Method for biotinylating target protein, method for protein coupling and use thereof
By combining the NHS removal reaction with the biotin-streptavidin system, efficient and low-damage protein conjugation was achieved, solving the problems of low conjugation efficiency and insufficient detection sensitivity in existing technologies, and improving the accuracy of detection signals and results.
Patent Information
- Authority / Receiving Office
- WO · WO
- Patent Type
- Applications
- Current Assignee / Owner
- MGI TECH CO LTD
- Filing Date
- 2024-11-20
- Publication Date
- 2026-05-28
AI Technical Summary
Existing protein conjugation methods result in chemical damage to the antibody and luciferase ends, leading to low conjugation efficiency and failing to meet the requirements for high detection signals and high detection sensitivity. In particular, in biotinylation labeling applications, biotinylation of antibodies or other target proteins may be accompanied by activity impairment or inactivation of the labeled protein due to over-labeling.
The target protein is specifically cross-linked with biotin compounds using an NHS removal reaction to generate a protein-biotin cross-linked complex. This complex then combines with the biotin-streptavidin system for multivalent binding and signal amplification, thereby improving detection sensitivity.
It improves protein coupling efficiency, reduces protein damage, enables cascade amplification of signals, and enhances detection sensitivity and accuracy.
Smart Images
Figure CN2024133182_28052026_PF_FP_ABST