An anti-aging Chinese medicine composition and its preparation method and application

Abstract

The invention discloses an anti-aging Chinese medicine composition, which is composed of the following components by weight: 10-50 parts of snow lotus extract, 10-50 parts of motherwort extract, and 10-50 parts of tribulus terrestris extract. The anti-aging Chinese medicine composition of the invention is added to cosmetics as an anti-aging ingredient to prepare an anti-aging cosmetic stock solution. The anti-aging Chinese medicine composition of the invention is a pure natural Chinese medicine extract, and various components are mutually harmonized to exert a synergistic effect, and has excellent anti-aging effects, which are mainly manifested in anti-oxidation, mite inhibition, antibacterial and anti-photoaging. In addition, the Chinese medicine composition of the invention has low irritation to the skin, is safe and reliable, is a better cosmetic functional ingredient, and can be used to prepare anti-aging cosmetics.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and specifically relates to an anti-aging Chinese medicine composition and a preparation method and application thereof. Background Art

[0002] Aging is also called aging. As the largest organ in the human body, the aging of the skin is the most direct and specific manifestation of human aging. Skin aging is the result of the combined effects of endogenous and exogenous factors, mainly including natural aging and photoaging. Skin aging is manifested by sagging, dullness, reduced elasticity, increased wrinkles, pigmentation, etc.

[0003] There are many types of anti-aging raw materials on the market, most of which are synthetic raw materials, which are easy to irritate the skin, may cause irreversible damage to the skin, and have limited effects. At present, most of the anti-aging cosmetics circulating on the market are peptide ingredients with single functions, which are not effective and expensive.

[0004] In recent years, natural plant organic cosmetics have become one of the most important trends in cosmetics R&D innovation. The Chinese medicine cosmetics market is developing rapidly and has huge development potential. Compared with chemical synthetic cosmetics, the ingredients of Chinese medicine cosmetics are derived from nature, which is more reliable and safe, and has obvious functionality and strong pertinence. Therefore, the development of a Chinese medicine composition for anti-aging cosmetics has a huge market prospect.

[0005] Chinese patent CN107998033 discloses a composition with anti-aging function, which is composed of four Chinese medicinal materials, namely, snow lotus, angelica, emblica fruit, and Cynomorium songaricum.

[0006] Chinese patent CN115645334 discloses a moisturizing anti-aging cosmetic composed of cork oak bark extract. The above patents all disclose that different Chinese medicine compositions are applied to cosmetics to achieve different anti-aging effects.

[0007] These Chinese herbal compositions are used in anti-aging cosmetics, mainly to resist the body's oxidation, but there are many factors that lead to aging. It is of great significance to develop an anti-aging cosmetic that can not only resist the body's oxidation, but also achieve the anti-aging purpose by preventing photoaging and other aspects. Summary of the invention

[0008] Purpose of the invention: The purpose of the present invention is to provide an anti-aging Chinese medicine composition and its preparation method and application. The present invention applies the anti-aging Chinese medicine composition to the field of preparing anti-aging cosmetics. The anti-aging Chinese medicine composition of the present invention is rich in a variety of functional ingredients, and achieves the purpose of anti-aging from the aspects of scavenging free radicals, inhibiting oxidation reactions, inhibiting bacteria, inhibiting mites, and preventing photoaging.

[0009] Technical solution: The purpose of the present invention is achieved through the following technical solution:

[0010] The invention provides an anti-aging traditional Chinese medicine composition, which consists of the following components by weight: 10-50 parts of snow lotus extract, 10-50 parts of motherwort extract and 10-50 parts of tribulus terrestris extract.

[0011] The present invention also provides a method for preparing the anti-aging Chinese medicine composition, comprising the following steps:

[0012] (1) Grinding the dried snow lotus into powder, passing through a 100-mesh sieve, adding 80% ethanol in an amount of 10-20 times the weight of the snow lotus powder, reflux extraction for 1-1.5 hours, adding 10-20 times the weight of the concentrate in water after concentration, boiling for 1-2 hours, concentrating, adding water, ultrasonic decolorization with activated carbon, evaporation concentration, passing the concentrate through a macroporous resin, distilling, and drying to obtain a snow lotus extract;

[0013] (2) Grind the motherwort into powder, pass through a 100-mesh sieve, add 95% ethanol in an amount of 5 to 10 times the weight of the motherwort powder, perform ultrasonic treatment for 40 to 50 minutes, remove the ethanol by distillation, add water, perform ultrasonic decolorization with activated carbon, allow to stand overnight, filter, add dichloromethane for extraction, collect the lower dichloromethane layer, distill, and dry to obtain a motherwort extract;

[0014] (3) Grind the Tribulus terrestris into powder, pass through a 100-mesh sieve, add 75% ethanol in an amount of 8-10 times the weight of the Tribulus terrestris powder, reflux extract for 1.5-2 hours, distill to remove the ethanol, pass the concentrate through a macroporous resin, distill, and dry to obtain a Tribulus terrestris extract;

[0015] (4) The snow lotus extract, the motherwort extract, and the tribulus terrestris extract are mixed in proportion to obtain the anti-aging Chinese medicine composition.

[0016] A specific method for preparing an anti-aging Chinese medicine composition of the present invention comprises the following steps:

[0017] (1) Grind the dried snow lotus into powder, pass through a 100-mesh sieve, add 80% ethanol 10 times the weight of the snow lotus powder, reflux extract at 80° C. for 1 hour, repeat twice, add 10 times the weight of the concentrate in water after rotary evaporation and boil for 1 hour, rotary evaporation and concentration, add 20 times the weight of the concentrate in water, use activated carbon ultrasonic decolorization for 45 minutes, evaporate and concentrate, pass the concentrate through AB-8 macroporous resin, rotary evaporation, and dry to obtain the snow lotus extract;

[0018] (2) Grind the motherwort into powder, pass through a 100-mesh sieve, add 95% ethanol in an amount of 5 times the weight of the motherwort powder, ultrasonicate at 360 W for 40 min, repeat twice, remove the ethanol by rotary evaporation, add 20 times the weight of water of the concentrate, use activated carbon for ultrasonic decolorization for 45 min, let stand overnight, filter, add 20 mL of dichloromethane for extraction 3 times, collect the lower dichloromethane layer, rotary evaporate, and dry to obtain the motherwort extract;

[0019] (3) Grind the Tribulus terrestris into powder, pass through a 100-mesh sieve, add 75% ethanol in an amount of 8 times the weight of the Tribulus terrestris powder, reflux extract at 75° C. for 1.5 h, repeat twice, remove the ethanol by rotary evaporation, pass the concentrate through AB-8 macroporous resin, rotary evaporation, and dry to obtain the Tribulus terrestris extract;

[0020] (4) The snow lotus extract, the motherwort extract, and the tribulus terrestris extract are mixed in proportion to obtain the anti-aging Chinese medicine composition.

[0021] The present invention also provides application of the anti-aging Chinese medicine composition in preparing anti-aging cosmetics.

[0022] A preferred embodiment of the present invention is to add the anti-aging Chinese medicine composition as an anti-aging ingredient into cosmetics to prepare an anti-aging cosmetic stock solution.

[0023] The present invention also provides an anti-aging cosmetic stock solution, which is composed of the anti-aging Chinese medicine composition and cosmetic auxiliary materials.

[0024] Preferably, the amount of the anti-aging Chinese medicine composition added is 0.01-0.1% of the mass of the cosmetic stock solution. If the amount is too little, it is difficult to ensure the efficacy of the cosmetic stock solution; if the amount is too much, it is difficult to ensure the safety of the cosmetic stock solution, and the stability of the cosmetic stock solution will be reduced.

[0025] A specific implementation of the present invention is: the anti-aging cosmetic stock solution, calculated by mass percentage, includes: 0.01-0.1% of the anti-aging Chinese medicine composition, 0.5% of xanthan gum, 0.2% of sodium hyaluronate, 1.0% of phenoxyethanol, and the balance is water.

[0026] Preferably, the cosmetic auxiliary material is at least one of a thickener, a humectant, a stabilizer, a suspending agent, a moisturizer or a solvent.

[0027] Preferably, the dosage form of the cosmetic stock solution is a solution.

[0028] Beneficial effects:

[0029] The anti-aging Chinese medicine composition of the present invention is all pure natural Chinese medicine extracts, and the synergistic effect between the effective ingredients is utilized to exert the drug effect. Studies have found that the Chinese medicine composition of the present invention has excellent anti-aging effects, mainly in terms of anti-oxidation, anti-mite, anti-bacterial and anti-photoaging. The Chinese medicine composition of the present invention has little irritation to the skin, is safe and reliable, is a good cosmetic functional ingredient, and can be used to prepare anti-aging cosmetics. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 is the cell clearance rate of each test sample;

[0031] Figure 2 is the survival rate of cells damaged by UVB for each test sample;

[0032] Figure 3 is the enzyme inhibition effect of each test sample;

[0033] Figure 4 is the inhibitory effect of each test sample on Staphylococcus aureus;

[0034] Figure 5 It is the inhibitory effect of each test sample on Escherichia coli. DETAILED DESCRIPTION

[0035] The technical solution of the present invention is described in detail below through specific embodiments, but the protection scope of the present invention is not limited to the embodiments.

[0036] The raw materials used in the present invention are all common commercially available products.

[0037] In the embodiments of the present invention, deionized water is used when preparing sample solutions of different concentrations.

[0038] Example 1 An anti-aging Chinese medicine composition

[0039] (1) Grind the dried snow lotus into powder, pass through a 100-mesh sieve, add 80% ethanol in an amount 10 times the weight of the snow lotus powder, reflux extract at 80° C. for 1 hour, repeat twice, concentrate by rotary evaporation, add water 10 times the weight of the concentrate and boil for 1 hour, concentrate by rotary evaporation, add water 20 times the weight of the concentrate, use activated carbon ultrasonic decolorization for 45 minutes, evaporate and concentrate, pass the concentrate through AB-8 macroporous resin, use 70% ethanol for elution, rotary evaporation, and dry at 50° C. for 48 hours to obtain the snow lotus extract.

[0040] (2) Grind the motherwort into powder, pass through a 100-mesh sieve, add 95% ethanol in an amount of 5 times the weight of the motherwort powder, ultrasonicate at 360 W for 40 min, repeat twice, remove the ethanol by rotary evaporation, add 20 times the weight of water of the concentrate, use activated carbon for ultrasonic decolorization for 45 min, let stand overnight, filter, add 20 mL of dichloromethane for extraction 3 times, collect the lower dichloromethane layer, rotary evaporate, and dry at 50° C. for 48 h to obtain the motherwort extract.

[0041] (3) Grind the Tribulus terrestris into powder, pass through a 100-mesh sieve, add 75% ethanol in an amount of 8 times the weight of the Tribulus terrestris powder, reflux extract at 75°C for 1.5 hours, repeat twice, remove the ethanol by rotary evaporation, pass the concentrate through AB-8 macroporous resin, elute with 70% ethanol, rotary evaporate, and dry at 50°C for 48 hours to obtain the Tribulus terrestris extract.

[0042] (4) Mix 17 mg of snow lotus extract, 17 mg of Leonurus japonicus extract, and 17 mg of Tribulus terrestris extract to obtain an anti-aging Chinese medicine composition.

[0043] Example 2 An anti-aging Chinese medicine composition

[0044] According to the extraction and purification method described in Example 1, snow lotus extract, motherwort extract and tribulus terrestris extract were obtained.

[0045] Take 10 mg of snow lotus extract, 10 mg of motherwort extract, and 10 mg of tribulus terrestris extract to obtain an anti-aging Chinese medicine composition.

[0046] Example 3 An anti-aging Chinese medicine composition

[0047] According to the extraction and purification method described in Example 1, snow lotus extract, motherwort extract and tribulus terrestris extract were obtained.

[0048] Take 50 mg of snow lotus extract, 50 mg of motherwort extract, and 50 mg of tribulus terrestris extract to obtain an anti-aging Chinese medicine composition.

[0049] Comparative Example 1

[0050] The dried snow lotus was ground into powder, passed through a 100-mesh sieve, 80% ethanol in an amount 10 times the weight of the snow lotus powder was added, reflux extraction was performed at 80°C for 1 hour, repeated twice, concentrated by rotary evaporation, water 10 times the weight of the concentrate was added, and decocted for 1 hour, concentrated by rotary evaporation, water 20 times the weight of the concentrate was added, activated carbon was used for ultrasonic decolorization for 45 minutes, evaporated and concentrated, the concentrate was passed through AB-8 macroporous resin, eluted with 70% ethanol, rotary evaporated, and dried at 50°C for 48 hours to obtain the snow lotus extract.

[0051] Comparative Example 2

[0052] Grind the motherwort into powder, pass through a 100-mesh sieve, add 95% ethanol in an amount of 5 times the weight of the motherwort powder, ultrasonicate at 360 W for 40 minutes, repeat twice, remove the ethanol by rotary evaporation, add 20 times the weight of water of the concentrate, use activated carbon for ultrasonic decolorization for 45 minutes, let stand overnight, filter, add 20 mL of dichloromethane to extract 3 times, collect the lower dichloromethane layer, rotary evaporate, and dry at 50°C for 48 hours to obtain the motherwort extract.

[0053] Comparative Example 3

[0054] The Tribulus terrestris was ground into powder, passed through a 100-mesh sieve, 75% ethanol in an amount of 8 times the weight of the Tribulus terrestris powder was added, and reflux extraction was performed at 75°C for 1.5 hours, and the extraction was repeated twice. The ethanol was removed by rotary evaporation, and the concentrated solution was passed through AB-8 macroporous resin, eluted with 70% ethanol, rotary evaporated, and dried at 50°C for 48 hours to obtain the Tribulus terrestris extract.

[0055] Example 4 Preparation of anti-aging cosmetic stock solution

[0056] Anti-aging cosmetic concentrate, expressed in percentage by mass, comprising:

[0057]

[0058] Component A is fully stirred and dissolved at 40°C and mixed evenly, and component B is added to component A and homogenized to obtain the cosmetic stock solution.

[0059] Example 5 Preparation of anti-aging cosmetic stock solution

[0060] Anti-aging cosmetic concentrate, expressed in percentage by mass, comprising:

[0061]

[0062]

[0063] Component A is fully stirred and dissolved at 40°C and mixed evenly, and component B is added to component A and homogenized to obtain the cosmetic stock solution.

[0064] Example 6 Preparation of anti-aging cosmetic stock solution

[0065] Anti-aging cosmetic concentrate, expressed in percentage by mass, comprising:

[0066]

[0067] Component A is fully stirred and dissolved at 40°C and mixed evenly, and component B is added to component A and homogenized to obtain the cosmetic stock solution.

[0068] Human skin is generally weakly acidic, with a pH value ranging from about 4.5 to 6.5. The pH value of the stock solution prepared in Examples 4-6 was measured using a pH meter, and the pH value range was 6.7-7.1. According to the quality index of liquid cosmetics, the pH value of the product should be in the range of 4.0-8.5, so the pH value of the prepared cosmetic stock solution meets the requirements.

[0069] According to the content of the cosmetic stability test method, the cosmetic stock solutions prepared in Examples 4-6 were subjected to centrifugal acceleration tests and heat and cold resistance tests. The samples did not precipitate or change color at different temperatures or after returning to room temperature, indicating that the stock solutions had good stability.

[0070] Example 7 Evaluation of free radical scavenging effect

[0071] The DPPH method has become one of the common in vitro chemical methods for determining antioxidant properties, mainly because of its advantages such as high sensitivity, simple technology and good reproducibility.

[0072] The principle is as follows: The assay method uses a spectrophotometer to measure the absorbance of DPPH (1,1-diphenyl-2-trinitrophenylhydrazine) alcohol solution at 526nm. After the DPPH free radical is reduced by the antioxidant, the color of the solution will gradually lighten to light yellow. Its ability is expressed by the clearance rate. The greater the clearance rate, the stronger the anti-aging ability.

[0073] The calculation formula of clearance rate: Clearance rate % = (A0-A1) / A0×100%

[0074] In the formula,

[0075] A0 is the absorbance measured by adding 2 mL of anhydrous ethanol to 2 mL of DPPH ethanol solution.

[0076] A1 is the absorbance measured by adding 2 mL of sample solution to 2 mL of DPPH ethanol solution.

[0077] 1. Determination of the maximum detection wavelength of DPPH

[0078] The concentration was 2×10 -4 mol·L -1 The DPPH ethanol solution was placed in a UV spectrophotometer and scanned at the full wavelength to determine that the maximum absorption wavelength of DPPH was 526 nm.

[0079] 2. Antioxidant capacity tests were performed on the positive control (water-soluble vitamin C), Example 1, Comparative Example 1, Comparative Example 2 and Comparative Example 3. The above test samples were diluted to different concentrations (0.01 mg / mL, 0.02 mg / mL, 0.03 mg / mL, 0.04 mg / mL, 0.05 mg / mL, 0.06 mg / mL), and each sample was measured three times in parallel. The average clearance rate was taken. The results are as follows: Figure 1 shown.

[0080] Depend on Figure 1 It can be seen that under the concentration condition of 0.01-0.06 mg / mL, the scavenging effect of the embodiments and comparative examples on DPPH is concentration-dependent, and the higher the concentration, the stronger the scavenging effect. Comparative Example 1 has the strongest scavenging effect on DPPH free radicals, followed by the composition of Example 1. The composition of Example 1 has excellent antioxidant capacity, and the antioxidant capacity is stronger than that of Comparative Examples 2 and 3, and slightly weaker than that of Comparative Example 1.

[0081] Example 8 Safety Evaluation

[0082] HaCaT cells (human immortalized keratinocytes) were selected, which is a spontaneously immortalized human keratinocyte cell line. Toxicity experiments were carried out using HaCaT cells, and cell survival rates were determined using the MTT method to evaluate the cytotoxic effects of the examples and comparative examples.

[0083] Experimental method: HaCaT cells (Pusano Biotech, Wuhan) were taken and the cell concentration was adjusted to 1×10 cells by adding Dulbecco's modified Eagle's medium (DMEM) containing 10% heat-inactivated fetal bovine serum (FBS, Thermo Fisher Scientific, USA), 100 U / mL streptomycin and 100 U / mL penicillin. 5 / mL, inoculated in a 96-well plate, 100μL per well, cultured in an incubator at 37°C, 5% CO2 and 95% humidity for 24h, and discarded the culture supernatant after it adheres to the wall. Rinse with phosphate buffer solution (PBS, 0.1M, pH=7.4, Thermo Fisher Scientific, USA) 2-3 times, add 100μL of the above DMEM culture medium containing samples of different concentrations (0.1-1.0mg / mL), set 5 replicates for each group, and continue to culture in an incubator at 37°C, 5% CO2 and 95% humidity for 24h before testing.

[0084] 20 μL of 5 mg / mL MTT (thiazolyl blue, Sane Chemical Technology) solution was added to each well and cultured in an incubator at 37°C, 5% CO2 and 95% humidity for 4 h. After discarding the supernatant, 150 μL of DMSO (dimethyl sulfoxide, Sinopharm Group) was added to each well and shaken for 15 min in the dark. The absorbance of each well was measured at a wavelength of 490 nm using an enzyme reader, and the cell survival rate was calculated.

[0085] The calculation formula of cell survival rate: survival rate % = (A-A0) / (A1-A0)×100%

[0086] Where: A is the absorbance of the sample well, A0 is the absorbance of the negative control well, and A1 is the absorbance of the positive control well.

[0087] The negative control wells were DMEM medium only, and the positive control wells were DMEM medium containing HaCaT cells.

[0088] Cytotoxicity tests were performed on Example 1, Comparative Example 1, Comparative Example 2 and Comparative Example 3 at different concentrations, and the results are shown in Table 2.

[0089] Table 2 Cell survival rate at different concentrations (%)

[0090]

[0091] It can be seen from the test results in Table 2 that the Chinese herbal extracts of Example 1, Comparative Example 1, Comparative Example 2 and Comparative Example 3 have no obvious cytotoxic effect when the concentration is ≤1.0 mg / mL, that is, they can be used as cosmetic raw materials.

[0092] Example 9 Evaluation of Cell Anti-Photoaging Activity

[0093] The UVB (medium wave ultraviolet) dose was screened according to the method of Example 8. Before UVB irradiation, the steps were the same as those of Example 9. After PBS washing, 100 μL PBS was added to each well. The dose was 6-24 mJ / cm 2 After UVB irradiation, PBS was aspirated and replaced with 100 μL DMEM medium. Five replicate wells were set up in each group and cultured in an incubator at 37°C, 5% CO2 and 95% humidity for 24 hours before testing. As the UVB dose increased, cell viability gradually decreased. When the UVB dose reached 18 mJ / cm 2 When the cell survival rate drops to about 50%, the cells are obviously damaged, but there is still a possibility of repair, so the dose of 18mJ / cm 2 UVB was used to construct a photoaging damage model.

[0094] The protective effects of Example 1, Comparative Example 1, Comparative Example 2 and Comparative Example 3 on UVB-induced photoaging damage of HaCaT cells were investigated. The steps before UVB irradiation were the same as those in Example 9. The cells were rinsed several times with PBS, and 100 μL of culture medium containing samples of different concentrations (0.1-0.8 mg / mL) was added respectively. The cells were cultured in an incubator at 37°C, 5% CO2 and 95% humidity for 24 h. After rinsing with PBS, 100 μL of PBS was added to each well. The optimal dose of 18 mJ / cm 2 After light damage treatment, the cells were replaced with fresh DMEM medium. Five replicate wells were set up in each group and cultured in an incubator at 37°C, 5% CO2 and 95% humidity for 24 hours before testing. Figure 2 As shown, Figure 2 NC refers to the survival rate of Hacat cells damaged only by UVB.

[0095] from Figure 2 It can be seen that within the concentration range of 0.1-0.8 mg / mL, the increasing effect of Comparative Example 3 on the survival rate of Hacat cells damaged by UVB is dose-dependent, and the increasing effect is the best, while the increasing effect of Example 1 on the survival rate of Hacat cells damaged by UVB is significantly better than that of Comparative Example 1 and Comparative Example 2. Therefore, Example 1 has excellent anti-photoaging ability, and the anti-photoaging ability is stronger than that of Comparative Example 1 and Comparative Example 2, and slightly weaker than that of Comparative Example 3.

[0096] Example 10 Evaluation of mite inhibition effect

[0097] There are two types of mites (Demodex) that parasitize the human body: folliculomorphic mites and lipid mites. The mites are slender and worm-like, with a body length of 0.1 to 0.2 mm. There are palates and points in the front of the body, and the whole limbs are short needle-shaped. More research data show that the infection rate of human Demodex in my country is relatively high, reportedly reaching more than 98%. Because the human body has no immunity to the infection of mites, people of different ages, different nationalities, and different genders can be infected. After being infected with mites, it will cause the following skin problems: dandruff, itching, spots, oily face, bacterial growth, rough skin, blackheads, acne, acne, etc., which will cause skin aging.

[0098] At the biochemical level, chitinase inhibitors can specifically bind to chitinase, inhibit the activity of chitinase, and prevent insect larvae and pupae from molting, thus playing an insecticidal role.

[0099] The principle is as follows: After chitinase degrades chitin, it generates reducing ends of monomers or oligomers, which can react with a specific 3,5-dinitrosalicylic acid colorimetric agent to develop a color reaction. The activity of chitinase is calculated by measuring the change in absorbance. If there is a chitinase inhibitory compound, the absorbance value is significantly lower than the control.

[0100] The chitinase inhibition rate of each test sample (Example 1, Comparative Example 1, Comparative Example 2 and Comparative Example 3) at 0.5 mg / mL was detected. Chitinase, as a specific hydrolase of chitin, can hydrolyze chitin to generate N-acetylglucosamine. Therefore, the commonly used method for determining the chitinase inhibition rate is the DNS (3,5-dinitrosalicylic acid) colorimetric method. Add 1mL of 0.5% chitin colloid solution from Shanghai Aladdin Biochemical Technology Co., Ltd., 0.5mL of 0.5U / mL chitinase solution from Shanghai Yuanye Biotechnology Co., Ltd., 0.5mL of the test solution and the reference solution into the test tube, heat the test tube containing the reaction solution in a 37℃ water bath for 1.5h, mix evenly, take 0.5mL of the reaction solution and 1.5mL of DNS reagent from Shanghai Yuanye Biotechnology Co., Ltd. and mix evenly, boil for 10min to make a color reaction, cool to room temperature in an ice water bath, add distilled water to 3.5mL, measure the absorbance value at 500nm in a UV spectrophotometer, and repeat three times for each group. Calculate the enzyme activity inhibition rate of each test object according to the formula.

[0101] Inhibition rate % = (A-△A) / A×100%

[0102] Where A is the absorbance of the control group, and △A is the difference between the absorbance of the test group and the blank test group.

[0103] The control group consisted of 1 mL of 0.5% chitin colloid solution, 0.5 mL of 0.5 U / mL chitinase solution and 0.5 mL of phosphate buffer solution. After reacting in a 37° C. water bath for 1.5 h, the absorbance value was measured using an ultraviolet spectrophotometer.

[0104] The blank test group consisted of 1 mL of 0.5% chitin colloid solution, 0.5 mL of phosphate buffer solution and 0.5 mL of the test solution. After reacting in a 37° C. water bath for 1.5 h, the absorbance value was measured using an ultraviolet spectrophotometer.

[0105] The test group consisted of 1 mL of 0.5% chitin colloid solution, 0.5 mL of 0.5 U / mL chitinase solution and 0.5 mL of test solution. After reacting in a 37° C. water bath for 1.5 h, the absorbance value was measured using an ultraviolet spectrophotometer.

[0106] Three groups were tested in parallel and the average value was taken. The results are as follows Figure 3 As shown in Figure 2, the higher the enzyme inhibition rate, the stronger the mite inhibition ability.

[0107] It can be seen from the figure that the Chinese medicine composition of Example 1 has excellent mite inhibition ability, and the mite inhibition ability is stronger than that of Comparative Example 1 and Comparative Example 3, and slightly weaker than that of Comparative Example 2.

[0108] Example 11 Evaluation of antibacterial effect

[0109] Clinically, Staphylococcus aureus and Escherichia coli can cause folliculitis, and red papules will appear on the patient's skin surface, and gradually develop into pustules, surrounded by a red halo, which leads to skin aging. The plate count method was used to detect the antibacterial ability of each test sample. 0.5g beef extract, 1.0g peptone, 0.5g sodium chloride and 1.5g agar from Sinopharm Reagent Group Co., Ltd. were dissolved in 100mL distilled water, heated and stirred, and sterilized at 121℃ for 15min, cooled to 45-55℃, and placed in a 55℃ water bath for insulation, and reserved for use. Use physiological saline to ultrasonically dissolve the sample to be tested to make a solution with a concentration of 0.5mg / mL, and pass the sample solution to be tested through a 0.22μm filter membrane. Put the sample solution and physiological saline into 5mL sterilized test tubes respectively, add 100μL of the prepared bacterial suspension, mix well, react for 10min, take 0.5mL of the reacted sample solution and add 5mL of physiological saline to mix well, take 200μL of the sample solution and drop it into the culture dish, pour 9mL of culture medium cooled to 45℃ into the plate, gently rotate the culture dish to mix the sample solution and culture medium evenly, wait for it to solidify, invert the plate, and culture it in a 37℃ constant temperature incubator. After 48h, count the live bacteria in the colony and calculate the antibacterial rate of each sample according to the formula.

[0110] Antibacterial rate = ((AB)) / A×100%

[0111] Where A is the average colony count of saline solution, and B is the average colony count of sample solution. The Escherichia coli and Staphylococcus aureus strains are from Beina Biotechnology.

[0112] The test sample with a concentration of 0.5 mg / mL was selected to explore its antibacterial ability. The results are as follows Figure 4 , Figure 5 As shown in Table 3, the blank control in the graph is the number of bacterial colonies cultured with the addition of physiological saline.

[0113] Table 3 Inhibitory effect of each test sample on Staphylococcus aureus and Escherichia coli

[0114]

[0115] According to the national standard, products that can kill more than 50% of bacteria are called antibacterial, and products that can kill more than 90% of bacteria are called bactericidal. As shown in the chart, the inhibition rate of Example 1 on Staphylococcus aureus is much higher than that of Comparative Example 1, and slightly lower than that of Comparative Example 2 and Comparative Example 3, and has a good antibacterial effect on Staphylococcus aureus; the inhibition rate of Example 1 on Escherichia coli is much higher than that of Comparative Example 1, and slightly higher than that of Comparative Example 2 and Comparative Example 3, and has a good bactericidal effect on Escherichia coli. Therefore, the Chinese medicine composition of Example 1 has excellent antibacterial ability against Staphylococcus aureus and Escherichia coli, and the inhibition of snow lotus, Tribulus terrestris and Leonurus japonicus on Escherichia coli plays a synergistic role, so the antibacterial effect of the Chinese medicine composition is the best.

[0116] From a comprehensive perspective, the Chinese medicine composition of Example 1 has good effects in scavenging free radicals, inhibiting oxidative reactions, inhibiting bacteria, inhibiting mites, and preventing photoaging. Compared with Comparative Example 1, Comparative Example 2 and Comparative Example 3, the effect is more comprehensive, so the Chinese medicine composition of Example 1 has better anti-aging effect.

[0117] As described above, although the present invention has been shown and described with reference to specific preferred embodiments, it should not be construed as limiting the present invention itself. Various changes in form and details may be made without departing from the spirit and scope of the present invention as defined in the appended claims.

Claims

1. An anti-aging Chinese medicine composition, characterized in that: The invention is composed of the following ingredients by weight: 10-50 parts of snow lotus extract, 10-50 parts of motherwort extract and 10-50 parts of tribulus terrestris extract; The preparation method of the anti-aging Chinese medicine composition comprises the following steps: (1) Grind the dried snow lotus into powder, pass through a 100-mesh sieve, add 80% ethanol in an amount of 10-20 times the weight of the snow lotus powder, reflux extract for 1-1.5 hours, add 10-20 times the weight of the concentrate in water after concentration, boil for 1-2 hours, concentrate, add water, ultrasonically decolorize with activated carbon, evaporate and concentrate, pass the concentrate through a macroporous resin, distill, and dry to obtain a snow lotus extract; (2) Grind the motherwort into powder, pass through a 100-mesh sieve, add 95% ethanol in an amount of 5 to 10 times the weight of the motherwort powder, perform ultrasonic treatment for 40 to 50 minutes, remove the ethanol by distillation, add water, perform ultrasonic decolorization with activated carbon, let stand overnight, filter, add dichloromethane for extraction, collect the lower dichloromethane layer, distill, and dry to obtain the motherwort extract; (3) Grind the Tribulus terrestris into powder, pass through a 100-mesh sieve, add 75% ethanol in an amount of 8-10 times the weight of the Tribulus terrestris powder, reflux extract for 1.5-2 hours, remove the ethanol by distillation, pass the concentrate through a macroporous resin, distill, and dry to obtain a Tribulus terrestris extract; (4) The snow lotus extract, the motherwort extract and the tribulus terrestris extract are mixed in proportion to obtain the anti-aging Chinese medicine composition.

2. Use of the anti-aging Chinese medicine composition according to claim 1 in the preparation of anti-aging cosmetics.

3. An anti-aging cosmetic stock solution, characterized in that: The anti-aging Chinese medicine composition according to claim 1 and cosmetic excipients are composed.

4. The anti-aging cosmetic stock solution according to claim 3, characterized in that: The amount of the anti-aging Chinese medicine composition added is 0.01-0.1% of the mass of the cosmetic stock solution.

5. The anti-aging cosmetic solution according to claim 3, characterized in that: The cosmetic auxiliary material is at least one of a thickener, a stabilizer, a humectant or a solvent.

6. The anti-aging cosmetic stock solution according to claim 3, characterized in that: The dosage form of the cosmetic stock solution is a solution.

Images