A repair composition and its application

Abstract

This invention provides a repair composition and its application. The composition includes olive leaf extract and panthenol, which can alleviate UV-induced skin damage by reducing oxidative stress, inhibiting inflammatory responses, reducing cell apoptosis, and strengthening the skin barrier structure; that is, the composition has good repairing effects on skin damage. This invention also provides the application of the above-mentioned repair composition in cosmetics.

Description

Technical Field

[0001] This invention relates to the field of cosmetics, and mainly to a repair composition and its application. Background Technology

[0002] The olive tree (Olea europaea L.) is an evergreen tree belonging to the Oleaceae family and the Olea genus. Native to the Mediterranean coast, it is a world-renowned woody oilseed tree. Olive oil, extracted from the fruit, is widely used in the food, pharmaceutical, and cosmetic industries, and possesses certain anti-cancer, anti-aging, and wound-healing properties. Olive leaves are rich in active ingredients, including oleuropein, hydroxytyrosol, flavonoids, and polyphenols. Studies have found that olive leaf extracts have antioxidant, anti-inflammatory, antibacterial, and antiviral effects, showing broad application prospects in the pharmaceutical and cosmetic fields.

[0003] D-Panthenol, also known as provitamin B5, has the chemical formula C9H. 19 NO4, with the structure (R-)2,4-dihydroxy-N-(3-hydroxypropyl)-3,3-dimethylbutyramide, is widely used in the food, pharmaceutical, and cosmetic industries. D-panthenol, due to its small molecular weight, is easily absorbed and is converted into pantothenic acid in the body, further forming coenzyme A, which participates in various biological processes. As a moisturizer, panthenol can penetrate the stratum corneum, increase skin hydration, and strengthen the skin barrier.

[0004] One of the most important triggers for extrinsic skin aging is sunlight exposure, also known as "photoaging." Ultraviolet (UV) radiation can cause DNA and mitochondrial damage through oxidative stress, leading to protein structural disruption and degradation, releasing apoptosis factors, activating apoptosis signaling pathways, and ultimately causing cell death. Reducing the stimulation of cells by UV radiation and regulating the apoptosis process can mitigate the damage caused by photoaging to the skin. In addition, external stimuli such as UV radiation and air pollution can induce skin inflammation, leading to reactions such as pain, itching, erythema, and edema. Chronic inflammation is also a major cause of skin aging. Alleviating inflammatory responses helps maintain healthy skin homeostasis and slows down the skin aging process.

[0005] Panthenol is widely used in cosmetics as a moisturizer, increasing the moisture content of the stratum corneum and maintaining a healthy skin barrier. For example, patent CN113332205A combines panthenol with active ingredients such as citrus peel extract to achieve whitening, evening out, and brightening effects on skin tone. Olive leaf extract has excellent free radical scavenging properties and highly effective antibacterial effects against various bacteria, including Propionibacterium acnes. For example, patent CN115025026A combines olive leaf extract with various natural plant extracts to achieve soothing, repairing, and anti-allergic effects.

[0006] Panthenol and olive leaf extract have different effects, but there are currently no studies on the repair effects of panthenol and their combination on ultraviolet light damage or on reducing oxidative stress. Summary of the Invention

[0007] In response to the aforementioned problems, this invention describes the synergistic effect of a combination of panthenol and olive leaf extract on the skin, which has a wider range of applications in cosmetics.

[0008] On one hand, the present invention provides the use of a composition for preparing an agent to reduce oxidative stress in the skin, the composition comprising the following components: panthenol and olive leaf extract.

[0009] Furthermore, the composition provided by the present invention includes olive leaf extract and panthenol, which have a synergistic effect on inhibiting ultraviolet-induced oxidative stress.

[0010] Specifically, the composition provided by this invention can reduce the content of reactive oxygen species in fibroblasts after UVA irradiation.

[0011] Specifically, the composition provided by this invention can reduce the content of reactive oxygen species in the epidermal model after UVB irradiation.

[0012] Furthermore, the olive leaf extract in the composition can be extracted by methods such as maceration, ultrasonic-assisted extraction, microwave-assisted extraction, and supercritical carbon dioxide extraction. The extract may be in the form of a solution or a solid.

[0013] Furthermore, the olive leaf extract contains more than 0.1% oleuropein.

[0014] Preferably, the olive leaf extract is purified by macroporous resin adsorption.

[0015] In the composition, panthenol is dextro-panthenol (D-panthenol).

[0016] Preferably, panthenol is natural dextrorotatory panthenol derived from fermentation.

[0017] Specifically, the aforementioned natural dextrorotatory panthenol from fermentation refers to the final product obtained by condensing the panthenol precursor D-pantolytic acid lactone with 3-aminopropanol.

[0018] Furthermore, in the composition, the ratio of oleuropein to panthenol in the olive leaf extract is (0.001-1):1.

[0019] Preferably, the ratio of oleuropein to panthenol in olive leaf extract is (0.01-0.1):1.

[0020] Further preferred, the ratio of oleuropein to panthenol in the olive leaf extract is 0.075:1.

[0021] Furthermore, the composition is used in cosmetics at a concentration ranging from 0.01% to 30%.

[0022] Preferably, the composition is present in cosmetics at a concentration ranging from 0.1% to 10%.

[0023] Furthermore, oxothiazolidinic acid is added to the composition to increase stability: improve the oxidative discoloration problem of olive leaf extract and slow down the degradation of the main active substance in olive leaf extract, bitter orange.

[0024] Furthermore, the ratio of oxothiazolidine carboxylic acid to oleuropein in olive leaf extract is (0.01-10):1.

[0025] On the other hand, the present invention provides the use of the above-described composition in the preparation of formulations that inhibit inflammatory responses, reduce apoptosis, or enhance the structure of the skin barrier.

[0026] Furthermore, the composition reduces inflammatory response by inhibiting the expression of IFNA7, IL15, CXCL10 and SELE genes, reduces apoptosis by promoting FASTKD3 gene expression and inhibiting CASP10 gene expression, and enhances skin barrier structure by promoting GRHL3 gene expression.

[0027] Furthermore, the composition exhibits a synergistic effect on the expression levels of the FASTKD3, CASP10, and SELE genes.

[0028] On the other hand, the present invention provides the use of the above composition in preparation for reducing skin damage caused by ultraviolet radiation.

[0029] Furthermore, the composition can significantly reduce the number of sunburned cells in a skin model after ultraviolet irradiation, exhibiting a synergistic effect compared to single ingredients.

[0030] The beneficial effects of this invention include:

[0031] 1. The composition provided by this invention comprises olive leaf extract and panthenol, which have a synergistic effect in inhibiting ultraviolet-induced oxidative stress. Specifically, the composition provided by this invention can reduce the content of reactive oxygen species in fibroblasts after UVA irradiation and reduce the content of reactive oxygen species in an epidermal model after UVB irradiation.

[0032] 2. The composition can reduce UV-induced skin damage by inhibiting inflammatory responses, reducing cell apoptosis, and enhancing the skin barrier structure. Specifically, the composition can significantly reduce the number of sunburned cells in a skin model after UV irradiation, exhibiting a synergistic effect compared to single ingredients.

[0033] 3. The composition can reduce inflammatory response by inhibiting the expression of IFNA7, IL15, CXCL10 and SELE genes, reduce cell apoptosis by promoting the expression of FASTKD3 gene and inhibiting the expression of CASP10 gene, and enhance skin barrier structure by promoting the expression of GRHL3 gene; the composition has a synergistic effect on the expression levels of FASTKD3, CASP10 and SELE genes. Attached Figure Description

[0034] Figure 1 Compared to the negative control, the ROS content in the epidermal model was significantly reduced after using panthenol, olive leaf extract, and the combination (green fluorescence intensity indicates ROS content, corresponding to the lighter-colored areas in the image).

[0035] Figure 2 Compared to the negative control, the changes in the number of sunburned cells in the epidermal model after application of panthenol, olive leaf extract, and the combination (white triangles point to sunburned cells)

[0036] Figure 3 Compared to the negative control, GO enrichment analysis of upregulated genes in the epidermal model after application of the composition

[0037] Figure 4 GO enrichment analysis of downregulated genes in the epidermal model after application of the composition compared to the negative control.

[0038] Figure 5 Effects of different antioxidants on the stability of olive leaf extract in formulations (Groups 1-10)

[0039] Figure 6 Effect of 0.15% oxothiazolidine carboxylic acid on the stability of olive leaf extract (groups 11-18)

[0040] Figure 7 Effect of 0.3% oxothiazolidine carboxylic acid on the stability of olive leaf extract (groups 19-30) Detailed Implementation

[0041] The present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments. These embodiments are for illustrative purposes only and are not intended to limit the scope of the invention. Unless otherwise specified, the experimental methods used in the following embodiments are conventional methods; the materials and reagents used, unless otherwise specified, are commercially available.

[0042] Example 1: Preparation of the Repair Composition

[0043] The panthenol used in the following examples was purchased from Anhui Huaheng Biotechnology Co., Ltd., with a purity greater than 98%.

[0044] Olive leaf extract, containing 37% oleuropein, was obtained by purifying olive leaves from Longnan, Gansu Province, using macroporous resin adsorption. The specific process was as follows: dried olive leaves were pulverized and added to a 70% ethanol aqueous solution, then heated under reflux three times. The extracts were combined and filtered to obtain a concentrated extract. The extract was then used to adsorb the active ingredients using a macroporous resin column. After adsorption, the resin column was washed with water to remove impurities, followed by desorption using a 70% ethanol aqueous solution. The desorbed solution was collected and dissolved in an appropriate amount of aqueous alcohol solution to prepare the final product, the olive leaf extract.

[0045] Oxothiazolidinic acid was added to olive leaf extract to increase stability (improving the oxidative discoloration problem of olive leaf extract and slowing down the degradation of oleuropein, the main active ingredient in olive leaf extract). This step was carried out by adding it to the formulation together with the olive leaf extract. Oxothiazolidinic acid is an amino acid derivative, often used as a whitening active ingredient in cosmetics, and has good antioxidant effects. It was purchased from Nanjing Spaco Biochemical Industry Co., Ltd.

[0046] The ratio of oxothiazolidine carboxylic acid to oleuropein in olive leaf extract is (0.01-10):1. The ratio of oleuropein to panthenol in olive leaf extract is (0.001-1):1 by weight.

[0047] The single components and compositions in the following examples were obtained through the processes and methods described above.

[0048] Example 2: Composition reduces oxidative stress in cells

[0049] Reactive oxygen species (ROS) include superoxide radicals, hydrogen peroxide, and its downstream product peroxides, which participate in physiological processes such as cell growth, proliferation, development, differentiation, aging, and apoptosis. Various environmental stimuli, such as ultraviolet radiation and air pollution, can lead to oxidative stress, an imbalance between oxidation and antioxidation, specifically manifested as increased ROS levels. This damages biomolecules such as nucleic acids and proteins, ultimately leading to skin aging and disease. DCFH-DA is a commonly used intracellular ROS detection probe: it is non-fluorescent itself, but after entering the cell, it is hydrolyzed by esterases into DCFH. DCFH can be further oxidized by ROS into DCF, a green fluorescent substance that cannot penetrate the cell membrane. The fluorescence intensity is directly proportional to the ROS level. The effects of olive leaf extract, panthenol, and their combination on ROS levels in fibroblasts after UVA irradiation were detected using DCFH-DA. The results are shown in Table 1.

[0050] In the experiment, the UVA irradiation dose was 30 J / cm². 2The blank control group consisted of cells not exposed to UVA, the negative control group consisted of cells exposed to UVA only, and the positive control group was administered 0.05% vitamin E, 0.03% panthenol, and 0.006% olive leaf extract (corresponding to 0.0022% oleuropein). The composition was 0.03% panthenol + 0.006% olive leaf extract. The concentration of the test sample was determined by cytotoxicity experiments.

[0051] Table 1: Effects of panthenol and olive leaf extract on reactive oxygen species in cells

[0052] Test group Blank control negative control Positive control Panthenol olive leaf extract Composition ROS(MFI) 27186.00 53718.53 30828.93 27630.17 33367.67 22878.40 SD 1784.33 194.62 2621.45 2865.35 1720.72 1023.44 p-value / 0.000 0.000 0.000 0.000 0.000 Inhibition rate % / / 42.60％ 48.56％ 37.88％ 57.41％

[0053] The results showed that both panthenol and olive leaf extract significantly reduced the increase in reactive oxygen species (ROS) content in fibroblasts induced by UVA irradiation. Panthenol inhibited ROS by 48.56%, olive leaf extract by 37.88%, and the combined effect of panthenol and olive leaf extract inhibited ROS by 57.41%. The p-values ​​for the inhibition rates of the combined effect compared to the panthenol group (0.049) and the combined effect compared to the olive leaf extract group (0.001) were statistically significant. This indicates that the combined effect can alleviate UV-induced cellular oxidative stress and exhibits a synergistic effect.

[0054] Example 3: Composition reduces oxidative stress in the skin

[0055] The effects of olive leaf extract, panthenol, and their combination on ROS content in an epidermal model after UVB irradiation were detected using a fluorescence colorimetric method. The results are shown in Table 2 and 3. Figure 1 .

[0056] In the experiment, the UVB irradiation dose was 600 mJ / cm². 2 The blank control group was an epidermal model without UVB irradiation, the negative control group was an epidermal model irradiated only by UVB, and the positive control group was given 7 μg / mL vitamin E, 1% panthenol, and 0.2% olive leaf extract (corresponding to 0.075% oleuropein content). The composition was 1% panthenol + 0.2% olive leaf extract.

[0057] Table 2: Effects of panthenol and olive leaf extract on skin reactive oxygen species

[0058]

[0059]

[0060] The results showed that both panthenol and olive leaf extract significantly reduced the increase in reactive oxygen species (ROS) content in the epidermal model induced by UVB irradiation. Panthenol inhibited ROS by 19.31%, olive leaf extract by 17.24%, and the combined group of panthenol and olive leaf extract inhibited ROS by 22.76%. The p-value for the inhibition rate between the combined group and the olive leaf extract group was 0.032, indicating a significant difference. This suggests that the combined group can alleviate UV-induced skin oxidative stress and exhibits a synergistic effect.

[0061] Example 4: Protective effect of the composition against ultraviolet radiation

[0062] To verify the photoprotective effect of the embodiments on the skin, the present invention tested the effects of olive leaf extract (0.2%, corresponding to an oleuropein concentration of 740 ppm), panthenol (1%), and the combination (0.2% olive leaf extract + 1% panthenol) on the epidermal model exposed to ultraviolet (UVB) radiation, and analyzed the soothing and repairing effects of single components and the combination on UV-induced skin damage.

[0063] The full-thickness epidermal reconstruction model EpiDermFT (purchased from MatTek, USA) was treated with 200 μL of the corresponding test substance for 4 hours and then irradiated with UVB (280-340 nm, 200 mJ / cm²). 2 After incubation, the model was treated with the corresponding test substance for 24 hours. Following incubation, the model surface was cleaned with sterile PBS solution to remove any remaining test substance, and residual liquid inside and outside the model was wiped away with sterile cotton swabs. The epidermal model was cut in half. One half was perforated, and total RNA was extracted for microarray analysis (Affymetrix Human Clariom S, 21448 genes). The other half was fixed in 10% formalin, embedded in paraffin, trimmed, and sectioned for H&E staining (Hematoxylin and eosin stain) to analyze cell and tissue morphological changes. Each group included three parallel experiments.

[0064] Ultraviolet (UV) radiation can damage cells and induce cell death by disrupting the structure of genetic material within keratinocytes and affecting apoptosis signaling pathways. These apoptotic cells are called "sunburn cells," characterized by condensed nuclei and eosinophilic cytoplasm (Laethem, AV et al., The sunburn cell: regulation of death and survival of the keratinocyte. Int J Biochem Cell Biol 37, 1547-1553 (2005)). Their quantity can be used to quantify the damage caused by UV radiation to the skin.

[0065] H&E staining results are shown in […]. Figure 2 Compared to the negative control, the blank control showed that UVB caused a thinning of the skin's viable cell layer (dark tissue) and an increase in the number of sunburned cells (white triangles pointing inwards, characterized by shrunken nuclei and darker staining). Compared to the negative control, olive leaf extract slightly increased the thickness of the skin's viable cell layer and reduced the number of sunburned cells by 40%. Compared to the negative control, panthenol showed no significant change in the thickness of the skin's viable cell layer and reduced the number of sunburned cells by 32%. Compared to the negative control, the combined composition resulted in a thicker viable cell layer, approaching the level of normal skin tissue, and a 62% reduction in the number of sunburned cells. Therefore, the combination of olive leaf extract and panthenol is more effective than either ingredient in repairing tissue morphology damage and reducing cell damage.

[0066] Example 5: GO enrichment analysis of gene expression in the epidermal model of the applied composition

[0067] To explain the synergistic effect of the combination of olive leaf extract and panthenol in repairing UV damage, gene microarray sequencing was used to analyze the effects of olive leaf extract, panthenol, and the combination on gene expression levels in a skin model.

[0068] From the microarray analysis results, genes with a p-value less than 0.05 (significant change) in the experimental group compared to the negative control group were subjected to GO enrichment analysis for differentially expressed genes. The GO enrichment analysis results for upregulated and downregulated genes are shown below. Figure 3 and Figure 4 Following application of the combination of panthenol and olive leaf extract, upregulated genes were associated with processes including heat shock protein binding, ATP-related activity regulation, serine / threonine protein phosphatase activity, cAMP-mediated signal transduction, and regulation of internal apoptosis signaling pathways. Downregulated genes were associated with processes including vitamin D response, activation of cysteine ​​endopeptidase activity involved in apoptosis, negative regulation of cellular components and tissues, and inflammasome complexes. Therefore, the repair effect of the combination of olive leaf extract and panthenol on UV damage may be related to reducing cell apoptosis, enhancing skin barrier structure, and inhibiting inflammatory responses.

[0069] Example 6: Changes in the expression levels of cellular process-related genes in an epidermal model treated with the composition.

[0070] From the microarray analysis results, genes that showed a p-value less than 0.05 (significant change) in the experimental group compared to the negative control group and were related to cell processes were screened out. The results are shown in Table 3.

[0071] Table 3: Expression levels of cellular process-related genes obtained from microarray analysis

[0072] Gene Panthenol olive leaf extract Composition FASTKD3 +103.03％ +105.39％ +123.91％ SELE -89.21％ -83.55％ -89.78％ CASP10 -900％ -908％ -1097％

[0073] FASTKD3 encodes part of the structure of a Fas-activated serine / threonine kinase, regulated by mitochondrial stress, and controls apoptosis (Simarro et al., Fast kinase domain-containing protein 3 is amitochondrial protein essential for cellular respiration. Biochem Biophys ResCommun. 401, 440-446 (2010)). SELE encodes selectin E, a glycoprotein expressed upon activation by inflammatory factors or endotoxins, mediating leukocyte adhesion to the endothelium and playing an important role in local inflammation. CASP10 encodes a cysteine-aspartic protease that induces apoptosis under stress conditions.

[0074] UVB stimulation led to a decrease in FASTKD3 expression. After application of the test sample, panthenol caused a 103.03% increase in expression, olive leaf extract caused a 105.39% increase, and the combination caused a 123.91% increase. UVB caused a significant 41-fold increase in SELE expression, which decreased by 89.21% after application of panthenol, 83.55% after application of olive leaf extract, and 89.78% after application of the combination. UVB caused an increase in CASP10 expression, which decreased significantly after application of the test sample. Panthenol caused a 900% decrease in expression, olive leaf extract caused a 908% decrease, and the combination caused a 1097% decrease.

[0075] Therefore, the combination of olive leaf extract and panthenol has a synergistic effect in regulating changes in gene expression levels in cellular processes induced by UVB stimulation, thereby reducing apoptosis caused by external stimuli.

[0076] Example 7: The composition promotes the expression of barrier-related genes in an epidermal model.

[0077] In addition to exhibiting a synergistic regulatory effect on the expression of genes related to the aforementioned cellular processes, the combination of olive leaf extract and panthenol significantly affects the expression of genes related to skin barrier structure and inflammation. GRHL3 encodes a granuly head protein transcription factor that affects the expression of glutamin transferase TGM1, influencing the formation of tight junctions in the skin barrier and playing an important role in maintaining the skin barrier (Deng et al., Grainyhead-like transcription factors: guardians of the skin barrier. Veterinary Dermatology 32, 553-e152 (2021)). IFNA7 encodes interferon α7, a low-molecular-weight glycoprotein produced by macrophages during the immune process, participating in the JAK / STAT signaling pathway and affecting the inflammatory response. IL15 encodes interleukin-15, inducing activation of JAK kinase, activating transcription factors STAT3 and STAT5, and regulating the inflammatory response. The CXCL10 gene encodes a chemokine, also known as interferon-gamma-induced protein 10 (IP-10), which mainly induces chemotactic responses in monocytes and macrophages, recruits effector cells into inflammatory sites, participates in regulating the migration, activation, and differentiation of immune cells, and affects immune and inflammatory processes.

[0078] After application of the combination of panthenol and olive leaf extract, the expression levels of GRHL3 increased by 29.08%, IFNA7 decreased by 88.52%, IL15 decreased by 82.32%, and CXCL10 decreased by 64.32% in the full-thickness epidermal model.

[0079] In conclusion, the combination of panthenol and olive leaf extract can repair UVB-induced skin damage by strengthening the skin barrier structure and inhibiting inflammatory responses.

[0080] Example 8 investigated the differences in the effect of different antioxidants on the stability improvement of olive leaf extract.

[0081] The olive leaves are sourced from Longnan, Gansu. The olive leaf extract was purified using macroporous resin adsorption, and the extract is a solution containing more than 0.6% oleuropein.

[0082] Macroporous resin adsorption method: Dried olive leaves were pulverized and added to a 70% ethanol aqueous solution, then heated under reflux three times. The extracts were combined and filtered to obtain a concentrated extract. The extract was then used to adsorb the active ingredients using a macroporous resin column. After adsorption, the resin column was washed with water to remove impurities, and then desorbed using a 70% ethanol aqueous solution. The desorbed solution was collected and a suitable amount of aqueous alcohol solution was added to prepare the product, olive leaf extract.

[0083] Oxothiazolidinyl carboxylic acid is an amino acid derivative, often used as a whitening active ingredient in cosmetics. It has good antioxidant effects and was purchased from Nanjing Spaco Biochemical Industry Co., Ltd.

[0084] Pentaerythritol tetra(bis-tert-butylhydroxyhydrocinnamic acid) ester is a cinnamic acid derivative and a good antioxidant, purchased from BASF (China) Co., Ltd.

[0085] (Tris(tetramethylhydroxypiperidinol) citrate is a derivative of tetramethylpiperidine and is a high-performance light stabilizer, purchased from BASF (China) Co., Ltd.

[0086] Glucosylrutin is a derivative of rutin and also has extremely strong antioxidant effects. It was purchased from Xianting.

[0087] Diethylhexyl eugenol malonate is a light stabilizer purchased from Merck Chemical Technology Co., Ltd.

[0088] First, this study investigated whether oxothiazolidinyl carboxylic acid could improve the stability of olive leaf extract compared to commonly available antioxidants such as pentaerythritol tetra(bis-tert-butylhydroxyhydrocinnamic acid), glucosylrutin, tris(tetramethylhydroxypiperidinol) citrate, and diethylhexyl eugenol malonate. Ten samples (groups 1-10) were used to study the differences in the stability improvement of olive leaf extract by different antioxidants, and the component ratios are shown in Table 4. Emulsion samples were prepared using industry-standard methods according to the following formulation, wherein the olive leaf extract contained 0.6% oleuropein.

[0089] Table 4: Composition ratio of groups 1-10

[0090]

[0091]

[0092] Antioxidants in groups 1-10 include different concentrations of oxothiazolidine carboxylic acid, pentaerythritol tetra(bis-tert-butylhydroxyhydrocinnamic acid) ester, glucosylrutin, tris(tetramethylhydroxypiperidinol) citrate, diethylhexyl syringite malonate and their combinations.

[0093] The color changes of Examples 1-10 were observed after 10 days of storage under different temperature conditions (-18℃, 4℃, RT, 48℃; some samples were not tested at room temperature). The results showed that under high temperature conditions, groups 3-10 all exhibited a significant deepening of color, indicating that pentaerythritol tetra(bis-tert-butylhydroxyhydrocinnamic acid) ester, glucosylrutin, tris(tetramethylhydroxypiperidinol) citrate, and diethylhexyl syringite malonate failed to improve the stability of the olive leaf extract. Groups 1 and 2 showed less color change than the other groups under high temperature conditions, suggesting that oxothiazolidinyl carboxylic acid has a certain effect on improving the stability of the olive leaf extract. Figure 5 The color changes of groups 1-10 after being placed at different temperatures for 10 days are listed.

[0094] The conclusions are as follows:

[0095] Pentaerythritol tetra(bis-tert-butylhydroxyhydrocinnamic acid) ester, glucosylrutin, tris(tetramethylhydroxypiperidinol) citrate, and diethylhexyl syringite malonate all failed to improve the stability of olive leaf extract. However, oxothiazolidinyl carboxylic acid had a certain effect on improving the stability of olive leaf extract.

[0096] Example 9: Method for investigating the effect of oxothiazolidinic acid on the stability of olive leaf extract.

[0097] To further investigate the effect of oxothiazolidine carboxylic acid on the stability of olive leaf extract, groups 11-18 were used to study the effects of temperature (4℃ and 40℃, respectively) and aqueous solution pH (5.5 and 6, respectively) on solution color. The component composition is shown in Table 5. The content of oleuropein in the olive leaf extract was greater than 1%.

[0098] Table 5: Composition ratio of groups 11-18

[0099] Element Group 11 Group 12 Group 13 Group 14 water Up to 100 Up to 100 Up to 100 Up to 100 Butylene glycol 10 10 10 10 1,2-Pentanediol 4 4 4 4 Panthenol 1 1 1 1 olive leaf extract 20 20 20 20 oxothiazolidinic acid - 0.15 - 0.15 temperature 4℃ 4℃ 40℃ 40℃ pH 5.5 5.5 5.5 5.5 Element Group 15 Group 16 Group 17 Group 18 water Up to 100 Up to 100 Up to 100 Up to 100 Butylene glycol 10 10 10 10 1,2-Pentanediol 4 4 4 4 Panthenol 1 1 1 1 olive leaf extract 20 20 20 20 oxothiazolidinic acid - 0.15 - 0.15 temperature 4℃ 4℃ 40℃ 40℃ pH 6 6 6 6

[0100] After adjusting the pH to the specified value using trace amounts of arginine and citric acid, groups 11-18 were placed in refrigerators or ovens at corresponding temperatures. The color changes of the solutions were observed on days 7, 14, and 28. In samples without the added antioxidant oxothiazolidinic acid, the solution color deepened with increasing time, temperature, and pH. Samples with added oxothiazolidinic acid were significantly lighter in color compared to samples without oxothiazolidinic acid under the same treatment conditions, indicating that oxothiazolidinic acid has a certain protective effect on olive leaf extract, slowing down its oxidative discoloration. Figure 6 The color changes of groups 11-18 after being placed at different temperatures for 10 days are listed.

[0101] The conclusions are as follows:

[0102] The solution color deepened more rapidly with increasing time, temperature, and pH. The sample with added oxothiazolidinic acid was significantly lighter in color compared to the sample without oxothiazolidinic acid under the same treatment conditions, indicating that oxothiazolidinic acid has a protective effect on olive leaf extract, slowing down its oxidative discoloration.

[0103] Example 10: Study on the effect of oxothiazolidine carboxylic acid content on the stability of olive leaf extract.

[0104] The color changes of olive leaf extract with an oxothiazolidine carboxylic acid content of 0.3% were studied using a similar testing method to those described in the above embodiments. These results were obtained under different temperatures (4℃, 40℃) and aqueous solution pH values ​​(5, 5.5, 6). The component distributions are shown in Table 6, and the color change results are as follows: Figure 6 As shown in the figure, the content of oleuropein in the olive leaf extract is greater than 1%.

[0105] Table 6: Composition ratio of groups 19-30

[0106]

[0107]

[0108] In the sample without the added antioxidant oxothiazolidinic acid, the solution color was observed to darken with increasing time, temperature, and pH. The sample with added oxothiazolidinic acid was significantly lighter in color compared to the sample without oxothiazolidinic acid under the same treatment conditions, indicating that oxothiazolidinic acid has a certain protective effect on olive leaf extract, slowing down its oxidative discoloration. Figure 7 The color changes of groups 19-30 after being placed at different temperatures for 10 days are listed.

[0109] The conclusions are as follows:

[0110] contrast Figure 6 and Figure 7 According to the corresponding samples (groups 14 and 26, groups 18 and 30), increasing the concentration of oxothiazolidine carboxylic acid can further slow down the oxidative discoloration of olive leaf extract.

[0111] Example 11: Study on the effect of oxothiazolidinic acid on the degradation of oleuropein.

[0112] The most important active ingredient in olive leaf extract is oleuropein. Oleuropein is easily degraded by environmental changes such as light, acid, alkali, and enzymes, affecting its biological activity. The content changes of oleuropein in groups 11-18 over 14 days were tested by HPLC, and the results are shown in Table 7.

[0113] Table 7: Changes in the content of oleuropein in groups 11-18 over 14 days

[0114]

[0115]

[0116] Comparing the degradation rates of oleuropein in groups 11-18 under different aqueous solution conditions, it was found that increasing the pH and temperature of the aqueous solution both enhance the degradation rate of oleuropein. In groups 11-18, the addition of oxothiazolidinic acid did not affect the degradation of oleuropein on day 7; on day 14, under low temperature conditions, the addition of oxothiazolidinic acid had no effect on the degradation rate of oleuropein, while under high temperature conditions, in aqueous solutions with pH=5.5 and pH=6, oxothiazolidinic acid reduced the degradation rate of oleuropein. This indicates that the degradation rate of oleuropein is mainly affected by the aqueous solution conditions, but under high temperature conditions, oxothiazolidinic acid can reduce the degradation rate of oleuropein to some extent.

[0117] The conclusions are as follows:

[0118] Oxothiazolidinic acid can significantly improve the discoloration problem of olive leaf extract and can slow down the degradation of oleuropein, the main active ingredient in olive leaf extract, to some extent. Adding oxothiazolidinic acid can enhance the stability of olive leaf extract and expand its application range in cosmetic formulations.

[0119] The above description is merely a specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent modifications or substitutions that can be easily conceived by those skilled in the art within the scope of the technology disclosed in the present invention should be included within the scope of protection of the present invention.

Claims

1. The use of a repairing composition in the preparation of cosmetics that enhance the skin barrier structure, characterized in that, The repair composition comprises panthenol and olive leaf extract, wherein oxothiazolidin carboxylic acid is added to the olive leaf extract to increase stability; the ratio of oxothiazolidin carboxylic acid to oleuropein in the olive leaf extract is (0.01-10):1, and the ratio of panthenol to oleuropein in the olive leaf extract in the repair composition is 1:(0.001-1) by weight.

2. A repair composition, characterized in that, The composition comprises panthenol and olive leaf extract, wherein oxothiazolidin carboxylic acid is added to the olive leaf extract to increase stability; the ratio of oxothiazolidin carboxylic acid to oleuropein in the olive leaf extract is (0.01-10):1, and the ratio of panthenol to oleuropein in the repair composition is 1:(0.001-1) by weight.

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