Composition and method for controlling plant diseases

Abstract

The purpose of the present invention is to provide a composition that contains, as an effective microbe, a microorganism having the effect of inhibiting the onset of plant diseases, that is usable as a biotic pesticide, and that is for use in controlling plant diseases.　The composition is for use in controlling plant diseases, and contains, as an active ingredient, at least one selected from the group consisting of cells of a Brevibacillus formosus strain NBRC 15716 or a mutant strain thereof, or a culture thereof, and cells of a Brevibacillus brevis strain NBRC 110488 or a mutant strain thereof, or a culture thereof.

Description

Composition and method for controlling plant diseases

[0001] The present invention relates to a composition for use in controlling plant diseases and a method for controlling plant diseases. More specifically, the present invention relates to a composition for use in controlling plant diseases, comprising as an active ingredient at least one selected from the group consisting of the cells or cultures thereof of Brevibacillus formosus NBRC 15716 strain or its mutant strain and Brevibacillus brevis NBRC 110488 strain or its mutant strain or its culture, and a method for controlling plant diseases, comprising applying the above composition to a target plant.

[0002] The primary method of controlling plant diseases has been the use of chemical pesticides, which have enabled stable crop production to date. However, in recent years, the environmental impact of continuous use of chemical pesticides and the emergence of resistant strains have made it difficult to adequately control diseases with existing chemical pesticides, and the problem of difficult-to-control diseases such as bacterial diseases has become significant. Therefore, as an alternative to chemical pesticides, biological control technologies using microorganisms isolated from nature have attracted attention, and several microbial pesticides have been commercialized. However, existing microbial pesticides have drawbacks compared to chemical pesticides, such as unstable efficacy and a limited range of applicable diseases. Given this situation, there is a need for novel microbial pesticides that have new applicable diseases and exhibit stable control efficacy.

[0003] As plant disease control agents using microorganisms, Pseudomonas fluorescens, non-pathogenic Erwinia carotovora, Trichoderma atroviride, Bacillus simplex, and Bacillus subtilis are registered and used as microbial pesticides.

[0004] Brevibacillus is a type of aerobic, Gram-positive, endospore-forming rod-shaped bacterium commonly found in environments such as soil, water, and air. Patent Document 1 discloses a biological control agent using a strain of Brevibacillus laterosporus that has insecticidal activity against lepidopteran and diptera species.

[0005] On the other hand, as plant disease control methods using Brevibacillus fungi, Non-Patent Document 1 discloses a method for controlling powdery mildew in grapes using Brevibacillus brevis Strain CP-1, and Non-Patent Document 2 discloses a method for controlling potato scab using Brevibacillus laterosporus BL12.

[0006] Furthermore, Patent Document 2 discloses a method for controlling tobacco wilt disease, caused by Ralstonia solanacearum, a type of plant pathogenic bacterium, using Brevibacillus brevis strain NJL-25 and Bacillus cereus strain NJL-14.

[0007] Furthermore, Patent Document 3 discloses a method for suppressing the growth of plant pathogenic filamentous fungi of the genera Fusarium, Phytophthora, Pythium, Rhizoctonia, and Sclerotinia using Brevibacillus laterosporus strain CM-3 or Brevibacillus laterosporus strain CM-33. In addition, Patent Document 4 discloses a method for controlling late blight on bell peppers using cultures of Bacillus brevis NF2 and Paenibacillus polymyxa DL.

[0008] However, in all of the literature mentioned above, the methods were only effective in controlling diseases in specific (a small number of) plants, and the effectiveness in controlling plant diseases was not entirely satisfactory. As a result, a practical plant disease control agent using Brevibacillus fungi as the active ingredient has not yet been put into practical use.

[0009] Japanese Patent Publication No. 2015-535684, US Patent No. 8476057, US Patent No. 10154670, Chinese Patent No. 109169712

[0010] Meltem Avan, Recep Kotan, Eslam Mohamed Albastawisi, Nazenin Eftekhari,. Biological Control of Grapevine Powdery Mildew Disease by Using Brevibacillus brevis Strain CP-1, 13 October 2023, Volume 65, pages 2125-2133, Erwerbs-ObstbauCuiping Li, Wencong Shi, Di Wu, Renmao Tian, ​​Bing Wang, Rongshan Lin, Bo Zhou, Zheng Gao,. Biocontrol of potato common scab by Brevibacillus laterosporus BL12 is related to the reduction of pathogen and changes in soil bacterial community, February 2021, Volume 153, Biological Control

[0011] The object of the present invention is to provide a composition for use in controlling plant diseases that contains a microorganism having a disease-suppressing effect on plant diseases as an effective fungicide, and that can be used as a biopesticide.

[0012] In view of the above-mentioned problems, the present inventors have conducted extensive research and have succeeded in discovering that at least one species selected from the group consisting of the fungal cells or cultures of Brevibacillus formosus strain NBRC 15716 or its mutant strain, and the fungal cells or cultures of Brevibacillus brevis strain NBRC 110488 or its mutant strain, has an effect of suppressing the onset of plant diseases. A composition containing at least one selected from the group consisting of the cells or cultures of Brevibacillus formosus strain NBRC 15716 or its mutant strain and Brevibacillus brevis strain NBRC 110488 or its mutant strain as an active ingredient has a disease-suppressing effect against bacteria, basidiomycetes, ascomycetes, imperfect fungi, oomycetes, or zygomycetes that cause plant diseases, and among these, it has a particularly noteworthy disease-suppressing effect against oomycetes.

[0013] Specific embodiments of the present invention are as follows: [1] A composition for use in controlling plant diseases, comprising, as an active ingredient, at least one selected from the group consisting of cells or cultures thereof of Brevibacillus formosus strain NBRC 15716 or a mutant thereof, and cells or cultures thereof of Brevibacillus brevis strain NBRC 110488 or a mutant thereof. [2] The composition according to [1], wherein the plant disease is a plant disease caused by bacteria, basidiomycetes, ascomycetes, imperfect fungi, oomycetes, or zygomycetes. [3] The composition according to [1] or [2], wherein the plant disease is a plant disease caused by oomycetes. [4] The composition according to any one of [1] to [3], comprising the cell or culture of Brevibacillus formosus strain NBRC 15716 or a mutant strain thereof as an active ingredient, wherein the plant disease is a plant disease caused by oomycetes. [5] The composition according to any one of [1] to [3], comprising the cell or culture of Brevibacillus brevis strain NBRC 110488 or a mutant strain thereof as an active ingredient, wherein the plant disease is a plant disease caused by oomycetes. [6] A method for controlling plant diseases, comprising applying the composition according to any one of [1] to [5] to a target plant.

[0014] [7] The composition according to any one of [1] to [5], wherein at least one selected from the group consisting of cells or cultures thereof of Brevibacillus formosus NBRC 15716 strain or a mutant thereof and cells or cultures thereof of Brevibacillus brevis NBRC 110488 strain or a mutant thereof is cultured in a medium using sucrose as a carbon source. [8] The composition according to any one of [1] to [5] and [7], wherein at least one selected from the group consisting of cells or cultures thereof of Brevibacillus formosus NBRC 15716 strain or a mutant thereof and cells or cultures thereof of Brevibacillus brevis NBRC 110488 strain or a mutant thereof is cultured in a medium using soybean meal or soybean flour as a nitrogen source. [9] The composition according to any one of [1] to [5], [7], and [8], wherein the target plant is tomato, eggplant, bell pepper, chili pepper, cucumber, bitter melon, watermelon, pumpkin, melon, onion, leek, lettuce, cabbage, radish, spinach, Chinese cabbage, turnip, broccoli, cauliflower, sugar beet, asparagus, ginger, myoga, potato, taro, adzuki bean, soybean, broad bean, strawberry, grape, pineapple, almond, chrysanthemum, gerbera, tulip, lily, or carnation.

[10] The composition according to any one of [1] to [5] and [7] to [9], wherein the plant disease is tomato late blight, grape downy mildew, cucumber powdery mildew, rice blast, wheat rust, cucumber brown spot, Chinese cabbage black spot, cucumber gray mold, cucumber bacterial leaf spot, or Chinese cabbage soft rot.

[11] The composition according to any one of [1] to [5] and [7] to [9], wherein the plant disease is tomato late blight, grape downy mildew, cucumber powdery mildew, rice blast, wheat rust, cucumber brown spot, Chinese cabbage black spot, cucumber gray mold, cucumber bacterial leaf spot, Chinese cabbage soft rot, cucumber downy mildew, or onion soft rot.

[12] A method for controlling plant diseases, comprising applying a composition described in any of [7] to

[11] to a target plant.

[13] The use of a composition for controlling plant diseases, wherein the composition is a composition described in any of [1] to [5] and [7] to

[11] .

[0015] The Brevibacillus formosus NBRC 15716 strain or the Brevibacillus brevis NBRC 110488 strain used in this invention can suppress the occurrence of a wide range of plant diseases by placing their culture (including live cells) or live cells isolated from the culture on plant bodies such as roots, stems, leaves, seeds, and fruits, or in the cultivation soil.

[0016] Figure 1 shows the sequence of 16S ribosomal DNA (rDNA) of Brevibacillus formosus NBRC 15716 (Sequence ID 1). Figure 2 shows the sequence of 16S rDNA of Brevibacillus brevis NBRC 110488 (Sequence ID 2).

[0017] In this specification, "control" means preventing, treating, suppressing, or eliminating plant diseases. In this specification, "control method" means a method for preventing, treating, suppressing, or eliminating plant diseases.

[0018] (Composition for use in the control of plant diseases) The inventors screened for microorganisms with a very broad antimicrobial spectrum against various plant diseases. As a result, they obtained the useful finding that Brevibacillus formosus NBRC strain 15716 or Brevibacillus brevis NBRC strain 110488, registered with the National Institute of Technology and Evaluation (NITE) Biological Genetic Resources Division (NBRC), exhibit broad antimicrobial activity against various plant pathogens. Brevibacillus formosus NBRC strain 15716 or Brevibacillus brevis NBRC strain 110488 showed particularly high antimicrobial activity against oomycetes among various plant pathogens.

[0019] Based on sequence analysis of the 16S rDNA gene (Sequence ID 1 (Figure 1)), strain Brevibacillus formosus NBRC 15716 was deposited as NBRC15716 with the National Institute of Technology and Evaluation (NITE) Biotechnology Center (NBRC) on June 7, 1994.

[0020] Furthermore, Brevibacillus formosus strain NBRC 15716 has the following bacteriological characteristics: (1) Morphological properties: Shape: Rod-shaped Size: Width 0.7–0.9 μm, Length 2.0–3.5 μm Motility: + Presence or absence of spores: + (2) Culture properties Medium: SCD agar (30°C) Shape: Circular Color: Creamy white (3) Physiological properties Gram staining: -

[0021] In one embodiment of the present invention, a mutant strain of Brevibacillus formosus NBRC 15716 has 16S rDNA represented by a nucleotide sequence that has 80% or more, 85% or more, 90% or more, 91% or more, 92% or more, 93% or more, 94% or more, 95% or more, 96% or more, 97% or more, 98% or more, 99% or more, or 99.5% or more identity with the nucleotide sequence of Sequence ID No. 1. In one embodiment of the present invention, a mutant strain of Brevibacillus formosus NBRC 15716 has 16S rDNA represented by a nucleotide sequence that has 99.5% or more identity with the nucleotide sequence of Sequence ID No. 1, and also possesses the bacteriological characteristics of the above-mentioned Brevibacillus formosus NBRC 15716 strain. In one aspect of the present invention, the mutant strain of Brevibacillus formosus NBRC 15716 is a mutant strain derived from Brevibacillus formosus NBRC 15716.

[0022] Based on sequence analysis of the 16S rDNA gene (SEQ ID NO: 2 (Figure 2)), strain Brevibacillus brevis NBRC 110488 was deposited with the National Institute of Technology and Evaluation (NBRC) Biotechnology Center (NBRC) on July 15, 2014, as NBRC110488.

[0023] Furthermore, Brevibacillus brevis strain NBRC 110488 has the following bacteriological characteristics: (1) Morphological properties: Form: Rod-shaped Size: Width 0.7-0.9 μm, Length 2.0-4.0 μm Motility: + Presence or absence of spores: + (2) Culture properties Medium: SCD agar (30°C) Shape: Circular Color: Creamy white (3) Physiological properties Gram staining: -

[0024] In one embodiment of the present invention, a mutant strain of Brevibacillus brevis NBRC 110488 has 16S rDNA represented by a nucleotide sequence that has 80% or more, 85% or more, 90% or more, 91% or more, 92% or more, 93% or more, 94% or more, 95% or more, 96% or more, 97% or more, 98% or more, 99% or more, or 99.5% or more identity with the nucleotide sequence of Sequence ID No. 2. In one embodiment of the present invention, a mutant strain of Brevibacillus brevis NBRC 110488 has 16S rDNA represented by a nucleotide sequence that has 99.5% or more identity with the nucleotide sequence of Sequence ID No. 2, and also possesses the bacteriological characteristics of the above-mentioned Brevibacillus brevis NBRC 110488 strain. The mutant strain of Brevibacillus brevis NBRC 110488 is a mutant strain derived from Brevibacillus brevis NBRC 110488.

[0025] Mutant strains derived from Brevibacillus formosus NBRC 15716 or Brevibacillus brevis NBRC 110488 include, for example, naturally occurring mutants, mutants treated with ultraviolet irradiation, X-ray irradiation, or mutagenic agents (e.g., N-methyl-N-nitro-N-nitrosoguanidine), and polyploid strains. Specifically, mutant strains that maintain 99.5% or more identity with 16S rDNA and possess the same bacteriological characteristics as the parent plant are suitably usable. These mutant strains are included in the range of mutant strains in one aspect of the present invention as long as they retain their plant disease control activity. Here, retaining plant disease control activity means that this activity is 80% or more compared to the parent plant.

[0026] In one embodiment of the present invention, the Brevibacillus formosus NBRC 15716 strain or the Brevibacillus brevis NBRC 110488 strain used as the active ingredient of the composition can be grown by known means such as static culture on solid media or liquid culture, and the type of medium and culture conditions that can be used are not particularly limited as long as the bacteria can survive and grow. For example, in addition to general media such as meat extract medium, media containing glucose, peptone, and yeast extract can be used. In addition to liquid media, solid media such as agar-containing slant media and plate media may also be used.

[0027] Anything that the above-mentioned strains can utilize can be used as a carbon source for the culture medium. Specifically, in addition to sugars such as glucose, galactose, lactose, sucrose, maltose, carboxymethylcellulose (CMC), powdered cellulose, brown rice, oatmeal, malt extract, molasses, corn syrup, and starch hydrolysates, various synthetic or natural carbon sources that can be utilized by Brevibacillus formosus NBRC 15716 strain or Brevibacillus brevis NBRC 110488 strain can be used.

[0028] In particular, using sucrose as a carbon source in the culture medium when culturing Brevibacillus formosus strain NBRC 15716 or Brevibacillus brevis strain NBRC 110488 can demonstrate high antibacterial activity against various plant pathogenic fungi.

[0029] Similarly, various synthetic or natural products that can be utilized by Brevibacillus formosus NBRC 15716 strain or Brevibacillus brevis NBRC 110488 strain can be used as nitrogen sources for the culture medium, including organic nitrogen-containing substances such as peptone, meat extract, yeast extract, soybean meal, soybean flour, and corn steep liquor.

[0030] In particular, when culturing Brevibacillus formosus strain NBRC 15716 or Brevibacillus brevis strain NBRC 110488, using soybean meal or soybean flour as the nitrogen source in the culture medium can demonstrate high antibacterial activity against various plant pathogenic fungi.

[0031] Furthermore, in accordance with conventional methods for microbial culture, inorganic salts such as sodium chloride and phosphates, metal salts such as calcium, magnesium, and iron, and trace nutrients such as vitamins and amino acids can be added as needed.

[0032] Culturing can be carried out under aerobic conditions such as shaking culture or aeration culture. The culture temperature is 20 to 40°C, preferably 25 to 35°C. The pH is preferably 2 to 10, more preferably 3 to 7. The culture period is preferably 1 to 14 days, more preferably 2 to 6 days.

[0033] In one embodiment of the present invention, a culture containing the cells of Brevibacillus formosus NBRC 15716 strain or Brevibacillus brevis NBRC 110488 strain, used as an active ingredient in the composition, has properties that suppress various plant diseases. In one aspect of the present invention, various plant diseases can be suppressed by placing at least one selected from a culture containing cells of Brevibacillus formosus NBRC 15716 or Brevibacillus brevis NBRC 110488, a processed product such as a mixture of the culture and other components, culture-isolated cells such as cells obtained by centrifugation of the culture or washed cells thereof, a processed product such as a mixture of the culture-isolated cells and other components, and dilutions thereof in liquid or solid form, on plant bodies such as roots, stems, leaves, seeds, and fruits, or in the cultivation soil.

[0034] In one aspect of the present invention, Brevibacillus formosus NBRC 15716 strain or Brevibacillus brevis NBRC 110488 strain used as an active ingredient of a composition can be used as a composition for use in controlling plant diseases, whether in the spore state, vegetative cell state, or a state where spores and vegetative cells coexist. Also, even if culture medium components are mixed while cultured, viable cells can be separated from the above culture by a normal method, such as treatment by membrane separation or centrifugation, and washed as necessary, and the cultured and separated cells themselves or their treated products (mixtures of cultured and separated cells and other components, etc.) can be used as an active ingredient. Furthermore, it can also be used in the form of a dried product obtained by drying the above culture or separated viable cells by a method such as freeze-drying or spray-drying, or a diluted product with these liquids or solids.

[0035] In one aspect of the present invention, depending on the application form, the composition can control plant diseases caused by fungi or bacteria belonging to Oomycetes, Ascomycetes, Basidiomycetes, Zygomycetes, or Deuteromycetes. In particular, plant diseases caused by Oomycetes can be more efficiently controlled.

[0036] In one aspect of the present invention, examples of plant diseases that the composition can control and their causative agents (the causative agents are in parentheses) are given below, but are not limited thereto.

[0037] Sugar beet: Cercospora leaf spot (Cercospora beticola), black root rot (Aphanomyces cochloides), root rot (Thanatephorus cucumeris), leaf rot (Thanatephorus cucumeris), rust (Uromyces betae), leaf blight (Alternaria alternata), powdery mildew (Oidium sp.), leaf spot (Ramularia beticola), damping-off (Aphanomyces cochlioides, Pythium ultimum), southern blight (Sclerotium rolfsii), etc.;

[0038] Peanut: Brown leaf spot (Mycosphaerella arachidis), blotch (Ascochyta sp.), rust (Puccinia arachidis), damping-off (Pythium debaryanum), leaf spot (Alternaria alternata), southern blight (Sclerotium rolfsii), black leaf spot (Mycosphaerella berkeleyi), sclerotinia rot (Sclerotinia miyabeana, Sclerotinia sclerotiorum), southern sclerotinia blight (Botryotinia arachidis), etc.;

[0039] Cucumber: Powdery mildew (Sphaerotheca fuliginea), downy mildew (Pseudoperonospora cubensis), vine blight (Mycosphaerella melonis), vine wilt (Fusarium oxysporum), sclerotinia rot (Sclerotinia sclerotiorum), gray mold (Botrytis cinerea), anthracnose (Colletotrichum orbiculare), black leaf mold (Cladosporium cucumerinum), brown leaf spot (Corynespora cassiicola), damping-off (Pythium debaryanum, Rhizoctonia solani Kuhn), phomopsis root rot (Phomopsis sp.), angular leaf spot (Pseudomonas syringae pv. Lachrymans), etc.;

[0040] Tomato: Botrytis cinerea, leaf mold (Passalora fulva, Cladosporium fulvum), late blight (Phytophthora infestans), gray late blight (Phytophthora capsici), brown rot (Phytophthora nicotianae), half-wilt (Verticillium albo-atrum, Verticillium) dahliae), powdery mildew (Oidium neolycopersici), ring spot disease (Alternaria solani), brown ring spot disease (Corynespora cassiicola), bacterial wilt (Ralstonia solanacearum), bacterial spot disease (Pseudomonas syringae pv. tomato), sooty mold (Pseudocercospora fuligena), etc.;

[0041] Eggplant: Gray mold (Botrytis cinerea), black blight (Corynespora melongenae), powdery mildew (Erysiphe cichoracearum), sooty mold (Mycovellosiella nattrassii), sclerotinia sclerotiorum, verticillium wilt (Verticillium dahliae), brown spot disease (Phomopsis vexans), etc.

[0042] Radish: Powdery mildew (Erysiphe cichoracearum), downy mildew (Peronospora parasitica), black spot disease (Alternaria brassicae), sclerotinia sclerotiorum, clubroot (Plasmodiophora brassicae), root constriction disease (Aphanomyces raphani), anthracnose (Colletotrichum higginsianum), soft rot (Pectobacterium carotovorum), white rust (Albugo macrospora), damping-off disease (Pythium sp.), rot (Pythium ultimum var. ultimum), etc.

[0043] Chinese cabbage: Powdery mildew (Oidium matthiolae), Pythium rot (Pythium aphanidermatum), downy mildew (Hyaloperonospora brassicae), yellowing disease (Verticillium dahliae), Sclerotinia sclerotiorum, black spot disease (Alternaria brassicola), clubroot disease (Plasmodiophora brassicae), blossom end rot (Rhizoctonia solani), anthracnose (Colletotrichum higginsianum), soft rot (Pectobacterium carotovorum), white rust (Albugo macrospora), ring spot disease (Asteromella brassicae), etc.

[0044] Strawberries: Gray mold (Botrytis cinerea), powdery mildew (Sphaerotheca humuli), anthracnose (Colletotrichum acutatum, Colletotrichum fragariae), late blight (Phytophthora cactorum), soft rot (Rhizopus stolonifer), fusarium wilt (Fusarium oxysporum), wilt (Verticillium dahliae), etc.

[0045] Onions: Botrytis allii, Botrytis cinerea, Botrytis squamosa, Pectobacterium carotovorum, Peronospora destructor, Phytophthora porri, Pythium sp., Ciborinia allii) etc;

[0046] Leeks: Soft rot (Pectobacterium carotovorum), downy mildew (Peronospora destructor), leaf blight (Pleospora allii), black rot (Sclerotium cepivorum), rust (Puccinia allii), white spot leaf blight (Botrytis squamosa), etc.

[0047] Cabbage: Clubroot (Plasmodiophora brassicae), soft rot (Erwinia carotovora), black rot (Xanthomonas campestris pv. campestris), bacterial black spot (Pseudomonas syringae pv. maculicola, Pseudomonas syringae pv. alisalensis), downy mildew (Peronospora parasitica), sclerotinia sclerotiorum, sooty mold (Alternaria brassicola), gray mold (Botrytis cinerea), etc.

[0048] Green beans: Sclerotinia sclerotiorum, gray mold (Botrytis cinerea), anthracnose (Colletotrichum lindemuthianum), horn spot (Phaeoisariopsis griseola), powdery mildew (Erysiphe pisi, Sphaerotheca fuliginea), seedling blight (Pythium irregulare, Pythium mamillatum, Pythium myriotylum, Pythium spinosum, Pythium ultimum), etc.

[0049] Apples: Powdery mildew (Podosphaera leucotricha), apple scab (Venturia inaequalis), Monilinia mali, black spot (Mycosphaerella pomi), canker (Valsa mali), leaf spot (Alternaria mali), cedar-apple rust (Gymnosporangium yamadae), ring spot (Botryosphaeria berengeriana), anthracnose (Glomerella cingulata, Colletotrichum acutatum), brown spot (Diplocarpon mali), sooty spot (Zygophiala jamaicensis), sooty blotch (Gloeodes pomigena), purple root rot (Helicobasidium mompa), gray mold (Botrytis cinerea), burn (Erwinia amylovora), etc.

[0050] Japanese apricot: Black spot disease (Cladosporium carpophilum), gray mold (Botrytis cinerea), gray mold (Monilinia mumecola), sooty mold (Peltaster sp.), etc.

[0051] Persimmon: Powdery mildew (Phyllactinia kakicola), anthracnose (Gloeosporium kaki), horn spot leaf spot (Cercospora kaki), round leaf spot leaf spot (Mycosphaerella nawae), etc.

[0052] Peach: Gray mold (Monilinia fructicola), black spot (Cladosporium carpophilum), Phomopsis rot (Phomopsis sp.), bacterial hole disease (Xanthomonas campestris pv. pruni), leaf curl (Taphrina deformans), anthracnose (Colletotrichum gloeosporioides), etc.

[0053] Almonds: Gray mold (Monilinia laxa), leaf spot (Stigmina carpophila), black spot (Cladosporium carpophilum), leaf blisters (Polystigma rubrum), leaf spot disease (Alternaria alternata), anthracnose (Colletotrichum gloeospoides), etc.

[0054] Cherry blossom: Monilinia fructicola, anthracnose (Colletotrichum acutatum), black spot (Alternaria sp.), young fruit sclerotia (Monilinia kusanoi), brown borer disease (Mycosphaerella cerasella), etc.;

[0055] Grapes: Gray mold (Botrytis cinerea), gray mold (Monilinia fructigena), powdery mildew (Uncinula necator), late blight (Glomerella cingulata, Colletotrichum acutatum), downy mildew (Plasmopara viticola), black rot (Elsinoe ampelina), black spot (Cladosporium viticolum), brown spot (Pseudocercospora vitis), black rot (Guignardia bidwellii), white rot (Coniella castaneicola), white mold (Sclerotium rolfsii), rust (Phakopsora ampelopsidis), white snow disease (Basidiomycota sp.), etc.

[0056] Pears: Gray mold (Botrytis cinerea), gray spot (Monilinia fructicola), black spot (Venturia nashicola), red-oak rust (Gymnosporangium asiaticum), black spot (Alternaria kikuchiana), ring spot (Botryosphaeria berengeriana), powdery mildew (Phyllactinia mali), canker (Phomopsis fukushii), brown spot (Stemphylium vesicarium), anthracnose (Glomerella cingulata), canker (Valsa ceratosperma), etc.

[0057] Tea: Gray mold (Botrytis cinerea), blight (Exobasidium vexans), ring spot (Pestalotiopsis longiseta, P. theae), anthracnose (Colletotrichum theae-sinensis), leaf spot (Mycosphaerella theae), net blight (Exobasidium reticulatum), red blight (Pseudomonas syringae pv. theae), etc.; Citrus: Scab (Elsinoe fawcettii), blue mold (Penicillium italicum), green mold (Penicillium digitatum), gray mold (Botrytis cinerea), black spot (Diaporthe citri), canker (Xanthomonas campestris pv. Citri), powdery mildew (Oidium sp.), late blight (Phytophthora citrophthora), anthracnose (Colletotrichum (fioriniae), etc.

[0058] Wheat: powdery mildew (Erysiphe graminis f.sp.tritici, Blumeria graminis f.sp. tritici), scab (Gibberella zeae), brown rust (Puccinia recondita), brown snow blight (Pythium iwayamai), red snow blight (Monographella nivalis), eyespot (Pseudocercosporella herpotrichoides), leaf blotch (Septoria tritici), glume blotch (Leptosphaeria nodorum), pink snow mold (Typhula incarnata), gray snow mold (Myriosclerotinia borealis), take-all (Gaeumannomyces graminis), ergot (Claviceps purpurea), common bunt (Tilletia caries), loose smut (Ustilago nuda), blast (Pyricularia grisea), etc.;

[0059] Barley: leaf stripe (Pyrenophora graminea), net blotch (Pyrenophora teres), scald (Rhynchosporium secalis), loose smut (Ustilago tritici, U. nuda), etc.; Rice: blast (Pyricularia oryzae), sheath blight (Rhizoctonia solani), bakanae disease (Gibberella fujikuroi), brown spot (Cochliobolus miyabeanus), seedling blight (Pythium graminicola), bacterial blight (Xanthomonas oryzae), seedling blight bacteriosis (Burkholderia plantarii), brown stripe (Acidovorax avenae), false smut (Ustilaginoidea virens), brown rice (Alternaria alternata, Curvularia intermedia), black belly rice (Alternaria padwickii), red rice (Epicoccum purpurascens), etc.;

[0060] Tobacco: Sclerotinia sclerotiorum, powdery mildew (Erysiphe cichoracearum), blight (Phytophthora nicotianae), downy mildew (Pernospora sp.), gray mold (Botrytis cinerea), rot (Rhizoctonia solani), etc.; Tulips: Gray mold (Botrytis cinerea), blight (Phytophthora cactorum), Sclerotinia sclerotiorum, brown spot (Botrytis tuliparum), anthracnose (Colletotrichum fioriniae), etc.

[0061] Sunflowers: Downy mildew (Plasmopara halstedii), Sclerotinia sclerotiorum, Gray mold (Botrytis cinerea), etc.; Bentgrass: Snow mold (Sclerotinia borealis), Large patch (Rhizoctonia solani), Brown patch (Rhizoctonia solani), Dollar spot (Sclerotinia homoeocarpa), Rice blast (Pyricularia sp.), Red blight (Pythium aphanidermatum), Anthracnose (Colletotrichum graminicola), etc.

[0062] Orchardgrass: Powdery mildew (Erysiphe graminis), root rot (Pythium debaryanum, Pythium ultimum), anthracnose (Colletotrichum cereale), etc.; Soybeans: Purple spot (Cercospora kikuchii), downy mildew (Peronospora manshurica), stem blight (Phytophthora sojae), rust (Phakopsora pachyrhizi), sclerotinia sclerotiorum, anthracnose (Colletotrichum truncatum), gray mold (Botrytis cinerea), black rot (Elsinoe glycines), black spot (Diaporthe phaseolorum var. sojae), etc.

[0063] Potatoes: Late blight (Phytophthora infestans), summer blight (Alternaria solani), black spur (Thanatephorus cucumeris), Verticillium albo-atrum, V. dahliae, V. nigrescens, black foot disease (Pectobacterium atrosepticum), soft rot (Pectobacterium carotovorum), etc.

[0064] Bananas: Panama disease (Fusarium oxysporum), Sigatoka disease (Mycosphaerella fijiensis, M. musicola), anthracnose (Colletotrichum musae), etc.; Rapeseed: Sclerotinia sclerotiorum, root rot (Phoma lingam), black spot disease (Alternaria brassicae), etc.;

[0065] Coffee: Rust disease (Hemileia vastatrix), anthracnose (Colletotrichum coffeanum), brown eye disease (Cercospora coffeicola), etc.; Sugarcane: Brown rust disease (Puccinia melanocephala), etc.;

[0066] Corn: Hale spot disease (Gloeocercospora sorghi), rust disease (Puccinia sorghi), southern rust disease (Puccinia polysora), smut disease (Ustilago maydis), sesame leaf spot disease (Cochliobolus heterostrophus), sooty mold disease (Setosphaeria turcica), etc.

[0067] Cotton: susceptible to diseases such as damping-off (Pythium sp.), rust (Phakopsora gossypii), white mold (Mycosphaerella areola), and anthracnose (Glomerella gossypii).

[0068] Among the diseases mentioned above, those caused by oomycetes include tomato blight, cucumber downy mildew, and grape downy mildew. Diseases caused by ascomycetes include cucumber powdery mildew and rice blast. Diseases caused by basidiomycetes include wheat rust. Diseases caused by imperfect fungi include cucumber brown spot, Chinese cabbage black spot, and cucumber gray mold. Diseases caused by bacteria include cucumber bacterial spot, Chinese cabbage soft rot, and onion soft rot.

[0069] The plants targeted by the composition for use in controlling plant diseases in this embodiment are not particularly limited, but include: grains such as rice, wheat, barley, rye, oats, corn, sorghum, millet, foxtail millet, barnyard millet, pearl millet, finger millet, and buckwheat; tubers such as potatoes, sweet potatoes, taro, yams, and konjac; legumes such as soybeans, adzuki beans, kidney beans, peas, broad beans, peanuts, cowpeas, chickpeas, and pigeon peas; and eggplants, tomatoes, bell peppers, chili peppers, cucumbers, melons, and sweet potatoes. Vegetables such as pumpkin, zucchini, white gourd, bottle gourd, winter melon, bitter melon, cabbage, Chinese cabbage, broccoli, cauliflower, radish, turnip, bok choy, komatsuna, mizuna, green onion, onion, chives, garlic, shallots, asparagus, lettuce, burdock, garland chrysanthemum, butterbur, carrot, mitsuba, celery, parsley, strawberry, spinach, okra, shiso, basil, mint, ginger, myoga, etc.; apple, pear, European pear, quince, Chinese quince, cherry, peach, plum, Japanese apricot, etc. Fruit trees such as berries, chestnuts, walnuts, almonds, pecans, grapes, kiwifruit, akebi, persimmons, figs, pomegranates, raspberries, blackberries, blueberries, cranberries, citrus fruits, loquats, olives, bayberries, mangoes, guavas, avocados, dates, coconuts, bananas, pineapples, papayas, passion fruit, acerola, etc.; specialty crops such as cotton, flax, rushes, rapeseed, sunflowers, sesame, oil palm, sugar beets, sugarcane, tea, coffee, cocoa, hops, tobacco, etc.; cosmos, Flowering plants such as morning glories, marigolds, impatiens, baby's breath, sweet peas, chrysanthemums, carnations, tulips, lilies, daffodils, gladiolus, cyclamen, begonias, water lilies, dahlias, roses, cymbidiums, and cattleyas; turfgrasses such as Korean lawn grass, dwarf Korean lawn grass, Zoysia grass, Bermuda grass, bentgrass, fescue, ryegrass, and bluegrass; trees such as cherry trees, azaleas, oaks, zelkova trees, cedar trees, and beech trees; or a combination of two or more of these.Among these, due to their importance as targets for controlling plant diseases caused by oomycetes, okra, sugar beet, spinach, wasabi, turnip, mustard greens, cauliflower, broccoli, cabbage, radish, arugula, Chinese cabbage, komatsuna, rice, bottle gourd, pumpkin, cucumber, white gourd, winter melon, watermelon, loofah, bitter melon, melon, mango, persimmon, gerbera, chrysanthemum, garland chrysanthemum, sunflower Lettuce, avocado, fig, sesame, taro, shiso, ginger, myoga, asparagus, carrot, celery, parsley, chinaberry, sarcandra, buckwheat, pepper, mitsuba, passion fruit, potato, tobacco, chili pepper, tomato, eggplant, bell pepper, carnation, papaya, pineapple, apricot, strawberry, plum, pear, loquat, peach, almond, grape, apple, dori Beans, cockscomb, guava, chestnut, kiwifruit, broad beans, kidney beans, adzuki beans, soybeans, peas, Japanese pepper, coconut, yam, onion, tulip, leek, lily, chives, garlic, shallots, lisianthus, hops, sugarcane, corn, wheat, barley, etc. are preferred, and more preferably, due to their significant impact on crop yield, tomatoes, eggplants, bell peppers, chili peppers, cucumbers, bitter melon, watermelons, pumpkins, melons, onions, leeks, lettuce, cabbage, radishes, spinach, Chinese cabbage, turnips, broccoli, cauliflower, sugar beets, asparagus, ginger, myoga ginger, potatoes, taro, adzuki beans, soybeans, broad beans, strawberries, grapes, pineapples, almonds, chrysanthemums, gerberas, tulips, lilies, carnations, etc.

[0070] In particular, in one aspect of the present invention, a composition for use in controlling plant diseases has a high disease-suppressing effect against plant diseases caused by oomycetes. Specifically, the oomycetes include Albugo candida, Pseudoperonospora cubensis, Pseudoperonospora humuli, Sclerospora graminicola, Sclerophthora macrospora, Sclerophthora rayssiae, Phytophthora infestans, Phytophthora cactorum, Phytophthora capsici, Phytophthora citrophora, Phytophthora syringe, and Phytophthora soja. Examples include, but are not limited to, *Phytophthora sojae*, *Phytophthora nicotiane var. parasitica*, *Phytophthora palmivora*, *Phytophthora phaseoli*, *Phytophthora porri*, *Plasmopara viticola*, *Plasmopara halstedii*, and *Bremia lactucae*.

[0071] In one aspect of the present invention, compositions for use in controlling plant diseases include post-harvest disease control agents for preventing the decay of crops, particularly fruits, during storage after harvest. In one aspect of the present invention, the types of crops to which the post-harvest disease control agent is applied are not limited, but examples include fruits such as strawberries, grapes, figs, citrus fruits, peaches, melons, watermelons, apples, pears, bananas, and pineapples, and vegetables such as cucumbers, tomatoes, Chinese cabbage, cabbage, leeks, onions, carrots, radishes, ginger, bell peppers, eggplants, pumpkins, and bean sprouts. The types of fungi that cause post-harvest diseases are not limited, but examples include Botrytis cinerea, Colletotrichum gloeosporioides, and Alternaria alternata.

[0072] In one aspect of the present invention, a composition for use in controlling plant diseases may use either Brevibacillus formosus NBRC 15716 strain or Brevibacillus brevis NBRC 110488 strain alone, or both strains may be used in combination. Furthermore, each strain's mutants may also be used. The mutant strains of Brevibacillus formosus NBRC 15716 strain or Brevibacillus brevis NBRC 110488 strain are not particularly limited, as long as they are applicable to the composition for use in controlling plant diseases. In one aspect of the present invention, a mutant is one that possesses the microbiological characteristics of the parent strain and has plant disease control activity, and can include naturally occurring mutants, mutants induced by ultraviolet light or chemical mutagens, cell fusion strains, and genetically modified strains.

[0073] In one embodiment of the present invention, a composition for use in controlling plant diseases is provided as a microbial formulation. The composition for use in controlling plant diseases may be used alone as a bacterial cell or culture of Brevibacillus formosus NBRC 15716 strain or Brevibacillus brevis NBRC 110488 strain, or as a formulation diluted with an inert liquid or solid carrier and optionally with surfactants, dispersants, and other auxiliary agents. Specific examples of formulations include granules, powders, wettable powders, suspensions, emulsions, and other dosage forms. In one embodiment of the present invention, the cells or cultures of Brevibacillus formosus strain NBRC 15716 or its mutant strain, and the cells or cultures of Brevibacillus brevis strain NBRC 110488 or its mutant strain may be dried powders.

[0074] Examples of carriers include talc, bentonite, kaolin, clay, diatomaceous earth, white carbon, vermiculite, slaked lime, ammonium sulfate, silica sand, urea, porous solid carriers, and liquid carriers such as water, isopropyl alcohol, methylnaphthalene, xylene, cyclohexanone, and alkylene glycol. Examples of surfactants and dispersants include dinaphthylmethanesulfonates, alcohol sulfates, lignin sulfonates, alkylaryl sulfonates, polyoxyethylene glycol ethers, polyoxyethylene sorbitan monoalkylates, and polyoxyethylene alkylaryl ethers. Examples of auxiliary agents include carboxymethylcellulose, polyethylene glycol, propylene glycol, acacia gum, and xanthan gum, and examples of protective agents include skim milk and pH buffers. In this case, the amount of viable cells or culture of Brevibacillus formosus NBRC 15716 strain or Brevibacillus brevis NBRC 110488 strain, as well as the timing and amount of application, can be appropriately determined in accordance with the case of the viable cells mentioned above. The carrier, surfactant, dispersant, and auxiliary agent can be used individually or in combination of two or more.

[0075] In one embodiment of the present invention, a composition for use in controlling plant diseases may optionally contain active ingredients other than the active ingredient, such as fungicides, insecticides, acaricides, nematicides, snailicides, feeding inhibitors, herbicides, algicides, biopesticides, pheromones, natural fungicides, natural insecticides, fertilizers, etc. Also in one embodiment of the present invention, a composition for use in controlling plant diseases may contain other types of fungal strains along with Brevibacillus formosus NBRC 15716 strain or Brevibacillus brevis NBRC 110488 strain.

[0076] Examples of bactericidal components include iturin A, iturin AL, mycosbutyrin, basilomycin D, basilomycin F, basilomycin Lc, fengisin, prepastatin, fusalicidine, vitertanol, bromconazole, cyproconazole, difenoconazole, diniconazole, enilconazole, epoxyconazole, fluquinconazole, fenbuconazole, flusilazole, flutriafole, hexaconazole, imibenconazole, ipconazole, metconazole, mycrobutanil, penconazole, propiconazole, and p Rothioconazole, simeconazole, triadimefon, triadimenol, tebuconazole, tetraconazole, triticonazole, prochloraz, pefurazoate, imazalil, triflumizole, cyazofamide, benomyl, carbendazim, thiabendazole, fuberidazole, etaboxam, etridiazole, oxpoconazole fumarate, himexazole, azoxystrobin, dimoxystrobin, enestrobrin, fluoxastrobin, kresoxim-methyl, metominostrobin, oryzastrobin, picoxystrobin, pyrazol Clostrobin, Trifloxystrobin, Carboxyne, Benalaxyl, Boscalid, Bixafen, Fenhexamide, Flutolanil, Flamethopyr, Mepronil, Metalaxyl, Mefenoxam, Ofrace, Oxadixyl, Oxycarboxyne, Penthiopyrad, Tifluzamide, Thiazinil, Dimethomorph, Flumorph, Flumethober, Fluopicolide, Carpropamide, Diclosimate, Mandipropamide, Fluazinam, Pyriphenox, Bupirimate, Cyprodinil, Phenalimol, Felimzon, Mepanipyrim, Nualimol, Pi Rimethanil, Triforine, Fenpiclonil, Fludioxonil, Algimorph, Dodemorph, Fenpropimorph, Tridemorph, Fenpropidine, Iprodione, Procymidone, Vinclozoline, Famoxadone, Phenamidon, Octylinone, Probenazole, Anilazine, Diclomazine, Pyrroquilon, Proquinazide, Tricyclazole, Captafor, Captan, Dazomet, Holpet, Phenoxanil, Quinoxyfen, Amisulbrom, Manzeb, Maneb, Metam, Methylam, Farbam, Propineb, Thiuram, Zineb, Ziram,Dietofencarb, iprofalicarb, benthiavaricarb isopropyl, propamocarb hydrochloride, thiophanate methyl, pyribencarb, Bordeaux mixture, basic copper chloride, basic copper sulfate, cupric hydroxide, 8-hydroxyquinoline copper, dozin, iminoctadine albesylate, iminoctadine acetate, guazatin, kasugamycin, streptomycin, polyoxin, oxytetracycline, validamycin A, binapacril, dinocap, dinovtone, dithianone, isoprothiolane, edifenphos, iprofenphos, fosetyl, fosetyl aluminum, pyrazophos, tolclophos methyl, chlorothalonil, dichloro Examples of fungicide components include, but are not limited to, fluanide, flusulfamide, hexachlorobenzene, phthalide, pencyclon, quintozen, cyflufenaamide, cymoxanil, dimethyrimole, etyrimole, flaraxyl, metraphenone, spiroxamine, ambam, sulfur, lime sulfur mixture, eclomazole, potassium bicarbonate, calcium bicarbonate, thiadiazine, tecrophthalam, triazine, copper nonylphenolsulfonate, hydroxyisoxazole, fluorimide, polycarbonate, metasulfocarb, EDDP, IBP, tolfenpyrad, fluopyram, isothianil, and isopyrazam. These fungicide components can be used individually or in combination of two or more.

[0077] Examples of components of insecticides, acaricides, nematicides, snailicides, and feeding inhibitors include 1,2-dichloropropane, 1,3-dichloropropene, abamectin, acephate, acequinosyl, acetamiprid, acetylone, acetophos, acetoprole, acrinasrin, acrylonitrile, afidopiropene, alanicarb, aldoxycarb, allethrin, allicin, allosamidin, alixicarb, α-cypermethrin, α-endosulfan, amidithione, amidoflumeth, aminocarb, amiton, amitraz, anabasin, aramite, Methidathion, Azadirachtin, Azamethiphos, Adinphosethyl, Adinphosmethyl, Azobenzene, Azocyclotin, Azotoate, Barium hexafluorosilicate, Valthrin, Bencrothiaz, Bendiocarb, Benfuracarb, Benoxaphos, Bensultap, Benzoximate, Benzyl benzoate, Beta-cyfluthrin, Beta-cypermethrin, Bifenazate, Bifensrin, Bifujunzhi, Binapacril, Biorethrin, Biometaethrin, Biopermethrin, Bistriflurone, Borax, Boric acid, Brofenvalerate, Bro Flanilide, Broflutrinate, Bromethrin, Bromufenvinphos, Bromoacetotamide, Bromocyclene, BromoDDT, Bromophos, Bromophosethyl, Bromopropylate, Bufencarb, Buprofezin, Butacarb, Butathiophos, Butetrin, Butocarboxime, Butonate, Butoxycarboxime, Kazusaphos, Calcium Polysulfate, Carvinphos, Campechlor, Carbanolate, Carbaryl, Carbofuran, Carbon Disulfide, Carbon Tetrachloride, Carbonyl Sulfide, Carbophenothion, Carbosulfan, Cartap, Carvacrol, Ki Nomethionate, Chloramine phosphorus, Chlorantraniliprole, Chlorbenside, Chlorbenzuron, Chlorbicycline, Chlordecone, Chlorenpentrin, Chloretoxyphos, Chlorfenapyr, Chlorphenetol, Chlorphenthone, Chlorfensulfide, Chlorfenvinphos, Chlorfluazuron, Chlormephos, Chloroform, Chloromebform, Chloromethirone, Chloropicrin, Chloroprallethrin, Chloropropylate, Chlorphoxime, Chlorprazophos, Chlorpyrifos, Chlorpyrifos-methyl, Chlorthiophos,Chromafenozide, Synerin I, Synerin II, Synerin complex, cismethrin, clenpyrin, Chloetocarb, Clofentidine, Closantel, Clothianidin, Colofonate, Copper naphthenate, Copper oleate, Copper sulfate, Coumaphos, Cumithoate, CPMC, Crotamiton, Clotoxyphos, Clufomate, Cryolite, Cyanofenphos, Cyanogen, Cyanophos, Cyanotoate, Cyantraniliprole, Cyclaniliprole, Ciclethrin, Cycloprate, Cycloprotrin, Cyenopyrafen, Cyflumetofen, Cyfluthrin, Cy Halodiamide, cyhalotrin, cyhexatin, simazole, cypermethrin, cyromazine, cythioate, dayoutong, dazomet, DBCP, DCIP, decarbofuran, deltamethrin, demefion, demefion O, demefion S, demeton, demeton methyl, demeton O, demeton O methyl, demeton S, demeton S methyl, demeton S methylsulfone, d-fanshiluquebingjuzhi, diafenthiuron, dialiphos, diamidaphos, diatomaceous earth, diazinon, dicapton, diclofenthion, diclofluani Dichlorbenzurone, dichlorvos, dichloromesothiaz, dicofol, diclesil, diclotophos, dicyclanil, dienochlor, diflovidazine, diflubenzuron, dirol, dimefluthrin, dimehox, dimethane, dimetacarb, dimethoate, dimetrin, dimethylvinphos, dimethilane, dinex, dinobton, dinocup, dinocup 4, dinocup 6, dinocton, dinopenton, dinopprop, dinosam, dinosulfone, dinotefuran, dinoterbone, diphenolane, dioxabenzophos, dioxacarb, dioxatio Diphenyl sulfone, dipimethitron, disulfiram, disulfoton, diticlophos, dithioether, d-limonene, DNOC, dofenapine, doramectin, ecdysterone, emamectin, EMPC, empenthrin, endothion, endrin, EPN, epophenonane, eprinomectin, epsilon metofluthrin, epsilon monfluorothrin, esdeparethrin, esfenvalerate, etaphos, ethiofencarb, ethione, etiprol, etoatemethyl, etoprophos, ethyl formate, ethyl DDD, ethylene dibromide,Ethylene dichloride, etofenprox, etoxazole, etrimphos, EXD, Fanfar, phenamiphos, phenazaflor, phenazaquin, fenbutatin oxide, fenchlorphos, phenetacarb, fenfluthrin, fenitrothion, phenobucarb, phenothiocarb, phenoxacrim, phenoxycarb, fenpyritrin, fenpropathrin, fenpyroximate, fenson, fensulfothion, fenthion, fenthion ethyl, fentriphanil, fenbarate, ferric phosphate, fipronil, flomethin, Flunicamide, fluacrypyrim, fluazindolin, fluazuron, flubendiamide, flubendimine, flucoflon, flucycloxlon, flucycloxlon, flucitrinate, fluenetil, fluensulfone, fluphenerim, flufenoxlon, fluphenoxystrobin, flufenprox, flufiprol, fluhexaphon, flumethrin, fluolbenside, flupyradiflon, flulalaner, fluvalinate, fluxamethamide, fonofos, formtanate, formtanate hydrochloride, formothion, formparanate Fosmethilan, fosspire, fosthiazate, fosthiethane, flamethrin, frantebfenozide, flatiocarb, fretrin, furfural, γ-cyhalothrin, γ-HCH, genit, guazatin, halfenprox, halofenozide, HCH, HEOD, heptafluthrin, heptenofos, heterophos, hexachlorophene, hexaflumuron, hexythiazox, HHDN, hydramethylnon, hydroprene, hikincarb, imiciaphos, imidacloprid, imidaclotiz, imiprothrin, indoxacarb, IPSP, isa Midophos, Isazofos, Isobenzan, Isocarbos, Isodrine, Isofenphos, Isofephosmethyl, Isolane, Isoprocarb, Isoprothiolane, Isothioate, Isoxathion, Ivermectin, Japotrin, Jasmolin I, Jasmolin II, Jiahuangchongzong, Iodophenphos, Juvenile Hormone I, Juvenile Hormone II, Juvenile Hormone III, Cadetrin, Kappabifenthrin, Kappatefluthrin, Kereban, Quinoprene, Lambdasihalotrin, Lepimectin, Leptophos, Lilynphos, Lufenuron,Lithidathion, Malathion, Malonoben, Maltodextrin, Matrin, Magidox, Mecarbam, Mecarbhon, Medimethimol, Menazone, Meperfluthrin, Mephosphoran, Mesulfen, Mesulfenphos, Metaflumizone, Metaldehyde, Metam, Methacryphos, Methidathion, Methiocarb, Metoclotophos, Methomyl, Methoprene, Methotrin, Methoxychlor, Methoxyphenozide, Methyl iodide, Methyl isothiocyanate, Methylacetophos, Methylchloroform, Methylene chloride, Metofluthrin, Metocarb, Methoxadiazone Mevinphos, Mexacalbate, Milbemectin, Milbemycin oxime, Mipahox, Milex, MNAF, Monfluorothrin, Morphothion, Moxidectin, Naphthalophos, Nared, Naphthalene, Niclosamide, Nicotin, Niflulidide, Nicomycin complex, Nitenpyram, Nichiazine, Nitrilacarb, Nornicotine, Novalon, Noviflumuron, Omethoate, Oxamyl, Oxidemetonmethyl, Oxideprophos, Oxydisulfon, Oxymatrin, Paichongding, Paradichlorobenzene, Penfluron, Pe Chlorophenol, pentometrin, permethrin, fencapton, phenothrin, fenproxide, fentoate, phorate, phosalon, phospholan, phospholanmethyl, phosglycine, phosmet, phosniclor, phosphine, phosphocarb, phostine, phoxim, phoximmethyl, pyrimetaphos, pyrimicarb, pyrimioxyphos, pyrimiphosethyl, pyrimiphosmethyl, priphenate, polythialan, potassium thiocyanate, prallethrin, precosen I, precosen II, precosen III, primidophos, proclonal, prof Enofos, Profluthrin, Promacil, Promecarb, Propafos, Propargit, Propatrin, Propetamfos, Propoxul, Protidathion, Prothiofos, Protoate, Protrefenbut, Piflubumi, Pymetrozine, Piraclofos, Pirafluprole, Pyramat, Pirazofos, Pirazothion, Pyrethmetrin, Pyrethrin I, Pyrethrin II, Pyrethrin, Pyridaben, Pyridaryl, Pyridafenthion, Pyrifluquinazon, Pyrimidifen, Pyriminostrobin, Pyrimitate, Pyriprole, Pyriproxyfen, Pyrolan,Keasia, Quinalphos, Quinalphos-methyl, Quinotion, Quinthiophos, Lafoxanide, Resmethrin, Rhodojaponin III, Rotenone, Lianya, Sabajira, Sanguinarine, Schlardan, Selamectin, Semiamitraz, Semiamitraz Chloride, Silafluofen, Silica Gel, Sodium Fluoride, Sodium Hexafluorosilicate, Sodium Chlorophenol, Sodium Tetrathiocarbonate, Sodium Thiocyanate, Sofamide, Spinetoram, Spinosad, Spiro Diclofen, spiromesifen, spirotetramate, sulcoflon, sulcoflon sodium salt, sulfiram, sulfuramide, sulfotep, sulfoxaflor, sulfoxime, sulfur, sulfuryl fluoride, sulprophos, taufluvalinate, tadimucarb, TDE, tebufenozide, tebufenpyrad, tebupyrimphos, teflubenzuron, tefluthrin, temephos, TEPP, terarethrin, terbuphos, tetrachloroethane, tetrachlorbinphos, tetradiphon, tetrameth Phosphorus, tetramethylfluthrin, tetranactin, tetraniliprole, tetrasal, tetracypermethrin, thiacloprid, thiamethoxam, thiapronil, thiocarboxim, thiocyclam, thiodicarb, thiophanox, thiofluoximate, thiometon, thionazine, thioquinox, thiosultap, thiosultap sodium salt, thioxazafen, chilpert, tolfenpyrad, tralocitrin, tralomethrin, tralopyril, transpermethrin, trialate Examples include, but are not limited to, triazamate, triazophos, trichlorfon, trichlormetaphos-3, trichloronate, triphenmorph, triphenophos, triflumezopyrim, triflumulon, trimetacarb, triprene, triptolide, valerate, bamidthion, vaniliprole, xiaochongliulin, XMC, xylenol derivatives, xylylcarb, yishijing, zetacypermethrin, zolaprophos, alpha-ecdysone, etc. These insecticides, acaricides, nematicides, snailicides, and feeding inhibitors can be used individually or in combination of two or more.

[0078] Examples of herbicides and algaecides include 2,3,6-TBA, 2,4,5-TB, 2,4-D, 2,4-DB, 2,4-DEB, 2,4-DEP, 3,4-DA, 3,4-DB, 3,4-DP, 4-CPA, 4-CPB, 4-CPP, acetochlor, acicfluorphene, acroniphene, acrolein, alidoclor, alloxidim, allyl alcohol, arolac, amethidione, ametrin, amivudine, amicarbazone, amidosulfuron, aminocyclopyrachlor, aminopyralide, amiprophos-methyl, amiprophos, amitorol, Ammonium sulfamate, anirophos, anislon, ashram, atraton, atrazine, azaphenidine, azimsulfuron, adiprothrin, barban, BCPC, beflubutamide, benazoline, bencarbazone, benfluralin, benfresate, bensulfuron, benslid, bentazon, bentranil, benzadox, benzalkonium chloride, benzfenizone, benzipram, benzobicyclon, benzofenap, benzofluol, benzoylprop, benzthiazulon, bethoxazine, bicyclopyrone, bifenox, via Lafos, Bispiribac, Borax, Bromacil, Bromobonyl, Bromobutide, Bromophenoxime, Bromoxynil, Brompyrazone, Butachlor, Butaphenacil, Butamiphos, Butenachlor, Bithiazole, Butiurone, Butraline, Butroxidime, Buturone, Butyrate, Cacodylic acid, Cafenstrol, Calcium chlorate, Calcium cyanamide, Cambenziclor, Carvaslam, Carbetamide, Carboxazole, Carfentrazone, CDEA, CEPC, Clomethoxynil, Chloramben, Chloranocril, Chlorajifop Chlorazine, chlorbromulone, chlorobufam, chloretulone, chlorfenac, chlorfenprop, chlorflurazole, chlorflurenol, chloridazone, chlorimurone, chlornidine, chloronitrofen, chloropon, chlorotolurone, chloroxurone, chloroxylin, chlorprocarb, chlorprofam, chlorsulfurone, chlortal, chlorthiamide, synidone ethyl, sinmethylline, synosulfurone, sisanilide, clasifos, cretodymium, cliodinate, clodinahop, clohop, chromazon, clomeprop,Cloprop, Cloproxidyme, Clopyralide, Chloranslam, CMA, Copper Sulfate, CPMF, CPPC, Credazine, Cresol, Cumilon, Cyanamide, Cyanatrin, Cyanazine, Cyanogen, Sibutrin, Cycloate, Cyclopyrimolate, Cyclosulfamurone, Cycloxidyme, Cycluron, Cyhalofop, Cyperquat, Ciprazine, Ciprazole, Cipromide, Dimuron, Darapon, Dazomet, Delacrol, Desmedifam, Desmethrin, Dialate, Dicanba, Diclobenyl, Diclon, Dichloralurea, Diclome Dichlorophene, dichlorprop, dichlorprop-P, diclohop, diclolam, dietamquat, dietatylethyl, diphenopenten, diphenoxuron, diphenzoquat, diflufenican, diflufenzopyr, dimeflon, dimepiperate, dimethachlor, dimethametrin, dimetenamide, dimetenamide-P, dimexano, dimidazon, dinitramine, dinophenate, dinoprop, dinosam, dinoterb, diphenamide, diproporin, dipropetrin, diquat, disulfide, dithioether, dithiopyr, diuro N, DMPA, DNOC, DSMA, EBEP, Eglinazine, Endotar, Epronaz, EPTC, Elbon, erlujixiancaoan, Esprocarb, Etachlor, Etalfluralin, Etamethulfuron, Etaprochlor, Ethidimron, Ethiolate, Ethiodin, Etofmesate, Ethoxyfen, Ethoxysulfuron, Ethinofen, Etonipromide, Etobenzanide, EXD, Phenaslam, Phenoprop, Phenoxaprop, Phenoxaprop-P, Phenoxasulfone, Fenquinotrione, Fentelacol, Fe Nthiaprop, tin, fentrazamide, fenulon, iron sulfate, flamprop, flamprop-M, flazasulfuron, floralam, fluazihop, fluazihop-P, fluazolate, flucarbazone, flucetosulfuron, fluchloralin, fluphenacet, flufenican, flufenpir, flumetulam, flumazine, flumicrolac, flumioxazine, flumipropine, fluomethron, fluorodiphen, fluoroglycofen, fluoromidine, fluoronitrophen, fluothiron, flupoxam, flupropasil,Flupropanate, Flupirsulfuron, Flulidone, Flurochloridone, Fluroxypil, Flulutamon, Fluthiaset, Fomseifen, Folamsulfuron, Hosamin, Fukaodin, Fukaomi, Funaihekaolin, Fryloxyfen, Glufosinate, Glufosinate-P, Glyphosate, Haraxifen, Halosaphen, Halosulfuron, Haloxydine, Haloxyhop, Haloxyhop-P, Herbimycin, Hexachloroacetone, Hexaflurate, Hexazinone, Huankaiwo, Huankaolin, Slaked lime, Imazametabenz, I Mazamox, Imazapix, Imazapill, Imazakine, Imazetapir, Imazosulfuron, Indanophan, Indadiflame, Iodobonyl, Iodosulfuron-methyl, Iofensulfuron, Ioxinil, Ipfencarbazone, Iprimidum, Isocarbamide, Isosyl, Isomethidine, Isonolone, Isopolinate, Isoproparin, Isoprotulone, Isouron, Isoxaben, Isoxachlorotol, Isoxaflutol, Isosapirhop, Carbutyrate, Ketospiradox, Quicaoxy, Lactofen, Rena Sil, Linuron, MAA, MAMA, MCPA, MCPA-thioethyl, MCPB, Mecoprop, Mecoprop-P, Medinoterb, Mefenacet, Mefluidide, Mesoprazine, Mesosulfuron, Mesotrione, Metam, Metamihop, Metamitron, Metazachlor, Metazosulfuron, Metoflurazone, Metabenzthiazulon, Metalpropalin, Metasol, Methiobencarb, Methiopyrisulfuron, Methiozoline, Methyluron, Metmetone, Metoprothrin, Methoxyphenone, Methyl bromide, Methyl iodide, Methyl isothiocyanate, Methyl dimuron, metobenzurone, metobromurone, metrachlor, metoslam, methoxlon, metrivudine, metosulfuron, molinate, monalide, monisouron, monochloroacetic acid, monolinuron, monosulfuron, monuron, morphamcort, MSMA, nervam, naproanilide, napropamide-M, naptalam, nebulon, nicosulfuron, nipiraclofen, nitraline, nitrofen, nitrofluorphene, norflurazone, norlon, OCH, olbencarb, o-dichlorobenzene, orthosulfamurone, oryzalin,Oxaziargyl, oxadiazone, oxapirazone, oxasulfuron, oxadiclomefone, oxyfluorphen, paraflurone, paraquat, pebrate, pelargonic acid, pendimethalin, penoxulam, pentachlorophenyl laurate, pentanochlor, pentoxazone, perfluidone, petoxamide, phenisophan, fenmedifam, fenmedifam-ethyl, phenobenzuron, picloram, picolinafene, pinoxadene, piperofos, pretilachlor, primisulfuron, procyazin, prodiamine, profluazo Profluralin, Profoxidim, Proglinadin, Prometon, Prometrin, Propachlor, Propanil, Propaxafop, Propazine, Profam, Propisochlor, Propoxycarbazone, Propyrisulfuron, Propizamide, Prosulfarin, Prosulfocarb, Prosulfuron, Proxan, Prinachlor, Pidanone, Pyraclonil, Pyraclonifen, Pyrasulfol, Pyrazolate, Pyrazosulfuron, Pyrabumbenz-isopropyl, Pyrabumbenzpropyl, Pyribenzoxime, Pyribencarb, Pyrichlor, Pyridafol, Pyridate, Pyriphthalide, Pyriminovac, Pyrimisulfan, Pyrthiovac, Pyroxasulfone, Pyroxyslam, Kinchlorac, Kinmelac, Quinoclamine, Quinonamide, Quizalohop, Quizalohop-P, Rhodetanyl, Limusulfuron, Saflufenacil, Cebutyrazine, Secubumeton, Cethoxydim, Shuangjiaancaolin, Siderone, Simazine, Symeton, Symetrin, SMA, S-Metrachloride, Sodium Chlorate, Sulcotrione, Sulfate, Sulfenthrazone, Sulfon Metsuron, sulfosulfuron, sulglycapine, swep, tabron, TCA, tebutam, tebuthiuron, tefuryltrione, tenbotrione, tepraloxidim, terbasil, terbucarb, terbuchlor, terbumetone, terbutyrazine, terbutrin, tetraflurone, tenylchlor, triaziflame, thiazopyr, thidiadimine, thidiazuron, thiencarbazone, thifensulfuron, thiobencarb, thiafenasil, thiocarbasil, thiochlorim, torpyrate, topramezone, tralcoxidim, triafamone, triateExamples include, but are not limited to, triasulfuron, tribeuron, tricamba, triclopyr, tridiphan, trietadine, trifloxysulfuron, trifludimoxazine, trifluralin, triflusulfuron, triphop, trihopsim, trihydroxytriazine, trimethuron, tripropindan, tritac, tritosulfuron, vernolate, xylacrol, etc. These herbicides and algaecides can be used individually or in combination of two or more components.

[0079] Examples of biological pesticide components include nuclear polyhedrosis virus (NPV), granulosis virus (GV), cytoplasmic polyhedrosis virus (CPV), Steinernema carpocapsae, Steinernema graseri, Monacrosporium phymatophagum, Steinernema kushidai, Pasteuria penetrans, Agrobacterium radiobacter, Bacillus subtilis, Bacillus amyloliquefaciens, and Bacillus cereus. Bacillus cereus, Bacillus pumilus, Bacillus licheniformis, Bacillus mycoides, Bacillus methylotrophycus, Bacillus simplex, Bacillus firmus, Bacillus megaterium, Bacillus coagulans, Bacillus oryzicola, Bacillus sp.), Bacillus thuringensis, Erwinia cartovora, Pseudomonas fluorescens, Pseudomonas cepacia, Pseudomonas putida, Talaromyces flavus, Trichoderma atroviride, Beauveria brongniartii, Beauveria bassiana, Paecilomyces fumosoroseus, Verticillium lecanii, Eupenicillium javanicum, Pencillium javanicum Examples include, but are not limited to, *javanicum*, *Xanthomonas campestris*, *Encarsia formosa*, *Eretmocerus eremicus*, *Eretmocerus mundus*, *Aphidoletes aphidimyza*, *Aphidoletes aphidimyza*, *Diglyphus isaea*, *Dacnusa sibirica*, *Phytoseiulus persimilis*, *Amblyseius cucumeris*, *Amblyseius californicus*, and *Oriusstrigicollis*. These biopesticides can be used individually or in combination of two or more components.

[0080] Examples of pheromone agents (insect attractants) include, but are not limited to, Brevicomin, Ceralure, Kodremon, Kyurua, Disparua, Dominicalle 1, Eugenol, Frontalin, Gosiprure, Grandis, Hexalua, Ipsdienol, Ipsenol, Japonilua, Lacyllure, Lineatin, Littleua, Lupurua, Medruua, Megatomoic acid, Methyl eugenol, Moguchun, Muscarua, Orfuralua, Orictalua, Ostramon, Rescalle, Sigurrure, Sulcatol, Trimedrua, Trunkol, α-Multistratin, etc.

[0081] Examples of pheromone agents (insect repellents) include, but are not limited to, Acrep, buttopyrronoxyl, camphor, d-camphor, carboxymide, dibutyl phthalate, diethyltoluamide, dimethyl carbonate, dimethyl phthalate, dibutyl succinate, etohexadiol, hexanoamide, picaridin, metkin-butyl, methylneodecanamide, 2-(methylthio)ethanol, oxamate, Quench, Quingzing, Rebemid, and Zengxiaoan. These pheromone agents can be used individually or in combination of two or more ingredients.

[0082] Examples of natural fungicides and insecticides include, but are not limited to, machine oil, methylphenyl acetate, α-pinene, protein hydrolysates, (Z)-1-tetradecen-1-ol, and turpentine oil. These natural fungicides and insecticides can be used individually or in combination of two or more.

[0083] (Method for controlling plant diseases) In one embodiment of the present invention, a composition for use in controlling plant diseases can be applied directly as is, or diluted with water or the like before application. The method of applying the composition for use in controlling plant diseases is not particularly limited and includes, for example, spraying it directly on plants or pathogens, spraying it on the soil, adding it to water or fertilizer added to plants or soil, or coating seeds with it. Furthermore, the amount of formulation to be applied varies depending on the target disease, target crop, application method, incidence trend, degree of damage, environmental conditions, and formulation used, so it is preferable to adjust it as appropriate.

[0084] In one embodiment of the present invention, the plants to which the control method is applied refer to plants or groups of plants such as wild plants, cultivated plants, naturally occurring plants, and cultivated plants, and also include plants produced by breeding methods such as introduction breeding, isolation breeding, hybrid breeding, hybrid vigor breeding, mutation breeding, polyploidy breeding, genetic modification (gene introduction), and marker-assisted selection. Furthermore, the plants to be treated may be the whole plant or a part of the plant. In one embodiment of the present invention, when the control method is applied to a plant, it can be applied to the plant or the area surrounding the plant. The area surrounding the plant refers to soil (soil in which seeds are sown), paddy fields, water for hydroponics, cultivation materials, etc. Furthermore, the method of application to the plant may include spraying, scattering, powdering, spraying, diffusion, immersion, drenching, injection, watering (immersion), foaming, coating, powder coating, coating, spraying, fumigation, smoke, fogging, painting, etc., and preferably includes soil mixing, drenching, seed coating, seed immersion, or foliar application. These plant application methods can be used individually or in combination of two or more methods.

[0085] In one embodiment of the present invention, when applying a composition for use in controlling plant diseases, such application can be carried out throughout the plant's growing and storage periods. Hereinafter, in one embodiment of the present invention, the control method can target part or all of the plant. Part of the plant means the leaves, stems, trunks, branches, flowers, fruiting bodies, fruits, seeds, roots, tubers, rhizomes, or a combination thereof.

[0086] In one embodiment of the present invention, a composition for use in controlling plant diseases can be used after adjusting the application rate to an amount that is effective but not toxic to plants. Here, an amount that is effective but not toxic to plants is an amount that can sufficiently control the causative agent of the plant disease without harming the plant, and this amount can vary over a relatively wide range depending on the causative agent of the plant disease to be controlled, the plant to which it is applied, the natural environment in which it is used, and the components of the composition for use in controlling plant diseases.

[0087] In one embodiment of the present invention, in the control method, all the components of the target plant disease, etc., described in the above section (Compositions for use in the control of plant diseases), can be applied individually or in combination of two or more.

[0088] The present invention will be specifically described by the following manufacturing examples, embodiments, and comparative examples, but the present invention is not limited to these examples.

[0089] 1. Efficacy Test against Plant Diseases (Obtaining various strains, culturing strains, and preparing dried powder) Various strains belonging to the genus Brevibacillus, as listed in Table 1, were obtained from NBRC. Each strain was cultured for two days in ordinary broth medium (manufactured by Eiken Chemical Co., Ltd.) and used as inoculum for subsequent culturing.

[0090]

[0091] As a pre-culture, various bacteria on ordinary broth medium were punched out along with the agar medium using a cork borer, and the pre-culture medium (maltose 0.8%, soy flour 0.8%, K ２ HPO ４ 0.01%, MgSO ４ 7H ２ O 0.02%, CaCl ２ After inoculating a 500 ml baffled Erlenmeyer flask containing 100 ml of 0.02% of the culture medium, the flask was incubated in a rotary shaker at 150 rpm at 28°C for 1 day.

[0092] Two ml of the culture obtained from the above pre-culture was further mixed with medium A (2% glucose, 0.3% yeast extract, (NH4)). ４ ) ２SO ４ 0.5%, KCl 0.4%, K ２ HPO ４ 0.02%, CaCO ３ (0.4%) 100 ml was inoculated into a 500 ml Erlenmeyer flask with a baffle, and cultured at 150 rpm, 28 °C for 4 days using a rotary shaker. Approximately 100 g of each culture obtained as described above was frozen at -80 °C, freeze-dried under reduced pressure, and pulverized. Each dry powder thus obtained was designated as Production Examples 1 to 10 (corresponding to Strain Nos. 1 to 10, respectively).

[0093] Further, separately from Production Examples 1 to 10 above, 2 ml of the culture obtained by the above preculture was further inoculated into a 500 ml Erlenmeyer flask with a baffle containing 100 ml of Medium B (dextrin 3%, glucose 0.3%, soybean meal 2%, CoCl ２ ·6H ２ O 0.01%) and cultured at 150 rpm, 28 °C for 4 days using a rotary shaker. Approximately 100 g of each culture obtained as described above was frozen at -80 °C, freeze-dried under reduced pressure, and pulverized. Each dry powder thus obtained was designated as Production Examples 11 to 20 (corresponding to Strain Nos. 1 to 10, respectively).

[0094] Furthermore, separately from Production Examples 1 to 20 above, 2 ml of the culture obtained by the above preculture was further inoculated into a 500 ml Erlenmeyer flask with a baffle containing 100 ml of Medium C (sucrose 3%, soybean meal 2%, NaCl 0.3%, CaCO ３ 0.3%) and cultured at 150 rpm, 28 °C for 4 days using a rotary shaker. Approximately 100 g of each culture obtained as described above was frozen at -80 °C, freeze-dried under reduced pressure, and pulverized. Each dry powder thus obtained was designated as Production Examples 21 to 30 (corresponding to Strain Nos. 1 to 10, respectively).

[0095] In addition, separate from the above production examples 1 to 30, 2 ml of the culture obtained by the above pre-culturing was further inoculated into a 500 ml baffled Erlenmeyer flask containing 100 ml of medium D (2% maltose, 0.2% yeast extract, 0.5% NZ Amine® (casein (enzyme hydrolysate)), 0.2% NaCl), and cultured in a rotary shaker at 150 rpm, 28°C for 4 days. Approximately 100 g of each culture obtained as described above was frozen at -80°C, then freeze-dried under reduced pressure and pulverized. The dried powders obtained in this way were designated as production examples 31 to 40 (corresponding to strains No. 1 to 10, respectively).

[0096] Next, we present test examples of compositions used in the control of plant diseases that were tested for their effectiveness.

[0097] (Test Example 1: Efficacy Test against Tomato Late Blight) Six-leaf tomatoes (variety: Regina) grown in 6 cm diameter plastic pots in a greenhouse were sprayed in sufficient quantities using a spray gun with dilutions of each of the above production examples 1 to 40, diluted 250 times with distilled water. As Comparative Example 1, a dilution of Impression Clear (registered trademark) (Bacillus amylorique faciens wettable powder, manufactured by SDS Biotech Co., Ltd.), diluted 250 times with distilled water, was used in the same manner as above. The day after spraying, a suspension of zoospores of the tomato late blight fungus (Phytophthora infestans) was spray-inoculated. The inoculated pots were kept in a humid room at 22°C for 16 hours, then left to stand in the room for 3 days, and the diseased area percentage of the third, fourth, and fifth leaves was investigated to determine the control value. The control value (%) was calculated based on the diseased area percentage of the untreated group, and the following four-stage evaluation was performed based on that control value. Control value of 80 or higher: +++, Control value of 50 or higher but less than 80: ++, Control value of 30 or higher but less than 50: +, Control value less than 30: -

[0098] (Test Example 2: Efficacy Test against Cucumber Powdery Mildew) Cucumbers in the cotyledon stage (variety: Hanshiro Setsunari, 2 seeds planted) grown in 5 cm diameter plastic pots in a greenhouse were sprayed in sufficient quantities using a spray gun with dilutions of each of the above production examples 1 to 40, diluted 250 times with distilled water. As Comparative Example 1, a dilution of the above-mentioned Impression Clear (registered trademark) diluted 250 times with distilled water was used in the same manner as above. The day after spraying, a suspension of cucumber powdery mildew fungus (Sphaerotheca fuliginea) spores was spray-inoculated. After air-drying, the plants were managed in a greenhouse for about 10 days, and the control value was determined from the diseased area percentage of the inoculated leaves. The control value (%) was calculated based on the diseased area percentage of the untreated group, and the following four levels were evaluated based on that control value. Control value of 80 or higher: +++, Control value of 50 or higher but less than 80: ++, Control value of 30 or higher but less than 50: +, Control value less than 30: -

[0099] (Test Example 3: Efficacy Test against Cucumber Gray Mold) Cucumbers at the cotyledon stage (variety: Hanshiro Setsunari, 2 seeds planted) grown in a greenhouse in 5 cm diameter plastic pots were sprayed in sufficient quantities using a spray gun with dilutions prepared by diluting each of the above production examples 1 to 40 250 times with distilled water. As Comparative Example 1, a dilution prepared by diluting the above-mentioned Impression Clear (registered trademark) 250 times with distilled water was used in the same manner as above. The day after spraying, cucumber cotyledons were cut at the hypocotyl and arranged in a plastic case under humid chamber conditions. 50 μL of a spore suspension of cucumber gray mold fungus (Botrytis cinerea) was dropped onto a PAPER DISK (antibiotic testing paper disc, thick 8 mm, manufactured by Toyo Roshi Co., Ltd.), and this was placed on the center of the cucumber cotyledons so that the dropping surface was in contact with the leaf. The diameter of lesions that appeared on cucumber leaves three days after inoculation was measured, and the control efficacy (%) was determined by comparing it with the untreated group. Based on this control efficacy, the following four-stage evaluation was performed: Control efficacy of 80 or more: +++, Control efficacy of 50 or more but less than 80: ++, Control efficacy of 30 or more but less than 50: +, Control efficacy of less than 30: -

[0100] (Test Example 4: Efficacy Test against Wheat Rust Disease) Wheat (variety: Norin No. 61, 8 plants planted) grown in a greenhouse in 5 cm diameter plastic pots until the 2-leaf stage was sprayed in sufficient quantities using a spray gun with a diluted solution of each of the above production examples 1 to 40, diluted 250 times with distilled water. As Comparative Example 1, a diluted solution of the above-mentioned Impression Clear (registered trademark) diluted 250 times with distilled water was used in the same manner as above. The day after spraying, a spore suspension of the wheat rust fungus (Puccinia recondita) was spray-inoculated. The inoculated pots were kept in a humid room at 22°C for 24 hours, then managed in a greenhouse for 10 days, and the control value was determined from the diseased area percentage of the inoculated leaves. The control value (%) was calculated based on the diseased area percentage of the untreated area, and the control value was evaluated in the following four stages. Control value of 80 or higher: +++, Control value of 50 or higher but less than 80: ++, Control value of 30 or higher but less than 50: +, Control value less than 30: -

[0101] (Test Example 5: Efficacy Test against Chinese Cabbage Black Spot Disease) Chinese cabbage (variety: Nozaki No. 2, 5 seeds planted) grown in a greenhouse in 5 cm diameter plastic pots until the 2-leaf stage was sprayed in sufficient quantities using a spray gun with dilutions of each of the above production examples 1 to 40, diluted 250 times with distilled water. As Comparative Example 1, a dilution of the above-mentioned Impression Clear (registered trademark) diluted 250 times with distilled water was used in the same manner as above. The day after spraying, a spore suspension of the Chinese cabbage black spot fungus (Alternaria brassicae) was spray-inoculated. After keeping the inoculated pots in a humid room at 22°C for 72 hours, the disease area percentage of the inoculated leaves was investigated to determine the control value. The control value (%) was calculated based on the disease area percentage of the untreated area, and the following four levels were evaluated based on that control value. Control value of 80 or higher: +++, Control value of 50 or higher but less than 80: ++, Control value of 30 or higher but less than 50: +, Control value less than 30: -

[0102] (Test Example 6: Efficacy Test against Rice Blast Disease) Rice plants (variety: Koshihikari, 12 plants planted) grown in a greenhouse in 5 cm diameter plastic pots until the 2.5-leaf stage were sprayed in sufficient quantities using a spray gun with dilutions of each of the above production examples 1 to 40, diluted 250 times with distilled water. As Comparative Example 1, a dilution of the above-mentioned Impression Clear (registered trademark) diluted 250 times with distilled water was used in the same manner as above. The day after spraying, a suspension of rice blast fungus (Pyricularia oryzae) spores was spray-inoculated. The inoculated pots were kept in a humid room at 25°C for 24 hours, then managed in a greenhouse for 7 days, and the control value was determined by investigating the number of lesions on the inoculated leaves. The control value (%) was calculated based on the number of lesions in the untreated group, and the control value was evaluated in the following four stages. Control value of 80 or higher: +++, Control value of 50 or higher but less than 80: ++, Control value of 30 or higher but less than 50: +, Control value less than 30: -

[0103] The results of Test Examples 1 to 6 are shown in Tables 2 to 5. Production Examples 3, 13, 23, and 33 are cultures of Brevibacillus brevis NBRC strain 110488 (strain 3), and production Examples 6, 16, 26, and 36 are cultures of Brevibacillus formosus NBRC strain 15716 (strain 6).

[0104]

[0105]

[0106]

[0107]

[0108] As shown in Tables 2-5, the existing commercially available bathysul amyloriquefaciens agent in Comparative Example 1 showed high disease suppression activity against two of the five diseases tested: cucumber powdery mildew and cucumber gray mold. On the other hand, production examples 3, 6, 13, 16, 23, 26, 33, and 36, which used cultures of Brevibacillus brevis NBRC 110488 or Brevibacillus formosus NBRC 15716, tended to show disease suppression activity against a wider variety of diseases compared to production examples using cultures of other strains under the same culture medium conditions. In particular, Production Example 23, using a culture of Brevibacillus brevis NBRC 110488 strain (using medium C), showed a high disease suppression effect with a control value of 80 or higher against three diseases: tomato late blight, cucumber powdery mildew, and Chinese cabbage black spot. Similarly, Production Example 26, using a culture of Brevibacillus formosus NBRC 15716 strain (using medium C), also showed a high disease suppression effect with a control value of 80 or higher against three diseases: tomato late blight, cucumber powdery mildew, and cucumber gray mold. Based on the above, it was found that Brevibacillus formosus NBRC 15716 strain and Brevibacillus brevis NBRC 110488 strain have disease-suppressing effects against a variety of diseases.

[0109] (Test Example 7: Efficacy Test 2 against Tomato Late Blight) Six-leaf tomatoes (variety: Regina) grown in 6 cm diameter plastic pots in a greenhouse were sprayed in sufficient quantities using a spray gun with dilutions of the above-mentioned Production Examples 23 and 26, diluted 250 times, 500 times, 1000 times, and 2000 times with distilled water, respectively. As Comparative Example 1, dilutions of the above-mentioned Impression Clear (registered trademark), diluted 250 times, 500 times, 1000 times, and 2000 times with distilled water, respectively, were used in the same manner as above. The day after spraying, a suspension of zoospores of the tomato late blight fungus (Phytophthora infestans) was spray-inoculated. The inoculated pots were kept in a humid room at 22°C for 16 hours, then left to stand in the room for 3 days, and the diseased area percentage of the third, fourth, and fifth leaves was investigated to determine the control value. The control value (%) was calculated based on the diseased area percentage of the untreated group. The results are shown in Table 6.

[0110]

[0111] As shown in Table 6, the commercially available bachysul amyloriquefaciens agent used as Comparative Example 1 had a control efficacy of 12.1 even after a 250-fold dilution. On the other hand, in Production Examples 23 and 26, the control efficacy was 80 or higher even after a 1000-fold dilution, and both Production Examples 23 and 26 showed remarkably high control efficacy against tomato blight.

[0112] (Test Example 8: Efficacy Test against Grape Downy Mildew) Leaf discs were cut from the true leaves of grapes (variety: Delaware) cultivated in the field using a 3 cm diameter cork borer. A sufficient amount of diluted solutions of Production Example 23 and Production Example 26, each diluted 1000 times with distilled water, was sprayed onto the underside of the prepared leaf discs using a spray gun. As Comparative Example 1, a diluted solution of Impression Clear (registered trademark) mentioned above, diluted 500 times with distilled water, was tested in the same manner as above. Furthermore, as Comparative Example 2, a diluted solution of Arietty (registered trademark) wettable powder (fosetil wettable powder) (manufactured by Bayer CropScience Co., Ltd.), diluted 800 times with distilled water, was tested in the same manner as above. After the spray solution was air-dried, a suspension of zoosporangia of the grape downy mildew fungus (Plasmopara viticola) was spray-inoculated. The inoculated leaf discs were collected in plastic cases under humid conditions and left to stand for one week. The disease area percentage of lesions that appeared on the leaf discs was then investigated to determine the control efficacy. The control efficacy (%) was calculated based on the disease area percentage of the untreated group. The results are shown in Table 7.

[0113]

[0114] As shown in Table 7, the commercially available bachysul amyloriquefaciens agent used as Comparative Example 1 had a control efficacy of 61.8 even after a 500-fold dilution. On the other hand, manufacturing examples 23 and 26 showed a control efficacy of 80 or higher even after a 1000-fold dilution, demonstrating extremely high control efficacy against grape downy mildew. Comparative Example 2 also showed high control efficacy with a control efficacy of 97.6, but fosetyl wettable powder like that used in Comparative Example 2 is a chemical agent, and therefore has limitations on the number of applications due to the risk of resistance development and impact on humans, animals, and the environment. In contrast to Comparative Example 2, manufacturing examples 23 and 26 have no restrictions on the number of applications and can be used until just before harvest, thus demonstrating high utility.

[0115] (Test Example 9: Efficacy Test against Cucumber Brown Spot Disease) Three-leaf cucumbers (variety: High Green 21) grown in 6 cm diameter plastic pots in a greenhouse were sprayed in sufficient quantities using a spray gun with dilutions of the above-mentioned Production Examples 23 and 26, diluted 250 times, 500 times, and 1000 times, respectively, with distilled water. As Comparative Example 1, dilutions of the above-mentioned Impression Clear (registered trademark) diluted 250 times, 500 times, and 1000 times, respectively, with distilled water were tested in the same manner as above. As Comparative Example 3, a dilution of Daconil (registered trademark) 1000 (tetrachloroin sophthalonitrile (TPN) wettable powder, manufactured by SDS Biotech Co., Ltd.) diluted 1000 times with distilled water was tested in the same manner as above. The day after spraying, a spore suspension of the cucumber brown spot fungus (Corynespora cassiicola) was spray-inoculated. The inoculated pots were kept in a 25°C humid chamber for 24 hours, then managed in a greenhouse for one week. The diseased area percentage of the second and third leaves was investigated to determine the control efficacy. The control efficacy (%) was calculated based on the diseased area percentage of the untreated group. The results are shown in Table 8.

[0116]

[0117] As shown in Table 8, the existing commercially available batisl amyloriquefaciens agent in Comparative Example 1 had a control efficacy of 32.8 even after a 250-fold dilution. On the other hand, production examples 23 and 26 showed extremely high control efficacy against cucumber brown spot disease, with a control efficacy of 88 or higher after a 250-fold dilution and a control efficacy of 40 or higher after a 1000-fold dilution. Comparative Example 3 also showed high control efficacy, but TPN wettable powders like Comparative Example 3 are chemical agents, and there are limitations on the number of applications due to the risk of resistance development and impacts on humans, animals, and the environment. In contrast to the above TPN wettable powders, production examples 23 and 26 have no restrictions on the number of applications and can be used until just before harvest, thus demonstrating high utility.

[0118] (Test Example 10: Efficacy Test against Cucumber Bacterial Spot Disease) Three-leaf cucumbers (variety: Hanshiro Setsunari) grown in 6 cm diameter plastic pots in a greenhouse were sprayed in sufficient quantities onto the undersides of the leaves of three-leaf cucumbers using a spray gun. Dilutions of Production Examples 23 and 26, diluted 500 times, 1000 times, and 2000 times respectively with distilled water were used as Comparative Example 1, and dilutions of Impression Clear (registered trademark) diluted 500 times, 1000 times, and 2000 times respectively with distilled water were used as Comparative Example 4, and a dilution of MycoShield (registered trademark) (oxytetracycline wettable powder) (manufactured by Nippon Soda Co., Ltd.) diluted 1000 times with distilled water were used as Comparative Example 4. The following day after spraying, a suspension of the cucumber bacterial spot disease fungus (Pseudomonas syringae) was spray-inoculated. The inoculated pots were kept in a humid chamber at 25°C for 48 hours, then managed in a greenhouse for one week. The diseased area percentage of the first and second leaves was investigated to determine the control efficacy. The control efficacy (%) was calculated based on the diseased area percentage of the untreated group. The results are shown in Table 9.

[0119]

[0120] As shown in Table 9, the existing commercially available batisl amyloriquefaciens agent in Comparative Example 1 had a control efficacy of 25.9 even after a 500-fold dilution. On the other hand, in Production Examples 23 and 26, the control efficacy was 66 or higher after a 500-fold dilution and 61 or higher after a 2000-fold dilution, indicating that treatment with the diluted solutions of Production Examples 23 and 26 provided extremely high control efficacy against cucumber bacterial spot disease. Comparative Example 4 also showed high control efficacy, but oxytetracycline wettable powder like that in Comparative Example 4 is an agricultural antibiotic and has limitations on the number of applications due to the risk of resistance development and impacts on humans, animals, and the environment. On the other hand, unlike the above oxytetracycline wettable powder, Production Examples 23 and 26 have no restrictions on the number of applications and can be used until just before harvest, thus demonstrating high utility.

[0121] (Test Example 11: Efficacy Test against Chinese Cabbage Soft Rot) Two-leaf Chinese cabbage (variety: Nozaki No. 2) grown in 5 cm diameter plastic pots in a greenhouse was sprayed in sufficient quantities three times at 7-day intervals using a spray gun with dilutions of Production Examples 23 and 26, each diluted 500 times with distilled water. As Comparative Example 5, a dilution of BioKeeper (registered trademark) (non-pathogenic Ervinia carotovora wettable powder) (manufactured by Nissan Chemical Corporation) diluted 500 times with distilled water was used in the same manner as above. The day after the final spraying, a suspension of the Chinese cabbage soft rot fungus (Pectobacterium carotovorum) was spray-inoculated. The inoculated pots were kept in a humid chamber at 25°C for 48 hours, then managed in a greenhouse for 4 days, and the disease area percentage of the entire plant was investigated to determine the control value. The control value (%) was calculated based on the disease area percentage of the untreated group. The results are shown in Table 10.

[0122]

[0123] As shown in Table 10, the existing commercially available microbial formulation in Comparative Example 5 only achieved a control value of 22. On the other hand, production examples 23 and 26 achieved a control value of 44 or higher, and treatment with diluted solutions of production examples 23 and 26 yielded extremely high control efficacy against Chinese cabbage soft rot.

[0124] (Test Example 12: Efficacy Test against Cucumber Downy Mildew) Cucumbers (variety: Hanpaku Setsunari) at the 2.5-leaf stage, grown in 6 cm diameter plastic pots in a greenhouse, were sprayed in sufficient quantities using a spray gun onto the undersides of their leaves. Dilutions of the above-mentioned production examples 23 and 26, diluted 250 times, 500 times, and 1000 times respectively with distilled water, were used in dilutions of these solutions. As Comparative Example 6, a dilution of Ranman® Flowable (cyazofamide wettable powder, manufactured by Ishihara Bioscience Co., Ltd.), diluted 1000 times with distilled water, was used in the same manner as above. The day after spraying, a suspension of cucumber downy mildew (Pseudoperonospora cubensis) zoosporangia was spray-inoculated onto the undersides of the leaves. The inoculated pots were kept in a humid chamber at 22°C for 18 hours, then left to stand indoors for 5 days. The diseased area percentage of the first and second leaves was investigated, and the control value was determined. The control value (%) was calculated based on the diseased area percentage of the untreated group. The results are shown in Table 11.

[0125]

[0126] As shown in Table 11, production examples 23 and 26 showed a control efficacy of 88 or higher when treated with a 500-fold diluted solution, demonstrating extremely high control efficacy against cucumber downy mildew. Comparative example 6 also showed a high control efficacy, but cyazofamide wettable powders like Comparative example 6 are chemical agents, and their use is limited due to the risk of developing resistant strains and their impact on humans, animals, and the environment. On the other hand, unlike the cyazofamide wettable powders mentioned above, production examples 23 and 26 have no restrictions on the number of applications and can be used until just before harvest, thus demonstrating high utility.

[0127] (Test Example 13: Efficacy Test against Onion Soft Rot (Field Test)) Open-field cultivated onions (Variety: Neo Earth, Test plot: 1.6m ２ The test was conducted using a 3-stage system (60 plants per stage, 3 consecutive stages). Dilutions of each of the above production examples 23 and 26, diluted 500 times with distilled water, were sprayed three times at 7-day intervals using a backpack-type power sprayer, and the diseased plant rate in each stage was calculated 9 days after the final treatment. As comparative example 7, a diluted solution of Kocide® 3000 wettable powder (copper hydroxide, manufactured by Kumiai Chemical Industry Co., Ltd.), diluted 1000 times with distilled water, was tested in the same manner as above. The control value (%) was calculated based on the diseased plant rate in the untreated stage. The results are shown in Table 12.

[0128]

[0129] As shown in Table 12, production examples 23 and 26 achieved a control value of 41.2 or higher, demonstrating a high control effect. Comparative Example 7 also showed a high control value, but chemical agents using inorganic copper as an active ingredient, such as Comparative Example 7, have limitations on the number of applications due to their impact on humans, animals, and the environment. On the other hand, unlike the copper wettable powders mentioned above, production examples 23 and 26 have no restrictions on the number of applications and can be used until just before harvest, thus demonstrating high utility.

[0130] (Test Example 14: Efficacy Test against Cucumber Powdery Mildew (Field Test)) Greenhouse-grown cucumbers (Variety: Yuumi, Test plot: 5m ２The test was conducted using a 3-unit system with 120 leaves per unit. Dilutions of each of the above production examples 23 and 26, diluted 1000 times with distilled water, were sprayed twice at 7-day intervals using a backpack-type power sprayer. Seven days after the final treatment, 120 leaves per unit were evaluated based on the disease index below, and the disease severity of each unit was calculated using the following formula. As comparative example 3, a dilution of the above-mentioned Daconil® 1000 diluted 1000 times with distilled water was used in the same manner as above.

[0131] Disease index 0: No disease observed. Disease index 1: Lesion area covers less than 5% of the leaf area. Disease index 2: Lesion area covers 5% or more but less than 25% of the leaf area. Disease index 3: Lesion area covers 25% or more but less than 50% of the leaf area. Disease index 4: Lesion area covers 50% or more of the leaf area.

[0132] Disease severity = ((1 × n1 + 2 × n2 + 3 × n3 + 4 × n4) / (4 × total number of leaves surveyed × 100)) *n1 to n4 are the number of leaves corresponding to disease severity index 1 to 4, respectively.

[0133] The control efficacy (%) was calculated based on the disease incidence in the untreated group. The results are shown in Table 13.

[0134]

[0135] As shown in Table 13, production examples 23 and 26 achieved a control value of 42.7 or higher, demonstrating a high control effect. Comparative Example 3 also showed a high control value, but TPN wettable powder like Comparative Example 3 is a chemical agent and has limitations on the number of applications due to its impact on humans, animals, and the environment. On the other hand, unlike the above TPN wettable powder, production examples 23 and 26 have no restrictions on the number of applications and can be used until just before harvest, thus demonstrating high utility.

[0136] 2. Examination of Formulations Examples of formulations are shown below. Note that "parts" refers to parts by mass.

[0137] (Formulation Example 1) A wettable powder was obtained by mixing and grinding 80 parts of the dried powder obtained by Production Example 23 and 20 parts of white carbon.

[0138] (Formulation Example 2) A wettable powder was obtained by mixing and grinding 80 parts of the dried powder obtained in Production Example 26 and 20 parts of white carbon.

[0139] (Formulation Example 3) 30 parts of the dried powder obtained in Production Example 23, 68 parts of white carbon, and 2 parts of polyoxyethylene sorbitol ester were mixed and ground, and granulated to obtain a granular wettable powder.

[0140] (Formulation Example 4) 30 parts of the dried powder obtained in Production Example 26, 68 parts of white carbon, and 2 parts of polyoxyethylene sorbitol ester were mixed and ground, and granulated to obtain a granular wettable powder.

[0141] (Formulation Example 5) In the above "1. Efficacy Test against Plant Diseases" (Obtaining various strains, culturing strains, and preparing dried powder), strain No. 3 (Brevibacillus brevis NBRC 110488) was used, and pre-culturing was performed using the same procedure and method. Then, 2 ml of the culture obtained from the above pre-culturing was further cultured in medium C (sucrose 3%, soybean meal 2%, NaCl 0.3%, CaCo ３ After inoculating a 500 ml baffled Erlenmeyer flask containing 100 ml of 0.3% of the culture medium, the flask was cultured in a rotary shaker at 150 rpm and 28°C for 4 days to obtain a culture medium. 95 parts of the culture medium obtained in this way and 5 parts of calcium chloride were mixed to obtain a liquid formulation.

[0142] (Formulation Example 6) In the above "1. Efficacy Test against Plant Diseases" (Obtaining various strains, culturing strains, and preparing dried powder), strain No. 6 (Brevibacillus formosus NBRC 15716) was used, and pre-culturing was performed using the same procedure and method. Then, 2 ml of the culture obtained from the above pre-culturing was further cultured in medium C (sucrose 3%, soybean meal 2%, NaCl 0.3%, CaCo ３ After inoculating a 500 ml baffled Erlenmeyer flask containing 100 ml of 0.3% of the culture medium, the flask was cultured in a rotary shaker at 150 rpm and 28°C for 4 days to obtain a culture medium. 95 parts of the culture medium obtained in this way and 5 parts of calcium chloride were mixed to obtain a liquid formulation.

[0143] (Test Example 15: Efficacy Test against Tomato Late Blight 3: Efficacy of Wettable Powder) Production Example 23 or 26 (dried powder) was diluted with distilled water to 500 times, 1000 times, and 2000 times, respectively. Also, the wettable powders of Formulation Example 1 or 2 were diluted with distilled water to 400 times, 800 times, and 1600 times, respectively. The dilutions obtained in this way were adjusted so that the dilution ratios of the original dried powder were 500 times, 1000 times, and 2000 times, respectively, as in Production Examples 23 and 26. Six-leaf tomatoes (variety: Regina) grown in 6 cm diameter plastic pots in a greenhouse were sprayed in sufficient quantities using a spray gun with the dilutions of Production Examples 23 and 26 and Formulation Examples 1 and 2. As Comparative Example 6, a dilution of the above-mentioned Lanman® Flowable diluted 2000 times with distilled water was used in the same manner as above. The day after spraying, a suspension of zoospores of the tomato blight fungus (Phytophthora infestans) was spray-inoculated. The inoculated pots were kept in a humid chamber at 22°C for 16 hours, then left to stand indoors for 3 days. The diseased area percentage of the third, fourth, and fifth leaves was investigated, and the control efficacy was determined. The control efficacy (%) was calculated based on the diseased area percentage of the untreated group. The results are shown in Table 14.

[0144]

[0145] As shown in Table 14, formulations 1 and 2, which are wettable powders of cultures of Brevibacillus brevis NBRC 110488 and Brevibacillus formosus NBRC 15716 strains (production examples 23 and 26), both showed a control value of 55 or higher against tomato blight when treated with a 1000-fold dilution, and 95 or higher when treated with a 500-fold dilution, demonstrating a high control effect.

[0146] (Test Example 16: Efficacy Test 4 against Tomato Late Blight - Efficacy of Granular Wettable Powder) The above production example 23 or 26 (dried powder) was diluted with distilled water to 500 times, 1000 times, and 2000 times, respectively. The granular wettable powder of formulation example 3 or 4 was diluted with distilled water to 150 times, 300 times, and 600 times, respectively. The dilutions obtained in this way were adjusted so that the dilution ratios of the original dried powder were 500 times, 1000 times, and 2000 times, respectively, as in production examples 23 and 26. Six-leaf tomatoes (variety: Regina) grown in 6 cm diameter plastic pots in a greenhouse were sprayed in sufficient quantities using a spray gun with the dilutions from production examples 23 and 26 and formulation examples 3 and 4. As comparative example 6, a dilution of the above-mentioned Lanman® Flowable diluted 2000 times with distilled water was used in the same manner as above. The day after spraying, a suspension of zoospores of the tomato blight fungus (Phytophthora infestans) was spray-inoculated. The inoculated pots were kept in a humid chamber at 22°C for 16 hours, then left to stand indoors for 3 days. The diseased area percentage of the third, fourth, and fifth leaves was investigated, and the control efficacy was determined. The control efficacy (%) was calculated based on the diseased area percentage of the untreated group. The results are shown in Table 15.

[0147]

[0148] As shown in Table 15, the cultures of Brevibacillus brevis NBRC 110488 and Brevibacillus formosus NBRC 15716 strains (production examples 23 and 26) and their respective granular wettable formulations, formulation examples 3 and 4, all demonstrated high control efficacy against tomato blight, with a control value of 80 or higher when treated with a 1000-fold dilution and a control value of 63 or higher when treated with a 2000-fold dilution.

[0149] (Test Example 17: Efficacy Test 5 against Tomato Late Blight - Efficacy of Liquid Formulation) The above production example 23 or 26 (dried powder) was diluted with distilled water to 500 times, 1000 times, and 2000 times, respectively. In addition, the liquid formulation of Formulation Example 5 or 6 was diluted with distilled water to 20 times, 40 times, and 80 times, respectively. The dilutions obtained in this way were adjusted to correspond to dilution ratios of 500 times, 1000 times, and 2000 times, respectively, for the dried powder, similar to production examples 23 and 26. Dilutions of production examples 23 and 26 and formulation examples 5 and 6 were sprayed in sufficient quantities using a spray gun onto 6-leaf stage tomatoes (variety: Regina) grown in 6 cm diameter plastic pots in a greenhouse. As Comparative Example 6, a dilution of the above-mentioned Lanman® Flowable diluted 2000 times with distilled water was used in the same manner as above. The day after spraying, a suspension of zoospores of the tomato blight fungus (Phytophthora infestans) was spray-inoculated. The inoculated pots were kept in a humid chamber at 22°C for 16 hours, then left to stand indoors for 3 days. The diseased area percentage of the third, fourth, and fifth leaves was investigated, and the control efficacy was determined. The control efficacy (%) was calculated based on the diseased area percentage of the untreated group. The results are shown in Table 16.

[0150]

[0151] As shown in Table 16, the cultures of Brevibacillus brevis NBRC 110488 and Brevibacillus formosus NBRC 15716 strains (production examples 23 and 26), and their respective liquid formulations, formulation examples 5 and 6, all demonstrated a control titer of 93 or higher against tomato blight when treated with a 1000-fold diluted solution, demonstrating a high level of control efficacy.

Claims

1. A composition for use in controlling plant diseases, containing as an active ingredient at least one selected from the group consisting of the cells or cultures thereof of Brevibacillus formosus strain NBRC 15716 or its mutant strain, and Brevibacillus brevis strain NBRC 110488 or its mutant strain, or its culture.

2. The composition according to claim 1, wherein the plant disease is a plant disease caused by bacteria, basidiomycetes, ascomycetes, imperfect fungi, oomycetes, or zygomycetes.

3. The composition according to claim 2, wherein the plant disease is a plant disease caused by oomycetes.

4. The composition according to any one of claims 1 to 3, comprising the cell or culture of Brevibacillus formosus strain NBRC 15716 or a mutant thereof as an active ingredient, wherein the plant disease is a plant disease caused by oomycetes.

5. The composition according to any one of claims 1 to 3, comprising the cell or culture of Brevibacillus brevis strain NBRC 110488 or a mutant thereof as an active ingredient, wherein the plant disease is a plant disease caused by oomycetes.

6. A method for controlling plant diseases, comprising applying a composition according to any one of claims 1 to 5 to a target plant.

Images