A composition for inhibiting renal sodium reabsorption, and its preparation method and application

Abstract

The present invention relates to the field of traditional Chinese medicine technology. A composition for inhibiting renal sodium reabsorption, its preparation method, and use are provided. The composition comprises the following raw materials in parts by weight: 10-12 parts of Evodia rutaecarpa, 12-16 parts of Achyranthes bidentata, 4-8 parts of Curcuma aromatica, 4-8 parts of Akebia, 8-12 parts of Glycyrrhiza uralensis, 5-7 parts of Juncus effusus, 1-3 parts of cinnamaldehyde, and 0.5-1.5 parts of folic acid. The composition is specifically designed for people with high-salt diets, has a wide variety of raw material sources, and a simple preparation process. It can overcome the limitations and toxic side effects of existing diuretics.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicines, and in particular to a composition for inhibiting renal sodium reabsorption, a preparation method and an application thereof. Background Art

[0002] Excessive salt intake can cause pathophysiological changes such as activation of the renin-angiotensin-aldosterone system, oxidative stress damage, and renal sodium and water retention. Impaired renal sodium excretion is an important factor in sodium and water retention caused by a high-salt diet. Promoting renal sodium excretion can prevent sodium and water retention. Multiple pathways that affect urinary sodium reabsorption can be specifically intervened through diuretics, but long-term, large-scale, or combined use of diuretics can lead to side effects such as metabolic disorders. Existing diuretics have the disadvantages and shortcomings of a high incidence of adverse reactions and poor tolerability, which reduces patient compliance.

[0003] Therefore, it is of great significance to explore new intervention targets and safe and effective diuretics to promote natriuresis. Summary of the Invention

[0004] The purpose of the present invention is to provide a composition for inhibiting renal sodium reabsorption, a preparation method and an application thereof, which can overcome the limitations and toxic side effects of existing diuretics.

[0005] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0006] The invention provides a composition for inhibiting sodium reabsorption in the kidney. The composition comprises the following raw materials in parts by weight: 10-12 parts of Evodia rutaecarpa, 12-16 parts of Achyranthes bidentata, 4-8 parts of Curcuma aromatica, 4-8 parts of Akebia, 8-12 parts of Licorice, 5-7 parts of Juncus effusus, 1-3 parts of cinnamaldehyde and 0.5-1.5 parts of folic acid.

[0007] Preferably, the composition comprises the following raw materials in parts by weight: 11 parts of Evodia rutaecarpa, 14 parts of Rhizoma Cynanchifoliae, 6 parts of Curcuma aromatica, 6 parts of Akebia, 10 parts of Glycyrrhiza uralensis, 6 parts of Juncus effusus, 2 parts of cinnamaldehyde, and 1 part of folic acid.

[0008] The present invention also provides a method for preparing the composition, comprising the following steps:

[0009] (1) Grinding and mixing Evodia rutaecarpa, Achyranthes bidentata, Curcuma aromatica, Akebia chinensis, Licorice root and Juncus effusus to obtain a mixture;

[0010] (2) using carbon dioxide as an extraction solvent to perform supercritical extraction on the mixed material to obtain an extract;

[0011] (3) The extract, cinnamaldehyde and folic acid are mixed to obtain the composition.

[0012] Preferably, the particle size of the crushed mixture in step (1) is 80 to 120 meshes.

[0013] Preferably, the temperature of the supercritical extraction in step (2) is 37-39° C. and the pressure is 41-43 MPa.

[0014] Preferably, the flow rate of carbon dioxide during the supercritical extraction in step (2) is 30 to 32 kg / h.

[0015] Preferably, the supercritical extraction time in step (2) is 45 to 65 minutes.

[0016] The present invention also provides application of the composition in preparing medicine for inhibiting sodium reabsorption in kidney.

[0017] The present invention also provides a medicine for inhibiting sodium reabsorption in the kidney, which contains the pharmaceutical composition.

[0018] Preferably, the medicine further contains pharmaceutically acceptable excipients.

[0019] The present invention provides a composition for inhibiting renal sodium reabsorption, as well as its preparation method and use. The composition comprises the following raw materials by weight: 10-12 parts of Evodia rutaecarpa, 12-16 parts of Achyranthes bidentata, 4-8 parts of Curcuma aromatica, 4-8 parts of Akebia, 8-12 parts of Glycyrrhiza uralensis, 5-7 parts of Juncus effusus, 1-3 parts of cinnamaldehyde, and 0.5-1.5 parts of folic acid. The composition is specifically targeted at people with high-salt diets, has a wide variety of raw material sources, and is simple to prepare. It overcomes the limitations and toxic side effects of existing diuretics. DETAILED DESCRIPTION

[0020] The invention provides a composition for inhibiting sodium reabsorption in the kidney. The composition comprises the following raw materials in parts by weight: 10-12 parts of Evodia rutaecarpa, 12-16 parts of Achyranthes bidentata, 4-8 parts of Curcuma aromatica, 4-8 parts of Akebia, 8-12 parts of Licorice, 5-7 parts of Juncus effusus, 1-3 parts of cinnamaldehyde and 0.5-1.5 parts of folic acid.

[0021] In the present invention, the composition preferably includes the following raw materials in parts by weight: 11 parts of Evodia rutaecarpa, 14 parts of Rhizoma Cyperi, 6 parts of Curcuma aromatica, 6 parts of Akebia, 10 parts of Glycyrrhiza uralensis, 6 parts of Juncus effusus, 2 parts of cinnamaldehyde, and 1 part of folic acid.

[0022] The present invention also provides a method for preparing the composition, comprising the following steps:

[0023] (1) Grinding and mixing Evodia rutaecarpa, Achyranthes bidentata, Curcuma aromatica, Akebia chinensis, Licorice root and Juncus effusus to obtain a mixture;

[0024] (2) using carbon dioxide as an extraction solvent to perform supercritical extraction on the mixed material to obtain an extract;

[0025] (3) The extract, cinnamaldehyde and folic acid are mixed to obtain the composition.

[0026] In the present invention, the particle size of the pulverized mixture in step (1) is preferably 80 to 120 meshes, more preferably 100 meshes.

[0027] In the present invention, the temperature of the supercritical extraction in step (2) is preferably 37-39° C., more preferably 38° C., and the pressure is preferably 41-43 MPa, more preferably 42 MPa.

[0028] In the present invention, the flow rate of carbon dioxide during the supercritical extraction in step (2) is preferably 30 to 32 kg / h, more preferably 31 kg / h.

[0029] In the present invention, the time of supercritical extraction in step (2) is preferably 45 to 65 minutes, more preferably 55 minutes.

[0030] The present invention also provides application of the composition in preparing medicine for inhibiting sodium reabsorption in kidney.

[0031] The present invention also provides a medicine for inhibiting sodium reabsorption in the kidney, which contains the pharmaceutical composition.

[0032] In the present invention, the drug preferably further contains pharmaceutically acceptable excipients.

[0033] The technical solutions provided by the present invention are described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0034] Example 1

[0035] A method for preparing a composition for inhibiting renal sodium reabsorption comprises the following steps:

[0036] (1) Weigh the raw materials according to the mass ratio of 10 parts of Evodia rutaecarpa, 16 parts of Rhizoma Cynanchifoliae, 4 parts of Curcuma aromatica, 4 parts of Akebia, 12 parts of Glycyrrhiza uralensis, 5 parts of Juncus effusus, 3 parts of cinnamaldehyde, and 0.5 parts of folic acid;

[0037] (2) Grinding Evodia rutaecarpa, Achyranthes bidentata, Curcuma aromatica, Akebia chinensis, Licorice root and Juncus effusus into 80 meshes and mixing them to obtain a mixture;

[0038] (3) using carbon dioxide as an extraction solvent, supercritical extraction was performed on the mixture at 39°C and 41 MPa for 65 min, with the flow rate of carbon dioxide during supercritical extraction set to 30 kg / h to obtain an extract;

[0039] (4) The extract, cinnamaldehyde and folic acid are mixed to obtain the composition.

[0040] Example 2

[0041] A method for preparing a composition for inhibiting renal sodium reabsorption comprises the following steps:

[0042] (1) Weigh the raw materials according to the mass ratio of 12 parts of Evodia rutaecarpa, 12 parts of Rhizoma Cynanchifoliae, 8 parts of Curcuma aromatica, 8 parts of Akebia, 8 parts of Glycyrrhiza uralensis, 7 parts of Juncus effusus, 1 part of cinnamaldehyde, and 1.5 parts of folic acid;

[0043] (2) Grinding Evodia rutaecarpa, Achyranthes bidentata, Curcuma aromatica, Akebia caulis, Licorice root and Juncus effusus into 120 meshes and mixing them to obtain a mixture;

[0044] (3) using carbon dioxide as an extraction solvent, subjecting the mixed material to supercritical extraction at 37° C. and 43 MPa for 45 min, with the carbon dioxide flow rate during supercritical extraction set to 32 kg / h, to obtain an extract;

[0045] (4) The extract, cinnamaldehyde and folic acid are mixed to obtain the composition.

[0046] Example 3

[0047] A method for preparing a composition for inhibiting renal sodium reabsorption comprises the following steps:

[0048] (1) Weigh the raw materials according to the mass ratio of 11 parts of Evodia rutaecarpa, 14 parts of Rhizoma Cynanchifoliae, 6 parts of Curcuma aromatica, 6 parts of Akebia, 10 parts of Glycyrrhiza uralensis, 6 parts of Juncus effusus, 2 parts of cinnamaldehyde, and 1.0 part of folic acid;

[0049] (2) Grinding Evodia rutaecarpa, Achyranthes bidentata, Curcuma aromatica, Akebia trifoliata, Licorice root, and Juncus effusus into 100 mesh sizes and mixing them to obtain a mixture;

[0050] (3) using carbon dioxide as an extraction solvent, supercritical extraction was performed on the mixture at 38°C and 42 MPa for 55 min, with the flow rate of carbon dioxide during supercritical extraction set to 31 kg / h to obtain an extract;

[0051] (4) The extract, cinnamaldehyde and folic acid are mixed to obtain the composition.

[0052] Test Example: Inhibition of Renal Sodium Reabsorption Test

[0053] (1) Experimental subjects: 60 salt-sensitive rats weighing 210-220 g were randomly divided into five groups, with 12 rats in each group, numbered as experimental groups 1-3 and control groups 1-2.

[0054] (2) Sample preparation: The composition prepared in Example 1 was dissolved in purified water to prepare a 0.015 g / mL solution, which was numbered as Sample 1; the composition prepared in Example 2 was dissolved in purified water to prepare a 0.015 g / mL solution, which was numbered as Sample 2; the composition prepared in Example 3 was dissolved in purified water to prepare a 0.015 g / mL solution, which was numbered as Sample 3.

[0055] (3) Experimental design: Each rat in experimental group 1 was gavaged with sample 1 at a standard of 20 mL / kg; each rat in experimental group 2 was gavaged with sample 2 at a standard of 20 mL / kg; each rat in experimental group 3 was gavaged with sample 3 at a standard of 20 mL / kg; rats in control groups 1 and 2 were gavaged with normal saline at a standard of 20 mL / kg.

[0056] During the experiment, rats in experimental groups 1-3 and control groups 1-2 were fed the same diet. The rats in experimental groups 1-3 and control group 1 were provided with 8% saline solution for drinking, while the rats in control group 2 were provided with purified water. The experiment lasted for 7 days, and afterward, the blood pressure and urine sodium levels of the rats in each group were measured.

[0057] (4) Test results: The blood pressure of rats in test groups 1 to 3 and control group 2 were normal, with no significant difference, and no symptoms of hypertension; however, the blood pressure of rats in control group 1 was significantly elevated, higher than that of rats in control group 2, and also higher than that of rats showing symptoms of hypertension. The average sodium content in the urine of rats in test group 1 was 6.71 μmol / 24h / g BW, the average sodium content in the urine of rats in test group 2 was 6.33 μmol / 24h / g BW, the average sodium content in the urine of rats in test group 3 was 659 μmol / 24h / g BW, the average sodium content in the urine of rats in control group 1 was 3.17 μmol / 24h / g BW, and the average sodium content in the urine of rats in control group 2 was 3.20 μmol / 24h / g BW. This shows that the composition prepared by the present invention can effectively inhibit renal sodium reabsorption and reduce the problem of high blood pressure caused by a high-salt diet.

[0058] As can be seen from the above examples, the present invention provides a composition for inhibiting renal sodium reabsorption, as well as its preparation method and use. The composition comprises the following raw materials by weight: 10-12 parts of Evodia rutaecarpa, 12-16 parts of Achyranthes bidentata, 4-8 parts of Curcuma aromatica, 4-8 parts of Akebia, 8-12 parts of Glycyrrhiza uralensis, 5-7 parts of Juncus effusus, 1-3 parts of cinnamaldehyde, and 0.5-1.5 parts of folic acid. This composition is specifically targeted at people with high-salt diets, utilizes a wide variety of raw materials, and is simple to prepare. It overcomes the limitations and toxic side effects of existing diuretics.

[0059] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as within the scope of protection of the present invention.

Claims

1. A composition for inhibiting renal sodium reabsorption, characterized in that: The composition is prepared from the following raw materials in parts by weight: 10-12 parts of Evodia rutaecarpa, 12-16 parts of Rhizoma Cynanchifoliae, 4-8 parts of Curcuma aromatica, 4-8 parts of Akebia, 8-12 parts of Glycyrrhiza uralensis, 5-7 parts of Juncus effusus, 1-3 parts of cinnamaldehyde, and 0.5-1.5 parts of folic acid; The preparation method of the composition comprises the following steps: (1) Grinding and mixing Evodia rutaecarpa, Achyranthes bidentata, Curcuma aromatica, Akebia trifoliata, Licorice root and Juncus effusus to obtain a mixture; (2) Using carbon dioxide as an extraction solvent, the mixed material is subjected to supercritical extraction to obtain an extract; (3) The extract, cinnamaldehyde and folic acid are mixed to obtain the composition.

2. The composition according to claim 1, characterized in that The composition is prepared from the following raw materials in parts by weight: 11 parts of Evodia rutaecarpa, 14 parts of Rhizoma Cynanchifoliae, 6 parts of Curcuma aromatica, 6 parts of Akebia, 10 parts of Glycyrrhiza uralensis, 6 parts of Juncus effusus, 2 parts of cinnamaldehyde and 1 part of folic acid.

3. The method for preparing the composition according to claim 1 or 2, characterized in that: The steps include: (1) Grinding and mixing Evodia rutaecarpa, Achyranthes bidentata, Curcuma aromatica, Akebia trifoliata, Licorice root and Juncus effusus to obtain a mixture; (2) Using carbon dioxide as an extraction solvent, the mixed material is subjected to supercritical extraction to obtain an extract; (3) The extract, cinnamaldehyde and folic acid are mixed to obtain the composition.

4. The preparation method according to claim 3, characterized in that The particle size of the mixture after pulverization in step (1) is 80-120 mesh.

5. The preparation method according to claim 4, characterized in that The temperature of the supercritical extraction in step (2) is 37-39° C. and the pressure is 41-43 MPa.

6. The preparation method according to claim 5, characterized in that The flow rate of carbon dioxide during the supercritical extraction in step (2) is 30-32 kg / h.

7. The preparation method according to any one of claims 3 to 6, characterized in that The supercritical extraction time in step (2) is 45 to 65 minutes.

8. Use of the composition according to claim 1 or 2 in the preparation of a medicament for inhibiting renal sodium reabsorption.

9. A drug for inhibiting renal sodium reabsorption, characterized in that: The medicine consists of the pharmaceutical composition according to claim 1 or 2 and pharmaceutically acceptable excipients.