A kit for extracting RNA and use thereof

By using DNase enzyme and adding an oil phase and emulsifier in the magnetic bead RNA extraction method, the problem of DNA contamination has been solved, achieving high-purity RNA extraction and instrument automation, which is suitable for clinical testing and scientific research.

CN115537418BActive Publication Date: 2026-03-24HUNAN YEARTH BIOTECHNOLOGICAL CO LTD
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Patent Information

Application Number
CN202211388792.5
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2022-11-08
Publication Date
2026-03-24
Estimated Expiration
2042-11-08

AI Technical Summary

Technical Problem

Existing RNA extraction methods suffer from DNA contamination, which affects downstream experimental results and is not suitable for automated instrument operation. In particular, DNA contamination is severe when extracting RNA using magnetic beads, and the existing enzyme digestion environment is not suitable for the automation of magnetic bead extraction.

Method used

DNA digestion was performed during RNA extraction using the magnetic bead method with Dnase enzyme. An environment suitable for the Dnase enzyme reaction was created by adding an oil phase and an emulsifier to the Dnase enzyme reaction solution, and the instrument was automated by increasing the reaction volume.

Benefits of technology

It effectively removes DNA contamination, improves RNA purity and yield, is suitable for automated instrument operation, meets the rapid and convenient needs of clinical testing, and is applicable to RT-PCR and RNA-seq applications.

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Abstract

The application provides a kit for extracting RNA and application thereof. In the application, deoxyribonuclease (DNase) is used for DNA digestion in the process of magnetic bead extraction. The reaction environment can meet the optimal reaction environment requirement of the deoxyribonuclease (DNase), and at the same time, a hydrophobic environment is provided when the magnetic beads are adsorbed, so as to ensure the yield of nucleic acid. In addition, an emulsion of an oil is added to increase the total volume during the extraction operation, so as to facilitate the convenience of automatic extraction operation of the instrument. The extracted RNA has high yield and high purity, and the method can easily realize automatic extraction of the instrument. The requirement of extracting high-purity RNA quickly and conveniently in the process of clinical practical application is solved, the difficulty and cost of extracting high-purity RNA are greatly reduced, and the extracted RNA can be well adapted to subsequent RT-PCR and RNA-seq application.
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