A system for 3D cultivation of plant cells and methods of use

The packed bed bioreactor system addresses inefficiencies in plant cell cultivation by enhancing medium contact and using specific precursors and elicitation, resulting in substantial biomass and metabolite yield improvements.

AU2024286662B2Pending Publication Date: 2026-07-09PLURI BIOTECH LTD
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Patent Information

Authority / Receiving Office
AU · AU
Patent Type
Applications
Current Assignee / Owner
PLURI BIOTECH LTD
Filing Date
2024-03-18
Publication Date
2026-07-09

AI Technical Summary

Technical Problem

Existing bioreactor systems for plant cell cultivation face inefficiencies in biomass accumulation and secondary metabolite production, particularly in suspension cultures, leading to suboptimal yields and product profiles.

Method used

A packed bed bioreactor system with a rotating cylinder and cell-lift impeller mechanism enhances contact between growth medium and plant cells, allowing for improved biomass accumulation and metabolite production through controlled stirring and perfusion, utilizing specific precursors and elicitation methods to stimulate secondary metabolite synthesis.

Benefits of technology

The system achieves a significant increase in biomass yield and metabolite production, such as cannabinoids, carnosic acid, and vanillin, with up to 1000-fold improvements in cannabinoid concentration and 3.76-fold biomass accumulation compared to conventional suspension cultures.

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Abstract

This invention is directed to a method for three-dimensional cultivation of proliferating plant cells in a packed bed bioreactor, the bioreactor comprising at least one packed bed chamber configured to confine said proliferating plant cells within it such that the proliferating plant cells become a three-dimensional stationary biomass phase; and At least one container comprising a fluid media, the fluid media is configured to flow through said proliferating plant cells stationary biomass phase, wherein, the flow of the fluid media through the proliferating plant cells stationary biomass phase allows transfer of compounds from the fluid media into the cells and vice versa in low shear forces within the at least one packed bed chamber thereby imitating natural growth environment of the proliferating plant cells within the 3D bioreactor.
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