Composition comprising rambutane extract, and preparation method therefor

Abstract

The present invention provides: a composition comprising a Nephelium lappaceum extract extracted with an extraction solvent comprising a natural deep eutectic solvent (NADES); and a preparation method therefor. Specifically, the present invention comprises a cosmetic composition for improving the condition of the skin and a pharmaceutical composition for preventing or treating inflammatory skin diseases, each comprising a Nephelium lappaceum extract extracted with an extraction solvent comprising a natural deep eutectic solvent.

Description

Composition containing rambutan extract and method for preparing the same

[0001] The present invention relates to a composition containing rambutan extract and a method for preparing the same, and more specifically, to a rambutan extract extracted using Natural Deep Eutectic Solvents (NADES), a composition for treating skin diseases and whitening the skin containing rambutan extract as an active ingredient, and a method for extracting an active ingredient from rambutan using Natural Deep Eutectic Solvents (NADES).

[0002] The skin serves as the body's primary defense barrier and is largely composed of the epidermis, dermis, and subcutaneous fat layer. It protects internal organs from external stimuli, such as changes in temperature and humidity, ultraviolet rays, and pollutants, and plays a crucial role in maintaining biological homeostasis, including thermoregulation. Because it is constantly exposed to external stimuli, the skin possesses diverse defensive capabilities. However, cellular damage stimuli applied from outside the cell—such as excessive physical and chemical irritations, stress, nutritional deficiencies, ultraviolet rays, environmental pollutants, and sudden temperature changes—induce various biochemical changes within the cell, triggering a cellular damage response. One such skin problem is skin darkening, including melasma, freckles, and blemishes, which is caused by the influence of melanin.

[0003] Melanin, present in the epidermal layer—the outermost layer of the skin—plays a useful role in protecting skin organs below the dermis by acting as a UV barrier. Simultaneously, it scavenges free radicals generated within the body, thereby safeguarding skin proteins and genes. When the skin is exposed to ultraviolet (UV) rays, an enzyme called tyrosinase acts on tyrosine, an amino acid, converting it into DOPA and dopaquinone. Subsequently, through non-enzymatic oxidation reactions, a polymer called melanin is synthesized within melanosomes inside melanocytes, which are skin pigment cells. Furthermore, melanin production increases when the generation of free radicals in the skin rises, inflammatory reactions occur, or exposure to UV rays is present.

[0004] As the causes and mechanisms of such skin darkening have been elucidated, substances that inhibit tyrosinase, an enzyme involved in the skin darkening process, or suppress melanin polymerization reactions are being incorporated into cosmetics. Representative substances used for this purpose include hydroquinone, ascorbic acid, kojic acid, thiol compounds such as glutathione and cysteine, and alpha hydroxy acids.

[0005] However, most of these ingredients are known to be inherently unstable, have weak whitening effects, and cause skin irritation. Additionally, natural extracts known for their whitening effects contain only trace amounts of active ingredients, resulting in minimal whitening. Furthermore, the content varies significantly depending on the region or harvest time, making it difficult to maintain quality homogeneity and raising safety concerns for the skin. Additionally, there are formulation stability issues when used in cosmetic formulations, and the co-extraction of various compounds leads to disadvantages such as skin side effects and discoloration.

[0006] Furthermore, as skin serves as a marker of beauty, healthy and clear skin is directly linked to a person's attractiveness; however, as people age, skin aging occurs, with wrinkles being a representative symptom. Wrinkles are a phenomenon indicating age, and the primary cause of their formation is the breakdown of collagen, which forms the matrix in the dermis. While the production of collagen in the skin may decrease as aging progresses, there are also numerous clinical reports indicating that external stimuli, such as ultraviolet rays, increase the activity of the collagen-degrading enzyme (Matrix Metalloproteinase, MMP-1), leading to the easy breakdown of collagen and an increase in wrinkle formation. Consequently, many products have recently been developed to fundamentally inhibit the breakdown of collagen by suppressing the production and activity of MMP-1.

[0007] Meanwhile, MMPs are calcium and zinc-dependent endopeptidases secreted by cells such as polymorphonuclear neutrophils, macrophages, and fibroblasts; they operate at a neutral pH and utilize the substrates of various cells. In the skin, various enzymes other than MMP-1, MMP-2, and MMP-9 are active, but the enzyme that acts most fundamentally in relation to collagen degradation is MMP-1. Conventional substances that generally promote collagen synthesis include retinoids (RE36068), TGF-β (Transforming growth factor), and betulinic acid (JP8-208424). TGF-β (Transforming growth factor) is a representative substance known to inhibit the production of MMP-1, and various other natural substances are also known to inhibit MMP-1 biosynthesis.

[0008] Organic solvents have long been commonly used for liquid extraction. While organic solvents are inexpensive and offer excellent extraction efficiency, they have drawbacks, such as being harmful to the health of researchers and potentially causing environmental pollution. Recently, research on various "green solvents" has been conducted to overcome these disadvantages. Consequently, the use of ionic liquids or deep eutectic solvents has been proposed as alternatives to organic solvents, leading to active research in this area.

[0009] Ionic liquids are salts formed by the combination of macrocyclic cations and various inorganic anions, and they exist as liquids at room temperature due to their low melting point. Ionic liquids have been applied in extraction and separation fields because they possess low boiling points, low vapor pressure, non-volatility, low melting point, and high ionic conductivity. However, on the other hand, these ionic liquids have disadvantages such as toxicity harmful to the human body, low biodegradability, and the fact that their physical properties can be affected by trace impurities. Furthermore, because a large amount of salt and solvent are used for the complete exchange of anions during the synthesis of ionic liquids, they are unsuitable for environmentally friendly solvents and are expensive.

[0010] Therefore, to overcome these drawbacks, research on new clean solvents such as deep eutectic solvents (DES) is necessary, and there is a need to develop natural extracts using these solvents that are effective in improving skin condition and harmless to the human body.

[0011] The present invention provides a rambutan extract extracted with an extraction solvent comprising natural deep eutectic solvents (NADES).

[0012] In addition, the present invention provides a cosmetic composition for improving skin condition comprising the above-mentioned rambutan extract.

[0013] In addition, the present invention provides a skin external preparation composition for improving skin condition comprising the above-mentioned rambutan extract.

[0014] In addition, the present invention provides a pharmaceutical composition for the prevention or treatment of inflammatory skin diseases comprising the rambutan extract.

[0015] In addition, the present invention provides a food composition for improving skin condition comprising the above-mentioned rambutan extract.

[0016] In addition, the present invention provides a food composition for the prevention or improvement of inflammatory skin diseases comprising the above-mentioned rambutan extract.

[0017] In addition, the present invention provides a health functional food for improving skin condition comprising the above-mentioned rambutan extract.

[0018] In addition, the present invention provides a health functional food for the prevention or improvement of inflammatory skin diseases comprising the above-mentioned rambutan extract.

[0019] In addition, the present invention provides a method for preventing, improving, or treating the condition of an individual, comprising the step of treating or administering to the individual a composition comprising a rambutan extract extracted with an extraction solvent including natural deep eutectic solvents (NADES).

[0020] In another aspect of the present invention, the present invention provides a use of the rambutan extract according to the present invention for the manufacture of a drug for the treatment of inflammatory skin diseases.

[0021] In addition, in another aspect of the present invention, the present invention provides a rambutan extract for use in treating inflammatory skin diseases or improving skin conditions.

[0022] In addition, the present invention provides a method for preparing a rambutan extract comprising the step of extracting rambutan with an extraction solvent including a natural eutectic solvent (NADES).

[0023] The present invention is capable of various modifications and may have various embodiments, and specific embodiments are to be illustrated and described in detail. However, this is not intended to limit the invention to specific embodiments, and it should be understood that the invention includes all modifications, equivalents, and substitutions that fall within the spirit and scope of the invention.

[0024] The terms used in this application are used merely to describe specific embodiments and are not intended to limit the invention. The singular expression includes the plural expression unless the context clearly indicates otherwise. In this application, terms such as "comprising" or "having" are intended to indicate the presence of the features, numbers, steps, actions, components, parts, or combinations thereof described in the specification, and should be understood as not precluding the existence or addition of one or more other features, numbers, steps, actions, components, parts, or combinations thereof.

[0025] Where in this specification, when a quantity, concentration, or other value or parameter is given as an enumeration of a range, a preferred range, a preferred upper limit, and a preferred lower limit, it should be understood that any pair of any upper range limit or preferred value and any lower range limit or preferred value are specifically disclosed, regardless of whether the range is disclosed separately.

[0026] Where a range of numerical values ​​is mentioned in this specification, unless otherwise stated, the range and the scope of the present invention within that range are not intended to be limited to the specific values ​​mentioned when defining the range. Unless otherwise defined, all terms used herein, including technical or scientific terms, have the same meaning as generally understood by those skilled in the art to which the present invention pertains. Terms such as those defined in commonly used dictionaries should be interpreted as having a meaning consistent with their meaning in the context of the relevant technology, and should not be interpreted in an ideal or overly formal sense unless explicitly defined in this application. Specific details for the implementation of the above invention are described below.

[0027] Composition comprising rambutan extract extracted with a natural eutectic solvent

[0028] According to one aspect of the present invention, a cosmetic composition for improving skin condition is provided, comprising a rambutan extract extracted with an extraction solvent including natural deep eutectic solvents (NADES).

[0029] In this invention, the term "rambutan (Nepheliumlappaceum)" refers to a fruit belonging to the genus Nephelium that is widely distributed in Southeast Asia. The tree reaches a height of 10 to 15 meters, and its leaves are alternate and pinnately compound, consisting of 5 to 7 pairs; they are thick and glossy, with hairs on the underside when young. Flowers bloom from April to June, and the fruits grow in clusters of 10 to 12. They are about the size of a small egg, ripen to a red color in July and August, and have fleshy protrusions. Rambutan is one of the most well-known tropical fruits in Southeast Asia, and Thailand is the largest producer. Cultivated fruit is usually consumed fresh after removing the peel and seeds; it is also used in cooking in jam or cooked form, as well as in canned form for syrup. It is a commercially important crop in Asia, and in Malaysia, dried fruit peels are used in local medicine, while the fruit is consumed worldwide.

[0030] The rambutan extract of the present invention can be extracted from leaves, fruits, fruit peels, roots, branches, stem parts, or plant tissue cultures of plants that are directly cultivated or commercially available. Preferably, the rambutan extract of the present invention may be an extract obtained from the peel of a rambutan fruit. In addition, the extract may be extracted from natural, hybrid, or variant plants without limitation.

[0031] In the present invention, the term "extract" refers to a product such as a liquid component obtained by immersing a target substance in various solvents and then extracting it for a certain period of time at room temperature, low temperature, or heated conditions, or a solid component obtained by removing the solvent from said liquid component. Furthermore, it may be comprehensively interpreted to include all of the above-mentioned products, such as diluted solutions, concentrates thereof, dried products obtained by drying the extracts, or modified or purified products thereof.

[0032] In the present invention, the "rambutan extract" may be a concentrate obtained by concentrating a filtered liquid of rambutan extract, and the "rambutan purified product" may be obtained by purifying a re-filtered liquid obtained by filtering the concentrate.

[0033] The above "Natural Deep Eutectic Solvent" refers to a substance that exists in a solution state at 40–50°C or below, with a lowered melting point when two naturally derived substances (liquid / solid or solid / solid) are mixed in a specific molar ratio at room temperature. The above natural deep eutectic solvent is known as a natural solvent recently used instead of alcohol or acetone in natural product extraction processes. The above natural deep eutectic solvent includes hydrophilic solvents that dissolve in water and hydrophobic solvents that dissolve in oil.

[0034] In the present invention, the natural eutectic solvent is in a state where a salt (or hydrogen bond acceptor) and a hydrogen bond donor (HBD, hydrogen bond donors) are eutecticed by hydrogen bonding, and can be prepared by mixing the salt and HBD in a certain ratio. The extract according to the present invention can be environmentally friendly and economical because it extracts useful physiologically active substances from rambutan using a natural eutectic solvent.

[0035] The above salt may be one or more selected from the group consisting of betaine, L-proline, and sucrose. Meanwhile, as a hydrogen bond donor, it may include one or more hydrogen bond donors selected from the group consisting of amines, alcohols, and sugars, and may include, for example, propanediol, glycerin, xylitol, and sorbitol.

[0036] Preferably, the salt may include betaine, and the hydrogen bond donor may include propanediol.

[0037] In this invention, to maximize the extraction of active ingredients, betaine was used as the salt and propanediol was used as the hydrogen bond donor to optimize the composition and content ratio of the eutectic solvent.

[0038] Specifically, the above natural eutectic solvent (NADES) may have a molar ratio of betaine to propanediol of 1:1 to 1:4, preferably 1:1 to 1:3, more preferably 1:1.5 to 1:2.5, and most preferably 1:2. When prepared by mixing the above betaine to propanediol in a molar ratio of 1:1 to 1:4, the extraction efficiency is high, and geranine and corillazin, which are active components of the extract, are extracted in high quantities, which is desirable.

[0039] Although a natural eutectic solvent can be used directly as an extraction solvent, it may be mixed with other solvents to increase extraction efficiency due to its high viscosity. Accordingly, the extraction solvent may further contain water, and the extraction solvent may be used as a solvent mixed with the natural eutectic solvent and water.

[0040] In the present invention, the extraction solvent may be a mixed solvent comprising betaine, propanediol, and water.

[0041] Specifically, with respect to 100 parts by weight of the extraction solvent, it may comprise 40 to 90 parts by weight of the natural eutectic solvent (NADES) and 10 to 60 parts by weight of water, preferably 45 to 90 parts by weight of the natural eutectic solvent (NADES) and 10 to 55 parts by weight of water, more preferably 50 to 90 parts by weight of the natural eutectic solvent (NADES) and 10 to 50 parts by weight of water.

[0042] In addition, in the present invention, the extraction solvent may be used as a natural eutectic solvent alone, and preferably, the extraction solvent may be a natural eutectic solvent comprising betaine and propanediol.

[0043] Specifically, for every 100 parts by weight of the extraction solvent, 100 parts by weight of a natural eutectic solvent (NADES) may be included.

[0044] Specifically, the composition may contain 0.01 to 20 weight% of the rambutan extract based on the total weight of the composition, for example, the lower limit may be 0.05 weight% or more, 0.08 weight% or more, 0.1 weight% or more, 0.15 weight% or more, 0.18 weight% or more, 0.2 weight% or more, 0.25 weight% or more, 0.3 weight% or more, 0.35 weight% or more, 0.4 weight% or more, 0.45 weight% or more, or 0.5 weight% or more, and the upper limit may be 18 weight% or less, 16 weight% or less, 14 weight% or less, 12 weight% or less, 10 weight% or less, 8 weight% or less, 6 weight% or less, 4 weight% or less, 2 weight% or less, 1.5 weight% or less, or 1.2 weight% or less. If the above rambutan extract is included in an amount of less than 0.01% by weight, the skin disease improvement and whitening effects are negligible and therefore undesirable.

[0045] In addition, the composition containing the above rambutan extract can have a skin whitening effect by inhibiting melanin synthesis.

[0046] The above-mentioned improvement of skin condition may include, but is not limited to, skin moisturization, relief of redness, relief of itching, anti-inflammation, relief of skin irritation, improvement of skin barrier, skin soothing, skin whitening, and improvement of skin aging.

[0047] In the present invention, the term "skin barrier improvement" may refer to any action that alleviates skin diseases resulting from weakened skin barrier function, such as psoriasis, contact dermatitis, eczematous dermatitis, photodermatitis, seborrheic dermatitis, herpetic dermatitis, lichen planus, lichen sclerosus, gangrenous pyoderma, pemphigus, bullous epidermolysis, systemic sclerosis, or leprosy, or improves damaged skin barrier function.

[0048] The above cosmetic composition may be prepared in any formulation conventionally manufactured in the technical field to which the present invention belongs. For example, it may be formulated into softening lotions, nourishing lotions, emulsions, lotions, creams, pastes, gels, solutions, suspensions, oils, waxes, packs, powders, foundations, sprays, surfactant-containing cleansers, etc., but is not limited thereto. More specifically, it may be prepared in the form of skin lotion, skin softener, skin toner, nourishing cream, massage cream, milk lotion, powder, essence, eye cream, sun lotion, sun cream, makeup primer, makeup base, BB cream, powder foundation, emulsion foundation, cleansing cream, cleansing foam, cleansing water, soap, pack, stick-type product, balm-type product, spray, or powder formulation.

[0049] The above cosmetic composition may be any one of the formulations of a lotion, serum, essence, lotion, cream, powder, foundation, pack, patch, mask sheet, gel ointment, spray, and cleanser.

[0050] The above cosmetic composition may further include any conventional cosmetic ingredient selected from additional ingredients commonly used in cosmetics, such as thickeners, dispersants, fragrances, fillers, preservatives, antiseptics, neutralizing agents, sweeteners, vitamins, free radical scavengers, metal ion chelating agents, functional ingredients, and mixtures thereof. A person skilled in the art may select any additional ingredient and / or the amount thereof so that the advantageous properties of the composition according to this specification are not adversely affected or are substantially affected by the expected addition.

[0051] In the case where the above cosmetic composition is a surfactant-containing cleansing formulation, it may further include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linolin derivative, or ethoxylated glycerol fatty acid ester, etc., as a carrier component.

[0052] In the case where the cosmetic composition according to the present invention is in the form of a cream or gel, it may further include animal oil, vegetable oil, wax, paraffin, starch, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide as a carrier component.

[0053] In the case where the above cosmetic composition is in the form of a solution or emulsion, it may further include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, propylene glycol, glycerol aliphatic ester, polyethylene glycol, or fatty acid ester of sorbitan as a solvent, solvating agent, or emulsifying agent.

[0054] In the case where the above cosmetic composition is in the form of a suspension, it may further include, as a carrier component, a liquid diluent such as water, ethanol, or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracanth.

[0055] In the case where the above cosmetic composition is in the form of a powder or spray, it may further include lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder as a carrier component, and in particular, in the case of a spray formulation, it may further include a propellant such as chlorofluorohydrocarbon, propane / butane, or dimethyl ether.

[0056] In the present invention, the cosmetic composition may include rambutan extract, C10-30 alkyl acrylate crosspolymer which is an acrylate, ammonium acryloyl dimethyl taurate which is a VP copolymer, glycerin, butylene glycol, disodium EDTA, sodium gluconate, ethylhexylglycerin, polyglyceryl-10 laurate, hydroxyacetophenone, dipropylene glycol, caprylyl glycol, dipotassium glycyrrhizate, butylene glycol, purified water, sorbitan stearate, cetearyl oliveate, sorbitan oliveate, coconut oil, carrot extract (extracted by MCT), dimethicone, isopropyl myristate, caprylic triglyceride, vinyl dimethicone crosspolymer which is a dimethicone, dimethicone or arginine, etc.

[0057] The above cosmetic composition may be used by applying it alone or in combination, or by applying it in combination with other cosmetic compositions other than the present invention. In addition, the cosmetic composition according to the present invention may be used according to conventional methods of use, and the frequency of use may be varied depending on the user's skin condition or preference.

[0058] In addition, for each formulation of a cosmetic composition, appropriate ingredients may be selected and combined according to the formulation or intended use of the cosmetic. Since the ingredients and methods of combination may follow conventional technology, a specific description thereof is omitted in this specification.

[0059] According to another aspect of the present invention, a topical skin composition for improving skin condition is provided, comprising a rambutan extract extracted with an extraction solvent including natural deep eutectic solvents (NADES).

[0060] The above-mentioned topical preparation may be a cream, gel, ointment, skin emulsifier, skin suspension, transdermal delivery patch, drug-containing bandage, lotion, or a combination thereof. The above-mentioned topical preparation may be appropriately formulated as needed with ingredients commonly used in topical preparations for the skin, such as cosmetics or pharmaceuticals, for example, aqueous ingredients, oily ingredients, powder ingredients, alcohols, moisturizers, thickeners, UV absorbers, whitening agents, preservatives, antioxidants, surfactants, fragrances, colorants, various skin nutrients, or combinations thereof. The above topical skin preparation may also appropriately contain metal chelating agents such as disodium edetate, trisodium edetate, sodium citrate, sodium polyphosphate, sodium metaphosphate, and gluconic acid; caffeine, tannin, bellapamil, glablidin, various herbal medicines, tocopherol acetate, glycyrrhizic acid, tranexamic acid and its derivatives or salts thereof; and sugars such as vitamin C, magnesium ascorbate phosphate, ascorbate glucoside, arbutin, kojic acid, glucose, fructose, and trehalose.

[0061] According to another aspect of the present invention, a pharmaceutical composition for the prevention or treatment of inflammatory skin diseases is provided, comprising a rambutan (Nephelium lappaceum) extract extracted with an extraction solvent comprising natural deep eutectic solvents (NADES).

[0062] The descriptions of the above "natural eutectic solvent," "extraction solvent," and "rambutan extract" are as previously stated.

[0063] The above "inflammatory skin disease" may be a general term for skin diseases in which inflammation is the main lesion, and may be one or more selected from the group consisting of pruritus, atopic dermatitis, chronic recurrent dermatitis, contact dermatitis, seborrheic dermatitis, neurodermatitis, xerosis, erythema, inflammatory dermatitis, psoriasis, urticaria, pruritus, dry eczema, lupus erythematosus, and atopic diseases, but is not limited thereto.

[0064] The pharmaceutical composition of the present invention may include a pharmaceutically acceptable carrier and may be formulated according to conventional methods into oral formulations such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, and aerosols, as well as topical preparations, suppositories, and sterile injectable solutions.

[0065] The above-mentioned pharmaceutically acceptable carriers include, but are not limited to, those commonly used in the art, such as lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia gum, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, and mineral oil. Additionally, the pharmaceutical composition of the present invention comprises fillers, extenders, binders, wetting agents, disintegrants, diluents or excipients such as surfactants, and other pharmaceutically acceptable additives.

[0066] When the pharmaceutical composition of the present invention is formulated into an oral solid dosage form, it includes tablets, pills, powders, granules, capsules, etc., and such solid dosage forms may include at least one excipient, such as starch, calcium carbonate, sucrose or lactose, gelatin, etc., and may include, but are not limited to, lubricants such as magnesium stearate, talc, etc.

[0067] When the pharmaceutical composition of the present invention is formulated into an oral liquid form, it includes suspensions, liquid formulations, emulsions, syrups, etc., and includes diluents such as water and liquid paraffin, humectants, sweeteners, flavorings, preservatives, etc., but is not limited thereto.

[0068] When the pharmaceutical composition of the present invention is formulated for parenteral administration, it comprises a sterile aqueous solution, a non-aqueous solvent, a suspension agent, an emulsion, a lyophilized formulation, and a suppository. The non-aqueous solvent and suspension agent may include, but are not limited to, propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate. The base of the suppository may include, but is not limited to, Witepsol, Macrogol, Tween 61, cocoa gluten, laurin gluten, glycerogelatin, etc.

[0069] The dosage of the rambutan extract contained in the pharmaceutical composition of the present invention may vary depending on the patient's condition, body weight, age, degree of disease, drug form, route of administration, and duration, but can be appropriately selected by a person skilled in the art. For example, the extract may be administered at a dose of 1 to 2000 mg / kg per day, preferably 10 to 2000 mg / kg, and the administration may be given once a day or divided into several doses.

[0070] The pharmaceutical composition of the present invention may be administered to mammals such as rats, mice, livestock, and humans by various routes, for example, orally, intraperitoneally or intravenously, intramuscularly, subcutaneously, intrauterine dura mater, or intracerebroventricular injection.

[0071] The pharmaceutical composition according to the present invention has an anti-inflammatory effect and is effective in preventing or treating inflammatory skin diseases.

[0072] According to another aspect of the present invention, a food composition and a health functional food are provided that include a rambutan (Nephelium lappaceum) extract extracted with an extraction solvent comprising natural deep eutectic solvents (NADES).

[0073] The present invention provides a food composition for improving skin condition, preventing or improving inflammatory skin diseases, comprising a rambutan (Nephelium lappaceum) extract extracted with an extraction solvent including natural deep eutectic solvents (NADES).

[0074] The descriptions of the above "natural eutectic solvent," "extraction solvent," "rambutan extract," and "skin condition improvement" are as previously stated.

[0075] The present invention provides a health functional food for improving skin condition, preventing or improving inflammatory skin diseases, comprising a rambutan (Nephelium lappaceum) extract extracted with an extraction solvent including natural deep eutectic solvents (NADES).

[0076] The descriptions of the above "natural eutectic solvent," "extraction solvent," "rambutan extract," and "inflammatory skin disease" are as previously stated.

[0077] The food composition of the present invention may include conventional food additives, and unless otherwise specified, suitability as a "food additive" is determined in accordance with the specifications and standards for the relevant item, in accordance with the general provisions and general test methods of the food additive code approved by the Ministry of Food and Drug Safety.

[0078] Examples of items listed in the above "Food Additives Codex" include chemically synthesized products such as ketones, glycine, potassium citrate, nicotinic acid, and cinnamon acid; natural additives such as persimmon dye, licorice extract, crystalline cellulose, sorghum dye, and guar gum; and mixed preparations such as L-sodium glutamate preparations, alkaline noodle additives, preservative preparations, and tar dye preparations.

[0079] The food composition of the present invention may contain 0.01 to 20 weight percent of the rambutan extract of the present invention based on the total weight of the composition for the purpose of improving skin condition and preventing and / or improving inflammatory skin diseases.

[0080] The rambutan extract according to the present invention contained in the food composition of the present invention can be obtained by the same method as the extraction method mentioned in the preparation of the pharmaceutical composition.

[0081] In addition, the food composition of the present invention can be manufactured and processed in the form of tablets, capsules, powders, granules, liquids, pills, etc. for the purpose of improving skin condition and preventing and / or improving inflammatory skin diseases.

[0082] The food composition of the present invention may be used as a health functional food. The term "health functional food" refers to a food manufactured and processed using raw materials or ingredients having functional properties useful to the human body in accordance with the Health Functional Foods Act, and the term "functionality" refers to consuming the food for the purpose of obtaining useful effects for health purposes, such as regulating nutrients or physiological actions on the structure and function of the human body.

[0083] The health functional food of the present invention can be manufactured by methods commonly used in the industry, and can be manufactured by adding raw materials and ingredients commonly added in the industry. Furthermore, the formulation of the health functional food can be manufactured without restriction as long as it is a formulation recognized as a health functional food. The food composition of the present invention can be manufactured in various forms of formulations. Unlike general pharmaceuticals, it has the advantage of being made from food ingredients and thus free from side effects that may occur with long-term use of pharmaceuticals. Additionally, due to its excellent portability, the health functional food of the present invention can be consumed as an adjuvant to enhance the effects of improving skin condition and preventing and / or improving inflammatory skin diseases.

[0084] A method for preventing, improving, or treating the condition of an individual comprising the step of treating or administering a composition containing a rambutan extract of the present invention to an individual in need thereof, and a use thereof

[0085] In another aspect of the present invention, a method for preventing, improving, or treating a condition of an individual is provided, comprising the step of treating or administering to the individual a composition comprising a rambutan (Nephelium lappaceum) extract extracted with an extraction solvent comprising natural deep eutectic solvents (NADES).

[0086] In another aspect of the invention, a method for preventing, improving, or treating a condition of an individual is provided, comprising the step of treating or administering to the individual a rambutan (Nephelium lappaceum) extract extracted with an extraction solvent comprising natural deep eutectic solvents (NADES).

[0087] The descriptions of the above "natural eutectic solvent," "extraction solvent," and "rambutan extract" are as previously stated.

[0088] The condition of the above-mentioned entity may include conditions related to the skin, for example, skin moisturization, relief of redness, itching, inflammatory skin diseases, skin irritation, improvement of the skin barrier, skin soothing, skin whitening, and skin aging.

[0089] Accordingly, the method according to the present invention provides a method for improving or treating conditions related to skin moisturization, redness relief, itching, inflammatory skin diseases, skin irritation, skin barrier improvement, skin soothing, skin whitening, and skin aging in an individual, comprising, for example, the step of treating or administering to an individual an extract of rambutan (Nephelium lappaceum) extracted with natural deep eutectic solvents (NADES).

[0090] The above composition may be applied topically to an area requiring improvement of skin condition or improvement or treatment of inflammatory skin diseases, or administered by intradermal injection.

[0091] Administration may be performed by methods known in the art. Administration may be performed directly to the individual by any means, via routes such as, for example, intravenous, intramuscular, oral, transdermal, mucosal, intranasal, intratracheal, or subcutaneous administration. The said administration may be systemic or local. The dosage may vary depending on factors such as the formulation method, mode of administration, the patient's age, body weight, sex, pathological condition, diet, time of administration, route of administration, elimination rate, and response sensitivity, and a person skilled in the art can appropriately adjust the dosage by taking these factors into account.

[0092] The number of administrations may be once a day or two or more times within the range of clinically acceptable side effects, and the administration may be performed at one or two or more sites, and the total duration of administration may range from 1 to 30 days per treatment, administered daily or at intervals of 2 to 5 days. If necessary, the same treatment may be repeated after the appropriate time. For animals other than humans, the dosage may be the same as that for humans per kg, or an amount calculated by converting the above dosage based on, for example, the ratio of organ (e.g., heart) volumes between the target animal and humans (e.g., average value).

[0093] In another aspect of the present invention, the present invention provides a use of the rambutan extract according to the present invention for the manufacture of a drug for the treatment of inflammatory skin diseases.

[0094] In another aspect of the present invention, the present invention provides a use of rambutan extract in the manufacture of a drug for improving or treating conditions related to skin moisturization, redness relief, itching, inflammatory skin diseases, skin irritation, skin barrier improvement, skin soothing, skin whitening, and skin aging.

[0095] In addition, in another aspect of the present invention, the present invention provides a rambutan extract for use in treating inflammatory skin diseases or improving skin conditions.

[0096] In addition, in another aspect of the present invention, the present invention provides a rambutan extract for improving or treating conditions related to skin moisturization, redness relief, itching, inflammatory skin diseases, skin irritation, skin barrier improvement, skin soothing, skin whitening, and skin aging.

[0097] Method for preparing rambutan extract of the present invention

[0098] According to another aspect of the present invention, a method for preparing a rambutan extract is provided, comprising the step of extracting rambutan (Nephelium lappaceum) with an extraction solvent comprising natural deep eutectic solvents (NADES).

[0099] Specifically, the above natural eutectic solvent (NADES) may have a molar ratio of betaine to propanediol of 1:1 to 1:4, preferably 1:1 to 1:3, more preferably 1:1.5 to 1:2.5, and most preferably 1:2. When prepared by mixing the above betaine to propanediol in a molar ratio of 1:1 to 1:4, the extraction efficiency is high, and geranine and corillazin, which are active components of the extract, are extracted in high quantities, which is desirable.

[0100] Although a natural eutectic solvent can be used directly as an extraction solvent, it may be mixed with other solvents to increase extraction efficiency due to its high viscosity. Accordingly, the extraction solvent may further contain water, and the extraction solvent may be used as a solvent mixed with the natural eutectic solvent and water.

[0101] In the present invention, the extraction solvent may be a mixed solvent comprising betaine, propanediol, and water.

[0102] Specifically, with respect to 100 parts by weight of the extraction solvent, it may comprise 40 to 90 parts by weight of the natural eutectic solvent (NADES) and 10 to 60 parts by weight of water, preferably 45 to 90 parts by weight of the natural eutectic solvent (NADES) and 10 to 55 parts by weight of water, more preferably 50 to 90 parts by weight of the natural eutectic solvent (NADES) and 10 to 50 parts by weight of water.

[0103] In addition, in the present invention, the extraction solvent may be used as a natural eutectic solvent alone, and preferably, the extraction solvent may be a natural eutectic solvent comprising betaine and propanediol.

[0104] Specifically, for every 100 parts by weight of the extraction solvent, 100 parts by weight of a natural eutectic solvent (NADES) may be included.

[0105] In addition, the above extraction may be performed by shaking extraction, stirring extraction, or ultrasonic treatment.

[0106] In addition, the method for preparing the rambutan extract may further include the step of concentrating and filtering the extracted extract.

[0107] In the present invention, the step of extracting the rambutan extract can be performed in the following order.

[0108] (a) Step of preparing rambutan powder

[0109] (b) a step of mixing the above rambutan powder and an extraction solvent including a natural eutectic solvent (NADES), stirring to perform stirring extraction, and

[0110] (c) A step of obtaining rambutan extract by filtering after the above stirring extraction.

[0111] In step (a) above, the rambutan powder may be obtained by pulverizing the peel of the rambutan from which the flesh has been removed.

[0112] In step (b) above, the stirring extraction can be performed at a temperature of 50 to 100°C, preferably 60 to 90°C, and more preferably around 80°C.

[0113] Meanwhile, it should be understood that parts of the extraction method of the present invention, such as the extraction time and the number of extractions, which can be easily modified and applied by a person skilled in the art, are included in the present invention. However, regarding the extraction time, for example, it may be 5 minutes to 24 hours, 15 minutes to 12 hours, or 30 minutes to 2 hours, and most preferably around 1 hour, and the extraction time may be changed depending on the volume of the object to be extracted. In addition, the number of extractions may be 1 to 5 times, and preferably 1 to 4 times, but is not limited thereto.

[0114] In step (c) above, the extract obtained in step (b) is centrifuged to obtain the supernatant, and the obtained supernatant is filtered to remove impurities, thereby obtaining a rambutan extract.

[0115] Meanwhile, the above filtration may use conventional filtration separation methods, for example, conventional methods used to separate or filter solid components and liquid components, and may separate the filtrate and residue using filter paper, for example. In addition, the filtration here may be a filtration step that is repeated in a first, second, third, or more stages, and a method that allows as much active ingredient as possible to be extracted may be used.

[0116] In another aspect of the present invention, the rambutan extract may additionally include a separated or purified fraction or fraction material.

[0117] As used in the present invention, the term "fraction" refers to a result obtained by performing fractionation to separate a specific component or a specific group of components from a mixture containing various constituent components.

[0118] Meanwhile, the above fraction or fraction material may refer to a more isolated / purified form. For example, a fraction obtained by passing a rambutan extract through an ultrafiltration membrane having a certain molecular weight cut-off value, or a fraction obtained through various additional purification methods such as separation by various chromatography (designed for separation based on size, charge, hydrophobicity, or affinity), is also included in the rambutan fraction or fraction material.

[0119] By including the active ingredient of rambutan extract extracted using an eco-friendly and harmless extraction solvent according to the present invention in the composition, there is excellent improvement of skin condition, prevention or treatment of skin diseases, and skin whitening effect.

[0120] Figure 1 is a graph showing the change in expression of the inflammatory cytokine TNF-α after treating LPS-stimulated cells with rambutan extracts according to Example 1 (NADES solvent) and Comparative Example 1 (50% ethanol aqueous solvent).

[0121] Figure 2 is a graph showing the change in the expression of the inflammatory cytokine IL-1α after treating LPS-stimulated cells with rambutan extracts according to Example 1 (NADES solvent) and Comparative Example 1 (50% ethanol aqueous solvent).

[0122] Figure 3 is a graph showing the change in expression of the inflammatory cytokine TNF-α after treating UV-stimulated cells with rambutan extracts according to Example 1 (NADES solvent) and Comparative Example 1 (50% ethanol aqueous solvent).

[0123] Figure 4 is a graph showing the change in the expression of the inflammatory cytokine IL-1α after treating UV-stimulated cells with rambutan extracts according to Example 1 (NADES solvent) and Comparative Example 1 (50% ethanol aqueous solvent).

[0124] Figure 5 is a graph showing the change in melanin concentration after treating cells stimulated by α-MSH with rambutan extracts according to Example 1 (NADES solvent) and Comparative Example 1 (50% ethanol aqueous solvent).

[0125] Figure 6 is a graph showing the change in MMP-1 protein expression after treating cells with rambutan extracts according to Example 1 (NADES solvent) and Comparative Example 1 (50% ethanol aqueous solvent).

[0126] Figure 7 is a graph showing the change in MMP-9 protein expression after treating cells with rambutan extracts according to Example 1 (NADES solvent) and Comparative Example 1 (50% ethanol aqueous solvent).

[0127] Figure 8 is a graph showing the change in MMP-13 protein expression after treating cells with rambutan extracts according to Example 1 (NADES solvent) and Comparative Example 1 (50% ethanol aqueous solvent).

[0128] FIG. 9 is an image for visually analyzing skin reaction and damage improvement after using Examples 2-1 to 2-3, Comparative Example 2, and an untreated group on human skin stimulated from the outside (chemical) for 3 or 14 days.

[0129] Figure 10 is a graph showing the transepidermal water loss (TEWL) data after using Examples 2-1 to 2-3, Comparative Example 2, and an untreated group on human skin stimulated by an external (chemical) source for 3 or 14 days.

[0130] Figure 11 is a graph showing the skin redness (EI) data after using Examples 2-1 to 2-3, Comparative Example 2, and an untreated group on human skin stimulated by an external (chemical) source for 3 or 14 days.

[0131] FIGS. 12a and 12b are graphs showing the survey evaluations of the subjects after using Examples 2-1 to 2-3, Comparative Example 2, and the untreated group on human skin stimulated from the outside (chemical) for 14 days.

[0132] Figure 13 is an image showing adverse skin reactions of subjects after using Examples 2-1 to 2-3, Comparative Example 2, and the untreated group on human skin stimulated from the outside (chemical) for 14 days.

[0133] Examples are provided to aid in understanding the present invention. The following examples are provided merely to facilitate a better understanding of the invention, and the scope of the invention is not limited by these examples.

[0134] <Example>

[0135] Example 1. Preparation of Rambutan (Nephelium lappacem) Extract Using Natural Eutectic Solvents (NADES)

[0136] After purchasing Vietnamese rambutan fruits, the flesh was removed and only the peel was taken. The peel was washed and completely dried, then finely ground into powder using a mixer.

[0137] Meanwhile, 5 g of distilled water (DW) was added to 2.18 g of betaine and 2.83 g of propanediol and dissolved in a sonicator at 80°C for 3 hours to prepare an extraction solvent containing natural deep eutectic solvents (NADES). At this time, the extraction solvent had a weight ratio of NADES:DW = 50:50, and the NADES contained in the extraction solvent had a molar ratio of Betaine:Propandiol = 1:2.

[0138] 100 mg of rambutan peel powder was added to the prepared extraction solvent and stirred at 80°C for 1 hour at a speed of 500 rpm. Afterward, the remaining raw material was removed using a 0.45 μm filter to prepare the rambutan extract.

[0139] Example 2. Composition comprising rambutan extract extracted with NADES

[0140] Example 2-1. Essence composition containing 0.2 wt% rambutan extract

[0141] An essence composition containing 0.2 wt% of the rambutan extract of Example 1 was prepared by mixing the ingredients listed in Table 1 below so that each ingredient has the content as shown in Table 1 relative to the total weight of the composition.

[0142] Example 2-2. Essence composition containing 0.5 wt% rambutan extract

[0143] An essence composition was prepared in the same manner as in Example 2-1, except that the content of rambutan extract was adjusted as shown in Table 1 below.

[0144] Example 2-3. Essence composition containing 1 wt% rambutan extract

[0145] An essence composition was prepared in the same manner as in Example 2-1, except that the content of rambutan extract was adjusted as shown in Table 1 below.

[0146] Comparative Example 1. Preparation of Rambutan (Nephelium lappacem) extract using a 50% aqueous ethanol solution

[0147] A rambutan extract according to Comparative Example 1 was prepared in the same manner as in Example 1, except that a 50% aqueous ethanol solution (50% EtOH) was used instead of a natural eutectic solvent (NADES).

[0148] Comparative Example 2. Essence composition not containing rambutan extract

[0149] An essence composition was prepared in the same manner as in Example 2-1, except that it did not contain 0.2 wt% of rambutan extract, as shown in Table 1 below.

[0150] Table 1 below shows the components of the essence compositions according to Examples 2-1 to 2-3 and Comparative Example 2 and the content of each component (Mixed component A is a mixture of 7 components mixed at 2 wt% each).

[0151] Ingredient Content (wt%) Example 2-1 Example 2-2 Example 2-3 Comparative Example 2 Purified Water Remaining Remaining Remaining Remaining Rambutane Extract 0.20.5 10 Acrylate / C10-30 Alkyl Acrylate Crosspolymer 0.20.20.20.2 Ammonium Acryloyl Dimethyl Taurate / VP Copolymer 0.50.50.50.5 Glycerin 1.51.51.51.5 Butylene Glycol 1.51.51.51.5 Disodium EDTA 0.050.050.050.0 5 Sodium Gluconate 1.11.11.11.1 Ethylhexylglycerin 0.05 0.05 0.05 0.05 Polyglyceryl-10 Laurate 0.20 0.20 0.2 Sorbitan Stearate 0.50 0.50 0.5 Cetearyl Oliveate 0.50 0.50 0.5 Sorbitan Oliveate 0.50 0.50 0.5 Coconut Palm Oil 0.10 0.10 0.1 Carrot Extract (as MCT Extraction) 0.10.10.10.1 Dimethicone 2.52.52.52.5 Isopropyl Myristate 0.50.50.50.5 Caprylic / Capric Triglyceride 0.50.50.50.5 Dimethicone / Vinyl Dimethicone Crosspolymer 0.50.50.50.5 Dimethicone 0.50.50.50.5 Arginine 0.50.50.50.5 Mixed Ingredients A Hydroxyacetophenone 2222 Dipropylene Glycol 2222 Caprylyl Glycol 2222 Dipotassium Glycyrrhizate 2222 Glycerin 2222 Butylene Glycol 2222 Purified Water 2222

[0152] Example 3. Analysis of Skin Inflammation Alleviation by Rambutan Extract

[0153] (1) Analysis of changes in inflammatory cytokine expression after LPS stimulation induction

[0154] 1 × 10⁶ cells (HaCaT cells) in a 6-well plate 5Cells were cultured at a concentration of cells / mL for 24 hours, and after inducing LPS stimulation, the cell culture medium was collected 18 hours after treatment with rambutan extract extracted with NADES (Example 1) and rambutan extract extracted with 50% ethanol (Comparative Example 1), respectively, and the production of the inflammatory cytokines TNF-α and IL-1α was measured; the results are shown in Figures 1 and 2 (CON: control group, LPS: LPS-stimulated cell group, 50% EtOH: test group treated with 50% ethanol aqueous rambutan extract of Comparative Example 1 to LPS-stimulated cells, NADES: test group treated with natural eutectic solvent rambutan extract of Example 1 to LPS-stimulated cells).

[0155] According to Figures 1 and 2, compared to cells treated with rambutan extract extracted with 50% ethanol (Comparative Example 1), the production of inflammatory cytokines TNF-α and IL-1α was found to be further reduced in cells treated with rambutan extract extracted with NADES (Example 1).

[0156] Therefore, it can be seen that the rambutan extract extracted with NADES according to the present invention (Example 1) has a superior effect in alleviating skin inflammation.

[0157] (2) Analysis of changes in inflammatory cytokine expression after UV stimulation induction

[0158] 1 × 10⁶ cells (HaCaT cells) in a 6-well plate 5Cells were cultured at a concentration of cells / mL for 24 hours, and UV-exposed cells were treated with rambutan extract extracted with NADES (Example 1) and rambutan extract extracted with 50% ethanol (Comparative Example 1), respectively. After 18 hours, the cell culture medium was collected, and the production of the inflammatory cytokines TNF-α and IL-1α was measured. The results are shown in Figures 3 and 4 (CON: control group, UV: UV-exposed cell group, 50% EtOH: test group treated with the 50% ethanol aqueous rambutan extract of Comparative Example 1 on UV-exposed cells, NADES: test group treated with the natural eutectic solvent rambutan extract of Example 1 on UV-exposed cells).

[0159] According to Figures 3 and 4, compared to cells treated with rambutan extract extracted with 50% ethanol (Comparative Example 1), the production of inflammatory cytokines TNF-α and IL-1α was found to be further reduced in cells treated with rambutan extract extracted with NADES (Example 1).

[0160] Therefore, it can be seen that the rambutan extract extracted with NADES according to the present invention (Example 1) has a superior effect in alleviating skin inflammation.

[0161] Example 4. Analysis of the whitening efficacy of rambutan extract

[0162] (1) Analysis of melanin synthesis inhibition by α-MSH stimulation

[0163] B16F10 cells were treated with α-MSH and the rambutan extracts according to Example 1 and Comparative Example 1, respectively, and cultured for 5 days. The cells were collected and washed twice with phosphate-buffered saline (PBS). The cells were then treated with a 1N NaOH solution containing 10% dimethylsulfoxide (DMSO, Ameresco, Solon, Ohio, USA) and reacted at 80°C for 1 hour. Melanin quantification was performed by measuring the absorbance of each sample at 475 nm using an ELISA reader (Molecular Devices, Sunnyvale, CA, USA) with synthetic melanin to construct a standard curve for each concentration, and then determining the melanin concentration of each sample from the constructed standard curve; the results are shown in Fig. 5 (CON: control group, α-MSH: α-MSH treated group, 50% EtOH: test group treated with α-MSH-treated cells and the 50% ethanol aqueous rambutan extract of Comparative Example 1, NADES: test group treated with α-MSH-treated cells and the natural eutectic solvent rambutan extract of Example 1).

[0164] According to Figure 5, the melanin concentration was found to be lower in cells treated with rambutan extract extracted with NADES (Example 1) compared to cells treated with rambutan extract extracted with 50% ethanol (Comparative Example 1).

[0165] Accordingly, it was confirmed that the rambutan extract extracted with NADES according to the present invention (Example 1) has significantly superior efficacy in inhibiting melanin synthesis caused by α-MSH stimulation.

[0166] Example 5. Analysis of the anti-aging efficacy of rambutan extract

[0167] (1) Analysis of changes in MMP-1, MMP-9, and MMP-13 protein expression

[0168] NIH3T3 cells were cultured under standard culture conditions (37°C, 5% CO2) and treated with TNF-α for 24 hours to induce an inflammatory response, then treated with each of the rambutan extracts according to Example 1 and Comparative Example 1 for 12 hours, and the changes in the expression of MMP-1, MMP-9, and MMP-13 proteins were evaluated using Western blot and are shown in Figures 6 to 8.

[0169] According to Figures 6 to 8, it was confirmed that the expression of MMP-1, MMP-9, and MMP-13 induced by TNF-α was significantly reduced in cells treated with rambutan extract extracted with NADES (Example 1) compared to cells treated with rambutan extract extracted with 50% ethanol (Comparative Example 1).

[0170] Accordingly, it was confirmed that the rambutan extract extracted with NADES according to the present invention (Example 1) has an excellent effect in inhibiting the expression of MMP-1, MMP-9, and MMP-13, and has excellent anti-aging efficacy.

[0171] Example 6. Evaluation Test of Skin Condition Improvement / Alleviation Effects Due to Chemical Irritation

[0172] The barrier improvement and redness alleviation effects on human skin stimulated by an external (chemical) source for Examples 2-1 to 2-3 and Comparative Example 2 were evaluated, and the evaluation method is as shown in Table 2 below.

[0173] a. Subjects: 21 female volunteers aged 26 to 53 (mean 41.8 ± 7.8) b. Measurement Methods b-1. Measurement Site: Forearm (randomly assigned to left or right) b-2. Time Points: Before and after SLS patch application, after 3 days of product use, and after 14 days of product use b-3. Evaluation Items: Instrumental measurements (Transepidermal Water Loss (TEWL) and Skin Erythrin (EI)), questionnaire evaluation by subjects, and skin safety evaluation

[0174] (1) Visual evaluation analysis of skin reaction and damage (redness) improvement

[0175] Figure 9 shows the results of a visual evaluation of the skin reaction and damage improvement effects caused by chemical irritation by the compositions containing the rambutan extracts of Examples 2-1 to 2-3 of the present invention. Table 4 below summarizes the degree of skin improvement after using the essence containing the compositions containing the rambutan extracts of Examples 2-1 to 2-3 of the present invention as visual evaluation scores. These visual evaluation scores were measured and presented according to the criteria in Table 3 below.

[0176] Grade | Description 0. No recovery at all Erythema, scaling, fissures, etc. show no reduction whatsoever 1. Slight recovery Scale and fissures remain visible, but erythema is slightly reduced 2. Slight recovery Scale and fissures remain the same or appear increased, but erythema is reduced 3. Recovery All symptoms, including erythema, scaling, and fissures, show a significant reduction 4. Complete recovery All symptoms, including erythema, scaling, and fissures, are completely eliminated

[0177] According to FIG. 9 and Table 4 of the present invention, the skin reaction showed that stratum corneum damage of 'Slight erythema, either spotty or diffuse' was homogeneously induced in all groups after SLS patch application (p>0.05).

[0178] As a result of the visual evaluation analysis, at the time of evaluation after 14 days, the visual evaluation score of the degree of skin improvement was found to have significantly increased (improved) in the test group treated with the composition containing the rambutan extract of Examples 2-1 to 2-3 (p<0.05).

[0179] Classification Degree of Skin Improvement Visual Evaluation Score Example 2-12.05 ± 0.69 Example 2-22.52 ± 0.62 Example 2-33.48 ± 0.58 Control Group 1.43 ± 0.58 Untreated Group 1.10 ± 0.56

[0180] (2) Transepidermal Water Loss (TEWL) Analysis

[0181] The results of the analysis of transepidermal water loss (TEWL) are shown in Figure 10.

[0182] According to Figure 10, the maximum improvement rate of transepidermal water loss (TEWL) was found to be significantly higher in the treatment group containing the rambutan extract of Examples 2-1 to 2-3 compared to the treatment group of Comparative Example 2 and the untreated group at the time after 14 days of use.

[0183] In particular, the transepidermal water loss (TEWL) improvement rate of the treatment group containing the rambutan extract of Examples 2-3 was found to be the best.

[0184] (3) Skin redness (E.1) Analysis

[0185] The results of the analysis of the degree of improvement in skin redness are as shown in Figure 11.

[0186] According to FIG. 11, the maximum improvement rate of skin redness (E.1) was found to be significantly higher in the treatment group containing the rambutan extract of Examples 2-1 to 2-3 compared to the treatment group of Comparative Example 2 and the untreated group at the time of 14 days of use. In particular, the improvement rate of skin redness (E.1) was found to be the best in the treatment group containing the rambutan extract of Example 2-3.

[0187] (4) Survey evaluation by research subjects

[0188] The skin-improving efficacy of a composition containing rambutan extract extracted with NADES of the present invention was evaluated through a survey of research subjects, and the results are as shown in FIGS. 12a to 12b.

[0189] According to Fig. 12a, regarding the efficacy of the product after 14 days of use, it was confirmed that about 71 to 81% of the subjects in the treatment group of Example 2-1, about 90 to 100% of the subjects in the treatment group of Example 2-2, and all subjects (100%) in the treatment group of Example 2-3 were satisfied with the product for all items.

[0190] In addition, according to Fig. 12b, regarding the usability of the product, about 67 to 86% of the subjects in the treatment group of Example 2-1, about 81 to 95% of the subjects in the treatment group of Example 2-2, and about 95 to 100% of the subjects in the treatment group of Example 3 expressed satisfaction with the product, and it was confirmed that satisfaction tended to increase as the concentration of rambutan extract included in the composition increased.

[0191] Accordingly, it was confirmed that the composition containing rambutan extract extracted with the natural eutectic solvent (NADES) of the present invention has the effect of improving skin damage caused by external stimuli, such as improving the damaged skin surface, improving the skin barrier, soothing redness, absorption, and soothing sensitivity. Furthermore, it was found that the higher the concentration of rambutan extract included in the composition, the greater the improvement in skin damage caused by external stimuli, and the satisfaction regarding absorption, moisturizing power, and mildness is excellent.

[0192] (5) Skin safety evaluation

[0193] The stability of a composition containing rambutan extract extracted with NADES of the present invention was evaluated through a survey of study subjects, and the results are as shown in Fig. 13.

[0194] According to Figure 13, no adverse skin reactions were observed in any of the subjects during the study period, confirming that it is safe for the skin.

[0195] The specification omits detailed descriptions of matters that can be sufficiently recognized and inferred by those skilled in the art of the present invention, and various modifications are possible within the scope of not altering the technical concept or essential configurations of the present invention, in addition to the specific examples described in this specification. Accordingly, the present invention may be implemented in a manner different from that specifically described and exemplified in this specification, and this is a matter that can be understood by those skilled in the art.

Claims

1. A cosmetic composition for improving skin condition comprising a Rambutan (Nephelium lappaceum) extract extracted with an extraction solvent including Natural Deep Eutectic Solvents (NADES).

2. In Paragraph 1, A cosmetic composition comprising one or more selected from the group consisting of betaine, L-proline, sucrose, propanediol, glycerin, xylitol, and sorbitol, wherein the above natural eutectic solvent comprises betaine, L-proline, sucrose, propanediol, glycerin, xylitol, and sorbitol.

3. In Paragraph 1, The above natural eutectic solvent (NADES) comprises betaine and propanediol, and the molar ratio of betaine to propanediol is 1:1 to 1:4, a cosmetic composition.

4. In Paragraph 1, A cosmetic composition wherein the above extraction solvent further comprises water.

5. In Paragraph 1, With respect to 100 parts by weight of the above extraction solvent, A cosmetic composition comprising 100 parts by weight of the above-mentioned natural eutectic solvent.

6. In Paragraph 1, With respect to 100 parts by weight of the above extraction solvent, A cosmetic composition comprising 40 to 90 parts by weight of the above natural eutectic solvent (NADES) and 10 to 60 parts by weight of water.

7. In Paragraph 1, With respect to the total weight of the above cosmetic composition, A cosmetic composition comprising 0.01 to 20 weight% of the above rambutan extract.

8. In Paragraph 1, A cosmetic composition wherein the improvement of the above skin condition is one or more selected from the group consisting of skin moisturization, redness relief, itching relief, anti-inflammatory, skin irritation relief, skin barrier improvement, skin soothing, skin whitening, and skin aging improvement.

9. A pharmaceutical composition for the prevention or treatment of inflammatory skin diseases comprising a rambutan (Nephelium lappaceum) extract extracted with an extraction solvent including natural deep eutectic solvents (NADES).

10. In Paragraph 9, A pharmaceutical composition comprising one or more selected from the group consisting of betaine, L-proline, sucrose, propanediol, glycerin, xylitol, and sorbitol, wherein the above natural eutectic solvent comprises betaine, L-proline, sucrose, propanediol, glycerin, xylitol, and sorbitol.

11. In Paragraph 10, A pharmaceutical composition wherein the above natural eutectic solvent comprises betaine and propanediol, and the molar ratio of betaine to propanediol is 1:1 to 1:

4.

12. In Paragraph 9, A pharmaceutical composition wherein the above extraction solvent further comprises water.

13. In Paragraph 9, With respect to 100 parts by weight of the above extraction solvent, A pharmaceutical composition comprising 100 parts by weight of the above-mentioned natural eutectic solvent.

14. In Paragraph 9, With respect to 100 parts by weight of the above extraction solvent, A pharmaceutical composition comprising 40 to 90 parts by weight of the above natural eutectic solvent (NADES) and 10 to 60 parts by weight of water.

15. In Paragraph 9, With respect to the total weight of the above pharmaceutical composition, A pharmaceutical composition comprising 0.01 to 20 weight% of the above rambutan extract.

16. In Paragraph 9, A pharmaceutical composition wherein the above-mentioned inflammatory skin disease comprises one or more selected from the group consisting of pruritus, atopic dermatitis, chronic recurrent dermatitis, contact dermatitis, seborrheic dermatitis, neurodermatitis, xerosis, erythema, inflammatory dermatitis, psoriasis, urticaria, pruritus, dry eczema, lupus erythematosus, and atopic diseases.

17. A food composition for improving skin condition comprising a rambutan (Nephelium lappaceum) extract extracted with an extraction solvent including natural deep eutectic solvents (NADES).

18. A food composition for the prevention or improvement of inflammatory skin diseases comprising rambutan extract extracted with an extraction solvent including natural deep eutectic solvents (NADES).

19. A method for preparing a rambutan extract comprising the step of extracting rambutan (Nephelium lappaceum) with an extraction solvent containing natural deep eutectic solvents (NADES).

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