A deep-sea organism cultivation test device and test method

Abstract

A deep-sea organism culture test device and test method, comprising a support, a culture chamber supported and installed on the top surface of which, a feeding port is provided at the top position of the support, a feeding port ball valve is installed in series at the feeding port through a flange, a sewage outlet is provided at the bottom position of the culture chamber, and a sewage outlet ball valve is installed in series at the sewage outlet through a flange; a biological transfer port and a pressure adding and unloading port are respectively provided on both sides of the culture chamber, a biological transfer switch valve is installed at the biological transfer port, the biological transfer switch valve is connected to a deep-sea fidelity sampling device through a flange, and the biological transfer switch valve is opened to transfer deep-sea organisms into the culture chamber, a pressure adding and unloading pipeline is installed at the pressure adding and unloading port, and the culture chamber is pressurized and unpressurized from the pressure adding and unloading port through the pressure adding and unloading pipeline to simulate the high-pressure environment of the deep sea; a sampling port is also provided obliquely above the culture chamber, and the sampling port is sealed by a sampling port screw plug, which greatly improves the convenience of experimental research and reduces scientific research costs.

Description

Technical Field

[0001] The present invention relates to the technical field of deep-sea organism cultivation test devices, in particular to a deep-sea organism cultivation test device and a test method. Background Art

[0002] At present, the research on deep-sea organisms mainly adopts deep-sea in situ observation and laboratory analysis after sampling. It is necessary to dispatch manned or unmanned deep-sea submersibles to the deep sea for long-term and high-frequency observation operations. The operation cost is high. In addition, due to the special high-pressure environment of the deep sea, deep-sea organisms cannot survive after being brought back to the laboratory, which greatly limits laboratory research work. Summary of the Invention

[0003] In response to the shortcomings of the above-mentioned existing production technologies, the applicant provides a deep-sea organism cultivation test device and test method, which can conveniently realize the cultivation of deep-sea organisms in a laboratory fidelity environment, greatly improving the convenience of experimental research and reducing scientific research costs.

[0004] The technical solutions adopted in the present invention are as follows:

[0005] A deep-sea organism culture test device comprises a support, a culture chamber is supported and installed on the top surface of the support, a feeding port is provided at the top position of the culture chamber, a No. 1 feeding port ball valve and a No. 2 feeding port ball valve are installed in series at the feeding port through a flange, a sewage outlet is provided at the bottom position of the culture chamber, a No. 1 sewage outlet ball valve and a No. 2 sewage outlet ball valve are installed in series at the sewage outlet through a flange; a biological transfer port and a pressure adding and unloading port are respectively provided on both sides of the culture chamber, a biological transfer switch valve is installed at the biological transfer port, the biological transfer switch valve is connected to a deep-sea fidelity sampling device through a flange, the biological transfer switch valve is opened to transfer deep-sea organisms into the culture chamber, a pressure adding and unloading pipeline is installed at the pressure adding and unloading port, the culture chamber is pressurized and unpressurized from the pressure adding and unloading pipeline, so as to simulate the high-pressure environment of the deep sea; a sampling port is also provided obliquely above the culture chamber, and the sampling port is sealed by a sampling port screw plug.

[0006] Its further technical solution is:

[0007] The culture chamber adopts a spherical structure.

[0008] The No. 1 feeding port ball valve, the No. 2 feeding port ball valve, the No. 1 sewage outlet ball valve and the No. 2 sewage outlet ball valve are all manual ball valves.

[0009] The support is an integrated structure.

[0010] The cross section of the support is a truncated cone structure.

[0011] The culture cabin and the support are welded into one body.

[0012] The interior of the support is provided with a hollow structure, and a plurality of rectangular openings are provided around the support, and each rectangular opening corresponds to the No. 1 sewage outlet ball valve and the No. 2 sewage outlet ball valve.

[0013] A test method for a deep-sea organism cultivation test device comprises the following steps:

[0014] Step 1: Inject seawater, close the No. 1 sewage outlet ball valve and the biological transfer switch valve, open the No. 1 feeding port ball valve and the No. 2 feeding port ball valve, remove the sampling port plug, and fill the culture chamber with seawater from the sampling port;

[0015] Step 2: Apply pressure, close the No. 2 feeding port ball valve and the biological transfer switch valve, install the sampling port plug, and use the pressurizing equipment to pressurize the culture chamber to the target pressure through the pressure increase and relief pipeline;

[0016] Step 3: Transporting deep-sea organisms: Open the organism transfer valve and drive the deep-sea organisms into the culture chamber through temperature or vibration.

[0017] Step 4: Close the biological transfer switch valve, remove the deep-sea fidelity sampling device, and complete the fidelity transfer operation of deep-sea organisms;

[0018] Step 5: Keep the ball valve of the No. 2 feeding port closed, open the ball valve of the No. 1 feeding port, and put the biological feed between the ball valves of the No. 1 feeding port and the No. 2 feeding port; then close the ball valve of the No. 1 feeding port, slowly open the ball valve of the No. 2 feeding port, and at the same time use the pressure increasing equipment to pressurize the culture chamber through the pressure increasing and reducing pipelines to keep the pressure in the culture chamber constant; after the ball valve of the No. 2 feeding port is fully opened, stop pressurizing, and the biological feed will naturally fall into the culture chamber under the action of its own gravity, then close the ball valve of the No. 2 feeding port, open the ball valve of the No. 1 feeding port, and complete the feeding operation;

[0019] Step 6: Repeat the fifth step for multiple feeding operations to ensure that the organisms in the cabin can come into contact with the biological feed;

[0020] Step 7: When discharging the biological excrement and biological feed residue in the culture chamber, keep the No. 1 sewage outlet ball valve closed and close the No. 2 sewage outlet ball valve; slowly open the No. 1 sewage outlet ball valve, and at the same time use the pressure-increasing equipment to pressurize the culture chamber through the pressure-increasing and unloading pipelines to keep the pressure in the culture chamber constant; after the No. 1 sewage outlet ball valve is fully opened, stop pressurizing, and the biological excrement and biological feed residue will naturally fall between the No. 1 sewage outlet ball valve and the No. 2 sewage outlet ball valve under the action of their own gravity, then close the No. 1 sewage outlet ball valve and open the No. 2 sewage outlet ball valve to complete the sewage discharge operation;

[0021] Step 7: After the biological culture is completed, the pressure in the culture chamber is reduced to normal pressure through the pressure-adding and pressure-reducing pipelines, the sampling port plug is removed, and the organisms are taken out of the culture chamber using the sampling equipment to carry out relevant analysis and research work.

[0022] The beneficial effects of the present invention are as follows:

[0023] The present invention has a compact and reasonable structure and is easy to operate. The feeding and sewage discharge functions are realized by opening and closing the ball valves of the feeding port and the sewage discharge port. The deep-sea organisms are transferred from the deep-sea sampling device to the culture chamber with fidelity by opening and closing the biological transfer switch valve. The pressurization and depressurization operations of the culture chamber are realized by the pressure adding and depressurizing pipelines. After the deep-sea organism cultivation test is completed, the sampling port plug is removed and the organisms can be taken out of the culture chamber.

[0024] The present invention uses an experimental device to carry out laboratory-fidelity cultivation of deep-sea organisms, which will greatly improve the convenience of experimental research and reduce scientific research costs.

[0025] The invention can be used for long-term cultivation of deep-sea organisms in a laboratory simulated environment. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 It is a structural schematic diagram of the present invention.

[0027] Figure 2 It is the front view of the present invention.

[0028] Figure 3 It is a schematic diagram of the internal structure of the present invention.

[0029] Figure 4 It is a top view of the present invention.

[0030] Among them: 1. Ball valve for feed port No. 1; 2. Ball valve for feed port No. 2; 3. Sampling port screw plug; 4. Pressure adding and unloading pipelines; 5. Culture chamber; 6. Ball valve for sewage outlet No. 1; 7. Ball valve for sewage outlet No. 2; 8. Support; 9. Biological transfer switch valve. DETAILED DESCRIPTION

[0031] The specific embodiments of the present invention will be described below with reference to the accompanying drawings.

[0032] like Figure 1-Figure 4As shown, the deep-sea biological culture test device of this embodiment includes a support 8, the top surface of the support 8 supports and installs a culture chamber 5, the top position of the culture chamber 5 is provided with a feeding port, and the No. 1 feeding port ball valve 1 and the No. 2 feeding port ball valve 2 are installed in series at the feeding port through a flange, and a sewage outlet is provided at the bottom position of the culture chamber 5, and the No. 1 sewage outlet ball valve 6 and the No. 2 sewage outlet ball valve 7 are installed in series at the sewage outlet through a flange; biological transfer ports and pressure adding and unloading ports are respectively provided on both sides of the culture chamber 5, and a biological transfer switch valve 9 is installed at the biological transfer port, and the biological transfer switch valve 9 is connected to the deep-sea fidelity sampling device through a flange. The deep-sea organisms are transferred into the culture chamber 5 by opening the biological transfer switch valve 9, and a pressure adding and unloading pipeline 4 is installed at the pressure adding and unloading port. The culture chamber 5 is pressurized and unloaded from the pressure adding and unloading port through the pressure adding and unloading pipeline 4 to simulate the high-pressure environment of the deep sea; a sampling port is also provided obliquely above the culture chamber 5, and the sampling port is closed by a sampling port screw plug 3.

[0033] The culture chamber 5 adopts a spherical structure.

[0034] The No. 1 feeding port ball valve 1, the No. 2 feeding port ball valve 2, the No. 1 sewage outlet ball valve 6 and the No. 2 sewage outlet ball valve 7 are all manual ball valves.

[0035] The support 8 is an integrated structure.

[0036] The cross section of the support 8 is a truncated cone structure.

[0037] The culture chamber 5 and the support 8 are welded into one body.

[0038] The interior of the support 8 is provided with a hollow structure, and multiple rectangular openings are provided around the support 8, each rectangular opening corresponding to the No. 1 sewage outlet ball valve 6 and the No. 2 sewage outlet ball valve 7.

[0039] The present invention is based on the analysis of the current status of deep-sea organism research and can realize the long-term cultivation of deep-sea organisms in a laboratory simulated environment.

[0040] The specific structure and functions of the deep-sea organism cultivation test device of the present invention are as follows:

[0041] It mainly includes a culture chamber 5, a biological transfer switch valve 9, two feeding port ball valves connected in series, two sewage outlet ball valves connected in series, a sampling port screw plug 3 and a pressure adding and unloading pipeline 4.

[0042] A feeding port is provided on the top of the culture chamber 5. The No. 1 feeding port ball valve 1 and the No. 2 feeding port ball valve 2 are connected in series at the feeding port position through flanges. By opening and closing the two feeding port ball valves in sequence, the feeding operation in a high-pressure environment is achieved.

[0043] A sewage outlet is provided at the bottom of the culture chamber 5, and the No. 1 sewage outlet ball valve 6 and the No. 2 sewage outlet ball valve 7 are connected in series at the sewage outlet position through flanges; by opening and closing the two sewage outlet ball valves in sequence, sewage discharge operations in a high-pressure environment are achieved.

[0044] Pressure adding and unloading ports, sampling ports and biological transfer ports are respectively provided at other positions of the culture chamber 5; the biological transfer switch valve 9 is connected to the biological transfer port, and after the deep-sea fidelity sampling device (not shown in the figure) is docked with the outer flange of the biological transfer switch valve 9, the biological transfer switch valve 9 can be opened to transfer the deep-sea organisms to the culture chamber 5; the culture chamber 5 is pressurized and unloaded from the pressure adding and unloading ports through the pressure adding and unloading pipeline 4 to simulate the deep-sea high-pressure environment; during the biological culture process, the sampling port is closed with the sampling port plug 3, and after the biological culture is completed and the pressure is released, the sampling port plug 3 is opened to carry out the sampling operation.

[0045] In actual work process:

[0046] First, close the No. 1 sewage outlet ball valve 6 and the biological transfer switch valve 9, open the No. 1 feeding port ball valve 1 and the No. 2 feeding port ball valve 2, remove the sampling port plug 3, and fill the culture chamber 5 with seawater from the sampling port.

[0047] Then, close the No. 2 feeding port ball valve 2 and the biological transfer switch valve 9, install the sampling port plug 3, and use the pressurizing equipment to pressurize the culture chamber 5 to the target pressure through the pressure increase and release pipeline 4.

[0048] Then, the deep-sea fidelity sampling device (not shown) is connected to the outer flange of the biological transfer switch valve 9, the biological transfer switch valve 9 is opened, and the deep-sea organisms are driven into the culture chamber 5 by means of temperature or vibration.

[0049] Then, close the biological transfer switch valve 9, remove the deep-sea fidelity sampling device (not shown in the figure), and complete the fidelity transfer operation of the deep-sea organisms.

[0050] Subsequently, keep the No. 2 feeding port ball valve 2 in the closed state, open the No. 1 feeding port ball valve 1, and put the biological feed between the No. 1 feeding port ball valve 1 and the No. 2 feeding port ball valve 2; close the No. 1 feeding port ball valve 1, slowly open the No. 2 feeding port ball valve 2, and at the same time use the pressurizing equipment to pressurize the culture cabin 5 through the pressure increase and reduction pipeline 4 to keep the pressure in the culture cabin 5 unchanged; after the No. 2 feeding port ball valve 2 is fully opened, stop pressurizing, and the biological feed naturally falls into the culture cabin 5 under the action of its own gravity, and then close the No. 2 feeding port ball valve 2, open the No. 1 feeding port ball valve 1, and complete the feeding operation; because some biological feed will fall into the sewage outlet, in order to ensure that the organisms in the cabin can come into contact with the biological feed, this feeding operation can be repeated multiple times.

[0051] When it is necessary to discharge the biological excrement and biological feed residue in the culture chamber 5, keep the No. 1 sewage outlet ball valve 6 in a closed state and close the No. 2 sewage outlet ball valve 7; slowly open the No. 1 sewage outlet ball valve 6, and at the same time use the pressurizing equipment to pressurize the culture chamber 5 through the pressure adding and unloading pipeline 4 to keep the pressure in the culture chamber 5 unchanged; after the No. 1 sewage outlet ball valve 6 is fully opened, stop pressurizing, and the biological excrement and biological feed residue naturally fall between the No. 1 sewage outlet ball valve 6 and the No. 2 sewage outlet ball valve 7 under the action of their own gravity, then close the No. 1 sewage outlet ball valve 6, open the No. 2 sewage outlet ball valve 7, and complete the sewage discharge operation.

[0052] Finally, after the biological culture is completed, the pressure in the culture chamber 5 is reduced to normal pressure through the pressure increase and reduction pipeline 4, the sampling port plug 3 is removed, and the organisms are taken out of the culture chamber 5 using a sampling device to carry out relevant analysis and research work.

[0053] The above description is an explanation of the present invention, not a limitation of the present invention. The scope of the present invention is defined in the claims. Any modifications may be made within the scope of protection of the present invention.

Claims

1. A deep-sea organism cultivation test device, characterized in that: The invention comprises a support (8), the top surface of the support (8) supports and installs a culture cabin (5), the top position of the culture cabin (5) is provided with a feeding port, a No. 1 feeding port ball valve (1) and a No. 2 feeding port ball valve (2) are installed in series at the feeding port through a flange, a sewage outlet is provided at the bottom position of the culture cabin (5), a No. 1 sewage outlet ball valve (6) and a No. 2 sewage outlet ball valve (7) are installed in series at the sewage outlet through a flange; a biological transfer port and a pressure relief port are respectively provided on both sides of the culture cabin (5), and a biological transfer port and a pressure relief port are installed on both sides of the culture cabin (5). A biological transfer switch valve (9) is installed at the biological transfer port, and the biological transfer switch valve (9) is connected to the deep-sea fidelity sampling device through a flange. The biological transfer switch valve (9) is opened to transfer the deep-sea organisms into the culture chamber (5). A pressure-adding and unloading pipe (4) is installed at the pressure-adding and unloading port, and the culture chamber (5) is pressurized and unpressurized from the pressure-adding and unloading port through the pressure-adding and unloading pipe (4) to simulate the deep-sea high-pressure environment. A sampling port is also provided obliquely above the culture chamber (5), and the sampling port is sealed by a sampling port screw plug (3); When feeding: keep the No. 2 feeding port ball valve (2) in a closed state, open the No. 1 feeding port ball valve (1), and put the biological feed between the No. 1 feeding port ball valve (1) and the No. 2 feeding port ball valve (2); then close the No. 1 feeding port ball valve (1), slowly open the No. 2 feeding port ball valve (2), and at the same time use a pressurizing device to pressurize the culture chamber (5) through the pressure-increasing and unloading pipeline (4) to keep the pressure in the culture chamber (5) constant; after the No. 2 feeding port ball valve (2) is fully opened, stop pressurizing, and the biological feed naturally falls into the culture chamber (5) under the action of its own gravity, then close the No. 2 feeding port ball valve (2), open the No. 1 feeding port ball valve (1), and complete the feeding operation; When discharging biological excrement and biological feed residues: keep the No. 1 sewage outlet ball valve (6) in a closed state and close the No. 2 sewage outlet ball valve (7); slowly open the No. 1 sewage outlet ball valve (6) and at the same time use the pressure-increasing device to pressurize the culture chamber (5) through the pressure-increasing and unloading pipeline (4) so ​​that the pressure in the culture chamber (5) remains unchanged; after the No. 1 sewage outlet ball valve (6) is fully opened, stop pressurizing, and the biological excrement and biological feed residues naturally fall between the No. 1 sewage outlet ball valve (6) and the No. 2 sewage outlet ball valve (7) under the action of their own gravity, then close the No. 1 sewage outlet ball valve (6) and open the No. 2 sewage outlet ball valve (7) to complete the sewage discharge operation.

2. A deep-sea organism cultivation test device according to claim 1, characterized in that: The culture chamber (5) adopts a spherical structure.

3. The deep-sea organism cultivation test device according to claim 1, characterized in that: The No. 1 feeding port ball valve (1), the No. 2 feeding port ball valve (2), the No. 1 sewage outlet ball valve (6), and the No. 2 sewage outlet ball valve (7) are all manual ball valves.

4. The deep-sea organism cultivation test device according to claim 1, characterized in that: The support (8) is an integrated structure.

5. The deep-sea organism cultivation test device according to claim 1, characterized in that: The cross section of the support (8) is a truncated cone structure.

6. The deep-sea organism cultivation test device according to claim 1, characterized in that: The culture chamber (5) and the support (8) are welded into one body.

7. The deep-sea organism cultivation test device according to claim 1, characterized in that: The support (8) is provided with a hollow structure inside, and a plurality of rectangular openings are provided around the support (8), each rectangular opening corresponding to the No. 1 sewage outlet ball valve (6) and the No. 2 sewage outlet ball valve (7).

8. A test method for the deep-sea organism cultivation test device according to claim 1, characterized in that: The steps are as follows: Step 1: inject seawater, close the No. 1 sewage outlet ball valve (6) and the biological transfer switch valve (9), open the No. 1 feeding port ball valve (1) and the No. 2 feeding port ball valve (2), remove the sampling port screw plug (3), and fill the culture chamber (5) with seawater from the sampling port; Step 2: Apply pressure, close the No. 2 feeding port ball valve (2) and the biological transfer switch valve (9), install the sampling port plug (3), and use the pressure-increasing device to pressurize the culture chamber (5) to the target pressure through the pressure-increasing and unloading pipeline (4); Step 3: Transporting deep-sea organisms: Open the organism transfer switch valve (9) and drive the deep-sea organisms into the culture chamber (5) by temperature or vibration. Step 4: Close the biological transfer switch valve (9), remove the deep-sea fidelity sampling device, and complete the fidelity transfer operation of the deep-sea organisms; Step 5: Keep the No. 2 feeding port ball valve (2) in a closed state, open the No. 1 feeding port ball valve (1), and put the biological feed between the No. 1 feeding port ball valve (1) and the No. 2 feeding port ball valve (2); then close the No. 1 feeding port ball valve (1), slowly open the No. 2 feeding port ball valve (2), and at the same time use the pressure increasing device to pressurize the culture chamber (5) through the pressure increasing and reducing pipeline (4) so ​​that the pressure in the culture chamber (5) remains unchanged; after the No. 2 feeding port ball valve (2) is fully opened, stop pressurizing, and the biological feed naturally falls into the culture chamber (5) under the action of its own gravity, then close the No. 2 feeding port ball valve (2), open the No. 1 feeding port ball valve (1), and complete the feeding operation; Step 6: Repeat the fifth step for multiple feeding operations to ensure that the organisms in the cabin can come into contact with the biological feed; Step 7: When discharging the biological excrement and biological feed residue in the culture chamber (5), keep the No. 1 sewage outlet ball valve (6) in a closed state and close the No. 2 sewage outlet ball valve (7); slowly open the No. 1 sewage outlet ball valve (6), and at the same time use the pressure-increasing device to pressurize the culture chamber (5) through the pressure-increasing and unloading pipeline (4) so ​​that the pressure in the culture chamber (5) remains unchanged; after the No. 1 sewage outlet ball valve (6) is fully opened, stop pressurizing, and the biological excrement and biological feed residue naturally fall to between the No. 1 sewage outlet ball valve (6) and the No. 2 sewage outlet ball valve (7) under the action of their own gravity, then close the No. 1 sewage outlet ball valve (6), open the No. 2 sewage outlet ball valve (7), and complete the sewage discharge operation; Step 7: After the biological culture is completed, the pressure in the culture chamber (5) is reduced to normal pressure through the pressure-adding and unloading pipeline (4), the sampling port plug (3) is removed, and the organisms are taken out of the culture chamber (5) using a sampling device to carry out relevant analysis and research work.

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