A pesticide combination containing prothioconazole and its application

Abstract

The present invention relates to the field of pesticides and fungicides, and specifically discloses a pesticide composition containing prothioconazole. The active ingredients of the pesticide composition include a fungicide and an insecticide, wherein the fungicide includes prothioconazole and trifloxystrobin, and the insecticide is thiamethoxam. The mass ratio of the fungicide prothioconazole to trifloxystrobin is 1:10 to 10:1, and the mass ratio of the fungicide to the insecticide is 1:1 to 5:1. The pesticide composition of the present invention can treat both rice diseases and insect pests, and has a particularly good synergistic effect against rice bakanae disease and rice thrips. A single application of the pesticide provides significant control efficacy, reduces the amount of pesticide used, and reduces toxicity.

Description

Technical Field

[0001] The present invention relates to the field of pesticides and fungicides, and in particular to a pesticide composition containing prothioconazole and application thereof. Background Art

[0002] Prothioconazole is a trithione fungicide discovered, developed, and produced by Bayer CropScience. Its mechanism of action is to inhibit the demethylation of lanosterol or 2,4-methylenedihydrolanosterol, the precursors of sterols in fungi, at the 14-position. Prothioconazole not only has systemic activity, but also has excellent protective, therapeutic, and eradication activities, and has a significant yield-increasing effect. Compared with triazole fungicides, prothioconazole has a broader spectrum of fungicidal activity. Prothioconazole has low toxicity, is non-teratogenic, non-mutagenic, non-toxic to embryos, and is safe for humans and the environment. It is mainly used to control numerous diseases of crops such as cereals, wheat, and legumes. It has excellent control effects on almost all wheat diseases, such as powdery mildew, sheath blight, wilt, leaf spot, rust, sclerotinia, net blotch, and moire. Its structural formula is as follows:

[0003]

[0004] Penflufen (ISO common name: penflufen; chemical name: N-[2-(1,3-dimethylbutyl)phenyl]-5-fluoro-1,3-dimethyl-1H-pyrazole-4-carboxamide; CAS number: 494793-67-8) is a pyrazoleamide fungicide developed by Bayer. It is a succinate dehydrogenase inhibitor that acts on electron transport complex II of the respiratory chain, blocking energy metabolism. It is primarily used for seed treatment. Treated seeds absorb penflufen during germination and transmit it through the xylem to other parts of the plant, thereby protecting the crop. It can control a variety of diseases, including potato black spot, wheat sheath blight, rice sheath blight, and rapeseed sclerotinia.

[0005] Thiamethoxam, a second-generation neonicotinoid insecticide with a completely new structure, has contact, stomach, and systemic effects on a wide range of pests and can be used for foliar spraying and seed treatment. With recent developments, thiamethoxam and other neonicotinoid insecticides are often applied as seed treatments to control aboveground plant pests.

[0006] With the widespread application of green pest control technologies for crops, seed coating has become an important measure for controlling seedling pests and diseases, promoting growth and high yields. The inventors of this invention, through laboratory and field trials, have discovered that mixing the fungicides prothioconazole and penflufenacil with the insecticide thiamethoxam in a specific ratio for seed treatment has a significant synergistic effect against rice diseases and pests, effectively controlling both diseases and pests with a single application. Summary of the Invention

[0007] Based on the above situation, the purpose of the present invention is to provide a pesticide composition containing prothioconazole, which is mainly used for controlling plant diseases and insect pests, especially rice diseases and insect pests. The composition can enhance the efficacy, reduce the dosage of the medicine, and at the same time prolong the lasting effect and delay the development of drug resistance.

[0008] In order to achieve the above object, the present invention adopts the following technical solution: a pesticide composition containing prothioconazole, wherein the active ingredients of the pesticide composition include a fungicide and an insecticide, the fungicide includes prothioconazole and trifloxystrobin, and the insecticide is thiamethoxam;

[0009] Furthermore, the mass ratio of the fungicide prothioconazole to pentofenac is 1:10 to 10:1;

[0010] Furthermore, the mass ratio of the fungicide prothioconazole to pentoxafen is 1:10, 1:8, 1:4, 1:2, 1:1, 2:1, 4:1, 8:1, and 10:1;

[0011] Furthermore, the mass ratio of prothioconazole to pentoxafen is 1:8 to 8:1;

[0012] Furthermore, the mass ratio of the fungicide prothioconazole to pentoxafen is 1:8, 1:4, 1:2, 1:1, 2:1, 4:1, and 8:1;

[0013] More preferably, the mass ratio of prothioconazole to pentoxafen is 1:8 to 4:1;

[0014] Furthermore, the mass ratio of prothioconazole to pentoxafen is 1:8, 1:4, 1:2, 1:1, 2:1, and 4:1;

[0015] Furthermore, the mass ratio of the fungicide to the insecticide is 1:1 to 5:1;

[0016] Furthermore, the mass ratio of the fungicide to the insecticide is 1:2 to 1:50;

[0017] Furthermore, the mass ratio of the fungicide to the insecticide is 2:5 to 2:45;

[0018] Furthermore, the mass ratio of the fungicide to the insecticide is 2:5, 2:15, 2:25, 2:35, 2:45;

[0019] Furthermore, based on the total weight of the pesticide composition being 100 wt%, the total content of the fungicide in the pesticide composition is 0.5% to 50%;

[0020] Furthermore, based on the total weight of the pesticide composition being 100 wt%, the total content of the fungicide in the pesticide composition is 2% to 20%;

[0021] Furthermore, based on the total weight of the pesticide composition being 100 wt%, the content of the insecticide in the pesticide composition is 1% to 80%;

[0022] Furthermore, based on the total weight of the pesticide composition being 100 wt%, the content of the insecticide in the pesticide composition is 2% to 60%;

[0023] Furthermore, the content of the insecticide in the pesticide composition is 5%, 15%, 25%, 35%, or 45%;

[0024] Furthermore, the pesticide composition comprises, in addition to the active ingredient, auxiliary ingredients, wherein the auxiliary ingredients are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoaming agents, solvents, preservatives, stabilizers, warning colors, film formers, synergists and carriers;

[0025] The wetting agent is selected from one or more of alkylbenzene sulfonate, alkylnaphthalene sulfonate, lignin sulfonate, sodium lauryl sulfate, sodium dioctyl sulfosuccinate, α-olefin sulfonate, alkylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, alkylphenol ethoxylate, fatty alcohol ethoxylate, fatty alcohol polyoxyethylene ether sodium sulfate, silkworm feces, soapberry powder, soapberry powder, SOPA, detergent, emulsifier 2000 series and wetting penetrant F; and / or

[0026] The dispersant is selected from one or more of lignin sulfonate, alkylnaphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, tristyrylphenol ethoxylate phosphate, fatty alcohol ethoxylate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether methyl ether condensate sulfate, fatty amine polyoxyethylene ether, glycerol fatty acid ester polyoxyethylene ether, polycarboxylates, polyacrylic acids, phosphates, EO-PO block copolymers and EO-PO graft copolymers; and / or

[0027] The emulsifier is selected from one or more of calcium dodecylbenzenesulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenylethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrylphenol polyoxyethylene ether, castor oil polyoxyethylene ether and alkylphenol ether phosphate; and / or

[0028] The thickener is selected from one or more of xanthan gum, organobentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose and white carbon black; and / or

[0029] The disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid and tartaric acid; and / or

[0030] The antifreeze agent is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons and inorganic salts; and / or

[0031] Defoaming agent selected from C 10 -C 20 Saturated fatty acid compounds, silicone oil, silicone compounds, C8-C 10 One or more of fatty alcohols; and / or

[0032] The solvent is selected from one or more of benzene, toluene, xylene, durene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, alkylene carbonate, diesel, solvent oil, vegetable oil, vegetable oil derivatives and water; and / or

[0033] The preservative is selected from one or more of propionic acid, sodium propionic acid, sorbic acid, sodium sorbic acid, potassium sorbic acid, benzoic acid, sodium benzoic acid, sodium p-hydroxybenzoic acid, methyl p-hydroxybenzoate, kasone and 1,2-benzisothiazolin-3-one; and / or

[0034] The stabilizer is selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, bentonite, attapulgite, white carbon black, talc, montmorillonite and starch; and / or

[0035] The warning color is selected from any one or more adjustment colors of blue, green, red, and purple; and / or

[0036] The film-forming agent is selected from one or more of sodium carboxymethyl starch, cellulose derivatives (sodium carboxymethyl cellulose, sodium alginate, polyvinyl alcohol), and polyacrylic acid; and / or

[0037] Synergists are selected from synergist, piperonyl butoxide; and / or

[0038] The carrier is selected from one or more of ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils and vegetable oil derivatives;

[0039] Furthermore, the pesticide composition can be prepared into any formulation permitted in agriculture;

[0040] Furthermore, the formulation is any one of a solid seed treatment formulation and / or a liquid seed treatment formulation;

[0041] Furthermore, the formulation is in the form of any one of a seed treatment dry powder, a seed treatment dispersible powder, a seed treatment liquid, a seed treatment emulsion and / or a seed treatment suspension;

[0042] Furthermore, the formulation is in the form of a seed treatment suspension.

[0043] The present invention also discloses the use of the above pesticide composition in preventing and controlling plant diseases and / or insect pests;

[0044] Furthermore, the plants include corn, wheat and / or rice;

[0045] Furthermore, the plant is rice;

[0046] Furthermore, the rice diseases are rice bakanae, rice blast, rice false smut and / or rice seedling rot;

[0047] Furthermore, the rice disease is rice bakanae disease;

[0048] The rice pests are rice thrips, rice borers, rice hesperides, rice planthoppers, rice leaf rollers and / or rice water weevils;

[0049] Furthermore, the rice pest is rice thrips.

[0050] Compared with the prior art, the technical solution of the present invention has the following beneficial effects:

[0051] 1) The pesticide composition of the present invention can treat rice diseases and insect pests, especially rice bakanae disease and rice thrips, and has a good synergistic effect. A single application of the pesticide can achieve significant control effects, reduce the amount of pesticide used, and reduce toxicity.

[0052] 2) The pesticide composition of the present invention compounds fungicides and insecticides with different mechanisms of action, thereby expanding the control spectrum and slowing down the development of drug resistance in target pests and pathogenic microorganisms. DETAILED DESCRIPTION

[0053] In order to make the technical solutions, objectives and advantages of the present invention more clearly understood, the present invention is described with reference to the following specific embodiments. However, the present invention can be implemented in various forms and should not be limited to the embodiments described herein.

[0054] Preparation example:

[0055] Preparation process of seed treatment suspension: Active ingredients, adjuvants and water are mixed and stirred evenly through high shear according to the ratio, and then sanded for 2.5 hours in a sander to make the average particle size reach 1-5 microns. Finally, thickeners, preservatives and film-forming agents are added and continued to be sheared and stirred evenly to obtain seed treatment suspension.

[0056] Preparation Example 1: 17% Thiamethoxam·Pyclofosanilide·Prothioconazole Seed Treatment Suspension Concentrate (15%+1%+1%)

[0057] Calculated by weight percentage, 15% thiamethoxam, 1% fluopicolide, 1% prothioconazole, 3% alkylphenol polyoxyethylene ether, 4% styrylphenol polyoxyethylene ether phosphate, 2% sodium lignin sulfonate, 2% polyacrylic acid, 0.25% xanthan gum, 5% rose red pigment, 5% ethylene glycol, 1% magnesium aluminum silicate, 0.5% silicone defoamer, 1% sodium benzoate, and deionized water to make up the balance.

[0058] Preparation Example 2: 27% Thiamethoxam·Pyclofosanilide·Prothioconazole Seed Treatment Suspension Concentrate (25%+1%+1%)

[0059] Calculated by weight percentage, 25% thiamethoxam, 1% prothioconazole, 1% fluopicolide, 1% isotridecyl alcohol polyoxyethylene ether, 3% alkylphenol polyoxyethylene ether phosphate, 1% polycarboxylic acid sodium salt, 2% sodium carboxymethyl cellulose, 0.3% xanthan gum, 5% rose red pigment, 1% magnesium aluminum silicate, 0.5% silicone defoaming agent, 2% sodium sorbate, 5% propylene glycol, 2% polyacrylic acid, and deionized water to make up the balance.

[0060] Preparation Example 3: 37% Thiamethoxam·Pyclofosanilide·Prothioconazole Seed Treatment Suspension Concentrate (35%+1%+1%)

[0061] Calculated by weight, the following ingredients: 35% thiamethoxam, 1% prothioconazole, 1% fluopicolide, 3% fatty alcohol ethylene oxide-propylene oxide copolymer, 2% fatty amine polyoxyethylene ether, 2% sodium lignin sulfonate, 3% tristyrylphenol ethoxylate phosphate, 2% polyacrylic acid, 0.2% xanthan gum, 6% rose red pigment, 6% glycerol, 1% magnesium aluminum silicate, 0.5% silicone defoamer, 2% methyl parahydroxybenzoate, and deionized water to make up the balance.

[0062] Indoor activity test:

[0063] Example 1: Indoor activity test of Fusarium moniliforme

[0064] Test basis: The test refers to NY / T 1156.2-2006 "Guidelines for Indoor Bioassay of Pesticides - Fungicides Part 2: Plate Method for Inhibition of Pathogenic Fungal Mycelial Growth".

[0065] Test strain: Fusarium moniliforme, provided by the R&D Center of Hailier Pharmaceutical Group. The strain was activated and stored at 4°C for future use.

[0066] Test agents: 97% prothioconazole technical, 95% pentoconazole technical, 98% thiamethoxam technical, all of which are provided by the R&D center of Hailier Pharmaceutical Group.

[0067] Test medium: The test medium was potato dextrose agar (PDA).

[0068] Other reagents: acetone (analytical grade), Tween 80 (chemical grade), all of which are commercially available.

[0069] Preparation of the agent: Prothioconazole, trifloxystrobin and thiamethoxam technical drugs were dissolved in acetone to prepare high-concentration stock solutions. Then, prothioconazole: trifloxystrobin were prepared in the ratio of 1:10, 1:8, 1:4, 1:2, 1:1, 2:1, 4:1, 8:1 and 10:1 to prepare 9 mixed solutions. Then, the above single agents and mixed solutions were diluted with 0.1% Tween 80 aqueous solution to the required series of mass concentrations.

[0070] On this basis, a better ratio of prothioconazole and fluopicolide was screened out, and the better ratio mixture was used as a fungicide combination and was compounded with thiamethoxam in different mass ratios.

[0071] Test repetition: 4 culture dishes were used for each concentration of the test drug, 1 culture dish was used for each repetition, and a total of 4 repetitions were performed. A 0.1% Tween 80 aqueous solution without drug was used as a blank control.

[0072] Preparation of drug-containing culture medium: In a clean bench, add pre-sterilized, thawed culture medium to sterile Erlenmeyer flasks, adding 72 mL to each flask. Cool to approximately 45°C. Then, using a pipette, quantitatively pipette 8 mL of each prepared drug into each sterile Erlenmeyer flask in ascending concentration order. Shake thoroughly and pour evenly into four 9 cm diameter Petri dishes to prepare drug-containing PDA plates of the corresponding concentrations. Each treatment was replicated four times. A blank control was prepared using a 0.1% Tween 80 aqueous solution without drug.

[0073] Inoculation: Cut the pre-cultured Fusarium moniliforme from the edge of the colony with a sterile punch under sterile conditions, inoculate the cake into the center of the drug-containing plate with an inoculator, cover it with a cap, and place it in a constant temperature incubator at 25°C for culture.

[0074] Experimental investigation: Investigate the growth of pathogenic fungi based on the growth of colonies in the blank control culture dish. Measure the colony diameter (cm) with a ruler. Measure the diameter of each colony once using the cross-hatch method and take the average value.

[0075] Data statistics and analysis: Based on the survey results, calculate the mycelial growth inhibition rate of each treatment concentration on the target bacteria, the unit is percentage (%), and the calculation results are rounded to two decimal places.

[0076] D=D1-D2

[0077] Where:

[0078] D——colony growth diameter;

[0079] D1 - colony diameter;

[0080] D2——diameter of mushroom cake.

[0081]

[0082] Where:

[0083] I——hyphae growth inhibition rate;

[0084] D0——blank control colony growth diameter;

[0085] D T ——The growth diameter of the colony after treatment with chemicals.

[0086] Sun Yunpei method: The synergistic effect of mixed drugs is evaluated based on the co-toxicity coefficient (CTC). A co-toxicity coefficient of CTC ≥ 120 indicates a synergistic effect; CTC ≤ 80 indicates an antagonistic effect; and 80 < CTC < 120 indicates an additive effect.

[0087] Calculation of the co-toxicity coefficient (CTC value) of the mixture:

[0088]

[0089] Where:

[0090] ATI - measured toxicity index of mixture;

[0091] S——EC of standard agent 50 , the unit is milligrams per liter (mg / L);

[0092] M——EC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0093] TTI=TI A *P A +TI B *P B

[0094] Where:

[0095] TTI – Theoretical Toxicity Index of Mixtures;

[0096] TI A ——Agent toxicity index;

[0097] P A——The percentage of agent A in the mixture, in percentage (%);

[0098] TI B ——Toxicity index of agent B;

[0099] P B ——The percentage of agent B in the mixture, in percentage (%).

[0100]

[0101] Where:

[0102] CTC – Co-toxicity coefficient;

[0103] ATI - measured toxicity index of mixture;

[0104] TTI - Theoretical Toxicity Index of Mixture.

[0105] DPS data processing software was used to calculate the test results, determine the co-toxicity coefficient (CTC) of different drug ratios, and screen out the optimal ratio of the test drugs. The raw data of all replicates for each treatment were recorded.

[0106] The test results are shown in the table below:

[0107] Table 1 Results of indoor toxicity test of prothioconazole, trifloxystrobin and their mixtures against Fusarium moniliforme

[0108]

[0109] There are differences in the mechanism of action of prothioconazole and fluopicolide. The results of indoor tests show that the combination of the two compounds is beneficial to improve the control effect. Prothioconazole and fluopicolide have good control effects on Fusarium moniliforme. Their EC 50 4.1149 mg / L -1 , 4.6094mg.L -1 The mass ratio of prothioconazole to fluopicolide showed synergistic effect in the range of 1:8 to 8:1, among which the mass ratio of prothioconazole to fluopicolide was 1:1, which had the best activity. 50 It is 2.5338 mg / L, and the co-toxicity coefficient is 171.605.

[0110] Table 2 Results of indoor toxicity test of prothioconazole + trifloxystrobin (1:1) mixture and its mixture with thiamethoxam against Fusarium moniliforme

[0111]

[0112] From the indoor activity test in Table 2, it can be seen that thiamethoxam has no obvious activity against Fusarium moniliforme. Its EC 50Higher than 200mg.L -1 The mass ratio of prothioconazole to fluopicolide was 1:1 when mixed with thiamethoxam, which showed a synergistic effect on Fusarium moniliforme and a good inhibitory effect on the mycelial growth of Fusarium moniliforme.

[0113] Example 2: Indoor activity test of rice thrips

[0114] Test basis: The test was carried out in accordance with NY / T 1154.8-2007 Agricultural Industry Standard of the People's Republic of China "Guidelines for Indoor Bioassay Tests of Pesticides - Insecticides Part 8: Filter Paper Film Method".

[0115] Test target: Rice thrips (Chloethrips oryzae), the insect source was collected from the rice fields of Lancun, Jimo, Qingdao, Shandong Province, and cultured indoors for three generations at the R&D center of Hailier Pharmaceutical Group for future use.

[0116] Test agents: 95% prothioconazole technical, 95% fluopicolide technical, 98% thiamethoxam technical, all of which were provided by the R&D center of Hailier Pharmaceutical Group.

[0117] Other reagents: acetone (analytical grade), Tween 80 (chemical grade).

[0118] Preparation: Dissolve the original drug in acetone, then dilute with a 0.1% Tween-80 solution. Prepare separate stock solutions and design appropriate ratios based on the intended purpose and the drug's activity. Each single dose and each mixture is prepared in equal proportions to achieve the desired concentration.

[0119] Preparation of drug film: prepare a culture dish with a diameter of 9 cm, take a filter paper with a diameter of 9 cm and place it on the bottom of the dish, respectively take 1 mL of the prepared drug solution of each concentration and evenly add it on the filter paper, wait for the solvent to evaporate to form a drug film, and leave it for 24 hours for use.

[0120] Treatment method: Use a brush to select 15 test insects with the same physiological state, move them into a culture dish lined with filter paper film, and after the test insects crawl on the filter paper film for 1 hour, transfer them to a clean culture dish with clean feed for feeding.

[0121] Rearing and observation: The treated test insects were cultured in an artificial climate chamber at 25±1℃, relative humidity 60%-80%, and photoperiod L:D=16h:8h.

[0122] Test investigation: 72 hours after the treatment, the death of the test insects was investigated. The criterion for judging the death of the test insects was obvious shrinkage of the insect body or inability to crawl normally after being pricked with a needle. The number of dead insects was recorded.

[0123] Data statistics and analysis: Based on the survey data, calculate the adjusted mortality rate of each treatment. Calculate according to formulas (1) and (2), and retain the results to two decimal places:

[0124]

[0125] Where:

[0126] P——mortality rate, in percentage (%);

[0127] K——number of dead insects, in heads;

[0128] N——Total number of insects processed, in heads.

[0129]

[0130] Where:

[0131] P1——adjusted mortality rate, in percentage (%);

[0132] P t ——Treatment mortality rate, in percentage (%);

[0133] P0 - blank control mortality rate, in percentage (%).

[0134] If the control mortality rate is less than 5%, no correction is required; if the control mortality rate is between 5% and 20%, correction should be made according to formula (2); if the control mortality rate is greater than 20%, the test needs to be repeated.

[0135] Sun Yunpei method: The synergistic effect of mixed drugs is evaluated based on the co-toxicity coefficient (CTC). A co-toxicity coefficient of CTC ≥ 120 indicates a synergistic effect; CTC ≤ 80 indicates an antagonistic effect; and 80 < CTC < 120 indicates an additive effect.

[0136] The co-toxicity coefficient (CTC value) of the mixture is calculated according to formula (3), formula (4), and formula (5):

[0137]

[0138] Where:

[0139] ATI - measured toxicity index of mixture;

[0140] S——LC of standard pesticide 50 , the unit is milligrams per liter (mg / L);

[0141] M——LC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0142] TTI=TI A *PA +TI B *P B .......(4)

[0143] Where:

[0144] TTI – Theoretical Toxicity Index of Mixtures;

[0145] TI A ——Agent toxicity index;

[0146] P A ——The percentage of agent A in the mixture, in percentage (%);

[0147] TI B ——Toxicity index of agent B;

[0148] P B ——The percentage of agent B in the mixture, in percentage (%).

[0149]

[0150] Where:

[0151] CTC – Co-toxicity coefficient;

[0152] ATI - measured toxicity index of mixture;

[0153] TTI - Theoretical Toxicity Index of Mixture.

[0154] DPS data processing software was used to calculate the test results, determine the co-toxicity coefficient (CTC) of different drug ratios, and screen out the optimal ratio of the test drugs. The raw data of all replicates for each treatment were recorded.

[0155] The test results are shown in the table below:

[0156] Table 3 Results of indoor toxicity tests on rice thrips of mixtures of prothioconazole and fluopicolide and their mixtures with thiamethoxam

[0157]

[0158]

[0159] The results of indoor activity test in Table 3 show that the mixture of prothioconazole and fluopicolide has no significant activity against rice thrips. Thiamethoxam has good activity against rice thrips. 50 9.9721 mg.L -1 The test results showed that combining a mixture of prothioconazole and fluopicolide with thiamethoxam can significantly increase the toxicity of the insecticide thiamethoxam and enhance the control effect against rice thrips.

[0160] Field efficacy trials

[0161] Example 3: Field efficacy test of compound preparation on rice seedling fusarium

[0162] Test basis: The test refers to GB / T 17980.104-2004 "Guidelines for Field Efficacy Tests of Pesticides (II) Part 104: Fungicides for Control of Rice Bakanae Disease".

[0163] Target of prevention and control: Rice seedling blight (Fusarium moniliforme).

[0164] Experimental crop: Rice (Xiushui 134).

[0165] The trial was conducted in rice paddies in Cangtou Village, Baohua Town, Jurong City, Jiangsu Province. Rice was growing normally in loam soil with a pH of 6.8 and an organic matter content of 1.9%. Cultivation conditions (soil type, water and fertilizer management, planting density, and growing period) were uniform across plots and in accordance with local Good Agricultural Practices (GAP).

[0166] Dosage of drugs: See the table below for the test drugs and dosage:

[0167] Table 4 Test agents and dosage

[0168]

[0169]

[0170] Plot arrangement: The plot treatments of test agent, control agent and blank control were arranged in random blocks.

[0171] Plot area: rice paddy plot area 10m 2 , repeated 4 times; field plot area 50m 2 , repeat 4 times.

[0172] Application method: Coat the rice seeds before sowing and sow them after they are naturally dried in the shade.

[0173] Application time and frequency: According to the experimental design, rice seeds were coated on May 30, 2019, with a total of one application. Rice seeds were sown on May 31, 2019.

[0174] Sowing: Four trays of rice (55 cm * 25 cm) were sown in each plot of the nursery field and placed in different locations of the plot. The number of seeds sown in each tray was about 3900 for the investigation of germination rate.

[0175] Experimental investigation:

[0176] 1) Seedling emergence survey: During the rice seedling emergence period, four rice trays sown at designated locations were surveyed in each plot, with half of each tray surveyed.

[0177] 2) Seedling quality survey: The seedling quality was surveyed at the 3.5-leaf stage. Sampling was conducted at 5 points in each plot, and 20 plants were randomly selected at each point to survey the seedling quality (including plant height, number of roots, root length, and fresh weight of 100 plants).

[0178] 3) Investigation of prevention efficacy in the rice seedling field: The results of the seedling bed test were investigated before rice transplanting on July 13, 2019. Samples were taken at 5 points in each plot, and 100 plants were surveyed at each point. The number of diseased plants in each plant and the total number of plants were investigated.

[0179] 3) Field control efficacy survey: The field test results were investigated during the rice heading stage on September 2, 2019. Samples were taken at 5 points in each plot, with 20 clusters at each point. The number of diseased plants in each plant and the total number of plants were investigated.

[0180] From rice sowing and germination to harvest, the field growth conditions of rice were observed at irregular intervals. No obvious symptoms of pesticide damage were found, and the test agents had no effect on non-target organisms.

[0181] Calculation method of drug efficacy:

[0182]

[0183]

[0184]

[0185] The results of the field efficacy test are shown in the table below:

[0186] Table 5 Control effect of the tested agents on rice seedling disease

[0187]

[0188]

[0189] As can be seen from Table 5, the three-way mixed pesticide showed obvious control effects on rice seedling disease. Compared with the blank control pesticide treatment, the seedling emergence rate increased and the diseased plant rate decreased, showing a good control effect.

[0190] Table 6 Test results of the effects of the tested pesticides on the quality of rice seedlings

[0191]

[0192] As can be seen from the test results of rice seedling quality in Table 6, the three-component mixed agent of the present invention has basically the same effect on the indicators such as plant height, root length, number of roots, and fresh weight of rice seedlings as the blank control, indicating that the rice seeds will not be damaged by the phytotoxicity after being treated with the seed coating of each formulation.

[0193] Example 4: Field efficacy test on rice thrips

[0194] Test basis: The test refers to GB / T 17980.77-2004 "Guidelines for Field Efficacy Tests of Pesticides (II) Part 77: Insecticides for Control of Rice Thrips".

[0195] Target of prevention and control: Rice thrips (Chloethrips oryzae).

[0196] Experimental crops and growth conditions: Rice (Huangkexiang No. 3).

[0197] The test was conducted at the experimental base of the Academy of Agricultural Sciences in Huanggang City, Hubei Province. The soil was medium loam with a pH of 7.0 and an organic matter content of 1.6%, indicating moderate fertility. The experimental site is located in an area prone to rice thrips infestation. The experimental fields were well-irrigated, and cultivation conditions (soil type, water and fertilizer management, planting density, and growing period) were uniform across the experimental plots. Rice was planted on all four sides of the experimental plots, following the previous crop, which was idle during the winter.

[0198] Dosage of drugs: See the table below for the test drugs and dosage:

[0199] Table 7 Test agents and dosage

[0200]

[0201] Plot arrangement: The plot treatments of test agent, control agent and blank control were arranged in random blocks.

[0202] Plot area and repetition: Plot area 10m 2 , repeated 4 times, small ridges were built between the plots, single row and single irrigation were carried out, and protection rows were set up around the experimental field.

[0203] Application method: Dress the rice seeds before sowing. On May 9, 2019, conventional dilution was used for seed dressing. The diluted agent was evenly mixed with the seeds according to the drug-to-seed ratio, ensuring that the solution fully contacted the rice seeds.

[0204] Sowing: Sowing and seedling raising will be carried out on May 10, 2019.

[0205] Experimental survey: The experiment was surveyed twice. The first survey was conducted 14 days after sowing when rice thrips were sporadically infesting the area. The number of curled tips and the total number of leaves were surveyed. The second survey was conducted 25 days after sowing. Samples were taken at 5 points in each plot, and each survey point was 25 cm wide. 2 ×25cm 2 , investigate and record the number of curl tips and total number of leaves.

[0206] From the time rice seedlings emerged to before harvest, the field growth conditions of rice were observed at irregular intervals, and no obvious symptoms of pesticide damage were found.

[0207] Calculation method of drug efficacy:

[0208]

[0209]

[0210] The test results are shown in the table below:

[0211] Table 8 Control effect of the tested agents on rice thrips

[0212]

[0213]

[0214] As can be seen from Table 8, the three-component mixed agent of the present invention shows obvious control effect on rice thrips disease. Compared with the blank control agent treatment group, the curling rate is reduced, and it has a good control effect and can effectively control the damage of rice thrips.

[0215] In laboratory toxicity assays and field efficacy tests, the prothioconazole compounded with trifluopram and thiamethoxam demonstrated significant control efficacy against rice bakanae disease and rice thrips. The resulting pesticide composition or formulation exhibited significant control efficacy, outperforming single agents in delaying the development of resistance and prolonging drug retention, while also being safe for crop seeds. Furthermore, no effects of the compounded formulation on crops or other non-target organisms were observed in the tests, demonstrating that the resulting pesticide composition or formulation, while enhancing synergistic bactericidal and insecticidal efficacy, can reduce production and use costs and maintain crop safety.

[0216] Although the present invention has been described in detail above using general descriptions and specific implementation plans, it is obvious to those skilled in the art that some modifications or improvements can be made thereto based on the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention shall fall within the scope of protection claimed by the present invention.

Claims

1. A pesticide composition containing prothioconazole, characterized in that: The active ingredients of the pesticide composition consist of a fungicide and an insecticide, the fungicide includes prothioconazole and fluopicolide, the insecticide is thiamethoxam, the mass ratio of the fungicide prothioconazole to fluopicolide is 1:8-8:1, and the mass ratio of the fungicide to the insecticide is 2:5-2:

45.

2. The pesticide composition according to claim 1, characterized in that The mass ratio of the fungicide prothioconazole to pentoxafen is 1:8-4:

1.

3. The pesticide composition according to claim 1, characterized in that Based on the total weight of the pesticide composition as 100wt%, the total content of the fungicide in the pesticide composition is 0.5% to 50%.

4. The pesticide composition according to claim 3, characterized in that Based on the total weight of the pesticide composition as 100wt%, the total content of the fungicide in the pesticide composition is 2% to 20%.

5. The pesticide composition according to claim 1, characterized in that Based on the total weight of the pesticide composition as 100wt%, the content of the insecticide in the pesticide composition is 1% to 80%.

6. The pesticide composition according to claim 5, characterized in that Based on the total weight of the pesticide composition as 100wt%, the content of the insecticide in the pesticide composition is 2% to 60%.

7. The pesticide composition according to claim 1, characterized in that In addition to the active ingredients, the pesticide composition also includes auxiliary ingredients, which are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoaming agents, solvents, preservatives, stabilizers, warning colors, film-forming agents, synergists and carriers.

8. The pesticide composition according to claim 1, characterized in that The pesticide composition can be prepared into any formulation form permitted in agriculture; the formulation form is any one of seed treatment dry powder, seed treatment dispersible powder, seed treatment liquid, seed treatment emulsion or seed treatment suspension.

9. The pesticide composition according to claim 8, characterized in that The preparation dosage form is a seed treatment suspension.

10. Use of the pesticide composition according to any one of claims 1 to 9 for preventing and controlling plant diseases and / or insect pests, characterized in that: The plant is rice, the rice disease is rice bakanae, and the rice insect pest is rice thrips.

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