A method for promoting tea tree growth and / or improving tea tree quality

Abstract

This invention belongs to the field of agricultural production technology, specifically relating to a method for promoting tea tree growth and / or improving tea tree quality. This invention supplements the tea trees with different light qualities from before bud break to the tea harvesting period, maintaining a daily light exposure time of 14–16 hours and a light intensity ≥200 μmol·m⁻². ‑2 ·s ‑1 This invention promotes the growth of tea trees; red light primarily promotes the growth of tea trees and the germination of tea buds, artificially extending the harvesting period of albino tea trees; while blue light primarily improves the quality of tea leaves, increasing the content of amino acids, mainly theanine, and enhancing the freshness of the tea. At the same time, the technical solution of this invention finds a balance between promoting the growth of tea trees and improving the quality of tea trees, thus providing a scientific basis for improving the cultivation environment of albino tea trees.

Description

Technical Field

[0001] This invention belongs to the field of agricultural production technology, and specifically relates to a method for promoting tea tree growth and / or improving tea tree quality. Background Technology

[0002] 'Baiye No. 1' is an albino tea tree variety, originating from Huzhou, Zhejiang, China. Baiye No. 1 is characterized by its delicately white, milky-white early spring buds, a mellow, sweet, and refreshing tea liquor, and its diuretic, blood pressure-lowering, and lipid-lowering effects, making it a favorite among consumers.

[0003] However, the harvesting period for albino tea trees is only about 10 days before Qingming Festival. Their short albino period is unfavorable for the harvesting and processing of white tea, thus limiting yield. Although existing technologies include research on using LED light sources to regulate plant growth performance, improper use of light sources can alter the photosynthesis and chlorophyll synthesis of tea trees, potentially leading to changes in leaf color, reduced growth, and ultimately hindering the improvement of albino tea quality. Summary of the Invention

[0004] The purpose of this invention is to provide a method for promoting the growth of tea trees and / or improving the quality of tea trees. By properly irradiating the trees with sunlight, it is possible not only to promote the early budding of albino tea trees and extend the harvesting period of albino tea trees, but also to improve the quality of white tea.

[0005] This invention provides a method for promoting tea tree growth and / or improving tea tree quality, comprising: from before tea tree budding to the tea tree harvesting period, under a light intensity ≥200 μmol·m -2 ·s -1 Tea trees are cultivated in a suitable growing environment; the tea trees are in the pre-budding to budding stage, and the light intensity in the growing environment is <200 μmol·m. -2 ·s -1 Supplement with light source #1; from the time the tea trees sprout until harvest, the light intensity in the growing environment should be <200 μmol·m⁻². -2 ·s -1 At the same time, a second light source is added; both the first and second light sources include red light, blue light, and white light; the light quality ratio of red light, blue light, and white light in the first light source is 7-8:1-2:1-2; the light quality ratio of red light, blue light, and white light in the second light source is 1-2:7-8:1-2; the photoperiod during cultivation is 14-16 hours / day.

[0006] Preferably, promoting tea tree growth includes: promoting earlier budding of tea trees and / or extending the tea tree harvesting period; improving tea tree quality includes increasing the amino acid content of tea leaves.

[0007] Preferably, the light exposure period during cultivation includes 6:00 to 22:00.

[0008] Preferably, the time before germination includes at least 20 to 30 days before germination.

[0009] Preferably, the wavelength of the blue light is 455–460 nm; the wavelength of the red light is 660–750 nm; and the wavelength of the white light is 400–700 nm.

[0010] Preferably, the tea tree harvesting period includes the tea tree with one bud and one leaf or the tea tree with one bud and two leaves.

[0011] Preferably, light source 1 or light source 2 is an LED light source.

[0012] Preferably, the tea tree includes albino tea trees.

[0013] Preferably, the albino tea tree includes 'Baiye No. 1'.

[0014] Beneficial Effects: This invention provides a method for promoting tea tree growth. This method involves maintaining a daily light exposure of 14–16 hours and a light intensity ≥200 μmol·m² from before tea bud break until the tea harvest period. -2 ·s -1 This enhances the photosynthetic performance of tea trees, which is beneficial for promoting their growth; when the light intensity in the planting environment is <200 μmol·m -2 ·s -1 At the same time, different light sources of different qualities are supplemented for different growth stages of tea trees. Red light is mainly used to promote the growth of tea trees and the germination of tea buds, thus artificially extending the harvesting period of albino tea trees. Blue light is mainly used to improve the quality of tea leaves, increase the content of amino acids, mainly theanine, and enhance the freshness of the tea leaves. At the same time, this invention finds a balance between promoting the growth of tea trees and improving the quality of tea trees. It improves the photosynthetic efficiency of tea trees while improving the quality of tea trees. In particular, it does not cause the tea leaves of albino tea trees to turn green, providing a scientific basis for improving the cultivation environment of albino tea trees. Attached Figure Description

[0015] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the accompanying drawings used in the embodiments will be briefly described below.

[0016] Figure 1 The changes in agronomic traits of tea trees in Example 1 and Comparative Example 1;

[0017] Figure 2 The sensory evaluation results in Example 1 and Comparative Example 1 demonstrate that supplemental lighting technology enhances the flavor of tea.

[0018] Figure 3 The theanine content in Example 1 and Comparative Example 1;

[0019] Figure 4 The content of different amino acids in Example 1 and Comparative Example 1;

[0020] Figure 5 This is a schematic diagram of the manually set supplementary system in Example 2;

[0021] Figure 6 The theanine content in tea leaves in Example 2. Detailed Implementation

[0022] This invention provides a method for promoting tea tree growth and / or improving tea tree quality, comprising: from before tea tree budding to the tea tree harvesting period, under a light intensity ≥200 μmol·m -2 ·s -1 Tea trees are cultivated in a suitable growing environment; the tea trees are in the pre-budding to budding stage, and the light intensity in the growing environment is <200 μmol·m. -2 ·s -1 Supplement with light source #1; from the time the tea trees sprout until harvest, the light intensity in the growing environment should be <200 μmol·m⁻². -2 ·s -1 At the same time, a second light source is added; both the first and second light sources include red light, blue light, and white light; the light quality ratio of red light, blue light, and white light in the first light source is 7-8:1-2:1-2; the light quality ratio of red light, blue light, and white light in the second light source is 1-2:7-8:1-2; the photoperiod during cultivation is 14-16 hours / day.

[0023] The present invention preferably obtains tea trees to be cultivated. In the present invention, the tea trees preferably include albino tea trees; the variety of the albino tea trees is preferably 'Baiye No. 1'; there are no special requirements for the source or age of the tea trees.

[0024] After obtaining the tea trees to be cultivated, from before the tea trees sprout until the tea trees sprout, place the tea trees in a light intensity ≥200 μmol·m -2 ·s -1 The tea plants are cultivated in a suitable growing environment to obtain sprouted tea trees. This invention is preferably carried out under a light intensity of 200 μmol·m⁻². -2 ·s -1 ~1300 μmol·m -2 ·s -1 Cultivated in a suitable growing environment, and further optimized to 200 μmol·m -2 ·s -1 ~600 μmol·m -2 ·s -1 More preferably 200 μmol·m -2 ·s -1 ~300 μmol·m-2 ·s -1 The optimal value is 300 μmol·m -2 ·s -1 The tea tree budding time is preferably 20-30 days before budding; there are no special requirements for the criteria for determining tea tree budding, and standards well known in the art can be used.

[0025] When the cultivation is carried out before the tea tree buds sprout and during the sprouting period, the preferred cultivation conditions include photoperiod, photoperiod duration, temperature of the planting environment, and humidity of the planting environment; the photoperiod is 14-16 h / d, preferably 15-16 h / d, and more preferably 16 h / d; the photoperiod duration is preferably 6:00-22:00, that is, the tea tree preferably receives light intensity ≥200 μmol·m² from 6:00 to 22:00 every day. -2 ·s -1 The plants are cultivated in a suitable growing environment; the light exposure period is preferably from 6:00 to 20:00, that is, the light exposure time preferably begins at 6:00 every day. During this stage, the temperature of the growing environment is preferably 4 to 25°C, more preferably 10 to 22°C, and even more preferably 10 to 20°C; the humidity of the growing environment is preferably 50% to 95%, more preferably 60% to 90%, and even more preferably 70% to 85%.

[0026] During the period from before the tea tree buds sprout to when the light intensity in the planting environment is <200 μmol·m -2 ·s -1 In this invention, light source No. 1 is used for supplemental lighting. In this invention, light source No. 1 includes red light, blue light, and white light; the light quality ratio of red light, blue light, and white light in light source No. 1 is 7-8:1-2:1-2, more preferably 8:1:1; the wavelength of blue light in light source No. 1 is preferably 455-460nm, the wavelength of red light is preferably 660-750nm, and the wavelength of white light is preferably 400-700nm. The light source in this invention is preferably a light source generated by LED lamps, i.e., red light is generated by LED red lamps, blue light by LED blue lamps, and white light by LED white lamps; when using LED lamps, there are no special requirements for the arrangement of the LED red lamps, LED blue lamps, and LED white lamps, as long as the light quality in the light source can be evenly distributed; there are no special requirements for the source of the LED lamps, the distance between the LED lamps and the tea trees, or the installation method of the LED lamps, as long as the light intensity of the planting environment is ≥200μmol·m². -2 ·s -1Therefore, supplementing with light source No. 1 during this stage is beneficial to the growth of tea buds. In a specific embodiment of the present invention, the LED light was purchased from Wuhan Ye Dongli Biotechnology Co., Ltd.; because in this specific embodiment of the present invention, the ambient light intensity is between 0 and 1200 μmol·m⁻² during the period from 6:00 to 22:00 every day. -2 ·s -1 Within the range, therefore when the ambient light intensity is <200 μmol·m -2 ·s -1 Supplemental lighting is required at times; when supplemental lighting is required, the intensity of the No. 1 light source is 200–400 μmol·m⁻². -2 ·s -1 More preferably, it is 250–350 μmol·m -2 ·s -1 More preferably 300 μmol·m -2 ·s -1 This ensures that the light intensity of the planting environment is ≥200 μmol·m⁻². -2 ·s -1 Requirements.

[0027] After the tea trees have sprouted, the present invention places the tea trees under a light intensity of ≥200 μmol·m² from the time of sprouting until the tea harvesting period. -2 ·s -1 The plants are cultivated in a suitable growing environment. This invention is preferably carried out under a light intensity of 200 μmol·m⁻². -2 ·s -1 ~1300 μmol·m -2 ·s -1 Cultivated in a suitable growing environment, and further optimized to 200 μmol·m -2 ·s -1 ~600 μmol·m -2 ·s -1 More preferably 200 μmol·m -2 ·s -1 ~300 μmol·m -2 ·s -1 The optimal value is 300 μmol·m -2 ·s - The preferred tea tree harvesting period includes the tea tree with one bud and one leaf or one bud and two leaves. There are no special requirements for the criteria for determining the germination period and harvesting period of the tea tree in this invention; any criteria well known in the art can be used.

[0028] From the time the tea trees sprout until the tea trees are harvested, when the light intensity in the planting environment is <200 μmol·m -2 ·s -1In this invention, light source No. 2 is used for supplemental lighting. In this invention, light source No. 2 includes red light, blue light, and white light; the light quality ratio of red light, blue light, and white light in light source No. 2 is 1-2:7-8:1-2, more preferably 1:8:1; the wavelengths, sources, and installation methods of the blue light, red light, and white light in light source No. 2 are the same as those of light source No. 1, and will not be repeated here; simultaneously, the cultivation conditions at this stage are the same as those for the cultivation from before tea bud break to the tea tree sprouting period, and the cultivation conditions from before tea bud break to the tea tree sprouting period have been described in detail above, and will not be repeated here. Supplementing with light source No. 2 after tea tree sprouting to the tea tree harvesting period is beneficial to increasing the content of amino acids, mainly theanine, in the buds and leaves. In the specific embodiment of this invention, the light intensity in the environment is 0-1200 μmol·m² during the period from 6:00 to 22:00 every day. -2 ·s -1 Within the range, therefore when the ambient light intensity is <200 μmol·m -2 ·s -1 Supplemental lighting is required at times; when supplemental lighting is required, the intensity of the second light source is 200–400 μmol·m⁻². -2 ·s -1 More preferably, it is 250–350 μmol·m -2 ·s -1 More preferably 300 μmol·m -2 ·s -1 This ensures that the light intensity of the planting environment is ≥200 μmol·m⁻². -2 ·s -1 Requirements.

[0029] The method of promoting tea tree growth according to the present invention preferably includes: promoting early budding of tea trees and / or extending the harvesting period of tea trees, more preferably including promoting early budding of tea trees and extending the harvesting period of tea trees; the method of improving tea quality includes increasing the amino acid content of tea leaves; the types of amino acids preferably include one or more of theanine, serine, leucine, tyrosine, phenylalanine, histidine and arginine.

[0030] In summary, the technical solution provided by this invention promotes the growth of tea trees and improves the quality of tea by supplementing different light qualities at different growth stages of tea trees. Specifically, the first stage mainly uses red light to promote the growth of tea trees and the sprouting of tea buds, which is conducive to harvesting tea trees 3-5 days earlier, artificially extending the harvesting period of Anji white tea, while increasing the average yield and economic benefits. The second stage mainly uses blue light to improve the quality of tea leaves, increase the content of amino acids, mainly theanine, and improve the freshness of tea leaves, without significantly affecting the whitening degree of leaves.

[0031] To further illustrate the present invention, the following detailed description, in conjunction with the accompanying drawings and embodiments, describes a method for promoting tea tree growth and / or improving tea tree quality provided by the present invention, but these descriptions should not be construed as limiting the scope of protection of the present invention.

[0032] The standards referenced during the experiment are as follows:

[0033] Criteria for judging the development stage of tea buds: Refer to the "Quality Control Specifications for Tea Tree Germplasm Resource Data" to judge the growth and development stage of tea buds. That is, 100 overwintering buds are selected from each treatment group, tagged and tracked. The growth of overwintering buds is observed and recorded every 2 to 3 days. When more than 20 overwintering buds have developed to a certain growth and development stage, it is judged that the tea buds have developed to that stage.

[0034] Method for determining the length of one bud and three leaves: The length of one bud and three leaves was determined with reference to the "Quality Control Specification for Tea Germplasm Resource Data". That is, 30 one buds and three leaves were randomly picked from each treatment group. The picking standard was to pick from the fish leaf position of the new shoot, and measure from the base to the bud base (growing point) with a ruler. The data is expressed as the mean and standard deviation.

[0035] 100-bud weight: The weight of 100 buds was determined according to the "Quality Control Specifications for Tea Germplasm Resource Data". This refers to the picking standard based on the measurement of the length of three leaves from the same bud. 100 one-bud-three-leaf samples were picked from each treatment group and their weight was calculated. Rain-soaked leaves and dew-soaked leaves cannot be used as samples, and they must be weighed within one hour after sampling.

[0036] Theanine detection method: The sample was thoroughly ground into powder in a mortar and freeze-dried for at least 48 hours using a vacuum freeze dryer. 0.05 g of the sample powder was placed in a 1.5 mL centrifuge tube and dissolved in 1 mL of Watson's purified water. The mixture was extracted in a 100℃ water bath for 30 min, with venting and vortexing every 10 min. At least three technical replicates were performed for each sample. After the water bath, the sample was centrifuged at 12000 rpm for 15 min. The supernatant was collected and filtered through a 0.22 μm aqueous phase filter into a vial for HPLC analysis. Analytical conditions: Waters e2695, Symmetry C18 reversed-phase column. Mobile phase A: Watson's water; Mobile phase B: pure acetonitrile. From 0 to 7 min, phase A was 0-100%, and phase B was 0%; from 7 to 9 min, phase A decreased from 100% to 40%, and phase B increased from 0% to 60%; from 9 to 15 min, phase A increased from 40% to 100%, and phase B decreased from 60% to 0%; from 15 to 20 min, phase A was maintained at 100%, and phase B was 0%, with a flow rate of 1 mL / min and a column temperature of 28 °C. The excitation wavelength was 210 nm.

[0037] Method for detecting free amino acid content: Following the local Anji white tea picking standards and processing techniques, one bud and two leaves were picked from both the un-lit and lit-lit groups, and processed into Anji white tea using the same process. 100 mg of freeze-dried tea leaves were ground and ultrasonically extracted with 1 mL of 4% sulfosalicylic acid at 4℃ for 30 min. After centrifugation (13,000 rpm, 30 min), the supernatant was transferred to a new tube, and the precipitate was extracted again as described above. The supernatant was collected, measured to 2 mL, and then filtered through a 0.22 μm water membrane.

[0038] Free amino acids were detected using a high-speed amino acid analyzer (L-8900, Hitachi, Japan). The mobile phase consisted of lithium citrate, and UV-Vis was used for detection at 570 and 440 nm. The mobile phase flow rate was 0.35 mL / min. -1 The flow rate of the derivatization reagent was 0.3 mL / min. -1 Column temperature, post-column reaction unit temperature, and autosampler temperature were maintained at 38°C, 130°C, and 4°C, respectively. Injection volume was 20 μL. Theanine (Sigma Chemicals, St. Louis, Missouri, USA) and other amino acids (Waters, Milford, Massachusetts, USA) were used as standards for free amino acid calculations.

[0039] A supplemental lighting system is installed in the tea garden: The LED supplemental lighting system is preferably installed on a sunny slope (to facilitate photovoltaic power generation). Each supplemental lighting unit includes a light source bracket, photovoltaic panels, a photosensor, a timer, and two sets of LED light sources (two light sources #1 and two light sources #2). The light quality ratio of light source #1 is set as red (660–750 nm): blue (455–460 nm): white (400–700 nm) = 8:1:1, and the light quality ratio of light source #2 is set as red (660–750 nm): blue (455–460 nm): white (400–700 nm) = 1:8:1. Preliminary testing revealed that the light intensity in the planting environment ranges from 0 to 1200 μmol·m⁻². -2 ·s -1 Therefore, when the light intensity in the environment is <200 μmol·m -2 ·s -1 Supplemental lighting is required; the supplemental lighting intensities of light sources 1 and 2 are set to 200–300 μmol·m⁻², respectively. -2 ·s -1 This will meet the planting needs. Specifically, the distance between light source brackets 1 and 2 should be approximately 5-6 meters, the distance between the light sources and the tea tree leaves should be 2-3 meters, and the light coverage angle should be 120 degrees. This will ensure a light intensity of 200-300 μmol·m⁻² during the supplemental lighting process. -2 ·s -1 .

[0040] The aforementioned system can automatically supplement lighting by detecting the intensity of light sources in the environment. The supplementary lighting time is controlled by the light source detector. When the detected ambient natural light is <200 μmol·m -2 ·s -1 When the ambient natural light is ≥600 μmol·m, the supplemental lighting system will automatically turn on; -2 ·s -1 When the time is right, the supplemental lighting system will automatically turn off to ensure that the daily light exposure time is 14-16 hours.

[0041] The first stage involves supplemental lighting to promote tea tree growth and bud break: approximately 20 days before the bud break of the 'Baiye No. 1' tea trees (this may vary annually depending on local climate characteristics), light source No. 1 is turned on for supplemental lighting; the total supplemental lighting time is set at 16 hours / day (i.e., 6:00 AM to 10:00 PM daily), with the specific supplemental lighting time controlled by the light source detector. This is done when the ambient natural light is <200 μmol·m⁻². -2 ·s -1 When the ambient natural light is ≥600 μmol·m, the supplemental lighting system will automatically turn on; -2 ·s -1 When the time comes, the supplemental lighting system will automatically shut off; supplemental lighting will continue until the bud stage or the one bud and one leaf stage, with a cycle of approximately 20 days.

[0042] The second stage enhances tea flavor and quality through supplemental lighting: After tea buds sprout to the bud stage or the one-bud-one-leaf stage, turn off light source #1 and turn on light source #2 for supplemental lighting. Set the total supplemental lighting time to 16 hours / day (i.e., 6:00 AM to 10:00 PM daily). The specific supplemental lighting time is controlled by the light source detector. When the ambient natural light is <200 μmol·m⁻², supplemental lighting should be provided. -2 ·s -1 When the ambient natural light is ≥600 μmol·m, the supplemental lighting system will automatically turn on; -2 ·s -1 When the time comes, the supplemental lighting system will automatically turn off, and will continue to supplement the light until the tea tree grows to one bud and one leaf or one bud and two leaves.

[0043] Example 1 (referred to as supplementary lighting group)

[0044] A method for promoting tea tree growth and / or improving tea tree quality, comprising the following steps:

[0045] Experiment period: March 1-31, 2022

[0046] Location: Baiye No. 1 tea garden base of Anji Songming White Tea Co., Ltd., Anji County, Huzhou City, Zhejiang Province (the experimental site is 30.44°N, 119.38°E), the experimental plot area is 3m×20m;

[0047] From February 25th to 28th, supplemental lighting systems were installed in the tea gardens according to the aforementioned method.

[0048] Starting March 1st, only Light Source No. 1 will be supplemented as required, i.e., when the ambient natural light is <200 μmol·m⁻². -2 ·s -1 When the ambient natural light is ≥600 μmol·m, the supplemental lighting system automatically turns on light source #1; -2 ·s -1 At this time, the supplemental lighting system automatically shuts off light source #1 to meet the requirement of 16 hours of light per day, and the total daily light source concentration of light source #1 and natural light in the environment is between 200 and 1200 μmol·m⁻². -2 ·s -1 This meets the needs of planting.

[0049] On March 16, the tea buds reached the one bud and one leaf stage (judged according to the agronomic standards for tea bud development), so the No. 1 light source was stopped and the No. 2 light source was turned on.

[0050] On March 31, more than 20 tea buds had grown to the standard of one bud and three leaves. According to the agronomic standards for tea bud development, the tea buds in the supplemental lighting group had reached the one bud and three leaves stage. At this time, light source No. 2 was turned off, and the supplemental lighting ended.

[0051] Comparative Example 1 (referred to as the group without supplemental lighting)

[0052] The difference from Example 1 is that no supplemental lighting system was set up. Instead, a control group without sunlight was established within the tea garden, with an area of ​​3m × 20m. Observations on the tea trees began on March 1st.

[0053] On March 19, the tea buds reached the stage of one bud and one leaf (about 2 to 3 days later than in Example 1).

[0054] On March 31, the tea buds reached the stage of one bud and three leaves.

[0055] The growth of tea trees was recorded at different growth stages, and the results are shown in Table 1 and 2. Figure 1 The left image in the middle ( Figure 1 The left and middle figures show the growth of tea trees under different treatments; agronomic indicators such as the length of one bud and three leaves and the weight of 100 buds were measured on March 31, and the results are shown below. Figure 1 The right image in the middle ( Figure 1 The right-middle figure shows the physicochemical indicators of tea plants under different treatments, including the length of one bud and three leaves, the fresh weight of 100 buds, and the chlorophyll content.

[0056] Table 1. Growth of tea trees at different stages

[0057] deal with March 2 March 16 March 19 March 31 Supplemental lighting unit (Example 1) Unsprouted One bud and one leaf One bud and one leaf One bud with three leaves No supplemental lighting (Comparative Example 1) Unsprouted bud One bud and one leaf One bud with three leaves

[0058] From Table 1 and Figure 1It can be seen that the technical solution provided by the present invention can promote the growth of tea tree buds and increase the fresh weight of 100 buds and chlorophyll content; that is to say, the supplementary lighting technology provided by the present invention can increase the tea yield per unit area of ​​tea garden to a certain extent.

[0059] The supplementary lighting ended on March 31, 2022. On April 1, in accordance with the local Anji white tea picking standards and processing technology, one bud and two leaves of tea leaves from the non-supplementary lighting group (Comparative Example 1) and the supplementary lighting group (Example 1) were picked and processed into Anji white tea using the same process for subsequent sensory evaluation and internal substance detection.

[0060] Sensory evaluation: A total of 30 evaluators with tea sensory evaluation skills conducted the evaluation according to the "National Standard for Chinese Tea Evaluation Methods" (GB / T 23776-2018) for green tea. Each tea sample consisted of 3.0g of tea at a tea-to-water ratio (mass-volume ratio) of 1g:50mL. The tea was placed in an evaluation cup, filled with boiling water, covered, and steeped for 4 minutes. The tea liquor was then quickly filtered out, leaving the tea leaves in the cup. The evaluation proceeded in the order of liquor color, aroma, taste, and then the tea leaves. To ensure data reliability, the sensory evaluation experiment was independently repeated at least three times. The state of the tea leaves is shown in Table 2. Figure 2 ( Figure 2 The left image shows the dry tea leaves, the color of the tea liquor, and the appearance of the infused leaves; the right image shows the radar chart presentation of the sensory evaluation scores for the tea.

[0061] Table 2 Sensory evaluation results of different treatments

[0062] deal with soup color aroma taste Supplemental lighting unit (Example 1) tender green, bright Tender and long-lasting fragrance Pure and refreshing No supplemental lighting (Comparative Example 1) Green, relatively bright Shang Qingxiang The flavor is rather mild

[0063] From Table 2 and Figure 2 It can be seen that supplemental lighting can significantly enhance the fresh and crisp taste of tea, but has no significant effect on the color of the dry tea leaves, the color of the tea liquor, or the leaves after brewing.

[0064] On March 23, March 28, and March 31, samples of one bud and two leaves from the new shoots of tea trees in Example 1 (supplemented light group) and Comparative Example 1 (no supplemented light group) were collected, with each treatment performed in triplicate for the detection of theanine content. The results are shown in Table 3. Figure 3 and Figure 4 ; Figure 3 This indicates the theanine content at different times. Figure 4 This indicates the content of different amino acids at different times.

[0065] Table 3 Amino acid content in teas treated with different methods

[0066]

[0067]

[0068] Note: * indicates statistically significant differences in amino acids.

[0069] From Table 3, Figure 3 and Figure 4 It can be seen that the technical solution provided by the present invention can significantly increase the content of theanine, serine, leucine, tyrosine, phenylalanine, histidine and arginine in tea.

[0070] Example 2

[0071] To fully verify the effect of supplemental lighting, one-year-old tea tree cuttings (purchased from Niansheng Agricultural Seed Breeding Base in Chaohu City, Anhui Province) were illuminated in an artificial climate chamber (temperature 18–22℃, 6:00–18:00) using LED white lamps with a light intensity of 200–300 μmol·m⁻¹. -2 ·s -1 The LED white light will be turned off at 18:00. The illuminance after turning off the LED white light will be 200–300 μmol·m⁻². -2 ·s -1 A simulated tea garden supplemental lighting experiment was conducted, with the supplemental lighting system as follows: Figure 5 As shown ( Figure 5 The left image shows the white light supplementation scene and the control group; the right image shows the composite red light group and the composite blue light group supplementation scene. The steps are as follows:

[0072] 120 healthy, uniformly growing, one-year-old 'Baiye No. 1' cuttings were selected and divided into 4 treatment groups, with 30 cuttings in each group. The cuttings were then transplanted into plastic culture pots containing a seedling substrate made of peat moss, perlite, and vermiculite in a volume ratio of 2:1:1.

[0073] Control group: Illuminated with LED white lamps at an intensity of 200–300 μmol·m⁻¹ -2 ·s -1 The daily sunlight exposure is 12 hours, specifically from 6:00 to 18:00.

[0074] White light group: In addition to the control group, the supplemental white light was extended by 4 hours / day, that is, using an LED white light source with a wavelength of 400-700nm and a light source intensity of 200-300μmol·m. -2 ·s -1 Supplemental lighting for 4 hours daily from 18:00 to 22:00 is provided to ensure the tea trees receive 200–300 μmol·m⁻² of light daily. -2 ·s -1 Cultivate in the environment for 16 hours;

[0075] Composite Blue Light Group: In addition to the control group, the supplemental composite blue light was extended by 4 hours / day, i.e., the light quality ratio of LED red light: LED blue light: LED white light was 1:8:1, where the red light source wavelength was 660–750nm; the blue light source wavelength was 455–460nm; the white light source wavelength was 400–700nm; and the light intensity was 200–300 μmol·m⁻¹. -2 ·s -1 Supplemental lighting for 4 hours daily from 18:00 to 22:00 is provided to ensure the tea trees receive 200–300 μmol·m⁻² of light daily. -2 ·s -1 Cultivate in the environment for 16 hours;

[0076] Composite Red Light Group: In addition to the control group, the supplemental composite red light was extended by 4 hours / day, i.e., the light quality ratio of LED red light: LED blue light: LED white light was 8:1:1, with the red light source wavelength being 660–750 nm; the blue light source wavelength being 455–460 nm; and the white light source wavelength being 400–700 nm; the light intensity being 200–300 μmol·m⁻¹. -2 ·s -1 Supplemental lighting for 4 hours daily from 18:00 to 22:00 is provided to ensure the tea trees receive 200–300 μmol·m⁻² of light daily. -2 ·s -1 Cultivate in the environment for 16 hours.

[0077] Each group of tea seedlings was labeled on the surface of a plastic pot, and the four groups were separated by a black cloth to ensure that the tea seedlings were exposed to only one type of light for one month.

[0078] One month after the treatment, one bud and one leaf were collected from four groups of tea seedlings of the same size, freeze-dried, and their amino acid content was analyzed. The results are shown below. Figure 6 (exist Figure 6 In the diagram, C represents the control group; W represents the white light group; B represents the combined blue light group; and R represents the combined red light group; P < 0.05.

[0079] Depend on Figure 6 It can be seen that the theanine content of tea seedlings in the blue light supplementation group was significantly higher than that in other groups, verifying the effect of blue light supplementation in tea gardens on increasing the theanine content.

[0080] In summary, the technical solution provided by this invention promotes the growth of tea trees by supplementing them with different light qualities at different growth stages, which not only promotes the growth of tea trees but also improves the quality of tea.

[0081] Although the above embodiments have provided a detailed description of the present invention, they are only some embodiments of the present invention, and not all embodiments. People can obtain other embodiments based on these embodiments without creative effort, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A method for promoting tea tree growth and improving tea tree quality, characterized in that, include: Cultivate the tea tree in a planting environment with light intensity ≥200 μmol·m -2 ·s -1 from the budding stage to the picking stage of the tea tree; the time before budding is 20 days before budding; When the tea tree is in the pre-budding to budding stage, and the light intensity in the planting environment is <200 μmol·m -2 ·s -1 At that time, supplement with light source No. 1; From the time the tea trees sprout until harvest, the light intensity in the growing environment should be <200 μmol / m². -2 ·s -1 At that time, supplement with light source No. 2; Both light source No. 1 and light source No. 2 include red light, blue light and white light; The light quality ratio of red light, blue light and white light in the No. 1 light source is 8:1:1; The light quality ratio of red light, blue light and white light in the No. 2 light source is 1:8:1; The photoperiod during cultivation is 16 hours per day; the photoperiod during cultivation includes 6:00 to 22:

00. The promotion of tea tree growth includes: promoting early budding of tea trees and / or extending the tea tree harvesting period; The improvement of tea quality includes increasing the amino acid content of tea leaves, and the types of amino acids include one or more of theanine, serine, leucine, tyrosine, phenylalanine, histidine, and arginine. The first light source or the second light source is an LED light source; the supplementary light intensity of the first light source and the second light source is 200~300 μmol / m², respectively. -2 ·s -1 ; The tea tree harvesting period includes the tea tree with one bud and one leaf or the tea tree with one bud and two leaves; The tea trees include albino tea trees, and the albino tea trees include 'Baiye No. 1'.

2. The method according to claim 1, characterized in that, The wavelength of the blue light is 455~460nm; the wavelength of the red light is 660~750nm; and the wavelength of the white light is 400~700nm.

Images