A fermented facial mask liquid and its preparation method and use

Abstract

The present invention belongs to the field of daily chemical new materials, and discloses a method for preparing a fermented facial mask liquid, wherein fermented bacteria are inoculated into a fermentation culture medium for fermentation treatment to obtain a fermented facial mask liquid; the fermentation culture medium contains a carbon source, a tremella extract, a yeast extract, rice, and wheat germ; the fermented bacteria are a 10-bacteria type bifidobacterium probiotic powder household lactic acid bacteria starter provided by Angel Yeast Co., Ltd. The method uses tremella extract, yeast extract, wheat germ, and rice for mixed fermentation, and the obtained fermentation product has a viscosity of more than 290 mPa·s. After dilution, the adjustable viscosity range is 100 to 300 mPa·s, and it still has the skin care functions of firming, soothing, and anti-wrinkle under low concentration conditions, and has good safety, mildness, and non-irritation. At the same time, the present invention also provides a fermented facial mask liquid and its application.

Description

Technical Field

[0001] The invention relates to the field of new daily chemical materials, and in particular to a fermented facial mask liquid and a preparation method and application thereof. Background Art

[0002] CN117860647A discloses a facial mask liquid composition with multiple functions, a preparation method and application thereof, comprising 1-10% of a skin conditioner and 90-99% of a facial mask liquid auxiliary material matrix; wherein the skin conditioner comprises allantoin, panthenol, a plant extract and a biological fermentation product; the plant extract comprises a Tremella fuciformis fruiting body extract, a bergamot leaf extract, a magnolia flower extract, a white water lily extract, a saffron extract, a complex of a spinosa fruit extract / a longicorn Kappaphycus extract, an ENNACOMPLEX 262 compound sensitizer, a complex of a kudzu root extract / anemarrhena root extract / acacia bud extract and a liquorice root extract; the biological fermentation product comprises a lactobacillus / soy milk fermentation product filtrate, a lactobacillus / rye powder fermentation product, a lactobacillus / granadilla fermentation product extract and a lactobacillus / algae extract fermentation product.

[0003] CN117771151A discloses a silica water repairing facial mask and a preparation method thereof, comprising the following ingredients in mass ratio: 5-20 parts of silica water, 10-20 parts of moisturizer, 0.2-0.5 parts of thickener, 0.05-0.3 parts of hyaluronic acid, 0.01-1 parts of repairing ingredients, 20-30 parts of deionized water, 3-5 parts of konjac slurry, 0.1-0.3 parts of vitamin E, 0.01-0.03 parts of quinoa extract, and 0.01-0.06 parts of rice fermentation product filtrate.

[0004] CN113576985B discloses a highly permeable water-locking composition and a soothing and moisturizing facial mask liquid and a preparation method thereof, including composition A, composition B, composition C, a soothing agent, a repairing agent, and a moisturizing agent. The facial mask liquid includes a highly permeable water-locking composition, glycerin, propylene glycol, trehalose, sodium hyaluronate, a thickener, disodium EDTA, arginine, p-hydroxyacetophenone, 1,2-hexanediol, and water. The preparation method of the facial mask liquid is as follows: first prepare a highly permeable water-locking composition; mix and heat glycerin, a portion of propylene glycol, trehalose, sodium hyaluronate, a thickener, a chelating agent, and water, stir evenly to obtain a base liquid; cool down and add arginine to stir evenly; mix and heat the remaining propylene glycol, p-hydroxyacetophenone, and 1,2-hexanediol, stir, and then add them to the base liquid to stir; cool down and add the highly permeable water-locking composition, stir evenly to obtain a facial mask liquid.

[0005] It can be learned from the above literature that existing facial mask liquids are basically compounded and need to add thickeners to adjust the viscosity of the facial mask liquid.

[0006] Customers now demand a facial mask liquid that can be directly applied without complicated preparation and has certain firming, soothing, anti-wrinkle and other effects. This poses a challenge to product development. It not only meets the needs of direct use, but also needs to meet the needs of different customers for flexible viscosity preparation. This also means that in the case of sufficient dilution, the functional effect should be retained and the effect should be more obvious. Therefore, comprehensive development of the formula is imperative. Summary of the invention

[0007] The object of the present invention is to provide a method for preparing a fermented facial mask liquid that achieves thickening effect through microbial fermentation without the need for an emulsifier. The method uses tremella extract, yeast extract, wheat germ and rice for mixed fermentation. The obtained fermentation product has a viscosity of more than 290 mPa·s. After dilution, the adjustable viscosity range is 100 to 300 mPa·s. In addition, the product still has the skin care functions of firming, soothing and anti-wrinkle under low concentration conditions, and is safe, mild and non-irritating.

[0008] At the same time, the invention also provides a fermented facial mask liquid and an application thereof.

[0009] To achieve the above object, the present invention provides the following technical solutions: a method for preparing a fermented facial mask liquid, inoculating fermentation bacteria into a fermentation culture liquid for fermentation treatment to obtain a fermented facial mask liquid;

[0010] The fermentation culture solution contains a carbon source, a Tremella extract, a yeast extract, rice, and wheat germ;

[0011] The fermentation bacteria is a 10-bacteria type bifidobacterium probiotic powder household lactic acid bacteria starter provided by Angel Yeast Co., Ltd.

[0012] During the production process, rice and wheat germ need to be homogenized before production.

[0013] In the above-mentioned method for preparing the fermented facial mask liquid, the concentration of wheat germ in the fermentation culture liquid is 0.1wt%~5wt%, the concentration of Tremella fuciformis extract is 0.1wt%~10wt%; the concentration of rice is 0.1wt%~5wt%; and the concentration of yeast extract is 0.1wt%~10wt%.

[0014] In the above-mentioned method for preparing the fermented facial mask liquid, the carbon source is one or more of sucrose, glucose, and molasses; and the concentration of the carbon source in the fermentation culture medium is 0.1wt% to 10wt%.

[0015] In the above-mentioned method for preparing the fermented facial mask liquid, in the fermentation culture medium, the concentration of Tremella fuciformis extract is 0.2wt%~2wt%, the concentration of rice is 0.5wt%~3wt%, the concentration of wheat germ is 0.2wt%~2wt%, the concentration of yeast extract is 0.1wt%~1wt%, and the concentration of carbon source is 1wt%~3wt%.

[0016] In the above-mentioned method for preparing the fermented facial mask liquid, the added amount of the fermentation bacteria is 0.1-1 g / kg of fermentation culture liquid.

[0017] In the above-mentioned method for preparing the fermented facial mask liquid, the added amount of the fermentation bacteria is 0.3-0.6 g / kg of fermentation culture liquid.

[0018] In the above-mentioned method for preparing the fermented facial mask liquid, the method is specifically as follows:

[0019] S1, mixing a carbon source, Tremella fuciformis extract, yeast extract, wheat germ, rice and water to obtain a fermentation culture solution, and adjusting the pH of the solution;

[0020] S2, sterilizing the fermentation culture solution;

[0021] S3, adding fermentation bacteria to the cooled and sterilized fermentation culture solution, the dissolved oxygen content is 10% to 50%, the stirring speed is associated with the dissolved oxygen content, and the speed is automatically compensated and adjusted;

[0022] S4. After the fermentation culture is completed, the fermentation culture solution is sterilized;

[0023] S5, using a centrifuge to process the fermentation culture solution, and collecting the culture supernatant after centrifugation;

[0024] S6. After centrifugation, sterilize the supernatant.

[0025] In the above-mentioned method for preparing the fermented facial mask liquid, the fermentation temperature is 36 to 38° C., and the fermentation time is 6 to 72 hours;

[0026] In S1, the pH is 5.5 to 7.5.

[0027] At the same time, the present invention also discloses a fermented facial mask liquid, which is prepared by any of the above methods.

[0028] Finally, the present invention also discloses the use of the fermented facial mask liquid as described above to prepare a facial mask.

[0029] Compared with the prior art, the present invention has the following beneficial effects:

[0030] (1) The present invention selected a variety of fermentation bacteria for experiments, and confirmed that the 10-bacteria type bifidobacterium probiotic powder household lactic acid bacteria fermentation agent can achieve the dual goals of viscosity and efficacy.

[0031] Among the raw materials, wheat germ is one of the organs for wheat germination and growth, accounting for about 2.5% of the whole wheat grain. It is rich in vitamins E, B1 and protein, and has very good anti-wrinkle effects. At the same time, it contains about 47% carbohydrates, mainly starch, which is the basis for achieving the thickening effect. After the rice is hydrolyzed, it has a certain thickening ability, which can further improve the thickening effect. At the same time, the selection of fermentation bacteria is also crucial. The most preferred fermentation bacteria can not only maintain a relatively high initial thickening ability, but also keep the thickening ability of the fermentation product after dilution from dropping sharply.

[0032] (2) The present invention uses microbial fermentation to extract plants. On the one hand, the content and activity of the plant's own functional substances are extracted and retained to a high degree. On the other hand, microorganisms can produce a variety of microbial active ingredients during the metabolism of plants, while converting them into new functional ingredients and degrading plant toxicity. The present invention provides a method for preparing fermented facial mask liquids of different types of microorganisms. At the same time, the prepared fermented facial mask liquid has skin care functions of firming, soothing, and anti-wrinkle, and is safe, mild, and non-irritating. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 This is a graph showing the test results of the chicken embryo chorioallantoic membrane of the formulation of Example 1 of the present invention (5 min);

[0034] Figure 2 This is a graph showing the test results of the chicken embryo chorioallantoic membrane of the formulation of Example 1 of the present invention (0min);

[0035] Figure 3 This is a graph showing the test results of the chicken embryo chorioallantoic membrane of the formulation of Example 2 of the present invention (5 min);

[0036] Figure 4 This is a graph showing the test results of the chicken embryo chorioallantoic membrane of the formulation of Example 2 of the present invention (0min);

[0037] Figure 5 This is a graph showing the test results of the chicken embryo chorioallantoic membrane of the formulation of Example 3 of the present invention (5 min);

[0038] Figure 6 This is a graph showing the test results of the chicken embryo chorioallantoic membrane of the formulation of Example 3 of the present invention (0min);

[0039] Figure 7 This is the result of the chicken embryo chorioallantoic membrane test using normal saline (5 minutes);

[0040] Figure 8 This is the result of the chicken embryo chorioallantoic membrane test using normal saline (0min);

[0041] Fig. 9 This is the result of the chicken embryo chorioallantoic membrane test using sodium hydroxide solution (5 minutes);

[0042] Fig.10 This is the test result of chicken embryo chorioallantoic membrane using sodium hydroxide solution (0min). DETAILED DESCRIPTION

[0043] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0044] Materials and reagents

[0045] Tremella extract: Henan Longteng Bioengineering Co., Ltd., polysaccharide content 50%, 500g / bag;

[0046] Yeast extract: Guangzhou Dingguo Biotechnology Co., Ltd., 500g / bottle, brand OXOID, model LP0021B, source: imported;

[0047] Saccharomyces cerevisiae: Guangdong Microbiological Culture Collection Center, item number, CGMCC 2.1364; lyophilized powder / 1 tube;

[0048] Composite fermentation bacteria: Angel Yeast Co., Ltd., 8g / bag, Angel Diamond Yogurt Fermentation Bacteria 10-type Bifidobacterium Probiotic Powder Household Lactic Acid Bacteria Fermentation Agent 8g.

[0049] Rice: Golden Dragon Fish JD self-operated flagship store, 5kg / bag, Golden Dragon Fish’s carefully selected tribute rice.

[0050] Wheat germ: Shandong Huanfeng Longsan Food Co., Ltd., 1kg / bag, wheat germ.

[0051] Example 1

[0052] The fermentation culture solution is prepared by mixing 20g of glucose, 10g of Tremella extract, 5g of yeast extract, 5g of wheat germ and 10g of rice per 1kg, and the pH of the solution is adjusted to 7.0. After the preparation is completed, the above culture solution is sterilized at 121°C for 20min under high temperature and high pressure. After the culture solution is cooled, the composite fermentation bacteria are added to the sterilized fermentation culture solution at a ratio of 0.5g per 1kg of culture solution. The constant temperature culture temperature of the fermentation tank is set to 37±1°C, the dissolved oxygen content value is 30%, the stirring speed is related to the dissolved oxygen content, the speed is automatically compensated and adjusted, and the fermentation culture time is 48h. After the fermentation culture is completed, the culture solution is sterilized at 121°C for 20min under high temperature and high pressure. After the sterilization is completed, the fermentation culture solution is treated with a centrifuge speed of 12000rpm, and the culture supernatant after centrifugation is collected. The culture supernatant is clarified to prepare a fermented mask liquid.

[0053] Example 2

[0054] The fermentation culture solution is prepared by mixing 1g of glucose, 100g of Tremella extract, 1g of yeast extract, 50g of wheat germ and 1g of rice per 1kg, and the pH of the solution is adjusted to 7.0. After the preparation is completed, the above culture solution is sterilized at 121°C for 20min under high temperature and high pressure. After the culture solution is cooled, the composite fermentation bacteria are added to the sterilized fermentation culture solution at a ratio of 1g per 1kg of culture solution. The constant temperature culture temperature of the fermentation tank is set to 37±1°C, the dissolved oxygen content value is 50%, the stirring speed is related to the dissolved oxygen content, the speed is automatically compensated and adjusted, and the fermentation culture time is 24h. After the fermentation culture is completed, the culture solution is sterilized at 121°C for 20min under high temperature and high pressure. After the sterilization is completed, the fermentation culture solution is treated with a centrifuge speed of 12000rpm, and the culture supernatant after centrifugation is collected. The culture supernatant is clarified to prepare a fermented mask liquid.

[0055] Example 3

[0056] The fermentation culture solution is prepared by mixing 100g of glucose, 1g of Tremella extract, 100g of yeast extract, 1g of wheat germ and 50g of rice per 1kg, and the pH of the solution is adjusted to 7.0. After the preparation is completed, the above culture solution is sterilized at 121°C for 20min under high temperature and high pressure. After the culture solution is cooled, the composite fermentation bacteria are added to the sterilized fermentation culture solution at a ratio of 0.1g per 1kg of culture solution. The constant temperature culture temperature of the fermentation tank is set to 37±1°C, the dissolved oxygen content value is 20%, the stirring speed is related to the dissolved oxygen content, the speed is automatically compensated and adjusted, and the fermentation culture time is 72h. After the fermentation culture is completed, the culture solution is sterilized at 121°C for 20min under high temperature and high pressure. After the sterilization is completed, the fermentation culture solution is treated with a centrifuge speed of 12000rpm, and the culture supernatant after centrifugation is collected. The culture supernatant is clarified to prepare a fermented mask liquid.

[0057] Example 4

[0058] The same as Example 1, except that the raw material formula is as follows:

[0059] 30g glucose, 2g tremella extract, 10g yeast extract, 20g wheat germ, 5g rice, and 0.6g composite fermentation bacteria.

[0060] Example 5

[0061] The same as Example 1, except that the raw material formula is as follows:

[0062] 10g glucose, 20g tremella extract, 1g yeast extract, 2g wheat germ, 30g rice, 0.3g composite fermentation bacteria.

[0063] Comparative Example 1

[0064] The same as Example 1, except that the raw material formula is as follows:

[0065] 20g glucose, 10g tremella extract, 5g yeast extract, 15g wheat germ.

[0066] Comparative Example 2

[0067] The same as Example 1, except that the raw material formula is as follows:

[0068] 20g glucose, 10g tremella extract, 5g yeast extract, 15g rice.

[0069] Comparative Example 3

[0070] The process is substantially the same as in Example 1, except that 0.5 g of cerevisiae yeast is used as the fermentation bacteria and the fermentation temperature is 28°C.

[0071] Comparative Example 4

[0072] The process is substantially the same as in Example 2, except that 1 g of Saccharomyces cerevisiae is used as the fermentation bacteria and the fermentation temperature is 28°C.

[0073] Comparative Example 5

[0074] The process is substantially the same as in Example 3, except that 0.1 g of cerevisiae yeast is used as the fermentation bacteria and the fermentation temperature is 28°C.

[0075] Performance Testing

[0076] 1. Formula safety test

[0077] The fermented facial mask liquid obtained in Examples 1-3 was subjected to the chicken embryo chorioallantoic membrane test.

[0078] Control and test sample treatment

[0079] Sample: fermented facial mask liquid of Example 1 to Example 3.

[0080] Negative control: normal saline.

[0081] Positive control: 0.1 mol / L NaOH solution.

[0082] Test operation steps

[0083] In this test, 6 chicken embryos were selected for each group, and the conditions of the chorioallantoic membrane were recorded with a camera. A polytetrafluoroethylene resin ring was placed on the chorioallantoic membrane of the chicken embryo and photographed. The sample to be tested was added to the polytetrafluoroethylene resin ring, the time of adding the sample was recorded, and the air chamber was covered with a moistened plastic wrap. The chicken embryo was moved to a constant temperature and humidity chamber for culture, and the degree of change of each toxic effect was observed.

[0084] Calculation results:

[0085] The endpoint evaluation method was used to perform the test and calculate the endpoint evaluation (ES), with the result retained to two decimal places; the score of each chicken embryo = the sum of the bleeding, coagulation and vascular melting observed in each chicken embryo; the average of the mathematical sums obtained for ES-6 chicken embryos. The average ES score was calculated according to the following formula:

[0086]

[0087] Result judgment criteria:

[0088] ES≤4, non-irritating; 4<ES≤12, mildly irritating; 12<ES<16, moderately irritating; ES≥16, strongly irritating / corrosive.

[0089] 2. Test Results

[0090] The test results of the chicken embryo chorioallantoic membrane test are shown in Table 1.

[0091] Table 1 Test results of chicken embryo chorioallantoic membrane

[0092] project result Example 1 Non-irritating Example 2 Non-irritating Example 3 Non-irritating Positive Control Strong irritation

[0093] From Table 1 and Appendix Figures 1 to 10 It can be seen that the fermented facial mask liquids obtained in Examples 1 to 3 are non-irritating to the chicken embryo chorioallantoic membrane, which indicates that the fermented facial mask liquids obtained by fermentation in the present invention have good safety.

[0094] 2. Viscosity test

[0095] The viscosity test method is: using a rotational viscometer for testing

[0096] Select the appropriate rotor and speed depending on the viscosity of the sample.

[0097] Place the rotor vertically into the center of the sample and bring the liquid level to the rotation level mark.

[0098] The sample and the rotor in the test container are thermostatted to 25 ± 0.5 °C and the sample temperature is kept uniform.

[0099] Start rotating, and wait until the pointer of the instrument is stable before reading the value and converting.

[0100] 2.1 Viscosity test;

[0101] The fermented facial mask liquids of Examples 1-5 and Comparative Examples 1-5 were subjected to viscosity tests;

[0102] The above viscosity is shown in Table 2:

[0103] Table 2 Viscosity test results Unit: mPa·s

[0104] Viscosity Example 1 500 Example 2 1536 Example 3 347 Example 4 757 Example 5 290 Comparative Example 1 567 Comparative Example 2 142 Comparative Example 3 1439 Comparative Example 4 2951 Comparative Example 5 843

[0105] When 1 kg of the fermented facial mask liquid with a viscosity of 500 mPa·s in Example 1 is diluted to a viscosity of 400 mPa·s, 33 g of water needs to be added, and when it is diluted to a viscosity of 250 mPa·s, 130 g of water needs to be added.

[0106] It can be seen from Examples 1-5 that the viscosity of the product obtained by fermentation using the method of the present invention is controllable; it can be seen from Comparative Example 1 that the viscosity can also be controlled by using only wheat germ, and it can be seen from Comparative Example 2 that the viscosity is low when only a small amount of rice is used, and it can be seen from Comparative Examples 3-5 that the viscosity is poorly controllable when fermented with brewer's yeast, especially in Comparative Examples 3 and 4, which have too high viscosities and require the addition of a large amount of water, but excessive dilution can easily lead to a low level of active ingredients, thereby reducing the effectiveness of the mask.

[0107] Therefore, for the present invention, the higher the viscosity, the better, nor the lower the viscosity, the better. It is more important to control the viscosity within an appropriate range.

[0108] 3. Elastase inhibition test

[0109] Control and test sample treatment:

[0110] Sample: the fermented facial mask liquid of Examples 1 to 5 and Comparative Examples 1 to 5.

[0111] Positive control (EGCG): 0.1% epigallocatechin gallate (EGCG, 98%) aqueous solution.

[0112] Negative control: pure water.

[0113] Test operation steps

[0114] Set up sample group, sample background group, solvent group and solvent background group. Each group needs to set up 3 parallels. Add different reagent solutions into the 96-well plate, shake gently, incubate at 25℃ for 15 minutes, place it in the microplate reader, and measure the absorbance at 410nm.

[0115] Calculation formula

[0116]

[0117] Where: A—is the absorbance of the reaction solution without sample;

[0118] B—is the absorbance of the reaction solution without sample and enzyme;

[0119] C—is the absorbance of the reaction solution containing sample and enzyme;

[0120] D—is the absorbance of the reaction solution containing sample and without enzyme.

[0121] 4. Hyaluronidase inhibition rate test

[0122] Control and test sample treatment

[0123] Sample: the fermented facial mask liquid of Examples 1 to 5 and Comparative Examples 1 to 5.

[0124] Positive control: 3% dipotassium glycyrrhizinate aqueous solution (dipotassium glycyrrhizinate, purity ≥98%).

[0125] Negative control: pure water.

[0126] Test operation steps

[0127] Set up a sample group, a sample background group, a solvent group and a solvent background group. Each group needs to have 3 parallels. Add different reagent solutions to the four groups respectively, shake well, and leave at room temperature for 30 minutes for color development. Use a UV spectrophotometer to measure the absorbance value at a wavelength of 528nm.

[0128] Calculation formula

[0129]

[0130] Where: A—is the absorbance of the reaction solution without sample;

[0131] B—is the absorbance of the reaction solution without sample and enzyme;

[0132] C—is the absorbance of the reaction solution containing sample and enzyme;

[0133] D—is the absorbance of the reaction solution containing sample and without enzyme.

[0134] 5. DPPH free radical scavenging rate test

[0135] Control and test sample treatment:

[0136] Sample: the fermented facial mask liquid of Examples 1 to 5 and Comparative Examples 1 to 5.

[0137] Positive control: 0.1% vitamin E (purity ≥ 95%) in ethanol (95%) solution.

[0138] Negative control: pure water.

[0139] Test operation steps

[0140] Set up sample tubes, sample background tubes, DPPH tubes, and solvent background tubes. Set up 3 parallel tubes for each group. Add different reagent solutions to the four groups, shake gently, and let stand at room temperature for 5 minutes. Transfer the reaction solutions of each group into a 1 cm cuvette and measure the absorbance at 517 nm.

[0141] Calculation formula

[0142]

[0143] Where:

[0144] T—is the absorbance value of the sample tube, that is, the absorbance value of the solution after the sample reacts with DPPH;

[0145] T0—is the background absorbance of the sample;

[0146] C—is the absorbance value of the DPPH tube three times in parallel, that is, the absorbance value of the DPPH solution when no sample is added;

[0147] C0—is the background absorbance of the solution.

[0148] Test Results

[0149] The test results are shown in Table 3;

[0150] Table 3 Test results of elastase inhibition rate, hyaluronidase inhibition rate and DPPH free radical scavenging rate

[0151] project Elastase inhibition rate% Hyaluronidase inhibition rate% DPPH free radical scavenging rate% Example 1 42.353±2.038 54.079±2.281 37.219±2.390 Example 2 40.824±2.252 51.216±1.870 36.589±3.254 Example 3 28.141±0.564 25.641±0.836 23.624±0.825 Example 4 31.011±0.359 29.119±3.252 26.119±1.611 Example 5 40.526±1.211 40.918±1.064 32.424±1.148 Comparative Example 1 39.608±0.414 41.147±3.121 31.868±2.663 Comparative Example 2 35.009±1.570 36.852±1.714 29.108±4.141 Comparative Example 3 24.822±3.411 25.378±4.186 24.605±2.161 Comparative Example 4 32.335±2.074 28.097±6.035 26.780±0.415 Comparative Example 5 13.240±1.161 15.539±0.882 18.712±3.302 Negative control -5.018±1.329 -2.167±2.5374 -1.751±0.082 Positive Control 86.428±2.524 65.31±0.8629 88.305±1.487

[0152] Result analysis:

[0153] 1. It can be seen from the test results of elastase inhibition rate that the products of Examples 1-5 all have a certain elastase inhibition rate; the test results of Comparative Examples 1 and 2 show that when wheat germ and rice are kept to a certain amount, they all have a certain elastase inhibition rate effect and have a better skin firming improvement effect; it can be seen from Comparative Examples 3-5 that the expected effect cannot be achieved after changing the strain; this conclusion is consistent with the conclusion of the applicant's prior application CN117530911A on a snow lotus fermentation product and its preparation method, application and cosmetics; the possible reason is that the strain has unique functionality in the efficacy of elastase.

[0154] 2. It can be seen from the test results of hyaluronidase inhibition rate that the products of Examples 1-5 all have a certain hyaluronidase inhibition rate; from the comparison of Example 1, Comparative Example 1 and Comparative Example 2, it can be seen that the comprehensive use of wheat germ and rice composition fermentation can effectively improve the soothing effect, indicating that in terms of hyaluronidase inhibition rate, the factors of wheat germ and rice raw materials play a more important role. Of course, it can be seen from Comparative Examples 3-5 that after using other strains, the hyaluronidase inhibition rate will also be significantly reduced, indicating that the role of strains is equally important.

[0155] 3. From the perspective of DPPH free radical scavenging rate, its results are similar to those of hyaluronidase inhibition rate.

[0156] Based on the above analysis, the use of Qibaizuan yogurt fermentation bacteria 10-type bifidobacterium combined with wheat germ and rice can have comprehensive advantages in elastase inhibition rate, hyaluronidase inhibition rate and DPPH free radical scavenging rate.

[0157] It will be apparent to those skilled in the art that the invention is not limited to the details of the exemplary embodiments described above and that the invention can be implemented in other specific forms without departing from the spirit or essential features of the invention. Therefore, the embodiments should be considered exemplary and non-limiting in all respects, and the scope of the invention is defined by the appended claims rather than the foregoing description, and it is intended that all variations falling within the meaning and scope of the equivalent elements of the claims be included in the invention. Any reference numeral in a claim should not be considered as limiting the claim to which it relates.

Claims

1. A method for preparing a fermented facial mask liquid, characterized in that: The method is specifically as follows: preparing a fermentation culture broth, and adjusting the pH of the fermentation culture broth to 7.0; Each kg of fermentation culture medium contains 20g of glucose, 10g of Tremella extract, 5g of yeast extract, 5g of wheat germ, and 10g of rice; After preparation, the fermentation culture solution is sterilized; After the fermentation culture liquid is cooled, the composite fermentation bacteria is added to the sterilized fermentation culture liquid at a ratio of 0.5 g of composite fermentation bacteria per 1 kg of fermentation culture liquid; the constant temperature of the fermentation tank is set to 37±1°C, the dissolved oxygen content is set to 30%, the stirring speed is linked to the dissolved oxygen content, the speed is automatically compensated and adjusted, and the fermentation culture time is 48 hours; After the fermentation culture is completed, the culture solution is sterilized; after the sterilization, the fermentation culture solution is processed using a centrifuge, and the culture supernatant after centrifugation is collected; The culture supernatant is clarified to prepare a fermented facial mask liquid; The fermentation bacteria is a 10-bacteria type bifidobacterium probiotic powder household lactic acid bacteria fermentation agent provided by Angel Yeast Co., Ltd. The viscosity of the fermented facial mask liquid is equal to 500 mPa·s without dilution.

2. A method for preparing a fermented facial mask liquid, characterized in that: The method is specifically as follows: preparing a fermentation culture broth, and adjusting the pH of the fermentation culture broth to 7.0; Each kg of fermentation culture solution contains 10g of glucose, 20g of Tremella extract, 1g of yeast extract, 2g of wheat germ, 30g of rice, and 0.3g of composite fermentation bacteria; After preparation, the fermentation culture solution is sterilized; After the fermentation culture liquid is cooled, the composite fermentation bacteria is added to the sterilized fermentation culture liquid at a ratio of 0.5 g of composite fermentation bacteria per 1 kg of fermentation culture liquid; the constant temperature of the fermentation tank is set to 37±1°C, the dissolved oxygen content is set to 30%, the stirring speed is linked to the dissolved oxygen content, the speed is automatically compensated and adjusted, and the fermentation culture time is 48 hours; After the fermentation culture is completed, the culture solution is sterilized; after the sterilization, the fermentation culture solution is processed using a centrifuge, and the culture supernatant after centrifugation is collected; The culture supernatant is clarified to prepare a fermented facial mask liquid; The fermentation bacteria is a 10-bacteria type bifidobacterium probiotic powder household lactic acid bacteria fermentation agent provided by Angel Yeast Co., Ltd. The viscosity of the fermented facial mask liquid is equal to 290 mPa·s without dilution.

3. A fermented facial mask liquid, characterized in that: The method is prepared according to claim 1 or 2.

4. The purpose of preparing a facial mask using the fermented facial mask liquid as claimed in claim 3.

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