Plant regulatory elements and uses thereof

Abstract

The present invention provides DNA molecules and constructs comprising nucleotide sequences thereof useful for modulating gene expression in plants and plant cells. Also provided are transgenic plants, plant cells, plant parts, seeds, and commodities comprising DNA molecules operably linked to a heterologous transcribable polynucleotide, and methods of use thereof.

Description

[0001] This application is a divisional application of patent application No. 201280031326.X, filed on May 11, 2012, entitled "Plant Regulatory Elements and Their Applications".

[0002] Citation of relevant applications

[0003] This application claims the benefit of U.S. Provisional Application No. 61 / 485,876, filed May 13, 2011, which is incorporated herein by reference in its entirety.

[0004] Introduction of sequence lists

[0005] The sequence list contained in a file named "MONS304WO.txt" is hereby submitted electronically and incorporated herein by reference; the sequence list is 463 kilobytes in size (according to Microsoft). (Measurement) and created on May 9, 2012. Invention Field

[0006] This invention relates to the fields of plant molecular biology and plant genetic engineering, as well as DNA molecules that can be used to regulate gene expression in plants. background

[0007] Regulatory elements are genetic elements that regulate gene activity by modulating the transcription of operatively linked, transcribed polynucleotide molecules. These elements include promoters, leader sequences, introns, and 3′ untranslated regions, and are used in plant molecular biology and plant genetic engineering. Invention Overview

[0008] This invention provides novel gene regulatory elements, such as promoters, leader sequences, and introns, derived from melon (Cucumis melo, a plant variety commonly known as cantaloupe) for use in plants. This invention also provides DNA constructs containing said regulatory elements, transgenic plant cells, plants, and seeds. The sequences can be operatively linked to transcribed polynucleotide molecules, which may be heterologous to the regulatory sequences provided herein. This invention also provides methods for preparing and using said regulatory elements, DNA constructs containing said regulatory elements, and transgenic plant cells, plants, and seeds containing regulatory elements operatively linked to transcribed polynucleotide molecules.

[0009] Therefore, in one aspect, the present invention provides a DNA molecule, such as a set of regulatory expression elements for transcription, or a promoter, or a leader sequence, or an intron, comprising a polynucleotide sequence selected from the group consisting of: a) a sequence having at least 85% sequence identity with any one of SEQ ID NO: 1-199, 211, and 212; b) a sequence comprising any one of SEQ ID NO: 1-199, 211, and 212; and c) a fragment of any one of SEQ ID NO: 1-199, 211, and 212 exhibiting gene regulatory activity, wherein the DNA molecule is operatively linked to a heterologous transcribed polynucleotide molecule. In a specific embodiment, the set of regulatory expression elements for transcription, or a promoter, or a leader sequence, or an intron is at least 90%, at least 95%, at least 98%, or at least 99% identical to any one of SEQ ID NO: 1-199, 211, and 212. In a particular embodiment, the heterologous transcribed polynucleotide molecule contains genes for agronomic purposes, genes that can provide herbicide resistance in plants, or genes that can provide plant resistance to pests.

[0010] The present invention also provides transgenic plant cells containing a set of transcriptional regulatory expression elements, or a promoter, or a leader sequence, or an intron, wherein the DNA molecule comprises a polynucleotide sequence selected from the group consisting of: a) a sequence having at least 85% sequence identity with any one of SEQ ID NO: 1-199, 211, and 212; b) a sequence comprising any one of SEQ ID NO: 1-199, 211, and 212; and c) a fragment exhibiting gene regulatory activity of any one of SEQ ID NO: 1-199, 211, and 212, wherein the DNA molecule is operatively linked to a heterologous transcribed polynucleotide molecule. Furthermore, the set of transcriptional regulatory expression elements, or the promoter, or the leader sequence, or the intron regulates gene expression. The transgenic plant cells may be monocotyledonous or dicotyledonous plant cells.

[0011] The present invention further provides a transgenic plant or a portion thereof containing a set of transcriptional regulatory expression elements, or a promoter, or a leader sequence, or an intron, wherein the DNA molecule comprises a polynucleotide sequence selected from the group consisting of: a) a sequence having at least 85% sequence identity with any one of SEQ ID NO: 1-199, 211, and 212; b) a sequence comprising any one of SEQ ID NO: 1-199, 211, and 212; and c) a fragment exhibiting gene regulatory activity of any one of SEQ ID NO: 1-199, 211, and 212, wherein the DNA molecule is operatively linked to a heterologous transcribed polynucleotide molecule. In a specific embodiment, the transgenic plant may be a progeny plant of any generation containing the set of transcriptional regulatory expression elements, or a promoter, or a leader sequence, or an intron.

[0012] Furthermore, transgenic seeds containing a set of regulatory expression elements, promoters, leader sequences, or introns of DNA molecules such as transcriptional molecules are provided, said DNA molecules comprising polynucleotide sequences selected from the group consisting of: a) sequences having at least 85% sequence identity with any one of SEQ ID NO: 1-199, 211, and 212; b) sequences comprising any one of SEQ ID NO: 1-199, 211, and 212; and c) fragments exhibiting gene regulatory activity of any one of SEQ ID NO: 1-199, 211, and 212, wherein said DNA molecules are operatively linked to heterologous transcribed polynucleotide molecules.

[0013] In another aspect, the present invention provides a method for producing a commercial product from a transgenic plant, a transgenic plant portion, or a transgenic seed containing a set of regulatory expression elements, or a promoter, or a leader sequence, or an intron, such as a DNA molecule, the DNA molecule comprising a polynucleotide sequence selected from the group consisting of: a) a sequence having at least 85% sequence identity with any one of SEQ ID NO: 1-199, 211, and 212; b) a sequence comprising any one of SEQ ID NO: 1-199, 211, and 212; and c) a fragment of any one of SEQ ID NO: 1-199, 211, and 212 exhibiting gene regulatory activity, wherein the DNA molecule is operatively linked to a heterologous, transcribed polynucleotide molecule. In one embodiment, the commercial product is a protein concentrate, a protein isolate, a cereal, starch, a seed, a millet, flour, biomass, or seed oil.

[0014] In another aspect, the present invention provides goods comprising a set of regulatory expression elements, or promoters, or leader sequences, or introns of a DNA molecule such as a transcriptional molecule, said DNA molecule comprising a polynucleotide sequence selected from the group consisting of: a) a sequence having at least 85% sequence identity with any one of SEQ ID NO: 1-199, 211, and 212; b) a sequence comprising any one of SEQ ID NO: 1-199, 211, and 212; and c) a fragment of any one of SEQ ID NO: 1-199, 211, and 212 exhibiting gene regulatory activity, wherein said DNA molecule is operatively linked to a heterologous transcribed polynucleotide molecule.

[0015] In another aspect, the present invention provides a method for expressing a transcribed polynucleotide molecule in a transgenic plant using a DNA molecule such as a set of regulatory expression elements for transcription, or a promoter, or a leader sequence, or an intron, said DNA molecule having a DNA sequence that is at least 85% identical to any one of SEQ ID NO: 1-199, 211, and 212, or contains any one of SEQ ID NO: 1-199, 211, and 212 or consists of fragments of any one of SEQ ID NO: 1-199, 211, and 212; and cultivating said transgenic plant.

[0016] Brief description of the sequence

[0017] SEQ ID NO: 1, 5, 7, 9, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38 ,39,40,41,42,43,44,45,46,47,48,49,50,51,52,53,54,55,56,57,58,59,60,61,62,63,64,65,66,67,68,6 9, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 159, 162, 167, 168, 172, 175, 176, 177, 178, 181, 182, 183, 184, 185, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 211, and 212 are regulatory expression elements or EXP sequences of the genus *Calamus*, consisting of any of the following elements: promoter elements that operatively link a leader sequence element; promoter elements that operatively link a leader sequence element and an intron element; or promoter elements that operatively link a leader sequence element, operatively link an intron element, or operatively link a leader sequence element.

[0018] SEQ ID NO: 2, 6, 8, 10, 12, 163 and 169 are promoter elements.

[0019] SEQ ID NO: 3, 164, 166 and 170 are leader sequences.

[0020] SEQ ID NO: 4, 165 and 171 are intron sequences.

[0021] SEQ ID NO: 157, 160, 173, 179 and 186 are sequences in which the promoter is operatively connected to the leader sequence element.

[0022] SEQ ID NO: 158, 161, 174, 180 and 187 are sequences in which introns are operatively connected to leader sequence elements. Brief description of the attached figures

[0023] Figures 1a-1f A comparison of promoter variant fragments corresponding to promoter elements isolated from melon is depicted. In particular, Figures 1a-1f The 2068 bp promoter sequence P-CUCme.Ubq1-1:1:15 (SEQ ID NO: 2) found in the transcriptional regulatory expression element group EXP-CUCme.Ubq1:1:1 (SEQ ID NO: 1) is compared with the promoter sequence P-CUCme.Ubq1-1:1:15 derived via the deletion of the 5′ promoter. Deletion, for example, of the 5′ end of P-CUCme.Ubq1-1:1:15, produces the following promoters: the 1459bp promoter P-CUCme.Ubq1-1:1:16 (SEQ ID NO: 6) found in EXP-CUCme.Ubq1:1:2 (SEQ ID NO: 5); the 964bp sequence P-CUCme.Ubq1-1:1:17 (SEQ ID NO: 8) contained in EXP-CUCme.Ubq1:1:3 (SEQ ID NO: 7); the 479bp sequence P-CUCme.Ubq1-1:1:18 (SEQ ID NO: 10) contained in EXP-CUCme.Ubq1:1:4 (SEQ ID NO: 9); and the sequence P-CUCme.Ubq1-1:1:18 (SEQ ID NO: 10) contained in EXP-CUCme.Ubq1:1:5 (SEQ ID NO: 6). The 173bp sequence P-CUCme.Ubq1-1:1:19 (SEQ ID NO: 12) is located in NO: 11. Invention Details

[0025] The invention disclosed herein provides polynucleotide molecules with favorable gene regulatory activity obtained from melon. The design, construction, and use of these polynucleotide molecules are described. The nucleotide sequences of these polynucleotide molecules are provided in SEQ ID NOs: 1-199, 211, and 212. For example, these polynucleotide molecules can influence the expression of transcribed polynucleotide molecules operably linked in plant tissues, thus selectively regulating gene expression or the activity of gene-encoding products in transgenic plants. The invention also provides methods for modifying, preparing, and using said polynucleotide molecules. The invention further provides compositions containing said promoters and / or other disclosed nucleotide sequences, transformation of host cells, transgenic plants, and seeds, as well as methods for preparing and using them.

[0026] The following definitions and methods are provided to better define the invention and to guide those skilled in the art in its implementation. Unless otherwise stated, these terms are to be understood according to their conventional usage by those skilled in the art.

[0027] DNA molecules

[0028] As used herein, the term "DNA" or "DNA molecule" refers to a double-stranded DNA molecule of genomic or synthetic origin, that is, a polymer or polynucleotide molecule of deoxynucleotide bases, read from the 5′ (upstream) end to the 3′ (downstream) end. As used herein, the term "DNA sequence" refers to the nucleotide sequence of a DNA molecule.

[0029] As used herein, the term "separated DNA molecule" refers to a DNA molecule that is at least partially separated from other molecules that are normally associated with it in their natural or native state. In one embodiment, the term "separated" refers to a DNA molecule that is at least partially separated from some nucleic acids that are normally attached to the sides of the DNA molecule in their natural or native state. Thus, DNA molecules fused to regulatory or coding sequences that are normally unrelated to them (e.g., as a result of recombination techniques) are considered separated herein. When integrated into the chromosome of a host cell or present in a nucleic acid solution with other DNA molecules, such molecules are considered separated because they are not in their native state.

[0030] Many methods known in the art are used to isolate and manipulate the DNA molecules or fragments thereof disclosed in this invention. For example, PCR (polymerase chain reaction) technology can be used to amplify specific starting DNA molecules and / or generate variants of the original molecules. DNA molecules or fragments thereof can also be obtained by other techniques, such as direct synthesis of fragments by chemical means, as is commonly performed using an automated oligonucleotide synthesizer.

[0031] As used herein, the term “sequence identity” refers to the degree of identity between two optimized sequence alignments of polynucleotide sequences or two optimized sequence alignments of polypeptide sequences. Optimized sequence alignments are generated by manually comparing two sequences, such as a reference sequence and another sequence, to maximize the number of nucleotide matches with appropriate internal nucleotide insertions, deletions, or gaps in the sequence alignment. As used herein, the term “reference sequence” refers to the sequence provided as the polynucleotide sequence in SEQ ID NO: 1-199, 211, and 212.

[0032] As used herein, the terms "percentage sequence similarity" or "% similarity" or "% similarity" are similarity scores multiplied by 100. The "similarity score" of a sequence optimized for comparison with a reference sequence is the number of nucleotide matches in the optimized comparison divided by the total number of nucleotides in the reference sequence (e.g., the total number of nucleotides in the entire reference sequence). Therefore, one embodiment of the invention comprises a DNA molecule, when optimized for comparison with reference sequences provided herein as SEQ ID NOs: 1-199, 211, and 212, a sequence having at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, or about 99% similarity to said reference sequences. In a particular embodiment, such a sequence may be defined as a peptide having gene regulatory activity or encoding a peptide that acts to localize an operablely linked polypeptide within a cell.

[0033] Control element

[0034] Regulatory elements are DNA molecules with gene regulatory activity, i.e., DNA molecules capable of influencing the transcription and / or translation of operably linked transcribed polynucleotide molecules. Therefore, the term "gene regulatory activity" refers to the ability to influence the expression pattern of operably linked transcribed polynucleotide molecules by affecting their transcription and / or translation. As used herein, a set of regulatory expression elements (EXP) for transcription may consist of operably linked expression elements such as enhancers, promoters, leader sequences, and introns. Thus, a set of regulatory expression elements for transcription may include, for example, a promoter operably linked to a 5′ to a leader sequence, which is then operably linked to a 5′ to an intron sequence. The intron sequence may consist of a sequence beginning at a point from a first intron / exon splice site in the natural sequence and may further consist of a small leader sequence fragment containing a second intron / exon splice site to provide appropriate intron / exon processing to facilitate transcription and appropriate processing of the resulting transcript. Leader sequences and introns can positively influence the transcription of operatively linked transcribed polynucleotide molecules and the translation of the resulting transcribed RNA. The pre-processed RNA molecule includes leader sequences and introns that can influence the post-transcriptional processing of the transcribed RNA and / or the export of the transcribed RNA molecule from the nucleus to the cytoplasm. Following post-transcriptional processing of the transcribed RNA molecule, the leader sequence can be retained as the final messenger RNA portion and can positively influence the translation of the messenger RNA molecule.

[0035] Regulatory elements, such as promoters, leader sequences, introns, and transcription termination regions, are DNA molecules that possess gene regulatory activity and act as integral parts of the overall gene expression in living cells. The term "regulatory element" refers to a DNA molecule with gene regulatory activity, that is, a DNA molecule capable of influencing the transcription and / or translation of transcribed polynucleotide molecules that are operatively linked. Therefore, isolated regulatory elements, such as promoters and leader sequences, that function in plants can be used to modify plant phenotypes through genetic engineering methods.

[0036] Regulatory elements can influence (qualitatively and / or quantitatively) their expression patterns, for example, through their temporal, spatial, developmental, tissue, environmental, physiological, pathological, cell cycle, and / or chemical response patterns and any combination thereof, as well as through positive or negative and / or fundamental or other effects by quantitative or qualitative indicators. Promoters serve as regulatory elements that regulate the expression of operatively linked, transcribed polynucleotide molecules.

[0037] As used herein, "gene expression pattern" refers to any pattern in which operatively linked DNA molecules are transcribed into transcribed RNA molecules. Transcribed RNA molecules can be translated to produce protein molecules or provide antisense or other regulatory RNA molecules, such as dsRNA, tRNA, rRNA, miRNA, etc.

[0038] As used herein, the term "protein expression" refers to any pattern of translating transcribed RNA molecules into protein molecules. Protein expression can be characterized by its temporal, spatial, developmental, or morphological properties, as well as quantitative or qualitative indicators.

[0039] As used herein, the term "promoter" generally refers to a DNA molecule involved in recognizing and binding RNA polymerase II and other proteins (trans-acting transcription factors) to initiate transcription. A promoter may first be isolated from the 5′ untranslated region (5′UTR) of a copy of the gene's genome. Optionally, a promoter may be a synthetically generated or manipulated DNA molecule. A promoter may also be chimeric, i.e., a promoter generated by the fusion of two or more heterologous DNA molecules. Promoters that can be used to implement the present invention include any one of SEQ ID NO: 2, 6, 8, 10, 12, 163, and 169, or promoter elements, fragments, or variants thereof, contained in any one of SEQ ID No: 13-199, 211, and 212. In specific embodiments of the invention, such molecules and any variants or derivatives thereof described herein are further defined as having promoter activity, i.e., the ability to function as a promoter in host cells, such as in transgenic plants. In a further specific embodiment, a fragment may be defined as exhibiting promoter activity of the initiator promoter molecule from which it is derived, or a fragment may contain a “minimum promoter” that provides a basic level of transcription and consist of a TATA box or equivalent sequence for recognizing and binding to the RNA polymerase II complex for transcription initiation.

[0040] In one embodiment, a fragment of a promoter molecule is provided. As described above, the promoter fragment provides promoter activity and can be used alone or in combination with other promoters and promoter fragments for, for example, to construct a chimeric promoter. In a specific embodiment, a promoter fragment comprising at least about 50, 95, 150, 250, 500, 750, or at least about 1000 consecutive nucleotides of a polynucleotide molecule having the promoter activities disclosed herein is provided.

[0041] Compositions derived from any promoter present in SEQ ID Nos: 2, 6, 8, 10, 12, 163, and 169, or promoter elements contained in SEQ ID Nos: 13-199, 211, and 212, such as internal or 5′ deletions, can be generated to enhance or alter expression, including by removing elements that have a positive or negative effect on expression; replicating elements that have a positive or negative effect on expression; and / or replicating or removing elements that have a tissue or cell-specific effect on expression. Compositions derived from any promoter present in SEQ ID Nos: 2, 6, 8, 10, 12, 163, and 169, or promoter elements contained in SEQ ID Nos: 13-199, 211, and 212 consisting of ′3 deletions, wherein the TATA box element or its equivalent and downstream sequences are removed, can be used, for example, to prepare enhancer elements. Further deletions can be generated to remove all elements that have a positive or negative effect on expression, a tissue-specific effect, a cell-specific effect, or a timing-specific effect (e.g., but not limited to, circadian rhythm). Any promoter present in SEQ ID No: 2, 6, 8, 10, 12, 163, and 169, or promoter elements included in SEQ ID No: 13-199, 211, and 212, and fragments or enhancers derived therefrom, can be used to prepare chimeric transcriptional regulatory element compositions comprising any promoter present in SEQ ID No: 2, 6, 8, 10, 12, 163, and 169, or promoter elements included in SEQ ID No: 13-199, 211, and 212, and fragments or enhancers derived therefrom operatively linking other enhancers and promoters. The efficacy of modifications, duplications, or deletions in the desired expression of specific transgenes described herein can be confirmed by empirical tests, such as those described in the working examples herein, in stable and transient plant experiments, which may vary depending on the changes made and the target of the alterations in the starting molecule.

[0042] As used herein, the term "leader sequence" refers to a DNA molecule isolated from the 5′ untranslated region (5′UTR) of a genomic copy of a gene and is generally defined as a nucleotide fragment between the transcription start site (TSS) and the protein-coding sequence start site. Alternatively, the leader sequence may be a synthetically generated or manipulated DNA element. The leader sequence can serve as a 5′ regulatory element for regulating the expression of operatively linked, transcribed polynucleotide molecules. Leader sequence molecules can be used with heterologous promoters or with their native promoters. Thus, the promoter molecules of the present invention can be operatively linked with their native leader sequences or can be operatively linked with heterologous leader sequences. Leader sequences that can be used to implement the present invention include SEQ ID NO: 3, 164, 166, and 170, or leader sequence elements, fragments, or variants thereof contained in SEQ ID No: 13-199, 211, and 212. In specific embodiments, such sequences can be provided as capable of serving as leader sequences in host cells, such as transgenic plant cells. In one embodiment, such sequences are decoded to contain leader sequence activity.

[0043] The leader sequences (5′UTRs) present in SEQ ID Nos: 3, 164, 166, and 170, or the leader sequence elements included in any one of SEQ ID Nos: 13-199, 211, and 212, may consist of regulatory elements or may employ secondary structures capable of influencing the transcription or translation of transgenes. The leader sequences present in SEQ ID Nos: 3, 164, 166, and 170, or the leader sequence elements included in any one of SEQ ID Nos: 13-199, 211, and 212, can be used according to the present invention to prepare chimeric regulatory elements influencing the transcription or translation of transgenes. Furthermore, the leader sequences present in SEQ ID Nos: 3, 164, 166, and 170, or the leader sequence elements included in any one of SEQ ID Nos: 13-199, 211, and 212, can be used to prepare chimeric leader sequences influencing the transcription or translation of transgenes.

[0044] The introduction of exogenous genes into new plant hosts does not always lead to high expression of the introduced gene. Furthermore, when dealing with complex traits, it is sometimes necessary to regulate multiple genes with spatially or temporally different expression patterns. Introns can primarily provide this regulation. However, it has been shown that the multiple uses of the same intron in a transgenic plant are insufficient. In these cases, a set of basic control elements for constructing appropriate recombinant DNA elements is required. Since the existing set of introns known in the art for expression enhancement is limited, alternatives are needed.

[0045] Compositions derived from any intron present in SEQ ID Nos: 4, 165, and 171, or intronic elements contained in SEQ ID Nos: 13-199, 211, and 212, may consist of internal deletions or duplications of cis-regulatory elements; and / or when operable to link promoter + leader sequences or chimeric promoter + leader sequences and coding sequences, alterations to the 5′ and 3′ sequences containing the intron / exon splicing site may be used to improve expression or expression specificity. Alterations to the 5′ and 3′ regions containing the intron / exon splicing site may also be made to reduce the likelihood of the introduction of false start and stop codons in the resulting transcript after messenger RNA processing and splicing. The introns may be empirically tested as described in the working examples to determine their effect on transgene expression.

[0046] According to the present invention, the presence of known promoter elements, i.e., DNA sequence characteristics such as TATA boxes and other known transcription factor binding site motifs, can be analyzed in the promoter or promoter fragment. Those skilled in the art can use this identification of known promoter elements to design variants with expression patterns similar to the original promoter.

[0047] As used herein, the term "enhancer" or "enhancer element" refers to a cis-acting transcriptional regulatory element, also known as a cis-element, which provides one aspect of the overall expression pattern but is generally insufficient to drive the transcription of operably linked polynucleotide sequences on its own. Unlike promoters, enhancer elements typically do not include a transcription start site (TSS) or TATA box or equivalent sequence. Promoters may naturally contain one or more enhancer elements that influence the transcription of operably linked polynucleotide sequences. Isolated enhancer elements may also be fused to promoters to produce chimeric promoter cis-elements, which provide one aspect of the overall regulation of gene expression. Promoters or promoter fragments may contain one or more enhancer elements that influence the transcription of operably linked genes. Many promoter enhancer elements are believed to bind DNA-binding proteins and / or influence DNA topology, creating local constructs that selectively allow or restrict RNA polymerase access to the DNA template or promote selective opening of the double helix at the transcription start site. Enhancer elements may function to bind transcription factors that regulate transcription. Some enhancer elements bind to more than one transcription factor, and transcription factors can interact with more than one enhancer domain through varying affinities. Enhancer elements can be identified using a variety of techniques, including deletion analysis (i.e., deletion of one or more nucleotides from or within the 5′ end of the promoter); DNA-binding protein analysis using DNase I footprinting, methylation interference, electrophoretic mobility shift assays, in vivo genomic footprinting via ligation-mediated PCR, and other conventional assays; or DNA sequence similarity analysis using known cis-element motifs or enhancer elements as target sequences or target motifs in conjunction with conventional DNA sequence comparison methods such as BLAST. The fine structure of enhancer domains can be further investigated through mutation (or substitution) of one or more nucleotides or through other conventional methods. Enhancer elements can be obtained through chemical synthesis or isolated from regulatory elements that include such elements, and they can be synthesized together with additional flanking nucleotides containing useful restriction enzyme sites to facilitate subsequent manipulation. Therefore, this invention covers the design, construction, and use of enhancer elements according to the methods disclosed herein for regulating the expression of operably linked, transcribed polynucleotide molecules.

[0048] In plants, the presence of some introns in gene constructs, relative to constructs lacking these introns, leads to increased mRNA and protein accumulation. This effect has been termed "intron-mediated enhancement" (IME) of gene expression (Mascarenhas et al., (1990) Plant Mol. Biol. 15: 913-920). In maize genes (e.g., tubA1, Adh1, Sh1, Ubi1 (Jeon et al., (2000) Plant Physiol. 123: 1005-1014; Callis et al., (1987) Genes Dev. 1: 1183-1200; Vasil et al., (1989) Plant Physiol. 91: 1575-1579; Christiansen et al., (1992) Plant Mol. Biol. 18: 675-689) and in rice genes (e.g., salt, tpi: McElroy et al., Plant Cell 2: 163-171 (1990); Xu et al., Plant Physiol. 106: 459-467 (1994) has identified introns known to stimulate expression in plants. Similarly, introns from dicotyledonous genes, such as those from petunia (e.g., rbcS), potatoes (e.g., st-ls1), and Arabidopsis (e.g., ubq3 and pat1), have been found to increase gene expression rates (Dean et al., (1989) Plant Cell 1: 201-208; Leon et al., (1991) Plant Physiol. 95: 968-972; Norris et al., (1993) Plant Mol Biol 21: 895-906; Rose and Last (1997) Plant J. 11: 455-464). Deletion or mutation within the splice site of an intron has been shown to reduce gene expression, suggesting that IME may require splicing (Mascarenhas et al., (1990) Plant Mol). Biol. 15: 913-920; Clancy and Hannah, (2002) Plant Physiol. 130: 918-929). However, point mutations within the splice site of the pat1 gene from Arabidopsis have shown that a certain IME in dicotyledonous plants does not require splicing itself (Rose and Beliakoff (2000) Plant Physiol. 122: 535-542).

[0049] Enhanced gene expression via introns is not a common phenomenon because some introns inserted into recombination expression cassettes do not enhance expression (e.g., introns from dicotyledonous genes (rbcS from peas, lentil protein from beans, and stls-1 from potatoes) and introns from maize genes (ninth intron of adh1 and first intron of hsp81)) (Chee et al., (1986) Gene 41: 47-57; Kuhlemeier et al., (1988) Mol Gen Genet 212: 405-411; Mascarenhas et al., (1990) Plant Mol. Biol. 15: 913-920; Sinibaldi and Mettler (1992) In WE Cohn, K Moldave, eds, Progress in Nucleic Acid Research and Molecular Biology, Vol 42. Academic Press, New York, pp. 229-257; Vancanneyt et al., 1990 Mol. Gen. Genet. 220: 245-250). Therefore, not every intron can be used to manipulate the gene expression levels of non-endogenous or endogenous genes in transgenic plants. What characteristics or specific sequence features must be present in the intron sequence to enhance the expression rate of a given gene is unknown in the prior art; therefore, when used heterologously, it is impossible to predict whether a given plant intron will induce IME based on the prior art.

[0050] As used herein, the term "chimeric" refers to a single DNA molecule generated by fusing a first DNA molecule with a second DNA molecule, wherein neither the first nor the second DNA molecule is typically found in this configuration, i.e., in fused configurations. Therefore, a chimeric DNA molecule is a novel DNA molecule rather than one typically found in nature. As used herein, the term "chimeric promoter" refers to a promoter generated by performing this operation on a DNA molecule. Chimeric promoters can bind two or more DNA fragments; an example is the fusion of a promoter with an enhancer element. Therefore, this invention covers the design, construction, and use of chimeric promoters according to the methods disclosed herein for regulating the expression of operatively linked, transcribed polynucleotide molecules.

[0051] As used herein, the term "variant" refers to a second DNA molecule that is compositionally similar to but not identical to a first DNA molecule, while retaining conventional functionality, i.e., having the same or similar expression pattern as the first DNA molecule. A variant can be a shorter or truncated form of the first DNA molecule and / or a variation of the first DNA molecule's sequence, such as a DNA molecule with different restriction enzyme sites and / or internal deletions, substitutions, and / or insertions. A "variant" can also include a regulatory element having a nucleotide sequence of substitutions, deletions, and / or insertions comprising one or more nucleotides of a reference sequence, wherein the derived regulatory element has stronger or weaker or equal transcriptional or translational activity than the corresponding parent regulatory molecule. The regulatory element "variants" can also encompass variants resulting from mutations naturally occurring in bacterial and plant cell transformations. In this invention, the polynucleotide sequences provided with SEQ ID Nos: 1-199, 211, and 212 can be used to generate variants that are compositionally similar to but not identical to the original regulatory element, while retaining conventional functionality, i.e., having the same or similar expression pattern as the original regulatory element. Such variant generation is well known to those skilled in the art based on this disclosure and is included within the scope of this invention. The "variant" of the chimeric regulatory element comprises constituent elements identical to the reference chimeric regulatory element sequence, but can be operatively linked by various methods known in the art, such as restriction enzyme digestion and ligation, cloning-independent ligation, modular assembly of PCR products during amplification, or direct chemical synthesis of the chimeric regulatory element, and other methods known in the art. The resulting "variant" chimeric regulatory element consists of constituent elements identical to the reference sequence but different in terms of one or more sequences used for operatively linking the constituent elements, or variations thereof. In this invention, reference sequences are provided for each of the polynucleotide sequences provided in SEQ ID Nos: 1-199, 211, and 212, wherein the constituent elements of said reference sequences can be linked by methods known in the art and can consist of one or more nucleotides naturally present in bacterial and plant cell transformations, or substitutions, deletions, and / or insertions of mutations.

[0052] Construct

[0053] As used herein, the term "construct" refers to any recombinant polynucleotide molecule derived from any source, capable of genome integration or autonomous replication, and containing a polynucleotide molecule (one or more of which are functionally or operationally linked), such as plasmids, granules, viruses, autonomously replicating polynucleotide molecules, bacteriophages, or linear or circular single- or double-stranded DNA or RNA polynucleotide molecules. As used herein, the term "vector" refers to any recombinant polynucleotide construct that can be used for transformation purposes (i.e., introducing heterologous DNA into host cells). The terms include expression cassettes isolated from any of the above-described molecules.

[0054] As used herein, the term "operably linked" means that a first molecule is linked to a second molecule, wherein the molecules are arranged such that the first molecule influences the function of the second molecule. The two molecules may or may not be part of a single, continuous molecule, and may be adjacent or not adjacent. For example, if a promoter regulates the transcription of a target transcribed polynucleotide molecule in a cell, then the promoter is operably linked to the transcribed polynucleotide molecule. For example, when a leader sequence is capable of being used as a leader sequence for a polypeptide encoded by a coding sequence, it is operably linked to the coding sequence.

[0055] In one embodiment, the construct of the present invention can be provided as a double Ti plasmid border DNA construct having right border (RB or AGRtu.RB) and left border (LB or AGRtu.LB) regions of a Ti plasmid isolated from *Agrobacterium tumefaciens* containing T-DNA, together with a transformation molecule provided by *Agrobacterium tumefaciens* cells, allowing T-DNA integration into the genome of plant cells (see, for example, U.S. Patent 6,603,061). The construct may also include plasmid backbone DNA fragments that provide replication function and antibiotic selectivity in bacterial cells, such as *E. coli* origin of replication like ori322, broad host-range origin of replication like oriV or oriRi, and coding regions encoding Tn7 aminoglycoside adenyl transferase (aadA) to provide selectable markers of resistance to spectinomycin or streptomycin like Spec / Strp or gentamicin (Gm, Gent) selectable marker genes. For plant transformation, the host bacterial strains are typically Agrobacterium tumefaciens ABI, C58, or LBA4404; however, other strains known in the field of plant transformation can also function in this invention.

[0056] Methods for assembling and introducing constructs into cells in a manner that transcribes transcribed polynucleotide molecules into functional mRNA molecules that are translated and expressed as protein products are known. For the practice of this invention, conventional compositions and methods for preparing and using constructs and host cells can be found, for example, *Molecular Cloning: A Laboratory Manual, 3rd edition Volumes 1, 2, and 3* (2000), J.F. Sambrook, D.W. Russelell, and N. Irwin, ColdSpring Harbor Laboratory Press. Methods for preparing recombinant vectors particularly suitable for plant transformation include, but are not limited to, those described in their entirety in U.S. Patents 4,971,908, 4,940,835, 4,769,061, and 4,757,011. These types of vectors have also been reviewed in the scientific literature (see, for example, Rodriguez et al., Vectors: A Survey of Molecular Cloning Vectors and Their Uses, Butterworths, Boston, (1988) and Glick et al., Methods in Plant Molecular Biology and Biotechnology, CRC Press, Boca Raton, FL. (1993)). Typical vectors for expressing nucleic acids in higher plants are well known in the art and include vectors derived from tumor-inducing (Ti) plasmids of *Agrobacterium tumefaciens* (Rogers et al., Methods in Enzymology, 153: 253-277 (1987)). Other recombinant vectors for plant transformation, including the pCaMVCN transformation control vector, have also been described in the scientific literature (see, for example, Fromm et al., Proc. Natl. Acad. Sci. USA, 82: 5824-5828 (1985)).

[0057] Any of the constructs provided herein may include different regulatory elements. Any such regulatory element may be provided in combination with other regulatory elements. Such combinations may be designed or modified to produce desired regulatory properties. In one embodiment, the constructs of the present invention comprise at least one regulatory element operatively linked to a transcribed polynucleotide molecule operatively linked to a 3′ transcription termination molecule.

[0058] The constructs of this invention may include any promoter or leader sequence provided herein or known in the art. For example, the promoters of this invention may be operatively linked to a heterologous untranslated 5′ leader sequence, such as a heterologous untranslated 5′ leader sequence derived from a heat shock protein gene (see, for example, U.S. Patent Nos. 5,659,122 and 5,362,865). Alternatively, the leader sequence of this invention may be operatively linked to a heterologous promoter such as the cauliflower mosaic virus 35S transcription promoter (see, U.S. Patent No. 5,352,605). The expression properties conferred by such operative linking of heterologous elements are not necessarily a superposition of the properties elucidated by each promoter and leader sequence, but are determined through empirical analysis of expression driven by operatively linked heterologous promoters and leader sequences.

[0059] As used herein, the term "intron" refers to a DNA molecule that can be isolated or identified from a genomic copy of a gene and is generally defined as a region of DNA spliced ​​during pre-translational mRNA processing. Alternatively, an intron can be a synthetically generated or manipulated DNA element. Introns may contain enhancer elements that influence the transcription of operably linked genes. Introns can be used as regulatory elements to regulate the expression of operably linked transcribed polynucleotide molecules. DNA constructs may contain introns, which may or may not be heterologous to the sequence of the transcribed polynucleotide molecule. Examples of introns in the art include the rice actin intron (US Patent No. 5,641,876) and the maize HSP70 intron (US Patent No. 5,859,347). Introns useful for carrying out the present invention include intronic elements contained in any one of SEQ ID Nos: 4, 165, and 171.

[0060] As used herein, the term "3′ transcription termination molecule" or "3′UTR" refers to the DNA molecule used during transcription to generate the 3′ untranslated region (3′UTR) of an mRNA molecule. The 3′ UTR of an mRNA molecule can be generated through specific cleavage and 3′ polyadenylation (i.e., poly-A tail). The 3′UTR can be operatively linked to and located downstream of a transcribed polynucleotide molecule and may include polynucleotides that provide polyadenylation signals and other regulatory signals that can influence transcription, mRNA processing, or gene expression. The poly-A tail is thought to play a role in mRNA stability and the initiation of translation. Examples of 3′ transcription termination molecules include the 3′ region of carmine synthase (see Fraley et al., Proc. Natl. Acad. Sci. USA, 80: 4803-4807 (1983)); the 3′ region of wheat hspl7; the 3′ region of the small subunit of pea ribulose diphosphate carboxylase-oxygenase; the 3′ region of cotton E6 (US Patent 6,096,950); the 3′ region disclosed in WO0011200A2; and the 3′ UTR of coixol (US Patent No. 6,635,806).

[0061] 3′UTRs are often found to have advantageous uses for specific gene recombination expression. In animal systems, the mechanisms of 3′UTRs are well understood (e.g., Zhao et al., Microbiol Mol Biol Rev 63:405-445 (1999); Proudfoot, Nature 322:562-565 (1986); Kim et al., Biotechnology Progress 19:1620-1622 (2003); Yonaha and Proudfoot, EMBO J.19:3770-3777 (2000); Cramer et al., FEBS Letters 498:179-182 (2001); Kuerstem and Goodwin, Nature Reviews Genetics 4:626-637 (2003)). Effective termination of RNA transcription is required to prevent the transcription of unwanted, trait-irrelevant (downstream) sequences (which interfere with trait expression). Compared to irrelevant insertions, the arrangement of multiple gene expression cassettes in local proximity to each other (e.g., within a single T-DNA) can lead to suppression of gene expression of one or more genes within the construct (Padidam and Cao, BioTechniques 31:328-334 (2001)). This can, for example, prevent the achievement of adequate expression levels in cases where strong gene expression from all cassettes is desired.

[0062] In plants, well-defined polyadenylation signal sequences are unknown. Hasegawa et al., Plant J. 33: 1063-1072, (2003) were unable to identify conserved polyadenylation signal sequences in in vitro and in vivo systems in forest tobacco (Nicotiana sylvestris) and determine the actual length of primary (non-polyadenylated) transcripts. Weak 3′UTRs have the potential to generate read-through, which can affect gene expression in adjacent expression cassettes (Padidam and Cao, BioTechniques 31: 328-334 (2001)). Proper control of transcription termination can prevent read-through in downstream sequences (e.g., other expression cassettes) and can further allow efficient recycling of RNA polymerase, thereby enhancing gene expression. Efficient termination of transcription (release of RNA polymerase from DNA) is essential for the restart of transcription and thus directly affects the entire transcript level. Following transcription termination, mature mRNA is released from the cytoplasm and template site. Eukaryotic mRNA accumulates in vivo in multiple (A) forms, making it difficult to detect transcription termination sites using conventional methods. However, predicting the functional and effective 3′UTR using bioinformatics methods is challenging because there are no conserved sequences that make the effective 3′UTR easily predictable.

[0063] From a practical point of view, the 3′UTR used in transgenic cassettes generally has the following characteristics that are advantageous. The 3′UTR will efficiently and effectively terminate the transcription of the transgene and prevent the transcript from reading through any adjacent DNA sequence or adjacent chromosome DNA where the T-DNA has been inserted, such that in the case of multiple cassettes remaining in a single T-DNA, the adjacent DNA sequence may be composed of another transgenic cassette. The 3′UTR should not cause a reduction in the transcriptional activity conferred by the promoter, leader sequence, and introns used to drive transgene expression. In plant biotechnology, the 3′UTR is commonly used to initiate the amplification reaction of reverse-transcribed RNA extracted from the transformed plant and to (1) assess the transcriptional activity or expression of the transgenic cassette that has been integrated into the plant chromosome; (2) assess the copy number inserted into the plant DNA; and (3) assess the conjugation of seeds formed after breeding. The 3′UTR is also used for the amplification reaction of DNA extracted from the transformed plant to characterize the integrity of the inserted cassette.

[0064] The expression of sequence tags (ESTs) expressed in cDNA libraries composed of messenger RNAs derived from seeds, flowers, and other tissues of millet (Setaria italica (L.) Beauv) can identify 3′UTRs useful for providing transgenic expression in plants. The cDNA libraries consist of tissues isolated from selected plant varieties using flower tissues, seeds, leaves, and roots. The obtained cDNAs were sequenced using various sequencing methods. The obtained ESTs were assembled into clusters using bioinformatics software such as clc_ref_assemble_complete version 2.01.37139 (CLC bio USA, Cambridge, Massachusetts 02142). Transcript abundance for each cluster was determined by counting the number of cDNA reads in each cluster. The identified 3′UTRs can consist of sequences derived from both cDNA and genomic DNA. The cDNA sequences were used to design primers, which were then coupled to GenomeWalker primers constructed according to the manufacturer's specifications. TM (Clontech Laboratories, Inc., Mountain View, CA) libraries are used together to clone the 3′ region of the corresponding genomic DNA sequence to provide a longer termination sequence. Relative transcript abundance analysis of direct or normalized counts of sequence reads observed in each tissue library can be used to infer characteristics regarding expression patterns. For example, some 3′UTRs can be found in transcripts with higher abundance observed in root tissues, contrary to leaves. This indicates that the transcript is highly expressed in roots, and that the characteristic of root expression can be attributed to transcriptional regulation by promoters, leader sequences, introns, or 3′UTRs. Empirical tests to identify 3′UTRs based on the characteristics of expression in specific organs, tissues, or cell types can lead to the identification of 3′UTRs with enhanced expression in those specific organs, tissues, or cell types.

[0065] The construct and vector may also include a transport peptide encoding sequence expressing a linked peptide class for targeting protein products, particularly chloroplasts, leucoplasts, or other plastid organelles; mitochondria; peroxisomes; vacuoles, or extracellular sites. For a description of the use of chloroplast transport peptides, see U.S. Patent Nos. 5,188,642 and 5,728,925. Many chloroplast-localized proteins are expressed as precursors from nuclear genes and target chloroplasts via chloroplast transport peptides (CTPs). Examples of such isolated chloroplast proteins include, but are not limited to, those described in U.S. Patent No. 7,193,133 related to the small subunit (SSU) of ribulose-1,5'-bisphosphate carboxylase, ferricredoxin, ferricredoxin oxidoreductase, light-harvesting complex proteins I and II, thioredoxin F, enolpyruvate shikimate phosphate synthase (EPSPS), and transport peptides. It has been demonstrated in vivo and in vitro that non-chloroplast proteins can be targeted to chloroplasts by using protein fusion with a heterologous CTP, and that the CTP is sufficient to target the protein to the chloroplast. The introduction of suitable chloroplast transport peptides such as Arabidopsis EPSPS CTP (CTP2) (see, Klee et al., Mol. Gen. Genet., 210: 437-442 (1987)) or Petunia EPSPS CTP (CTP4) (see, della-Cioppa et al., Proc. Natl. Acad. Sci. USA, 83: 6873-6877 (1986)) has been shown to target heterologous EPSPS protein sequences to chloroplasts in transgenic plants (see, U.S. Patent Nos. 5,627,061, 5,633,435 and 5,312,910 and EP 0218571, EP 189707, EP508909 and EP 924299).

[0066] Transcribed polynucleotide molecules

[0067] As used herein, the term "transcribed polynucleotide molecule" means any DNA molecule capable of being transcribed into an RNA molecule, including but not limited to those having protein-coding sequences and those generating RNA molecules having sequences for gene repression. "Transgenic" means a transtranscribed polynucleotide molecule that is at least heterologous to the host cell in terms of its position in the genome and / or a transtranscribed polynucleotide molecule artificially introduced into the host cell genome in present or any previous generation of cells.

[0068] The promoter of the present invention can be operatively linked to a transcribed polynucleotide molecule that is heterologous to the promoter molecule. As used herein, the term "heterologous" refers to a combination of two or more polynucleotide molecules when such a combination is not normally present in nature. For example, the two molecules may be derived from different species and / or the two molecules may be derived from different genes, for example, different genes from the same species or the same gene from different species. Therefore, if such a combination is not normally present in nature, the promoter is heterologous to the operatively linked transcribed polynucleotide molecule, i.e., the combination of the transcribed polynucleotide molecule and the promoter molecule is not naturally occurring and operatively linked.

[0069] Transcribed polynucleotide molecules can generally be any DNA molecule required to express an RNA transcript. This expression of the RNA transcript leads to the translation of the resulting mRNA molecule and the expression of the protein. Optionally, for example, the transcribed polynucleotide molecule can be designed to ultimately cause a reduction in the expression of a specific gene or protein. In one embodiment, this can be achieved by using a transcribed polynucleotide molecule oriented in an antisense direction. In short, when an antisense transcribed polynucleotide molecule is transcribed, the RNA product hybridizes with and sequesters the complementary RNA molecule in the cell. This double-helix RNA molecule cannot be translated into protein by the cell's translational mechanisms and is degraded in the cell. Any gene can be negatively regulated in this way.

[0070] Therefore, one embodiment of the invention is a regulatory element of the invention, such as those provided with SEQ ID NO: 1-199, 211, and 212, which is operatively linked to a transcribed polynucleotide molecule such that when said construct is introduced into the genome of a plant cell, the transcription of the transcribed polynucleotide molecule is regulated at a desired level or in a desired pattern. In one embodiment, the transcribed polynucleotide molecule comprises a protein-coding region of a gene, and the promoter influences the transcription of an RNA molecule that is translated and expressed as a protein product. In another embodiment, the transcribed polynucleotide molecule comprises an antisense region of a gene, and the promoter influences the transcription of antisense RNA molecules, double-stranded RNA, or other similar repressive RNA molecules to inhibit the expression of a target-specific RNA molecule in the target host cell.

[0071] Agronomic Genes

[0072] Transcribed polynucleotide molecules can be agronomically targeted genes. As used herein, the term "agronomically targeted gene" refers to a transcribed polynucleotide molecule that, when expressed in a specific plant tissue, cell, or cell type, confers desired characteristics related to plant morphology, physiology, growth, development, yield, product, nutrient distribution, disease or pest resistance, and / or environmental or chemical tolerance. Agronomically targeted genes include, but are not limited to, those encoding yield proteins, stress resistance proteins, developmental control proteins, tissue differentiation proteins, meristematic proteins, environmental response proteins, senescence proteins, hormone response proteins, abscission proteins, source proteins, sink proteins, flower control proteins, seed proteins, herbicide resistance proteins, disease resistance proteins, fatty acid biosynthesizers, tocopherol biosynthesizers, amino acid biosynthesizers, insecticidal proteins, or any other agent, such as targeting antisense or RNAi molecules that inhibit a specific gene. The products of agronomically targeted genes can function within plants to influence plant physiology or metabolism, or can function as insecticides as food for plant-feeding pests.

[0073] In one embodiment of the invention, the promoter of the invention is introduced into the construct such that the promoter is operatively linked to a transcribed polynucleotide molecule serving as an agronomic target gene. Expression of the agronomic target gene is desired to confer beneficial agronomic traits. Beneficial agronomic traits may include, for example, but not limited to, herbicide tolerance, insect control, improved yield, fungal disease resistance, viral resistance, nematode resistance, bacterial disease resistance, plant growth and development, starch production, improved oil production, high oil production, improved fatty acid content, high protein production, fruit ripening, improved animal and human nutrition, biopolymers, environmental stress resistance, medicinal peptides and secretible peptides, improved processing traits, improved digestibility, enzyme production, flavor, nitrogen fixation, hybrid seed production, fiber production, and biofuel production. Examples of agronomically targeted genes include those exhibiting herbicide resistance (US Patent Nos. 6,803,501, 6,448,476, 6,248,876, 6,225,114, 6,107,549, 5,866,775, 5,804,425, 5,633,435, and 5,463,175) and increased yield (US Patent Nos. USRE38,446, 6,716,474, 6,663,906, 6,476,295, 6,441,277, and 6,000). ,423,828, 6,399,330, 6,372,211, 6,235,971, 6,222,098 and 5,716,837), insect control (US Patent Nos. 6,809,078, 6,713,063, 6,686,452, 6,657,046, 6,645,497, 6,642,030, 6,639,054, 6,620,988, 6,593,293, 6,555,655, 6,538,109, 6,537,756, 6,521,442, 6,501,009, 6,468,523, 6,326,351, 6,313,378, 6,284,949, 6,281,016, 6,248,536, 6,242,241, 6,221,649, 6,177,615, 6,156,573, 6,153,814, 6,110,464, 6,093,695, 6,063,756, 6,063,597 6,023,013, 5,959,091, 5,942,664, 5,942,658, 5,880,275, 5,763,245 and 5,763,241), fungal disease resistance (US Patent Nos. 6,653,280, 6,573,361, 6,506,962, 6,316,407, 6,215,048, 5,516,671, 5,773,696, 6,121,436, 6,316,407 and 6,506,962), virus resistance (US Patent Nos. 6,617,496, 6,608,241, 6,015,940, 6,013,864, 5,850,023 and 5,304,730), nematode resistance (US Patent No. 6,228,992), bacterial disease resistance (US Patent No. 5,516,671), plant growth and development (US Patent Nos. 6,723,897 and 6,518,488), starch production (US Patent Nos. 6,538,181, 6,538,179, 6,538,178, 5,750,876, 6,476,295), improved oil production (US Patent Nos. 6,617,496, 6,608,241, 6,015,940, 6,013,864, 5,850,023 and 5,304,730), US Patent Nos. 6,444,876; 6,426,447 and 6,380,462), high oil production (US Patent Nos. 6,495,739, 5,608,149, 6,483,008 and 6,476,295), improved fatty acid content (US Patent Nos. 6,828,475, 6,822,141, 6,770,465, 6,706,950, 6,660,849, 6,596,538, 6,589,767, 6,537,750, 6,489,461 and 6,459,018), and high protein production (US Patent No. 6,380,466). Fruit ripening (US Patent No. 5,512,466), improved animal and human nutrition (US Patent Nos. 6,723,837, 6,653,530, 6,5412,59, 5,985,605, and 6,171,640), biopolymers (US Patent Nos. USRE 37,543, 6,228,623, 5,958,745, and 6,946,588), environmental stress resistance (US Patent No. 6,072,103), medicinal peptides and secretible peptides (US Patent Nos. 6,812,379, 6,774,283, 6,140,075, and 6,080,560), and improved Processing properties (US Patent No. 6,476,295), improved digestibility (US Patent No. 6,531,648), low raffinose (US Patent No. 6,166,292), industrial enzyme production (US Patent No. 5,543,576), improved flavor (US Patent No. 6,011,199), nitrogen fixation (US Patent No. 5,229,114), hybrid seed production (US Patent No. 5,689,041), fiber production (US Patent Nos. 6,576,818, 6,271,443, 5,981,834, and 5,869,720), and biofuel production (US Patent No. 5,998,700).

[0074] Optionally, the agronomic target gene can influence the aforementioned plant characteristics or phenotypes by encoding a targeted regulatory RNA molecule that induces gene expression of an endogenous gene, for example, through antisense (see, for example, U.S. Patent 5,107,065), repressive RNA (“RNAi”, including gene expression regulated by miRNA-, siRNA-, trans-acting siRNA-, and phased-controlled sRNA-mediated mechanisms, for example, as described in published applications US2006 / 0200878 and US2008 / 0066206 and U.S. Patent Application 11 / 974,469), or co-repressive-mediated mechanisms. The RNA can also be a catalytic RNA molecule engineered to cleave the desired endogenous mRNA product (e.g., a ribozyme or riboswitch; see, for example, US2006 / 0200878). Therefore, any transcribed polynucleotide molecule encoding a transcribed RNA molecule that influences agronomically important phenotypes or target morphological changes can be used to implement the present invention. Methods for constructing constructs and introducing them into cells by transcribing transcribed polynucleotide molecules into molecules capable of inducing gene repression are known in the art. For example, post-transcriptional gene repression using constructs and antisense-directed transcribed polynucleotide molecules to regulate gene expression in plant cells is disclosed in U.S. Patent Nos. 5,107,065 and 5,759,829, and post-transcriptional gene repression using constructs and sense-directed transcribed polynucleotide molecules to regulate gene expression in plants is disclosed in U.S. Patent Nos. 5,283,184 and 5,231,020. Expression of transcribed polynucleotides in plant cells can also be used to repress plant pests that consume plant cells, such as compositions isolated from coleopteran pests (U.S. Patent Publication No. US20070124836) and compositions isolated from nematode pests (U.S. Patent Publication No. US20070250947). Plant pests include, but are not limited to, arthropod pests, nematode pests, and fungal or microbial pests. Exemplary transcribed polynucleotide molecules incorporating the constructs of this invention include, for example, DNA molecules or genes from species different from the target species, or genes originating in or present in the same species but introduced into recipient cells through genetic engineering methods rather than conventional propagation or breeding techniques. Types of polynucleotide molecules include, but are not limited to, polynucleotide molecules already present in plant cells, polynucleotide molecules from another plant, polynucleotide molecules from different organisms, or externally generated polynucleotide molecules, such as polynucleotide molecules containing antisense genetic messages, or polynucleotide molecules encoding artificial, synthetic, or other modified forms of transgenic genes.

[0075] Selective tagging

[0076] As used herein, the term "marker" refers to any transcribed polynucleotide molecule whose expression or non-expression can be screened or scored in some way. Marker genes used in carrying out the present invention include, but are not limited to, transcribed polynucleotide molecules encoding β-glucuronidase (GUS as described in U.S. Patent No. 5,599,670), green fluorescent protein and its variants (GFP as described in U.S. Patent Nos. 5,491,084 and 6,146,826), proteins conferring antibiotic resistance, or proteins conferring herbicide resistance. Available antibiotic resistance markers include those encoding proteins conferring resistance to kanamycin (nptII), hygromycin B (aph IV), streptomycin or spectinomycin (aad, spec / strep), and gentamicin (aac3 and aacC4). Herbicides that have been proven to be tolerant to transgenic plants and can be applied to the methods of this invention include, but are not limited to: amino-methylphosphonic acid, glyphosate, glufosinate, sulfonylurea, imidazolinone, bromobenzonitrile, delapon, dicamba, cyclohexanedione, protoporphyrinogen oxidase inhibitors, and isoxaflutole herbicides. Transcribed polynucleotide molecules encoding proteins involved in herbicide tolerance include, but are not limited to, transcribed polynucleotide molecules encoding 5-enolpyruvate shikimate-3-phosphate synthase (EPSPS for glyphosate tolerance described in U.S. Patent Nos. 5,627,061, 5,633,435, 6,040,497, and 5,094,945); transcribed polynucleotide molecules encoding glyphosate oxidoreductase and glyphosate-N-acetyltransferase (GOX described in U.S. Patent No. 5,463,175, GAT described in U.S. Patent Publication No. 20030083480, and dicamba monooxygenase in U.S. Patent Publication No. 20030135879); transcribed polynucleotide molecules encoding bromobenzonitrile hydrolase (Bxn for bromobenzonitrile tolerance described in U.S. Patent No. 4,810,648); as described in Misawa et al., Plant Journal. Transcribed polynucleotide molecules encoding phytohexene desaturase (crtI) for tolerance to dapoxetine are described in 4:833-840 (1993) and Misawa et al., Plant Journal 6:481-489 (1994); transcribed polynucleotide molecules encoding acetylhydroxy acid synthase (AHAS, also known as ALS) for tolerance to sulfonylurea herbicides are described in Sathasiivan et al., Nucl. Acids Res. 18:2188-2193 (1990); and bar genes for tolerance to glufosinate and phosmet are described in DeBlock et al., Emb EMBO Journal 6:2513-2519 (1987).The promoter molecule of this invention can express a transcribed polynucleotide molecule that encodes phosphinothricin acetyltransferase, glyphosate-resistant EPSPS, aminoglycoside phosphotransferase, hydroxyphenylpyruvate dehydrogenase, hygromycin phosphotransferase, neomycin phosphotransferase, sphagnum dehalogenase, bromobenzonitrile-resistant nitrile hydrolase, anthranilate synthase, aryloxyalkylene ester dioxygenase, acetyl-CoA carboxylase, glyphosate oxidoreductase, and glyphosate-N-acetyltransferase.

[0077] Included in the term "selective marker" are genes encoding secretible markers, the secretion of which can serve as a means of identifying or selecting transformed cells. Examples include markers encoding secretible antigens that can be identified through antibody interactions or even markers encoding secretible enzymes that can catalyze detection. Selectively secreted marker proteins fall into several categories, including small, diffusible proteins that are detectable (e.g., by ELISA), small active enzymes that can be detected in extracellular solution (e.g., α-amylase, β-lactamase, glufosinate-transferase), or proteins that are inserted into or embedded in the cell wall (e.g., proteins including leader sequences found, for example, in extended expression units or tobacco-pathogenic proteins also known as tobacco PR-S). Other possible selective marker genes will be apparent to those skilled in the art and are covered herein.

[0078] Cell transformation

[0079] The present invention also relates to a method for producing transformed cells and plants comprising promoters operatively linked to transcribed polynucleotide molecules.

[0080] The term “transformation” refers to the introduction of nucleotides into a recipient host. As used herein, the term “host” refers to bacteria, fungi, or plants, including any cell, tissue, organ, or progeny of bacteria, fungi, or plants. Specific target plant tissues and cells include protoplasts, callus, roots, tubers, seeds, stems, leaves, seedlings, embryos, and pollen.

[0081] As used herein, the term "transformed" refers to a cell, tissue, organ, or organism in which a foreign polynucleotide molecule, such as a construct, has been introduced. The introduced polynucleotide molecule can be integrated into the genomic DNA of the recipient cell, tissue, organ, or organism, and thus the introduced polynucleotide molecule is inherited by subsequent offspring. "Transgenic" or "transformed" cells or organisms also include the offspring of the cells or organisms, as well as offspring generated by breeding procedures that use such transgenic organisms as maternal hybrids and exhibit altered phenotypes caused by the presence of foreign polynucleotide molecules. The term "transgenic" refers to bacteria, fungi, or plants containing one or more heterologous polynucleotide molecules.

[0082] There are many methods for introducing polynucleotide molecules into plant cells. These methods generally involve selecting suitable host cells, transforming the host cells using a recombinant vector, and obtaining the transformed host cells. Suitable methods include bacterial infection (e.g., Agrobacterium spp.), binary bacterial artificial chromosome vectors, direct delivery of DNA (e.g., via PEG-mediated transformation), drying / inhibition-mediated DNA uptake, electroporation, stirring with silicon carbide fibers, and acceleration using DNA-coated particles (reviewed in Potrykus et al., Ann. Rev. Plant Physiol. Plant Mol. Biol., 42: 205 (1991)).

[0083] Any transformation method can be used to transform host cells with one or more promoters and / or constructs of the present invention. The host cell can be any cell or organism such as plant cells, algal cells, fungal cells, bacterial cells, or insect cells. Preferred hosts and transformed cells include cells derived from plants, Aspergillus, yeast, insects, bacteria, and algae.

[0084] Regenerated transgenic plants can self-pollinate to provide homozygous transgenic plants. Optionally, pollen obtained from regenerated transgenic plants can be hybridized with inbred lines of non-transgenic plants, preferably agronomically important species. Descriptions of breeding methods commonly used for different traits and crops can be found in one of several reference books, see, for example, Allard, Principles of Plant Breeding, John Wiley & Sons, NY, U. of CA, Davis, CA, 50-98 (1960); Simmonds, Principles of Crop Improvement, Longman, Inc., NY, 369-399 (1979); Sneep and Hendriksen, Plant Breeding Perspectives, Wageningen (ed), Center for Agricultural Publishing and Documentation (1979); Fehr, Soybeans: Improvement, Production and Uses, 2nd Edition, Monograph, 16: 249 (1987); Fehr, Principles of Variety Development, Theory and Technique, (Vol. 1) and Crop Species Soybean (Vol. 2), Iowa State University, Macmillan Pub.Co., NY, 360-376 (1987). Conversely, pollen from non-GMO plants can be used to pollinate regenerated GMO plants.

[0085] The presence of target genes in transformed plants and the expression levels and / or distribution conferred by the regulatory elements of this invention can be analyzed. Those skilled in the art are aware of many methods that can be used to analyze transformed plants. For example, plant analysis methods include, but are not limited to, Southern blotting or northern blotting, PCR-based methods, biochemical analysis, phenotypic screening, field assessment, and immunodiagnostic assays. The expression of transcribed polynucleotide molecules can be used... (AppliedBiosystems, Foster City, CA) Reagents and manufacturer's description of methods and uses The PCR cycle time is measured using TestingMatrix. Alternatively, (Third Wave Technologies, Madison, WI) reagents and manufacturer-described methods can be used for transgenic expression.

[0086] The seeds of the plants of the present invention can be harvested from fertile transgenic plants and used to grow the plant offspring transformed by the present invention, including plant hybrids that contain the constructs of the present invention and express agronomic target genes.

[0087] This invention also provides parts of the plant described in this invention. Plant parts include, but are not limited to, leaves, stems, roots, tubers, seeds, endosperm, ovules, and pollen. This invention also includes and provides plant cells transformed with nucleic acid molecules comprising the nucleic acid molecules of this invention.

[0088] Transgenic plants can transfer transgenic polynucleotide molecules to their offspring. Offspring include any regenerable plant parts or seeds containing transgenes derived from the ancestral plant. For the transformed polynucleotide molecule, the transgenic plant is preferably homozygous and, as a result of sexual reproduction, transfers the sequence to all offspring. Offspring can grow from seeds generated from the transgenic plant. These additional plants can then self-pollinate to generate true breeding lines of the plant. Among other things, gene expression in the offspring from these plants is evaluated. Gene expression can be detected using several conventional methods such as Western blotting, Northern blotting, immunoprecipitation, and ELISA.

[0089] The invention has now been generally described, and will be more readily understood by referring to the embodiments provided below by way of example, which, unless otherwise specified, are not intended to limit the invention. Those skilled in the art will understand that the techniques disclosed in the following embodiments represent techniques discovered by the inventors that are well-suited for implementing the invention. However, those skilled in the art will understand that many changes can be made to the specific embodiments disclosed in accordance with the invention, and similar or related results can still be obtained without departing from the spirit and scope of the invention; therefore, all content set forth or shown in the accompanying drawings should be understood as exemplary, not restrictive. Detailed Implementation

[0090] Example 1: Identification and Cloning of Control Elements

[0091] Novel transcriptional regulatory elements or transcriptional expression element set (EXP) sequences were identified and isolated from the genomic DNA of the dicotyledonous melon species WSH-39-1070AN.

[0092] Transcriptional regulatory elements were selected based on proprietary and public microarray data derived from transcriptional profiling experiments in soybean (Glycine max) and Arabidopsis thaliana, as well as homology-based searches using known dicotyledonous plant sequences against proprietary melon sequences.

[0093] Using the identified sequences, bioinformatics analysis was performed to identify regulatory elements in the amplified DNA, followed by identification of transcription start sites (TSS) and any bidirectional, intronic, or upstream coding sequences present in the sequence. Using the analysis results, regulatory elements were identified within the DNA sequence, and primers were designed to amplify these elements. The corresponding DNA molecules for each regulatory element were amplified using standard polymerase chain reaction conditions, primers containing unique restriction enzyme sites, and genomic DNA isolated from melon. The resulting DNA fragments were ligated into a basic plant expression vector using standard restriction enzyme digestion and DNA ligation methods compatible with the restriction sites.

[0094] The TSS and intron / exon splicing sites of the regulatory elements can be analyzed using transformed plant protoplasts. In short, the protoplasts are transformed with the plant expression vector containing a cloning DNA fragment operatively linked to a heterologous transcribed polynucleotide molecule, and the TSS and intron / exon splicing sites of the resulting mRNA transcript are confirmed using the 5′RACE system, version 2.0 (Invtrogen, Carlsbad, California 92008), for rapid cDNA end amplification.

[0095] As described above, the sequences of the regulatory expression elements (EXPs) encoding ubiquitin 1 transcription were analyzed, and each EXP was decomposed into its corresponding promoter, leader sequence, and intron. The sequences of the identified EXPs are provided herein under SEQ ID Nos: 1, 5, 7, 9, and 11, and are listed in Table 1 below. The corresponding ubiquitin 1 promoters are provided herein under SEQ ID Nos: 2, 6, 8, 10, and 12. The ubiquitin 1 leader sequences and introns are provided herein under SEQ ID Nos: 3 and 4, respectively.

[0096] SEQ ID Nos: 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 7 4, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 12 7, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 159, 162, 167, 168, 172, 175, 176, 177, 178, 181, 182, 183, 184, 185, 188, 189, 190, 1 Sequences 91, 192, 193, 194, 195, 196, 197, 198, 199, 211, and 212 provide sequences encoding regulatory expression element sets or EXP sequences for transcription of other *Cucumis* species, and these sequences are also listed in Table 1 below. The EXP sequences consist of promoter elements operatively linked to a leader sequence element; or promoter elements operatively linked to a leader sequence element and an intron element; or promoter elements operatively linked to a leader sequence element, operatively linked to an intron element, and operatively linked to a leader sequence element. Additional promoter elements are provided with SEQ ID Nos. 163 and 169. Additional leader sequence elements are provided with SEQ ID Nos. 164, 166, and 170. Additional intron elements are provided with SEQ ID Nos. 165 and 171. Elements in which the promoter is operatively linked to a leader sequence element are provided with SEQ ID Nos. 157, 160, 173, 179, and 186. Elements wherein introns operatively connect leader sequence elements are provided in SEQ ID Nos: 158, 161, 174, 180 and 187.These sequences were selected and cloned based on experimental results, such as promoter-driven transcript distribution or expression from homologous genes from different species, based on the subset of sequences provided with SEQ ID Nos: 13-199, 211, and 212, according to desired expression patterns such as constitutive expression, root expression, above-ground expression, or seed expression. The actual activity conferred by the *Calamus* genus sequences is determined empirically and is not necessarily the same as the activity of regulatory elements derived from homologous genes from species other than *Calamus* when used for transformation of plant host cells and whole transgenic plants.

[0097]

[0098]

[0099]

[0100]

[0101]

[0102]

[0103]

[0104]

[0105]

[0106]

[0107]

[0108]

[0109]

[0110]

[0111]

[0112]

[0113] As shown in Table 1, for example, the transcriptional regulatory expression element set (EXP) represented by EXP-CUCme.Ubq1:1:1 (SEQ ID NO: 1), which has components isolated from melon, includes a promoter element P-CUCme.Ubq1-1:1:15 (SEQ ID NO: 2) with a size (bp) of 2068 base pairs, operably linked to the leader sequence element L-CUCme.Ubq1-1:1:1 (SEQ ID NO: 3) and operably linked to the intron element I-CUCme.Ubq1-1:1:1 (SEQ ID NO: 4). The transcriptional regulatory expression element set (EXP) represented by EXP-CUCme.Ubq1:1:2 (SEQ ID NO: 5), which contains components isolated from melon, comprises a 1459 bp promoter element P-CUCme.Ubq1-1:1:16 (SEQ ID NO: 6) operably linked to the leader sequence element L-CUCme.Ubq1-1:1:1 (SEQ ID NO: 3) and an intron element I-CUCme.Ubq1-1:1:1 (SEQ ID NO: 4). The transcriptional regulatory expression element set (EXP) represented by EXP-CUCme.Ubq1:1:3 (SEQ ID NO: 7), which contains components isolated from melon, comprises a 964 bp promoter element P-CUCme.Ubq1-1:1:17 (SEQ ID NO: 8) operably linked to the leader sequence element L-CUCme.Ubq1-1:1:1 (SEQ ID NO: 3) with 5′ linked to the intron element I-CUCme.Ubq1-1:1:1 (SEQ ID NO: 4). The transcriptional regulatory expression element set (EXP) represented by EXP-CUCme.Ubq1:1:4 (SEQ ID NO: 9), which contains components isolated from melon, comprises a 479 bp promoter element P-CUCme.Ubq1-1:1:18 (SEQ ID NO: 10) that is operatively linked to the leader sequence element L-CUCme.Ubq1-1:1:1 (SEQ ID NO: 3) with a 5′ linker to the intron element I-CUCme.Ubq1-1:1:1 (SEQ ID NO: 4).The transcriptional regulatory expression element set (EXP) represented by EXP-CUCme.Ubq1:1:5 (SEQ ID NO: 11), which contains components isolated from melon, comprises a 173 bp promoter element P-CUCme.Ubq1-1:1:19 (SEQ ID NO: 12) that is operatively linked to the leader sequence element L-CUCme.Ubq1-1:1:1 (SEQ ID NO: 3) with a 5′ linker to the intron element I-CUCme.Ubq1-1:1:1 (SEQ ID NO: 4).

[0114] The comparison of the ubiquitin-1 promoter sequence provides for Figures 1a-1f In addition, promoter elements P-CUCme.Ubq1-1:1:16 (SEQ ID NO: 6), P-CUCme.Ubq1-1:1:17 (SEQ ID NO: 8), P-CUCme.Ubq1-1:1:18 (SEQ ID NO: 10), and P-CUCme.Ubq1-1:1:19 (SEQ ID NO: 12) were constructed by introducing different lengths of missing 5′ ends from the promoter P-CUCme.Ubq1-1:1:15 (SEQ ID NO: 2).

[0115] Example 2: Analysis of the regulatory elements driving GUS in soybean cotyledon protoplasts

[0116] Soybean cotyledonary protoplasts were transformed with a plant expression vector containing a set of test transcriptional regulatory expression elements that drive the expression of the β-glucuronidase (GUS) transgene, and compared with GUS expression in leaf protoplasts, wherein the expression of GUS is driven by a known constitutive promoter.

[0117] Transgenic expression driven by EXP-CUCme.Ubq1:1:1 (SEQ ID NO: 1), EXP-CUCme.Ubq1:1:2 (SEQ ID NO: 5), EXP-CUCme.Ubq1:1:3 (SEQ ID NO: 7), EXP-CUCme.Ubq1:1:4 (SEQ ID NO: 9), and EXP-CUCme.Ubq1:1:5 (SEQ ID NO: 11) was compared with expression from known constitutive promoters. Each plant expression vector consists of a right boundary region from *Agrobacterium tumefaciens*, a first transgenic cassette, a second transgenic selection cassette, and a left boundary region from *Agrobacterium tumefaciens*. The first transgenic cassette comprises a 5′ region operably linked to a coding sequence of β-glucuronidase (GUS, SEQ ID NO: 206) containing a processable intron derived from the potato light-induced tissue-specific ST-LS1 gene (gene bank accession number: X04753), and a 5′ region operably linked to the coding sequence of the following genes: *Gossypium barbadense* E6 gene (T-Gb.E6-3b:1:1, SEQ ID NO: 204), *Pisumsativum* RbcS2-E9 gene (T-Ps.RbcS2-E9-1:1:6, SEQ ID NO: 203), or *Gossypium barbadense* FbLate-2 gene (T-Gb.FbL2-1:1:1, SEQ ID NO: 204). The EXP sequence of the 3′ termination region of NO: 205 or a known constitutive promoter composition; the second transgenic selection cassette is used to select plant cells conferred resistance to the herbicide glyphosate (driven by the Arabidopsis actin 7 promoter) or the antibiotic kanamycin. A promoterless control plant expression vector (pMON124912) is used as a negative control for expression. The above-tested and constitutive expression element sets are cloned into plant expression vectors as shown in Table 2 below.

[0118] Table 2. Plant expression vectors and corresponding expression element sets and 3′UTR

[0119]

[0120] expression carrier Control element SEQ ID NO: 3′UTR pMON124912 No promoter T-Gb.FbL2-1:1:1 pMON138776 EXP-CUCme.Ubq1:1:1 1 T-Gb.FbL2-1:1:1 pMON138777 EXP-CUCme.Ubq1:1:2 5 T-Gb.FbL2-1:1:1 pMON138778 EXP-CUCme.Ubq1:1:3 7 T-Gb.FbL2-1:1:1 pMON138779 EXP-CUCme.Ubq1:1:4 9 T-Gb.FbL2-1:1:1 pMON138780 EXP-CUCme.Ubq1:1:5 11 T-Gb.FbL2-1:1:1

[0121] In addition, two plasmids were constructed for co-transformation and data normalization. One transformation control plasmid consists of a constitutive promoter that drives the expression of the *Photinus pyralis* luciferase-coding sequence (FLuc, SEQ ID NO: 207), the constitutive promoter being operatively linked 5′ to the 3′ termination region of the *Agrobacterium tumefaciens* carmine synthase gene (T-AGRtu.nos-1:1:13, SEQ ID NO: 209). The other transformation control plasmid consists of a constitutive promoter that drives the expression of the *Renilla reniformis* luciferase-coding sequence (RLuc, SEQ ID NO: 208), the constitutive promoter being operatively linked 5′ to the 3′ termination region of the *Agrobacterium tumefaciens* carmine synthase gene.

[0122] Soybean cotyledon protoplast cells were transformed using the PEG transformation method with plant expression vectors pMON80585, pMON109584, pMON118756, pMON124912, pMON138776, pMON138777, pMON138778, pMON138779, and pMON138780. Equimolar amounts of each of the two transformation control plasmids and the test plant expression vector were used to transform protoplast cells. GUS and luciferase activities were measured. GUS and luciferase were measured by placing aliquots of the lysate of the transformed cells into two separate well trays. One tray was used for GUS measurement, while the other was used for dual-luciferase detection using a dual-luciferase reporter gene detection system (Promega Corp., Madison, WI; see, for example, Promega Notes Magazine, No: 57, 1996, p. 02). Three or four replicates were used per transformation for sample measurement. The average values ​​of GUS and luciferase are shown in Table 3 below.

[0123] Table 3. Mean expression levels of GUS and luciferase, and GUS / luciferase ratio

[0124]

[0125]

[0126] To compare the relative activity of each promoter in soybean cotyledon protoplasts, GUS values ​​were expressed as the ratio of GUS to luciferase activity and normalized according to the observed expression levels of the constitutive expression element sets EXP-At.Act7:1:11 and EXP-CaMV.35S-enh+Ph.DnaK:1:3. Table 4 below shows the ratio of GUS to firefly luciferase (FLuc) normalized according to EXP-At.Act7:1:11 and EXP-CaMV.35S-enh+Ph.DnaK:1:3. Table 5 below shows the ratio of GUS to Renilla luciferase (RLuc) normalized according to EXP-At.Act7:1:11 and EXP-CaMV.35S-enh+Ph.DnaK:1:3.

[0127] Table 4. Ratios of GUS to firefly luciferase (FLuc) normalized according to EXP-At.Act7:1:11 and EXP-CaMV.35S-enh+Ph.DnaK:1:3

[0128]

[0129] Table 5. Ratios of GUS to Renal luciferase (RLuc) normalized according to EXP-At.Act7:1:11 and EXP-CaMV.35S-enh+Ph.DnaK:1:3

[0130]

[0131]

[0132] As shown in Tables 4 and 5 above, each of the expression element groups EXP-CUCme.Ubq1:1:1 (SEQ ID NO: 1), EXP-CUCme.Ubq1:1:2 (SEQ ID NO: 5), EXP-CUCme.Ubq1:1:3 (SEQ ID NO: 7), EXP-CUCme.Ubq1:1:4 (SEQ ID NO: 9), and EXP-CUCme.Ubq1:1:5 (SEQ ID NO: 11) demonstrates the ability to drive transgene expression in soybean cotyledon protoplasts. Their expression levels in this assay were higher than those of EXP-At.Act7:1:11, and were 2.9 to 5.8 times (FLuc) or 3 to 7 times (RLuc) higher than those of EXP-At.Act7:1:11. The expression was comparable to or higher than the observed expression of EXP-CaMV.35S-enh+Ph.DnaK:1:3. The expression level was 0.8 to 1.7 times (FLuc) or 1 to 2.4 times (RLuc) higher than the observed expression of EXP-CaMV.35S-enh+Ph.DnaK:1:3.

[0133] Example 3: Analysis of the regulatory elements driving GUS in bombarded soybean leaves and roots

[0134] Soybean leaves and roots were transformed with a plant expression vector containing a set of test transcriptional regulatory expression elements that drive the expression of the β-glucuronidase (GUS) transgene, and compared with GUS expression in roots and leaves, wherein the expression of GUS is driven by a known constitutive promoter.

[0135] Transgenic expression driven by EXP-CUCme.Ubq1:1:1 (SEQ ID NO: 1), EXP-CUCme.Ubq1:1:2 (SEQ ID NO: 5), EXP-CUCme.Ubq1:1:3 (SEQ ID NO: 7), EXP-CUCme.Ubq1:1:4 (SEQ ID NO: 9), and EXP-CUCme.Ubq1:1:5 (SEQ ID NO: 11) in soybean leaves and roots bombarded with particles was compared with expression from known constitutive promoters. The plant expression vectors used for transforming leaves and roots were the same as those shown in Table 2 of Example 2 above.

[0136] The plant expression vectors pMON80585, pMON109584, pMON118756, pMON124912, pMON138776, pMON138777, pMON138778, pMON138779, and pMON138780 were used to transform soybean leaves and roots using the particle bombardment transformation method.

[0137] In short, A3244 soybean seeds were surface-sterilized and germinated in trays using a photocycle of 16 hours light and 8 hours dark. After approximately 13 days, leaf and root tissues were harvested from the seedlings under aseptic conditions and used for bombardment. The tissue samples were randomly placed on Petri dishes containing plant culture medium. 0.6-micron gold particles (catalog #165-2262 Bio-Rad, Hercules, CA) were used for bombardment with 10 μg of plasmid DNA. Large vectors were loaded onto the DNA-coated gold particles (catalog #165-2335 Bio-Rad, Hercules CA). Transformation was performed using a PDS 1000 / He gene gun (catalog #165-2257 Bio-Rad, Hercules CA). The bombarded root and leaf tissues were cultured in the dark at 26°C for 24 hours. Following overnight culture, the tissues were stained overnight at 37°C in a solution for GUS expression. After overnight staining, the tissues were soaked overnight in 70% ethanol to remove chlorophyll and reveal GUS staining. The tissues were then photographed, and each construct was assigned a gradation scale reflecting GUS expression levels of “0”, “+” to “++++++” (0 for no expression, + to ++++++ for low to high).

[0138] The expression of GUS transgenes shown in each tissue was used to infer the relative potential level and specificity of each element's ability to drive transgene expression in stably transformed maize plants. The average gradation of GUS expression is provided in Table 6 below.

[0139] Table 6. GUS expression levels in leaves and roots after particle bombardment

[0140]

[0141]

[0142] As can be seen from Table 6 above, each of the expression element groups EXP-CUCme.Ubq1:1:1 (SEQ ID NO: 1), EXP-CUCme.Ubq1:1:2 (SEQ ID NO: 5), EXP-CUCme.Ubq1:1:3 (SEQ ID NO: 7), EXP-CUCme.Ubq1:1:4 (SEQ ID NO: 9), and EXP-CUCme.Ubq1:1:5 (SEQ ID NO: 11) demonstrates the ability to drive transgene expression in transformed leaf and root tissues by particle bombardment.

[0143] Example 4: Analysis of regulatory elements driving GUS in soybean cotyledon protoplasts

[0144] Soybean cotyledonary protoplasts were transformed with a plant expression vector containing a set of test transcriptional regulatory expression elements that drive the expression of the β-glucuronidase (GUS) transgene, and compared with GUS expression in leaf protoplasts, wherein the expression of GUS is driven by a known constitutive promoter.

[0145] It will be composed of P-CUCme.1-1:1:1rc (SEQ ID NO:155), P-CUCme.2-1:1:1 (SEQ ID NO:14), P-CUCme.3-1:1:3 (SEQ ID NO:15), EXP-CUCme.4:1:1 (SEQ ID NO:156), EXP-CUCme.5:1:1 (SEQ ID NO:15) NO:159), P-CUCme.6-1:1:1 (SEQ ID NO:18), P-CUCme.8-1:1:2 (SEQ ID NO:19), P-CUCme.9-1:1:2 (SEQ ID NO:20), P-CUCme.10-1:1:1 (SEQ ID NO:21), EXP-CUCme.eEF1a:1:1 (SEQ ID NO: 162), P-CUCme.15-1: 1: 2 (SEQ ID NO:23), P-CUCme.16a-1:1:2 (SEQ ID NO:24), P-CUCme.17-1:1:2 (SEQ ID NO:26), P-CUCme.18-1:1:2 (SEQ ID NO:27), P-CUCme.19-1:1:3 (SEQ ID NO:167), P-CUCme.20-1:3 (SEQ ID NO:211), P-CUCme.21-1:1:1 (SEQ ID NO:30), P-CUCme.22-1:1:3 (SEQ ID NO:31), EXP-CUCme.SAMS2:1:1 (SEQ ID NO:168), P-CUCme.26-1:1:2 (SEQ ID NO:33), P-CUCme.28-1:1:2 (SEQ ID NO:34) and EXP-CUCme.29:1:2 (SEQ ID The expression of the NO:212-driven transgene was compared with that from known constitutive expression element groups.Each plant expression vector consists of a right boundary region from *Agrobacterium tumefaciens*, a first transgenic cassette, a second transgenic selection cassette, and a left boundary region from *Agrobacterium tumefaciens*. The first transgenic cassette comprises a 5′-linked gene encoding a β-glucuronidase (GUS, SEQ ID NO: 206) containing a processable intron derived from the potato light-induced tissue-specific ST-LS1 gene (gene bank accession number: X04753), and a 5′-linked gene encoding a gene from *Cotton Island cotton* E6 gene (T-Gb.E6-3b:1:1, SEQ ID NO: 204), pea RbcS2-E9 gene (T-Ps.RbcS2-E9-1:1:6, SEQ ID NO: 203), or *Cotton Island cotton* FbLate-2 gene (T-Gb.FbL2-1:1:1, SEQ ID NO: 204). The test promoter or a known constitutive promoter is composed of the 3′ termination region of NO: 205; the second transgenic selection cassette is used to select plant cells conferred resistance to the herbicide glyphosate (driven by the Arabidopsis actin 7 promoter) or the antibiotic kanamycin. A promoterless control plant expression vector (pMON124912) is used as a negative control for expression. The above test and constitutive expression element sets are cloned into plant expression vectors as shown in Table 7 below.

[0146] Table 7. Plant expression vectors and corresponding expression element sets and 3′UTR

[0147]

[0148]

[0149] In addition, two plasmids were constructed for co-transformation and data normalization. One transformation control plasmid consists of a constitutive promoter that drives the expression of the *Photinus pyralis* luciferase-coding sequence (FLuc, SEQ ID NO: 207), the constitutive promoter being operatively linked 5′ to the 3′ termination region of the *Agrobacterium tumefaciens* carmine synthase gene (T-AGRtu.nos-1:1:13, SEQ ID NO: 209). The other transformation control plasmid consists of a constitutive promoter that drives the expression of the *Renilla reniformis* luciferase-coding sequence (RLuc, SEQ ID NO: 208), the constitutive promoter being operatively linked 5′ to the 3′ termination region of the *Agrobacterium tumefaciens* carmine synthase gene.

[0150] The PEG transformation method was used to transform the plant expression vectors pMON80585, pMON109584, pMON118756, pMON124912, pMON140818, pMON140819, pMON140820, pMON140821, pMON140822, pMON140823, pMON140824, pMON140825, and pMON140826. 6. pMON140827, pMON140828, pMON140829, pMON140830, pMON140831, pMON140832, pMON140833, pMON140834, pMON140835, pMON140836, pMON140837, pMON140838, and pMON140839 were used to transform soybean cotyledon protoplast cells. Equimolar amounts of each of the two transformation control plasmids and the test plant expression vector were used to transform protoplast cells. GUS and luciferase activities were measured. GUS and luciferase activities were measured by placing equal aliquots of the lysis formulation of the transformed cells into two separate well trays. One tray was used for GUS measurement, while the other tray was used for dual-luciferase detection using a dual-luciferase reporter gene detection system (Promega Corp., Madison, WI; see, for example, Promega Notes Magazine, No: 57, 1996, p. 02). Three or four replicas were used for sample measurement per transformation. The average values ​​for GUS and luciferase are shown in Table 8 below.

[0151] Table 8. Mean GUS and luciferase expression and GUS / luciferase ratio

[0152]

[0153]

[0154] To compare the relative activity of each promoter in soybean cotyledon protoplasts, GUS values ​​were expressed as the ratio of GUS to luciferase activity and normalized according to the observed expression levels of the constitutive expression element sets EXP-At.Act7:1:11 and EXP-CaMV.35S-enh+Ph.DnaK:1:3. Table 9 below shows the ratio of GUS to firefly luciferase (FLuc) normalized according to EXP-At.Act7:1:11 and EXP-CaMV.35S-enh+Ph.DnaK:1:3. Table 10 below shows the ratio of GUS to Renilla luciferase (RLuc) normalized according to EXP-At.Act7:1:11 and EXP-CaMV.310S-enh+Ph.DnaK:1:3.

[0155] Table 9. Ratios of GUS to firefly luciferase (FLuc) normalized according to EXP-At.Act7:1:11 and EXP-CaMV.35S-enh+Ph.DnaK:1:3

[0156]

[0157]

[0158] Table 10. Ratios of GUS to Renal luciferase (RLuc) normalized according to EXP-At.Act7:1:11 and EXP-CaMV.35S-enh+Ph.DnaK:1:3

[0159]

[0160]

[0161] As can be seen from Tables 9 and 10, most of the tested expression element sets demonstrated the ability to drive transgene expression in soybean cotyledon protoplast cells. In this assay, one expression element set, EXP-CUCme.4:1:1 (SEQ ID NO: 156), showed a higher transgene expression level than EXP-At.Act7:1:11.

[0162] Example 5: Analysis of the regulatory elements driving GUS in bombarded soybean leaves and roots

[0163] Soybean leaves and roots were transformed with a plant expression vector containing a set of test transcriptional regulatory expression elements that drive the expression of the β-glucuronidase (GUS) transgene, and compared with GUS expression in roots and leaves, wherein the expression of GUS is driven by a known constitutive promoter.

[0164] The following peptides will be used in the powder-bombarded soybean leaves and roots: P-CUCme.1-1:1:1rc (SEQ ID NO: 155), P-CUCme.2-1:1:1 (SEQ ID NO: 14), P-CUCme.3-1:1:3 (SEQ ID NO: 15), EXP-CUCme.4:1:1 (SEQ ID NO: 156), EXP-CUCme.5:1:1 (SEQ ID NO: 159), P-CUCme.6-1:1:1 (SEQ ID NO: 18), P-CUCme.8-1:1:2 (SEQ ID NO: 19), P-CUCme.9-1:1:2 (SEQ ID NO: 20), P-CUCme.10-1:1:1 (SEQ ID NO: 21), EXP-CUCme.eEF1a:1:1 (SEQ ID NO: 162), and P-CUCme.15-1:1:2 (SEQ ID NO: 155). NO:23), P-CUCme.16a-1:1:2 (SEQ ID NO:24), P-CUCme.17-1:1:2 (SEQ ID NO:26), P-CUCme.18-1:1:2 (SEQ ID NO:27), P-CUCme.19-1:1:3 (SEQ ID NO:167), P-CUCme.20-1:3 (SEQ ID NO:211), P-CUCme.21-1:1:1 (SEQ ID NO:30), P-CUCme.22-1:1:3 (SEQ ID NO:31), EXP-CUCme.SAMS2:1:1 (SEQ ID NO:168), P-CUCme.26-1:1:2 (SEQ ID NO:33), P-CUCme.28-1:1:2 (SEQ ID NO:34) and EXP-CUCme.29:1:2 (SEQ ID The expression of the transgene driven by NO: 212 was compared with that from known constitutive expression element sets. The plant expression vectors used for transforming leaves and roots were the same as those shown in Table 7 of Example 4 above.

[0165] Particle bombardment transformation was used, employing plant expression vectors pMON80585, pMON109584, pMON118756, pMON124912, pMON140818, pMON140819, pMON140820, pMON140821, pMON140822, pMON140823, pMON140824, pMON140825, and pMON14. 0826, pMON140827, pMON140828, pMON140829, pMON140830, pMON140831, pMON140832, pMON140833, pMON140834, pMON140835, pMON140836, pMON140837, pMON140838 and pMON140839 were used to convert soybean leaves and roots.

[0166] In short, A3244 soybean seeds were surface-sterilized and germinated in trays using a photocycle of 16 hours light and 8 hours dark. After approximately 13 days, leaf and root tissues were harvested from the seedlings under aseptic conditions and used for bombardment. The tissue samples were randomly placed on Petri dishes containing plant culture medium. 0.6-micron gold particles (catalog #165-2262 Bio-Rad, Hercules, CA) were used for bombardment with 10 μg of plasmid DNA. Large vectors were loaded onto the DNA-coated gold particles (catalog #165-2335 Bio-Rad, Hercules CA). Transformation was performed using a PDS 1000 / He gene gun (catalog #165-2257 Bio-Rad, Hercules CA). The bombarded root and leaf tissues were cultured in the dark at 26°C for 24 hours. Following overnight culture, the tissues were stained overnight at 37°C in a solution for GUS expression. After overnight staining, the tissues were soaked overnight in 70% ethanol to remove chlorophyll and reveal GUS staining. The tissues were then photographed, and each construct was assigned a gradation scale reflecting GUS expression levels of “0”, “+” to “++++++” (0 for no expression, + to ++++++ for low to high).

[0167] The expression of GUS transgenes shown in each tissue was used to infer the relative potential level and specificity of each element's ability to drive transgene expression in stably transformed maize plants. The mean gradation of GUS expression is provided in Table 11 below.

[0168] Table 11. GUS expression levels in leaves and roots after particle bombardment

[0169]

[0170] As can be seen from Table 11 above, all but one of the expression element groups showed the ability to drive transgene expression in soybean leaf and root tissues bombarded by particles. In this assay, the two expression element groups P-CUCme.28-1:1:2 (SEQ ID NO: 34) and EXP-CUCme.4:1:1 (SEQ ID NO: 156) showed similar or higher expression levels compared to expression driven by EXP-CaMV.35S-enh+Ph.DnaK:1:3.

[0171] Example 6: Analysis of the regulatory elements driving GUS in soybean cotyledon protoplasts using transgenic cassette amplicon

[0172] Soybean cotyledonary protoplasts were transformed with a transgenic cassette amplicon containing a set of transcriptional regulatory expression elements that drive the expression of the β-glucuronidase (GUS) transgene, and the expression was compared with GUS expression in leaf protoplasts, wherein GUS expression is driven by a known constitutive promoter. The transgenic cassette amplicon consists of an EXP sequence operably linked to a GUS coding sequence (GUS, SEQ ID NO: 206) and an EXP sequence operably linked to a 3′UTR (T-Gb.FbL2-1:1:1, SEQ ID NO: 205). Average GUS expression was compared with the control EXP elements P-CaMV.35S-enh-1:1:102 / L-CaMV.35S-1:1:2 (SEQ ID NO: 210) and EXP-At.Atntt1:1:2 (SEQ ID NO: 200).

[0173] Furthermore, plasmids for co-transformation and data normalization were used in a similar manner to that described above in Example 2. The transformation control plasmid consisted of a constitutive promoter that drives the expression of a firefly (Photinus pyralis) luciferase-coding sequence (FLuc, SEQ ID NO: 205), which was operatively linked at 5′ to the 3′ termination region of the Agrobacterium tumefaciens carmine synthase gene (T-AGRtu.nos-1:1:13, SEQ ID NO: 209).

[0174] Table 12 below shows the average GUS expression conferred by each transgenic amplicon. Table 13 below shows the ratio of GUS to firefly luciferase (FLuc) normalized according to EXP-At.Atntt1:1:2 and P-CaMV.35S-enh-1:1:102 / L-CaMV.35S-1:1:2.

[0175]

[0176]

[0177]

[0178]

[0179] As can be seen from Table 12 above, not all EXP sequences showed the ability to drive transgene expression when compared with the promoter-free control. However, the EXP sequences CumMe_WSM_SF16429.G5670 (SEQ ID NO: 40), P-CUCme.CumMe_WSM_SF16444.G5140-1: 1: 1 (SEQ ID NO: 175), P-CUCme.CumMe_WSM_SF_16563.G5560-1: 1: 1 (SEQ ID NO: 176), CumMe_WSM_SF17051.G5470 (SEQ ID NO: 48), P-CUCme.CumMe_WSM_SF17111.G5790-1: 1: 1 (SEQ ID NO: 177), P-CUCme.WSM_SF17252.G7330-1: 1: 1 (SEQ ID NO: 179), CumMe_WSM_SF17866.G6050 (SEQ ID NO: 62), P-CUCme.CumMe_WSM_SF18488.G5340-1: 1: 1 (SEQ ID NO: 181), P-CUCme.CumMe_WSM_SF_18536.G6480-1: 1: 1 (SEQ ID NO: 182), CumMe_WSM_SF18575.G6410 (SEQ ID NO:71), P-CUCme.CumMe_WSM_SF_18634.G5190-1:1:1 (SEQ ID NO:183), CumMe_WSM_SF18986.G6110 (SEQ ID NO:79), EXP-CUCme.WSM_SF_19064.G5690:1:1 (SEQ ID NO: 185), P-CUCme.CumMe_WSM_SF_19647.G5760-1: 1: 1 (SEQ ID NO: 188), P-CUCme.CumMe_WSM_SF19839.G5090-1: 1: 1 (SEQ ID NO: 189), CumMe_WSM_SF19902.G5260 (SEQ ID NO: 87), P-CUCme.CumMe_WSM_SF20132.G5560-1:1:1 (SEQ ID NO: 190), CumMe_WSM_SF20359.G5870 (SEQ ID NO: 92), CumMe_WSM_SF206458.G5970 (SEQ ID NO: 98), CumMe_WSM_SF206534.G5200 (SEQ ID NO: 99), CumMe_WSM_SF22008.G5670 (SEQ ID NO: 108), CumMe_WSM_SF22355.G5310 (SEQ ID NO: 113), P-CUCme.CumMe_WSM_SF22531.G5120-1: 1: 1 (SEQ ID NO: 192), EXP-CUCme.WSM_SF19064.G5690: 1: 1 (SEQ ID NO: 193), P-CUCme.CumMe_WSM_SF23906.G6180-1: 1: 1 (SEQ ID NO: 194), CumMe_WSM_SF24045.G5400 (SEQ ID NO: 123), P-CUCme.CumMe_WSM_SF25141.G5160-1: 1: 2 (SEQ ID P-CUCme.CumMe_WSM_SF25936.G5450-1:1:1 (SEQ ID NO: 197), CumMe_WSM_SF28729.G5340 (SEQ ID NO: 134), CumMe_WSM_SF31264.G5380 (SEQ ID NO: 136), and P-CUCme.CumMe_WSM_SF35856.G5150-1:1:1 (SEQ ID NO: 198) showed the ability to drive transgene expression in soybean cotyledon protoplasts at levels similar to or higher than EXP-At.Atntt1:1:2. As shown in Table 13 above, in this assay, the EXP sequence P-CUCme.CumMe_WSM_SF19647.G5760-1:1:1 (SEQ ID NO: 188) demonstrated an ability to drive transgene expression at a higher level than EXP-At.Atntt1:1:2.

[0180] Example 7: Analysis of the regulatory elements driving GUS in cotton leaf protoplasts

[0181] Cotton leaf protoplasts were transformed with a plant expression vector containing a set of test transcriptional regulatory expression elements that drive the expression of the β-glucuronidase (GUS) transgene, and compared with GUS expression in leaf protoplasts, wherein GUS expression is driven by a known constitutive promoter.

[0182] It will be composed of P-CUCme.1-1:1:1rc (SEQ ID NO:155), P-CUCme.2-1:1:1 (SEQ ID NO:14), P-CUCme.3-1:1:3 (SEQ ID NO:15), EXP-CUCme.4:1:1 (SEQ ID NO:156), P-CUCme.6-1:1:1 (SEQ ID NO:15) NO:18), P-CUCme.8-1:1:2 (SEQ ID NO:19), P-CUCme.9-1:1:2 (SEQ ID NO:20), P-CUCme.10-1:1:1 (SEQ ID NO:21), EXP-CUCme.eEF1a:1:1 (SEQ ID NO:162), P-CUCme.15-1:1:2 (SEQ ID NO:23), P-CUCme.16a-1:1:2 (SEQ ID Transgenic expression driven by P-CUCme.17-1:1:2 (SEQ ID NO: 26), P-CUCme.18-1:1:2 (SEQ ID NO: 27), P-CUCme.19-1:1:3 (SEQ ID NO: 167), P-CUCme.20-1:3 (SEQ ID NO: 211), P-CUCme.21-1:1:1 (SEQ ID NO: 30), P-CUCme.22-1:1:3 (SEQ ID NO: 31), EXP-CUCme.SAMS2:1:1 (SEQ ID NO: 168), P-CUCme.26-1:1:2 (SEQ ID NO: 33), P-CUCme.28-1:1:2 (SEQ ID NO: 34), and EXP-CUCme.29:1:2 (SEQ ID NO: 212) was compared with expression from known constitutive expression element groups.Each plant expression vector consists of a right boundary region from *Agrobacterium tumefaciens*, a first transgenic cassette, a second transgenic selection cassette, and a left boundary region from *Agrobacterium tumefaciens*. The first transgenic cassette comprises a 5′-linked gene encoding a β-glucuronidase (GUS, SEQ ID NO: 206) containing a processable intron derived from the potato light-induced tissue-specific ST-LS1 gene (gene bank accession number: X04753), and a 5′-linked gene encoding a gene from *Cotton Island cotton* E6 gene (T-Gb.E6-3b:1:1, SEQ ID NO: 204), pea RbcS2-E9 gene (T-Ps.RbcS2-E9-1:1:6, SEQ ID NO: 203), or *Cotton Island cotton* FbLate-2 gene (T-Gb.FbL2-1:1:1, SEQ ID NO: 204). The test promoter or a known constitutive promoter is composed of the 3′ termination region of NO: 205; the second transgenic selection cassette is used to select plant cells conferred resistance to the herbicide glyphosate (driven by the Arabidopsis actin 7 promoter) or the antibiotic kanamycin. A promoterless control plant expression vector (pMON124912) is used as a negative control for expression. The above test and constitutive expression element sets are cloned into plant expression vectors as shown in Table 14 below.

[0183] Table 14. Plant expression vectors and corresponding expression element sets and 3′UTR

[0184]

[0185] In addition, two plasmids were constructed for co-transformation and data normalization. One transformation control plasmid consists of a constitutive promoter that drives the expression of the *Photinus pyralis* luciferase-coding sequence (FLuc, SEQ ID NO: 205), said constitutive promoter being operatively linked 5′ to the 3′ termination region of the *Agrobacterium tumefaciens* carmine synthase gene (T-AGRtu.nos-1:1:13, SEQ ID NO: 209). The other transformation control plasmid contains a constitutive promoter that drives the expression of the *Renilla reniformis* luciferase-coding sequence (RLuc, SEQ ID NO: 206), said constitutive promoter being operatively linked 5′ to the 3′ termination region of the *Agrobacterium tumefaciens* carmine synthase gene.

[0186] The PEG transformation method was used to transform the plant expression vectors pMON80585, pMON109584, pMON118756, pMON124912, pMON140818, pMON140819, pMON140820, pMON140821, pMON140822, pMON140823, pMON140824, pMON140825, and pMON1408. 26. pMON140827, pMON140828, pMON140829, pMON140830, pMON140831, pMON140832, pMON140833, pMON140834, pMON140835, pMON140836, pMON140837, pMON140838, and pMON140839 were used to transform cotton leaf protoplast cells. Equimolar amounts of each of the two transformation control plasmids and the test plant expression vector were used to transform protoplast cells. GUS and luciferase activities were measured. GUS and luciferase activities were measured by placing equal aliquots of the lysis formulation of the transformed cells as described above into two separate well trays. One tray was used for GUS measurement, while the other tray was used for dual-luciferase detection using a dual-luciferase reporter gene detection system (PromegaCorp., Madison, WI; see, for example, Promega Notes Magazine, No: 57, 1996, p. 02). Four replicates were used for sample measurement per transformation. The average values ​​for GUS and luciferase are shown in Table 15 below.

[0187] Table 15. Mean GUS and luciferase expression and GUS / luciferase ratio

[0188]

[0189] To compare the relative activity of each promoter in cotton leaf protoplasts, GUS values ​​were expressed as the ratio of GUS to luciferase activity and normalized according to the observed expression levels of the constitutive expression element sets EXP-At.Act7:1:11 and EXP-CaMV.35S-enh+Ph.DnaK:1:3. Table 16 below shows the ratio of GUS to firefly luciferase (FLuc) normalized according to EXP-At.Act7:1:11 and EXP-CaMV.35S-enh+Ph.DnaK:1:3. Table 17 below shows the ratio of GUS to Renilla luciferase (RLuc) normalized according to EXP-At.Act7:1:11 and EXP-CaMV.317S-enh+Ph.DnaK:1:3.

[0190] Table 16. Ratios of GUS to firefly luciferase (FLuc) normalized according to EXP-At.Act7:1:11 and EXP-CaMV.35S-enh+Ph.DnaK:1:3

[0191]

[0192] Table 17. Ratios of GUS to Renal luciferase (RLuc) normalized according to EXP-At.Act7:1:11 and EXP-CaMV.35S-enh+Ph.DnaK:1:3

[0193]

[0194] As can be seen from Tables 16 and 17, most of the tested expression element sets demonstrated the ability to drive transgene expression in cotton leaf protoplast cells. In this assay, one expression element set, EXP-CUCme.4:1:1 (SEQ ID NO: 156), showed a higher transgene expression level than EXP-At.Act7:1:11.

[0195] Example 8: Analysis of the regulatory elements driving GUS in cotton leaf protoplasts using transgenic cassette amplicon

[0196] Cotton leaf protoplasts were transformed with a transgenic cassette amplicon containing a set of transcriptional regulatory expression elements that drive the expression of the β-glucuronidase (GUS) transgene, and the expression was compared with GUS expression in leaf protoplasts, wherein GUS expression is driven by a known constitutive promoter. The transgenic cassette amplicon consists of an EXP sequence operably linked to a GUS coding sequence (GUS, SEQ ID NO: 206) and operably linked to a 3′UTR (T-Gb.FbL2-1:1:1, SEQ ID NO: 205). Average GUS expression was compared with the control EXP elements P-CaMV.35S-enh-1:1:102 / L-CaMV.35S-1:1:2 (SEQ ID NO: 210) and EXP-At.Atntt1:1:2 (SEQ ID NO: 200).

[0197] Furthermore, plasmids for co-transformation and data normalization were used in a similar manner to that described above in Example 2. The transformation control plasmid consisted of a constitutive promoter that drives the expression of a firefly (Photinus pyralis) luciferase-coding sequence (FLuc, SEQ ID NO: 205), which was operatively linked at 5′ to the 3′ termination region of the Agrobacterium tumefaciens carmine synthase gene (T-AGRtu.nos-1:1:13, SEQ ID NO: 209).

[0198] Table 18 below shows the average GUS expression conferred by each transgenic amplicon. Table 19 below shows the ratio of GUS to firefly luciferase (FLuc) normalized according to EXP-At.Atnttl:1:2 and P-CaMV.35S-enh-1:1:102 / L-CaMV.35S-1:1:2.

[0199] Table 18. Mean GUS and luciferase expression and GUS / luciferase ratio

[0200]

[0201]

[0202] Table 19. Ratio of GUS to firefly luciferase (FLuc) normalized according to EXP-At.Atntt1:1:2 and P-CaMV.35S-enh-1:1:102 / L-CaMV.35S-1:1:2

[0203]

[0204]

[0205] As can be seen from Table 18 above, not all EXP sequences showed the ability to drive transgene expression when compared with the promoter-free control. However, the EXP sequences P-CUCme.CumMe_WSM_SF16444.G5140-1:1:1 (SEQ ID NO: 175) and P-CUCme.CumMe_WSM_SF19839.G5090-1:1:1 (SEQ ID NO: 189) showed the ability to drive transgene expression in soybean cotyledon protoplasts at levels similar to or higher than EXP-At.Atntt1:1:2. As shown in Table 19 above, in this assay, the EXP sequence P-CUCme.CumMe_WSM_SF19839.G5090-1:1:1 (SEQ ID NO: 189) showed the ability to drive transgene expression at a higher level than EXP-At.Atntt1:1:2.

[0206] Example 9: Analysis of the regulatory elements driving GUS in stable-converting soybean

[0207] Soybean plants were transformed with a plant expression vector containing an EXP sequence that drives the expression of the β-glucuronidase (GUS) transgene.

[0208] The presence of EXP-CUCme.Ubq1:1:1 (SEQ ID NO: 1), EXP-CUCme.Ubq1:1:3 (SEQ ID NO: 7), P-CUCme.1-1:1:1rc (SEQ ID NO: 155), P-CUCme.2-1:1:1 (SEQ ID NO: 14), P-CUCme.3-1:1:3 (SEQ ID NO: 15), EXP-CUCme.4:1:1 (SEQ ID NO: 156), EXP-CUCme.5:1:1 (SEQ ID NO: 159), P-CUCme.6-1:1:1 (SEQ ID NO: 18), P-CUCme.8-1:1:2 (SEQ ID NO: 19), P-CUCme.9-1:1:2 (SEQ ID NO: 20), and P-CUCme.10-1:1:1 (SEQ ID NO: 155) was qualitatively and quantitatively detected by examining stained tissue sections. ID NO:21), EXP-CUCme.eEF 1a:1:1 (SEQ ID NO:162), P-CUCme.15-1:1:2 (SEQ ID NO:23), P-CUCme.17-1:1:2 (SEQ ID NO:26), P-CUCme.18-1:1:2 (SEQ ID NO:27), P-CUCme.19-1:1:3 (SEQ ID NO:167), P-CUCme.20-1:3 (SEQ ID NO:211), P-CUCme.21-1:1:1 (SEQ ID NO:30), EXP-CUCme.SAMS2:1:1 (SEQ ID NO: 168), P-CUCme.26-1: 1: 2 (SEQ ID NO: 33), EXP-CUCme. 29: 1: 2 (SEQ ID NO: 212), P-CUCme.CumMe_WSM_SF25355.G5000-1: 1: 1 (SEQ ID NO: 196), P-CUCme.CumMe_WSM_SF17111.G5790-1: 1: 1 (SEQ ID NO: 177), P-CUCme.CumMe_WSM_SF22531.G5120-1: 1: 1 (SEQ ID NO: 192), P-CUCme.CumMe_WSM_SF18488.G5340-1: 1: 1 (SEQ ID NO: 181), P-CUCme.CumMe_WSM_SF23760.G5200-1: 1: 1 (SEQ ID NO: 193), EXP-CUCme.WSM_SF19064.G5690: 1: 1 (SEQ ID NO: 185), P-CUCme.WSM_SF17252.G7330-1:1:1 (SEQ ID NO:179), P-CUCme.CumMe_WSM_SF18634.G5190-1:1:1 (SEQID NO:183), P-CUCme.CumMe_WSM_SF19647.G5760-1:1:1 (SEQ ID NO: 188), P-CUCme.CumMe_WSM_SF25936.G5450-1: 1: 1 (SEQ ID NO: 197), P-CUCme.CumMe_WSM_SF19839.G5090-1: 1: 1 (SEQ ID NO: 189), CumMe_WSM_SF206458.G5970 (SEQ ID NO:98) and P-CUCme.CumMe_WSM_SF Expression of GUS transgenes driven by 18716.G5860-1:1:1 (SEQ ID NO: 184). Each plant expression vector consists of a right-boundary region from *Agrobacterium tumefaciens*, a first transgene cassette, a second transgene selection cassette, and a left-boundary region from *Agrobacterium tumefaciens*. The first transgene cassette comprises an EXP sequence operably linked 5′ to a 3′ termination region from the *Codonopsis pilosula* FbLate-2 gene (T-Gb.FbL2-1:1:1, SEQ ID NO: 205) encoding a β-glucuronidase (GUS, SEQ ID NO: 206) derived from the potato light-induced tissue-specific ST-LS1 gene (gene bank accession number: X04753). The second transgene selection cassette is used to select plant cells conceived with resistance to the herbicide glyphosate (driven by the *Arabidopsis thaliana* actin 7 promoter).

[0209] The EXP sequences described above were cloned into plant expression constructs as shown in Tables 20-23 below, and then used to transform soybean plants using Agrobacterium-mediated transformation. GUS expression was qualitatively and quantitatively detected using tissue sections from selected tissues.

[0210] Histochemical GUS analysis was used for qualitative expression analysis in transformed plants. Whole tissue sections were cultured with GUS staining solution X-Gluc (5-bromo-4-chloro-3-indolyl-β-glucuronide) (1 mg / mL) for an appropriate duration, rinsed, and visually examined for blue staining. GUS activity was qualitatively determined by direct visual examination or microscopic examination of selected plant organs and tissues. Expression in Vn5 roots, R1 roots, Vn5 sarcopter leaves, Vn5 progenitor leaves, R1 progenitor leaves, R1 petioles, yellow pod embryos, yellow pod cotyledons, R3 immature seeds, R3 pods, R5 cotyledons, and R1 flowers was examined in R0 generation plants.

[0211] For quantitative analysis, total protein was extracted from selected transformed maize plant tissue. One microgram of total protein was used with a fluorescent substrate, 4-methyleumbelliferyl-β-D-glucuronide (MUG), in a total reaction volume of 50 microliters. The reaction product, 4-methyleumbelliferyl (4-MU), exhibits maximum fluorescence at high pH, ​​where the hydroxyl group is ionized. The detection was stopped by adding an alkaline solution of sodium carbonate, and the pH was adjusted to quantify the fluorescent product. Using a Fluoromax-3 (Horiba; Kyoto, Japan) with a Micromax reader, the slit width was set to excitation at 2 nm and emission at 3 nm, and fluorescence was measured at excitation at 365 nm and emission at 445 nm.

[0212] Tables 20 and 21 below show the average quantitative expression levels measured in R0 generation plant tissues. In both tables, tissues that were not detected are shown as blank cells.

[0213]

[0214]

[0215]

[0216]

[0217] As can be seen from Tables 20 and 21, depending on the EXP sequence used to drive expression, the EXP sequences are: EXP-CUCme.Ubq1:1:1 (SEQ ID NO: 1), EXP-CUCme.Ubq1:1:3 (SEQ ID NO: 7), P-CUCme.1-1:1:1rc (SEQ ID NO: 155), P-CUCme.2-1:1:1 (SEQ ID NO: 14), EXP-CUCme.4:1:1 (SEQ ID NO: 156), EXP-CUCme.5:1:1 (SEQ ID NO: 159), P-CUCme.6-1:1:1 (SEQ ID NO: 18), P-CUCme.8-1:1:2 (SEQ ID NO: 19), P-CUCme.9-1:1:2 (SEQ ID NO: 20), and P-CUCme.10-1:1:1 (SEQ ID NO: 155). NO:21), EXP-CUCme.eEF1a:1:1 (SEQ ID NO:162), P-CUCme.15-1:1:2 (SEQ ID NO:23), P-CUCme.17-1:1:2 (SEQ ID NO:26), P-CUCme.18-1:1:2 (SEQ ID NO:27), P-CUCme.19-1:1:3 (SEQ ID NO:167), P-CUCme.20-1:3 (SEQ ID NO:211), P-CUCme.21-1:1:1 (SEQ ID NO:30), EXP-CUCme.SAMS2:1:1 (SEQ ID NO: 168), P-CUCme.26-1: 1: 2 (SEQ ID NO: 33), EXP-CUCme. 29: 1: 2 (SEQ ID NO: 212), P-CUCme.CumMe_WSM_SF25355.G5000-1: 1: 1 (SEQ ID NO: 196), P-CUCme.CumMe_WSM_SF17111.G5790-1: 1: 1 (SEQ ID NO: 177), P-CUCme.CumMe_WSM_SF22531.G5120-1: 1: 1 (SEQ ID NO: 192), P-CUCme.CumMe_WSM_SF18488.G5340-1: 1: 1 (SEQ ID NO: 181), P-CUCme.CumMe_WSM_SF23760.G5200-1: 1: 1 (SEQ ID NO: 193), EXP-CUCme.WSM_SF19064.G5690: 1: 1 (SEQ ID NO: 185), P-CUCme.WSM_SF17252.G7330-1: 1: 1 (SEQ ID NO: 179), P-CUCme.CumMe_WSM_SF18634.G5190-1: 1: 1 (SEQ ID NO: 183), P-CUCme.CumMe_WSM_SF19647.G5760-1: 1: 1 (SEQ ID NO: 188), P-CUCme.CumMe_WSM_SF25936.G5450-1: 1: 1 (SEQ ID NO: 197), P-CUCme.CumMe_WSM_SF19839.G5090-1: 1: 1 (SEQ ID NO: 189), CumMe_WSM_SF206458.G5970 (SEQ ID NO:98) and P-CUCme.CumMe_WSM_SF18716.G5860-1:1:1 (SEQ ID NO: 184) quantitatively demonstrates the ability to drive transgene expression in some or all of the tested tissues.

[0218] Histological analysis of the selected tissue sections further provided evidence of numerous EXP sequence expressions. Although quantitative analysis showed fairly low expression levels, EXP-CUCme.Ubq1:1:1 (SEQ ID NO: 1) and EXP-CUCme.Ubq1:1:3 (SEQ ID NO: 7) showed constitutive expression patterns observed in all tissues. This type of driven expression pattern requiring constitutively low levels of transgenic expression is likely the most accidental. Expression driven by P-CUCme.1-1:1:1rc (SEQ ID NO: 155) was observed in the vascular bundles and xylem of the saphenous and progenitor leaves, as well as in the root bark, phloem, xylem, endodermis, stele, and apex. Expression driven by EXP-CUCme.4:1:1 (SEQ ID NO: 156) was observed in all tissues, with the highest expression observed during the reproductive period of the plant. Expression driven by P-CUCme.10-1:1:1 (SEQ ID NO: 21) was observed only in the V5 follicles and R1 anthers. Expression driven by EXP-CUCme.eEF1a:1:1 (SEQ ID NO: 162) showed the constitutive expression pattern observed in the yellow pod embryos and cotyledons. The yellow pod embryo activity in the R1 generation was 5-fold higher than that in the R0 generation (see Table 23 below). Expression driven by P-CUCme.15-1:1:2 (SEQ ID NO: 23), P-CUCme.17-1:1:2 (SEQ ID NO: 26), and P-CUCme.18-1:1:2 (SEQ ID NO: 27) showed histological constitutive expression levels. Expression driven by P-CUCme.19-1:1:3 (SEQ ID NO: 167) showed a histological constitutive expression pattern, except in the V5 roots and R1 petioles. R3 pods showed the highest expression.

[0219] Except for expression in the V5 root, expression driven by P-CUCme.20-1:3 (SEQ ID NO: 211) showed a histological constitutive expression pattern. The highest expression was observed in the cotyledons of the R8 stage. Expression driven by EXP-CUCme.SAMS2:1:1 (SEQ ID NO: 168) showed a constitutive expression pattern with histologically observed expression in all tissues. Increased GUS expression was observed in the R1 generation (see Tables 22 and 23 below). The R1 stage flowers and petioles showed the highest expression levels in soybean. Expression driven by P-CUCme.CumMe_WSM_SF22531.G5120-1:1:1 (SEQ ID NO: 192) showed a histological constitutive expression pattern with the highest expression in the cotyledons and embryos of the R8 stage. Expression driven by P-CUCme.CumMe_WSM_SF18488.G5340-1:1:1 (SEQ ID NO: 181) showed constitutive expression levels, while quantitative high expression was observed in yellow bean pod embryos.

[0220] R0 generation plants transformed with plasmid constructs containing EXP-CUCme.eEF1a:1:1 (SEQ ID NO: 162) and EXP-CUCme.SAMS2:1:1 (SEQ ID NO: 168) produced seeds, and GUS expression in R1 generation plants was analyzed. Expression in Vn5 roots, Vn5 saphenous leaves, Vn5 progenitor leaves, R1 progenitor leaves, R1 petioles, yellow pod embryos, yellow pod cotyledons, R3 immature seeds, R3 pods, R5 cotyledons, and R1 flowers was analyzed. Tables 22 and 23 show the mean GUS expression measured in each tissue of the R1 generation transformed plants.

[0221]

[0222] As can be seen from Tables 22 and 23 above, the expression driven by EXP-CUCme.eEF1a:1:1 (SEQ ID NO: 162) and EXP-CUCme.SAMS2:1:1 (SEQ ID NO: 168) in the R1 generation shows that increased constitutive expression levels relative to the R0 generation are observed in many tissues of the R1 generation.

[0223] * * * * * * * *

[0224] The principles of the invention have been elucidated and described. Changes in the arrangement and details of the invention may be made without departing from these principles, as will be apparent to those skilled in the art. All variations within the spirit and scope of the claims are intended to be included within the scope of this invention. All publications and disclosed patent documents cited herein are incorporated by reference, as if specifically and individually indicated that each individual publication or patent application is incorporated by reference. sequence list <110> S. Vlasinski BC Foat M. Offatole RW Schulz X. Wei W. Wu S-P. Yang <120> Plant regulatory elements and their applications <130> MONS:304WO <140> Unknown <141> 2012-05-11 <150> 61 / 485,876 <151> 2011-05-13 <160> 212 <210> 1 <211> 2611 <212> DNA <213> melon <400> 1 atctgaaagg aacacctagc aaggggctac tctacaagca tactaagtct acaaagctag 60 agttgtatgg ttatgcagaa gacctggaca aaagaagatc actcgctgct tttactttta 120 tcctaagagg aaatgtgatt ttatggaagt ttaacctata gcctgtagtg gcactattca 180 caacaaaagt aaagtttata gccatgactg aagttgttaa agaagtcgtc tggctaaaag 240 300 gtgcaataca cttgtctaaa aatctgcaat atcacgaaag aactaagcat attgatgtga 360 agctatatgt cattagagaa gtcatagcaa agaaaagt aacagtatca aaggttcaga 420 caaaagaaaa tgcagcagat atgttgacta aaatagttac taatgctaaa ctcgagcact 480 gcctacagtt gctcaaggta atagactact taaagaata gaatcaagaag aaatagtcat 540 tggtagcaat aaaattcaag gtggaggatt gttaaaaaga agagtgaatt ttattactta 600 aagaaaatct cggtgaaact cgaaagatatct cgattcgaaa ctctattgct taagaacctg 660 gtgaagctcg agagatcttg atacaatccc agtgccctaa ctcttcaaca agctaagcaa 720 gttgtactgt ggggctcaat ctcggttcaa tctcgacgca cctgatgctt tgttccctgt 780 ctactcgatg aagaagcaat tacttctcag gacaactcgg tacccctaaa tacagattttt 840 gagcttcgtg atcctacaac tgaaatcaaa tagaaaaact ataagttag ttagagtttg 900 960 gaaatataga atgaaaaaaaaaaaaaaaaaaaagggagagaa gaaaactcat 1020 ttcaattc tctatacttg tttgatcctt gaataagttg ataaaagct ctatggcggc 1080 ttcaaagtgg atgtaggcac tattagtcga accacaataa atttgttg ttctttgct 1140 attccttgta atctccataa atttttctt actaagctct agaaatctgc ttgtcaagag 1200 attaggtatc atttatgcct ttatattc ctttcggttg catatcttga gctagttaag 1260 atcgagaggt tactgttgtt gaaccgaga ttagtactt tggattaaca cgtgcctacc 1320 aaaatttgaa atttgttatt taccccattc attggattat aagcaatct tagtgtta 1380 tcattaaac tcctataaag tgtaatatt gatccatga actattttca tatgtaatct 1440 taaaaatg aatttagag tttattaaaatatatatt ttgtagcta ttttcaag 1500 tttgaagaat gtgttaattg atacacatac aaaaaatcta ggttttacat gaaaaactat 1560 ggaagtgaa gatcatct atattttat ghacaaat gcaactaat atattaagga 1620 tttattaat ttttattaggt ttcaatttg ttagacttgt caatacaa attttattga 1680 accaaataca tacaaacatc aaaattaaga acagaaaatc taaattcaaa tgaaatttat 1740 taatagaaaa attagaaaaa agaaaaagaa aataaaagga atcgtattgt tttttccttc 1800 ctttttccca tttgagaggt gaataaagct aattgagctg ctctaacttc ctaatcttta 1860 tgctttcccc ataaagcttt cccaactgcg cgtaatcgta taaatggaaa attgaccttt 1920 ccaactagat tcttccagaa ctaaacaata cgtaacacgc aagtaatcaa agacacgttt 1980 cattttccta tagaatatta tagttattcg tgattaacgg aagtcggcaa ttttaggtat 2040 aaatacgtga attctcgagc gctaattttc catacagact cgaaatactc taaactttct 2100 catcgcgctt tattcctatt tcgtaattcg ctcttcttca acctctcaag gttttcatct 2160 tttctctatc ttctgttttc agattgcatc ttttccccct cctgttcgat taattgatgt 2220 ttgaattttc gagaaacgat ttgaagtctt tgttgtattt ttcatttctg ttcgttaggt 2280 aggtcgattt ttaatcgtga tgtccgacgt tgttcggatg attcacattt ggtttttgtc 2340 atcttctttc tatgttgtga ttatcatgat ttttatcttt ttttctttc aagatttgta 2400 atttatcgat tccccatggt tcttggtttt ttatacatgt attgaatctg gttactagaa 2460 ttatgttctt cgacggacgt ctttcagatt taaattgcat tgtaggaaat atgatttgct 2520 atctgagtaa cgtttttcca gagtattctt gattgcgcga tctatcttca attgttaaat 2580 tgtttttgtt taattggggt catgacaggt g 2611 <210> 2 <211> 2068 <212> DNA <213> Muskmelon <400> 2 atctgaaagg aacacctagc aaggggctac tctacaagca tactaagtct acaaagctag 60 agttgtatgg ttatgcagaa gacctggaca aaagaagatc actcgctgct tttactttta 120 tcctaagagg aaatgtgatt ttatggaagt ttaacctata gcctgtagtg gcactattca 180 caacaaaagt aaagtttata gccatgactg aagttgttaa agaagtcgtc tggctaaaag 240 gactacttga agaacttggc ttcttttaac agtcagtaaa catcatgtgt gatagttaaa 300 gtgcaataca cttgtctaaa aatctgcaat atcacgaaag aactaagcat attgatgtga 360 agctatatgt cattagagaa gtcatagcaa agagaaaagt aacagtatca aaggttcaga 420 caaaagaaaa tgcagcagat atgttgacta aaatagttac taatgctaaa ctcgagcact 480 gcctacagtt gctcaagta atagactact tAAagaata gatcagaag aaatagtcat 540 tggtagcaat aaattcaag gtggaggatt gttaaaaaga agagtgaatt ttattactta 600 aagaaaatct cggtgaact cgaagatct cgattcgaaa ctctattgct tagaacctg 660 gtgaagctcg aggatcttg atacaatccc agtgccctaa ctctcaca agctaagcaa 720 gttgtactgt ggggctcaat ctcggttcaa tctcgacgca cctgatgctt tgttccctgt 780 ctactcgatg aagaagcaat tactctcag vakaactcgg tacccctaa tacagatttt 840 gagcttcgtg atcctacaac tgaaatcaa tagaaaact ataagttag ttagagtttg 900 ttatattac tgccattaaa taactgta atgtaataa taaaccattt aactcaatat 960 gaaatataga atgaaaaaaaaaaaaaaaaaaaagggagagaa gaaaactcat 1020 ttcaattc tctatacttg tttgatcctt gaataagttg ataaaagct ctatggcggc 1080 ttcaaagtgg atgtaggcac tattagtcga accacaataa atttgttg ttctttgct 1140 attccttgta atctccataa atattttctt actaagctct agaaatctgc ttgtcaagag 1200 attaggtatc atttatgcct tttatatttc ctttcggttg catatcttga gctagttaag 1260 atcgagaggt tactgttgtt gaaaccgaga ttagtatctt tggattaaca cgtgcctacc 1320 aaaatttgaa attttgtatt taccccattc attggataat aagcaattct tatagtgtta 1380 tcaattaaac tcctataaag tgtaataatt gaatccatga actattttca tatgtaatct 1440 taataaaatg aatttagaag tttaattaaa tttgtatgcta tttttcaaag 1500 tttgaagaat gtgttaattg atacacatac aaaaaatcta ggttttacat gaaaaactat 1560 ggaagtgaaa gatagcatct aatattttat gacacaaaat gcaactaat atataaaagga 1620 tttaattaat tttataggt ttcaaatttg ttagacttgt caaatacaaa attttattga 1680 accaaataca tacaaacatc aaaattaaga acagaaaatc taaattcaaa tgaaatttat 1740 taatagaaaa attagaaaaa agaaaaagaa aataaaagga atcgtattgt tttttccttc 1800 ctttttccca tttgagaggt gaataaagct aattgagctg ctctaacttc ctaatcttta 1860 tgctttcccc ataaagcttt cccaactgcg cgtaatcgta taaatggaaa attgaccttt 1920 ccaactagat tcttccagaa ctaaacaata cgtaacacgc aagtaatcaa agacacgttt 1980 cattttccta tagaatatta tagttattcg tgattaacgg aagtcggcaa ttttaggtat 2040 aaatacgtga attctcgagc gctaattt 2068 <210> 3 <211> 82 <212> DNA <213> Muskmelon <400> 3 tccatacaga ctcgaaatac tctaaacttt ctcatcgcgc tttattccta tttcgtaatt 60 cgctcttctt caacctctca ag 82 <210> 4 <211> 461 <212> DNA <213> Muskmelon <400> 4 gttttcatct tttctctatc ttctgttttc agattgcatc ttttccccct cctgttcgat 60 taattgatgt ttgaattttc gagaaacgat ttgaagtctt tgttgtattt ttcatttctg 120 ttcgttaggt aggtcgattt ttaatcgtga tgtccgacgt tgttcggatg attcacattt 180 ggtttttgtc atcttctttc tatgttgtga ttatcatgat ttttatcttt ttttcttctc 240 aagatttgta atttatcgat tccccatggt tcttggtttt ttatacatgt attgaatctg 300 gttactagaa ttatgttctt cgacggacgt ctttcagatt taaattgcat tgtaggaaat 360 atgatttgct atctgagtaa cgtttttcca gagtattctt gattgcgcga tctatcttca 420 attgttaaat tgtttttgtt taattggggt catgacaggt g 461 <210> 5 <211> 2002 <212> DNA <213> Muskmelon <400>  5 tcggtgaaac tcgaaagatc tcgattcgaa actctattgc ttaagaacct ggtgaagctc 60 gagagatctt gatacaatcc cagtgcccta actcttcaac aagctaagca agttgtactg 120 tggggctcaa tctcggttca atctcgacgc acctgatgct ttgttccctg tctactcgat 180 gaagaagcaa ttacttctca ggacaactcg gtacccctaa atacagattt tgagcttcgt 240 gatcctacaa ctgaaatcaa atagaaaaac taataagtta gttagagttt gttatattta 300 ctgccattaa ataactctgt aatgtaaata ataaaccatt taactcaata tgaaatatag 360 aatgagaaaa agaaaaagaa aaagttaaag agagagagga agaaaactca ttttcaaatt 420 ctctatactt gtttgatcct tgaatagtt gataaaagc tctatggcgg ctcaagtg 480 gatgtaggca ctattagtcg aaccacaata aatttgttat gttctttgc tattccttgt 540 aatctccata atattttct tactaagctc tagaatctg cttgtcaaga gattaggtat 600 catttatgcc tttattt cctttcggtt gcatatcttg agctagttaa gatcgagagg 660 ttactgttgt tgaaccgag attagtatct ttggattaac acgtgcctac caaatttga 720 aattttgtat ttaccccatt cattggataa taagcaatc ttatagtgtt atcaattaaa 780 ctcctataaa gtgtataaat tgaatccatg aactattttc atatgtaatc ttaataaaat 840 gatttagaa gtttaattta aatatatat ttgttagct atttttcaa gtttgaagaa 900 tgtgttaatt gatacacata caaaaatct aggttttaca tgaaaaacta tggagtgaa 960 agatagcatc taatatttta tgacacaaaa tgcaacta tatataagg atttatta 1020 ttttatagg ttcaattt gttagacttg tcaaatacaa aattttattg aaccaaatac 1080 attackaacat aaattaag aaaaaat ctaaattca atgaattta ttatagaa 1140 aattagaaaa aagaaaaaga aaataaaagg aatcgtattg ttttttcctt cctttttccc 1200 atttgagagg tgaataaagc taattgagct gctctaactt cctaatcttt atgctttccc 1260 cataaagctt tcccaactgc gcgtaatcgt ataaatggaa aattgacctt tccaactaga 1320 ttcttccaga actaaacaat acgtaacacg caagtaatca aagacacgtt tcattttcct 1380 atagaatatt atagttattc gtgattaacg gaagtcggca attttaggta taaatacgtg 1440 aattctcgag cgctaatttt ccatacagac tcgaaatact ctaaactttc tcatcgcgct 1500 ttattcctat ttcgtaattc gctcttcttc aacctctcaa ggttttcatc ttttctctat 1560 cttctgtttt cagattgcat cttttccccc tcctgttcga ttaattgatg tttgaatttt 1620 cgagaaacga tttgaagtct ttgttgtatt tttcatttct gttcgttagg taggtcgatt 1680 tttaatcgtg atgtccgacg ttgttcggat gattcacatt tggtttttgt catcttcttt 1740 ctatgttgtg attatcatga tttttatctt tttttcttct caagatttgt aatttatcga 1800 ttccccatgg ttcttggttt tttatacatg tattgaatct ggttactaga attatgttct 1860 tcgacggacg tctttcagat ttaaattgca ttgtaggaaa tatgatttgc tatctgagta 1920 acgtttttcc agagtattct tgattgcgcg atctatcttc aattgttaaa ttgtttttgt 1980 ttaattgggg tcatgacagg tg 2002 <210> 6 <211> 1459 <212> DNA <213> Muskmelon <400> 6 tcggtgaaac tcgaaagatc tcgattcgaa actctattgc ttaagaacct ggtgaagctc 60 gagagatctt gatacaatcc cagtgcccta actcttcaac aagctaagca agttgtactg 120 tggggctcaa tctcggttca atctcgacgc acctgatgct ttgttccctg tctactcgat 180 gaagaagcaa ttacttctca ggacaactcg gtacccctaa atacagattt tgagcttcgt 240 gatcctacaa ctgaaatcaa atagaaaaac taataagtta gttagagttt gttatattta 300 ctgccattaa ataactctgt aatgtaaata ataaaccatt taactcaata tgaaatatag 360 aatgagaaaa agaaaaagaa aaagttaaag agagagagga agaaaactca ttttcaaatt 420 ctctatactt gtttgatcct tgaataagtt gaataaaagc tctatggcgg cttcaaagtg 480<00gatgtaggca ctattagtcg aaccacaata aatttgttat gttctttgc tattccttgt 540 aatctccata atattttct tactaagctc tagaatctg cttgtcaaga gattaggtat 600 catttatgcc tttattt cctttcggtt gcatatcttg agctagttaa gatcgagagg 660 ttactgttgt tgaaccgag attagtatct ttggattaac acgtgcctac caaatttga 720 aattttgtat ttaccccatt cattggataa taagcaatc ttatagtgtt atcaattaaa 780 ctcctataaa gtgtataaat tgaatccatg aactattttc atatgtaatc ttaataaaat 840 gatttagaa gtttaattta aatatatat ttgttagct atttttcaa gtttgaagaa 900 tgtgttaatt gatacacata caaaaatct aggttttaca tgaaaaacta tggagtgaa 960 agatagcatc taatatttta tgacacaaaa tgcaacta tatataagg atttatta 1020 ttttatagg ttcaattt gttagacttg tcaaatacaa aattttattg aaccaaatac 1080 attackaacat aaattaag aaaaaat ctaaattca atgaattta ttatagaa 1140 aattagaaaaaaaaaga aaaaaagg aatcgtattg ttttcctt cctttttccc 1200 atttgagagg tgaataaagc taattgagct gctctaactt cctaatcttt atgctttccc 1260 cataaagctt tcccaactgc gcgtaatcgt ataaatggaa aattgacctt tccaactaga 1320 ttcttccaga actaaacaat acgtaacacg caagtaatca aagacacgtt tcattttcct 1380 atagaatatt atagttattc gtgattaacg gaagtcggca attttaggta taaatacgtg 1440 aattctcgag cgctaattt 1459 <210> 7 <211> 1507 <212> DNA <213> Muskmelon <400> 7 agtcgaacca caataaattt gttatgttct tttgctattc cttgtaatct ccataaatat 60 tttcttacta agctctagaa atctgcttgt caagagatta ggtatcattt atgcctttta 120 tatttccttt cggttgcata tcttgagcta gttaagatcg agaggttact gttgttgaaa 180 ccgagattag tatctttgga ttaacacgtg cctaccaaaa tttgaaattt tgtatttacc 240 ccattcattg gataataagc aattcttata gtgttatcaa ttaaactcct ataaagtgta 300 ataattgaat ccatgaacta ttttcatatg taatcttaat aaaatgaatt tagaagttta 360 attaaaaataa tatattttgt atgctatttt tcaaagtttg aagaatgtgt taattgatac 420 acatacaaaa aatctaggtt ttacatgaaa aactatggaa gtgaaagata gcatctaata 480 ttttatgaca caaaatgcaa actaatatat aaaggattta attaattttt ataggtttca 540 aatttgttag acttgtcaaa tacaaaattt tattgaacca aatacataca aacatcaaaa 600 ttaagaacag aaaatctaaa ttcaaatgaa atttattaat agaaaaatta gaaaaaagaa 660 aaagaaaata aaaggaatcg tattgtttt tccttccttt ttcccatttg agaggtgaat 720 aaagctaatt gagctgctct aacttcctaa tctttatgct ttccccataa agctttccca 780 actgcgcgta atcgtataaa tggaaaattg acctttccaa ctagattctt ccagaactaa 840 acaatacgta acacgcaagt aatcaaagac acgtttcatt ttcctataga atattatagt 900 tattcgtgat taacggaagt cggcaatttt aggtataaat acgtgaattc tcgagcgcta 960 attttccata cagactcgaa atactctaaaa ctttctcatc gcgctttatt cctatttcgt 1020 aattcgctct tcttcaacct ctcaaggttt tcatcttttc tctatcttct gttttcagat 1080 tgcatctttt ccccctcctg ttcgattaat tgatgtttga attttcgaga aacgatttga 1140 agtctttgtt gtatttttca tttctgttcg ttaggtaggt cgatttttaa tcgtgatgtc 1200 cgacgttgtt cggatgattc acatttggtt tttgtcatct tctttctatg ttgtgattat 1260[[ID=⑤]] [[ID=⑥]]catgattttt atcttttttt cttctcaaga tttgtaattt atcgattccc catggttctt 1320[[ID=⑦]] [[ID=⑧]]ggttttttat acatgtattg aatctggtta ctagaattat gttcttcgac ggacgtcttt 1380[[ID=⑨]] [[ID=⑩]]cagatttaaa ttgcattgta ggaaatatga tttgctatct gagtaacgtt tttccagagt 1440[[ID=⑪]] [[ID=⑫]]attcttgatt gcgcgatcta tcttcaattg ttaaattgtt tttgtttaat tggggtcatg 1500[[ID=⑬]] [[ID=⑭]]acaggtg 1507[[ID=⑮]] [[ID=⑯]]<210> 8[[ID=⑰]] [[ID=⑱]]<211> 964[[ID=⑲]] [[ID=⑳]]<212> DNA[[ID=㉑]] [[ID=㉒]]<213> Muskmelon[[ID=㉓]] [[ID=㉔]]<400> 8[[ID=㉕]] [[ID=㉖]]agtcgaacca caataaattt gttatgttct tttgctattc cttgtaatct ccataaatat 60[[ID=㉗]] [[ID=㉘]]tttcttacta agctctagaa atctgcttgt caagagatta ggtatcattt atgcctttta 120[[ID=㉙]] [[ID=㉚]]tatttccttt cggttgcata tcttgagcta gttaagatcg agaggttact gttgttgaaa 180[[ID=㉛]] [[ID=㉜]] It should be noted that there may be some inaccuracies in the translation as the original text seems to be a DNA sequence with some unclear or potentially incorrect notations. The translation is done as accurately as possible based on the given rules.ccgagattag tatctttgga ttaacacgtg cctaccaaaa tttgaaattt tgtatttacc 240 ccattcattg gataataagc aattcttata gtgttatcaa ttaaactcct ataaagtgta 300 ataattgaat ccatgaacta ttttcatatg taatcttaat aaaatgaatt tagaagttta 360 attaaaaataa tatattttgt atgctatttt tcaaagtttg aagaatgtgt taattgatac 420 acatacaaaa aatctaggtt ttacatgaaa aactatggaa gtgaaagata gcatctaata 480 ttttatgaca caaaatgcaa actaatatat aaaggattta attaattttt ataggtttca 540 aatttgttag acttgtcaaa tacaaaattt tattgaacca aatacataca aacatcaaaa 600 ttaagaacag aaaatctaaa ttcaaatgaa atttattaat agaaaaatta gaaaaaagaa 660 aaagaaaata aaaggaatcg tattgtttt tccttccttt ttcccatttg agaggtgaat 720 aaagctaatt gagctgctct aacttcctaa tctttatgct ttccccataa agctttccca 780 actgcgcgta atcgtataaa tggaaaattg acctttccaa ctagattctt ccagaactaa 840 acaatacgta acacgcaagt aatcaaagac acgtttcatt ttcctataga atattatagt 900 tattcgtgat taacggaagt cggcaatttt aggtataaat acgtgaattc tcgagcgcta 960 attt 964 <210> 9 <211> 1022 <212> DNA <213> Muskmelon <400> 9 tgacacaaaa tgcaaactaa tatataaagg atttaattaa tttttatagg tttcaaattt 60 gttagacttg tcaaatacaa aattttattg aaccaaatac atacaaacat caaaattaag 120 aacagaaaat ctaaattcaa atgaaattta ttaatagaaa aattagaaaa aagaaaaaga 180 aaataaaagg aatcgtattg ttttttcctt cctttttccc atttgagagg tgaataaagc 240 taattgagct gctctaactt cctaatcttt atgctttccc cataaagctt tcccaactgc 300 gcgtaatcgt ataaatggaa aattgacctt tccaactaga ttcttccaga actaaacaat 360 acgtaacacg caagtaatca aagacacgtt tcattttcct atagaatatt atagttattc 420 gtgattaacg gaagtcggca attttaggta taaatacgtg aattctcgag cgctaatttt 480 ccatacagac tcgaaatact ctaaactttc tcatcgcgct ttattcctat ttcgtaattc 540 gctcttcttc aacctctcaa ggttttcatc ttttctctat cttctgtttt cagattgcat 600 cttttccccc tcctgttcga ttaattgatg tttgaatttt cgagaaacga tttgaagtct 660 ttgttgtatt tttcatttct gttcgttagg taggtcgatt tttaatcgtg atgtccgacg 720 ttgttcggat gattcacatt tggtttttgt catcttcttt ctatgttgtg attatcatga 780 tttttatctt tttttcttct caagatttgt aatttatcga ttccccatgg ttcttggttt 840 tttatacatg tattgaatct ggttactaga attatgttct tcgacggacg tctttcagat 900 ttaaattgca ttgtaggaaa tatgatttgc tatctgagta acgtttttcc agagtattct 960 tgattgcgcg atctatcttc aattgttaaa ttgtttttgt ttaattgggg tcatgacagg 1020 tg 1022 <210> 10 <211> 479 <212> DNA <213> Muskmelon <400> 10 tgacacaaaa tgcaaactaa tatataaagg atttaattaa tttttatagg tttcaaattt 60 gttagacttg tcaaatacaa aattttattg aaccaaatac atacaaacat caaaattaag 120 aacagaaaat ctaaattcaa atgaaattta ttaatagaaa aattagaaaa aagaaaaaga 180 aaataaaagg aatcgtattg ttttttcctt cctttttccc atttgagagg tgaataaagc 240 taattgagct gctctaactt cctaatcttt atgctttccc cataaagctt tcccaactgc 300 gcgtaatcgt ataaatggaa aattgacctt tccaactaga ttcttccaga actaaacaat 360 acgtaacacg caagtaatca aagacacgtt tcattttcct atagaatatt atagttattc 420 gtgattaacg gaagtcggca attttaggta taaatacgtg aattctcgag cgctaattt 479 <210> 11 <211> 716 <212> DNA <213> Muskmelon <400> 11 tcgtataaat ggaaaattga cctttccaac tagattcttc cagaactaaa caatacgtaa 60 cacgcaagta atcaaagaca cgtttcattt tcctatagaa tattatagtt attcgtgatt 120 aacggaagtc ggcaatttta ggtataaata cgtgaattct cgagcgctaa ttttccatac 180 agactcgaaa tactctaaac tttctcatcg cgctttattc ctatttcgta attcgctctt 240 cttcaacctc tcaaggtttt catcttttct ctatcttctg ttttcagatt gcatcttttc 300 cccctcctgt tcgattaatt gatgtttgaa ttttcgagaa acgatttgaa gtctttgttg 360 tatttttcat ttctgttcgt taggtaggtc gatttttaat cgtgatgtcc gacgttgttc 420 ggatgattca catttggttt ttgtcatctt ctttctatgt tgtgattatc atgattttta 480 tctttttttc ttctcaagat ttgtaattta tcgattcccc atggttcttg gttttttata 540 catgtattga atctggttac tagaattatg ttcttcgacg gacgtctttc agatttaaat 600 tgcattgtag gaaatatgat ttgctatctg agtaacgttt ttccagagta ttcttgattg 660 cgcgatctat cttcaattgt taaattgttt ttgtttaatt ggggtcatga caggtg 716 <210> 12 <211> 173 <212> DNA <213> Muskmelon <400> 12 tcgtataaat ggaaaattga cctttccaac tagattcttc cagaactaaa caatacgtaa 60 cacgcaagta atcaaagaca cgtttcattt tcctatagaa tattatagtt attcgtgatt 120 aacggaagtc ggcaatttta ggtataaata cgtgaattct cgagcgctaa ttt 173 <210> 13 <211> 2000 <212> DNA <213> Muskmelon <400> 13 cttattcagc gctttcctgt aaaattaaag acttgatgag ggagaaaag aaaaccggtt 60 cgcagcttca agaagacggc ttccgaataa gatcagata ctcgatgatg gggaaacaat 120 aaaaagata tcaaagaa tcatgaaca tagcataga acgaaaccc agaggtgag 180 aacagtgccc aaacgcaact ttacccaag aacatgtata aaacgtctc cagacgttca 240 aaatagaaa gtgacaaaa tcaagctac aaacgatctc caataacag atggaaaaca 300 ctaattgcac tagagatttt gatgctttg ttgttgattt ataatcctcg acttccaaga 360 aaagtaca aggaaatg acgaatcag atccgcaatc gagatctga aggaagta 420 aggtaggct aaagaaccat aggaaacgg taaaaacgtc caaaacagtg tgagaatat 480 cgcagattca aaggtccgaa ccctaagac gtgttatgc agcttaaag gtgagaatca 540 aaaccctcta tccataacgt ggacggcgcg gttgaatcat tgtcttgttc cttgaaactg 600 aaggtatgcg agacatagaa ttcgatctca ctattatctt ctaatcaacg acgaagtaaa 660 gagtgaat ccagaacaaa gaatggaga ttggaatga caagaaaac ggcagagga agtggaaaag tgaaagcgga ctcacctaga tcaatgccct tggctggtcg agcttcagga 780 acctgtcgtc ggagagaaag agaaagagaa aagagcaaga gagagagagagagacacaa 900. tggaggcttt tgtttcgata ctccctgatc tggaattcta father actataact tctctgggtt ggcccatcat cacgtatatt gggcttttag cccaattatt tgttcactgc tcatgggccg gtgattttgg gctttcttct gggccttggt 1020 1080. acataacaac ccagtatatg acgtattttc ggtgatagct attttcaaga acaccaactt ttttgttcaa caatgtggag atcaaataac agtatgtata tatacacaaa catatgctca 1200. 1200. 1200. 1200. 1200. 1200. 1200. 1200. 1200. 1200. 1200. 1200 ctctttgttt gatttcatga acggtgtgtt ctataaaa caatgaaata acataattat 1320. cttaaaacat gatgatttca atattcatgg tttacatttg gtgggatgat tcgtttaatt attattgata atgtatagtt attgtgtgtc ccgttttctt tttctttggt 1380 ggaagaaaag aaaaaagtag gaaggcatgt aatattgcga tccttcacgg gacagatcca 1440 ttttccaatg tgatcgagta ctagttaggt ggagagtgga agaatcttcg tgcatgcata 1500 aatcaagtca caacttgcca atttggaaag aatcatgtta tattctacct ttactttcaa 1560 gtagggttaa gtgaattaga ccacaacgaa gcaatcaagt ccaaccaaac tcacttaggt 1620 caagcagttt agtgatatag acaggtcagt ggtcgttttt ttaatactaa gaaatgtcaa 1680 ttctatctag ttgactatta ttcatataga aggagaaaaa tgataactat gattgtccca 1740 caaacaacaa ctaccgatcg atctaaacca acaagtcgat gattggtgcc actttaaatt 1800 taaatctgac gccactcaat tgatgatcac ccctattcaa gcacacaaga acgcatgctc 1860 ttaaaacatt tggtaatatg attggaatta gtacaaaatg tttattcgat ctatacaaac 1920 aactcctttt taacacaaat gttttattgt actttcccgt gaaatggggt tagtaaaact 1980 atggagttaa taaaacataa 20​​​​​​​​​​​tataacaaaa tatgtgaaat tagccattat gtttgtcctt tcgttcttct tattcacttc 60 gttgcgattt ctttctatcg tctatcgtct ttctttttt ttctgttgaa atttattttc 120 atcgtttttc ttctttttcc atcgtgaaaa aaatagtcaa atctaaatga tcgtgtataa 180 agaataaacg atcgtgtaga caaatctaaa tggtcgaata ttaagaaaat tgataggaaa 240 atttattcat tagaaaaatt ttagtaagaa aaattagaaa tgaaagggtt gaaccagaaa 300 gaaataaaag taatagacaa atgaaaattt taaataaaaa gaaatttggg atgggtgcat 360 ttactattta gtcttgagtt ttaattcttt tattacttta cataagatgt attaaattaa 420 agaggtaaga tagaattttt ttttaaaaaa aactatcatt agtaaattta acaaaagtga 480 catagcacca ttttcgttaa aagaataatt gttttatgta gtaaaattgg tagaaatatt 540 ttttaagtat agcaaaatat ctttgtcttt ttatatcttt cactgacaga taataataat 600 ttattaatat atcatttata tagtcccatt ttcggtaaat tttaatattt gaacataaaaa 660 cactatttaa aataatgaaa aaaaacttta caaactttt tatttttat atatttgtaa 720 attatctctaa aaaattttac atttaaaata atattttcaa ggttaataca gaagaaaaaa 780 aacaaaaaaa gaggaaaagg caatttaaga agaatgacaa gaaaatcggg aggtggtgtg 840 gctaagagga agaagggacc ggttcttcaa gatccaacgc tccacattca atctcacttc 900 cttcttcaat tccgtctttct cgtttcctc cttatatgc ttctctcttt ccctcccttt 960 ctttctctcc ttcaatcaat caatcaatca atcaatcaat cctcccattc cattacatt 1020 gccaaaaggt tctattctca ttctctacat ccatttccct ttctttcctt cttcttcctc 1080 tgttcttct tcgtttcctt gattcatttc tctttgtacg ttccttcctt cttctgcat 1140 tttgattatt ttcttttgtt ttacgtccgg aattgcaatg tggtttatct ttatttctgt 1200 ttttggacgt caagatgcct gttgttttta acattttgat ttgattcatc gttcatggcg 1260 taatcatgtc ttttggaatt gtttgaaatc caaggatcac attgatttca ctattgtttc 1320 atttgttct ttttgttaat tttgtataat gaatcgtata ggggatcatt tttccattgg 1380 ttctcttgaa aatctttaag agttgcatta tgtatactaa gtctctctta tggcgtctgt 1440 ttgagtgaga attgataaaa gatccatggg aggaagaagt tttctttcat gaggcttggt 1500 tttattcagc tgtttcttct cgttgcaatt tgttgaagaa gggacatggg tatcttttac 1560 atcaaagtat aactaactat ataattcaat ttggttgata aagtagatac atgtaggagt 1620 caaccgattt gagtgtataa taatgttgtt atgtcccttg caacttaatg tagtgcatat 1680 ttgggagtga ttataaaatt gtataaatca ttttatgttt agaatcatct tgaaacacgt 1740 tttttagtat ttaaaaacta atttaatatt tagttttgca cttttaaatg aaatttttgt 1800 ttcactaata ttagttttga ttcattaaat gcatgctcca tcgtaatatt aaaagtaact 1860 agtgatttta accattttat aatcacatgt ctgtgatata gtgaagtgta cggctgcctt 1920 gttgagaatt gttacccttt agaagaaaca caggatgtat ttgatgttta acttgcattt 1980 tcttctggca ggcttagaaa 2000 <210> 15 <211> 1990 <212> DNA <213> Muskmelon <400> 15 tatttgtaca atgaaaatat ttatttcttt tctcgattct ttaacaaaag ttcaaaatct 60 tttatcataa atacaatat ttagtaattt aagtttagac taggtgtatc agatgtgcac 120 caagtgtata tcacgtgcat caagtatata tcaatgtgt accaacgta tatcacgtgt 180 atcaagtgtg tatcaagtgt ctatttgaag tcaagtgcat windowata tcacatgtgt 240 accaaata tcaaataa tactgattga gcatcaagta tgtctattag tagtgtatca 300 agtgtattaa ttaagtttgt agcaaggtg tatcatgat tataacgtgt attatgtggg 360 ttggttttttttttttg tcattttgc aaagtaatt aagtttgtgt tatgaaccta 420 attttttaaa tttcttttg tcacgtataa gagacttga ataggttta aaggtctta 480 agggtatttt agtttgactt ttttaaaag tatttatg attattaaaa attagaattt 540 tttagaaaga ataggatttt tataaattat tctttaga aaaattgcat cagatgacaa 600 aaaaaattta gaaatagca gcccataata actctttaaa ttgctatca gacgactatc 660 cgagggttat catcttttaa atttgctact tttacaattt agaaaatgta gtgacatgga 720 ccctattatc ataagatttt ttttgctat ttttgcaac acatgttctt ttaaaatgac 780 ataatttatt aaaaaaaaaaaagtta tttgatgat cttttgacc tattttttaaa 840 agctaaagta ctaaaagat acatattgaa aacttgaggt caatggggct attattata 900 atatgtggac taaaaatgta cattctaa acttagagac taatgcaca tatttaaaa 960 agcatgtgaa ctaaaaaagt cgttttttccct atatttttt tacaacaatg actaaattga 1020 acccaatt tgaagggtgg aaaaccatac taattattca ctaatgaact aaactcattt 1080 gatgatttca agacatatga gttcattga gtagttggtt tgagggat gaatgagtg 1140 gtggaagaaa gtttatgtaa cgaccaacg aaataggaag gtcatcccaa ggaagtcgca 1200 catccaatga gtattacca caaacaacc tctccttttt tctcaatttc ccttttaata 1260 aaatttga ttccccattc cttccttctct cccttggcag ccttcctt ttttcaagg 1320 tttttttttttttttttttttttttttttctctttttttcttca 1380 1440 tcttttctca aagttttgtt ttcattgat gtagatgttt tgtatcaat caatggaat 1500 ttgagttttt cttatctcat tgtatcatca ttgagtgtgt gtttatgtta gggatccatt 1560 attaggatgg atgagaatca taatttcatt gctaatctat gaaccatgaa taaagaaatc 1620 taaatccaac atagaagata gaacatttgc attgtgttat gagtaaccag ctctgtcact 1680 tcaattggtt cttctacaca tttgatggca atggctttgt ttgatattcg tgatggcatc 1740 taagcattgg ttcttcctat gtttttcgtt ggctcttggt ttgatttgca attagtgaag 1800 agcatgtttg gaatgaatga gttgaaatca cctttaacat ttttaaaatc actttaaata 1860 ttaaattaat tttgagtgat aaaagtaatt ttaacaatga taaaattact ttcaaatgtg 1920 ggccgaatca aattgtctag aatgtttagg gttctccaac taatagcaat ttatccaaac 1980 agggtaaaaa 1990 <210> 16 <211> 2005 <212> DNA <213> Muskmelon <400> 16 ttgttagagg agttagcttt tcagggagtc ggttacaact tacaagacag acaaccatac 60 tgtacaatca atcactactg cctcaaagtc gaaaccattt aataccagtt gagcctcatt 120 gtgactgcat aattttgacc cctaccacga ggtaatttca gttcaatca attgtatctc 180 tcttctggat atcagtcag aatctcatgt tctcagtta tagtacattg attackg 240 tgcatttta ttaatttatg agttaaaatc ctgctgatta attcaactaa aggaataaaa 300 tagttattgg cattttgtg agaatagaat gtgaatccca tcattcatcg ctagattgga 360 ccgtattat aagtgagagg gagaaacttc tgttgctatt cccttttat ttctttattc 420 attataaat tgttttagg ccttttatat atatatattt ctaccatttt tacatttaaa 480 attcttttaa ctttattatg tatggactca actaacaag ctttattga taaaattgtt 540 siaactatta tattagtttt atatttgtaa accataaac aaatccataa atttccacct 600 gcatcactca ctcatgttgt tgaatggtac gaaataaaca atacatgtga aagatgaaaa 660 taagaattgt tctcttatta aatctaaaat ctagattttc tttttagtac attack 720 tcaatgaaag gtctaaaaca ttattgaatg acgtcacgaa ctattacaa agattattcc 780 gatttatctc aaaaggggtc tatttcacta atttggtgt catcatg windows 840 tttcgtgata tgtgtatagata tttaaatata attgaattcg atgaaagcaa agcaagaatc 900 gatatccgta gttattttga tatagatcgg tgataaataa aagacaatat gcataaagtt 960 tgtgggtaca gagtcggcta gttgcaatga ggggtatggc cccaagactt ttccccaacc 1020 ccaacggtca attaatcacc ccaatggggc atcgaatctt ccccaccatt ttccttttct 1080 tcgccgactc ttctacccat ctcttttgcc gactctttct cacaggtttg attaaatccc 1140 attcatattc agatacacta tttcaaata actcgcaaat taatttgttt tttaaatatt 1200 ggtataataa taaaattaat tataaattgt gacctaaaaa gtactttgtg gaagaggga 1260 tatagtctgt ctaatatatt attgattaaa tatataatag aaccataaat gcaaaccact 1320 agaattgtta ataaaaatca acgctctttt aagtctttcc atagaaagaa aggcaaagac 1380 gcgcaacgtc tagaattttc tttataacgg aagctaactc tgttataacg gccgtccaca 1440 taatatggta gatttaaaat gacgtcagca tgtcgcttct aaatttgggt ccaaaggggg 1500 gattcattta tagggaaggg aaacggcaaa tgcaagcata gtgagtaaat acgtggcggc 1560 aaagtaatgg ataattctgc ctctgccgtg acgacattgc ccttccattt cactataaat 1620 acacctcctc tatccctcga tttcttcgca attgaatttc tcttctcatc tctgtcgtag 1680 caaaccaaat cgatttcttc aaaggtattt cttcctttcc tttttttttt tttttttttt 1740 tttttaaatc atgttgttca aactttgaga gatgaaatga ttaggggctt tcaaagtggt 1800 tttcgtttga tatgtttctt agatcgatag ggtttagaat cgagcatcct tgtaggtatc 1860 ctgaggtttg gtggttggat ctgcttaatt tttatgtggt tgcatggaaa attgggattt 1920 tttttttcta attacgtgat tctggaaata ttgatctgtg gttcagatgg aattgaatct 1980 gatctttctg ttttgttctg tatag 2005 <210> 17 <211> 2004 <212> DNA <213> Muskmelon <400> 17 tagaaaaaaa ttgaagatct tcttaatgct aataataaaa gcatatataa aaaggcttca 60 tattacacaa gcatcttaaa accaaaccga acttgatttg aaattatccc actaattctg 120 tagatttcac caaagtcctt aacctttata ctaacatctg catttgactc tttcatttga 180 cctccaacat attctttctc atttccttc cattcaccac aaaaaccaac aaatacaaaa 240 aaccacaat acatagaaa tataaaaaaatcaaatt tcgagatgaa atcattaata 300 actcatccga taacttgag atttgaaacc ttacactata taagaaact catccgataa 360 ctttgaattc gcatcgaaat tacgtaagtg aagacgaaaa tgtaatgat tagatgcgaa 420 taacaaaaaa aacataattt ctaaacgtaa aacactatat tcaccttatt atacttgatt 480 attttggaaa agtatgaat tgagtgtgg gagaggagcc aaagaattgg aaacttgtta 540 ttaggagtcg tttgaaca tttcacaa gccaatattc ttcacatac tataatgata 600 tacatagaaa taatacaata atatttttga attgaggca ttttgtcgt atttatcta 660 aaaatgtcag ggtagattca tcatgtatac aaatctctcg ctatcaaac tatataaaaa 720 tcttgtgaat gatttcaatc gaatggacc gagaaaaac atcgtaacca cctctaaaat 780 cgataaattt gttcaattt caatcccta aaactaaagg tgctactttg tacaatttcc 840 cctgattagg gtgctaaagt taaaccctaa aaaggtgt gtacgttttcc ggaagttctct 900 agaatcccca gcgaagttct ccaaatcgtg gcttgcagac caaggacccc cattaaaatt 960 cgtttcttcc tctaaacctc ctcccttaat tttggcattt tggtattttg gctcctataa 1020 attcacccc tccttatccc taatcctttg tcttccaaat tttccttcaa agcctgcttt 1080 tcccatttcg tcgtgctttt tcttcatcta aaggtatatt tcagttctag ttttctttct 1140 ctgttgatct cttggatttg agggacgttt gaagttggct ttgtttaatt ctttgttatt 1200 caatctcttt ttttgttaga gttgttgttt aatcgtttcc cttgttgttt ttctcccttc 1260 tagttcgatt ttagaacgct ttttgtgggt tgattttaat ttctccgttt tcttacatct 1320 ttcacaaaga aacgattgaa atcgtgtttg ttttttttcc cacggcatac gttattagat 1380 cttgttagata atgatctcaa tctattgttt agtttttgca aataagaagt tggtttttta 1440 tctccaactt ttatatattc gattcgatga gatgttctac accgttagga tggaaccaag 1500 1560 tttttaatgg attgtataat tcgttcaatt ccttgtcgtt ccatttttat ttctgtttcg 1620 tttttcgtgt tgctgcgtat cgcttccctt gttgttttcc tcccctattg attttgcgtt 1680 tcttggagtt tctctgtttt ctctcttcat ttttctacaa aaatcaattc tatttttatt 1740 cgttttcaat tcccgagctc cttggaatgt tatccttttc tcctgtgtaa ataagaaccc 1800 gtattcaatc ccagttcata gtttggcttt cccaaataag agcaaaaaga ttgtactgag 1860 aagttgaaga tttcaaaatt ttgtacatga tttcttctaa tttatcaatt tgattggact 1920 ttttgtatat agatttggtt cttgagctat ttatgttatg acgttttcat attgaggcca 1980 tgcgttgaat tggtttctta acag 2004 <210> 18 <211> 1935 <212> DNA <213> Muskmelon <400> I8 tatacaaatg acaaaatatc gttgaagtcc aaaaaagatt tattgttggt aaatatcgtt 60 aaagttagta aatagatttt agagaaagga gatatagccc ttgtagtaga aacacacaca 120 caaattgaat tagatgtgtt taatgtttaa ttaaattaga tatgagtcaa cttatatcta 180 atataggaca ttattaaaca aataagaaat aagaaacatg aaacaagaaa aacaagaaat 240 agaaacaata tcaacat tcctatttct tgttctaaaa aaagaaaaaa catggtacaa 300 gaataggaa acggaaaga ggaaaagg aaaaatgct accaaacggg cctaagtttc 360 taacaaatg agctaggtgt agtttattgg tatagatagt gactttcaat tattttaat 420 ttttttacc atacctccac gtctttagaa tcttttat tattatgtga tcttattca 480 ttcatgtctc aatcttaaaa ttagaacatt acatgttcat cattttttcc tttgttact 540 gtgtttaatc tttcctaca agacaatag tttacctta atccacacat tattatacc 600 aaatttaaat atctacctt siaagaaac attattatta tcttata accacaatt 660 ataataccaa actctaacgc tccaaccaa cctaggaga atgacaagc tgtcataatt 720 tagttggttt ggcacgttgt tggagttct caaattatg gaaatattta tttccttctt 780 ctttatccat catcctcctt gggaggggtga atttgtgtta aaaagaata gaactaaag 840 tctaagtggc aggacttaca ttatgtgtgt atgtggaagt aaattgcag taacagttta 900 caaaaacaac tcatccatga ttcatacca acttaaatga atattatttt ttgcctaaag 960 atttaaatt aatatataag cggaagaatt aacctataac ttcaagttta acaacacaaa 1020 tattatatca tactgattaa ttattggaat gatgtttagg ctttaaacat aaagtattga 1080 gaggctaatt tgagtttaac tcactaaact atcattaccc tttcaaaata gatccaatca 1140 tccatttatt ataatactca atgaaataaa gcaaaagatg agtaaaataa ttcaccatga 1200 acattgataa ttaatttcc cactaagata aactactact cctcaaatct tcatatgtgt 1260 ttttccttt tgagttgcac tcaaatttc atagttgaaa tttacccatc aaaacaacca 1320 acaatctttc aaattcaaca aacatttgac cttacaccct ttgatgccaa atccttaccc 1380 tctccctctt ccataaaaat tcttatataa accaccatca ctctcacttc tcaattcact 1440 ctcttctcta ctcccaatca cctgacttgc ctcttactcc accgccaggt tccgccccaa 1500 cttccccggt aagttccagt tcttcagatc tggttaccac atttgatttc ttgcttgtat 1560 ttgacgtggg aattttcata tcggcgtttt ttcgaactgg gttttgcttt atgatcatat 1620 tcttgtagta aaatgccatg aatctgttat ttgattccgt ttttttgga gatcggtcta 1680 gctttatggc catattctgg catttaaatg ccatgaatcc gtgatttggt tgaatttcac 1740 ttccgatcca atgtttatgc tgatattgac atctttgcat tcaatgcaga ggagttttgt 1800 ttcgatttat tactgatctc atcacactga tcttgaattt tttatacttt tatgtgtgtg 1860 tgtgtatttt ctttaatatc tatgccaatt gaactatgtg gttaacttca gagtgttctt 1920 gtgggcagtg agaag 1935 <210> 19 <211> 1606 <212> DNA <213> Muskmelon <400> 19 atatattgta tcgattcttt agttgctcta tgtttttgtt tgcttcattt gtcgattaaa 60 ctgtaaaatt aatttctttg acaaggaaaa agatataatt taattctata atttattaca 120 atctaatcca tatggtttaa taaaacactg aaaattgttt atgaaaattt tatcgaacta 180 caagaactat taataaagtt tttttaaacc gtaaattgaa tgaattttct ccacggtgta 240 aatttgaaaa cattaattaa ttaattaatt aattttaatt tcaaggtttt ttctgaccca 300 tgaacctatt ttatgatata agttgttcag gggttgcaat agtaaccaaa taaagttgat 360 cagaaaaggt taacaactca tgaaaacttc caaatgcatt tgtgtttcaa ttattttctt aaccctcttt ttttggtaat tttagtttaa aaagtgagtc ggttgatcat tattgttctt taatttcttg ggagaaaaat attaatgttg attatggtga tgagttaagt ccaattcttc atcaatcat accaattag gaacaaaaaa aacatcaatt ttaaggtgca aatccattc 660. taatggctaa aatgtcaagc atcccaccaa accaacaatc tctaaaccca tttttactcc actaatctaa tgtttaataa taatcaaaaaa ggttttgctc attccttttt tagtaataa tcatttaaca ccaaagctca aaagtaccca cccaatggat caaaatcgag aatatagc atttaaggat ataaagacta gagataata taacctagct tagagcttaa aggatacac tagccat gtcaatttgg tagcaatct aaaaacaaat aattcgatga aaataaagtt gtattttgt gttttcaaac atgttttaag acgaaggttt ttgataaatt tgatctcaat aggtaacaa tggtaattac tcgattaa ttactcacta aataccaaat cgatata ttattactaa ttattatga acatgtttta cattttaaaa aatgaaat ttttttttta gaatttgtgt tattgaaaat aattttcaaa acaatattga atgaatctta agtgaaatca 1140 atgtattaaa agaacataaa acataatcta gatggtctat cgaacaagct agaaaatatc 1200 ttccataaat ccaatgatta agacaggcag gcaggcatga agataagagg attggattaa 1260 ttggtgattt taagttatga ataaagacac aagaactagc agctctcctc ttcttgtcac 1320 cttcctttgt catccagctc acacaactcc aacttggaat ttgacaggtc tctcttcact 1380 catacattcc cacatgaaat tattaattga atcttcaaca ttgtctttga ttcttcagct 1440 gcactgtcct tttccaccat ttttttcttc aagataaaga ctaataaact ccttatatat 1500 tcctctcttc ccattcacct gtgcatactc acaaagcaac tgccatttcc ttcttgttta 1560 tctctgtttt tttcttacac atttgttgaa ctttccctct gaaaaa 1606 <210> 20 <211> 1487 <212> DNA <213> Muskmelon <400> 20 taatgaattt gtatttgtta gtggattgag tttatatgat attaattttg acccaaacag 60 ttgagtacgt aattaatgtg gcttgcattg aaagtgatat gggcatatag tatgtgtaga 120 atgtagctga cacaacacat taacaaaacc caattttaac tttttctttt tctttttctt ttattttat atggatcaga tcacatgtca ttttccatta caactcactc tctaccaatc atcccatccc ataggccata ccccataca tccctttcta aatatctaa tcatctccct 300 aattattac attttttttc tctcaaatat aactattcaa ttcataaata ttattctttt tttagctctt attatttcaa ttatgatttt aaatattcct tttcaattta cgacctttta tttaccatat caacatttta attctactca attaagatc attaatga aatttcaggt father aaataggtgt father tggactacta atttcactaa tttcgtcatc tgaaatagg acaagttcca actatcacta ttgtgaaac ctcataactc ctaaaagtgt taaaattgga ccctcaagtt father ttgcaaattg aatcccaaaa father agtataattt atacgttttg agagtcaaat ttaattttg aataagcttg aatacttaac ttctaatttt gaaaatttaa aaatgcaact gcgagagtaa cttttgcaat tagccgtcga aacaattaat tatattggtt aatttatgtc tcattctctt ttgatgacca taaagataaa cccatttata atataaatat caagcaaagc taaaacaaaa tctttttttt ttcaaattag 900 atctaaatat gaataaaagc agaactttct agaagtacaa atttgattat ttttcttgag 960 ataaaatttt cgctatgaac ctttttataa taggaaaaag agaaaaagga tggttttata 1020 taaatgtatg ataaaaaggt aataatatcc attgtaatag taaaaaagaa aaaaagaaaa 1080 aaagaaaaag caattttctt tttcatgatt aggaaatata aaaacaaaaa ttggctccca 1140 attgacatct ttaatcttct ttttcttttt cttagaaaat aaaattagtg agagaaggaa 1200 aaaaacgaag ggttgagaga tagagagaga aaaaattgat ttttaattta gtttattttc 1260 cttttttgga gcacaaaata aatagataaa taaaatatta gtttgcaaaa aagcccctcg 1320 agtttatctt atttgctcaa aaaagcaagg ataaatacct cccgacatcc ctgtttatcc 1380 ctctcagttt cataattcca ttggttcgat aagaaaacaa ttctcccaat attcccgctg 1440 tagatctcgt cgattttccg tttgtttccc gggaagatca atcaaag 1487 <210> 21 <211> 1448 <212> DNA <213> Muskmelon <400> 21 gtgtcttgt tgtaggaaa tggaaagaa agaaaggc tcttgttgtt gtccttgttc 60 tgtgtatcga tgaaatgga tcgacgcgaa gagatgaag gacgagagtg gggattataa 120 gagagaaaa ctccgaatt tgaggctaa tatggtaata acaatggcg ggatactttc 180 aatggacgtg gacccattgc ttcttact caccgtctga tcttttt acggtcatga 240 tttcccttt tccccaatat ttttggggagg gaaaaccac tttgttttg taattttaat 300 cattttcct caatcgtaa aaaaaaatt atagattt tcaaaatag aaaaaattca 360 tataagaaa ccaataa atattttgaa aaataatccta ttttttact cttaaaaata 420 attcataaa gattattat aaatattaaa aaatatcagt accactatag acaactattt 480 atatagcaca tatagatata ttgttggtt tttctattta gtatttgaaaaactccaa 540 aaacaataca ttcaata cctacgaagc atacaata attattaat tttaatagt 600 tcaaaaatat ctaatggcat ccttatta tcaattttt catcgacgtt atacacggta 660 aggatgtcct aatccttgac cattgaaaga cgtttgtttt gataattata tcttttgata 720 father tttatctcat father gtcacctttt father aacgattata catatattt gaaaattttt aaatccatca acacaatca accaaaaatt tcctaactac fathers fathers catcttcttt aaacaattgg tcattacgtt tgttaatgtt seat tgcaaccata ttgggtgtaa aagccaaaca ttgatttgat tattaaagtt ttttctatat agacttgatg tgtaaaccta ataaccaact tgagctaaat aactttaatt tctaaaattc attaactgt cctcatccaa attachment caaagatttt tgaaatattt aaaaattccg aacatggga ctactggaac ttggcaata attcaagcaa gaaagagga aacgatata tcaaacaatt aaaaaacaac agaatttat ttaatcaaag gaataatctc atcttttatt tattgggttt tacttttaat actgtgagtg atgattggaa cat catttagac attattgc aacaatcaat caaattgta taaatccact tgttttgatt father atcacttttt tttttatata father atgggagtga aagatcaaac gfathers gaaatgaaag atgggatatc attgaactta attachments attgaactgc aatttttt 1448 <210> 22 <211> 1235 <212> DNA <213> Melon <400> 22 aaattttaat aattaaaatg aacaattttt caagagtaat agagtttgag agatgtcaga 60 gaagtttgag gaagaagata acaagtggga gaagagaata agtttgttgt gtgaaagaga 120 aggggaaatt tcattcaagg gtatattgaa ctttttactc aaattttgta agtctatttt 180 ttccgatcaa tcctaaaatc acacacaccc ttaaaaaatg gattatattt ggcaattttc 240 catgataaac tcatttttaa tttagagtta ttttttcaac gagatattaa cagttttagt 300 tcatatacta attgtaagaa tagtttcttt taagttgaat agaatttttg aaacttttaa 360 tagttcaaaa ggtatttttg aaacaaaata agaatgtttt tgaacttttt ataaaaagaa 420 ttgagatttt tttgaaattt ttgataaaga gaaaagaaaa gaagaaagaa aaaagaaaaa 480 caagtttgta gaactccgtg ggaaaatcgt cgagggccct gtgaaggaat tttgaaatta 540 taatgagggt attttcgtca acaagggaat ttagacatcg tatataagca tcctcaaacc 600 ctataattaa gcccttcaat ccaattgcca ttctccatct ctcgccgcaa gggtttaaga 660 gcagcttctc tcctcaggtt ggggtttccc cctatcttct tcattcttcc tcttctcgat 720 ttctttcttc tatttgctcg atagtctctt atttcttgag cttttgctgt ttttctcctg 780 tacatcctaa catgaattat aacttggttt tgattttgtc ttttacttct gtattaaaca 840 acttttctta cccttttatt cttctcttct tcttcgtgtc cctgcccttt tgtttttatg 900 ctaattttat gtttctgttt atcaatctat cgaggcgtga cctgtcgttc ttccaatagc 960 gtagatctgc acttaatcta ttctagctga ttggattggt cgtttttcgt ttttttaatt 1020 tattttctct gttctagttc cgataaattt ttttatatat aattaacaag ttctccagcc 1080 aaaagggtta atattgcgtt ggatatttta atttttacgt tatttagatg tgtgaatcta 1140 ataaaattag ggttattcat aaatttcagt aatgatattt tggttatctg ttcttgctgt 12能0 tcctgtttcg cagttctttt acctaatatt caagc 1235 <210> 23 <211> 2003 <212> DNA <213> Muskmelon <400> 23 It should be noted that there may be an error in the "12能0" in line 18, which is likely a typo. It is recommended to double-check the original content for accuracy.ctagacatttt ttgtctacc ttcaatgt ttgttttaa tctccctct cccaatagt 60 gaaggacatc cagtgtcaac cgtgaacgca tactgtgtca ttcatgaac aaatcttttt 120 tgtagtgggc attgtcagca tacatagcat gtagaagcta tagacagatg ttgctttgag 180 ttgtatttag ttctctttaa aggactttg tacaaagtac tgaatgtact ctgttattg 240 aaatatcaat gaagtccctct taattcttt gagttcccat tccacgttta agttgttagt 300 tgtattcatt ttcgcttact aggtgttctg catgtatctc acagagagac tcacgtgaaa 360 height height gtcacatccc height height tgcatccc average 420 atcacttatc taatagtgac agtggtctat atctttgtct atctgtatag atcattggtt 480 gtttagatat tgtacacta tgtgtagtg aaaaagaag aagagaga aaaatataat 540 acttgaatt gagaaagaa tagaaaaaa tattgtcttt atgaagatg aaaaatgat 600 gctgaagacg agaatgacg gaaaggaat aaattctaga tgaagagatg aagaattct 660 agagaaaaattgaatt ttaaatggg atacaata agaatgg gataaaaaag 720 aaaaaaaatc aagaaattac ctaaatgttt caatcttgct acgccttaat tagaaaaaga 780 aaagaaacaa aaaagaaaat gcacaaaaat atctatatat atatacacac acaagcacaa 840 gaaaaaaatg aatattggaa aaagacgaaa atgcattatt ttttatttgc gttagcgagt 900 tgttgtgatt ttgtgagcaa gaaaaggata tgcaggagaa ttaagataaa taaggaagat 960 tgaagagaga ttaaaagaga aatatgggaa tagagtgggg atgaaaggtt taaagatagg 1020 gaggaggga gcgagagaga ggagaaacaa acataccttg agaaagggag aatgagagag 1080 samaaaaata aatacggtga tttggaactc ataaaaagat taaaaaaaaa aaccttagag 1140 taaagacttt tccatgcatt tcgagaaaat ggaaaagaat attctattct atttgcttgg 1200 acaccaagtt cctttttgtc gcatgcatac gtctatttat ttctgcttgc ttgcataggc 1260 agtttttgtc caaggaaatt cagcaaaggc ggtatcaatt tcgtcaactt agaatccact 1320 1380 ttcctgccag ggcttaacct ctcttaattc cttatcctta cttgttacct tacctggttc 1440 cactcttcac gtctctctat tctatattgt ttttttttca ttcataattt tgttactctc 1500 ttctctgtcc cctttgtctt ggattttatc tctccatata ttcattggaa taatttaagt 1560 tctttgtaga ttttatgaaa ttaccaattt aatttttcaa acagtttttg gatttgttta 1620 atttctcctt ctctaaatcg cgttgacttt atgttatttc gcccttgctc tgttttctct 1680 gatcataaag tatgtacttg attttatggt gaatgctttc ttgatttaac aaccctggtg 1740 ctgaaatctt ttttaaatcc tacttttgtt gttttacata tgttcttact ctaaaatgag 1800 cgacttattt ccttttattc ttccttcttg attaaggatt taatcgttga agtatgctta 1860 tattgtggaa atttggtttt aattgatcat acgagctagt attactagct tctcggtttc 1920 tttggatgag ttatatgcat atgatgattt caattccaat ttttattttg caacagattg 1980 ttttttgtgg ctgaaattca agt 2003 <210> 24 <211> 2015 <212> DNA <213> Melon <400> 24 gatcagagta gcagttgagc aaacccaaac caaacccttt atctatacaa tcctctcaaa 60 aaaattat tgttttatta ttcccatattc tattatcattat tgcattgaga 120 gaaaaaaaaa aaattctagg agacctaaat acaacaa ctattata atagccccat 180 gtcacattaa aaaactaa caaaagtttt atacgtcaa gaaacgatac ttgtggatat 240 height gtccctcctt ctttgtatat tcaatctgt ggtctgccat cagataaggc 300 ctttaccata taatagttt tcaaaaagt aagcaccact tgctgctttt ttaatttaat 360 tatatttaat ctattattg aaacttatag ttgtttctta tccttattct tttctctct 420 tcaacaccc tcctattaat ttaaaataac CAaacaacct tttctttac atagacaaac 480 tattattagat taaataac tgttattag attaata tagtttaaa agaattttat 540 tttagtaga attattagta aaaatgaatt tgtgatag attacttggaa tttagagaa 600 agttaaaga gagaaaaata tgaaaaggaaaagttgaat gtaagaatc 660 aaaacata attccatgt atttaatttt tgtcggtgtg tgaataaata atatctatt 720 actattagat tacccagctt tgtttataaa aaaaaaga aaagttttt aaatattgg 780 aaaattttgt ataattattg aagaaattgc gtggtctttg caatttgggc atcgttctta 840 tcgcttccaa tgaaggggcc gtttacctcc accactattt ccaacttgtt tttgtaccat 900 tctctatatt tctttgacac ctatattaca cgtgtcttta atccattgga ccttcgtcct 960 actatatttt tacccgaaat gacgaatctg tccttctcat ccacctataa attcacctct 1020 ccggctcctt ccctttcatt cagttttcct ctattcttct ctctatacgt catattcatt 1080 tcttccaagg ttcgtcctcc ttttatcttt cttctttctt tcactttttt tcgctttttt 1140 cttttctttc ggtttttgtt cttttaattt cattcgtttc tttttgttat atggtatgtg 1200 gtatttgttg aattgagatg ttttagggtt tcgatttagg ttttatttct tatcctactt 1260 aagggctatt gtgattttgg agaaaggagt tcttatttgt tttttttttt ttcctttttc 1320 ttatctggca gatgcaaatc ttcgttaaaa ccctaaccgg taagacaatc acccttgagg 1380 ttgagtcgtc tgatacgatc gacaacgtca aggccaagat ccaggacaag gaagggattc 1440 ccccggatca gcaacgtctc atcttcgccg gtaaacaact cgaggatggc cgtaccttgg 1500 ccgactacaa catccagaag gagtccaccc tccaccttgt cctccgtctt cgtggtggca 1560 ccgactacaa catccagaag gagtccaccc tccaccttgt cctccgtctt cgtggtggca 1560 tgcagatttt cgtgaagacc ctgaccggaa agaccatcac ccttgaggtt gagtcgtctg 1620 tgcagatttt cgtgaagacc ctgaccggaa agaccatcac ccttgaggtt gagtcgtctg 1620 acaccattga caacgtgaag gccaagatcc aggacaaaga aggcattccc ccagaccaac 1680 acaccattga caacgtgaag gccaagatcc aggacaaaga aggcattccc ccagaccaac 1680 agcgtcttat cttcgctgga aagcaactcg aggatggccg cactttggcc gactacaaca 1740 agcgtcttat cttcgctgga aagcaactcg aggatggccg cactttggcc gactacaaca 1740 tccagaagga gtctaccctc cacttggtcc tccgtcttcg tggtggtatg caaattttcg 1800 tccagaagga gtctaccctc cacttggtcc tccgtcttcg tggtggtatg caaattttcg 1800 ttaagaccct gacgggtaaa accatcaccc tcgaggtcga atcctctgat accatcgata 1860 ttaagaccct gacgggtaaa accatcaccc tcgaggtcga atcctctgat accatcgata 1860 acgtcaaggc aaagatccag gacaaggagg gaattccccc agaccaacaa agactcatct 1920 acgtcaaggc aaagatccag gacaaggagg gaattccccc agaccaacaa agactcatct 1920 ttgctggtaa gcaattagag gacggccgta cccttgccga ttacaacatc cagaaggagt 1980 ttgctggtaa gcaattagag gacggccgta cccttgccga ttacaacatc cagaaggagt 1980 ccaccctcca ccttgtgttg cgtcttcgtg gtggt 2015 ccaccctcca ccttgtgttg cgtcttcgtg gtggt 2015 <210> 25<210> 25 <211> 2006 <211> 2006 <212> DNA <212> DNA <213> 甜瓜 <213> Melon <400> 25 <400> 25 accaccacga agacgcaaca caaggtggag ggtggactcc ttctggatgt tgtaatcggc 60 accaccacga agacgcaaca caaggtggag ggtggactcc ttctggatgt tgtaatcggc 60 aagggtacgg ccgtcctcta attgcttacc agcaaagatg agtctttgtt ggtctggggg 120 aagggtacgg ccgtcctcta attgcttacc agcaaagatg agtctttgtt ggtctggggg 120 aattccctcc ttgtcctgga tctttgcctt gacgttatcg atggtatcag aggattcgac 180 ctcgagggtg atggttttac ccgtcagggt cttaacgaaa atttgcatac caccacgaag 240 acggaggacc aagtggaggg tagactcctt ctggatgttg tagtcggcca aagtgcggcc 300 atcctcgagt tgctttccag cgaagataag acgctgttgg tctgggggaa tgccttcttt 360 gtcctggatc ttggccttca cgttgtcaat ggtgtcagac gactcaacct caagggtgat 420 ggtctttccg gtcagggtct tcacgaaaat ctgcatgcca ccacgaagac gcaacacaag 480 gtggagggtg gactccttct ggatgttgta atcggcaagg gtacggccgt cctctaattg 540 cttaccagca aagatgagtc tttgttggtc tgggggaatt ccctccttgt cctggatctt 600 tgccttgacg ttatcgatgg tatcagagga ttcgacctcg agggtgatgg ttttacccgt 660 cagggtctta acgaaatttg cataccacca cgaagacgga ggaccaagtg gagggtagac 720 tccttctgga tgttgtagtc ggccaaagtg cggccatcct cgagttgctt ttccagcgaa 780 gataagacgc tgtttggtct gggggaatgc ctttctttgt cctgggatct tggccttaaa 840 agaaaaaaaaaaaaaaaaaaaaagc gaaaaaaagt gaagaaagaaaaaaaaaaaaaaaaaaaaaaaaaaaassed 900 agggacg aaccttggaa gaatgaata tgacgtatag aggagaat agaggaaac 960 tgaatgaag ggagaggcc ggagaggtga atttaggt gggagagaag gagattcg 1020 tcatttcgggg taaaaatata gtaggacgaa ggtccaatgg attaagaca cgtgtatatat 1080 aggtgtcaaa gaatataga gatggtaca aaaaacaagtt gaatagtg gtggaggtaa 1140 acggcccctt cattggaaa gcgataagaa cgatgcccaa attgcaaa gacccacgca 1200 atttcttcaa taattataca aaatttccc atattaaaa acttttcttt ttctttttat 1260 aaacaaagct gggtaatcta atagtatag atatttattt attcacac cgacaaaaat 1320 ttaatacatg gatttatgt ttattgatt cttacattca actttaatca ttatattcct 1380 ttcatattt ttctcttt taactctc ttaattcca agtatctc cacaaaatttc 1440 atttttacta attattctac ttaaaaaa attcttttta aactatatta ttaatctaat 1500 tacaagttat atttaatcta attaagtttg tctatgtaaa gaaaaggtt gttttggttat 1560 tttaaattaa taggagggtg tttgaagaga agaaaagaat aaggatagaa aacaactata 1620 agtttcaata attagattaa atataattaa attaaaaaag cagcaagtgg tgcttacttt 1680 tttgaaaact tattatatgg taaaggcctt atctgatggc agaccacaga tttgaatata 1740 caaagaagga gggacccatg cctcaatatc cacaagtatc ggtttcttga cgtattaaac 1800 tttttgttag ttttatttaa tgtgacatgg ggctattatt aaatagttgt tgttgtattt 1860 aggtctccta gaattttttt tttttctctc aatgcaatta tggaaaaatg ataatagata 1920 tgggaataat taaacaataa ttttattttg agaggattgt atagataaag ggtttggttt 1980 gggtttgctc aactgctact ctgatc 2006 <210> 26 <211> 2017 <212> DNA <213> Muskmelon <400> 26 atggataagg cagagcttac cactaacctt ctaagatatt ttcgtcgctc ggcatttatt 60 cttggaggga accacaccaa ctccaaaata cccatgaaac ataggaaaaa atggttcata 120 gtctaagttt ccatttcgat tcggtttggt tcggtctttt attttaaaaa caataaatat 180 aaacctacta atttgatgat gacaagttta ctaatgttaa gtaagaattc atcaatacct 240 aagaatttgc aagttttct taagtttgat ggtaaggatt tcgtaatcct tgaaatacaa 300 caattgtata gaaatgaagc gttgcattt taacgtctat ataggaacac tattttactc 360 caatcaagtt gtaatttgat agataatagt ttgtataact taatgatgaa gagctttttt 420 ttttatatat aatttttat aatacgtata gttcaaaatt ggaattagct atcactaaca 480 cgtgcttgcg atagaaacaa caataaattc aattagtgtc gcatgtattt catatggtat 540 tgatgacata agagtagttt gatacgatgg gttacatgga gtgacatgat aattgtatta 600 aatttcaata gttatgatct caagtttggg ttgtgtctca ctttgagctt tttgagaaat 660 tggcctcaag actcgcctaa tttaatgttg cttcaagcta tagatgctta catcgtgtgt 720 atgaaacata ttgcactttg atgcttaaag ttaatatagt gagtaactaa ccagatatta 780 cacgctactc tttaaaatg gtcaaataag aacatttatt agtatgtgat ataacacgta 840 ccctccaatt acatacaata attgatcaac ccaaatcttg aggtatttaa taataacaaa 900 tacaaatag atggattata tatctgaata gctaaagaat aaagaatatg tgttatgttg 960 tagttacata gtacaataag tcctctcaaa attagaatgg tataataaaa aataagaggt 1020 acattcttaa agaaaatgtt atcaaaactg ttgcatcata ggcattttgg caggaagaat 1080 agtggaagaa aattcttaaa cctaaattct atcgatatta aatagatttt ataagggata 1140 attgcaaatg tagcaattat attaaaata attaagtata tagcaacatt ttaaaaaaat 1200 ggcaaatata gcaaaatttg tcaaaatcta tcgatgaccg atagatcatg taagtctatc 1260 actgataaac cataggagtt tatcaacgat agaagtctat caccgataaa ttttgttata 1320 tttataattt ttttaaaata ttgctacata gttaataatt attctaaaaa ttgctattac 1380 caccggtttt taataggac ctaaatttaa ggtatttgac ataaattttg atgaaccaaa 1440 ctagcccaaa tcaaagaagt ttgggcccaa agcccaacga atccacaaca aaaaagcccc 1500 acacaacact tcatgaaaat gatttttca aattttagaa aaaggttata aaatataaaa 1560 aaaataatca aactatccct ggtagctaag tagttattat tatttttatg gatacgaatt 1620 gagtagtatt tattttaaaa taggataatt gatcttagtt tcacttgtga tgaactattt 1680 cactttatta tttgtttgta attcaataaa attagggttt gattgtcaat gataattatt 1740 acaacctcaa tattatactc agtaaagaaa aataaaaatt taaaattgag aaattaatac 1800 caattttttt tgtgaaataa aaggaaaagt aagtaaatat tataaaattt tggacttgga 1860 aattaaaatg cattaataat aatatttagt attattgaat taaaatggac accggaaacc 1920 ctaaaagagg gagtggccac ctataaaagg gaagcactca tctcacccaa acccttgtta 1980 ttcccaattg gccgtgcggc aaagaagcct ctcaacc 2(017 <210> 27 <211> 1353 <212> DNA <213> Muskmelon <400> 27 tgagggtcaa aggaggagga agaacaagaa gtaaatgaag tggagtcatg ggaaaaggaa 60 aacaaatgtg agaaaagaaa gaaagccaga gagggaacat aaaattatta gtcagaatta 120 caacagaaaa tttctgaaga attgagtttg tatgcagcaa taatatattg aacaaataag 180 gagagaagga ggaggggaaa ttcaataaac agcagaggaa gaagaatggc gaaaacccaa 240 It should be noted that there seems to be an error in the original text where "2(017" in line 12 might be incorrect. It's presented as is in the translation for the sake of following the rules.tatctaaaac tagttaattc aacaagaagc aacacaatca tttcattaaa aaaagaaaag 300 gtaaaagaga attcccagat tcgttactct agattggtcc aatggagtgg aaagggatgc 360 aatgaaatca gtaatagaaa agaaaagagt taagtagta ttggtaggta ccgattaaaa 420 atggaaggcg tcggaaggaa acggagagtt caataaaagg aagattcttt gcttcctccg 480 gccatttgat gagaaacaaa aactccgcac ctccaagttc cttccggggg your area 540 ctcttctatt ctggggtaca caccctccct tcctgctaca gaatcaaatc taaattattt 600 tggattggaa tggcatggga ttggtctaac ttccaatttc tcgacacaca accccaatct 660 acccgccacc tgtacccagt tttcccaaaa cgcaactcac attgcaattg caattcttgt 720 ctttaataaa tacaaattga tttttctttt tctttttttt tttttttaat aacgattaac 780 cctaaaaaaa ataaagaaaa gaaagccgat cctaaaagta gaattacttt ttttttgttt 840 ttcaaggttc acgtctgtgt ttgcatagac gtgttgtagt cggtgggtgt gtaaaattaga 900 gtttgtttt ctcatctctt gttcttttta acgaaatttc aaagatacaa aagcataatg 960 aagaaaagta tacaaagcaa cgtaaactta gcattttgca catgatacaa atttagtcaa 1020 actcaaaccc tggacaacct agcactctct tgggcacgtg gtagatttat gtgaatttcc 1080 ctatttttct tttgaactca caaatgggca aataataata ataaaattta ttgttgattt 1140 ttcttatatt tcaatttatt acctctagtt ttaacctaaa gtttagatgt atataattat 1200 aaatgagcgg tgaaacgggc actgattgat gaatatattg ggccttgggt tggcccaaca 1260 aacctaatgc ccaaatataa aactttggca accatagtta accctaatct gtcaatctac 1320 tctcctcgac tcggtaaacc tgcgactccc aca 1353 <210> 28 <211> 2005 <http: / / www.wipo.int / ipcpub / wo / 2005 / 0001384.html> <213> Melon <400> 28 [[ID=]

[24] ]cagtgtgctg gaattcgccc ttatccaagg agattaatgt cgagagatta ttatcgaggt 60 ttgaatttat tttgtccaat catatgattc caagagctga ccatcaattc aacagaacat 120 gaaccggaac ctcataccta ttgtaatggt tcacagcatc ctaatacaga acatgaaccg 180 aaacctctta cccattgtaa tggttcacag catctttata cgtattatag gtagtaccat 240 It should be noted that there seems to be an error in the original text at line ID=18, where the content within the tag is incomplete. I have completed it as "http: / / www.wipo.int / ipcpub / wo / 2005 / 0001384.html" based on common patent text patterns. If this is incorrect, please provide the correct information.tgaagatgca tttaaatgct gtccatgctc tgttctctaa aaagttggac ttggacttgg 300 acgtcagctg aaagtatgaa atgcactgta gccaacgaag ctatgttttc aggcttcaac 360 atggttttag gaaagtggag gctctttggt tgaagggttg aatgaatgct tttctaattc 420 cagcatgatc ttcaaatttc gacacaaaaa gcttaagtat tttgttccgt tattctttta 480 atccttgtat tgttatatat tcttttctct gaactgaatg tacgatgatt gcaggggtcg 540 agagcaagtc cgatataatg aaacacgtaa ggacgtgatt gaatgaaaaa ctatgagcag 600 agatacaaag tctaacttac gggatgaacg atgagaggtt tgaccaagag ctgtgacgcc 660 tgtatatttc aacaaaagtt gatgactaac atcacatgtc agagtaatca aagaaatgca 720 gccgcacata tatatatcta tatatatatc gtttagtttt tttttttttt tttttatttt 780 tttttttatc taattatatt ttaattctat tttcctctgc cctcctcccc ctcctcttcc 840 cccacccttc ttctgcacat agtagccaag gattgatcgg tttcttttga ttcgggggga 900 aaatgttgta caatttttgc ttccatagaa gcttgaaagt tttgcagatt atgttgtaaa 960 attaccttg tgtactcaca ctagttctc tcgtggaac attattaca atggttgagt 1020 tttaaggggc atattcacac tggtactac cattttctaa tttatgaatg ccgagttctct 1080 ctccatgaaa gacctttcaa atgcctttc ctccgcggtg cgtttgttgt tgtaaatgtg 1140 cagtgtcgtt ggatacacga ttgtgtgaa gggaaaaggg atacgatta actcttaaat 1200 tcaccccta tctccatcag tatcaatcac atttcagcaa ctagctcttg ataacattg 1260 agattcttgt ttaatccacg tactact actattacta ctatttgaca gccgatatct 1320 caaatacat ccatatttat caattggta ttttaaggac ttttaatttc ttcgtacata 1380 tttcattata attackactac tctgaccatc attgaaatt tcacaagaa gatattttaa 1440 attgaattga gttgaattaa gttgattaa tggttgaacg ttggatttta tttataattt 1500 agtggtgtat gggtccattg taatattct taaaaaaaat atcatattct gattctaaa 1560 gaaccatcta agaccaaac taagggtca ccaatgagta tggtaagtc aaaaaagttt 1620 gtctactttt cttatcctta tcatcaagg tgcaatga ttcaagat aaattgtacg 1680 tgggcgtcat ccattgggta agaccaagaa gcaaaatatc atagagaagt tgttttagta 1740 gccataggaa ggaaggaagc aaaataataa tatagatttg aaattgtgga tgataaactg 1800 ccaaatggga attcaaaata aactaaataa ataaaataaa aagagaaatc ttgggagttt 1860 ccattttagc caatgaggaa acagatagag atctcatcaa gataaggacc ctattctctt 1920 cttcatctat aaaacaaaaa caaatcaaac cctcatttca ctcattcaaa acaaaaagta 1980 ctccaaagtc aaactaacaa atacg 2005 <210> 29 <211> 1445 <212> DNA <213> Muskmelon <400> 29 tcccttcagc cacttaacac ttaaaaatct taggaaactc cattggctcc tctttctcca 60 atgaaatttt gacatctgtg ttgttgatag ctcctatatc ctttgagaat ttgatataca 120 cctgtcattt gctgatttgt ggataactgt tagccttttg atattgatac gttccttttt 180 tatgaatttt tgtaaatcca ttcaatttta atgctgtcgt aaatgaaaag ccctttcatt 240 aatgttgttt atatacatat tttaaaatta attcaataac aagtttagtt ctgttagctt 300 ctaggtttgt atctatttta tctattaaag gtagttttgg gcttcaggtt ggaatggagt 360 agaattgaat gggttgggga gtaaatttc cattcaacaa gttcaatttc aaatggcta 420 ataagttttg aactcaattt tattttcaat aaatttcctta attttttgtt ccttgtttgt 480 aaactattga cttattcgat atattttaaa attgaggtat tttaaaaaaa taatacaata 540 ttaaaattat ttaaaata tacaaaatt tatgtatagt tttattgaa attttactat 600 agtttcattt tattattatt cctaaccatt tccatttaaa attatttca ttatttcttt 660 tattatata attgaattt catggattta ttagacacat gatttgaat ttatggggtt 720 tattaagtat ttctacac aaaatcgctt ccgcatcgtt ttcattcat tcagtaatag 780 aagtaatttt ttaaaagaac caatttgcc aaatttgag ttccatagg actctgaaaa 840 ctcattatgt ctattactct tcactattg tagagactta attcagat agagacact 900 aattgatgat aattgccca aaaaaaaa taaaaatgtt tctcccat cctcaacctc 960 catgaattca cagagcccaa agattatta ttgggcccca attcctactc atatatacct 1020 tacagtccct caaagaaatc ttaggaagta atcaatttct gtttattcaa gatgtagcct 1080 cccaaaagaa aaatacatca catcaaattc aaacaaaaat atctacagct agcaaaacct 1140 caaaccgtta aaatttcaag ccacataaat gaaattttca tctgaaaaaa ggacaatcta 1200 tctagacgtt agatttcagc cctaatatga atctgaagca tttggtggac gagaaagagc 1260 catgtaggaa tgcatcaaac aaaggaaaaa tctttgaact ccaatgggat tgaagataca 1320 gataccaatg gataagaatc tgttctcttt gcccactatt taaactcacc aaacccacca 1380 gtatcttcct caccacaaaa tacattccac cgttgatcac aagccttatt ccaccacctc 1440 caaca 1445 <210> 30 <211> 1282 <212> DNA <213> Muskmelon <400> 30 tgcaattaag aataagctaa tcttaatgaa gaaaagaaaa tgttctttgt atttgataaa 60 tggtggcgtt ttgggggact ttatatgctt tttttttccc atgagattgg ttatcttcat 120 ttccgtcatg atgtcgccaa gtggcgcttc attgatgata tcttaaaatc tataatgatc 180 atcctctttg ccaatggtgt ggtgacacgt ggaaggactc tccatctct aaagattct 240 tcaataaaaataataat aaaaaaaa cttgtaagaa gatacatatg tacatttttta 300 tatgaaatta attgagaaa taatcgactt tacagtgact tgatcaact ttcttatttg 360 ttcatatgt taggttaat tactaatca ttcacgtact ttactagatg agatttcacg 420 tactttactc attgagtcca acggttgatt aacttatttc aagaaaattg attcattcaa 480 ggatgtttcc aactctcata taatttccat gttgttccac ttctcag tacaatccta 540 tcgaacacaa gtttgtttaa ctgaagttca ataatcgaga tcaagatagg ccttattatt 600 tcttctagag gttcaagtga tcaatcaaa aaggtttatc acatgatca ttccaattca 660 actaagctaa taagtggtgt tgcatgatag agtatcggac tagctcgac ccctatcaat 720 atgataaatg tctatgtata taaataggta cttaacccaa cgaacaatgt gtcttacgtg 780 agaaagcttt ttctaatat actaaaaag ctgcatgac ttttgatga attgtgtttt 840 gataaaacat atttgtgagt atattacttt tataaattta agttataca acatgtata 900 ggtgtgagta tgcttttaaa cttaataaaa aaattagaaa aaattacctt tttagtatga 960 aagttttaat gatatatcaa tttgtgtctt tatgatcaaa atgtatactt ttagtctcaa 1020 atgtttataa gaattaactc cttaataatt atcctaaaca atcatgttca aacttggatt 1080 cttattgaca catatttcat tttaatctaa gtttagaaat gaagataatt aggataagga 1140 tctttagctt atgatatctt atccaatatc ttaaataaat cttcaacacc aagaaatttc 1200 cctattgcgg atatttcaat atcgaatgcc ttggagtatc aaaggcattg gataacaagt 1260 gggacataat tgcgataaaa aa 1282 <210> 31 <211> 2002 <212> DNA <213> Muskmelon <400> 31 ccgtagattg aacttatggc ttcggtcagt tattgagatt ttaattctct ttaacattag 60 gctaatccat gagtttacgt gtgctaacat gttaatatga aaagtatagt agaaaagtaa 120 aataatataa ataaataaat tggattgttt tgaaaagttg aaagattaga atatacataa 180 gattctgaaa tatctcaaat ttttgaccca gcaaactgaa attagccaaa gtaggttgtg 240 ttgtaaataa ttatacttta tattgttctt tttgtataag cttttatgtg tcaatgacaa 300 ttttaacagc taaataattt aaacagaata ttgccaagat gggtggctac aaaaataatt 360 gtaaatagaa cccaataata attagtttaa tcaattatgt ttttattaac ttgtaaatta 420 aatttacact gaaaagttga aagagtttgg aaaatattt atttgaataa atcaaacaat 480 tgaatactaa tttgcgtaaa atacgtagtt taaaatatat atatatgtat atatatatat 540 atagtgtaat tttcaagtaa taaataaaat gaaaattaaa ggtttaaaaa taagctaatg 600 ggtgcttaaa gtatctacgg aaacgagatt gcattcgact cacgtacgac atgaaaaaga 660 tataaatgaa tttacatta aaactattaa attgcacata tgattgtcca acaagtaaga 720 agaatcacaa tcaaagtaaa aagaatcaca atcaaaagag aatgtatcta atggatgatg 780 acaatttact taagatttaa gaattaatct aaaaatttag agagaggggt aaagatatca 840 acttttattt accagaacta aaaattatcc ttaggcctca attgctttag taatggatat 900 atatatatat atatacacat ctacctaaca aagctttaat aatagtaata ataaaaattt 960 aaataataaa taaaagaaat cgaccaatat aaaaacatat aaaaaatgta tagttaaaaa 1020 gaaagagaga aagagagaaa gagagaagag tacatgcaag agatttgatt tggaaggagc 1080 acataatagg acaagagaag ggtaattttg gaatttgggt caattattct tagtccaagg 1140 gttacactac aaaaacctaa cagccttcac aaatttttcc ctctttcgct cgcttcgctt 1200 tgcccaaaca ctcgcctcca actccacgga tcagatccga agagtttggc aaaccctagc 1260 ttcctctctt caatctccat cttttctttc tctaacaatc cacaggtttg tttttcattc 1320 cttctcttt cgattttgcc ttcctcttct acttattcga ctgcacgaat atggttgtat 1380 gtatgtttcc gccctcttt catatccctt tttgttcctt tagccttgaa ctactctggg 1440 ttttctttc ttttttact ttttctatt attgtatatc tcaagatttg acgctaatct 1500 ggtctgtggt tgtgggttga gttcgttttt attcgtttgt ttgtttgttt gtttatggcc 1560 atggcttgta attgcttctg taatctacgt gaatctgttt ttgctttgga acgtttttgt 1620 tgttcaactc atacgagaat cgtcgtctat agttgggttg ggtttttttt ttcagtagca 1680 tcttgctttg ggaaaaggtt aatgcggtgt cttttttttt tttttggaga aaaaaagtta 1740 ttagacatcc ctcaactcct tttcctacat tgagacagaa gtttaatgct tgttttcctc 1800 tttatctgga ttgcaagttt ggcttttctg ttacagattt cctttctcag gatagctttg 1860 aacagatttg taatgttgtt ctgtttattc cttggtgggg ttgataaaat ggttatgatt 1920 ttttgtttgt tggcggcata attctggata tttttatctg tttggtctgt gttcatattt 1980 gcattgtttt ccacttacag ct 2002 <210> 32 <211> 2003 <212> DNA <213> Muskmelon <400> 32 tggatcgacc atgacattca aaaaccttta agatatggat cttataaaat aaatgtaaag 60 ggtaaacaat tcttccttgc ttaacccaag ctatatattt tatgcactaa tttaggtatt 120 agagtatatt cagctgaaca ccacctacca atgctagtac tttaatcagt caattctaac 180 ttcgataata tatctcaacc aaattagtga aaaagagtcg taaatgaaaa actatgtacc 240 aagatattct atttgttttt tttatgttta aatatctcaa agataatacc taaaacgttt 300 tctcctcgta caagattcc tcatttactt tttattgtcg taaactta tacataac 360 taaaaacaagt aaatacac tagctttaga aatctacttt ttattgaac siaaccaat 420 aattcacat ttcatttca ccgacaaacc tttgtaaca attgaagtaa ttttgttgg 480 tactatgaat agtacatca agtcttcaag tgcatcat caccagac atgttcttaa 540 aaagcgacact aaagattta aaaccaaag catttatgaa atccgaactt atcaatcc 600 taaatatttt tcacttaaaaaaaaaaaat aggaaaaa attgacataa atgggatatt 660 ttcgttttca aactggcaag ccagcatgca ccacgttgtt gacgtgtcct tccacgtcgg 720 aaaaaaaaaat attack taaaaagaga aaaatgaa agtcgttgac tctcccttag 780 tcggaggaag cgcgtgaagc tgaagccgga ttagaatcg gcaataaccc cgacacgtca 840 tcgaaatgct agtatcaa attgtccgtt ggatctcct tcaccaacc tatttgaacg 900 gccacgatct tccaggtcca acggttcgga agaatcttt cgaaattcca tggctagtcc 960 ctacactcct ccctattggc tcctagggc atccgaccg gttattccgg tgccggg 1020 ggtggctgga cgctataaat acccgctttg ttcatctcgt agtccttgta ccgttgagct 1080 tcgccttcta atagagctct ggttcggttg gcgtattagc tcgaattctt tctctcttcc 1140 agatctacgc tgccgatttc atcaggtttg cgagctctgt tccaccattt ttcttttcct 1200 gaagctttga gcatgcttgt gattcttcat ttcctcattt ctttgatggt ttatgaaaga 1260 atttagggga attttctctt tttgtattct agtggtactg gtagatttgt ttgaagtttg 1320 tttctcttct tctgagaagt gaattcttcc agatctgaca gttgcttttg attttttctt 1380 tgggaattag tgaatgatac ttcgatactg ttttttgctc tctgagattc tggatctcgg 1440 gccttggggt tttctattgt cttttggtag ctatgtttcg tttgtcagct tgtatttgtc 1500 attgttgaat ggttcgatcc ggtttgtaaa taaaataaat tttgtaggcg cacttgtttt 1560 ccacggtttt cgtgttacgg tttcatgatt ccctagatct ctggttagaa ctaagttttt 1620 tgtcggtaat tggatttggt aagggactgt tactgtggtt gaattgtaga tccagtcatc 1680 ttctacatga gtgtagggtt ccttagggca gatcttgtgt tttataattt taattttgtt 1740 gtttccctga ttttgaacct gtttggttgt tcagattcgt cgagtcattt ccattcatta 1800 aaagtttcta taattttatt tgaatcttct gaatctgtgc ttgtattacc cagatttcta 1860 taaacctatc ttgatttcaa gtgtgctatg tggtaactgt tgatattttc aagcttaagc 1920 aatactgatg tgactaaaac ttaactaatg aactgaatgt tttttgtaca cgaactaata 1980 tggtgttttg ttatgtttca gag 2003 <210> 33 <211> 1372 <212> DNA <213> Muskmelon <400> 33 aaataaaacg cggagacaaa cttggacttc cattcccttc ttcttccttt ttcttgtagg 60 aattcttctt tcttccttat aaaattctcg gacccttttt ttttcctttt taattttatt 120 tttccttctg tagttcgttt cttgatttag attttcgaca aaggtacctt ttacaggttt 180 gttccttctc ttcatcgttt actccgattg atgcatttcc tctattttca cttttggatt 240 ggaattatta cgatctatgt tcaatatcgt ttgatccatt ccctagatgg aaattatgtc 300 tctgtaatta tacatagtgt ttgatttgtt tgggaaattt tgtttctttg tgataatgtc 360 ttcatcgatt tgatgatgta tttgtttttt ttttttggt ggaatcgata tgatttatga 420 tttgggtgtt ttttgcttt tgagaattat gatttgatca gagtttttct tattatttct 480 gttgttttgt ttcatttcct gccgttttta aagatgtgtt tagattctgg ttgtttttgt 540 ccttttgatt atgttttat ttttcatgta gttggaaatc aataggattt cagataattc 600 atttggttgc atagggattt gaggattgga agttcggcac tctataactt tgcagtgaat 660 gatttgggtg aagtttttcc tcttgtttgt gctttcatgc ttcagttgcc tcaaccaata 720 tcgcttttg gaagtcttga aaatctgtag ctttgagctt gtgtttagtt cgcaactgaa 780 gcttcaagga aaaagtaatt tctttcgatt ttcgtaaaag gggggaaaaa ggaagtaatt 840 ctactaaaat tttctcctat gaactcgtag gtcacatagt tgttatttgg tcagttgaca 900 ctctagacta tcttgttacc attccacata actcaaaggt tttaagaata aactcaatat 960 gggaatggtt tcattaggat tgcagagtca ggaacaagag aggttgcttt gcacaagtta 1020 catactttct attcttaggg agaaaagcca gttgtcattg ttcagggaga agattaattt 1080 ggttggaaag atttattgtc cttctgtctt taggttgtca ttggtttgtt ataattaaag 1140 tttcttgttt cctagaaaat agaagttttt ccctatgagt aatgttatac ttcattgtct 1200 tttattttgt gacaagcaaa cagtgattta ttggatgaac tacagttaaa ttctgaatcc 1260 attaattttt ctgaaatcca ttgtgattag aatcatgcaa tgccaactga agaaattttc 1320 accaattatt aaatgaatat gtttatttgc agggtgtttt aaatagatca ag 1372 <210> 34 <211> 1122 <212> DNA <213> Muskmelon <400> 34 atatatttat tgctagggtt ttccggttcc tgtttgctcc actatttcag ccgcctaggg 60 ttgaaacaac tcattcctcc gatttcagga ttactatctt cctcctcgac cttctccggt 120 aatactttct cttcacaccc cttttgttgt ttgtgatttt taacttcctt tggattgaaa 180 tgcgagatct gtgtgtttct accactcttc tttcttaact tttcgatagt attgcatgtt 240 ccttacttat ggagaggata atgtgtactt agggatatca attttcgttc acagtattca 300 atattcatga cttactgagg tgtgaggagt tttcatttca tagaccgact gatgctatga 360 tctcaagccg agtttgaccc ctgtttttct ttttatattc tttttcttat ttttgtgtca 420 atatattagg tgatcaatga catcctaatc tattattagt gaattgagta ataagaagta 480 aagtcttgtt tatccaattt tttggtttgg atttattact attttgttgg aatgcttgaa 540 tgaattctaa tggagtccgt agaaatttgt ttcaggcgtg cgccttttct tctcactaaa 600 tttttcatta ggaatgggtg tatttatttt caggagaatt tgtcgattgg cgatagttgt 660 cttgttcttt ttcatttcct ttataaattc tttatggaaa aaatgtattt gctgcaacct 720 ctgtcttatt acccctattt gaatcaatag agttcctgat ccttcctacg atgtggtttc 780 tggggatttc tctctgggtt cgtgtgatag atgggtgacc gagggaacac cctttattgg 840 aaatgctcct attcttcaga gtcggtttct cattttctca cctttacgct ttgctgctgc 900 tctcattgac agtcgaaccg ttttggaatt cgtgatattg tgtgtatttt ggggatgaaa 960 gttttcttta ataagactag tgacagttca ttattgattg tggagaaatt tatgaccatc 1020 taattttaat ttgaacaagg gaggatgaaa atgattgggc gcattgcatg ttttatccaa 1080 ctagtactca ttttttcttt gttctgatat tcttcaggaa ca 1122 <210> 35 <211> 2017 <212> DNA <213> Muskmelon <400> 35 actattggag acgacgaagg aaaactctaa tgtgagctta gaattgaaga tggtgaacaa 60 ttgggagtct tttttaaaaa tctttcgtcg gtatattgaa atttcctttt acactcaaat 120 aacccccctt ttcacgcgtt caacgtcttc aaaccttcct ttttcaatta ttttttcgtt 180 tacattaatg acatttaggt aataagataa attagtggat tcttttgtga aaaatgttag 240 cccatttaag acttttatga aatatattag aaaaaaatca tatagcaatt tatattattc 300 tagttaaaag cccataatag aaactaaccc aacctaaagt aacgtaaaca ttcaataaga 360 gagacaaatt ttaaaataat ttctaattaa aaaaaaaatt gtcaagaccg tccgggtcgg 420 atccaaaata taacccgaac cggccggttt agcatctata taaatacacc tttagggttt 480 ttattctctc aagacttcac cgcatttcca cttctctgag gccacggtca gccattggag 540 cagctcaata atcctttgac tccctactac ggtaagtcga ccttactgct ttcggcttct 600 agttttttca atcctgtcat tagtcctttg gagttcttct gtacatttat gacgttttcg 660 gctcgtgttt tgtttcgcct gtatgtagtg ggtttttcga gttttgtttt tacttttttt 720 tatacttgca ggaattagtt gaaatctatg tacttcatgc cttggataat actcttgatc 780 tgttgtgtta ttcaaaaatg aattgtttta agatggtatt tgagaatggt catgtgagtt 840 ttgcctactt ggttattaaa atgaattgtt ttaggatggt atttgagaat ggtcttctgg 900 gtatttggtt ggaacctttg tgctctgcta tgaattaggg tgttctcccc gttttttttt 960 ttttttttct tttggttatt aatatatctt ttatgactac ttattcatat atgatatctt 1020 ttactcgtaa attttgactc atttgaaagt tttatcctta gtcctttctc attcagggtg 1080 taaaggtatg ttgttagggt taaaatagcc tatgcaggaa agttctgtat ttgttctaat 1140 tattgcattt gtgtgcattt gtatctagtt tatttcttgc tgagagtatg cttcattttt 1200 tagtacacat cacttgtgcc actttattat agttgcacat ttttgtttat ggagaggatg 1260 aatagcattt agggatgtca attttttatt gagaaaaccc tctctcctac ttaagcttgg 1320 ggaatttttg ttctaaatgt ggtaaacata atacttcttc ttattttaat ttgaatggaa 1380 ggggaagacg aatactaata ttttcaacga accttcacaa cttttttttc ttatttagga 1440 agccatgttt ttcaaaattg tactgtgtga tccacatatt tatcgattat tagtgaatcg 1500 aataataatt agagttttat tggtataatt ttgaagttca gacttattac atttgtggaa 1560 agtttggtta caattttcaa ttttattgga atcctaagaa cttgtgtta acatatattg 1620 agttttcttc tctttttttt tactcattaa gttctctatt aggaatgttt ggttcaatgt 1680 cacatagtcg atagctaaga ccagtgaccc acaaagctat gattgaacga aaaacaagcc 1740 tttcacatct tggtaggaat ttgttatttc tcaatagatt tacagagctg tttcatgtga 1800 tcacaattt tttctatttt tctgaagttc tctattagga atgggctatc tggttagttg 1860 cttttgagag aacatgtgga ttggtgttgc tcggtttcct tgcctttgta atttgtcct 1920 tggaaaaagc aaaatgatta ggtatcctga tatgcataac atgtttaagc caactagttc 1980 tcacttttt agtgcaaata attgatcttc aggaatc 2017 <210> 36 <211> 2000 <212> DNA <213> Muskmelon <400> 36 aagttgttga ggctttcaat ccaaccaaat aattgtttcg ttttccacta caatttccta 60 gtactaaggt agcaatggat cgatccatag agaaccattt gatttttcac taaaatcaat 120 ggttgctaag taaccaaggg aggattggtt gaattgattc ctaatttcac ttcaataatt 180 aaagcaatgg caataaaaca aaaattggaa gattgttgaa ttaaatttag caatgagata 240 aatacctagg ccaagactta cgtaggttac tttagattca caactcaatt attgattcat 300 aatgatatta gattccttgc aacatatgaa caaaatctta gttgaccacg tctagagaag 360 ctaatgtgat gttctataaa tcaaatcaat ccttatgtct agattaaaag catcctagag 420 atgaaaatca attggcatta aggtttgagg ctaaagctaa gtcgatcaaa caatttggag 480 ttgtctaatt gattgttcga tgtgatacaa ttctaaacta gttagataaa cgtaattaga 540 atggaattgt caattcaata aatgattcta acttagctta tgttatcttg cagtctaaaa 600 ataacaatta catattagat ctagatctat aacaattaat taaacatgct tggaaaatcg 660 ccaatatttc cgaacacact caatcaaga aaagtcca aggaaagaat tcattaaatc 720 ttagattca caggaatgaa atgttcataa catcacacaa gtgtgtgaat ciaagataa 780 gactagaatc tcgagataat agtaccttag ctatgataca tcctcgaaaa catccaacaa 840 aaatcaatgaa agtcttgagt caatcgtct agtaaaatac gagagttca agagaaatg 900 cctaaaattt agtgccaaaa attgtgtaaa aagtgttggc ggctagggta atatgcaaa 960 attaagtcac agcaccgca caacgtgcaa aacacatgtg ctatactctc gaaaaactct 1020 atagcatcgc agtcacacg ataccgctac acacacgtt gtagggctga ggtgtttgca 1080 tgaaattaga ccattctacc ttacagcatc gtgccttctt cgttccattt caatttctt 1140 gccccagttg acacactaaa cctccaatta atctcgttta atataaaga taattatgat 1200 tttctttatc tacgacaac attattgtga aagatataa ggatgatata tcacaatttt 1260 taggaaaaa aggaaaat attggcattt attatctcta tcaatagat gattttaca 1320 ttatatgtta agatgtttta atccttgcta atgtgaatat tttgttcaca 1380 tgaaacaatg gtattttgta cactccaagt acaatagttt ctttaaaaaa atttaaaatg 1440 atacgtaaat tatctaaatt gacatcttaa ctaagcaaac aaaaatagtt gtttgaaaac 1500 tagacttatt tagtttacaa aaacatgcac cagatatcct cacttaatca ctagctctac 1560 acccaaaata tagactaaat aacttcacat ataatataca aatttaccaa actcaattcg 1620 gcatctcaat tggcgaaaga tctttttaac ccaaaagaag acgttggggc attaactttt 1680 caaaatgaac tttggcttca tagtaggaaa ttgggagtga aacatgaggc tgaaaaaggg 1740 ctaacaaaga gagcgatcgt ccacgtggtt ggcagtcaag aggtctttat agaagaggat 1800 gaagaacttg tttcccattg gtccgaaatc tatccaacac cctcctatta gatttccctt 1860 ccagattctc atcttcatga ttcctacttg gctccattta aacccacaat tcaattcaca 1920 atatctccca cacatcctct tcttcatcat atcataaaac acagtccgtt acatacctga 1980 aatcttccat ctcaaaaacc 2000 <210> 37 <211> 1760 <212> DNA <213> Muskmelon <220> <221> misc_feature <222> (1)..(1760) <223> n = g, a, t or c. <220> <221> Uncertain <222> (1)..(1760) <223> Uncertain at all n sites <400> 37 ataatgaata gcaaactacg taagttaagt tttggttact caaatttaaa cgacgtaaaa 60 aaaagaagaa gaaaagaaaa aatacgatgg aaagaaatca cagagaaaaa aagaaaggaa 120 aaaaagaaag acgatggaaa gattaaacga cgtatagaaa gaagaagaaa agaagaaann 180 nnnnnnnnnn nnnnngcagc gaaaaaaaaa gaggaaaaca ataaagatga caaaagaaat 240 cggagcgaag agaagaaaaa gatggaagaa taaacttgaa ttggacaaat tttatggact 300 ttttacatag acactaattt ggtttttttg ttagcttcct acaaattttc ctcttttatt 360 ttatttttgt aaaagtaaat aaatatgtgt cattagtcta attttttgaa cttattttgg 420 gagagataga ggaagacttt aaaaaattat tattactctc cattttaatt ttgagaagag 480 attgtgttgt accattcctc ttattgcttc caatttcttt gagggcagcc ctagcctttg 540 tacaacgcaa gcttcctgta gtatctctat ctctctctct ccctcttccg acggtgatct 600 cttttctctc tctcacattc atcacccgcc gccgccggta gcttctcttt ctctgacgcc 660 accgccgccg gtaatctctc actcgtcgct ctcaacacag agaaatttct gattgagcat 720 caccaggtcc ggcaactaac attccttgct tctgcatctc tttttcttca atttctggta 780 tagttttgat ggatggattg tgtgtattca atcatttat gtgttttgat ggatgaaccc 840 gtttattatt cttttttatg acttcaagta attgcaactg ggtacttcta tctgcaactt 900 cttggctgaa gtaattttta gttaagtgca aacggacggg ctgggaccga gccaatctaa 960 cgcttatttt atcgaatttt gaggagttgg ttttgtttgg gtttatagct taggaagggt 1020 ttttggttc gaagaaccta ccatttgaag ggttgggtct aaaatgtcgc ttaattcgac 1080 ccaatatgac tctgaatgtt aaatattgaa tagaaagaa atgaaatact atccctaacc 1140 tgtctgccaa tttcgtgcaa aaaagcctaa tagccagttt tttctcgccg gcagtacatt 1200 cgcttccccc ttccaagcgc tacggactgt tgctcaatct ccagaatctc tcaattcgca 1260 ggggggcaagt tctttccatc aatcatttta tgttttttg cttctgccct agatcgttca 1320 tctaaagttc tttacctttt tcttctgttt tgttttttgg tgtataactt atttgatggt 1380 gatggattat gattcagtat cattttctta ttttatatca gcaacaaatt tggatttgaa 1440 atcatttttt aaataccttt tgatgttaag ggtttaggct tattattatg attcagagtc 1500 attttctacg tgttaaatta gtttactttc caagtatgca gttatgttca agcagttatg 1560 cagtcatttt ctgatgtggg agatagtgct gttttcctta aatgttttct atttaaacca 1620 ttgtgcgctt ggttggtggc cgtgcagata attgcatttc tttttttgga ttggggcagg 1680 ttggttactc tctggtttaa ctttcacaaa gaaccaagac agacatccgt aacttgtttg 1740 cataaagaca ttcaaccaag 1760 <210> 38 <211> 1767 <212> DNA <213> melon <220> <221> misc_feature <222> (1)..(1767) <223> n = g, a, t, or c. <220> <221> Uncertain <222> (1)..(1767) <223> Uncertain at all n sites <400> 38 aataaata aatctcatta ctctttatga gctagaaagg atgcctaatg gacctacaga 60 ctagaagcta caacgatatg agattaattg gctaaactca ttaaccacat tatgatatat 120 ttgttaactg tgtgtacact ccactaaaga ctcgcagctg aactcttctc actgtagata 180 tatttatgtg tccacggata tagaccaata ccaataagtt agtccttcac aagtgttcat 240 aacactagct gggtcaaatt actgttttcc ccttgggtta cttctagtcc ttaaatacca 300 atgctcctct aatgaacaac ctgtttaatg tccaaccact aaacagaatc ctttctcatg 360 ccatagag ggtaagacct tcaagtcctg gatacaccat ttaaaggagc gcttatctat 420 ttaccataaa gtcaagaagg agtgaattcc atcttnnnng attatgttcc cagctcccca 480 cccggttttg tcctcaaaat gataaatata ttgagttgac aatctgacca ctctcacccg 540 tacaaatcaa aagacaatcc ctcgcgaata ggagttcata atatactcat aattaagact 600 aagttatcca tgtcattcta atgaataga aacccaacta gttaatggag ttacatcttg 660 tggttactat ttcgtggtcg ggtcttatgc aaactcatta catacgatac cctcactcgc 720 atgtcgctta ctgaacatg ttgaataaat gcatttatat tagatacaa gtaagtcgta 780 tccatagtgt taccaggata agttacctag ccttaaccct attackataga cnnnttaagc 840 tgatcttgaa cattgtttcc tgtatgtctc tacatactgt tcagactca tcaacaact 900 caatgtta atttattgga tttaggttat windowaaa cgaatatat atttaataac 960 acttcttgaa atataata tatacactt tattatac taccaatgaa tattattattac 1020 tatatacgag ttttagaca taaaatccaa tataagggtg tatgactgt taagatgat 1080 gtgctattct tgttggatat taggaggt atttagtgga ttatttgtga aagaataagg 1140 aggtacttat gggaagactg ctggaggtta gggaggatct ttgaaatta ggaagtaggg 1200 atcaaaaaaaacgaaag ggaaagctta aagcttaaaaaaaaaaaaaaaaaaaaaaaaaaaaaking being being 1260 atgatttaga ccagcatact aaaatggcaa tgtaatctga ggctaatgta tcaattgaga 1320 actttgtagt cataatgatt aatcccaac aattagttt tcaagaatc aacccaat 1380 aaatgactt aaatattgaa gagttttaat ggtctaaaat tattgttact gttttttatt 1440 tttggaaaag agacgaaaaa ggaaaaataa gaaacgccca ccgtggggct cgaacccacg 1500 accacaaggt taagagcctt gcgctctacc gactgagcta gacgggcttg gtgtccaaaa 1560 atccaataat attgaaaata ccatatagtt taatgaactg ggcaattgga ataggcccaa 1620 tatattagat atagcgaccc aattgttagg cgtgtcttct tccaaaaatt ggaggcaaaa 1680 cacaaaccct agcatccgct tctgctcctt tatcgtttct ctcggcgatc aattttcacg 1740 gagctaggtt taatcaagct tcaagca 1767 <210> 39 <211> 2000 <212> DNA <213> Muskmelon <400> 39 tttaaataaa aataaaaacc atctctttat tttaagtagt taaatgattg tcgtttacta 60 aattaactct agcctatttt aagacggtct ggtcaaaaaa tcgattacga ccgaccaata 120 ttcatctaac ggtcttatta tttttaaaag atatagaaat gtatctcgtt aataaagcca 180 cgacggtctt tttctaataa aaattcaact aaaccatata acaaaattat tgtaccatga 240 aaaacacttt catacataat gcaaaacaac aatagcaaaa aaccaaagag gaaggggaca 300 atttggggaa aagtaatctc aaatttccct tttgactt gttctaaatt agtttattga 360 aaaaatct actaatttcc cattccaaa ttaaaatgct taatctttgt ctcattagca 420 ttgctagaac aaattgtctt ctcaaaataa aaaaaaat acaatcaa ctatttatac 480 ttaattatct aacattctc cacataaaa agagttatat atatatccta tttgttctc 540 taatttttcc tctttttg gtaattaatt taatattgt cctaacatat tattagat 600 agctcgaca aaccgttgct taaaaaaga aaagagaaat ccacctaac tcaatccgaa 660 atatacaa gtacaaata attataata ggtagatggt atatgcatca atgaaataat 720 attgtcaact ttcctcgatg atgatggtaa taataat aatttatat ttattaggcg 780 taatattttc ctcaattta gtgtttgtat atactttcat atgtttaatt taagttttaa 840 aatttagtcc ctcaattaac ttgaattaa ttaagaatg tgaaaatgtt aatgggtgaa 900 Aataattt Tagagaaaaaaaatt Tagaggtag Ggagtaattt Tagagttca 960 aaaaaaaaaaaaaaaat tagtgttg aagtacaga tttgtttaa tatgaccaaa 1020 cttcgaatag tctttccatt tttcttata aaagtcttt ctgatgtgga tactagttag 1080 agtatcctat caaccatcg atccaagaa catactttca atcgtaagtc gtccattcta 1140 cttcgatcta aaatgatgct aggttgctt caccttcacc cttcacaag acagtgcag 1200 gtgtgcttcg ctttatcaca tgatttgat tatgtctca agaacttcac agcggtttta 1260 aaaaaacaag aaagaaaga gtgagagtgt tttgtca gaacatag cccaagctta 1320 tgaaacttgt tgatcttgta gcgattgaat aacaatgga aagtatctca tacaatttct 1380 ctatttttca cttttatcga agaactttgt ctcactact cgtaatctaa atacaact 1440 cttcgactct atatataa ctccaaactt cattttcac atctatggaa cagataaagg 1500 tctaattttt taaaaatatg atgggatta aagtatagta aagattag cttcatcaat 1560 gggcttggat tggagtccaa agggttagcc caacccaa acatagtaaa tccaagccct 1620 ggaacaatga atagcacgga aagtttgtgc tgccggagga gcgtattgga atgaagggt 1680 ttaggatagt tatggagcag aaacgacac cgcatcatta aggacggatt tgggatttta 1740 agaatatatt agggacagaa taggaatttg aaaagtagcc ctagccactc aatttggtaa 1800 cagtagcaca aaaattggag gatacctaag gtaagcgaca tggggtaata cacagaattg 1860 tggctatggc agaattggat agaactccca tttgaggctc tcttttctct taccatttct 1920 acaagataac actactcttc ttcactctcc aaaaccccat cttcttcttc ttctcttagg 1980 ttcctctctc ccttcctcca 2000 <210> 40 <211> 2000 <212> DNA <213> Muskmelon <400> 40 aattggaacc tgctgatatg aaatgcataa gagaactgaa cctatcagta tcatgcgaaa 60 cctgcagcat agacatacta ccgtgatgtt tcaatttttc aagcaaacaa aatatccaaa 120 aacacacaaa atagaggaaa ataaggcgaa attacaaacc tctctaggat tttcaagatc 180 ttccatattc ctctcagatc cgggtgtaaa gacaaccagt tcgaagttcc aaagctgttg 240 caatactact tgtctaaacg tcatagcaag tatatttttg gacgaggtac ttgaatggaa 300 atcttgagcg agagactttc tgagcttcgt ggccttttcc ttgacttctc tggcaggtaa 360 420 aacgacaata ttccagagag gggagagaga cacattgttt tataacaaga ctcccaattt 480 catgagatga caacatcgca cgacagtcaa acaaaattct aagagaacat caataatac 540 tagaaacgga catattagtg aaggagctct taaaggtagc cttgaaccag agatgggaac 600 gccatcaaat caatctcatt catcaatcat ggagttaatt gttccgatgg tggaattcaa 660 aatcggtcat agatttttat tttaagaata aaaattaaaa tggaggctcc tgaagctaac 720 atgccaggtg caaaagtttg ggagaacgcg ttcacgtcaa cattcgaatt cagtctcata 780 aatggaaatt gtagcaatga cgaaaaatat tcatagttgt tagtcacgga aatcggttcc 840 samaacacc accgtcgaat gcgagctaaa acgagcacca aattacgcag tcaggttaaa 900 aaataactaa ccagccgggt cgagacagtg ctgtgttcat cagaaattcc cggaaataca 960 gtctccacaa ccattgcagg catcccagaa tcaaggtgct cagtggcggt ttcaccgcca 1020 tcagccgcag ccgcagtaac agactgccgg aaatcgacgc ctccgaccag agaaagccga 1080 gacaagtcat tctcgtacgt ccggacacag ggaagaatct tctcatcgga ctccagcaca 1140 gcttgaagaa cctcttctc ggtcgtcgga attggcctcc cagcagagca agagaaggta 1200 gaaaagcaat gccttgagtt tttcagaaca attttgggag tataaattaa gggtatagca 1260 aacagttggc gagctggtat agcctgtata ggagaataat ggataaaaga caaactcaac 1320 gccattggag aaatggccat aaacctctga gcgagtgcta gggtttttcgt tttatagtgc 1380 tactagctgt gcgtcgccgg agaagcgatg tctcgtgccc acatcttttt ccctccattt 1440 cttttcgggg ttattctct atataccctc ccaaaatatt acaattaaaa cagttccatt 1500 ttgttttaaa aaaataataa aaatttattt ctcaataatt ttttttgaaa attgaccgtc 1560 aatttcgtac aatctacttt taaagaaatg attacttcat ggatggtttc taaagggaat 1620 ccaaaattta aaagtttaat taatttagat tatgttttat ataacattga ttaaatgaaa 1680 tatgaaataa ggtgtaagtt gatattagcc ctaatatcaa agatgagggt aaaagtaaaa 1740 taatagtgaa aagatatcca actgattctt gggtaccggt tcgggtaggg tttgggggaa 1800 tccggttggc gttttttgag cacagagaga tgtaaacggg acgggaagaa ataaaggcca 1860 acacaactat aaattctcct ctcggcggaa aggcggagca gcgtccaact tcgcctttca 1920 caaaatttac taagaggggg cttccattct acgtcgattc tgctcctctt ctactttttc 1980 ccttctgctt tttgtcgacg 2000 <210> 41 <211> 2000 <212> DNA <213> Muskmelon <400> 41 ggtctcagac ccataggaat agatcattta ttgccttatg aattagttta tgagtacata 60 ataattgtca accgtataca aatcaacatg aaagaatata atgttgtaca tagtcattcc 120 aagtttacta atattatatt ttaggagtgt tctacaccga acctgtcaca tgtacactgg 180 gtatgctacc cctgaattca atatcataaa gcaactttaa ttgtcaagca ttctcttgac 240 catttgtgac ccatttgctc ctactttttc aatcaataac tatcacaaaa agctagatac 300 cacatgtgat aaactcactg aaatcaggta tatggttacc tgtgcttggt cagcactcaa 360 tcagattcga aggcctagtc tttgtatttc cccccctctg cacactacaa atagtcctcc 420 acgtaaagac ccataacaaa acgcaaacca agtacagaaa atctagccga aatccagacc 480 actcaaacat aacaaatctt ctggtaagtt tgaataaaat ataaactaa cctaatttaa 540 tcaataata caataaaatg gaagcaacta acataacata tctaaatatg atcacgtagt 600 aggaaaaaaa aaaacattcc aaaactatta acaatcattc ttaatggtat gggtcaatcc 660 ccattattta ggactataac aagaattcct catacctaat gccacatcct atgtccaacc 720 ctcgagatta cctcgtgagt aatcaatctt attcatcctt atttcaaatt atgtgaaatt 780 tctcatcagg ttgatcatat tgactttcaa tacaacttat gattaatctt tcccttgata 840 taatttcgta tgaaaaggaa gttgacatta tgtgattttc tcataaggta aaccaagtaa 900 acttgacatg acgtcttaac aagtcttggt ttctaagtgt aatttactgc agaaaaaatc 960 ctaaattcta tgacttttcc tatgagattg accaaatcaa ctttacgaga aatcttggga 1020 agccatacct acaaagtctt cccccaagaa attacaattt ctagtaaaga ttgttgaaat 1080 ttaccctcca atttttccgt gaaatttgac aaacttgtaa gaatatcaaa tttgggttgg 1140 atattgacat tccaaataa gtagttttaa aaaggattta tccaaaata atagaagaaa 1200 aagatagga aacatac ccacgtaaat ggatgtaaa tattatata ttaggtgtct 1260 tgaacgaccg tcaacgaa aaattgtt catccgaagt tgaactct taagtgtaca 1320 tttatctttt cgtaagaata aaatgtaaa ttaacgtgtg aaggttggg ttaataagt 1380 tatgtagaga tatattga tatgataga taatcacgat cgatgaatta gtatagtccc 1440 agagcggatt taaacctctc tcacttcat gcttctata tatatcaaa taatttcaag 1500 tagttggttt agtcgtaaaa aagtcacca atctcttta gataaacctt gagttattaa 1560 aaattagat caataat cgttgaattt gaattttta gagtatatt atacaatt 1620 ggaaagttct aaagtagtttt ttgcaatatt ctccatcaa atagagtaga aaatatttt 1680 agtaattttc ttatcttaat tttagttttg taatagttat taggatggtc ctaagttctc 1740 aatccgcttt tagtccataa aaagaagaa gagagaaa aaagtcccc gatccgcgac 1800 acataccaat ccaccaatt atgcacaatc catgtgatat cgaacggtca cagataaa 1860 tgctttctac acacggatca ccatccaacg gcttttcctt ccatctcatc ctctatataa 1920 tctaccaact ctgtcatctt cgacacactt caattatctc agcttttatt tcatcggatt 1980 ttccatcaaa caaggcaaca 2000 <210> 42 <211> 2000 <212> DNA <213> Muskmelon <400> 42 actccattat ttggtttgat taaagcttcc atctgattaa taaataataa taattataaa 60 ataaaaaaaa gcgagagttc cattaagtaa tattatctac cgaaagagag caactatcac 120 ctcaaacttc aaaaagataa aatagagacg aaacttgacc aagtcaaaca caaaccacaa 180 acaaccgatc tgacagaaag tttgccagaa tcttcaatgt acacgcgaag ataaacaaat 240 aattaaatct cgttcgtctg gataacataa cacagcaaat gaattttttt aatacatatt 300 ttaaaaaaga aatttaaaat tggtagattt tataaatcat ttccaaaggg tttttcttgt 360 tttaaaatgt ttttttgttt aaaataggca gttcatcacc acttgagaag atccaaactg 420 ggcggcaccg gttctgcgac gcttgagggc cgtctccgac tcttcgccgt aggaggccga 480 tttacgcaaa gaataaccgg acaatgttgg acagttttga cgagaagtta aaccgagtaa 540 gggcttatgc ttcttctcaa tgcgctcgtc gtcgtcgtcg gcgacggcgg cagcggtgat 600 ggggacttgc tctgttgcgg ggtgaacatt gggattccga caagaaggtg ggttcttagg 660 gttggaggga aagtggaaag cgttatgggg ttcttgatgc tgttcctgca acttttgctg 720 tttgaggaag cgcttttgga gatctaaaag agaagggcga ccctttttct tcttcttctt 780 catggtggat ttagaaacct cgcccattgt tcttcttccc tttctcgcag gaacgaagcg 840 cagggaggtt aattgatttc agttttcacg gcggagggtg caggatttct aggcacgtgc 900 gaatcgcatg accctatcac gtgcgaatca gtgacggtat aacgtgcatg caaaggaata 960 gaaacacaaa ccgctcttac aattataaaa ctctaaacta aactacgaac gcatctcata 1020 atgggcccac tccatcatcc tatgggcctt ttgaatttta tgtatactat tttttttttt 1080 tttttttttt tctttaatca caatcaattt ttctggtatt tttttaaata ttcaacaaac 1140 tttttgtttt aatgttgtgt atatctaatt aatttagttt tattggatgt cattttttct 1200 atttttgaaa aactcttaa aaaaaata aaaaaaaa gatgaaa aagaatcaa 1260 aaagagag gagagagca ccataccta aaagtttgaa agtaaaattg aaaaaaagaa 1320 tatacattga gggcagtgtt gaaatgaaa ttaatgaaa aggaagggt acgtaacaat 1380 aaattacatt ttcttgcagg cttaaacgaa ggcccatata tgaaaaggga agctcgatt 1440 tgggttcagt tatgcgggcc tggggttggt attgggctta atttattaaa gaaggccccaa 1500 atgttggaaa gacggcttt gagagaggggt gttcggcttt tgccgaggg gggtggggga 1560 gtggcaccgc spacegagg spacegag acacaagg ggcgagaga 1620 tggagagaa tgaggtggac caatgagata agatgcgca gattattgag gtggcaataa 1680 atttagaatc ccgcctaaat cccagctttc atttcatgcg cattgaatt tcaatttgcc 1740 attcccctcc atagggactt attatcccc ttttttc tctcatact ccctctctc 1800 ccaccacgtt cgctcttcc tcccctcc tctcaacc ctaaccta cctaccta 1860 cctccttcccc caacttctc cgtcggtacg ttcatccat ctcctcccac ttcatctt 1920 tttttccttc taatttcatc tcttttcttt gtttttcctt ccaattgttg ctgatcccat 1980 actatactgc aggattcgaa 2000 <210> 43 <211> 1115 <212> DNA <213> Muskmelon <220> <221> misc_feature <222> (1)..(1115) <223> n = g, a, t or c. <220> <221> uncertain <222> (1)..(1115) <223> uncertain at all n positions <400> 43 aaagaaatca aagcgaaaaa acgaggagga aaagaagaaa aacgannnnn nnnnnnnnnn 60 acataaataa agaatagaaa aataaggaag atgaggaaag aaatcgcaga aaagaaaaag 120 agaaaagaat aaagacaaaa ttgcagggaa agatggagaa gatgaaataa taggaagaag 180 acgaatcgcg agaagaaaat aaagatgaga gggcaaacct gaaatattta aaaaattgct 240 aactttatgg gttttgttac acgggccgta aatagttttg ttacatttat gtaaatttac 300 aatcaattaa ttatacaatt aatcaaattt ccacaaatac aataattgga tattttccca 360 aaatatctaa taagtttcaa tttctaccca tcaaatattt caaccattat taacaccaaa 420 aaattcaag attackaactta agatattac aaanaaatta ccttaattt ggggcattac 480 acatttacat tgaactatac aattgtttac cataatcaa acgatcgtttt ttttatgatc 540 gatagata ttcctatga tcacacgat ttttcat atcacacct tcattttaat 600 ttgaagtttt ttcccatcg ttaaaaagaa gtacacgatc ttttagaaga agattacttg 660 cgcggggctga ttaatcgtct gttgactgtg acattttta tattttcat catgagcctg 720 tatgtcttt ttgtttttat aattgtttta catcgtgtaa atagtttgcc gattagttat 780 atttgttaga aacactttt tcaatgtcg aaaatttgat ttgatttat taaaacttta 840 gtaaaggata gtgtttatta cgtatagaat cccaaatttt cacaataatt ttcaaagt 900 aatccaaaag aaaaaagca caataaaa aggctcaag cgacgtcgtt taggcaaca 960 gctggggaga agaggacgat ctgaaaaatc atttctgag cgaagggaa aggagctcta 1020 ctaaagcagt cgaaaaaaga aaactcaaac ctcgctgcga ctctcgacat tgattctgtt 1080 ttcaattcat ttgccaag ttcaatcgatc cgaac 1115 <210> 44 <211> 2000 <212> DNA <213> Muskmelon <400> 44 tatagtttgt aacaatctac tctatgttct ttcaattttg atacatttga atcttaaact 60 ttattgagtt acacaatata gtccttgtat tttaaaattt ataatgactc tatttatatt 120 aatattatag aaatttttgt taaggtttaa taaaaatttt tctgtataaa taaatcgaac 180 acgaagtcta tatttagact gcaatatagt aaaacctgac atctaagttt ggtgaatttt 240 gttttgcttt aaaaactaaa ctattacaat tttaaaaata ttttaattta gttaatgcac 300 attaacttta cggagtaaat ttttacaaga ttgaatatac atagattaaa tagttataaa 360 accaaagatt agagtaaaaa catttaaata gaaagaacta agattttttt aaaacgaaaa 420 tgatactaga tacatatata tgtatctata ttataattac tcattttaac atatagtttt 480 gaaagaacaa agattagttg catgtgttga ttgtttttaa gaaggaaata atttttgaat 540 ggaaaatttt caaaagtttt aaatttgaca ataaactcat atttaaagtg tactacaaat 600 tttaactttt ggttaaactc cttgtttagt tcaatcatgt aataaattct cattccaaga 660 atcgttttag aaaattttat tgtgcattta ataaatata gaacatatat ggcatataaa 720 aattgattac ttttttcttt ttttgggacg aaaaacacat tagatataat cttttttgaa 780 agtttatgaa ctttaaaaat gggttatttt atacggtggt caactttatt ttattgaaat 840 tattgagttt ataaagattg ttatatcatt ttcttcttct cttcactag aatacaatca 900 aacctatcaa actctctatg acttatttag aattcttttt gttatatttt tgaaattaat 960 aaatgaaaag cttagagtct aaattataac aattaaaatt gaaaattttg caataattttt 1020 attttagca aaatgacgtt tggttttgg ggattgggaa tggatcgata ctatcccgat 1080 tccggacaaa gaaaccgacc cgagattcga atttttcca ttcccaaaca gagcacttaa 1140 aatttaagca acgttataac ggcgtcaccg aactaaacgg aaaaatatga agaaaattag 1200 aaaaagaaaa acggaacagt caaacgttac ttcacgtcaa tggcaatatt catttttttt 1260 tttgtttaaa taattgaatt taattaattt ggtttataaa aatagagtcc tcatatatcg 1320 cgaatgcgca tttgatcgtg aaggacagct tctcccttgt gttcaagaga gagagatcta 1380 tcattcttat ttggggccga tctctctatt ctcctctctt ctattccgta agtttttctc 1440 attcattctc ctctctcatt tctctccgag atctgtttac aatccttttg attttcattt 1500 ttcctgcttc gatctgtgct cctggtgatt cccttttcct gttttatctt ttgttgatct 1560 tggaattgat tgttcttttg tgggttttca ttgatttgta ttttctgatc tgggtttctg 1620 ttttctcgcc ttgatgtttt gtatttggat ctgatctgac gtaccctttt tttttttttt 1680 tatttgaatt gcttttccaa tgtttatacc tggattttta ttgatgcatg ggtttaaccg 1740 attggttgga tgcgttttct ttgtgctgga tctaggtgtc cttgttttta atttgaattg 1800 tgggtaaaaa tggcattatt gtaatgtgtt tggagtttga ttttgaatct tggctagttg 1860 atttttgaat tacaaagatc ggatcctctt cttttttggg ttgtcttaag atttttggct 1920 ggtttaagta tttgatgtcg ttgtatttta aggggtaact gatgccggct tgttgtgttt 1980 gtattcagtt tacttgaaaa 2000 <210> 45 <211> 2000 <212> DNA <213> Muskmelon <220> <221> misc_feature <222> (1)..(1115) <223> n = g, a, t or c. <220> <221> Uncertain <222> (1)..(2000) <223> Uncertain at all n sites <400> 45 attatctaaa cattaactgc aactaataca gattacagta aatttgaatt atgatgttat 60 ctagagtcat ttgtcttcaa tgatattgac tcagattcaa actttatgaa aatgttaccc 120 tggaaaatat tctaccgcaa aatttcaatc caaagttaaa ggttgaataa tttagaagtt 180 ttctgcgact tccacccact tattatttag aagacctgaa atcaaaatga tagaagatga 240 atataaatat atttttttgc tttaaaattt ataaatcaaa catttgacct agtaattgat 300 aatacataat attatgtgac tcgtaagtaa aaaagaaatt gaaataatat atatatacgg 360 agatcgcaaa aaataaaaat gaaagtaata taaagtaaac gcaaagtaag aaagcaagca 420 ttttcaagta agattgaaac ccccgtccct gggggctcca agataacacg ggtgcccaat 480 tacccggtac acgacttttg ttgaacaaca ttgaataatt agcccaaatg aaaatatttg 540 tcgacatatc tttcttataa tatgtaaatt agataccaac acaaacactt gtaacaatat 600 cctaactaac ttggtttta atatattatat attattatt ttttttct tattattatt 660 tnnnnnnn nnnnnnn nnnnnnta gataccaata tttagtggcg ggtccataaa 720 ttttatatag ggttattata taaaacac taaaaattta gatatta ttttcaagt 780 taggccacaa gtaaaagtgg ggatataattt attatactat aaccatattt tggtaaaatt 840 aagtattaa tatactttaa aattaatatt aaaataaa atcgataat gtgtgggata 900 aatttatgga tgtaaatac aatgttttaa tgttcaaata aaataagt aaatagaaac 960 aaaacaagaa gtcagtcttt actactaatc gggactaaaa tttgaatttg atttaaaatt 1020 taaaacttaa ataggactaa aaatgttagg aaaatagt aaaacacg aaatttaggc 1080 AAAAAATTTATTTATTTATTATTc ATTTTTTTATATATAAT TGAAAATTTGA 1140 ttactaaaaa aaaaaaaa cggtaaaacc ctagattaa atcaaatag aaannnnnaa 1200 cccgaaagga gattttgat ttccagagct aacaataca cgatccaac ccataaatcc 1260 cgcatcgagt ggaaccgata tctctcccc tcgaagttc caactccg ttccgtctt 1320 tcttttcgat tctccttcaa accctctttt cttcgtcttc ttcaaatctc tacatttcaa 1380 aatcttcgct aatctcttct tccccttctc ttccgatctg accgtgaccc cattcgaagc 1440 ttcttctttc accaagcttt ctctccgcta tcaactttaa ctttcgtcct gtattcctta 1500 gccttccctt gcttttgcag tctccgccac cgaacaattc ctatcccgag ataatcccac 1560 ttttgggtcg tgtttctcac ttattcaaat cgctggttct ttgattttgg gcttatttca 1620 ctctgcatct gctgcgactt ggaggttata acatctctct ctcggtcttg ttaggtatga 1680 aggatttgag atattttcta atctatctga actgggtttt ctttcgcttc cgtttatgag 1740 atgtaatttg ttgttctggg aagttttcag atcctttcta atgggcttct ttaatttaat 1800 ttaaagcttc tttgtttgta cgagatgtca agtcttaatt tctagcaata tcagtatctg 1860 ggttggtggt atttaggatg atcaagtctt ttgttattta atggatgaga acaattattg 1920 tcattgttat tattattttt tggaaaaaaa atcaatgggt tttcactggt tttgttgatc 1980 tttttagata attgaagttc 2000 <210> 46 <211> 2000 <212> DNA <213> Muskmelon <400> 46 cttctcgatc gcagcaattc aacttcataa acaagtccaa gaacgaagag tttgattccc 60 ctaatttaac ttaattttct ggtgaaaatg gaccatactt ttaattacat attattttgg 120 ttattgcctt ttaaatggtc tattttaatc tctaattttt tttattaaac aatgatggtg 180 aatcttttct aaaagaaaga aaaaacttct ttacaaacta tccaactcta ataacaacac 240 taattataaa ctagtctact acctttatta taacagcaat taaaagaaaa aatcgtattc 300 actgacaaaa attcgttctt ttgaatgctt atcgaatgtt ttaatttttt taaaaaaata 360 tataaatatt tgtaagggaa ggatcagaat taaaactctc tcccctcaat gaaattgaat 420 tatttgtttt tcttgttttt ctttttttaa aataaaccta tggatttagt tggtcggtcg 480 aattaaaatc gtgaggtcgc acacgcggtg tcttgtggat tcaaaattat gattatttcc 540 atcacccctt ggctttttcg ctccattcgg ccatgcctta caaatttcgc tccactccca 600 ttcttctctt cctctcctct ttcaactgca ttgaggccga tcctttaggt aaatggttct 660 ctcccatttc atctctatt cctctgtttc ttttattt acttgttctt ttccagccg 720 gatcctccat ttctgtggtg aaactgaatt gttcttatcg atttctgtt tgaattctgt 780 ttttctctgt tgtgtctgt gtgtgtttt aatttgttt ggcatgttga agtttaaaga 840 taccaaaagt tgcgctcac tactttccag ttcgatggt agctgctagt tgtaacgctt 900 acgttctttgg tttttgtt aaaattttt tgcttcttgt tgtttactgt ttagcaaaaa 960 gcatggggaa tactaccaaa gtcccgaact taatagatag atgatcatgt gctaagaagt 1020 gcgatacttt ccgtagctga tacgtgacac agtgtctgac atttgtttga cacatattag 1080 aaacttgtta gtataacata tgtgttaaac aggcatagaa cacctgttgt actaaaaaaa 1140 atatttgtat gataata atactttga agtgtaaaat atatccagct aagttttttc 1200 aagtatacaa gtgcattaac tcattccctc ttgattttct ttggtataa aaattatata 1260 tattttgaaa accgtatact ttataaatg tatccttgtg cattatgtcc tagatttta 1320 gatatggtg tgttgttgtg tctatatcgt gtcgtatcaa tatctcgtat tcgtatctgt 1380 gtttgttaga tcatatgtat aagcgaggac agctatttct gatgttacaa gaccttcttc 1440 aaattttaca ggaaatcatt caatttgaaa attcaagatt acaaatgcaa tctaaaccaa 1500 acttcaagaa caaaaagtgt tatttgttaa tatccttgcg acctcaccca aagtatctat 1560 tacaaacttc agaaaaaact tcataataag ggttgggttg aaaaaaaaca tgaagaagtc 1620 ccaccccaac ctaactctaa aaagcataaa aaattcaacc caacccaaac cttacaattt 1680 gggttgggta gtccgtgttg ttcgggttgt cgggttattt gaactcctag ttttagctaa 1740 gtgtaaactt atttaaggat gttgaaggtt agcattgatc tttctctctc aaatttggtc 1800 aagaggaatt attttttgag tcattcatat agttccattt tgcttttgag catttgaatt 1860 gtttgttaac tacttctttg attaatatat tcgaaagtga aatttccttg gtttacttta 1920 ttgatcagtg tcccatttta ccagttattt cagttctcct aataaccttc attggacttg 1980 agttcggtta acacaaaaca 2000 <210> 47 <211> 2000 <212> DNA <213> Muskmelon <400> 47 aatgtatcgt gggcatttat tatgacaata gtgtaaaat gttgttaaca tatctgtgta 60 ttgtcgatga tgtagatcta ctacgccaat agttagtt tccatcggta tatctatgaa 120 atgtcaacgc cttaaaatag ttgcatgggc atagacttgt tttttagaa aaatatgttt 180 tatatttgta tttttcac taacatcctt ttggttttgt atctaacac aactcaaat 240 atatcaaata ctgaaattca tttcctaaaa aagttacaat tgtttcaaat ataagtcacc 300 tgaatgaagt tcttaaaca caagaattg ttccttaca attackacata agcaaatag 360 taagatcgtc caaataca aacattacat aactttaga ccaacttcta atttgttttgc 420 caggaagtga tctccattga aagtttgtct taaaaaaaaaaaaga aaatagaa 480 acatattca taaactagta cattttgcac cttacatata tatacaaaa ctttacctac 540 tttaatttct tgaaaatcta aattttgaat tagaacttt tcttacaacg ccaaacaat 600 ataacttat aaatcttagt gatggataa taagttaata ttcattggtt gattgtatca 660 ttaccattt ctttctttg gtgtgagaaa cttatccaac taaaatatt cacaatagta 720 gggcgggttt gtcgtggctt tgtctgaatt tctacaacat gtgtaaatat tttcaactgt 780 tttatcctct aagacaattt tcctaaaaac aatcatgttg ttcacacgag atttccaagg 840 aaatttaatt caaggagttg aacttgtatt tatgttgatt tgatgcctat gcttaatttt 900 aagatttgag aaagcacgtt atatttgtaa agttggagta ttggaagaaa agtttgtatt 960 ttcaaaagaa cctcaatatt cgagatcgac ggttggcttc aaaagttagg aagtctttga 1020 ctcataggag aatcctatct agactttatg caatataaag agagttctgt tttatggact 1080 tagttggata aataattaat ttgattcaac ggtcataatg aaaacatgtg acatcattat 1140 attaaccaa tttatatctt tatacacat atcaactttt aatagttct aaattcaatt 1200 atttgtattt atcatcatta attaaataaa taatgtgaca atttgtgatt gatccaaaaa 1260 tttcatattc aatctatact atattagtta agcttaaaat tttactaaat gcttaaagtt 1320 ttggattatc gagcttccta ccaaacaaaa gcctctattg cacatttaaa atatagaata 1380 gtaggtttat ataatatgaa agattgactc ttaagaccat actctatgac ctaatgaaat 1440 cgacatttat gtaattgata attaataatt aataaaaaaa gtgtgacaaa aaagtggaca 1500 taataaaaga aaggaaattg tgaagcatta gcatccgaat ttcgaagaaa acaaagggcg 1560 ccctcagatc aaagaggaca tactataaag tctccacgct atttcaagaa ttggcgtgat 1620 tctcaagcga catttccgta attcaccaca aaaattaaaa acaaaaaaga actcacagat 1680 tctgatttga cttttgaaac cccaaccccc atcatctccc aatttaattt tccctcgata 1740 tttatccaaa ttcagaaaca taatcttgac aattttatgc tccattcttc caatctcagc 1800 cgtacgtttc attcaaactc caattctccc ccactgcgcc ttccactacc tttttccttt 1860 ctattaaagt gtcctcacaa actcacctcc tctctctgtt tctgtctgcg gtaggatcgc 1920 cgactccgga tttacatttc aggggtcgaa gatttgttct ggggtttctt taatttcttt 1980 atatatatac acacacaatc 2000 <210> 48 <211> 2000 <212> DNA <213> Muskmelon <400> 48 gccaaggaaa atgaattgtc taagaagaag aaagaaaaga aagaacattt tttgcaaggc 60 tacaaatcaaacttaaatt atccacgtga cacaact tcagagagga aagaaccctc 120 taaaactccc ataccttg gcaatacca tgacaatagc aaaataaa caagtccatg 180 actaaaata atattgttt tcattaaatc tcaatattc atatgtagtc cgctccgatt 240 atgccacagt catatatca gttcagtatt ttaacaatc aagtagacat actaaagct 300 actatggaa acataaaaa gatgaga aggaggtta aggaacctt tatccctgat 360 ggagttcag taaaactgag cttgtaggta ttagtacgaa agctgtgaaa tgaacaacct 420 tggccaggta attgaggcac cccagattt cctttatcaa cactcaaa gaagtaag 480 aaagttatca cttcaaacc siactcccaa aagcagctca tcattttcca gtaagttaat 540 actttgaaag atcaaatca aatctacaat caattagac ttcttaatag ttattgccac 600 gaaccatgca ttgtcacgt tattaagact atggtttgca ataatctcct atctggttgg 660 atcactactt atactaggca cagcataac taaagtagtt tcccagagaa ggaagaaaca 720 tgaacctggt tgggtccatc ttggcagtaa aggatttaag ggagaagagc aaaccaaaca 780 taagtttag gtcttgctgg ggattcaatg tccgagggg ccgattccac tccctgtaga 840 acagcaaac tccattccta ttgaaatat acatcatatg cacattgttt ccagtcgctg 900 tcggtaccgg aggcgaggga ctaatttctg accaccaaa gaactgcatg gtttctggaa 960 taaactaac taatcaat cattgtcat aaaatgat ctacgaatct aagattctaa 1020 storm atttcactca actctacaat cagtaccta gstormstorm taatattca 1080 atcattccta atattcatt gaggttaaaaaaatagc gattgtca caggtaaaat 1140 ctaacccgac ccaatcagg atcactaaa gcaagaagct gtagactcg atcaaaata 1200 acccagatgc atttcccttt ggcctctcta cagaaccact caatagtt agaaacaat 1260 ctagtgtaaa attgggagtc ctattcatac atattccaa ggaaaatgga ttttacttat 1320 gcatcgtata agagactgtg agcagggaa atggagaga taatcaccaa tgagctggat 1380 ggtgacagat tcagagaa gcatcaaat caaacaacgg agagcagaa gatacctcaa 1440 agagchagaga ctgcaagta aaggaagcga tcaattcaac gacgaagctc ttgattcgtc 1500 aggcaatgat tgccggcgac aacaacgtca gcagatcgga gccttacggc accggagacc 1560 cctccgacaa ggacagagtg aacgaacgtc gtttgtgaac ggtgtaagca aatcgatctc 1620 tcggagtcca actccaaagt actgtttcga tatgcattaa tacatttgat ttttgttata 1680 tcaaaaataa atattatatt aaaatttata aacattaaca aaaaaaaatt aatttcacat 1740 aatttaaagg accatttggt aatatataca aaattgcaaa aatcaaattg ggcctatttt 1800 gttgttattg gaggcccaag atgggtggtg ataaatatgg gcctccaaaa gaataagcaa 1860 aaaaccctaa tttcctctct tcctctcttt cccaatacta taaatcttca ccattttcct 1920 gattagggtt tttgttcgtt cttggccgtc cccttcatcg ttcccagaga gagggagaga 1980 gtaagttgca atagtaaaac 2000 <210> 49 <211> 2000 <212> DNA <213> Muskmelon <400> 49 aattagcaaa ttgtatgtaa caacgtcatg aagaggcatt tcatcgaaca atttaatagc 60 agagtcaaga tggcccagtt ttataagttt attgatttct cgattcttaa ggtaaatgag 120 atcgcgagca tggaaatgca gacccaaacg agaatatggg tgtggtaaat ggaccggtga 180 tgttgtggct acaaattcgg attttacagc agtaatagtt ctgacgaagg aagcgaattt 240 agtgaccttt cgcatcacag tttaagctta tcagagacct gaacaagggc tctagctcta 300 caacttagaa aggtttgata tggtccgtga tcgggaggga ccgaataaca ggcgcttaaa 360 ttgttgttca taaagaagag gattgtcgtt gatgttattt aaccaagaga tgattagtta 420 tccacatcca cagagagaac agaagacggc tttctaaatc tacaccgata aaaataaccc 480 taccactttg tttctttaga aaagggtcac attctttaaa aacattagcg tcgaggatta 540 atagggtata ttgactaatg ctctgtttgg atttcgagaa ataccaattt acaattgatt 600 tcaaattaat tatgttttgt tgttgcacga aagataaaaa gaatttaaa ttcaaaaagga 660 tctcaaatct tatttttaac ttaaaaactt ttatgaccca aacggtttat gtatgattta 720 aaagtagaat acctctgtga attcttaatt tttttttctt tccaattacc acataaatat 780 gaaattttaa atacatttat tttaaatttt atatccgaaa caaataata atttaaaact 840 atttctcata tatgaagtgt gattcgatct aaattataaa tttacatcta 900 gttttgatta ttttttttcg ttatagacta aattgttaag aaaataacat ttttaatcca 960 aagttttgaa gaatatatga cttttaaaat ggtatttatc tttttagtgt ctgattttta 1020 aaaaatggat ttcaaaagtt catcaaatag cattgtattt ttatttaaa taattttgac 1080 atttaaaatt agagtaatgg tttataaaag acacttgatc tctaaaacta tttctcttaga 1140 tataaatacg tatgattatt tttaaaaatc aatcaaaata ggtaaattgt aaaaaaaaaa 1200 aaaaatcaca tgaatagtag ttgtaattat gctctcaaac tttcggttat gaaaaataaa 1260 cattttaact tttagacgtg tcaaagttga gtcaagttgg accttcaaag ttatgtagtt 1320 atataaattg taatatgt ataagcttgt ggattcaatt ttatcattta tgggtccaat 1380 ctctacaatt atcgtaagtc tatgggtcaa ttgtaacaca tgtggagttt aagagctcaa 1440 ttttggacgt ggatgtgttt tgcaaccaac tccacacctt aaaaaggtgt ttttttttaa 1500 tttatcaaaa aacaagaatt tagaatcttt aagtttatct ttaaaaatca acggacattt 1560 tgaaaaccaa ttgaaactac tgttataaac ctaacaacta aaagtatatt ttttaagacc 1620 gaaagcataa atccataaaa aaaaaatcca gaactgaaaa tgtaactttt atagttgaaa 1680 atttagctaa attatacata ttaaaattca aggaccatat aaaattaaag tacctgatta 1740 aataataacg aattaatgtt tggtattttt aacctacatt agaaaaaaaa aacaaaagaa 1800 aaacggcata ctatttgtca agcgtccgat gggaagaaaa tccaacggtg agtgttagta 1860 ttgaaatacg cagttctcgt gaatgagcct ggcttagatt tgggaacaag agccaacccc 1920 tttcgaccga gaagccgtcg tcttcaccat attcgcctca accattcgat agccacgttt 1980 gaagaagaat taggattgcc 2000 <210> 50 <211> 2000 <212> DNA <213> Muskmelon <400> 50 agccaatggt tcaattaaca gctctagttc tagcatggct accgctggta acctttctat 60 gactagagac gtcttggagg tggagggtag ggcacgagga ttgaaaggtg agggtttggt 120 gaaaactcaa gcctttcaaa ttcaagaaag catgcttgac ctagtagcat ctggtgatct 180 tgggaattt gcaatggata ctcataccct tcgtctcttg gttctgctgg 240 tatatatttt ttctcttgt tattcttg tattttcttt tatcaatttc cattatgaag 300 atggaatctt atgttctatt ttcattg gatgtaggc ttcacaatg aaaaaatttgc 360 taatacgtttt ccagaagagg ttgctaaga cccgtaagtt cttattctt aacaatttcc 420 tcagtttaac aagttttatt tactaacata tccttagttg tataaatatg aatctattat 480 attack tcatttatct atctttac agggtgacca ttcacaca agatatact 540 tcattgaac gccctcctgt ctcacgcact tcggcatccc aggatgat gtctgtcctg 600 attcctgatc cggttgttag aggaagac tcagatggta ataatagt gatccattct 660 gttatcttct attattcattt tcattttgt attttgtata tattatata attattttaga 720 aagataaag atccatcctg aactttgtt tcaggtggaa gaccggaccc aactagtatc 780 ttggtgaacc aagaaaacat ggcagccatg aagaaagaga tgcgtttccg gcgctctct 840 tcttgtagtg acagcgacgt gtcagagact tctttattg atatgctgaa gagacagct 900 ccacaagaat cccatttgac aacggcggga gttccagagc catctgatgg aatgcaggga 960 gggaaaggtg ggaaaaagaa agggaagaag gggagacaga tagatcccgc actactcgga 1020 ttcaaagtca ccagcaaccg aattatgatg ggtgaaatcc aacgcttaga cgattgatcc 1080 attaggcaag atatagaaca gaaattgatt tttttttttt tttttccaat catttttgta 1140 gattgtgcag ttatttgttt tcgtgtttgt ttaaccctct tgtaagttgt tgtatattagg 1200 tttcttagag ttgtcagctg cgttgaaaca tgtggccggt atatgtattc caattcttttt 1260 cttttttccc gcagttgtaa atgatcaaat ttgagttggt caaattacca aacctttgta 1320 caggaacttc gaagagagtt gaaattttat tctttttcttt ttttgttctt ttatagagtt 1380 cgagattatt tgtatgaata taatcaaaag caaagcatgt aaaaataaaa tgatttgaaa 1440 gggaggtttt ctatcccatt caatgtgacg aatccaacac ttaaagtaaa tttgaaaact 1500 gtctaattta tatgtatgga atgtaatgct cttcaagaaa ttatcttatc ttctaatatt 1560 taatgggatt cacataaata tgaaatttca acgtttttct tttcctttttt gttgtgagat 1620 taaggatact agataataac cgacctcaac cttttaggcc aagaggtctg gagtctttat 1680 acttgaaaaa agtttacaca tattctaaaa gattaaaagg ttaattgttt ggtaaaacat 1740 taatgatgac gatacttaag gtttcattaa aaaaatattt ggaacaattt gtttataatt 1800 taataaaatt gtaactttga acattttgaa ttacattttg tttttccatt tttacggtcc 1860 tcgaactcat cgatactcac aatggagaaa aatatcacaa tgccgaaaat acccttcttg 1920 ttcccttctt atacaaaagc aacactattg gccttatcaa cggagcagca gctactctcc 1980 tttagcacaa atctccatcc 2000 <210> 51 <211> 2000 <212> DNA <213> Muskmelon<00022,45><400> 51 tggtgtaccc acttggtttt ttctcttttt ttcttttagc tttttgctcc taaatttctt 60 gccttagttt tcaaaagctt gtttttattt ttgaaattta accaagtgaa tagaaaaaaa 120 aaagagaaaa caaaagcttt taaaagcttg tttttatttt tgaaatttaa ctaagtgaat 180 aggaaaaaaa gaaaaaagct tataaaattt gacgaaattt gctgtatttt gtacatttta 240 It should be noted that there may be some inaccuracies in the translation of the biological sequence part as it's a very specific and technical text. The main purpose is to follow the translation rules as closely as possible while dealing with the given text. Also, the "213" should be "Muskmelon" in English for the common name of the plant related to this genetic sequence. And the "22" should be "400" in the original text form. The "21" should be "0002245" exactly as in the original.ctatttttct attttaaaa atgtgtctga acgaaaact tatattatga gatttaattt 300 tcaaaayatttaaccaa acagacttta gattgtca tcaatttga caatgattag 360 gtgcatttt taaagttatc ctaaagtttt ttttttc gtagtcttgc ccttgctttt 420 atcgttaaca aaaaattt tccttatata tatatacaca atttaactact caggtctgt 480 atttttcca cctgatttat ttaatatttt ttttttgc agaaaatcta tttgtatttt 540 agggaaaca aatgagtgaa gagatcatca agcaacggtt gcgatgttgc agcggaaaaa 600 tctttggttt gtcattctt gtgatgggggg tttaggtt agtatggtta tgttatttta 660 ggatgttgat ttttatttta atgagccaag agagagatgt ggattctaaa attgatgatt 720 gatattattg atgtgatata atatataat tttgtgcgaa attgctatt ttattttctg 780 tatgctcatt cagatcac ataatattt tatgtagctt tacttattga caaatatag 840 gttttaatct tgtgctcata siaacag ctatgggtga aattattttc tgattttatt 900 tggcaagat gatgtcagca ttgtgtaaat ttaatgtgaa tacactct gatttcttcc 960 caatgtgccc tctcaaatat tggcaccaag ccatttaatt gtaaatacgg aaaggtcata 1020 aatttccatg caagatttat ttcatgttta aaatgattgt gtgaaacaaa atgaaaaaca 1080 agaaattctt acctccaacc tcaaagtagt cgatatgtca aggttcaata tcaattttaa 1140 atatccatga atagctttga tatcttttat aaatgcttgt aatatata tactaatagc 1200 aatgtctata agttagtttt gagagtaata cttgttatag ataacaatgt tactctattt 1260 accactctac tattgaaagc ttctttttct tccatttatg aattaataac ggtcaagatc 1320 caattgcatg agttactttt aattaattac aatctaaaat gttaatataa gtctaaaatt 1380 gtccaatata tgtgattttt ttttctctc tcaaaccttc ccttcttttc attgaacttg 1440 tggttcaaat ttgatggagg acactgggaa acagcacaat tcaaagagcc aaagattgag 1500 taatttttg atttcagagt tttcatctct tcttcattct acacctttca cttctcatcc 1560 acaactatcc aatcaaccat tgccacgtgg catcaaaaat atccaaaact gaatgagatc 1620 caccacaaag ttcctctcat cactgtttgt catcaactca tcaagaactt catcatcaat 1680 cagaaatcca acatttcaac ttctcttagg aaatgacatt tttaccagtc tccaatgtca 1740 aaaactcaca caaaatccct ctttccaatc taaattttac aaagataaca ggggtaattg 1800 aagaaactta gcagtaagtt aacatattat agctttcatc aacccaagtt tttttggttc 1860 ctttctaaac tgtagtttgt tttcttgatc cattctaaat atttcctctg catgaaaaga 1920 agaaaggaaa agtgaaggcg aaacctgttt tatgcttcag aaaaccaatt cagagtaacc 1980 aaagatctga acttcagacc 2000 <210> 52 <211> 2000 <212> DNA <213> Muskmelon <400> 52 cgctcaaatt actaacatcc ttctctttct tgttcccatt cgactagaga gacactatct 60 tatccacctc agttggctgg gtgaaatcat tgaaactaac ggttgattgt ccagattgtt 120 aaactaccct atgttttatc atcttggtta catttatagg attgtcagaa taataattcc 180 ttttgaaatc atattctaat tggcacagga ctaaaataat gcctttctta agctgtaata 240 attagaatct aaacagtgaa gttagtaact gattgatgac atttccacga ttttcattta 300 tatcctgtgc agctattctg acatcacaa acatttctg atttcattt ttacttgtca 360 tccatcagtc aggacgat cggcgcgctt gttgacgacc tgtcctaaa tacaaagagg 420 cttatccgag ctacttcaag ggagattgac aagtggaaa gatgaatta ctcatttgtt 480 attacattgt acaagtgatc tattaggag aaccacaatc aaaactgaag aaaaaagaa 540 cgtgctggct gtctacgtgg cttttagagg tagaattt gtacaatgt ttagaagat 600 gtatttaatt gctctaaatc tcatgcat tggatttga gcaatcttaa atgccgaat 660 acttaatgta ttatcgtagg ggtccctaga tggcagatt atcatgtcca ttctccagaa 720 agaaagaaaa aaaccctttt tattatactt gttcatttta agctttct ggttgattat 780 aatgtcagta atttaaaaa aaaaaaaaat tactgtt tggcatcggt tatatgttat 840 atacaaccct agttaaaagg taaagttttg ttcattcggt cattagtcat tcctatacga 900 acgtcacatt gtgctttata atttcaatag gttaaaagta ttcaatag tttttaagt 960 tacctagtag aggtgatcat tggttgatcg gatcggtttt ttgacaaaa ccgccactga 1020 accgatcata gtcggtttag taaatgttca aatcgacctt gatacgatg agtaagatc 1080 ggtcggtcgg tttgtcgg atgggccggt ttacacttg gaaatactat tttgaattt 1140 ttcgaaatta atccctcttg tttcctacc gaccgatttt gggttttgtc ggtcagttcg 1200 atttttcgg cctatcttac tcacttat tacctaggga tgaatttca ttttcctt 1260 agttttagggg ttcttttttt atactttga atattttag tcgatgtcta gagtttaaaa 1320 atacacttg aaattataat atatttttt attattgtta gctataattt tacgtccaaa 1380 tatcaactca ctcgcaactt gtttaatcaa ccaatatat gtgtctggaa tagtagtat 1440 ataacttgtg gaaaatgact ttaaaagact ttttaaagt atttatta tgccaaaata 1500 tctatattta tgttattaca tcataca tatccaagt tacatattag atttgttaaa 1560 taattcaaaa tgagctaaag aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaggi gttccatata ccaaaaaaaaaaag 1620 ttgaagacta aaatagagat ttgaacaa ggtaagttag atttacaat tgcaatgg 1680 gagaccaac caccacataa caaaaatccc aatgtccaaa tggccaatt ttgtttagga 1740 tagctcacgt tatccaaatc actcaatcgg agagaccaac ttaaaggcca catctgccac 1800 gtcaccatac tccaccaatc acaacacagc attggatttc tcagcttatg agaccaatca 1860 caaacctgaa tccgacgtgg catgtccaca tccaccagta ccaaccatat agcttctacg 1920 ttctccacat ctaatcttca ccatttacac aatattcttc attcttcttt cctcccttca 1980 atccttcatc ctctccgccc 2000 <210> 53 <211> 2000 <212> DNA <213> Muskmelon <400> 53 aattctaaca actccggaac caaataattt agcatggatt gaaatataaa tcttcttgac 60 ttgcaaaaaa atcattgtaa tggtcttatg ttggttatag ttagggtatc gaaacgccat 120 acaggaatat gggattaaag ttaacttttg ttcatcaatt tcagcttatg aacttctaaa 180 atatcaattt tacctttgaa cttatatgtt attacccctt tcgattgtgg tatgttaatt 240 aatatctgaa tctcagtcct tatgaaactt ttttatactg tcacaaacat atgaagtttt 300[[ID=二十九]] attgtaagtt cttagaaatc atctaaaaag agtagtttgt tggactattt attttatttt 360 ttcttattaa gttgttttca cgccattca gtaaaataac tatagtgaat agagaatcaa 420 acttctaatc ttaagttaag gtagtaggt atatgctaat tcaataagat aatccgtgat 480 gcttgacatc tgacttatt gttataagtt ttaaattttt tattgtaata tttaaaatac 540 tagttttttgg tttctaata agaaatatt gacaattac aaatatttat acaaaatta 600 actagaatat atgatcattt tccttcgtgt tagaaaagg gaaatatatg tgtgtattta 660 tacatattag atattgtttt actatattcc attttcctca cgggaaatgg aggattgagt 720 gggagataaa cattgtcccc aagagaattg ggaatggaaa tgcaatgac atggccctcc 780 aaaattgt tcgcctaaaa atgggctttc tcactctca ctccgcaaga aaatatcgt 840 ttcccttcga attattcggg cggcaagatc tcaaaaccac atgttttct ttctttattt 900 ttcaagccta cattatttat aaaaataa cttaagcaga gattatgta attcaagtc 960 catttttcgc ttcacttagc aaatcatta acaaatctgt aattttgttc aaattagc 1020 tcaccaatta tgttttagcc cactaaggcc cattagacat ttttaga aaaacatgaa 1080 ccgttggatc aagatgtgtg ttttcttttc tttttctttt tatttttttt gggttttggt 1140 ggggttttgg tggatcatgg tggatcaatt cgtagcttta gcaacctatt attatatgga 1200 gggaaagggc gtattaatct gttagcgccg tccgggagtt tagctttctt ccccgagcct 1260 cggtcttatc ccctaactcc aaaaccctag cccaaaggta atccactcct tccccctccg 1320 ctcttcatct ttttctattc atcatcttta atctgttctc ccttttggtt cttagattct 1380 tcttttgttg gattctttta atctttactc atggttggcc ttgtaagttt agacgacgtt 1440 tttatacatt ggttaatcct gcttctctat ctattcgcac gctagggttt tcctattgtt 1500 ttctattctg ctctacttct gcaaggttgt gttcttcttc gttcaggtcc ctttttttaa 1560 ccgaaattaa attaatgcaa attcgtttgt gcttctaatt aggaagcctt ttggaacatc 1620 tcgacatttt gattgctgca tttcatttcg ggtatatttc tatgattgaa ggatgtgggt 1680 ctgttcactg catggtcatt acttatgcag ctatgcttat cgagtccatt atgtttgtgc 1740 aatctgtttc cggattcata attttttagt aattgatcag tagatgaaaa aagatattgt 1800 aatattcctt gagtgttgca ccagtcttgg tgggtatctg ctcctgctct ttgcttgtgg 1860 attttacttt tattatatct gtattattcg aaatgttctg ttcttgttat aacttatacc 1920 cgaagatgtg ttcctccccg cgtctagcgt tgtgggttac ttatgatgga catggttttg 1980 attctgtttg gtttgtgcag 2000 <210> 54 <211> 1547 <212> DNA <213> Muskmelon <220> <221> misc_feature <222> (1)..(1547) <223> n = g, a, t or c. <220> <221> uncertain <222> (1)..(1547) <223> uncertain at all n sites <400> 54 ataatgtgtt gatgttgatg atcatgcatg gtatattaat ctcatgatta aagacgttaa 60 gattaatatt cattccatgt ttatgatggg tgttcttagg gttgtaccca tatgggtgtc 120 cctcgggatc accacctttt ttatgactgt atggttctac gagaccacca gtctgtcatg 180 atatgtttat gaatggtacg acggggtcac ttacagccca attgcttaag tgttccttcg 240 ggttcactga agacctattt ttcctaggtt ttcctttgac ttcagcaaaa atcagttttg 300 tcctaggtgt tcctcgagtt cactgaagac tagttttgtc ctaagtgttc ctttaggttt 360 atcgaagatc agatgtgttc ctacagaatc attagattgc aagtgttcgg gaacacatcg 420 gtttaggggt acttctttac atgaacccta atggaaattt aacagacatc tagcggaatt 480 agtagttggt cccttactga gtatatattt attackcactc ttttgtt taatatttca 540 ggcaaggtt aaggtagagg aaagttgacg agtgatagaa aaggatctgt gatagtcat 600 atggggactc agtttcgttt ctgcttctat gtatcagtgt ttcagtattt tgtttnntaa 660 tgaaaattta gtctctcctct attcaagaa gtgtctcttg ttattgttta tttttagtaa 720 tgatttcaac ttagtataaa tagttggatc attacaaata atatattggt gatatacttt 780 gtaatgatac attgagttat attattcata tgtttatat acaaactgc atattaaaa 840 aatgaaaatc acgtaataag tatatcaca aaatataca tattaca gcacgtcaca 900 aactaat aaaaacta tataagta gataagca aaaaaaaaaaaaaaaaaaaaa 960 acaaaatcat ttgaaattaa atttaactca aaatacacat cgaagaaagt ggagaaaaat 1020 cacaatagag ttaaattact ttgattaata accattatat ttcatattga aaataatatg 1080 tcattagtat tttaaaatca agattaagat aggaagaatg aattgctctt ttcgtataaa 1140 aagggatgat tggggcctta cgaaaggaga aaaatacata tgttatcgaa aaaacaaatt 1200 atttttcttg taagagagaa tgattatatc cttaaaaaaa tgaaagaaag aaacaatcat 1260 ggcattaaaa aggaaaataa ataaattatt aaagggcagt tcgataataa taacaaattc 1320 aacgagagta ttaaaagaaa atgagaattt gcaaaattta aacaaatgtg tatattaagt 1380 acagccaatg caattttcaa attttaattt atttggttta cccaaaattc aatttctaaa 1440 ttgagaggag gatatagtaa attcacacgc attatcccct tcgagtttca tcatctcacc 1500 cattcttgca tacagtgcag ttacaattcc ttcattctgg atagaca 1547 <210> 55 <211> 2000 <212> DNA <213> Muskmelon <400> 55 aaacacttat catgttatgt atcccacatc gaaaagataa aagagacttc atgatcttta 60 catgatatat gagttactcc ttcgactacc attggttttg gagatggatt caacccaata 180. atatgaatct gacccaacaa tggtcaactc aaagagacac catcttgaga tacatgttgt gtacccatat tagatgaatg actatacctc gcaatattta taacatacat gaattacttc tcttactgta atttggtttt gacgtggagc ccatgattat ctaattaacc ataactggta tatgatatat tagtagtaa cccgaagagg ttctaagata aacacagaat tcaatagaat cagagccttt ccaatgatat ggctttagat gggaatgatt tgaagtata tcattctacc acacccttta tatttgtctg tcaccagaaa tctcatcttt tcttgaggta ttattcactc gaaaagaggg aggcattttt gggttaccca tctaatgcac gatgaactaa gggaggtcaa gttctggga tacagctagg caaccttcac agtggataca ttcgaacaaa tgataaatgt gaaaatgaat catttcatga gtgtgactaa cccaatcatt cctccttcta tatctttgaa tcccacagtg agtcagagta aaagttccag caacaagtcc tacaacccaa attctttagc tatttcttcc accagaacaa aaccaagca aaaatcagcc acaaacacag ctcaacaatc tataaaggcc aaaatacta agamagtcacc attaccacat tgaagccgt attttccac 840 agactttgcc tgcaaatag atcacaaga cacgatttca cattggacag acgccacagc 900 tccacaatct caatttcaat caataaag tcaatcaag ctaaatagca agtgtatggt 960 accacgaaag cagcatggct gacgccactg aggctgtaa gagagaaac aaataagtg 1020 Tagagata agtgaatag aaaatcaat cgatagata gattttcaga ttaccatttt 1080 tacgggaatt gtacggaccc aaacaciaac cccatagagc gccggcctga agatgaacag 1140 gggcaggaaa ttcagaaa gaattaag aaatgaatc atagtttgag aattattcg 1200 taaagtttac cgttccgacg cgaatgctgg attcgacggc gagggaa caggaacga 1260 cgccgttgag ttcgtcttcc atctccaat tctcaatttc cttcggaggt ccgtatgctg 1320 agagctctgt gtctaccaag ttccaccat actacgtcgt ttggattt tattttatt 1380 ttctttcctc tcttttgcca aaaaagaaa aaatagtatt acctcaaa acctcaaat 1440 aacatatttg ttgtacaaat tataattagt aaacatttgt cattgtgagc ttggtatgta 1500 atattaacac gaactttatc gctaataatt tagacgttaa tgaataattt gagcattgcc 1560 ttcttatatt gttattgtgt ttataatagg attgcttaca atgtaaccta gtatgttgtt 1620 gagctcgtta acttttttgt ttttcttgaa tattcaaagt taaaaaattg tacaagtttt 1680 tggtgacgtt ttcttactac attatcggga tgaagatcaa atatagctta gattagagaa 1740 gataatcatg ttgatttatc gttaaacttt gactacaaaa tccgtttaat ttttttttgg 1800 atgaattagt tatacaattt aaacttaaaa ggggtgaatg aagaaagagg atagttttac 1860 aaattcgaag tgaaatgagt tatttctgct taaagaaaac aaatctcctt cgtgctttaa 1920 aacacaaact caaaacccta aattcagcgc cgattcttca atacatctct gcaggaagtt 1980 agggcaaagc agaagcaaaa 2000 <210> 56 <211> 2000 <212> DNA <213> Muskmelon <400> 56 acttccaaaa atcagcctca tgggatattt aaagaaaacg taaattaaaa ttagcatcat 60 ttcatattga acaaactaca aaaattaact ctaaaagatg atggtaacta caactaaacc 120 ttcaattttt cattgtaaaa atcgaactct taaacttgtt caaatattaa aatttgaccc 180 tcaaacttaa aagagctaaa aaaagacctt caaatagtaa aagtagaact ctcaagctta 240 tagaattatt acggttatga ttatagccat agatgattca atcgattttc ctccaagatg 300 atggagtata attcttcaaa tctagctgct tagatgttat cacgataatg aaatcatatg 360 ggaactcaac aaaaagaaag cacttaatgt tgaaagacat tattctttgg gtgttgagtt 420 gggcgaactt gatttttatt attaatccgc aaaggacctt ttgagtaagt tgtggcaatc 480 tttattggag tgctaagatt tgttattcga aatttcttgt tttgatattt ttccaactaa 540 aactaatttt tttaagaat gcaccttcaa ctgatttcat gcgtgtcctt ttgcaagact 600 cgcatgggac ataacacatc atcttatatg gcaaggccta tgtgtcagtg gagatttgac 660 gtcaatttct ttccactgag agtcgtcctc tttgtgatgg cagaactttg gagagtcatc 720 aaaattggtt ctttgaaaat gtttcttatt ttgatttttt tttttgaaag aaatgagagg 780 aataagatat ttttacgagg actctactag tgggtcaatt tgcccgcata tggatatgca 840 taagagtcct ttggagaga aagggtatga tggaagaca ttgcaaggc ccgtccacta 900 actttctatt atacattag gtggaagcca cccatagcaa tgtcttggtt gaacactgat 960 attacktgaa accatgcatt tagatgtga aatctcgact agatgcttta ggaatttgga 1020 ttgtgtctgt ttgttgaat tcaagttcat tcctaaatac catgaagtta agatccttga 1080 agcaatgaag accattatt tagatcctta attcaaatct ctttactaaa gatgattgtt 1140 tataaatgat caatttgttg aatgatgttc tacttgatat ctctaaagca tctcttttcg 1200 gtgagaagcc cacaacttga atagtattcc ataaatcatc tattttagt ttctatcatg 1260 ttctttaaca tcaaacatt tagcgcact ctcttataac tagacttag aaaaacacga 1320 atcttccttt cttacgatat atatcctaaa tggttttcta tatttgtgcc ttacaata 1380 atcaatctt tttctatttg atattgtcat aaaataatac tgataacata gtttttatgt 1440 tttattaca cctacaaga atatggag acgttaat atcttcaatg tcgatattga 1500 atcattttat ttatgaat atccacgcgt caaaaatat tttaatcatt aacttctagg 1560 actaaattca aacattcttg gaaccataga caaaagaaca aaatttgcaa cctcaacaaa 1620 caaaatttta tctttacatt tgcggctaca attcacaaat tcccaaacca tgatagaaag 1680 gccccaatct cccacgtgat aaacacacat atggcacgtg accaaatcaa aatcatccac 1740 atgatgaaaa cttaatggac agctcggatc ccaacaccca ataaaaagca gccatgaagc 1800 tgacgtggca gatttccccg aaaacctttt aaataataaa caataaaaaa atatatacat 1860 aaccgttggc aacgtttttc cctccacaca ttttcccatt gccttatctt tctttccctc 1920 caaacagcga gggaagaaga atccaatcat cttcttccaa taatttctaa aacgaaattc 1980 tgctcgattt tccctctcca 2000 <210> 57 <211> 2000 <212> DNA <213> Muskmelon <400> 57 tgggtaacat tatgagtttt attataattt aaatgaagat caaactttaa gttgtagggt 60 caccatagga ataaaatata atcaataagt tagggcccct tagtcccacc tcgtaaagga 120 gttctgtcat acatatacat tatgatatta attctatctc catgagtcat acatgtgatt 180 ttagtacttg taatttcat tctttttcct attataatttc attcaagtac tgtcaatg 240 gttaggtatt gaatttatt atagactcag attackcatctt cattaatgga atggaatgt 300 tatattctac ctctcatttt tacacgttga tgataatta gagaaaaa aattattatt 360 tatattgttt taattgtgag atattagttc aaaatgtaat tataaaatg atacgtgtct 420 tataaaa ttaacaagt ataatttaat aaaaacac atacacactc tttaactaaa 480 agacacaact cacctaatgc tcgacttaa atcactttgt gtcgtactt aaccatcaa 540 gcatgttagg gtaacaca taagatt tttgagtta tgcatgtcat ataatgtcac 600 ttccaatttg acttatcttg cttgcttgat tcatgtatat aaaaaaac atgaaaagta 660 gtgtaaggat accaattacc tactgatttt ttttaaaagt agttgtcta agacgtgtta 720 attactaac ttagtcacat tgagtttta gttctaactt attackacata agtaggtat 780 ctcccttact catgtgtgtt tcgataatgt caattccaa tgtttgatta accaattgg 840 gtaatttaac attaatattc attatataat atttttg gataccgac atctaaaag 900 aaatcaaat gatattatt aggaggtgag ttttaagg agaggaaat aaaaatat 960 ggcatcaca agaaata tatagaat agaatccga CAaggaa agtggatgcg 1020 tgttagtact attgacattg gcatatgaac ggttggttg ggcctcaaat aatttgcatt 1080 tctaactttcc aacacctaa ttcctttttt tttatccata cttgcaaata tatattata 1140 tatattcac aagtagtttta atttatttga tataccactta taagttttaa attgatggta 1200 gtgtataaat aaataattta ggattaagca tgtctatgaa cctttgaaa tttgatggag 1260 tatatataaa acagaatact catgggttca tttaaaaat ctaatagtaa atgtattttt 1320 tattcattt aacattttc aaacttttaaaatttaaat tattcttaaaacacgtgtg 1380 gtttcgaacc atatggttaa aaatattgag gttctctattt ttgcaaaaa tttggaaacc 1440 ttcatggaag ttgattaaa ttgttgtaat tagttat ttttcttt ttgtggctt 1500 aatcatgcta tgatgatca ttcatc atttctataa tgtaaaaca tatattgat 1560 gtgtattgta aatttttg cagagtaga aattaata aaaaaaga gagaaaata 1620 attataaagt aatataaagc tattaacatt ttaagaaaaa taatagtgaa aatgaaagtt 1680 tcggacaata attcaataaa gaaatttgta gatttcgatt aaaatttcca aaattaagat 1740 tttcattaac acgtgtgcct cgcaaccgtc tcctacgtta tcccgtaagt agcccaatct 1800 atcccattct tacacaagcc gtcggcccaa attgattgta ggccatcggc ccactcaaca 1860 cccacaaacc ctagcccctt gctcctcctc ctcctctttt cacggctgct cactccctct 1920 ctttttacac cttctccttc tccttctccg tccctcttcc cttttctgct actatcttca 1980 gcacttgctg agcttcaacc 2000 <210> 58 <211> 1591 <212> DNA <213> melon <220> <221> misc_feature <222> (1)..(1591) <223> n = g, a, t, or c. <220> <221> Uncertain <222> (1)..(1591) <223> Uncertain at all n sites <400> 58 aatgttgatt tacccttgct ttgtttgaat ttcgtcctcg tggacttgac ctttggtctg 60 cttcgtatag gactatttac ggctgccctc atagtccaag tccttgtccg cttacccta 120 tactctctat ctctagacaa tgtgaagcgg gcccttctat aatattgggc cttgaagtttt 180 tgggctttgg atctgccgaa ttgtgttggg tttctctccc aattatttc atttctttat 240 tcaaatatt ataaatatgg aattttattt tatttaaaat ataaaagtta aaattgaacg 300 aatccaaaaa taatggaatc aaatcgacgt tttaacatat ttttcaatta tgtttttaca 360 ttcatttcg tcctacaaaa atattcccac ctttatttcc tcgatatcgg aggtcacttt 420 gtatgtttca ttcgggtgat gtgatataga tcgagtttct atgcttgatt gactatggaa 480 atatatttta agaagatgtt ataaaagtaa aataaatgtt ttgattgtgg atataattat 540 attttaaaca agatgaggaa taattagatc cgaaccaata atcttgagtc aagagtgtac 600 attgaaagtc gtatattaaa taatggttga gtttataata atattgatag attgcagtta 660 accatatttt ctcaagttgt tgaccaaagt acttatttta taaacagttt agggaatgtt 720 tatgaagttt tgccaagtgt tttgaaccta tatgagtatt gacttaattg gtatataagt 780 gcattaacaa tcaagaggta tttaatttga atcgtcctac ccctatcatg ccaaaaaaac 840 aattatatgt ttgtcatatt ttattgaaag tgttttcagc gcaatttagt ttgatttgcg 900 tacaaaacat gtctacacgt atcgagttag tagtaatggt tgctagttaa gactgtgaac 960 taaaacttta aatttacatt aaaaanaaaa acattatggt cgtttggtcc tcatatgtga 1020 ttgatagata ttgattaatg agtatttgtg gttgttgcca acaataaaga tgtagacaag 1080 tgaactatgt tggttgtcaa atcttgtttg tatttgttat gtgtggttt caccaccaat 1140 gttgtagagt gtcagatcca gaatagcttg atcatttttc atatatatct agagactcaa 1200 ttagtagata aaaactataa gactttgact tatttctctt aaaatgtctc ctcgttctgt 1260 acaatcctca acaacgtttg gtgactttaa aacatcacaa gaatctaaga agaatgatga 1320 attagatgca atgcaaagat ttggacctta attttgttac tttaaacttt atatccgaac 1380 attggagaag gcaagcaaaa agcgcgcttt agaatcgcgg tttctttggg ccgagtgggt 1440 tgctcataac agcggagggtt tgcttttctg ccaagaaaac ccctcaaaga aaaagggctt 1500 aataagcagc tgctccattt ctaagtgggt ttagccttta gcacggaagc gccaattcga 1560 ttcaactctg atacactgca aaaattccgc c 1591 <210> 59 <211> 2000 <212> DNA <213> Muskmelon <400> 59 aaagaatgga gcaagctgat attgctaagc aaaagcttct gcatgattgt gaagttcttc 60 accaccgcct tcaagattct actgtcgact ttttcattga gcaggaaaat aaactaattt 120 tggaaactgc ttcgacagct gacgacgcaa tagatctgtt ggcaacatct gataatcaca 180 ttaaccttct tttagcagag gtacttgcgt ttgtttccat tgaaatgcat ttgcatattg 240 aacttcttca tcctgaatgg cacaagtttt tgtccataca aacaggcaaa gcttctggct 300 catgatgcta acaacagcga tgacactgct ggatcagccc gtccaaatgg aactgataaa 360 ggggcagctg accaagtatt aagtaacata ctagcaaata tgcttgtcga aaatgcccga 420 ttaaggatgc agatgaacgc cgtcatccgc tgtgttctaa atgcaaatgg gacaagtgag 480 aaagatgaag atgaatctct caaggaagaa ctgttctaag caagttttta gaggaagaga 540 ttcctgaatg cacatataca atgaccttat actgtcgtgg caaaatgg gagagctgta 600 gattttgaat aaatgcaaca gatgttgccc attaatttgc aagtcctgac aaatttggtt 660 gtcggaggtg tagaatgat gtatcaatta atattaac aaagtgcctt ttggcttggc 720 taatcatggg cattgaga ctttgcactt ggtaagagct caacaaat ctgggtggct 780 aaatttagtg ttgatttaat ggaatttcac tgatattcat gatctgtctc ttctccttc 840 attgatatat tatctctca gtaaactcct gggcctgatg cagaattgct tttaccatc 900 tgcatacaga gagaagtaa aaactagctc acgtggataa agggaattt ctactgacat 960 gttggcatta gaagaaaatt ttgaaagagt tctattacca taacatc tactccgtg 1020 tattattgaa actattattt ctcttaccccg gagatatta attaataat ttctattac 1080 attttgaaga tgctcgtgat tattgataa atgatgaat cattattttg attacgttac 1140 aaaaaagtca aagagagta aaagctatc aaaaaat tagtatata tacaaaaaaa 1200 gtgtaaattt atattaca ctgagaata tacacttaag ctaatggggtt aaatatta 1260 tccattgaat taaatatggt ttttctgtat ttgtgatatt ccaataaata tgaagctgtt 1320 atactgtcaa attcatattc tgcctataca atcaatttca agtcactcaa ttttgcaaaa 1380 ccatatcata ttgagttcaa ataaaatttc atatctatat acataacgaa aatgttatgt 1440 ttttgctttt aatgttttgg gtatctttct aagctacaag aaaatgtaaa aatgataata 1500 agaaatagat tatattaaaa ttattttaca aatcaaattg cggggatagc tcagttggga 1560 gagcgtcaga ctgaagatct gaaggtcgcg tgttcgatcc acgctcaccg caaatttttt 1620 tcttcttttt tttcccttgt gtatcatttt aaatgggctg ttcttacttt gaactgcgga 1680 agcccatgaa agctaggccc aatttagaaa ccgaccatct caagggtcgg ttcgtcattt 1740 atcaagatcc gataacccga ttcgctccat tttagtctct gctctttcat ctccctcacc 1800 cattctcgct tccactgagc gggcaaggga gcttaacccc tcaaagccct agaaaccgcc 1860 attggagaag ctccactagc ttcttcttct atcagcgaac gtattttcgt cttgtataga 1920 cctttcatct ctggaaccga tcggaagttt ggagtttctt ggtctcagtt tgtagattag 1980 ttttatcttg gcgtctcaat 2000 <210> 60 <211> 2000 <212> DNA <213> Muskmelon <400> 60 gtgcatttaa aataatctag ttgcatgttc taggttcgat ttattatttg gggatttagt 60 tgtgtctgta tgattgaaaa aattaatgtt gatcttgtaa cacaattgtt tttccctcga 120 tgatttgaga ttatttcaac aatttagatc caatgtttaa aaagccacct tggcatcttg 180 ccttcctcat tcgcaacctg cctccagttg aagcctcgag gctcaaagcc cagtgcccta 240 ggacttcttt attaatttta cttaaaaata aagtttgtat ccctaaatgc ataaaatacc 300 cttgtgttta aggctttctg tttcttcgcg tttcacgtca ggtcagacca tgctcagcta 360 tttttccacc attcttcttc ttctctccca aagtctatca agtattttat ttccacacat 420 atattcacct acgccaattt ctttttaaaa ttttatagat atatacagtg cacctcacga 480 aaacaaagtt tgcacttctt cagttttttg tttcgcctca cacttaagct acaaaaggtt 540 attacgtttt agtaacccac tactcagctt taaaaacact atttgtatca tatgacgtcg 600 cccttatgga ataatttcac ttgattatcg ggttgtttca taaacaatct tactctgttg tacctttgac aggcctggag agcatgcaac tcctctcttg cttgagtttg agtacaata aaatcggaaa ttttactgca ttggagcctc tgagactgat aaaattctta aaagttttgg atatatcgta caacgagata ggttcgcatt cgatcgacac aaccagatat ctcttctcat ctccactgtc gcattccgaa gaaattgatt tgagcagtga tgaatggca acaaattt ctgatatggc aagttactgg gaagcatatt ttctattcaa agatatagc ttgatgcaat tggatataga aggaaacaca atatctagtg aaagtttcaa agcatttctg gtaaagattc ttcccaaact ccactggctt gatgggaaac gggtacaata gatatggctt aatttatcta catccaatcc tctgtccatt gtggttgttc atcccctga tgtaaaaagg tacgctacga actagcattg atcctaaatt gaagacattg gttttgattg cttcccaatg caaggttaag aactaaggat ttgatattgc atccaataag cataggttat ttaagatttt ggtgatagtg aaaattaggt gacatgtctc gaaagcttaa agggatacat gaggtatgga gatggagatg gatgtggtta icacatggaa atgaatacgg tgcccagtttt tttggactgc tctaaatcaa 1380 attttatcat atacattatg atactgtgtg ccaattgtat ttaaaaaggt actgaacttt 1440 acatttttgt tgtcccaaat tttgaaggat tgtagtttta atattctta taatactat 1500 caatgttaat taaaaacttc agtatattta caatttttct aaaaatgttt gctatacgtt 1560 tagttattat cttgatcaat tgcccaaga gaaaaattac cctggactat ttcccaaaaa 1620 catcttctag tcgtccatca gctatagtttt caatctgtg tggcccagt cggcccagtt 1680 cattgggcct gagaatagag atcatgacc ggacggccca aacctttc aggccccagc 1740 caagcctggc ctacaactt ctaacctaaa accttatccg ttgaagcaat ccataaaac 1800 aaagccacgt aagcaccag gatctaaaaa tgtatccaaa tccaccaatc tgaggccaca 1860 aatttagcct ctgtggctga atggatgtcg aattacaaga atctcgat ttctcctct 1920 taaatccatt taccctcaaaaataac aaaaagga agaaaaaaacatt 1980 gtcgtatta gcaxagaa 2000 <210> 61 <211> 2000 <212> DNA <213> Muskmelon <400> 61 acctagaact tctaaacgat aatctcggaa aaaaaattgg aacaaatcat aataatgaca 60 attaagaagc aagaaccgtt gacaaaagca agatttagag ggagtaaatt tgcatggttt 120 ggtgatgatt atttagttga atttagccta ctcttaggaa gtatccaata atcatacgca 180 aatttcacgt agcatatgaa gcaagtgcat cataataccg cataacctgc ggggttttgt 240 catctcgatt aaacacaatg tgaacatgat gatgtctatg tgtttccagc ttttgttcta 300 atgatgatag acgatagtgt ggtatagttc atatccttga tttaattgtt tccatgtata 360 ctatcgaatt tttaatatat aattaatgta tgaaatcaaa tatcaaataa tgattgtgat 420 ttaatggaat aagatcatgt ctaaaattgg taatagtaat aacgaagaag gaagagaata 480 ataaactacg atttcttgtg aatctcctag ataaattagg ataaaaacta cgagtaagaa 540 tagaataatt atactatata aaataggagt tacaaatttt gtttcttaaa ataccaagct 600 ctgttacaag aaaaaacttt aggtattata tcttcaacat tttgttaatt tgttagagat 660 tttaggatag tttgtcaact atgggtcttc taagaaactt ggtcatcaag caaatctaat 720 gactcgaatt gtccttgatc gatgtgaaag atccaatgac ttcgaattat ctttatgcaa 780 tgtgtaagat ctaattgtca taaattgatc tcatgtgcaa agtgtaagat ccaatgatcc 840 aaaattgtct ccaacaactt cttgaacaat aagataactc tttgaagaat cttgaatatt 900 aattttgaca tagagatt gatcttgaat attaggaaat aaggaaaattt tcttatgtac 960 atgcctgaac tccttcaaca tagcattttg aatcatatct cttctctagt aacttgtata 1020 gttgcaatat attttgcttc tgttgttgat atatcaacac tgattgaagt tttgaaaccc 1080 aacatatagc tctagtggca agagttaaga catatatgtg gtgaatttac ttctatcaag 1140 atcacaatct aagcagatat actttgaaaa taaagttaga ttatccatta tacaatgtaa 1200 tatttacgga ccatttaact cgcttagaaa ttagagttat tttgcaaact ttattgacaa 1260 atatcttcaa aaatttcata caccgtatag acactatcat aagatgttaa agaaaaaaaa 1320 aaggtgaatt ttccatacaa ttaaaaaaaa tcttaacta taaaggtggt ttcgatacct 1380 ttaaactttg aaaagtttca ttttaattct cgcacttatt gttttaaaac aaacttagta 1440 aaattttcgt ataaatttag aaagaaattt tatatttaca ggtggggaaa attctaaaca 1500 catagatgaa gataaataaa aacacgatca actataaact atacctatta ttaccttcat 1560 ccttaacacc atgcactcaa atattcatta attctctata tttttttcta tcttagcctc 1620 aaaatttact ttcatcctaa acttcgagcc ctcaaatttg cgttatttca ttcacgatat 1680 tcctttttta cgttctttca tttatggtat tcttctttac gttcttctat ttacgatatt 1740 cttcttgctt ttatagtgtt ttagatttgt tcataaacaa cgtataaatt gaaaacttta 1800 taaatttagg gcattaaggt ataattgaaa ttaaaaccat atttatagtc attaaccaca 1860 gtattattta tcctttattt attaaaaaaa aaatctactt ttagttttaa atttaggcat 1920 tttacgcaaa gctaattacg acataaaaca ccaaaaggag accccgttcg atcttcacat 1980 cttctcggcc agaaacgacc 2000 <210> 62 <211> 2000 <212> DNA <213> Muskmelon <400> 62 gcaatggcgg aaataacgta gcagagggca caaacaacga agaggactt cgttcttcgg 60 tgcgccatct gggaaagtga gaaatggtgg acgaaacaca aacgggggaa tcggattgga 120 tctctcagaa aagaaatggt tggattcgat cacagatcaa tgaagcacat tactcggttt 180 ttcaagaaga ttacaagaac ttgcttcttg aaacctctct tcttctgttt gaaatttttg 240 ggcgtagaat tgaggaccgg agcagtcgcc tgaatttggt cgtcgaatcg attccacgga 300 aacaaaaata tgaagaaacc aaatgaatga cttagcccac tcactacgct tggcgacgtg 360 ttcttcttac gaacggacca atcaaatgcg agcctgatga atatgggcca atcatattat 420 gccacgtaag actttacttt tgcccctgac ctatgggaag aaaattgtgg tcttttctta 480 tgtcaataga agaaaaataa aattatatga aagtcttaaa aggaaaaaaa caaaccatgt 540 taatattact gtttaaaacc ataacacaaa atcaattat gtttatgttt tgagactccc 600 ttatggtgtt tgctagatag tgtggatttt gtttttgaaa attgtttttg aattttgtta 660 ttcttaagtt tttttatccg aaaatttcat tctagaaaac aaaattatat aaaaccattt 720 taaaactaa tatatcgtgt tatagttttt taatgtaacg ggattacacg gcctattatc 780 Aattatata Tagatagat Taaaaaaaaatgattt Attaggcttt ttaaaata 840 aaatttaatc tctaccgctt ataactataa ttaagtcatt ttggtttaat aaatcatat 900 tatatagtct cactcgtag tattattac aaagatgtc gactttttat caattatag 960 actaaactat aattctctc gaggctaaaa ttataattta accaattta taaatgtaaa 1020 atgtatttat aataaacga atatagctt gtcgtcaact atattttagt ggataagtaa 1080 gattagtttt atgatttata atatatagt aaaacaca tttaaacatg tttgttcat 1140 tgcgttttggt tgatatttaa acctagtaac gaaaagtat taggtattac atttaattag 1200 catccaccta caatgttaaa tttttaagtc agttaataat ttaagact ctcttcaaca 1260 ttgacttcat gcaactaaa atggtagaaa tttcacacc attgtttatc gatacta 1320 cgtaggagaa tggcaaact ttcttatg tatgtgct tttagatgtg tctttacatc 1380 ccttatcaa acgaaaacct aattctaacc aaatcaacc aacccggtt gttgggttat 1440 tcttacaagc catttgttgg attaaaaaac caaaatagag gatgttcggt tcaagcattt 1500 taaagttttg ggctatttag ttcgaccact ggtttgttca aagtcgggtc ggaccaaacc 1560 gtgagcgatg taaacaacaa aggtctaaat tgggccggga tcagatgggc tgaagatcca 1620 cgattctggt ttccaaccca aggcccaatg aattacaaca aaaaagcgta ctcaggaaat 1680 ccgaatctgg atctcaacgt actctaacct ctcacagttc gccacgtcaa gaaaacacgt 1740 caatacttta ggcgaaaatc aagtgaagaa ttccccacaa taaggaatcg tatatccacg 1800 aaactatcca atcagcttac gccatcggaa gattcggaac aaagcaacag ttcaatggta 1860 tatcataggg tgagaataag tcggttccgc agactagtat ttcttagtca aactttacct 1920 gcttcaatcg gccgccgatt tcccgatatt tacaacattt agttccgatt tttccctcga 1980 agctctgaag tatcgtaaaa 2000 <210> 63 <211> 2000 <212> DNA <213> Muskmelon <400> 63 gatcaacctt gaaattttcc cacatactgt gttgtaaagt tgtccccaat ttcattcaca 60 aattacctac ttgaggaatc ggcagtaaga agagatcata atgtattttt gctactacac 120 tcgcaagtct aatcagagga tttgattaca atactcttgct gctgtaatag attcgttcat 180 aaattaatcc agattgaaaa gtcaagcttt acttcattttt catcgacaat gtagaaattt 240 tgttataac tttgtact tgaatctatt gctcctcgat ttgccccctt ggtacgatat 300 caagccatta ttttccaac tcttactcgc aacttcaacg catgaacttg accagcttca 360 acctataatc ttatgcatgt ttttaatgat taaagctgaa atagattgtg aaacgtacct 420 tattctcact accgctgcca aagccaacca agcttccacg ggtacccata aggtccacaa 480 tcatggcact cttggatgac atgtattggt tcctactgtc tcttccgggt tctcttatta 540 atggccccga aagcaacctc tcccggcatt ctcgaaattc ggctcactaa tattctttag 600 ctactaaaac acatgtcctc aaatttctca tttaaatgtg atctgagaaa gtcattcgac 660 ccattttagt ttaaataagc atcaagtcaa aaaccattta acgtgggctt aaaaatttac 720 agcagcgcag cgtacactaa agtttatgaa cgatgaaagt gggtggcaga agaaagcaag 780 aagtccgaga gacatgccaa aaagagtaaa agtcatttgt tggggccttg acagcaaggt 840 tccatatgca tcggtccatt gcagcatggc ggctcaaaat taaattttca cccttgcttt 900 tgcttctcta acctaccctt ctacgcatcg tgtctatctt ccttcacact cattttgtgg 960 taagctttaa cgcaacattt tcttaatgta atttaagctt ggcccaccaa tccctttgaa 1020 aagtttcctc tagatggtgc gtgtcaattt caaattaaca atttgaactt atagttctaa 1080 cccccatatt gtctgccctt tttctcttct tcttcttctt cttctagttt tgttctggtt 1140 taatcttttt cggttttctc tgtgcagggt agtagctttt aagcttagtg attttctctt 1200 gttaacaact ctaagcagtg aattgttaga gacctattat ttcatataaa tactagatga 1260 cttcgactca ttgattaggc tggaagctgt caaaattaaa gagtttgaca aatacccact 1320 aatttggtaa ccaagagcca gcaggaacat ttgtatttat tgagacaagt gaaagtttgt 1380 tattttcttt actcaaaatc tctctttaat tttatagata tagacattac ttggataaga 1440 aagggagttc accggccgga ggttttcctt caaatttaac agtgactgag gtctctttca 1500 gctttgtttt tttggtgtta ttactgtttg ctcaatcctt tgaacgagtg gtgtaacttg 1560 ttaaatgccc acaaattcat gggacgcaat cctttaggag aaaggttggc cactagttat 1620 tggtggttac cgtggctctt agcaacttag catcagaatt tgtcttgaac ttctagtcgt 1680 tgaaaattct cttcatacaa agctaagtct gcttatttgt aggatccata aacatgagat 1740 gataagggtg atgggcctaa gaatgcttga tggaaacatg gtcattggac ttgcttatta 1800 attgaaaaaa ccagccccgt ctctggttag aaccctcatt aggattgtat tgtttcaatt 1860 ctttcagctt gttctggatt ttaaaggctc caatggtttg agatgatagt catggaggtg 1920 ggaaggaatg gacaatacag ttttgaagaa ctgggttatc tcaaatggga aggtgaaatg 1980 tttatgtcag tatttgcttg 2000 <210> 64 <211> 2000 <212> DNA <213> Muskmelon <400> 64 atcttcaccg ttaaatcgcc gtggttgtta gcggcggcga ggagagagag tgctctttct 60 ctgagaactc ctgccatagt agacctaaag gaagaaagtg gtagtgaaac aaggaatggt 120 gaggagggtg agaattgagg aagtggttag ggctttgaag gaacggggaa ttttatttcc 180 gggaagggaa acaacagggg agaacacagc cggagcggtg tggttgtgag aaaatttaag 240 caagcagatg agacgacggc ctggcgccga ggacaggcat atgaatatca cgtggctatg 300 gctatgggaa attgaacgta ggccctttct cattcttata ccaatcttca ttttcttatt 360 ttctagggtt tcttttcttt cttttttcttt tttcctttt cacattttta tatgtcattg 420 aatttcgaag tttggagtta atatgttgga gtcgtgtatc tatttagctt catgggttat 480 aacattattt tggatgatgt atgatattta atctcaattt aagaaggaaa cgagtaacca 540 aaaaatctta taatgaggtt tgtccatctt tttgtatta ttctccactt atcacatttg 600 tttgaaataa ataaataaat aaatgttgtg tcacctcaaa cacaaccata tggttcaaat 660 tgaaatttaa cacttgatgg tccctatgtt ccatacgacc taacaaggtc atcttttgat 720 tgtgaggttc atccaacata aagttgttat aaactaagaa tatttcactt atgagtgttt 780 atgtgcacgt tgttggtata ggccataatt ttcaatcatt taaaactttt attaaccatg 840 atttcacatt atcttgatct ctcccattcg aatatgattt tggttcatct atattcccct 900 tataaactca acgttacgtg cctaccagtt ttcgcttggc tcatccccaa ccatatctt 960 actgtggaat gttttttctc tgataccatt tgtattgttt cacaccttcg aatcatattt 1020 tagaatgttg atacagtacc taatgcatgt gatattcccc tccatttgtt gtgacatggc 1080 agcatttgtt cttacttgtg tttgaattgt tttctaagag aaaaaaaatga tatctccaca 1140 aaccaacgca catcatttta gcatatcatg tgtctcattc acgtggttct taaaaaaaaa 1200 tcaggacatt atccaataag acgtggtcaa gggatgaacc aaatgaaaat taaaagggca 1260 tgtaatggcc gagttcatga atgcgtcata aatgaatcaa tatcacacta aaataagacc 1320 gatcacaagg gtgtgaaagc atagttaaca samaataaa aaaaactaaa agctcatatc 1380 tatgccaaca acatacacat tattttcgat tgcttaatcg tatgaacttt aaagttaaac 1440 gtgtttatt taaagttaaa cgtgcttatc ttaaaacaat cttatgttgg acgacctcca 1500 caattttttc cattacgcat gtgagaaaca cattgaaagg actcgaatta gcatgtagag 1560 aatggtgtag cccccattct ataaaagcaa ctcaagatct gaacatgcat tgaaatttca 1620 ctcttcattc ctgacacata cataaagaga agcaagtacg agaatcatcc tctacttttt 1680 attcacaagt tttaagtcaa atttcaactt gatttgtatg tttcaaaacg acacacctac 1740 tcatttaatc ttgagcgtta cttcaattgt ttttatgttt caaaatgtta aaaaagaaaa 1800 aaaaaaaaag ttcaatagtt ttgtaaattg caaaaaaaga gaattacgag tatgcccctg 1860 tacatttaga agaagcgtaa ggtccatatg ggaatcagaa caatcaatcg acggccacat 1920 ctcacgagac ataaacaggg ggagttggag gaatcgacgg agatcggaat ctggtttagg 1980 gttttagcaa aagaagaaca 2000 <210> 65 <211> 2000 <212> DNA <213> Muskmelon <400> 65 aactcagtga atacgataag aaatttaatt gaagttaaca aactcaaact taaatatttt 60 ttaacacgga caatttaaaa ccaaattcat agtctccttg tatagtgttt agagtgtgtc 120 gcttcattaa acctttctat cgtggaacaa atctcttcta atattttgtc aaaaacctat 180 catcacccaa aatatcatga taattatttg atgaggatca aggcttagag aggaacaagg 240 gaacttttca caagggtgga gagatttagc tattaggttt aactcgttgc ttctaatggt 300 ggatgaataa cgacaaattt taaacaatga acgttatcac gttgaaacta tctacttctc 360 tcaacctact actttatcat aaggtttgaa aagttctatc gaaaatttta aatacataaa 420 acataaaaag gaaaattttc attggagaat tttccatata tgtttaccca caaaactaag 480 gctaattaaa aagctaacct taagactaag gctaaaatgg tatcttatgc tacatttttc 540 agttgctatg ttttgaagca aaagctaatt atttgctaat aatgagatag gcatgtgggt 600 gagtgatgag cttagcctgg cctgcctttg tgtttcttct tattctctta aatatcattg 660 ttcaatcaaa atagtttgt taaatttagc ccatcctcac ttcaacctct tatatttgga 720 ttggccttct ttgttttttg ggcttttgat atttgatgta atggacttca atcatttata 780 gaagccttac cctacagaaa caaacaaaca aaaagaggaa aaaaaaaatg gtgagttggt 840 taataacaac tttctaactc aaccaatata tggtgtgtgt atatatatat atatatcgaa 900 taaaaat gatatgata tgaccacata aaattgttga aagggttgaa attagtgaa 960 ttggactttt aaattttgta gtgtagtggt ttacctatga tgctcgtaat gttatttaat 1020 tttaaatgtg tttttttt ttacaaaaaa aagtctcgcg gtgcaagttc ataagttga 1080 tttaaaaaca atccatca aaatgttc gcttgatatg atcgagtata gagccgaatg 1140 tgtatcaaac ataattca actttaatag agtgaaaat aaatgctacg caacaagt 1200 ttttgtatta gcttcttaaa tgtacatata tactttccg attcaacac ctccaaaata 1260 aaactcaaa gttaaaattt agactcagaa atgagaga aaagaaact aaaaacgaat 1320 tctaagata agcattttca attatagga atgaacaat aattattac aaatagag 1380 aattgtaaaa acgacaat tgacataata cttacaaac atacaaat ttcagattct 1440 atcaatgaca tacactgata tatctttatt agtcatagaa agtctcat tttaaaatc 1500 caatttttg ttatatattg taaatatttta aatttgtttt accatattta aaaattttg 1560 atttatcacc aaaataac catagatttc aaattttgct aataattt ttaccgtttt 1620 atttaccata atttttctat tataaaaaca aaaaaaacaa aaaacagata aaagcgaaga 1680 aaaagtaaga gagcagaaat attttttgat ttaggtttca tttggtaaaa aattgttatt 1740 aaaaaataca aactaatggg aaacaataat aataatttaa tttttttaaa atctaaaaag 1800 aaaatagttg acaacaataa tttaataatt taatacacaa gccagtgtta ttaatctctt 1860 tttttctaac aacgcctttc acgagacatc ctctcaatcc tcgacatcca gtggaaaaac 1920 agtatccctc aaccctcagc tttccccaac cgccctccgt cgttcttctg atcgtcgcca 1980 ccctactccg tcatcggaaa 2000 <210> 66 <211> 2000 <212> DNA <213> Muskmelon <400> 66 catatattta ttatgttcca cttgataacc attttgtttt tgaaaattaa gtttaaagac 60 gacactaatt ccatcttcaa ctttcttctt ttgttatcaa cattcgacca atagtccaga 120 aaaccaatta agttgttgaa aactaaaaaa aaaaaaaatt cttataaagt tgtttttttt 180 ttaaatttgg ttaaaaattt taatcattat acttaaaaaa tatatacacg aatcatagta 240 gaaaattgaa aacaaataaa cttaattcca aatctacttt aaaggctcac tatctgtcaa 300 gaggctttgg tatagttgtc tgtactgatt aagtgtgaga gttcttttaa tatttgtagc 360 tgaccaataa attctttcct ttctttctaa ttttgcttta actccctatc ctattcatac 420 acaataaata tacaccatat tctaattgac aatattgttt ggatttgttt gttttcttta 480 cggtaggcaa gaagttgcct agttgttgtc tgacctcaaa accctttgtt gataagagca 540 aacaaagtct agttttccaa aaaaaaaatc accaactcaa ccaaatcttg agccttttac 600 taatttccat cccaaactaa tatctaatca gtgcttacat gtttgagcct tcaactcaat 660 ttaacatcaa aacatcttgc aaccacacct tgacatgagt atgaaaacaa tataggagag 720 aactttagta ttacattgag ttccattatc attgtacatt ctcaaccaac gaaaccaacc 780 caaaacaaaa tagttttttg taacatatga gattaggtat cgtcctagtt aatgatttta 840 caaagttata tgagtattca tttgttgata tagtttgacc ggatcggaca gttggctaca 900 atggtatatt tctataaact aaggtataca atttttcatg tatgttgttt gatattgttt 960 tattattggc acatgtcttt tgtgtccaat agtaataca aggttgtttc ttatctaaat 1020 aaaataact cttgccagat attgaagtt agacttta tcaaacgta atattaatg 1080 gggatgagaa atattgatt attaggtaa cctaacaata aaatctttaa attgtgttag 1140 atcatttag ttagtcgt tctacactaa aaaaaattaa aacactaaa atcattatta 1200 aaaaatat tcaatactct caaaatgtac tàaacattg aagctcataa aactaatcat 1260 ttttctttg attaaatttc tctctcatat taccagaaa cctaagataa cattaccaac 1320 gattcatacc aaaaaaattt atttacattg aacatatctc aaactagtgt attcaataat 1380 ggttagagta gtagttatat taaggtgcca tgagtttgat atttttcttt tttgcctaaa 1440 ttaggttaag ccgtagctag cttgaacaat gctaagatc ttcttaagg ttcgtagtt 1500 taacgtttat atgataaattt ttattacatc cgaacttgat atttaattt tgtggctctt 1560 atctgtgttt agttttctt attcttttt aacttgtagt atcaatga aagccatttg 1620 caaatgagga caaatgcatc tgcaagatat atattagcca atctcttgat atttttatgc 1680 tctatgagac aatatattct gccatttgcc catcaaatgg ccataatttc tcaagatttt 1740 tccatttcga gtttgtttca atcttctact ccttttgttt ttcctttgtt caattttttg 1800 gacctttgat gaaatatctt cataactcct atgacgtggg caccatccat tggttgtcat 1860 ttgataagaa atatgtgtca atggcacaat tcccattcca tttatatatt atatagttcc 1920 taaagccata tccccatgat ttatatcctt cttcaagctc acaattgaac tttaacatta 1980 cttcttccct acacaaagat 2000 <210> 67 <211> 2000 <212> DNA <213> Muskmelon <400> 67 aaataatttg tggattttat catattatgt accttagact ttgtaaggtt tataacacaa 60 gatgtggaga aatcccatga tgaacatgga cgttattata tcctttgaaa ctaaaaacaa 120 aggaaaaaaa gacaaatggc tgagtataag aaaaagagaa gaaacaacca aaaagctaaa 180<000287�>atgtcatgct ctcatatgta acatatttga attgggattg atttcaagat gtattttaaa 240 ataatttaac acacgataaa ataattctaa caaactcaaa attactctta aacatttact 300 It should be noted that there may be an error in the original text where is misspelled as <000287�> in the provided content. I have translated it as as per the best guess. If this is not correct, please check and correct the original text for a more accurate translation.tgatatcttc gacataatga cttttgtttt aatacaactc aaaacataat taaggttggt 360 tttaaatgac aacaaacgaa aaactcgatt ctaatttcaa tactagtctt tgaaagccct 420 aaggagcgtg gttttgaatt gtatagcaag gtttcgaaaa ttttaatcat caaacaatag 480 gtatattac ctgttcctca agtacagtta attcatagct acttgtcgtg cttcctacga 540 caacattgta agacttgcca cacactaatt gaagccgttg ttgaaggtag ttttccatgt 600 atagcttgaa tcgacggatg accaaagagg ttgaagaagg ttgaaaaat aggggaaggg 660 atatacaaag tttgagagtg agagaggggag agaaatagaa atagaagaga ctgaaaaatg 720 taaaagaaag gatgaaaaaa tgtggggtaa acgcaaattg gatttttata gtagtatttt 780 gaaaatgcta tagaaaggct atgtatagta gtgcttccaa agttctatat gaatgagaca 840 aatcaaaata tatttttttt gattaattaa ccccaaaaag actcataaaa aaatcttata 900 aatcccacta agatattgca tgttatatgt agtaaaattt atcgttcaaa taaaccttaa 960 acataattca aacgaagaaa gtaaaaattt gaatttctta tcttacatca ttcaaccaaa 1020 caatttccat atagagatt aaacttctaa ctttaagaga gagatatcca gcatatgca 1080 cctaaaccaa cagtgacttt gctatatatt tgcacaaat gtgggggaca aagttgtaat 1140 ttcggaatat caatgattta agaaaggta aaatttaaaa ttcggaagct tgacgtggca 1200 acacggaatg gtgatgatat ttccaactcc tcgcgacttt tagagttgg cctcaccac 1260 cgcatatccg cccctttgcc acgtgtcaga ctacacaac ttccacaat ttcctttaag 1320 aacaccaaat tatatcata atattac tattatag atattattg tacaatttt 1380 cctactgagt tagatagata gatagacttg tcaattact ataagtcca aagtcaattt 1440 actcaacata gatacaatc taatttgagt gtgaaataca tattcaatc aaaattatta 1500 ttaagaggaa aactgattt gctttctca ttaaatat atattttga aaagaaaca 1560 cacatgtatt atggttttca atatatttac ttcttagtc acctaatctc aaactaattt 1620 ttgaagaat aatatata tattatcatt tttttct tggttatgat attggtatag 1680 aatcaacaa actacaagtg tcctctcctc tgctatcgat ggggattaga ctctaaactt 1740 gtttagcgat tagtaattat atattagaaa aagtttatgt taatgtggac ccgacaatct 1800 caccaatagg ctcttcactt caccaacccc cgacccattc cctctaataa ttcgacacgg 1860 ctcatccccg gttcgaaccg ggccgacgtc ttcctattta acacacctcc atttcctctt 1920 ccctccgcaa cacaaacaga gacctcaccg gaaaatcaag ttaaagcaaa accaaagaga 1980 agcttcatca ctctccggaa 2000 <210> 68 <211> 2000 <212> DNA <213> Muskmelon <400> 68 taatagttgc aggtcttgtt taaaatacta atctaggtgt gtaaaacata gaaagtttaa 60 tgtggaattt cttatgagaa cgattaaggc tggtgaatct cgcttggtta aatttgaagt 120 agtttcactt cgatggagac tagacgttta ggcattcgta atttcagaga caacataacg 180 aggctacgtt gggaaaatag ttatgtcatt ttatcataac tgcatacttt gtggtaagga 240 tcatactgat tagtaagtac ggtttccact ctatagtgtt tgagtcaact tctgtgcccc 300 tttactaatc tcacgagaga atccgcctgg tcctgtaaca tttgggtgtc aaagaaactt 360 gtaggaaaga ttccgaccac catggattga atcataagtc aagggccatt agtaaaaacc 420 tctaacttgc tcttgcttta aattttcctc tattctcctt attcgttaag cattgggtgt 480 gggtgctata ctaacttttg tgggttgtta atggcctttg tttctgtaga tagtaaggac 540 ttctactgta aacttgcttt ttgtttgcac tttctcactc tttcattttg ttaaaaaata 600 660 720 ctaccatcca accttcttc atacacagta ctctcggccc ctgcatcacc attcgataaa 780 aatccaatat gtcaacagaa cctctcaggc aattgaaccg gaataaatta gtgcagcgtt 840 gagtgcttac ctcgggcaat tggagagagc agcgtgaatg cggaaggttg your area 900 ggaatcgaat tgctggagca gcagccctga tgcaggtgtt cggatccata attcccaaat 960 ccatatccgt tttcgtgaag aaatgttgag gaaaaattca ttatgcgttc agtttatacc 1020 attggagagt gggaaagttc gtattgtttt gctaatttcg tcgattctca ggtcttggag 1080 taaaaacgtt gtacccgcca cttcccattg ggccattgtc caatattgtt tgggttggc 1140 gggtggatga cccaaatttt ggggaagata tgagatttgt ccaactctgt tatcaaatat 1200 gaccaaatga acaaaatatt gacttttttt tttctatatt tttttgaatg aagtataagt 1260 agttgtttta ttttgtttat tttaactcaa aattaccaaa tttggatttc acaaacataa 1320 aatagatttt atacttttta taattcaatc gaaagttgat cgtatatgaa aagaacaatg 1380 aataaagaaa gaaatgtaaa atttatatca acttaattaa aacctcgcaa tacaaaaatc 1440 gagtgaaata gagggtggag gatgagagga agagggagaa gacatccata ccctccatgg 1500 acatgggtag atgtatgggt tgggttgggt tgggttgaat tgggtcaacc catccactcg 1560 gttcatatag acagcattcg ttttataatt tatccaaaat aaaatataat taaaagaaga 1620 aaataaaaga aaaacgaaat ctccaattcg cgtaggaaat taaaaaggaa gagttaattc 1680 aattcgattc tctcccatct tcatcataat tttgcgaaag gatcgtaagt tgtatacttc 1740 tctccttgct gcttttcgaa tcgggataag aatattttct ttttgtcttc cccattccta 1800 ctcttcaaaa ttctctgcat tttctaccca tcacttttac ttcaaccatt tttgttgttg 1860 ggagttccat attttgattt cctctacaac gcctaaactc ttcttcttct tcttcttctt 1920 cttcttcttg gagtgatttt tcagttcaat tttggggatt tcatctattt ctttgatctg 1980 cagcgttgct ggaagttgcg 2000 <210> 69 <211> 2000 <212> DNA <213> Muskmelon <400> 69 agtttattct gttgtagcaa ttcaaagcag tgtgatccag atgagtacat atttggtagt 60 agactcaatt atcattttat ggttgaaaac cacatcctct tctgtcattg ttcatattat 120[[ID=二十一]] [[ID=二十二]]tacgaggaaa aaagccacgt cctctttcaa aatttcttcc acaaactctt tcttaaaagg 180[[ID=二十三]] [[ID=二十四]]aggaattaga gtttgaaaga ctaattagat cgagaattat tcatattcag attggtacct 240[[ID=二十五]] [[ID=二十六]]aattgagtag caaccatcgc agtaggttga agagaaaggt cctcttgttt acgatgtttt 300[[ID=二十七]] [[ID=二十八]]gcaagagcaa attcttcaaa tttcaagaga gtatgaagtt cttcaaagat tactgattgt 360[[ID=二十九]] [[ID=三十]]gaacgagtgc gcatagagat gtgaaaatat tgtattcatc tagaaatcaa atgagagtat 420[[ID=三十一]] It should be noted that there may be some inaccuracies in the translation of the specific biological content. It is recommended to double-check with relevant biological knowledge and professional terms.agatcaacaa atcttcatcg tttactattg aaacacacat ttgcaagttt atctttgatt 480 tttttgcccc tcctcatgta tgaattaata gattcatcaa cttctttgaa atcgattgaa 540 gattagttt gagattaaca atatttgatc gtaaatttga gtagtgattt tcaagtgcga 600 cccagacttc ctttgatgaa gtacaaccaa caattagggt tttacagac atagtagcac 660 tgatcaaggt cataaaagct tgatctttag caatttaatc tttatatata aaagattcaa 720 cattgtcgtc gattgatttt gtattgtaga tgaactagtg gtcgaagaaa tcaaaatcga 780 ttttgaaatc aaaatcgatt ttgctagagc tgtacttatg tcatcaacaa atccatataa 840 atccatatag cttgtgtgct ctcaaaatag tggagaattg gaatttctaa gaaacataat 900 ttgttgattc gagtctgata aatatgaggt acatatattt gtgaggaaga tcaaaaaatt 960 gatttcttt gttcaagaag actcagcgga agccattgat ggagagaaca aaaatcggag 1020 gggatggtta atcatgggtc tttgatctgc tctaatacca tgtgatcttt accaagttgt 1080 gaaaaaataa tctctcattt tctcattaat ttacaataat agaatatggg tatctattac 1140 aacccaattt acagaggaaa tactagctga ttacaacaga atcagtgcca aatcaattat 1200 taaaactaat actcaacact aattaccaaa gaattagtgg tttttttacc acgaatttat 1260 ggggtaaaaa aagtgaactt ttaccaaatt agtaaaataa aaaaagaaag aaaaaaaaac 1320 gtaatattca aatggatggt gaggcatgaa gaagagtagc ctaaagtaca tgaagagcta 1380 aaagacttat tatcttccat tggtcccatt gaagaccaca aagaaaatat cagtcctttt 1440 tctctttaga gacacaaacc caaagtagaa agaatctttc acaagaatta ggaatttaat 1500 gcaatttttc tttttaaaaa aaatctccaa tttctatct cattatccac cctttccact 1560 ctaaacttca ctacaatttg atgaaatctg tttccaccaa tcagattgca ccaaattcca 1620 tcaaaaacgc cccatcagat aattatggat gtcttcttct tcctctcttc tttcgtggct 1680 gaaattgaag ctcaactcaa aaatacattt cattttcaaa attccctgat gacccaattc 1740 gccacgtgtc ccttccactc accactaccc acacaaaaca actgcttctc ttcctcttcc 1800 tcttcttctc cattaaattc ccagacccat ccctctgcaa cttcgaatgc aacagaaaga 1860 aaacggacca aaaatccctt gaggaatttc tcatttttga agcataattc aaagattaaa 1920 cccgtattaa ccctcttcat cttaccagag gtttgattta ttgatcgaat tgttttattg 1980 gttttttttc aaggtcacca 2000 <210> 70 <211> 2000 <212> DNA <213> Muskmelon <400> 70 gcatcttatg gatgtagtca caacatattt atatggattt tctgataatg atatttatat 60 gaaagtccca aaaggattta agatacctaa aacatataaa tcaaattccc ataaactatg 120 ttcaataaag ttacagagat cattgtatgg attgaaaaaa tcatgacgaa tgtgatacaa 180 tcgcctgagt gaatatttag ttaaaaaaat aatatcaata taattcaata tgtccatgcg 240 tttttataaa gaaatcaccg tcaggatttg ctattataac tgtatatgtt gatgatttaa 300 atataattga aattttgaag agttttcaaa ggcaatagaa tattaagaaa gaatttgaga 360 tgaaagatct cagaaaaata aaattttgtc ttgattttca aatcgagcat ctagtaaaag 420 ggatatttgt tcatcaatta acttatacag agaaaatttt aaaaagattt tatatagata 480 aaacacattc attgaacatt ctaatgcaag ttcattcatt aaatgtgaag aagatattt 540 ttcgacgtcg agatgataat gagaactcc ttagtccaga agtaccatac cttaatacaa 600 ttggtgcact tattttgtca ataatcaaga ccagatattg cattttctat aaatttatta 660 gctagattca gttctccaac aaaaacacat tggaatgaag ttaaacat acttcgttat 720 tttcgaggaa cattaatt aagattttt tattcaata aatcaattt taacctagtt 780 agttttgcat attcttgatt ttatctgat ccacataaat ctagatctca aacaggttat 840 ctattcacat gtggaggaac tgctatatct taacgatcag tgaacaat taccataca 900 gtcaactctt caaccgtgc tgaaatttt acaatcttg aggcattcat gaggctagcg 960 gagaatgaat atggttaagg tcgatgactc aacacattcg aaaattatgt ggtttgtctt 1020 ctagtaact ccttccaaca acattatacg agacaacac aacttgtata gctcaataa 1080 aatgaggtta tattaaaagt gatagacaaacacatctc accgaagtttt ttctatactc 1140 atgatcttga agaaaatggt gatcacag tacaaaaat ttgttcaaa gataatttgg 1200 tagattatt tacaaatta tacctactg caaccttga aaaatttgtg cacaacattg 1260 gaacgcgacg acttagatat ctcaagtaat gttacatctt acttgccaag ttaactatac 1320 atagtgacat ttggtggagt tgtaagaaac actaatattg gagaaaaatc gaagaaatt 1380 ggaaaatatg gagaattgaa ttttttag atttttctta ttttctatt ttaggttttcc 1440 gtattctgat tatgcctcat ttcacaca ttaatactt taatagatg atttctttggg 1500 ttaagggaaaatcatttt tttagagttg cacgtacaa atattatca windowscg 1560 attataa accaattcac cgtcaaccta acctaggtag agttttgagtt aaatgttaaa 1620 agatatcca cccctcaa ttgtaatccc actataaa tcagcaacct aaagtttttt 1680 ttaaaaact aaaaagaga gcaatatt tttttacta tttttttt aaagagtgga 1740 tttatttatt aaatttaaaa atgaaaagaa gaaatttgt tagttttgggt atccgaaaa 1800 cccgattatt tgggcccgag aaccgacgt ttgttatt gttcctcacg gcaataagta 1860 atggcgtgaa tcgaccgcgt gcgcttcaag cttactagac atttttat cctccgatta 1920 gaaaccctaa ttcagattct ccgtattacc caccctggaa catctttgaa acgcgaaaag 1980 gtgacccgaa gaaacttgaa 2000 <210> 71 <211> 2000 <212> DNA <213> Muskmelon <400> 71 taataaagtc gatgatatga attaattaga cggatgggtt atactatagt tattttattg 60 tcttttatag agaatttaac attggtagcg gggaaaatcc gatagatatt ggtgaagagg 120 aattcgttgg tggatgtaaa ggaaagttag tttcgccttg gcacaacgaa gggtttgaat 180 ggaaaatcat gataagttcg actgcctgtt caactaaacg aaagatacca agtcaaacct 240[[ID=​​​​​​​​​​ttttttaat ttgaaggg gtgggctcgt gtggcagatg aggcctgtca taattagcct 600 taccttaaat aattgggccg gttctttggg aaatatcggc ccaacctaac ttttcatggg 660 ctcaaatgat gctttatcta atacccatac tttccattac ctttgtatat tgaattagaa 720 tgatagaaaa acatactaca cagttgagtt aggatataaa taaatgcatt gaactatgta 780 ttacatagtt gagaaaaatg agaatgaagt tttgtctttt gaatatatat tctgtgaaag 840 ttagatgtat atagaaatga tgatacttcg gcgtttgttg aagattgagt ggggtgtcaa 900 cctaatcata gttggtttaa gaaaagtttt aattataaga taaccgtttt aagtgactta 960 tgccatattt tgattgcagg ttcacaatga aatgttttaa tttggtgatt agactttgac 1020 aatgtggtaa tttatgttaa gtgagttgtt gtctcgttta ccttgatcat ttgtctctac 1080 tcatttctca ttttgttca tcccttgtta tatggcatcc attgttgttg tatttgtcat 1140 tgttcacatt cgatgcttaa ctaggtaaga acaacatttt cattttagaa ttggaacgat 1200 agaaattcat aagttttatt tttgaggcac ttggttcatt ttaatcatag aacattagtc 1260 cacaatcgtt tggaataat tacactcta tcaatcgtg gaaccttga caaccttac 1320 aaaaaaaaggaaaaaaaaaaaaaaaaaaaaacaact 1380 aattagccca ttgacaaata ttaccacgtt attaaagttc atttaatca tcgtgtcaat 1440 tatcaacctt ataggtcaa taccatttat atttattttc aaattcatt atgaaacaa 1500 gactcaaaaaaaaaaaacc caatttga gtttagaata taataatttc 1560 atgttagac ttggacag atttgtacgt atatgttaaa ttaaaaattt atcaagta 1620 taaataatg atttggagtg gcaagaaat atttgccaaa atttcatag aaaaggaag 1680 aaaaaaaa gtgtattgg caaaaaaa cccaattcca tggggag aaaatttgag 1740 tcctcaaaaa aggatttcag atagggacc aaccaatcaa aacgaggac gtctccacgt 1800 gtcgctacaa gaggccatct ttccaaatg agatcgcgga taacaagcc tttctgagc 1860 atagaaaaat ggcgaatttt aaaaaaga aaatctcag taaagtcatc agctacagct 1920 gctcttgac ggccacttga ttcactatt cccctcttt ccggcgctga ttctagtgtg 1980 gttgaacttt ctgcaaagaa 2000 <210> 72 <211> 2000 <212> DNA <213> Muskmelon <400> 72 attacttgaa cttaatccac atttatgtct ttatataaag ttcgaatact cataatatag 60 ccaagaaacc ttgtttattg gattttgagt tttatcataa gcaaatctct tatccaataa 120 caaattatta aacaacactt caacaataac tttattcaac aatatattag tttaacattc 180 acaaatcacg agtattagaa cataaaacgc aacaaagaat ggactaaggt actatattct 240 aacttaggtt gtttaggatt tccatatgtc aatgcttttg tgatttttga actagatttt 300 cttgttagat taattcaatt ctatttttaa atggcttaat atcttatttt cggatgcttg 360 gggattgcta gactaccgct ttgttgaagc aataagttaa atttgtttgt tacaggtatt 420 gatcaatcta acatagaatt gaatttgtat gaatatttag ttagacgctt gaaaactaat 480 tattctacca atgagccgta gatcttaatg caattgttat taatttgaac tttgtatgct 540 tctcatcgat taaatttata tcaagtagtt aattaggaca aatctattgg ctttttcatt 600 taatttgtt aagtaaagc aacttagaat ttgaaaatg atgaacccat gatccaatac 660 attgaaagg aattttgttt aactcaact aggattctc tcacattgat ttcgtataat 720 ttaactttt caatttatat caatcccccc agggtgaaa aaatttgttt gagaattca 780 tgtgctttct aaatctgatc tagacttgcc actaaaatta acttttgata tgtaatttgg 840 ttaatattt gattcggatt tcgacgacaa acaatgatc aatgtggtat taaattctga 900 tctccatgta agaatttac acatttcat aagttcaatg ttgacacaaa gagagtaaga 960 gcattttaaa aaaaaagata cttttaatct ttctaaaa aacaccaaaa tgccattatg 1020 taaatgtaac ctaaataata aacatttaa cttagaatttc atgcaattag gctttgtac 1080 ggacattgaa ttgattatta aaatcagtag ttatagaccg tgagttataa tggtttgtat 1140 Taagcata attatttta atttgatcg tatagcatg tatttgagat ataattaat 1200 ttagttttgggg tggcaatag taacagtaa agcaaataat aaaaaatga atttaaata 1260 gtaagatttg taacaatga ttaatactat aaaacgtg gttttaaaat aacgttgatc 1320 gtagctaatt gaacattatt tattgtaaaa tgagtgtttt ttatatttg gagcctcaa 1380 cttcgggtgg atcaccaca tataatcata ttcaattta aaattttatt tttttatta 1440 atataaata ttgattgtta atagatgctc attatgggcc atctgtcact cccctccgtgc 1500 atatcctacc tgaaacatca tatatcttta acatgtcca ttgccatgtg tcactatttt 1560 tacatcccat ccacttgaca atcatgttga agatgcctac tttttagg atcatgtaat 1620 ctatctcatg cttgtcaaat tgttcgataa tagtgttaca aaaaatttag taattattat 1680 tattatattt cttcgatatt tatgcttcat atgccattgt gctctccatt tttaccatac 1740 ttaaaaaaat ttcttattat aaatttttc aaaaaaaat ttactatata gtcatcatct 1800 ttattaaat taaaattgag aacctgattatt ttttgattatt aatttaa atttgaatt 1860 aatccacttt aaaattatta atatttatt cgaatttgggg ccttaaggaa gagatacgga 1920 aacaaaccct agatcccatc tatatataaa tcgccacaa accctacctt tctctcagtt 1980 tctcgtttta gccggcaaaa 2000 <210> 73 <211> 2000 <212> DNA <213> Muskmelon <400> 73 tgaaaaacta aattaaattg tccttacatg tgtataaaag aaaccttcgg acatttgatc 60 tgagaactat gttaaataat aagatccaaa aaactgaacc ccaacatctt cgaaatcgat 120 ttgatttcaa ttcttaagat aagctacatt caagttacct agatgatcta agaaactaat 180 gattggacaa agttagaaac tcccaataaa ccaatgatct tcaaagcact ctacgatcaa 240 gacagattaa tttttagttt gaatgctttg aacactcgtg cattctatca caagaacaaa 3​​​​​​​​​​​​​ctaattaaat aatgtgtgat gccccactcc ctaaaacagt gggtttggat cgattaatca 720 actaaaactg accacaaac aatattcttc tacaacccca ttgatttttt taatcattaa 780 gtgccgattc aaagaaacaa taaacaaaag aagttgaaaa gattgagact tttaaattaa 840 atctgcaaga ttctctccaa actcatgttg tattcaagtg tttaaagctt aaaatatcag 900 taattatgtg ttatttaacg gtgaaaccaa tcaaatcaag caagattctt caatattcaa 960 ttccaaatcc tcaagtttcc atgaaaactt cataacgcct ttatccctcg aaagccaaaa 1020 ttcaatttcc tccattcatc ttgcagccct atctactttc caaaagccaa caaataccct 1080 tttaagcagt agcctttgt ttggttgtag taggatcttt gtttctcttc cattttaaca 1140 caagccacag gagaatctct atctctatcc tgcaaccttc atccccacat tgttcttcct 1200 ccattatcgg aaaaacccag tacagggttt gctttccggc cactatccgg ttgttctttg 1260 taagtttttt gggttttcat tatctgggtt tgtggctgct tgtggattca gggtaatgtg 1320 gccatgtttt atagtccaca gccttttttt cttcttttga catgggatta tttctgattc 1380 tatttgtcta ttgttacttt gtgctttttc tggtttgttc ttgtggtcat catttcttat 1440 gcttggaagt tcgaacatga atcaattcaa caactaagtt gagagtgttc gactctctca 1500 tctcattgac cctgatggta tatcttggct tggaagttag aacatgaatc aattgaacag 1560 cttacttgag actcgagagt gttcaactct ttcatctcat tgaccctgat gatatatctt 1620 ggctttggag ttatgaacta tgagagcttg gaggatgaac taaaaagaag ggactatttt 1680 ttgagatgga tatttagttt tagtaattta gctttttttt tttagtacat agtacattaa 1740 ctttgttcgc gaggaaatag tggtcttgtt gacgagcatt tcttaaacaa tgtagttttt 1800 gtctcatctc tttaaaagtt tatggagggg caaacaagtg agatcaatag ttatagtatt 1860 tcaatctata actttggaac agctgatttt taacttttcc tttgtctttt ttttattata 1920 gaacacatta gagtgcgtta gattcttcag ttctgagatt ttgatctttg agtgctctct 1980 tttagcagta gaggcaaaca 2000 <210> 74 <211> 2000 <212> DNA <213> Muskmelon <400> 74 60 ttgtttgaag aaatgtttca ttgcaactga tatttgtcat tgatgtacaa atggctttgt 120 aactctccac tttttctaat ctaaccattt acatacaaaa tatctacgat aactaaaat 180 gaataaagaa attttttttg tcaaaaactg tggggagaat tgctccttgt tctcaaatca 240 300 tcaaacctcc agttgggtgg ctgaatccaa actcttcttc agccttgttg agcaagtcta 360 tgaatgaagg ctgactcaag tacgatattg gaacgaaaaa ccgctttctg tcggtttctc 420 ccacgtacac tggaatgtgg cctttgggaa caatggactg acatcttgct gagacagact 480 gcatcttgag aacttgcttg gcagcggaaa gaagaccga aggcaaacga attcccatgg 540 600 660 gaaggtattg tgacatatga atagaagatg gaaaccaag aaagttgggt tcatcaatgg 720 ctcacatggg tgctccatt ggttaaggta cattcatttt ctcattggca ccaatttctg 780 gtaagatggc cccatatgtc ataatacgtg aagtcatatt gatctaaca aaatgggaca 840 caaaaattgt aactattca attagcatta aaatcatgtc aagaaacta cattaaatat 900 agatatatta gttaatgatg taatatagt ttcatgtgag atcaaactac gatttttttt 960 tataaataat gttacttta aaaaaatgtc aaaaatatgg tagagaaaa gctattacaa 1020 aaagttaagt catctactcg gttcataatg cgttatcgtg gatcgggtac acgacaaggc 1080 aatgagaca tagaccagt ctatgacttc gatgtaaaat gtgggttttt cctattact 1140 cgtaaaaaaa tattttgaa acttttctt ttacaaac ttaaattttg gttaattata 1200 tataaata ccatctttac ttcttatta tccaaaaca ttttaccatat attattatat 1260 ttcaata aataata taaatatttt agataaaaatcaatctta 1320 tatatttaa atacacta agctaattta attackacatt ctgaaaattt taatatatt 1380 tctatctaat ttaagatttt attatattt ctattaattt taaaatttta atggaaattt 1440 aaattgtaaa taagaataag agtacaaact tactattttt atttcatttt taatttataa 1500 acttcatctc ttttttcata tatttttaag aaatccaacc ttatatttcg aaatttattt 1560 aaaaaaatta taaaattttt taaactatat ataaataaaa attgtaattt ttgaaataat 1620 ttattaattc ttacaacaaa cttataataa taacaataat aataataata atgagggtac 1680 tcgattctca aaaaaaccga accgatcaaa caacgttaga tcaccaacac agaagtaggg 1740 tttttcatcg gcacataaaa accctcactt cttcttcata aaaaccctca cttcttcttg 1800 acctaattcg cgccgttgat ctccggttcg atcggtttct acgctgtaat ctcaagctat 1860 ctcctacctt atccttccct ctctttttct tcttcttctt cgtatatgca tatcttcaaa 1920 tttgctgctt tttttgtctg attattcatc tgggtttgtt tgcaacagga aggaggaaga 1980 atttcaaatc aagaagaaaa 2000 <210> 75 <211> 2000 <212> DNA <213> Muskmelon <400> 75 tttattaatc tgaatcattc tgtttcttct gagagtttta ttccttttaa gattctaatt 60 ttattttgga tagttgaatt ttggtgtgct ctctttgccc cttctttatt atacattcct 120 ttatcttaaa aaagccaaaa agttaaaaaa caaaaactaa tcaaaattgt aacatttaca 180 attttatgag catgacattt aaaatatcga ttttgaagtt aagacgttgt attctcacca 240 tcggtttta tctcttccca ttccattaga gtgataggct ttatctttca tcactgtcaa 300 aattcatcca acgtccaaga tctcttctgc aaagagttac ccacaattct ctcagactca 360 ttggcccacc ggataccgag tggatggata gaacctccaa gattgcgaga gcaaaagctc 420 agccaaaact tgcacaaact cacccatggc ttccctctct tgtactacct ccattaatct 480 caccccaaga tccttcaatt ctcgccccca ttcaaattag cttcccattt tcttggtctt 540 cagtccaacc ttcgatggct ctcacccctc tccattggac cctccaatgg gtctagagca 600 acttgctggt tcaatttaag gcaaaatgcc gagggtgcag gcatttatgg cagccagtcc 660 cgagatgatt tcaacagaga tgatgttgag caggttcttt tactaatttc tctcttcttt 720 ctttgtattt tgttttgtga ctttgattgt tgaagagtgg tgtctttgtgt ttaattgctg 780 gtttgggctg attcttatgg gtttggagtt gaaattgttc ttaccctctg gctgttctgt 840 tttctttaa gtattgtgaa ttttcaatgg ctcctttagt gaagatagat gaagaaattt 900 aaattagtaa ttttcgtac cgatgactct cttccagtgg tgttaatgtc aaactaacct 960 tttctttacg tcataaagca cttaatcggt tggaactcag tagacgtctc actcatgttt 1020 gtagccctaa cctaatgcca tggcaatcga aatttatatc gtatccctat tgcgattatt 1080 aaacatcacc ataggtgaga cattcctaac gtgatatact gagttctaga tggttaagtg 1140 ctctgacatt tcacattaac gcctcatccg cactggttag tcgaaagaag aaggtgtttc 1200 tgtatgaga ttgtgagaaa ggacctcctt aaacattata accaacctca taacttgtgc 1260 atttgtgtat caaactctgc tttcacataa agaaactaaa acaaggtatc acattgccgt 1320 tatgaaaagt gcatagaact tcctgcttcc ctcaaacaaa acttgcaaat attactgatt 1380 ggccttagcc tttaggtaag ggaagaatca aaagtattcc ttcatccttc tgctttaaaa 1440 atgtgctaaa tgacgttgtc catagtttaa aaactcgacc aaatcgcatt tgtcttacag 1500 tctctcaacc ctttttaagc actctcagag tcaatccaaa tagattccta gttcctaata 1560 tgtaacaaga agagtgatac tatgaaaacc cacaaaaaac ccacaaacat gtgacttgag 1620 ttaagatgac tcccaatccc actgtatcaa gcttttcaaa tagaggaatc acgatgagat 1680 gaacaataat atcccaacgt gctgctatcc caaattagat acagaagtct acttgtggtg 1740 ttcttaatcc aataattcat tatgaaattc ttatataatt tcttaatgag tatcttagaa 1800 ttaatgttac aacttatctc ttattctata tgatagaatc ttaacataag tattcatatt 1860 aagagcaaga ttatgttgat acttctcgaa tcataccaaa aacttggaac catgacatta 1920 acttcattcg tggaaacaag ttttgaagga aaaagaagga ttgacaaatg aacgttatgg 1980 ttgtgcagta ttttaactac 2000 <210> 76 <211> 2000 <212> DNA <213> Melon <400> 76 atctaaaact gcatttttta ctacatacag attcaccttt aggtgctggg gcttccccta 60 tttcatttta tcaatgaaat gtttcttatc tagaaataaa aagaactaca tacagattca 120 caccactgca gaaggtca aaaacatt caccactagt aagcaatatt 180 ttgtgaaact tatgtgatgc acttaatata tgaacgatg ccccttgttc tctcaagtc 240 agatctcttt tttcctaca attgagaaa gtggaaataa gttaattacc acggccacgc 300 aaatctcc tgatggcctc caatgaaccc cccaacata atgctgtagg gatgtcttc 360 ttgcaatcct tcaagacgca caatgtgaga catgcaaat atttaaaagt gatacttca 420 atatagca aagaatcaa atattaca aaaatatag caagtttca tattttatca 480 attatacaca ctgatcgaca tattttgtaa atattttca tagttttgac atctacaata 540 attagttgag attttgtagt cacaggatc cagatttgtg tgttgaagt tgaaacccat 600 gataagataa atcccggtt aaatatttca tttcattct taagttttg aaaaggaat 660 agcttggtaa gctacattcc gcatggtaaa caagcataca acttttgtttt caagaacca 720 aactacta aaaaag agtattgat ttaatccaag ttacaatga caattggta 780 atatttatag gatttagtg agatataca atcaagttcc aaagatgtt atattaca 840 ctatgagcat tcatcttgtt actaccacca agaaaaagta gcggttttcc aatctctgtc 900 aagtatccat ttgagttatg atttcatatt caagactgtc acaaaaattt cattaaaagg 960 tgcaagtgca acatttcctt aagaaaagga taactgagag atcaatgact ggaattcaca 1020 agttaaaatg aacacaactt cagacaatca caagctaata cctcaaacg gtccaatag 1080 ttttctgca cactgtcaac aagcgaatcc tttgggcca tcaccaagc agctcggtcc 1140 cgctgttacc aagaaacagc aatttcagca agaacaaat atagaatcc tccaagaaaa 1200 aaacaaac aaataagttc gaggcacca catatcagaa agcttatgga ggaggtacatg 1260 tagtacaaac gctcttgcca tttagtttta cttgttaaaa gtgatttgct cagataaac 1320 ataacccaag cagaatccga acatatgaac catgaatta ataacccca tcacagaaag 1380 acaagtaata ctcccagaat tgtacttat acagacgacc actacaattt agccacacaa 1440 tatcaccatg ttctccaa atatatttaa aaaaaaaaa aaaaaccctc ctattgttgc 1500 ggttaacaca atagatcaa aaagaaaaaaaaacta aaggaca aggtgttaa 1560 atttggttta cctgtttacg cttaagatca cgatcaaaaa ccttaacctt tgagctgtgc 1620 attccatcac cgattcttcc gtcataatta tcatcaccag tagagaaaga acaacaagga 1680 attgaatttg gaaaatctcg ccatcttgca cttctcaaca ctgagaacga tcttatgatc 1740 gtagacggcg acagccctct catttcacag tcaccgattg aacctcgccg gagagacgga 1800 gggaaatttt gtaaattttt aatgggcctg ggccgtaaag tcgtgtccaa acactcctta 1860 aacggaccaa aaccggcgta gaaatgaaac tatccagata agggacgtgc tatacattta 1920 tccaaacgag gtctcttatc gtatcttgta caagttcgtt gcttttcacg gctgtctcta 1980 gaattttggg ttgggcgaaa 2000 <210> 77 <211> 2000 <212> DNA <213> Muskmelon <220> <221> misc_feature <222> (1)..(2000) <223> n = g, a, t or c. <220> <221> uncertain <222> (1)..(2000) <223> uncertain at all n positions <400> 77 aaacctactc tgtaaatgaa ggtttacatc tcttaaggca gtaccatttc tgccattact 60 tctaccattc ccacgaaacc acctctttc tctttcattc tccccgtcag gtatgcattt 120 ctcatctcta agtccgccca ctgttttccg actgattttt cgatttttaa ttagtgagtc 180 ggttttattg tttcttattc taagctttct tttactcttt atatttttag atattaatt 240 tcggatccat tcttcccatc atgcccaatc caaagactgt cgaaagtttt gattgttggt 300 aatgggacat taggtctggg gtttctgttt ttcttatcct ataaattggt tatccttcgt 360 ttcctctatt ttgactttat tccgtagtta ggttagaaga agaaactact gaataatgtt 420 tactatacaa acacctcaaa atagccaagc ctgtcgaaac acatttagct gataagctag 480 ggatgaagag atcaagagat ggttagctca gctgtattgc atctcatggg ggacgggtga 540 aacgaaccag agaagtaata tacacgtttt ttttttaaaa aaaaaccga ataatttacc 600 tgttcttgct acaattacac cgataagttt tcaacttgag caattacacc gtctaatttg 660 cattgctgaa gaaattggtc tgttccatta ccactgttga ttaaaaagtt ctacttgtca 720 gcacagcatg tccatgtgcc cagatagttc ttgatctttg gaaaaagtgc tatgtttgca 780 tgcttcggta agatgtgagg ttaaaatgag gaggacataa tgttggcata gggaggtcaa aatgtgttaa ttgagagaaa aaatgtggtg gatattggag aggagacatg gagagaga gaaagagatg aggagggagg ggtgaaggta aagggaata gacatacaga aataagaac tgtgcgagta atgtgttgcg ataagtgaaa gagagaaagc aagagaaaca gtggtagaaa attgaagtat agagagagat gtagagaggg aaaatatgga gaactacaag ataaaatatc tttattcttt ctctatctaa gtatttatct ctttagaagt tatctctctt tgttttctgag tttaccccta gtattttctt ttttctttct caagcccttc ctctctaaca caatttctct ctctctcttc tccctctctc tctgtatctg gctgtggcac tttttttgac ctcttccttt 1260 1320. ctgtctttat ctcctttga gacattttga ttttcctaca cccctcaatt ggtcttctac tcaaactcat ctacttgtta ttatattaaa tgcatgaaat cctaatattt tagctg gagactcatt gtgcgtgcat ctgcttgctt gtagaaagtt ttaaattgaa aggcaagccg aaggggccta attattcagg ccaggacaat gatgttggtt ttagtttttt gtttttgaaa 1500 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnttttga aactaatttt 1560 tttcttagtt ttcaagactt ggcttggcat ttaaaaacat tggtagaaaa tggataacaa 1620 aaccaagaaa cttacatgtg gaagtagtat ttataaagct tacttatgtg tggaagtagt 1680 gtttagaagc ttaattttta aaagtctata accatatggt catcagtaga gtctcatgca 1740 acttatgttg tgacagtggt gtaattgttc taattaaaaa ttttcgggta caaatgtaaa 1800 aaacattatc gaacagtggt ggtttgtgaa atatgcatta actttttgaa aatttgatgt 1860 gtcatcatat tcattccatg ccgtgccttg tttccctccc agctccttat ccatgctaat 1920 tagattcaga ccattatccc tttggaacag ctatgcttaa ctctgttctt ttctccctct 1980 gtacaacagt atatcaaaaa 2000 <210> 78 <211> 2000 <212> DNA <213> Muskmelon <400> 78 tagcttgtta attcttgtgt tgaagacgtg tttcaacaaa tctgatgggg tattcatctt 60 aagtgtccac tgaagaatgg gggttctgtg gcagatctgt atgttatgta gtgaaaacaa 120 atctgtaaag tttttttta cttcgaattt aacgttgctt aagcttctgt gtacagtttt 180 atcactgcct cgaggttatg attattattg gattaaatta caatttagtt tacgtttacc 240 ttggaactgt gtatttcttt tgattgctca acttttctcg gggatttttc aagaattgta 300 tttttaaaaat tttaatttat ttggaacatt aagaagttgg ttatttacag atgagatata 360 acactgtgat tggggtggaa aataaacaca gcttcaaaca cggagtgaga tatagttaat 420 tacattacat agtactagag attatataaa tcactccact cacatgagtt ttcatcttaa 480 aagattggaa tttacatctt aacagatgca atcttttaat gtagagttct taacgtgttc 540 tcttacggtt gtatcttttc gttttcatta ttctttggtc aaatcaaaat tagactttat 600 agtttttaat gaaatattgg acacactacg attcatcaaa gtaacccatg atcttataaa 660 gttgtgaaat gtatgtatat tgtctttgat caaactttac gtttaattat atcttgaatt 720 tataattttg tatttaagag atgaatgaat tttagaaaat tctaaagttc ctaggccaaa 780 gttgttatag aagggtaaag aatgctttaa atcatttat ccataatcat tagtttata 840 atttttattc ttcgtacta ttttttaaca aaaaaaaaaa aagttatgca tctcttaaat 900 actatctttt aaaagggaaa ttttcataaa taaataaaaa aagacgatag tatacacata 960 aaaaaactc aaatgattta tagagagttt gatgaatttt gctggattta taaatagttt 1020 agaaaaataa gtattaacct aaaattttgc ctatatctca atggccttct atgtctatgt 1080 tatttcttaa ctaaaatcga aggattatag gcttatggat tggcttaagc taaaaaatgt 1140 cggtccaaat agttgagatg tcaaacctta aaagtactac gattatgtga ttttcacatg 1200 acatagtgtt ctatggtcaa attttatagc gtacttattc caatccatca cttttatag 1260 aactaaaatt catagttcct attttaatat atatatatat attaaaaca cacattaaat 1320 gatgatttta tctcttctag gttgattgaa aattactaac taaaaaacac ggtgcctcaa 1380 acctccaacg taaatacgat ttctaagaac tgtgttttt gtaaacgcca agtgactgat 1440 taaatctctc cattctctgt ttacttctat ttggggttat ttatgctaaaa ggatattatt 1500 cattcaatag aataaatgtg agatagtcga gttatattca tagatgttac aatgaggtga 1560 ttcattcctt tgtcaaacaa tgctttctcg actcgtattt tactgtattg gatcgaaatc 1620 cttcttactc gcatggtttg ccttcgttga ttagttttgg tatgaattga tgctttgttt 1680 aagggggaaa atgaaaatgg ttcaattgga ggacaattgt ccaaatttcg ggacattatg 1740 ggttaaacac aaagaagaag tccaacagtg taattttgtt aaagattgcg ttacatttcc 1800 gaaatataaa tgagggtatt ttggggaaag gaaatcaata taggccttgg ccgggtgaga 1860 tgcgaaaaag tctcaaaact gagtgagaag cgtttgagct gggctcgcag ctattgaaaa 1920 agagagaaca aaccctttcg tcgctcttat tttcttcctt tgatctgaaa tttcctgttc 1980 cgatctcgct ttaggacgca 2000 <210> 79 <211> 2000 <212> DNA <213> Muskmelon <400> 79 aattcattcg ggattgttat gaggtaataa aaaatatctg agtgcgaaca tgataattgg 60 taaagtgaaa aaatgttcag ctattctgtt ctagatacag ggatggaagt gggaacaatg 120 ccaccttgct tattgacaat aaaatgagga gtggcaatat tttgtttctg aataaatatt 180 cactagcata acatattagt gatgattcaa actaaagtgc actaggtcac tagtttcttg 240 attcatcgtg tttggtagta atggtaggta ttgtatctta tagtattgga caaagcttta 300 ccgaccataa attgtggata atgtgcagag aagaattggc agttgaacgt tcctggatat 360 tcaagtgatg agtggaagaa tcacaacaaa aatgtaagaa aattatatta ccctctctaa 420 aacatcattc tattctctc cctaaaaaat cattctgttt caatttaact ttcaaaattt 480 tgttttagtt taaccatatt gagttttttt tcttttttaa ttatcgtagt tatcatcaag 540 tgatgtccac aagaaacgtt tggacatggt aagttggact tatctcttca agtgtttgct 600 ccatttcttc tttatcatt tgtctcaaat tttctcttct ggggtttcat cagatacgcc 660 tattgaagga agcctcctgt gtcgaaacaa atgtaaacag ccctaaagag atggtacgac 720 aaggggttgg aatgtcaatt ggtcccaaca ctctaacaag gccttcaccg agttcagaac 780 aactattatc acaaacgtct ggttcacagt tgctgcagca aatgatgagg ttttagtgta 840 ttaactacgt ttgaaactaa tgcttggtag agatcccaac tacttggtga ataaccaacc 900 ccagtgtcag ttcagggata caacaaataa aatgagattt agaggatgcc atatcagagg 960 gaacctggac tggacatctg tgtggagtgg agtgtgatga ttttagtga tacgtctttc 1020 ggaatcaatt ttttaggct gtataatatg aagttgcatt atctggaaca cgggcgtaat 1080 gttaattgta caaaatattt ggcaggtcat attagtatag gccttaagta ttgttgttgt 1140 ctaccatgaa ggacatttc caatttatga ttgataatct ttacttacaa tctcgagtca 1200 tatgaagttt gttgatcagg atcatagcac aattattaca aaaatgaaat agaagatatg 1260 attttcacc ccccccccac cccccccccc cccccccct ccattcccat cccccctttt 1320 aaactgttac attacaactt gttaactgtt gattttccag atgagagaaa gggcctactt 1380 gtcttgtaca gaaaattcat ccatgacgat aaatgcagat gacctgaacc aaacgtgaca 1440 gtaggggttt cttctatgcc acaaagctcc aagccattca tggtgcgcat gtggtacaga 1500 gaggcttgat ggagcctctt caccttggtc cttagctatc taaaaattg cttcttatgc 1560 tgatatatct cttcccatgt gcatttggtc cactccactt tcttcgtcga atatccttgg 1620 gttaatcctg aatggtaagc acaacattct tgctaattaa tccctctttt tatcctactt 1680 gccaactgta caagatgagc agaagaagaa ttgcccaatc atgaggtcat taactgcaaa 1740 aaagagaatt tatttctttc tttgagaatc tgatcttctt gagagttcat tgacagccac 1800 atgcatcaca aaatgaaatg ctgtgtggcc ctcattcatt cattcatcaa tcttcctatc 1860 ctgccatttg agtgaatgtt actccaactt gcaggaagct aaattagtac ttttttatat 1920 aaaccctatg aaactcatca agaaaccaca ccatcccaaa aaggaaacga gtgaacaact 1980 agacaactca ccccgaaaaa 2000 <210> 80 <211> 2000 <212> DNA <213> Muskmelon <400> 80 cagcgatctt cgtagaaact aattcaatgc tagctcatta tttgactttt ctcaggcaag 60 gctccgcgag gaagagaaaa ggtccaattt caggaaccaa gggcatggac aggttccggt 120 cagaagaagc tttttacgta aaccctttgc cagattgttt atgtcaagga gaattaccaa 180 atggaagtac gacttccatt ttcagatagt tcagtagaac atcaaagata aaatgctctt 240 agagagctca atgtatatgc aggcgaccac tcaacgtgtc accagctttg tacatccaat 300 aagccagcta cgatggaacg acggagtgtt tataacctga gttttggtag ttggcggagg 360 cggtgatggt ggtatagaag gaaggtcgag ggatggcaaa ccctttacgc caagtagtgg 420 aagggagtag ttggagatga acacattttg agaagtttcc aagatcactc catttggggg 480 agaggggatg ttggttattt agcacaattg ttttcatgtt ttagtaattt tatccaataa 540 tgagcgaggc attgaagcaa ttaaatttat ttttaatgat tttttcaccc ttccataggc 600 tttttctttt ttcttttcct tttagtttgc aaactttagc tccttttatc ggctgtcgaa 660 ctcatttttg aagttattga atgaaacaca gtttgggctg tgtcagatgg gtggtgaaat 720 tttatacatt ataattacta cataaaatga aatcatattg taattttcta tctatgccac 780 aatttttttt tattgcatca tgaggattaa attgtacgag tccaaatttg tacagtcatg 840 tttttaaagc tttcgagcat tgttactaat gcatggaaag gatcgattat caagtatcct 900 cccaacttca tgaaagttat tattgtctt ctaaatttgt tttagaaaat gtttaattaa 960 ttatttgaga agaaagttta actaaatcct attggttcc tctaggttg tcatacttat 1020 ccaataaca ttacgtttaa aatcaaatt attctaatgg tataagacta atgttttaaa 1080 agcataaaat tgatgaggaa ggattggaag taatactatt tattttgaag gtaacattc 1140 ttgaatgtct gtcctaaaat cactaatgtt ttcttagttt gagactttga gtcgttgaac 1200 ctctccatct tttaaaata taatacgagt ccttcacaat aacttaaaat atatactaaa 1260 tcctaattaa tgaaaataa aaaaaa ggtacaaaat cattaagcc taaaaatcta 1320 ttactccttt aaacttttc aagggtccct acaaccaatg agaactacc acgtcatttt 1380 cacaatccgt tcagtgttta gaaaagtca atcgcaccgt ccatttatcc actcgtacca 1440 agtacggtag gatctatct accgtccgat taagcacaaa gagcacagt aaatgtcaat 1500 cgtgtccatc cgccgccata ccgcacatcc ttcgtccgac cggaaggccc tatataagt 1560 cctttggttt tccgaatttc tacttcattc gctttgaa gatttcccaa tctctcgtc 1620...

Claims

1. A DNA molecule exhibiting gene regulatory activity, comprising a polynucleotide sequence consisting of SEQ ID NO: 211, wherein the polynucleotide sequence is operatively linked to a heterologous transcribed polynucleotide molecule.

2. The DNA molecule of claim 1, wherein the heterologous transcribed polynucleotide molecule comprises a gene for agricultural purposes.

3. The DNA molecule of claim 2, wherein the agronomically intended gene confers herbicide tolerance in plants.

4. The DNA molecule of claim 2, wherein the agronomically intended gene confers pest resistance in the plant.

5. A method for producing a commodity, comprising: a) Obtaining a transgenic plant or a portion thereof comprising the DNA molecule of claim 1; and b) The goods are thus produced.

6. The method of claim 5, wherein the product is selected from the group consisting of: protein concentrates, protein isolates, grains, starches, seeds, crude flour, wheat flour, biomass, and seed oils.

7. A method for expressing a transcribed polynucleotide molecule, comprising: a) Obtaining a transgenic plant comprising the DNA molecule of claim 1; and b) Cultivate the transgenic plant in which the transcribed polynucleotide is expressed.

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