A composition and its application in color enhancement of red-leafed photinia

Abstract

The present invention relates to the field of red-leafed photinia cultivation technology, and discloses a composition and its use in enhancing the color of red-leafed photinia. The composition, when sprayed on the leaves, comprises 3.375-6.75 g / mu of chlorpyrifos methyl, 150-300 g / mu of mineral oil, 0.3-0.6 g / mu of iron, 0.3-0.6 g / mu of zinc, 0.007-0.014 g / mu of copper, and 0.01-0.02 g / mu of molybdenum. The present invention compounds chlorpyrifos methyl, mineral oil, and trace elements (iron, zinc, copper, and molybdenum) into a composition. The active ingredients in the composition complement each other and act synergistically to promote the color enhancement and reddening of red-leafed photinia leaves. The red-leafed photinia color enhancement method provided by the present invention is safe, easy to perform, and simple to operate. Only one spray per year is required to effectively enhance the color of red-leafed photinia leaves, thereby enhancing the ornamental value of red-leafed photinia. The method is suitable for nationwide promotion and application.

Description

Technical Field

[0001] The invention relates to the technical field of red-leafed photinia cultivation, and in particular to a composition and application thereof in enhancing the color of red-leafed photinia. Background Art

[0002] Red-leafed Photinia (Photinia × fraseri Dress) is a hybrid of the genus Photinia in the Rosaceae family. It is a small evergreen tree or shrub, reaching up to 5 meters in height for trees and 2 meters for shrubs. It is primarily distributed in subtropical and temperate regions of southeastern and eastern Asia and North America, and is also widely cultivated in many provinces of China. As a street tree, its upright stems resemble torches; as a hedge, its form resembles a crouching fire dragon. When pruned and landscaped, it can be shaped in a variety of ways, creating a beautiful landscape effect. Red-leafed Photinia derives its name from the bright red color of its new shoots and young leaves.

[0003] However, due to the differences in cultivation areas, the influence of soil and climate (light and temperature), the brightness of the color of the leaves of Photinia fraseri will also be affected, thus affecting the ornamental value of Photinia fraseri. Summary of the Invention

[0004] In view of this, the present invention provides a composition and its application in color enhancement of Photinia fraseri, which can significantly increase the anthocyanin content of Photinia fraseri leaves, make the color of Photinia fraseri more vivid, and enhance the ornamental value of Photinia fraseri.

[0005] To achieve the above objectives, the technical solutions adopted by the present invention are as follows.

[0006] In the first aspect, the present invention provides an application of a composition in enhancing the color of photinia fraseri, and the application provides a method for enhancing the color of photinia fraseri, which comprises using the composition to treat photinia fraseri, and the treatment method is foliar spraying, and the foliar spraying amount of the composition is 3.375-6.75 g / mu of clopyralid / methyl, 150-300 g / mu of mineral oil, 0.3-0.6 g / mu of iron, 0.3-0.6 g / mu of zinc, 0.0007-0.014 g / mu of copper, and 0.01-0.02 g / mu of molybdenum.

[0007] Furthermore, the composition is used to treat Photinia fraseri once a year.

[0008] In the above composition, clopyralid is a selective systemic herbicide. After foliar spraying, it inhibits the ALS enzyme acetolactate synthase of Photinia fraseri, prevents the biosynthesis of branched-chain amino acids such as valine, isoleucine, and leucine, destroys protein synthesis, interferes with DNA synthesis and cell division and growth, affects the synthesis of anthocyanins in Photinia fraseri leaves, and ultimately affects the leaf color of Photinia fraseri leaves.

[0009] The trace element iron is a component of cytochromes and non-heme iron proteins involved in photosynthesis, biological nitrogen fixation, and respiration, and is essential for chlorophyll synthesis. Since iron is required for the synthesis of certain chlorophyll-protein complexes in chloroplasts, iron deficiency hinders and disrupts chlorophyll formation, leading to a lack of green between leaf veins. Because iron is not easily transferred from older leaves, iron deficiency often occurs in young leaves. When iron deficiency is severe or prolonged, the veins also lack green, and the entire leaf may become bleached.

[0010] The trace element zinc is a component of enzymes such as alcohol dehydrogenase, glutamate dehydrogenase, and carbonic anhydrase. Zinc-deficient plants lose the ability to synthesize tryptophan, a precursor to indoleacetic acid, resulting in low indoleacetic acid levels. Zinc is an essential element for chlorophyll-producing plants. Zinc deficiency can result in short internodes on the stem, a lotus-like appearance, small, deformed leaves, and a lack of green.

[0011] The trace element copper is a component of certain oxidases (such as ascorbate oxidase and tyrosinase), affecting redox processes. Copper is also present in plastocyanin in chloroplasts, a component of the photosynthetic electron transport system. Copper deficiency can cause leaves to become dark green with necrosis, starting at the tips of young leaves and spreading along the margins to the base. Leaves may also become wrinkled or deformed. Extreme copper deficiency can cause leaf drop.

[0012] Molybdenum ions are the metallic component of nitrate reductase, playing a role in electron transfer. Molybdenum is also a component of the molybdenum-iron protein in nitrogenase, playing a role in nitrogen fixation. Molybdenum deficiency can cause interveinal chlorosis and necrosis in older leaves.

[0013] The addition of mineral oil can enhance the absorption of clopyrazosulfuron-methyl and trace elements, making the absorption and utilization rate of clopyrazosulfuron-methyl and trace elements higher and the effect better.

[0014] Finally, clopyrazosulfuron-methyl, mineral oil and trace elements (iron, zinc, copper, molybdenum) in the above composition complement each other and work synergistically to jointly promote the color enhancement and reddening of the leaves of Photinia fraseri.

[0015] In some embodiments, the composition can be prepared as a pesticide formulation and then sprayed on the leaves. Preparing the composition as a pesticide formulation can enhance the dispersibility of the active ingredients in the composition and improve the effect of the composition.

[0016] Preferably, the dosage forms of the pesticide preparation include emulsifiable concentrates, microemulsions, aqueous emulsions, suspension concentrates, oil suspension concentrates, dry suspension concentrates, wettable powders and water dispersible granules.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] The present invention combines clopyrazosulfuron-methyl, mineral oil, and trace elements (iron, zinc, copper, and molybdenum) into a composition. The active ingredients in the composition complement each other and work synergistically to enhance the color and reddening of Photinia fraseri leaves. The color enhancement method provided by the present invention is safe, easy, and simple to operate. Only one spraying per year is required to effectively enhance the color of Photinia fraseri leaves, achieving the goal of enhancing the ornamental value of Photinia fraseri. The method is suitable for nationwide promotion and application. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 This is a comparative bar graph of anthocyanin content in Photinia fraseri after being treated with different concentrations of chlorpyrifos methyl;

[0020] Figure 2 This is a comparative bar graph of anthocyanin content in leaves of Photinia fraseri in Experiment 1 of the present invention;

[0021] Figure 3 This is a comparative bar graph of anthocyanin content in leaves of Photinia fraseri in Experiment 2 of the present invention;

[0022] Figure 4 This is a comparative bar chart of anthocyanin content in the leaves of Photinia fraseri in Experiment 3 of the present invention. DETAILED DESCRIPTION

[0023] The present invention will be further described below with reference to the accompanying drawings and specific examples. It should be understood that the specific examples and drawings are merely illustrative of the present invention and should not be construed as the entire invention or as a limitation or restriction of the technical solutions of the present invention. Unless otherwise specified, the raw materials used in the following examples and comparative examples can be obtained through conventional commercial channels.

[0024] In the following examples and comparative examples, the mass concentration of clopyrazosulfuron-methyl is 75%, the mass concentration of mineral oil is 98%, and the trace element aqueous solution is an aqueous solution formed by mixing 3 wt % of iron, 3 wt % of zinc, 0.07 wt % of copper and 0.1 wt % of molybdenum.

[0025] 1. Preparation of liquid composition for color enhancement test of Photinia fraseri Example

[0026] 3 g of clopyrazosulfuron-methyl (active ingredient 2.25 g), 100 g of mineral oil (active ingredient 98 g) and 20 g of aqueous trace element solution (active ingredients: iron 0.6 g, zinc 0.6 g, copper 0.014 g, molybdenum 0.02 g) were mixed evenly and diluted with water to 15 L to prepare composition liquid 1. Example

[0027] 2 g of clopyrazosulfuron-methyl (active ingredient 1.5 g), 67 g of mineral oil (active ingredient 65.66 g) and 13.3 g of trace element aqueous solution (active ingredients: iron 0.4 g, zinc 0.4 g, copper 0.0093 g, molybdenum 0.013 g) were mixed evenly and diluted with water to 15 L to prepare composition liquid 2. Example

[0028] 1.5 g of clopyrazosulfuron-methyl (active ingredient 1.125 g), 50 g of mineral oil (active ingredient 49 g) and 10 g of aqueous trace element solution (active ingredients: 0.3 g iron, 0.3 g zinc, 0.007 g copper, 0.01 g molybdenum) were mixed evenly and diluted with water to 15 L to prepare composition liquid 3.

[0029] Comparative Example 1:

[0030] The same method as Example 1 was used, except that chlorpyrifos methyl was not included, to prepare comparative solution 1.

[0031] Comparative Example 2:

[0032] The same method as Example 1, except that the mineral oil was not contained, was used to prepare comparative solution 2.

[0033] Comparative Example 3:

[0034] The same as Example 1, except that the trace element aqueous solution was not contained, to prepare comparative solution 3.

[0035] 2. Test on the phytotoxicity of chlorpyrifos on Photinia fraseri

[0036] Chlorosulfuron-methyl can inhibit the biosynthesis of branched-chain amino acids leucine, isoleucine, and valine in plants, but improper use may cause phytotoxicity to crops. Therefore, chlorpyrifos-methyl was used in a phytotoxicity test on Photinia fraseri.

[0037] Using normally grown Photinia fraseri as a control (CK), 75 wt% clopyralidosulfuron-methyl was diluted with water and sprayed onto the leaves at the rates shown in Table 1 below. Leaf growth was observed and, after 30 days, new and old leaves were collected for anthocyanin content testing. The anthocyanin content test method was as follows: the leaves to be tested were ground into a powder using liquid nitrogen and extracted with a 1% hydrochloric acid-methanol solution. The extraction was performed at 3000 rpm. -1 After centrifugation for 5 minutes, the supernatant was collected and the absorbance was measured at 530 nm and 657 nm, respectively. A standard curve for anthocyanins was drawn using 1% hydrochloric acid-methanol solution as a blank control, and the anthocyanin content was calculated. The test results are shown in Table 1 below.

[0038] Table 1. Effects of different concentrations of chlorpyrifos methyl on Photinia fraseri

[0039]

[0040] Note: The anthocyanin content data in Table 1 are all average values, the number of repetitions is 3, and the subsequent growth observation period of the leaves is 3 months.

[0041] Figure 1 This is a comparison of the anthocyanin content in Photinia fraseri after treatment with different concentrations of chlorpyrifos methyl.

[0042] From Table 1 and Figure 1 As can be seen from the results, only when the chlorpyrifos methyl dosage exceeds 3.375g / mu does a significant color-enhancing effect appear on red-leafed photinia. When the dosage is gradually increased to 6.75g / mu, the color-enhancing effect reaches its peak, and the leaves of the red-leafed photinia grow normally with no phytotoxicity. However, when the chlorpyrifos methyl dosage reaches 7.785g / mu, the red-leafed photinia fails to enhance color and is accompanied by phytotoxicity, such as curling and deforming of the leaves, which affects the landscape effect. When the chlorpyrifos methyl dosage reaches 9.0g / mu, the phytotoxicity worsens. Therefore, this experiment shows that the chlorpyrifos methyl dosage for color-enhancing on red-leafed photinia should be controlled between 3.375g and 6.75g / mu, with the optimal color-enhancing effect achieved at 6.75g / mu. Below this range, there is no color-enhancing effect, while above this range, severe phytotoxicity occurs and no color-enhancing effect is achieved.

[0043] 3. Color enhancement test of red-leafed photinia

[0044] The following color enhancement tests were conducted on Photinia fraseri using the liquid compositions prepared in the above examples and comparative examples. The tests were conducted on the same day in the same Photinia fraseri planting plot. The plot was divided into a treatment area and a control area. The treatment area was sprayed with the liquid composition on the leaves, while the control area was sprayed with water as a control. The spray rate for both the treatment and control areas was 45 L / mu. The spray rate of the liquid composition was determined based on the control range of the color enhancement dosage of clopyralidosulfuron-methyl on Photinia fraseri (3.375 g to 6.75 g / mu) combined with the clopyralidosulfuron-methyl content in each liquid composition.

[0045] Thirty days after treatment, five leaves, both new and old, were collected from the treated and control areas, repeated three times. The leaves were bagged, labeled, and sent for testing of anthocyanin content. The test results are shown in Table 2 below.

[0046] Table 2. Comparison of anthocyanin content in different test leaves

[0047]

[0048] Figure 2 A bar graph comparing the anthocyanin content of leaves of Photinia fraseri after being treated with the composition liquid 1 prepared in Example 1 and the anthocyanin content of leaves of Photinia fraseri after being treated with clean water; Figure 3This is a bar graph comparing the anthocyanin content of the leaves of Photinia fraseri after being treated with the composition liquid 2 prepared in Example 2 and the anthocyanin content of the leaves of Photinia fraseri treated with clear water; Figure 4 This is a bar graph comparing the anthocyanin content of the leaves of Photinia fraseri after being treated with the composition liquid 3 prepared in Example 3 and the anthocyanin content of the leaves of Photinia fraseri after being treated with clean water.

[0049] See Table 2 and Figures 2 to 4 It can be seen that compared with the clear water control group, the anthocyanin content of the new leaves of red-leafed photinia treated with composition liquid 1 increased by 85.1%, and the anthocyanin content of the old leaves increased by 43.3%; compared with the clear water control group, the anthocyanin content of the new leaves of red-leafed photinia treated with composition liquid 2 increased by 57.5%, and the anthocyanin content of the old leaves increased by 35.1%; compared with the clear water control group, the anthocyanin content of the new leaves of red-leafed photinia treated with composition liquid 3 increased by 59.7%, and the anthocyanin content of the old leaves increased by 46.4%; This shows that the composition liquids prepared in Examples 1 to 3 can significantly increase the anthocyanin content of the new and old leaves of red-leafed photinia after use, make the color of red-leafed photinia more vivid, and enhance the ornamental value of red-leafed photinia.

[0050] Refer to Table 2, Test 4. Comparative liquid 1 does not contain clopyralidone, and the anthocyanin content of the drug-treated group is only slightly higher than that of the water control group, indicating that clopyralidone plays a key role in the color enhancement application of Photinia fraseri.

[0051] Referring to Table 2, Experiment 5, comparative liquid 2 does not contain mineral oil. Although the drug-treated group can improve the color of the red-leafed photinia leaves to a certain extent, there is still a considerable gap compared with the color-enhancing effect of Experiment 1, indicating that the use of mineral oil in the composition is beneficial to improving the color-enhancing effect of the red-leafed photinia leaves.

[0052] Referring to Table 2, Experiment 6, comparative liquid 3 does not contain trace element aqueous solution. Although the drug treatment group can improve the color of the red-leafed photinia leaves to a certain extent, there is still a big gap compared with the color-enhancing effect of Experiment 1, indicating that the use of trace elements iron, zinc, copper, and molybdenum in the composition is beneficial to improving the color-enhancing effect of the red-leafed photinia leaves.

[0053] The above descriptions are merely embodiments of the present invention and are not intended to limit the patent scope of the present invention. Any equivalent structure or equivalent process transformation made using the contents of the present invention's description and drawings, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present invention.

Claims

1. A composition for enhancing the color of Photinia fraseri, characterized in that: The composition is used to treat Photinia fraseri by foliar spraying, and the spraying amount of the composition for foliar spraying is 3.375-6.75 g / mu of clopyralid, 150-300 g / mu of mineral oil, 0.3-0.6 g / mu of iron, 0.3-0.6 g / mu of zinc, 0.007-0.014 g / mu of copper, and 0.01-0.02 g / mu of molybdenum.

2. The use according to claim 1, characterized in that: The composition is used to treat Photinia fraseri once a year.

3. The use according to claim 1, characterized in that: The composition is prepared into a pesticide formulation and then sprayed on leaves.

4. The use according to claim 3, characterized in that: The dosage forms of the pesticide preparation include emulsifiable concentrate, microemulsion, aqueous emulsion, suspension concentrate, oil suspension concentrate, dry suspension concentrate, wettable powder and water dispersible granules.

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