A composition containing slow-release anti-dandruff and antipruritic effects, and its preparation method and application

Abstract

The present invention relates to a composition containing a slow-release anti-dandruff and antipruritic effect, and its preparation method and application. The present invention group is prepared by combining soybean extract, glabridin, maitake polysaccharide, grape seed extract, oat extract, pine pollen extract, and tea tree oil. Maitake polysaccharide has a promoting effect on the generation of hyaluronic acid, and the components such as phospholipids and lecithin in the soybean extract have a moisturizing effect. In the presence of pine pollen extract, the damaged barrier surface structure of the scalp can be repaired, the scalp barrier can be enhanced, and the stratum corneum barrier of the scalp and the hydration of the stratum corneum can be avoided from being destroyed, and symptoms such as scalp dandruff are further produced. In the present invention, ceramide is also used to coat the composition, sodium alginate-chitosan is used for coating, glutaraldehyde is modified on the surface of the microcapsule, and the aldehyde group of glutaraldehyde forms a Shiff base with the amino group of chitosan, which has a good antibacterial effect and can enhance the antibacterial effect of the composition.

Description

Technical Field

[0001] The invention belongs to the technical field of anti-dandruff and antipruritic, and specifically relates to a composition containing a packaged sustained-release anti-dandruff and antipruritic effect, and a preparation method and application thereof. Background Art

[0002] Dandruff is caused by the continuous peeling of the stratum corneum of the scalp, which is a result of metabolism. Under normal circumstances, the renewal cycle of the keratin cells of the scalp is 28 days. In one cycle, it starts to differentiate and grow from the bottom of the epidermis, then gradually moves to the surface of the scalp, and finally differentiates to form the stratum corneum. Under the action of related scalp metabolic enzymes, the stratum corneum cells will slowly fall off, forming tiny dandruff that is invisible to the naked eye. But when there is too much dandruff and the pores are blocked, it will easily cause hair weakness and bacteria to proliferate. The type of dandruff will also change from physiological dandruff to pathological. This type of dandruff is characterized by redness, swelling, heat and pain on the scalp, accompanied by symptoms such as itching and tightness.

[0003] Currently, the treatment methods for dandruff mainly focus on Malassezia, which is a fungus with lipase activity. When the scalp secretes too much sebum, the Malassezia on the scalp secretes lipase to break down the triglycerides and saturated fatty acids in the sebum into unsaturated fatty acids. Unsaturated fatty acids are pro-inflammatory and can penetrate into the stratum corneum of the scalp, not only causing itching of the scalp, but also allowing calcium ions to flow into the scalp cells, which will further lead to excessive keratinization of the scalp keratinocytes, thereby causing abnormal scalp keratinization and accelerating the production of dandruff.

[0004] However, there are many factors that affect dandruff, including scalp microecology, stratum corneum lipids, scalp barrier and other factors. It is not just the result of the action of Malassezia alone. On the other hand, after antifungal treatment, it relapses quickly, indicating that repeated use of anti-dandruff agents will produce drug resistance. Therefore, it is urgent to provide a long-lasting anti-dandruff and antipruritic product. Summary of the invention

[0005] The purpose of the present invention is to provide a composition containing a sustained-release anti-dandruff and antipruritic effect, which has a good effect in long-term anti-dandruff and antipruritic effect.

[0006] The second object of the present invention is to provide a method for preparing the above composition.

[0007] The third object of the present invention is to provide application of the above composition.

[0008] The purpose of the present invention can be achieved through the following technical solutions:

[0009] A composition with slow-release anti-dandruff and antipruritic effects, the raw materials include the following components in parts by weight:

[0010] 10-15 parts of soybean extract

[0011] Glabridin 5-10 parts

[0012] 15-20 parts of Grifola frondosa polysaccharide

[0013] Grape seed extract 10-15 parts

[0014] 5-10 parts oat extract

[0015] 5-10 parts of pine pollen extract

[0016] 2-5 parts of tea tree oil.

[0017] As a preferred embodiment of the present invention, the grape seed extract is obtained by extraction with 50 wt % ethanol aqueous solution at 20-25°C.

[0018] As a preferred embodiment of the present invention, the oat extract is obtained by extraction with 80 wt % ethanol aqueous solution at 68-72°C.

[0019] As a preferred embodiment of the present invention, the pine pollen extract is prepared by water extraction and alcohol precipitation, wherein the solid-liquid ratio during the water extraction process is 1:25-30 and the temperature is 48-50°C; and 3-5 times of 75wt% ethanol aqueous solution is used during the alcohol precipitation process.

[0020] The preparation method of the above composition comprises the following operations:

[0021] Preparing prefabricated microspheres: mixing the raw material of the composition with ceramide to obtain a mixture, stirring the mixture, ultrasonicating the mixture, and then centrifuging to obtain prefabricated microspheres;

[0022] Preparation of the composition: The prefabricated microspheres are sequentially mixed with sodium dodecyl sulfate, sodium alginate, chitosan and glutaraldehyde, and allowed to stand to obtain the composition.

[0023] As a preferred embodiment of the present invention, in the preparation of prefabricated microspheres, the mass ratio of the raw material to ceramide is 1:1 to 1.2.

[0024] As a preferred embodiment of the present invention, in preparing the composition, the sodium dodecyl sulfate solution, sodium alginate solution, chitosan solution and glutaraldehyde solution are added at a mass concentration of 5.0%, 3.0%, 3.0% and 2.0% respectively.

[0025] As a preferred embodiment of the present invention, the dosage ratio of the prefabricated microspheres to the sodium dodecyl sulfate solution, the sodium alginate solution, the chitosan solution and the glutaraldehyde solution is 2kg: 0.8-1mL: 1.8-2.2mL: 3.8-4.2mL: 1.8-2.2mL.

[0026] The composition is used as an active ingredient in anti-dandruff and antipruritic products.

[0027] Beneficial effects of the present invention:

[0028] 1. The composition of the present invention is prepared by combining soybean extract, glycyrrhizin, maitake mushroom polysaccharide, grape seed extract, oat extract, pine pollen extract and tea tree oil. Among them, tea tree oil has a broad-spectrum antibacterial effect and can achieve microecological regulation through antibacterial effect; soybean extract is rich in active ingredients such as isoflavones, has antioxidant and anti-inflammatory effects, and helps to relieve itching caused by scalp inflammation; the phenolic alkali in oat extract has anti-inflammatory, antipruritic and soothing effects, can effectively relieve scalp irritation and have an antipruritic effect; glycyrrhizin, maitake mushroom polysaccharide and grape seed extract work together to help scalp anti-inflammatory, help relieve scalp inflammation and the itching caused by it, improve the scalp environment, and reduce dandruff regeneration. In addition, the grifola frondosa polysaccharide promotes the production of hyaluronic acid, and the phospholipids, lecithin and other components in the soybean extract have a moisturizing effect. In the presence of pine pollen extract, it can significantly promote the proliferation and division of skin stem cells and cell migration, repair the damaged barrier surface structure of the scalp, strengthen the scalp barrier, and avoid the stratum corneum barrier and hydration of the stratum corneum from being damaged, further causing symptoms such as scalp dandruff. In the present invention, ceramide is also used to coat the composition, and ceramide further protects the scalp barrier.

[0029] 2. In the present invention, the composition is coated with sodium alginate-chitosan. There are active groups such as hydroxyl and amino groups at the ends of sodium alginate and chitosan molecules, which form hydrogen bonds with the components of the composition. The amino groups on the chitosan molecular chain and the carboxyl groups on the sodium alginate molecular chain are electrostatically attracted to each other, and the surfaces of the ceramide composition microspheres are coated in the form of a double electric layer. Glutaraldehyde is used to modify the surface of the microcapsules. The aldehyde groups of glutaraldehyde and the amino groups of chitosan form a Shiff base, which has a good antibacterial effect and can enhance the antibacterial effect of the composition. A cross-linked layer is formed on the surface of the chitosan and composition microspheres, and then a polyelectrolyte membrane is formed, thereby controlling the release behavior of the microcapsules. The prepared microcapsule composition has a good sustained-release effect. DETAILED DESCRIPTION

[0030] The present invention is further described in detail below in conjunction with specific implementation methods.

[0031] The following soybean extracts were purchased from Shouyang Haoyuan Biotechnology Co., Ltd., batch number: HY20211220; glabridin was purchased from Nanjing Puyi Biotechnology Co., Ltd., batch number: PY0233; tea tree oil was purchased from Guangdong Fuyang Biotechnology Co., Ltd., batch number: 20190916; Grifola frondosa polysaccharide was purchased from Shanghai Xige Biotechnology Co., Ltd., item number: XGR8785; porous starch was purchased from Liaoning Lida Biotechnology Co., Ltd., and the following will not be repeated.

[0032] Example 1

[0033] A composition with slow-release anti-dandruff and antipruritic effects is prepared by the following specific steps:

[0034] Preparation of grape seed extract: crush the grape seeds through an 80-mesh sieve, weigh 50 kg and dissolve them in a 50wt% ethanol aqueous solution, let it stand at 20°C for 2 hours, then transfer it to a centrifuge and centrifuge it at 1200r / min for 15 minutes. After the end, take the supernatant. Wash the residue after centrifugation once with a small amount of 50wt% ethanol aqueous solution, mix the washing liquid and the supernatant, heat to remove the ethanol, and obtain a water extract. Extract the water extract with petroleum ether, and after extraction, put the water layer solution into a heated water bath for concentration, and then dry it in an oven at 70°C to obtain the grape seed extract, which is refrigerated at 0-4°C for use.

[0035] Preparation of oat extract: grind oats through a 100-mesh sieve, weigh 30 kg and dissolve in 80 wt% ethanol aqueous solution, stir and extract at 68°C for 2 hours, then transfer to a centrifuge and centrifuge at 4000 r / min for 15 minutes, and take the supernatant after the end. Wash the residue after centrifugation once with a small amount of 80 wt% ethanol aqueous solution, mix the washing liquid and the supernatant, filter under negative pressure, and then concentrate by rotary evaporation at 58°C to constant weight to obtain the oat extract, which is refrigerated at 0-4°C for later use.

[0036] Preparation of pine pollen extract: 30 kg of pine pollen is weighed through a 200-mesh sieve, and pine pollen and water are added at a solid-liquid ratio of 1:25, and ultrasonic reflux extraction is performed at 48°C, 40W, and 40KHz for 2 hours, and the residue after ultrasonic reflux is subjected to secondary extraction, and the filtrates obtained twice are collected; the filtrate is concentrated under reduced pressure at 58°C to an extract, and a 75wt% ethanol aqueous solution with a mass of 3 times that of the concentrated solution is added, stirred for 10 minutes, and allowed to stand for alcohol precipitation for 12 hours, and the alcohol precipitation liquid is filtered, and the obtained filtrate is concentrated under reduced pressure at 60°C to an extract; and a 95wt% ethanol aqueous solution with a mass of 3 times that of the concentrated solution is added, stirred for 10 minutes, and allowed to stand for alcohol precipitation for 12 hours, and the alcohol precipitation liquid is filtered, and the obtained filter residue is sprayed with anhydrous ethanol, and then freeze-dried and ground into powder to obtain the pine pollen extract.

[0037] 1 kg of soybean extract, 0.5 kg of glabridin, 1.5 kg of maitake polysaccharide, 1 kg of grape seed extract, 0.5 kg of oat extract, 0.5 kg of pine pollen extract, and 0.2 kg of tea tree oil were weighed respectively, mixed, and then mixed with ceramide at a mass ratio of 1:1 to obtain a mixture, the mixture was stirred at high speed for 30 min at high temperature with a magnetic stirrer, then ultrasonicated for 30 min, and then centrifuged at 10000 r / min for 30 min, and the precipitate was washed 3 times with anhydrous ethanol to obtain prefabricated microspheres;

[0038] Add 0.8 L of 5.0% sodium dodecyl sulfate solution to 2 kg of prefabricated microspheres, stir in a constant temperature water bath at 30°C until uniform, continue to add 1.8 L of 3.0% sodium alginate solution, and stir at high speed; then add 3.8 L of 3.0% chitosan solution, continue stirring for 30 minutes to prepare a microcapsule solution, take 1.8 L of 2.0% glutaraldehyde solution and slowly add it dropwise to the microcapsule solution, let it stand for 24 hours, filter and dry, and thus prepare a composition with encapsulated sustained-release anti-dandruff and antipruritic effects.

[0039] Example 2

[0040] A composition with slow-release anti-dandruff and antipruritic effects is prepared by the following specific steps:

[0041] Preparation of grape seed extract: crush the grape seeds through an 80-mesh sieve, weigh 50 kg and dissolve them in a 50wt% ethanol aqueous solution, extract at 22°C for 2 hours, then transfer to a centrifuge and centrifuge at 1300r / min for 15 minutes, and take the supernatant after the end. Wash the residue after centrifugation once with a small amount of 50wt% ethanol aqueous solution, mix the washing liquid and the supernatant, heat to remove the ethanol, and obtain a water extract. Extract the water extract with petroleum ether, and after extraction, put the aqueous layer solution into a heated water bath for concentration, and then dry it in an oven at 72°C to obtain the grape seed extract, which is refrigerated at 0-4°C for use.

[0042] Preparation of oat extract: grind oats through a 100-mesh sieve, weigh 30 kg and dissolve in 80 wt% ethanol aqueous solution, stir and extract at 69°C for 2 hours, then transfer to a centrifuge and centrifuge at 4200 r / min for 15 minutes, and take the supernatant after the end. Wash the residue after centrifugation once with a small amount of 80 wt% ethanol aqueous solution, mix the washing liquid and the supernatant, filter under negative pressure, and then concentrate by rotary evaporation at 59°C to constant weight to obtain the oat extract, which is refrigerated at 0-4°C for later use.

[0043] Preparation of pine pollen extract: 30 kg of pine pollen is weighed through a 200-mesh sieve, and pine pollen and water are added at a solid-liquid ratio of 1:27, and ultrasonic reflux extraction is performed at 48.5°C, 40W, and 40KHz for 2 hours, and the residue after ultrasonic reflux is subjected to secondary extraction, and the filtrates obtained twice are collected; the filtrate is concentrated under reduced pressure at 59°C to an extract, and a 75wt% ethanol aqueous solution with a mass of 3.5 times that of the concentrated solution is added, stirred for 10 minutes, and allowed to stand for alcohol precipitation for 12 hours, and the alcohol precipitation liquid is filtered, and the obtained filtrate is concentrated under reduced pressure at 60°C to an extract; and a 95wt% ethanol aqueous solution with a mass of 3.5 times that of the concentrated solution is added, stirred for 10 minutes, and allowed to stand for alcohol precipitation for 12 hours, and the alcohol precipitation liquid is filtered, and the obtained filter residue is sprayed with anhydrous ethanol, and then freeze-dried and ground into powder to obtain the pine pollen extract.

[0044] 1.2 kg of soybean extract, 0.7 kg of glabridin, 1.7 kg of maitake polysaccharide, 1.2 kg of grape seed extract, 0.7 kg of oat extract, 0.7 kg of pine pollen extract, and 0.3 kg of tea tree oil were weighed respectively, mixed, and then mixed with ceramide at a mass ratio of 1:12 to obtain a mixture, the mixture was stirred at high speed for 30 min at high temperature with a magnetic stirrer, then ultrasonicated for 30 min, and then centrifuged at 10500 r / min for 30 min, and the precipitate was washed 3 times with anhydrous ethanol to obtain prefabricated microspheres;

[0045] Add 0.85 L of 5.0% sodium dodecyl sulfate solution to 2 kg of prefabricated microspheres, stir in a constant temperature water bath at 30°C until uniform, continue to add 1.9 L of 3.0% sodium alginate solution, and stir at high speed; then add 3.9 L of 3.0% chitosan solution, continue stirring for 30 minutes to prepare a microcapsule solution, take 1.9 L of 2.0% glutaraldehyde solution and slowly add it dropwise to the microcapsule solution, let it stand for 24 hours, filter and dry, and thus prepare a composition containing encapsulated sustained-release anti-dandruff and antipruritic effects.

[0046] Example 3

[0047] A composition with slow-release anti-dandruff and antipruritic effects is prepared by the following specific steps:

[0048] Preparation of grape seed extract: crush the grape seeds through an 80-mesh sieve, weigh 50 kg and dissolve them in a 50wt% ethanol aqueous solution, extract at 23°C for 2h, then transfer to a centrifuge and centrifuge at 1350r / min for 15min, and take the supernatant after the end. Wash the residue after centrifugation twice with a small amount of 50wt% ethanol aqueous solution, mix the washing liquid and the supernatant, heat to remove the ethanol, and obtain a water extract. Extract the water extract with petroleum ether, and after extraction, put the aqueous layer solution into a heated water bath for concentration, and then dry it in an oven at 73°C to obtain the grape seed extract, which is refrigerated at 0-4°C for use.

[0049] Preparation of oat extract: grind oats through a 100-mesh sieve, weigh 30 kg and dissolve in 80 wt% ethanol aqueous solution, stir and extract at 70°C for 2 hours, then transfer to a centrifuge and centrifuge at 4300 r / min for 15 minutes, and take the supernatant after the end. Wash the residue after centrifugation twice with a small amount of 80 wt% ethanol aqueous solution, mix the washing liquid and the supernatant, filter under negative pressure, and concentrate by rotary evaporation at 60°C to constant weight to obtain the oat extract, which is refrigerated at 0-4°C for later use.

[0050] Preparation of pine pollen extract: 30 kg of pine pollen is weighed through a 200-mesh sieve, and pine pollen and water are added at a solid-liquid ratio of 1:28, and ultrasonic reflux extraction is performed at 49° C., 40 W, and 40 kHz for 2 hours, and the residue after ultrasonic reflux is subjected to secondary extraction, and the filtrates obtained twice are collected; the filtrate is concentrated under reduced pressure at 60° C. to an extract, and a 75wt% ethanol aqueous solution with a mass of 4 times that of the concentrated solution is added, stirred for 10 minutes, and allowed to stand for alcohol precipitation for 12 hours, and the alcohol precipitation liquid is filtered, and the obtained filtrate is concentrated under reduced pressure at 60° C. to an extract; and a 95wt% ethanol aqueous solution with a mass of 4 times that of the concentrated solution is added, stirred for 10 minutes, and allowed to stand for alcohol precipitation for 12 hours, and the alcohol precipitation liquid is filtered, and the obtained filtrate is sprayed with anhydrous ethanol on the obtained filter residue, and then freeze-dried and ground into powder to obtain the pine pollen extract.

[0051] 1.4 kg of soybean extract, 0.9 kg of glabridin, 1.8 kg of maitake polysaccharide, 1.3 kg of grape seed extract, 0.8 kg of oat extract, 0.8 kg of pine pollen extract, and 0.4 kg of tea tree oil were weighed respectively, mixed, and then mixed with ceramide at a mass ratio of 1:1.1 to obtain a mixture, the mixture was stirred at high speed for 30 min at high temperature with a magnetic stirrer, then ultrasonicated for 30 min, and then centrifuged at 11000 r / min for 30 min, and the precipitate was washed 3 times with anhydrous ethanol to obtain prefabricated microspheres;

[0052] Add 0.9 L of 5.0% sodium dodecyl sulfate solution to 2 kg of prefabricated microspheres, stir in a constant temperature water bath at 30°C until uniform, continue to add 2.0 L of 3.0% sodium alginate solution, and stir at high speed; then add 4.0 L of 3.0% chitosan solution, continue stirring for 30 minutes to prepare a microcapsule solution, take 2.0 L of 2.0% glutaraldehyde solution and slowly add it dropwise to the microcapsule solution, let it stand for 24 hours, filter and dry, and thus obtain a composition containing encapsulated sustained-release anti-dandruff and antipruritic effects.

[0053] Example 4

[0054] A composition with slow-release anti-dandruff and antipruritic effects is prepared by the following specific steps:

[0055] Preparation of grape seed extract: crush the grape seeds through an 80-mesh sieve, weigh 50 kg and dissolve them in a 50wt% ethanol aqueous solution, extract at 24°C for 2 hours, then transfer to a centrifuge and centrifuge at 1450r / min for 15 minutes, and take the supernatant after the end. Wash the residue after centrifugation three times with a small amount of 50wt% ethanol aqueous solution, mix the washing liquid and the supernatant, heat to remove the ethanol, and obtain a water extract. Extract the water extract with petroleum ether, and after extraction, put the aqueous layer solution into a heated water bath for concentration, and then dry it in an oven at 74°C to obtain the grape seed extract, which is refrigerated at 0-4°C for use.

[0056] Preparation of oat extract: grind oats through a 100-mesh sieve, weigh 30 kg and dissolve in 80 wt% ethanol aqueous solution, stir and extract at 68-72°C for 2 hours, then transfer to a centrifuge and centrifuge at 4400 r / min for 15 minutes, and take the supernatant after the end. Wash the residue after centrifugation three times with a small amount of 80 wt% ethanol aqueous solution, mix the washing liquid and the supernatant, filter under negative pressure, and then concentrate by rotary evaporation at 61°C to constant weight to obtain the oat extract, which is refrigerated at 0-4°C for later use.

[0057] Preparation of pine pollen extract: 30 kg of pine pollen is weighed through a 200-mesh sieve, and pine pollen and water are added at a solid-liquid ratio of 1:29, and ultrasonic reflux extraction is performed at 49.5° C., 40 W, and 40 kHz for 2 hours, and the residue after ultrasonic reflux is subjected to secondary extraction, and the filtrates obtained twice are collected; the filtrate is concentrated under reduced pressure at 61° C. to an extract, and a 75wt% ethanol aqueous solution with a mass of 4.5 times that of the concentrate is added, stirred for 10 minutes, and allowed to stand for alcohol precipitation for 12 hours, and the alcohol precipitation liquid is filtered, and the obtained filtrate is concentrated under reduced pressure at 60° C. to an extract; and a 95wt% ethanol aqueous solution with a mass of 4.5 times that of the concentrate is added, stirred for 10 minutes, and allowed to stand for alcohol precipitation for 12 hours, and the alcohol precipitation liquid is filtered, and the obtained filter residue is sprayed with anhydrous ethanol, and then freeze-dried and ground into powder to obtain the pine pollen extract.

[0058] 1.4 kg of soybean extract, 0.9 kg of glabridin, 1.9 kg of maitake polysaccharide, 1.4 kg of grape seed extract, 0.9 kg of oat extract, 0.9 kg of pine pollen extract, and 0.4 kg of tea tree oil were weighed respectively, mixed, and then mixed with ceramide at a mass ratio of 1:15 to obtain a mixture, the mixture was stirred at high speed for 30 min at high temperature with a magnetic stirrer, then ultrasonicated for 30 min, and then centrifuged at 11500 r / min for 30 min, and the precipitate was washed 3 times with anhydrous ethanol to obtain prefabricated microspheres;

[0059] Add 0.95 L of 5.0% sodium dodecyl sulfate solution to 2 kg of prefabricated microspheres, stir in a constant temperature water bath at 30°C until uniform, continue to add 2.1 L of 3.0% sodium alginate solution, and stir at high speed; then add 4.1 L of 3.0% chitosan solution, continue stirring for 30 minutes to prepare a microcapsule solution, take 2.1 L of 2.0% glutaraldehyde solution and slowly add it dropwise to the microcapsule solution, let it stand for 24 hours, filter and dry, and thus obtain a composition containing encapsulated sustained-release anti-dandruff and antipruritic effects.

[0060] Example 5

[0061] A composition with slow-release anti-dandruff and antipruritic effects is prepared by the following specific steps:

[0062] Preparation of grape seed extract: crush the grape seeds through an 80-mesh sieve, weigh 50 kg and dissolve them in a 50wt% ethanol aqueous solution, extract at 25°C for 2 hours, then transfer to a centrifuge and centrifuge at 1500r / min for 15 minutes, and take the supernatant after the end. Wash the residue after centrifugation three times with a small amount of 50wt% ethanol aqueous solution, mix the washing liquid and the supernatant, heat to remove the ethanol, and obtain a water extract. Extract the water extract with petroleum ether, and after extraction, put the aqueous layer solution into a heated water bath for concentration, and then dry it in an oven at 75°C to obtain the grape seed extract, which is refrigerated at 0-4°C for use.

[0063] Preparation of oat extract: grind oats through a 100-mesh sieve, weigh 30 kg and dissolve in 80 wt% ethanol aqueous solution, stir and extract at 72°C for 2 hours, then transfer to a centrifuge and centrifuge at 4500 r / min for 15 minutes, and take the supernatant after the end. Wash the residue after centrifugation three times with a small amount of 80 wt% ethanol aqueous solution, mix the washing liquid and the supernatant, filter under negative pressure, and then concentrate by rotary evaporation at 62°C to constant weight to obtain the oat extract, which is refrigerated at 0-4°C for later use.

[0064] Preparation of pine pollen extract: 30 kg of pine pollen is weighed through a 200-mesh sieve, and pine pollen and water are added at a solid-liquid ratio of 1:30, and ultrasonic reflux extraction is performed at 50° C., 40 W, and 40 kHz for 2 hours, and the residue after ultrasonic reflux is subjected to secondary extraction, and the filtrates obtained twice are collected; the filtrate is concentrated under reduced pressure at 62° C. to an extract, and a 75wt% ethanol aqueous solution with a mass of 5 times that of the concentrated solution is added, stirred for 10 minutes, and allowed to stand for alcohol precipitation for 12 hours, and the alcohol precipitation liquid is filtered, and the obtained filtrate is concentrated under reduced pressure at 60° C. to an extract; and a 95wt% ethanol aqueous solution with a mass of 5 times that of the concentrated solution is added, stirred for 10 minutes, and allowed to stand for alcohol precipitation for 12 hours, and the alcohol precipitation liquid is filtered, and the obtained filter residue is sprayed with anhydrous ethanol, and then freeze-dried and ground into powder to obtain the pine pollen extract.

[0065] 1.5 kg of soybean extract, 1 kg of glabridin, 2 kg of maitake polysaccharide, 1.5 kg of grape seed extract, 1 kg of oat extract, 1 kg of pine pollen extract, and 0.5 kg of tea tree oil were weighed respectively, mixed, and then mixed with ceramide at a mass ratio of 1:1.2 to obtain a mixture, the mixture was stirred at high speed for 30 min at high temperature with a magnetic stirrer, then ultrasonicated for 30 min, and then centrifuged at 12000 r / min for 30 min, and the precipitate was washed 3 times with anhydrous ethanol to obtain prefabricated microspheres;

[0066] Add 1 L of 5.0% sodium dodecyl sulfate solution to 2 kg of prefabricated microspheres, stir in a constant temperature water bath at 30°C until uniform, continue to add 2.2 L of 3.0% sodium alginate solution, and stir at high speed; then add 4.2 L of 3.0% chitosan solution, continue stirring for 30 minutes to prepare a microcapsule solution, take 2.2 L of 2.0% glutaraldehyde solution and slowly add it dropwise to the microcapsule solution, let it stand for 24 hours, filter and dry, and thus obtain a composition containing encapsulated sustained-release anti-dandruff and antipruritic effects.

[0067] Comparative Example 1

[0068] A composition with sustained-release anti-dandruff and antipruritic effects was prepared. The difference from Example 3 was that pine pollen extract was not added to the composition, but an equal mass of soybean extract was used instead. The rest was the same as Example 3.

[0069] Comparative Example 2

[0070] A composition with sustained-release anti-dandruff and antipruritic effects was prepared. The difference from Example 3 was that oat extract was not added to the composition, but an equal mass of soybean extract was used instead. The rest was the same as Example 3.

[0071] Comparative Example 3

[0072] A composition with sustained-release anti-dandruff and antipruritic effects was prepared. The difference from Example 3 was that Grifola frondosa polysaccharide was not added to the composition, but grape seed extract of equal mass was used instead. The rest was the same as Example 3.

[0073] Comparative Example 4

[0074] A composition with sustained-release anti-dandruff and antipruritic effects was prepared. The difference from Example 3 was that the composition was only coated with ceramide, without chitosan or sodium alginate, and without adding glutaraldehyde. The rest was the same as Example 3.

[0075] Comparative Example 5

[0076] A composition with sustained-release anti-dandruff and antipruritic effects was prepared. The difference from Example 3 was that glutaraldehyde was not added during the preparation of the composition, and the rest was the same as Example 3.

[0077] The following tests were conducted on the compositions containing the slow-release anti-dandruff and antipruritic effects prepared in Examples 1 to 5 and Comparative Examples 1 to 5:

[0078] Test Example 1 Antibacterial Effect Test

[0079] According to Appendix C4 "Test method for antibacterial performance of dissolution antibacterial (inhibitory) products" in GB 1 5797-2002 "Hygiene standard for disposable sanitary products", the inhibitory ability of the product against Malassezia was tested and the antibacterial rate of Malassezia furfur was calculated. The calculation formula is as follows. The results are shown in Table 1.

[0080]

[0081] Where:

[0082] A-average colony count of control samples;

[0083] B-average colony count of the tested samples;

[0084] Table 1

[0085]

[0086]

[0087] As can be seen from Table 1, the compositions containing sustained-release anti-dandruff and antipruritic effects obtained in Examples 1 to 5 of the present invention and Comparative Examples 1 to 5 have good Malassezia furfur inhibition rates, all reaching more than 94%. Comparative Examples 1 to 3 also have good antibacterial effects, but the antibacterial effects of Comparative Examples 4 and 5 are significantly lower than those of Example 3. It can be seen that the presence of glutaraldehyde has a significant effect on the Malassezia furfur inhibition rate of the composition.

[0088] The compositions containing the slow-release anti-dandruff and antipruritic effects prepared in Examples 1 to 5 and Comparative Examples 1 to 5 were used as effective ingredients to prepare scalp care solutions according to the raw materials in Table 2, and their barrier functions were tested.

[0089] Table 2

[0090]

[0091] Test Example 2 Barrier Function Test

[0092] (1) Selection of subjects

[0093] Thirty healthy subjects were selected, and the inclusion criteria were as follows: ① healthy subjects aged between 18 and 60 years old (excluding pregnant or lactating women); ② those with problems such as oily hair, hair loss, unstable scalp, sensitive scalp and damaged hair (such as broken hair / split ends); ③ no obvious skin lesions or scars on the scalp. Exclusion criteria: ① Patients who have used antihistamines in the past week or immunosuppressants in the past month; ② Patients with a history of skin diseases such as psoriasis, eczema, atopic dermatitis, severe acne, or other conditions that may affect the results of the experiment; ③ Patients with severe androgenic alopecia, alopecia areata, inflammatory cicatricial alopecia, or other scalp / hair diseases; ④ Patients with allergic diseases, allergies to cosmetics or other topical preparations; ⑤ Patients who have received hair transplantation treatment; ⑥ Patients with insulin-dependent diabetes; ⑦ Patients with asthma or other chronic respiratory diseases being treated; ⑧ Patients who have received anticancer chemotherapy in the past six months; ⑨ Patients with immunodeficiency or autologous diseases; ⑩ Women who are breastfeeding or pregnant, or those who are planning to get pregnant in the near future; Those who are currently participating in or have participated in other clinical trials within the past two months; People with highly sensitive constitutions. This study strictly complies with the Helsinki Declaration of the National Medical Association, and all subjects understand the purpose and process of the entire experiment and sign the informed consent.

[0094] (2) Test method

[0095] The test personnel screened the subjects according to the subject inclusion and exclusion criteria, and randomly divided them into 10 groups of 3 people each. The scalp care solutions prepared in Examples 1 to 5 and Comparative Examples 1 to 5 were applied respectively. The subjects who met the requirements used the products for 4 weeks in accordance with the product use requirements. Before using the product (D0) and 28 days after using the product (D28), the subjects sat quietly in a constant temperature and humidity environment (20°C to 22°C, relative humidity 40% to 60%) for at least 30 minutes, and the test personnel conducted instrument tests and collected relevant parameters. The test of the same subject must be completed by the same tester using the same instrument, and the test probe should be cleaned between two measurements.

[0096] (3) Requirements for subjects

[0097] ① Subjects were asked to use the product according to the product instructions, once a day, and wash their hair the night before each visit.

[0098] ②During the test, subjects need to maintain good work and rest habits and avoid staying up late.

[0099] (4) Test items

[0100] ① Transepidermal water loss rate

[0101] The test was performed using an ultra-small TEWL test probe. The smaller the measured value, the less transepidermal water loss per unit time and per unit cross-sectional area, and the better the skin barrier. The test results were averaged, and the results are shown in Table 3.

[0102] ② Water content of stratum corneum

[0103] The test was conducted using the Moisture with pin probe from the Danish company Cortex. The test principle is based on the conductivity method to measure the moisture content of the skin stratum corneum. The larger the measured value, the higher the moisture content of the scalp. The test results were averaged, and the results are shown in Table 3.

[0104] Table 3

[0105]

[0106] As shown in Table 3, the scalp care solutions with slow-release anti-dandruff and antipruritic effects prepared in Examples 1 to 5 of the present invention showed lower transepidermal water loss and higher stratum corneum moisture content after 28 days of use, which comprehensively reflects the good barrier function of the scalp. However, the scalp care solutions with slow-release anti-dandruff and antipruritic effects prepared in Comparative Examples 1 to 5 showed an increase in transepidermal water loss and a decrease in stratum corneum moisture content after 28 days of use, indicating that their barrier function is inferior to that of the scalp care solutions with slow-release anti-dandruff and antipruritic effects prepared in Examples 1 to 5.

[0107] The above is only an implementation method of the invention, and does not limit the scope of patent protection. Those skilled in the art can make non-substantial changes or substitutions based on the invention, which still fall within the scope of patent protection.

Claims

1. A composition containing a sustained-release anti-dandruff and antipruritic effect, characterized in that: The raw materials of the composition include the following components in parts by weight: 10-15 parts of soybean extract Glabridin 5-10 parts 15-20 parts of Grifola frondosa polysaccharide Grape seed extract 10-15 parts 5-10 parts oat extract 5-10 parts of pine pollen extract 2-5 parts of tea tree oil; The grape seed extract is obtained by extraction with a 50wt% ethanol aqueous solution at 20-25°C; The oat extract is prepared by extraction with 80wt% ethanol aqueous solution at 68-72°C; The pine pollen extract is prepared by water extraction and alcohol precipitation, wherein the solid-liquid ratio is 1:25-30 and the temperature is 48-50° C. during the water extraction process; and 3-5 times of 75wt% ethanol aqueous solution is used during the alcohol precipitation process; The composition is prepared by a preparation method comprising the following steps: Preparing prefabricated microspheres: mixing the raw materials of the composition with ceramide to obtain a mixture, stirring and ultrasonicating the mixture, and then centrifuging to obtain prefabricated microspheres; Preparation of the composition: The prefabricated microspheres are sequentially mixed with sodium dodecyl sulfate, sodium alginate, chitosan and glutaraldehyde, and allowed to stand to obtain the composition.

2. The composition according to claim 1, characterized in that In the step of preparing prefabricated microspheres in the preparation method, the mass ratio of the raw material to ceramide is 1:1 to 1.

2.

3. The composition according to claim 2, characterized in that In the preparation step of the preparation method, sodium dodecyl sulfate, sodium alginate, chitosan and glutaraldehyde are added in the form of sodium dodecyl sulfate solution, sodium alginate solution, chitosan solution and glutaraldehyde solution with mass concentrations of 5.0%, 3.0%, 3.0% and 2.0% respectively.

4. The composition according to claim 3, characterized in that The dosage ratio of the prefabricated microspheres to the sodium dodecyl sulfate solution, the sodium alginate solution, the chitosan solution and the glutaraldehyde solution is 2kg: 0.8-1mL: 1.8-2.2mL: 3.8-4.2mL: 1.8-2.2mL.

5. Use of the composition according to any one of claims 1 to 4 as an effective ingredient in the preparation of anti-dandruff and antipruritic products.

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