activated carbon, and the eluent after decolorization with anion exchange resin in Example 2 of the present invention.

[0044] Figure 2 shows the results of the alleviating effect of the test product 0.5% Y1 on skin redness induced by capsaicin, histamine, and methyl nicotinic acid;

[0045] Figure 3 shows the results of the alleviating effect of the test product 0.5% Y2 on skin redness induced by capsaicin, histamine, and methyl nicotinic acid;

[0046] Figure 4 shows a case study of the alleviating effect of the test product 0.5% Y2 on skin redness induced by capsaicin, histamine, and methyl nicotinic acid.

[0047] Figure 5 shows the results of the alleviating effect of the test product 0.5% Y3 on skin redness induced by capsaicin, histamine, and methyl nicotinic acid;

[0048] Figure 6 shows the results of the alleviating effect of the test product 0.5% Y4 on skin redness induced by capsaicin, histamine, and methyl nicotinic acid;

[0049] Figure 7 shows the results of the alleviating effect of the test product 0.5% Y5 on skin redness induced by capsaicin, histamine, and methyl nicotinic acid;

[0050] Figure 8 shows the results of the alleviating effect of the test product 0.5% Y6 on skin redness induced by capsaicin, histamine, and methyl nicotinic acid;

[0051] Figure 9 shows the results of the alleviating effect of the test product 0.5% Y7 on skin redness induced by capsaicin, histamine, and methyl nicotinic acid.

[0052] Figure 10 shows the results of the alleviating effect of the test product 0.5% Y8 on skin redness induced by capsaicin, histamine, and methyl nicotinic acid;

[0053] Figure 11 shows the results of the alleviating effect of the test product 0.5% Y9 on skin redness induced by capsaicin, histamine, and methyl nicotinic acid;

[0054] Figure 12 shows the results of the alleviating effect of the test product 0.5% Y10 on skin redness induced by capsaicin, histamine, and methyl nicotinic acid;

[0055] Figure 13 shows the results of the alleviating effect of the test product 0.5% Y11 on skin redness induced by capsaicin, histamine, and methyl nicotinic acid;

[0056] Figure 14 shows the results of the effect of the test product 0.5% Y12 on alleviating skin redness induced by capsaicin, histamine, and methyl nicotinic acid.

[0057] Figure 15 shows the results of the alleviating effect of the test product 0.5% Y13 on skin redness induced by capsaicin, histamine, and methyl nicotinic acid.

[0058] Figure 16 shows the results of the effect of the test product 0.5% Y14 on alleviating skin redness induced by capsaicin, histamine, and methyl nicotinic acid.

[0059] Figure 17 shows the results of the alleviating effect of the test product 0.5% Y16 on skin redness induced by capsaicin, histamine, and methyl nicotinic acid. Detailed Implementation

[0060] The present invention will now be described in detail with reference to specific embodiments. These embodiments will help those skilled in the art to further understand the present invention, but do not limit the invention in any way. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention. These all fall within the scope of protection of the present invention.

[0061] All Chinese herbal raw materials and reagents used in the experiments of this invention were provided by our company and / or purchased commercially. In the following examples, the percentage of ethanol used is a volume fraction. The content of triscutellarin in the *Saururus chinensis* raw material used was 0.14%, the total lignan content was 0.3%, and the total terpenoid content was 0.36%. Determination methods: Triscutellarin was determined according to the 2020 edition of the *Chinese Pharmacopoeia*; total terpenoids were determined according to the total triterpenoid content determination method for health food; and total lignans were determined using ultraviolet-visible spectrophotometry.

[0062] Example 1

[0063] This embodiment provides a composition comprising 3.5% by weight of *Saururus chinensis* extract and 96.5% by weight of caprylic / capric triglyceride (sample number Y1). The specific preparation steps of the composition are as follows:

[0064] 1. Extraction: Cut the raw material of Saururus chinensis (stems and leaves) into sections, add 500.0g of raw material, add 5000mL of 65% ethanol each time, reflux at 80-90℃ for 1.5h, extract twice, combine the extracts, cool to room temperature, and filter the extract;

[0065] 2. Enrichment: The filtrate obtained in the extraction step was loaded into 500 mL of D101 macroporous resin at a flow rate of 2.0 Bv / h; after loading, it was desorbed with 1500 mL of 80% ethanol at a flow rate of 1.0 Bv / h, and the desorbed solution was collected.

[0066] 3. Decolorization: Add 2.0g of 200-300 mesh coconut shell activated carbon to the eluent and stir at room temperature for 30min for decolorization. Then filter using a 0.45um membrane. The resulting filtrate is then loaded onto an LX-T5 anion exchange resin (the volume ratio of filtrate to LX-T5 anion exchange resin is 30:1) for decolorization. The column flow rate is 2.0 Bv / h. Collect the eluent and adjust the pH to 6.9-7.1 with citric acid. The solution before and after decolorization is shown in Figure 1.

[0067] 4. Concentration and Drying: The eluent obtained from the decolorization step was concentrated and dried under vacuum at a vacuum degree of 0.09 MPa, a drying temperature of 60℃, and a drying time of 10 hours. 4.0 g of extract (i.e., *Saururus chinensis* extract) was obtained, with a saururin content of 8.4% (yield: 48.0%), a total lignan content of 17% (yield: 45.3%), and a total terpene content of 20.0% (yield: 44.4%).

[0068] 5. Dissolution with fat-soluble solvents: Take 2.0g of the extract and add caprylic / capric triglyceride at a concentration of 3.5%. After dissolution and filtration, a stable composition is obtained.

[0069] Example 2

[0070] This embodiment provides a composition comprising 3.5% by weight of *Saururus chinensis* extract and 96.5% by weight of caprylic / capric triglyceride (sample number Y2). The specific preparation steps of the composition are as follows:

[0071] 1. Extraction: Cut the raw material of Saururus chinensis (stems and leaves) into sections, add 500.0g of raw material, add 5000mL of 70% ethanol each time, reflux at 80-90℃ for 1.5h, extract twice, combine the extracts, cool to room temperature, and filter the extract;

[0072] 2. Enrichment: The filtrate obtained in the extraction step was loaded onto 400 mL of D101 macroporous resin at a flow rate of 2.5 Bv / h; after loading, it was desorbed with 1500 mL of 90% ethanol at a flow rate of 1.0 Bv / h, and the eluent was collected.

[0073] 3. Decolorization: Add 2.0g of 200-300 mesh coconut shell activated carbon to the eluent, stir at room temperature for 30min for decolorization, and then filter through a 0.45um membrane; the resulting filtrate is then loaded onto LX-T5 anion exchange resin (the volume ratio of filtrate to LX-T5 anion exchange resin is 30:1) for decolorization, the column flow rate is 2.0 Bv / h, the eluent is collected and the pH is adjusted to 6.9-7.1 with citric acid;

[0074] 4. Concentration and Drying: The eluent obtained from the decolorization step was concentrated and dried under vacuum at a vacuum degree of 0.09 MPa, a drying temperature of 60℃, and a drying time of 10 h. 4.5 g of extract (i.e., *Saururus chinensis* extract) was obtained. HPLC analysis revealed the following contents: *Saururus chinensis* ketone content: 14%, yield: 66.8%; total lignan content: 28%, yield: 66.0%; total terpenoid content: 25.0%, yield: 32%.

[0075] 5. Dissolution with fat-soluble solvents: Take 1.0g of extract and add caprylic / capric triglyceride at a concentration of 3.5%. After dissolution and filtration, a stable composition is obtained.

[0076] Example 3

[0077] This embodiment provides a composition comprising 3.5% by weight of *Saururus chinensis* extract and 96.5% by weight of caprylic / capric triglyceride (sample number Y3). The specific preparation steps of the composition are as follows:

[0078] 1. Extraction: Cut the raw material of Saururus chinensis (stems and leaves) into sections, add 500.0g of raw material, add 5000mL of 70% ethanol each time, reflux at 80-90℃ for 1.5h, extract twice, combine the extracts, cool to room temperature, and filter the extract;

[0079] 2. Enrichment: The filtrate obtained from the extraction step was loaded onto 400 mL of D101 macroporous resin at a flow rate of 2.5 Bv / h. After loading, the solution was desorbed with 1500 mL of 95% ethanol at a flow rate of 1.0 Bv / h, and the desorbed solution was collected.

[0080] 3. Decolorization: Add 2.0g of 200-300 mesh coconut shell activated carbon to the eluent and stir at room temperature for 30min for decolorization. Then filter through a 0.45um membrane. The resulting filtrate is then loaded onto an LX-T5 anion exchange resin (the volume ratio of filtrate to LX-T5 anion exchange resin is 30:1) for decolorization. The flow rate is 2.0 Bv / h. Collect the eluent and adjust the pH to 6.9-7.1 with citric acid.

[0081] 4. Concentration and Drying: The eluent obtained from the decolorization step was concentrated and dried under vacuum at a vacuum degree of 0.09 MPa, a drying temperature of 60℃, and a drying time of 10 h. 4.8 g of extract (i.e., *Saururus chinensis* extract) was obtained. HPLC analysis revealed the following contents: *Saururus chinensis* ketone content: 10.2% (yield: 69.9%); total lignan content: 23% (yield: 73.6%); and total terpenoid content: 25.5% (yield: 68.0%).

[0082] 5. Dissolution with fat-soluble solvents: Take 1.0g of extract and add caprylic / capric triglyceride at a concentration of 3.5%. After dissolution and filtration, a stable composition is obtained.

[0083] Example 4

[0084] This embodiment provides a composition comprising 3.5% by weight of *Saussurea costatum* extract and 96.5% by weight of macadamia nut oil (sample number Y4). The specific preparation steps of the composition are basically the same as those in Example 2, except that caprylic / capric triglycerides in step 5 are replaced with macadamia nut oil.

[0085] Example 5

[0086] This embodiment provides a composition comprising 3.5% by weight of *Saururus chinensis* extract and 96.5% by weight of octyldodecanool (sample number Y5). The specific preparation steps of the composition are basically the same as those in Example 2, except that the caprylic / capric triglyceride in step 5 is replaced with octyldodecanool.

[0087] Example 6

[0088] This embodiment provides a composition comprising 1% by weight of *Saururus chinensis* extract and 99% by weight of octyldodecanool (sample number Y6). The specific preparation steps of the composition are the same as in Example 1.

[0089] Example 7

[0090] This embodiment provides a composition comprising 10% by weight of *Saururus chinensis* extract and 90% by weight of octyldodecanool (sample number Y7). The specific preparation steps of the composition are the same as in Example 1. In the composition prepared in this embodiment, the solubility of *Saururus chinensis* extract is greatly reduced, and the resulting composition has a darker color.

[0091] Example 8

[0092] This embodiment provides a composition comprising 5% by weight of *Saururus chinensis* extract and 95% by weight of octyldodecanool (sample number Y8). The specific preparation steps of the composition are the same as in Example 1.

[0093] Example 9

[0094] This embodiment provides a composition comprising 3.5% by weight of *Saururus chinensis* extract and 96.5% by weight of hexanediol (sample number Y9). The specific preparation steps of the composition are basically the same as those in Example 2, except that the caprylic / capric triglyceride in step 5 is replaced with hexanediol.

[0095] Example 10

[0096] This embodiment provides a composition comprising 3.5% by weight of *Saussurea involucrata* extract and 96.5% by weight of squalane (sample number Y10). The specific preparation steps of the composition are basically the same as those in Example 2, except that the caprylic / capric triglyceride in step 5 is replaced with squalane.

[0097] Example 11

[0098] This embodiment provides a composition comprising 3.5% by weight of *Saururus chinensis* extract and 96.5% by weight of sunflower seed oil (sample number Y11). The specific preparation steps of the composition are basically the same as those in Example 2, except that the caprylic / capric triglyceride in step 5 is replaced with sunflower seed oil.

[0099] Example 12

[0100] This embodiment provides a composition comprising 3.5% by weight of *Saussurea involucrata* extract and 96.5% by weight of polyglycerol-2 triisostearate (sample number Y12). The specific preparation steps of the composition are basically the same as those in Example 2, except that the caprylic / capric triglyceride in step 5 is replaced with polyglycerol-2 triisostearate.

[0101] Example 13

[0102] This embodiment provides a composition comprising 3% by weight of *Saururus chinensis* extract and 97% by weight of caprylic / capric triglyceride (sample number Y13). The specific preparation steps of the composition are as follows:

[0103] 1. Extraction: Cut the raw material of Saururus chinensis (stems and leaves) into sections, add 500.0g of raw material, add 6000mL of 60% ethanol each time, reflux at 80-90℃ for 1h, extract 3 times, combine the extracts, cool to room temperature, and filter the extract;

[0104] 2. Enrichment: The filtrate obtained in the extraction step was loaded into 1000 mL of LX-100B macroporous resin at a flow rate of 1.0 Bv / h; after loading, it was desorbed with 6000 mL of 80% ethanol at a flow rate of 3.0 Bv / h, and the desorbed solution was collected.

[0105] 3. Decolorization: Add 1.0g of 200-300 mesh coconut shell activated carbon to the eluent, stir at 60℃ for 20min for decolorization, and then filter using a 0.45um membrane; the resulting filtrate is then loaded onto LX-T5 anion exchange resin (the volume ratio of filtrate to LX-T5 anion exchange resin is 10:1) for decolorization, with a column flow rate of 1.0 Bv / h, and the eluent is collected and the pH is adjusted to 6.5-6.7 with citric acid; the color of the resulting decolorized solution is basically the same as that in Example 1.

[0106] 4. Concentration and Drying: The eluent obtained from the decolorization step was concentrated and dried under vacuum at a vacuum degree of 0.05 MPa, a drying temperature of 50°C, and a drying time of 20 hours. 3.2 g of extract (i.e., *Saururus chinensis* extract) was obtained.

[0107] 5. Dissolution with fat-soluble solvents: Take 2.0g of extract, add caprylic / capric triglyceride at a concentration of 3%, dissolve and filter to obtain a stable composition.

[0108] Example 14

[0109] This embodiment provides a composition comprising 4% by weight of *Saururus chinensis* extract and 96% by weight of caprylic / capric triglyceride (sample number Y14). The specific preparation steps of the composition are as follows:

[0110] 1. Extraction: Cut the raw material of Saururus chinensis (stems and leaves) into sections, add 500.0g of raw material, add 3000mL of 70% ethanol each time, reflux at 80-90℃ for 2h, extract twice, combine the extracts, cool to room temperature, and filter the extract;

[0111] 2. Enrichment: Load the filtrate obtained from the extraction step into 250 mL of LX-100B macroporous resin at a flow rate of 0.5 Bv / h; after loading, desorb with 250 mL of 90% ethanol at a flow rate of 0.5 Bv / h, and collect the desorbed solution.

[0112] 3. Decolorization: Add 5.0g of 200-300 mesh coconut shell activated carbon to the eluent, stir at 20℃ for 30min for decolorization, and then filter through a 0.45um membrane; the resulting filtrate is then loaded onto LX-T5 anion exchange resin (the volume ratio of filtrate to LX-T5 anion exchange resin is 30:1) for decolorization, with a column flow rate of 3.0 Bv / h, and the eluent is collected and the pH is adjusted to 6.7-6.9 with citric acid; the color of the resulting decolorized solution is basically the same as that in Example 1.

[0113] 4. Concentration and Drying: The eluent obtained from the decolorization step was concentrated and dried under vacuum at a vacuum degree of 0.05 MPa, a drying temperature of 70°C, and a drying time of 5 hours. 2.8 g of extract (i.e., *Saururus chinensis* extract) was obtained.

[0114] 5. Dissolution with fat-soluble solvents: Take 2.0g of extract, add caprylic / capric triglyceride at a concentration of 4%, dissolve and filter to obtain a stable composition.

[0115] Example 15

[0116] This embodiment provides a composition comprising 3.5% by weight of *Saururus chinensis* extract and 96.5% by weight of caprylic / capric triglyceride (sample number Y15). The specific preparation steps of the composition are as follows:

[0117] 1. Extraction: Cut the raw material of Saururus chinensis (stems and leaves) into sections, add 300.0g of raw material, add 3000mL of 50% ethanol each time, reflux at 80-90℃ for 2h, extract twice, combine the extracts, cool to room temperature, and filter the extract;

[0118] 2. Enrichment: The filtrate obtained from the extraction step was loaded onto 300 mL of D101 macroporous resin at a flow rate of 2.0 Bv / h. After loading, the solution was desorbed with 600 mL of 70% ethanol at a flow rate of 1.0 Bv / h, and the desorbed solution was collected.

[0119] 3. Concentration and Drying: The desorbed solution obtained in the enrichment step was concentrated and dried under vacuum at a vacuum degree of 0.05 MPa, a drying temperature of 70°C, and a drying time of 8 hours. 0.5 g of extract (i.e., *Saururus chinensis* extract) was obtained.

[0120] 4. Dissolution with a fat-soluble solvent: Take 0.4 g of the extract and add caprylic / capric triglyceride at a concentration of 3.5%. After dissolving and filtering, the composition is obtained. Since this comparative example was not decolorized, the prepared composition is darker in color than that of Example 1 and is less stable, with precipitation occurring after 1 day.

[0121] Comparative Example 1

[0122] This comparative example provides a composition comprising 0.3% by weight of triscutellarin and 99.7% by weight of caprylic / capric triglyceride (sample number Y16). The specific preparation steps of the composition are as follows:

[0123] The specific steps are as follows: Take 20 mg of triscutellarin standard, add caprylic / capric triglyceride at a concentration of 0.3%, dissolve and filter to obtain the composition.

[0124] Efficacy verification:

[0125] The compositions prepared in Examples 1-14 (sample numbers Y1-Y14) and Comparative Example 1 (sample number Y16) were formulated into test products with a concentration of 0.5% for either *Saururus chinensis* extract solution or *Saururus chinensis* ketone solution using matrix raw materials. The formulation ratios of each test product are shown in Table 1 below. The test products formulated from the extracts prepared in Examples 1-15 are designated as 0.5% Y1 to 0.5% Y14, and the test product formulated from the composition prepared in Comparative Example 1 is designated as 0.5% Y16.

[0126] Table 1

[0127] The preparation steps for each product to be tested are as follows:

[0128] 1. Heat phases A and B to 80-85℃ respectively, and stir until homogeneous.

[0129] 2. Add phase A to phase B and homogenize for 3 minutes.

[0130] 3. Continue stirring until the temperature reaches approximately 40°C, then add phase C.

[0131] In addition, control products with corresponding concentrations of 0.5% were prepared using the above-mentioned matrix raw materials, namely caprylic / capric triglyceride, macadamia nut oil, octyl dodecyl alcohol, ethylene glycol, squalane, sunflower seed oil, and polyglycerol-2 triisostearate.

[0132] Clinical redness-reducing tests were conducted on each test product and each solvent control product. The clinical redness-reducing test models included capsaicin stimulation, histamine stimulation, and methyl nicotinic acid stimulation. Capsaicin, histamine, and methyl nicotinic acid were purchased from the market, and the tests used in this study were approved by the Ethics Committee of Shanghai Jiakai Co., Ltd. (batch numbers: JKC-2409-0051, JKC-2409-0052). The concentrations of capsaicin, histamine, and methyl nicotinic acid used were 200 μg / mL, 20 mg / mL, and 20 mM, respectively.

[0133] The specific test plan is as follows:

[0134] ① After cleaning the inside of the arm, allow it to rest for 20 minutes, then use a marker to mark the test area (3*3cm) for each group. 2 Each test area should be spaced at least 1 cm apart;

[0135] ② Use a skin color meter (SkinColorCatch, brand: Delfin) to test the skin's baseline a* value (i.e., a* value). 刺激前 After applying 30 μL of irritant for 15 minutes, the a* value was measured using a probe. 刺激后 Take a photo with Vplus and record it as 0 minutes of repair.

[0136] ③Then apply 25 μL of each test sample or solvent control sample to the sample group; do not apply any sample to the irritant group.

[0137] After applying each test sample or solvent control sample for 30 minutes, the a* value (i.e., a* value) of the sample group and the stimulant group was measured again using the probe. 修护后 Take a photo with Vplus and record it as 30 minutes of repair.

[0138] The specific data analysis methods are as follows:

[0139] The Δa* value for each sample group and stimulus group was calculated using the following formula: Δa* value = a* value 修护后 -a* value 刺激前

[0140] The rate of change of Δa* value in the sample group compared to the stimulus group was calculated using the following formula:

[0141] Δa* value change rate (%) = (Δa* value) 样品组 / Δa* value 刺激物组 -1)*100%

[0142] Paired T-test, α=0.05, compared with the matrix group, *** represents P<0.001, ** represents P<0.01, * represents P<0.05, and ns represents P>0.05.

[0143] The results of the Δa* value change rate tests for each sample group after stimulation with various stimuli are shown in Table 2. The Δa* value change rate for each solvent control group was less than 5%.

[0144] Table 2

[0145] Figure 2 shows the alleviating effect of the test product 0.5% Y1 on skin redness induced by capsaicin, histamine, and methyl nicotinic acid. Compared with the irritant groups of capsaicin, histamine, and methyl nicotinic acid, the Δa* values ​​of 0.5% Y1 changed by -44.69%, -40.42%, and -30.04%, respectively.

[0146] The results of the alleviating effect of the test product 0.5% Y2 on skin redness induced by capsaicin, histamine, and methyl nicotinic acid are shown in Figures 3 and 4. Compared with the irritant groups of capsaicin, histamine, and methyl nicotinic acid, the Δa* values ​​of 0.5% Y2 changed by -57.41%, -72.59%, and -48.81%, respectively.

[0147] Figure 5 shows the alleviating effect of the test product 0.5% Y3 on skin redness induced by capsaicin, histamine, and methyl nicotinic acid. Compared with the irritant groups of capsaicin, histamine, and methyl nicotinic acid, the Δa* values ​​of 0.5% Y3 changed by -61.37%, -67.04%, and -48.21%, respectively.

[0148] The results of the alleviating effect of the test product 0.5% Y4 on skin redness induced by capsaicin, histamine, and methyl nicotinic acid are shown in Figure 6. Compared with the irritant groups of capsaicin, histamine, and methyl nicotinic acid, the Δa* value change rates of 0.5% Y4 were -61.90%, -65.81%, and -47.47%, respectively.

[0149] Figure 7 shows the alleviating effect of the test product 0.5% Y5 on skin redness induced by capsaicin, histamine, and methyl nicotinic acid. Compared with the irritant groups of capsaicin, histamine, and methyl nicotinic acid, the Δa* values ​​of 0.5% Y5 changed by -51.19%, -53.33%, and -43.65%, respectively.

[0150] The results of the alleviating effect of the test product 0.5% Y6 on skin redness induced by capsaicin, histamine, and methyl nicotinic acid are shown in Figure 8. Compared with the irritant groups of capsaicin, histamine, and methyl nicotinic acid, the Δa* value of 0.5% Y6 changed by -17.45%, -10.82%, and -10.91%, respectively.

[0151] Figure 9 shows the alleviating effect of the test product 0.5% Y7 on skin redness induced by capsaicin, histamine, and methyl nicotinic acid. Compared with the irritant groups of capsaicin, histamine, and methyl nicotinic acid, the Δa* values ​​of 0.5% Y7 changed by -40.92%, -22.77%, and -24.26%, respectively.

[0152] Figure 10 shows the alleviating effect of the test product 0.5% Y8 on skin redness induced by capsaicin, histamine, and methyl nicotinic acid. Compared with the irritant groups of capsaicin, histamine, and methyl nicotinic acid, the Δa* values ​​of 0.5% Y8 changed by -56.68%, -50.81%, and -49.74%, respectively.

[0153] Figure 11 shows the alleviating effect of the test product 0.5% Y9 on skin redness induced by capsaicin, histamine, and methyl nicotinic acid. Compared with the irritant groups of capsaicin, histamine, and methyl nicotinic acid, the Δa* values ​​of 0.5% Y9 changed by -46.74%, -42.89%, and -24.41%, respectively.

[0154] Figure 12 shows the alleviating effect of the test product 0.5% Y10 on skin redness induced by capsaicin, histamine, and methyl nicotinic acid. Compared with the irritant groups of capsaicin, histamine, and methyl nicotinic acid, the Δa* values ​​of 0.5% Y10 changed by -24.19%, -17.21%, and -6.25%, respectively.

[0155] Figure 13 shows the alleviating effect of the test product 0.5% Y11 on skin redness induced by capsaicin, histamine, and methyl nicotinic acid. Compared with the irritant groups of capsaicin, histamine, and methyl nicotinic acid, the Δa* values ​​of 0.5% Y11 changed by -65.72%, -54.17%, and -43.01%, respectively.

[0156] Figure 14 shows the alleviating effect of the test product 0.5% Y12 on skin redness induced by capsaicin, histamine, and methyl nicotinic acid. Compared with the irritant groups of capsaicin, histamine, and methyl nicotinic acid, the Δa* values ​​of 0.5% Y12 changed by -57.82%, -54.94%, and -49.87%, respectively.

[0157] Figure 15 shows the alleviating effect of the test product 0.5% Y13 on skin redness induced by capsaicin, histamine, and methyl nicotinic acid. Compared with the irritant groups of capsaicin, histamine, and methyl nicotinic acid, the Δa* values ​​of 0.5% Y13 changed by -55.13%, -44.41%, and -35.94%, respectively.

[0158] Figure 16 shows the alleviating effect of the test product 0.5% Y14 on skin redness induced by capsaicin, histamine, and methyl nicotinic acid. Compared with the irritant groups of capsaicin, histamine, and methyl nicotinic acid, the Δa* values ​​of 0.5% Y14 changed by -53.68%, -47.25%, and -42.19%, respectively.

[0159] Figure 17 shows the alleviating effect of the test product 0.5% Y16 on skin redness induced by capsaicin, histamine, and methyl nicotinic acid. Compared with the irritant groups of capsaicin, histamine, and methyl nicotinic acid, the Δa* values ​​of 0.5% Y16 changed by -26.89%, -14.08%, and -5.21%, respectively.

[0160] This invention has many specific applications, and the above description is only a preferred embodiment. It should be noted that the above embodiments are for illustrative purposes only and are not intended to limit the scope of protection of this invention. For those skilled in the art, several improvements can be made without departing from the principle of this invention, and these improvements should also be considered within the scope of protection of this invention.

Claims

1. A composition having redness-reducing effect, characterized in that, The components include the following components in the indicated mass percentages: 90-99% fat-soluble solvent and 1-10% *Saururus chinensis* extract; The fat-soluble solvent is selected from at least one of the following: acetodiol, caprylic / capric triglyceride, sunflower seed oil, macadamia nut oil, meadowfoam seed oil, silicone oil, squalane, ethylhexyl palmitate, diisostearyl malate, polyglycerol-2 triisostearyl, linseed oil, octyldodecanool, and cocoyl octanoate / capric ester.

2. The composition with redness-reducing effect according to claim 1, characterized in that, It includes the following components by weight percentage: 95-97% fat-soluble solvent and 3-5% *Saururus chinensis* extract.

3. The composition with redness-reducing effect according to claim 1, characterized in that, The fat-soluble solvent is selected from at least one of caprylic / capric triglyceride, macadamia nut oil, and octyldodecanool.

4. The composition with redness-reducing effect according to claim 1, characterized in that, The *Saururus chinensis* extract is a fat-soluble extract of *Saururus chinensis*.

5. The composition with redness-reducing effect according to claim 4, characterized in that, The extract of Saururus chinensis was prepared by the following method: The raw material of *Sanbaicao* is extracted with alcohol, then filtered, and then subjected to resin adsorption and alcohol desorption, followed by concentration and drying to obtain the final product.

6. The composition with redness-reducing effect according to claim 5, characterized in that, The alcohol is selected from at least one of ethanol, propanol, butanol, glycerol, and butanediol; The resin is a macroporous resin.

7. The composition with redness-reducing effect according to claim 5, characterized in that, The alcohol desorption step is followed by a decolorization step.

8. The composition with redness-reducing effect according to claim 5, 6, or 7, characterized in that, The preparation method of the *Trifolium repens* extract includes the following steps: A1. Extraction: After cutting the raw material of Sanbaicao into sections, it is extracted by reflux with ethanol at a volume fraction of 60-70%; A2. Enrichment: Filter the extract obtained in step A1, load the filtrate a onto a macroporous resin, and then desorb it using 80-95% ethanol by volume. Collect the desorbed liquid, concentrate and dry it to obtain the *Trifolium repens* extract.

9. The composition with redness-reducing effect according to claim 8, characterized in that, In step A2, the collected desorbed solution is further decolorized before being concentrated and dried. The decolorization process is as follows: the desorbed liquid is first decolorized with activated carbon and then filtered. The resulting filtrate b is then decolorized with anionic macroporous resin. The decolorized liquid is collected and the pH is adjusted to 6.5-7.

1.

10. The use of a composition according to any one of claims 1-9 in the preparation of a cosmetic having a redness-reducing effect, wherein the amount of the composition added is 0.01-10%.

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