A method for preparing mouse intestinal tissue wax block for slicing
By using the sandwich structure and anti-pressure layer of multi-layer non-woven fabric and filter paper layer in the preparation of mouse intestinal tissue, the problem of separation of muscle layer and mucosal layer in the prior art midgut section is solved, the morphological integrity of intestinal tissue and the clear display of submucosal morphology is achieved, and the research on intestinal diseases is promoted.
Patent Information
- Application Number
- CN202210963097.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2022-08-11
- Publication Date
- 2025-04-11
- Estimated Expiration
- 2042-08-11
AI Technical Summary
In the existing intestinal slicing technology of mouse intestine, the muscle layer and the mucosal layer are easily separated, making it difficult to observe the pathological changes in the submucosal layer, affecting the research on intestinal diseases.
A method for preparing wax blocks for mouse intestinal tissue for slices is adopted, including the detection, fixation, dehydration and embedding of intestinal tissue. Through the use of the sandwich structure of multi-layer non-woven fabric and filter paper layer and the anti-pressure layer, the morphological integrity of intestinal tissue during dehydration and waxization is ensured.
It effectively reduces the incidence of separation between the muscle layer and the mucosal layer, maintains the morphological integrity of intestinal tissue, significantly improves the morphological display of the submucosal layer, and facilitates scientific researchers to conduct comprehensive observation of intestinal diseases.