Acne-removing and recurrence-preventing composition suitable for acne-sensitive skin and its application

Abstract

The present disclosure provides an acne-removing and recurrence-preventing composition suitable for acne-sensitive skin and its application, belonging to the field of cosmetic technology. The present disclosure selects epigallocatechin gallate glucoside, brazilin, Hibiscus sabdariffa flower extract, Ziziphus jojoba bark extract, linseed oil unsaponifiables, and guaiac extract as active ingredients of the acne-removing and recurrence-preventing composition. The active ingredients interact with each other to quickly inhibit the growth of acne-related pathogens and regulate the skin microecology; effectively inhibit skin inflammation throughout the acne process, play a role in preventing and suppressing inflammation, effectively repair the skin barrier, reduce redness, and regulate the skin's oil balance to prevent acne recurrence; in addition, the acne-removing and recurrence-preventing composition also has the effect of removing yellowness, evens skin tone, and improves skin roughness. The acne-removing and recurrence-preventing composition is mild and non-irritating and can be used on sensitive skin, expanding the application range of the acne-removing and recurrence-preventing composition.

Description

Technical Field

[0001] The present disclosure relates to the technical field of cosmetics, and in particular to an acne-removing and recurrence-preventing composition suitable for acne-sensitive skin and its application. Background Art

[0002] Acne is a common, chronic inflammatory skin disease of the hair follicles and sebaceous glands, primarily occurring in seborrheic areas such as the face, chest, and back. Acne is chronic and recurrent, potentially leading to infection, hyperpigmentation, and scarring. It can even have a serious negative impact on patients' quality of life and psychosocial functioning. The pathogenesis of acne is primarily related to increased sebum secretion, hyperkeratinization of the hair follicles and sebaceous ducts, Propionibacterium acnes, and inflammatory immunity.

[0003] Conventional acne treatments, including medications, physical therapy, and chemotherapy, all have serious side effects, such as bacterial resistance, disruption of the skin barrier, and inflammatory hyperpigmentation. Acne itself can also cause damage to the skin barrier. The use of skin care products as an adjunctive treatment for acne has garnered widespread attention. Due to their safety, good tolerability, and ability to improve a damaged skin barrier, most acne treatments on the market are slow to take effect and offer suboptimal results, failing to meet consumers' daily or special needs. Furthermore, short-acting acne treatments contain acids and sulfur, which stimulate a hyperimmune response in the skin, have a ripening effect, and are potentially irritating, making them unsuitable for those with sensitive skin. Importantly, most short-acting acne treatments are spot-on applications and are unsuitable for long-term maintenance and improvement of acne-prone skin. Furthermore, most products neglect post-acne care, significantly increasing the likelihood of recurrence and, over time, further damaging the skin barrier, leading to a vicious cycle. Summary of the Invention

[0004] The purpose of the present invention is to overcome the deficiencies of the prior art and provide an acne-removing and recurrence-preventing composition suitable for acne-sensitive skin and its application.

[0005] To achieve the above objectives, the technical solution adopted by the present invention is as follows: in the first aspect, a composition for removing acne and preventing recurrence suitable for acne-sensitive skin is provided, wherein the composition comprises the following components in parts by weight: 0.01-3 parts of epigallocatechin gallate glucoside, 0.001-0.3 parts of brazilian hematoxylin, 1-15 parts of Hibiscus sabdariffa flower extract, 0.1-1 parts of jujube bark extract, 0.01-3 parts of linseed oil unsaponifiable matter, and 1-15 parts of guaiac extract.

[0006] In some embodiments, the acne-recurrence prevention composition comprises the following components in parts by weight: 0.05-1 part of epigallocatechin gallate glucoside, 0.005-0.1 part of brazilian hematoxylin, 3-10 parts of Hibiscus sabdariffa flower extract, 0.1-0.5 part of jujube bark extract, 0.05-1 part of linseed oil unsaponifiable matter, and 1-8 parts of guaiac wood extract.

[0007] In some embodiments, the acne-recurrence prevention composition comprises the following components in parts by weight: 0.5-1 part of epigallocatechin gallate glucoside, 0.01-0.1 part of brazilian hematoxylin, 3-8 parts of Hibiscus sabdariffa flower extract, 0.15-0.5 part of jujube bark extract, 0.08-0.5 part of linseed oil unsaponifiable matter, and 2-5 parts of guaiac wood extract.

[0008] In some embodiments, the preparation method of the roselle flower extract comprises: adding roselle flowers to an acidified ethanol solution for ultrasonic extraction, performing solid-liquid separation after the extraction, and drying the obtained filtrate to obtain the roselle flower extract;

[0009] In some embodiments, the preparation method of the Zizyphus joazeiro bark extract is as follows: adding Zizyphus joazeiro bark to an ethanol aqueous solution for extraction, performing solid-liquid separation after the extraction, and drying the obtained filtrate to obtain the Zizyphus joazeiro bark extract.

[0010] In some embodiments, in the method for preparing the roselle flower extract, the water content of the roselle flower is 4-6%;

[0011] and / or, the average particle size of the roselle flowers is ≤150 μm;

[0012] and / or, the solid-to-liquid ratio of the roselle flower to the acidified ethanol solution is 1 g: 30-40 mL;

[0013] And / or, the frequency of the ultrasonic extraction is 120-160 Hz;

[0014] And / or, the ultrasonic extraction time is 25-35 min;

[0015] And / or, the temperature of the ultrasonic extraction is 45-55°C;

[0016] And / or, the pH of the acidified ethanol solution is 2.5-3.5.

[0017] In some embodiments, in the method for preparing the zizyphus joazeiro bark extract, the solid-to-liquid ratio of the zizyphus joazeiro bark to the ethanol aqueous solution is 1 g: 10-20 mL;

[0018] And / or, the volume percentage of ethanol in the ethanol aqueous solution is 65-75%;

[0019] And / or, the extraction method is one of leaching, reflux extraction, and ultrasonic extraction.

[0020] In a second aspect, the invention provides the use of the acne-removing and recurrence-preventing composition in the preparation of cosmetics.

[0021] In a third aspect, a cosmetic is provided, comprising the acne-eliminating and recurrence-preventing composition, wherein the content of the acne-eliminating and recurrence-preventing composition in the cosmetic is 0.1-3 wt %.

[0022] In some embodiments, the cosmetic is an anti-acne lotion, and the anti-acne lotion comprises the following components in percentage by weight: 0.1-3% of an anti-acne recurrence prevention composition, 0.05-5% of a thickener, 3-20% of a moisturizer, 0.5-10% of an emulsifier, 0.2-5% of a preservative, 0.05-0.1% of a pH adjuster, and the remainder being water.

[0023] In some embodiments, the thickener comprises at least one of xanthan gum, carbomer, ammonium acrylamide dimethyl taurate / VP copolymer, and cellulose;

[0024] And / or, the moisturizing agent includes at least one of glycerin, butylene glycol, and sodium hyaluronate;

[0025] And / or, the emulsifier includes at least one of coconut oil alcohol-caprylate / caprate, polydimethylsiloxane, hydrogenated palm kernel oil, cetearyl alcohol, polyglyceryl-6 distearate, and polymethylsilsesquioxane;

[0026] and / or, the preservative comprises at least one of 1,3-propylene glycol, 1,2-hexanediol, and p-hydroxyacetophenone;

[0027] And / or, the pH regulator includes at least one of arginine and disodium edetate.

[0028] Compared with the prior art, the present invention has the following beneficial effects: the present invention selects epigallocatechin gallate glucoside, brazilin, Hibiscus sabdariffa flower extract, jujube bark extract, linseed oil unsaponifiable matter and guaiac extract as active ingredients of the acne-removing and recurrence-preventing composition, which interact with each other to quickly inhibit the growth of acne-related pathogens and regulate the skin microecology; effectively inhibit skin inflammation throughout the entire process of acne, play a role in preventing and inhibiting inflammation, effectively repair the skin barrier, reduce redness, and at the same time have the effect of regulating the oil balance of the skin to prevent acne recurrence; in addition, the acne-removing and recurrence-preventing composition also has the effect of removing yellowness, evens skin tone, and improves skin roughness, and the acne-removing and recurrence-preventing composition is mild and non-irritating, and can be used for sensitive skin, thereby expanding the application range of acne-removing products. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1This is a graph showing the acne removal test results using the lotion from Application Example 1 on Days D0 and D7 in Effect Example 3;

[0030] Figure 2 This is a graph showing the acne removal test results on days D0 and D7 using the emulsion in comparative application example 1 in effect example 3. DETAILED DESCRIPTION

[0031] To facilitate understanding of the present disclosure, a more comprehensive description will be given below. However, the present disclosure can be implemented in many different forms and is not limited to the embodiments described herein. Rather, the purpose of providing these embodiments is to provide a more thorough and comprehensive understanding of the disclosure of the present disclosure.

[0032] As used herein:

[0033] "Prepared from" is synonymous with "comprising." As used herein, the terms "comprising," "including," "having," "containing," or any other variations thereof, are intended to cover a non-exclusive inclusion. For example, a composition, process, method, article, or apparatus that comprises the listed elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, process, method, article, or apparatus.

[0034] The conjunction "consisting of" excludes any unspecified element, step, or component. If used in a claim, this phrase renders the claim closed, excluding materials other than those described, except for conventional impurities associated therewith. When the phrase "consisting of" appears in a clause of the body of a claim, rather than immediately following the subject matter, it limits only the elements described in that clause; other elements are not excluded from the claim as a whole.

[0035] When amount, concentration or other value or parameter are represented with range, preferred range or the range that a series of upper preferred value and lower preferred value limit are expressed, this should be understood as specifically disclosing all ranges formed by any pairing of any range upper limit or preferred value and any range lower limit or preferred value, and no matter whether this scope is disclosed separately.For example, when disclosing scope " 1-5 ", described scope should be interpreted as including scope " 1-4 ", " 1-3 ", " 1-2 ", " 1-2 and 4-5 ", " 1-3 and 5 " etc.When numerical range is described in this article, unless otherwise stated, otherwise this scope is intended to include its end value and all integers and fractions within this range.

[0036] In these examples, parts and percentages are by mass unless otherwise indicated.

[0037] "Parts by mass" refers to the basic unit of measurement used to express the mass ratio of multiple components. One part can represent any unit of mass, such as 1g or 2.689g. If we say that the mass of component A is a parts and the mass of component B is b parts, this means the ratio of the mass of component A to the mass of component B is a:b. Alternatively, we could say that the mass of component A is aK and the mass of component B is bK (K is an arbitrary number representing a multiplication factor). It's important to note that, unlike parts by mass, the sum of the mass of all components is not limited to 100 parts.

[0038] "And / or" is used to indicate that one or both of the described situations may occur. For example, A and / or B includes (A and B) and (A or B). In a first aspect, a composition for removing acne and preventing recurrence suitable for acne-sensitive skin is provided, comprising the following components in parts by weight: 0.01-3 parts of epigallocatechin gallate glucoside, 0.001-0.3 parts of brazilian hematoxylin, 1-15 parts of Hibiscus sabdariffa flower extract, 0.1-1 parts of Zizyphus joazeiro bark extract, 0.01-3 parts of linseed oil unsaponifiables, and 1-15 parts of Guaiac extract.

[0039] Specifically, the weight proportion of the epigallocatechin gallate glucoside can be, but is not limited to, 0.01, 0.05, 0.1, 0.2, 0.5, 0.7, 1, 1.3, 1.5, 1.8, 2, 2.2, 2.5, 2.7, or 3 parts, preferably 0.05-1 part, and more preferably 0.5-1 part.

[0040] Specifically, the weight portion of the brazilin can be but is not limited to 0.001 part, 0.005 part, 0.01 part, 0.03 part, 0.05 part, 0.07 part, 0.1 part, 0.12 part, 0.15 part, 0.18 part, 0.2 part, 0.22 part, 0.25 part, 0.27 part, 0.3 part, preferably 0.005-0.1 part, more preferably 0.01-0.1 part.

[0041] Specifically, the weight proportion of the roselle flower extract can be, but is not limited to, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts, 8.5 parts, 9 parts, 9.5 parts, 10 parts, 10.5 parts, 11 parts, 11.5 parts, 12 parts, 12.5 parts, 13 parts, 13.5 parts, 14 parts, 14.5 parts, and 15 parts; preferably, 3-10 parts, and more preferably, 3-8 parts.

[0042] Specifically, the weight proportion of the zizyphus joazeiro bark extract can be, but is not limited to, 0.1 part, 0.15 part, 0.2 part, 0.25 part, 0.3 part, 0.35 part, 0.4 part, 0.45 part, 0.5 part, 0.55 part, 0.6 part, 0.65 part, 0.7 part, 0.75 part, 0.8 part, 0.85 part, 0.9 part, 0.95 part, or 1 part; preferably, 0.1-0.5 part, and more preferably, 0.15-0.5 part.

[0043] Specifically, the weight proportion of the unsaponifiable matter of linseed oil can be, but is not limited to, 0.01, 0.05, 0.1, 0.3, 0.5, 0.7, 1, 1.2, 1.5, 1.7, 2, 2.3, 2.5, 2.8, or 3 parts; preferably, 0.05-1 part, and more preferably, 0.08-0.5 part.

[0044] Specifically, the weight proportion of the guaiac extract can be, but is not limited to, 1 part, 1.2 parts, 1.5 parts, 1.7 parts, 2 parts, 2.3 parts, 2.5 parts, 2.8 parts, 3 parts, 3.5 parts, 4 parts, 4.3 parts, 4.5 parts, 4.8 parts, 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts, 9 parts, 10 parts, 11 parts, 11.5 parts, 12 parts, 13 parts, 14 parts, 14.5 parts, and 15 parts; preferably, 1-8 parts, and more preferably 2-5 parts.

[0045] The present invention selects epigallocatechin gallate glucoside, brazilin, Hibiscus sabdariffa flower extract, jujube bark extract, linseed oil unsaponifiables and guaiac extract as active ingredients of the acne-removing and recurrence-preventing composition, which interact with each other to quickly inhibit the growth of acne-related pathogens and regulate the skin microecology; effectively inhibit skin inflammation throughout the entire process of acne, play a role in preventing and inhibiting inflammation, effectively repair the skin barrier, reduce redness, and at the same time have the effect of regulating the oil balance of the skin to prevent acne recurrence; in addition, the acne-removing and recurrence-preventing composition also has the effect of removing yellowness, evens out skin color, and improves skin roughness, and the acne-removing and recurrence-preventing composition is mild and non-irritating, and can be used for sensitive skin, expanding the range of application of the acne-removing and recurrence-preventing composition.

[0046] Specifically, epigallocatechin gallate glucoside can effectively scavenge free radicals, slow down cell aging, protect cells from damage, has high anti-inflammatory properties, effectively improves acne and repairs skin; brazilian sappan wood can accelerate the improvement and healing of acne; roselle flower extract can protect the skin from external interference, and has antioxidant and antibacterial effects; jujube bark extract has anti-inflammatory, antimicrobial and immunomodulatory effects; linseed oil unsaponifiables mainly include linolenic acid, linolenic acid, linoleic acid, oleic acid, stearic acid and palmitic acid, which have anti-inflammatory and synergistic effects, and can also promote the metabolism of skin cells and enhance skin elasticity and radiance; guaiac wood extract is rich in lignin, quinones, saponins, sesquiterpenes and triterpenes, and has anti-inflammatory, antiviral, antibacterial, astringent and repairing effects.

[0047] Specifically, the content of each component in the acne-removing and recurrence-preventing composition will affect the overall performance of the acne-removing and recurrence-preventing composition. When the weight proportions of epigallocatechin gallate glucoside, brazilian sappan wood, roselle flower extract, jujube bark extract, linseed oil unsaponifiables and guaiac wood extract are within the preferred range, the obtained acne-removing and recurrence-preventing composition has better acne-removing, repairing and oil-control effects.

[0048] In some embodiments, the preparation method of the roselle flower extract comprises: adding roselle flowers to an acidified ethanol solution for ultrasonic extraction, performing solid-liquid separation after the extraction, and drying the obtained filtrate to obtain the roselle flower extract;

[0049] In the preparation method of the roselle flower extract, each parameter satisfies at least one of the following:

[0050] Specifically, the water content of the roselle flower is 4-6%; for example, it can be but not limited to 4%, 4.2%, 4.4%, 4.6%, 4.8%, 5%, 5.3%, 5.5%, 5.7%, 6%;

[0051] To obtain the aforementioned moisture content of the roselle flowers, the roselle flowers need to be dried. The present disclosure does not specify the drying method, as long as the moisture content of the roselle flowers reaches 4-6%, such methods include air drying, oven drying, and vacuum freeze drying. To preserve the active ingredient content in the roselle flowers, the present disclosure selects vacuum freeze drying.

[0052] Specifically, the drying steps of roselle flowers are as follows:

[0053] After pre-freezing the roselle flowers, the flowers are placed in a vacuum freeze drying oven at a vacuum degree of 80-100 Pa and a temperature of (-45°C)-(-40°C), heated to 38-42°C at a heating rate of 2-3°C / min, kept warm for 10-14 hours, then heated to 48-52°C at a heating rate of 0.2-0.4°C / min, kept warm for 1.5-2.5 hours, and then cooled to 18-22°C until the moisture content of the roselle flowers reaches 4-6%.

[0054] Drying the roselle flowers in a multi-stage heating and cooling manner can maximize the retention of the effective active ingredients in the roselle flowers, thereby enhancing the acne-removing, barrier-repairing, and oil secretion-reducing effects of the acne-removing and recurrence-preventing composition.

[0055] Specifically, the average particle size of the roselle flower is ≤150 μm; for example, it can be but not limited to 1 μm, 5 μm, 10 μm, 30 μm, 50 μm, 70 μm, 90 μm, 110 μm, 130 μm, 150 μm;

[0056] Specifically, the solid-liquid ratio of the roselle flower and the acidified ethanol solution is 1 g:30-40 mL; for example, it can be but not limited to 1 g:30 mL, 1 g:32 mL, 1 g:34 mL, 1 g:36 mL, 1 g:38 mL, 1 g:40 mL;

[0057] Specifically, the frequency of the ultrasonic extraction is 120-160 Hz; for example, it can be but not limited to 120 Hz, 125 Hz, 130 Hz, 135 Hz, 140 Hz, 145 Hz, 150 Hz, 155 Hz, 160 Hz;

[0058] Specifically, the ultrasonic extraction time is 25-35 min; for example, it can be but not limited to 25 min, 27 min, 29 min, 31 min, 33 min, 35 min;

[0059] Specifically, the temperature of the ultrasonic extraction is 45-55°C; for example, it can be but not limited to 45°C, 47°C, 49°C, 51°C, 53°C, 55°C;

[0060] Specifically, the pH of the acidified ethanol solution is 2.5-3.5; for example, it can be but not limited to 2.5, 2.7, 2.9, 3.1, 3.3, 3.5.

[0061] In some embodiments, the acidified ethanol solution is prepared by adding hydrochloric acid to an ethanol aqueous solution and mixing uniformly to obtain an acidified ethanol solution with a pH of 2.5-3.5.

[0062] Specifically, the amount of hydrochloric acid added is not particularly limited in the present disclosure, as long as the pH of the acidified ethanol solution is 2.5-3.5.

[0063] Specifically, the volume fraction of ethanol in the ethanol aqueous solution is 50-70%, for example, it can be 50%, 53%, 55%, 58%, 60%, 62%, 65%, 67%, or 70%.

[0064] In some embodiments, the preparation method of the Zizyphus joazeiro bark extract is as follows: adding Zizyphus joazeiro bark to an ethanol aqueous solution for extraction, performing solid-liquid separation after the extraction, and drying the obtained filtrate to obtain the Zizyphus joazeiro bark extract.

[0065] In the preparation method of the jujube bark extract, each parameter satisfies at least one of the following:

[0066] Specifically, the solid-liquid ratio of the jujube tree bark and the ethanol aqueous solution is 1g:10-20mL; for example, it can be but not limited to 1g:10mL, 1g:12mL, 1g:14mL, 1g:16mL, 1g:18mL, 1g:20mL;

[0067] Specifically, the volume percentage of ethanol in the ethanol aqueous solution is 50-70%; for example, it can be 50%, 53%, 55%, 58%, 60%, 62%, 65%, 67%, or 70%;

[0068] Specifically, the extraction method is one of leaching, reflux extraction and ultrasonic extraction.

[0069] Specifically, the temperatures for leaching, reflux extraction, and ultrasonic extraction are each independently 55-75°C, for example, 55°C, 57°C, 59°C, 61°C, 63°C, 65°C, 67°C, 69°C, 71°C, 73°C, or 75°C;

[0070] The extraction time is 40-56 hours, for example, but not limited to 40 hours, 44 hours, 48 hours, 52 hours, and 56 hours;

[0071] The reflux extraction time is 4-8 hours, for example, but not limited to 4 hours, 5 hours, 6 hours, 7 hours, and 8 hours;

[0072] The ultrasonic extraction time is 1-2 hours; the ultrasonic extraction power is 200-300W.

[0073] Specifically, the Hibiscus sabdariffa flower extract and / or Zizyphus joazeiro bark extract prepared by the above method can further improve the acne removal, repair and oil control effects of the acne removal and recurrence prevention composition.

[0074] In the preparation process of the roselle flower extract and the zizyphus joazeiro bark extract, the method of solid-liquid separation is not particularly limited, as long as the solid and liquid can be separated, such as filtration and centrifugation. In order to shorten the preparation time of the roselle flower extract and the zizyphus joazeiro bark extract, the present disclosure selects centrifugation as the method of solid-liquid separation.

[0075] Specifically, the centrifugal speed is 3000-5000 r / min, and the centrifugal time is 10-20 min.

[0076] Those skilled in the art can use means known in the art to uniformly mix epigallocatechin gallate glucoside, brazilin, Hibiscus sabdariffa flower extract, Ziziphus jojoba bark extract, linseed oil unsaponifiable matter and Guaiac wood extract to obtain an acne-removing and recurrence-preventing composition, such as mechanical stirring, homogenization, or ultrasound.

[0077] Specifically, the preparation method of the acne-removing and recurrence-preventing composition includes the following steps: thoroughly mixing epigallocatechin gallate glucoside, brazilian sappan wood, roselle flower extract, jujube bark extract, linseed oil unsaponifiable matter and guaiac extract to obtain the acne-recurring and recurrence-preventing composition.

[0078] After mixing, a uniform and stable acne-removing and recurrence-preventing composition is obtained, and the effective ingredients are fully mixed, which is conducive to the full release of the active ingredients.

[0079] In a second aspect, the invention provides the use of the acne-removing and recurrence-preventing composition in the preparation of cosmetics.

[0080] In a third aspect, a cosmetic is provided, comprising the acne-eliminating and recurrence-preventing composition, wherein the content of the acne-eliminating and recurrence-preventing composition in the cosmetic is 0.1-3 wt %.

[0081] The content of the acne-removing and recurrence-preventing composition in the cosmetic can be, but is not limited to, 0.1wt%, 0.3wt%, 0.5wt%, 0.7wt%, 0.9wt%, 1.2wt%, 1.4wt%, 1.6wt%, 1.8wt%, 2wt%, 2.3wt%, 2.5wt%, 2.8wt%, and 3wt%.

[0082] Adding a specific proportion of the acne-removing and recurrence-preventing composition to cosmetics can provide excellent acne-removing, skin-repairing, and oil-control effects. When the cosmetics of the present application contain less than 0.1 wt% of the acne-repairing and recurrence-preventing composition, sufficient acne-removing, skin-repairing, or oil-control effects cannot be expected. Furthermore, when the composition exceeds 3 wt%, undesirable reactions such as allergies may occur, or there may be concerns about skin safety.

[0083] In addition to using the acne-removing and recurrence-preventing composition as an active ingredient, the ingredients contained in the cosmetic composition of the present invention also include ingredients commonly used in cosmetic compositions, such as conventional adjuvants, such as antioxidants, stabilizers, solubilizers, vitamins, colorants, pigments, and fragrances and carriers.

[0084] The cosmetic composition of the present invention can be prepared as any formulation conventionally prepared in the art, and can be formulated into, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansers, oils, powdered foundations, emulsion foundations, waxy foundations, sprays, etc., but the present invention is not limited thereto. More specifically, the cosmetic composition of the present invention can be prepared into the following formulations: moisturizing lotions, nourishing lotions, nourishing creams, massage creams, essences, facial masks, eye creams, cleansing creams, cleansing foams, cleansing waters, moisturizing creams, fermented creams, ampoules, shampoos, conditioners, sprays, or powders.

[0085] When the preparation of the present invention is a paste, cream or gel, at least one selected from animal oils, vegetable oils, waxes, paraffin, starch, tragacanth gum, cellulose derivatives, polyethylene glycol, silicone, bentonite, silicon dioxide, talc and zinc oxide can be used as a carrier component.

[0086] When the preparation of the present invention is a powder or a spray, lactose, talc, silicon dioxide, aluminum hydroxide, calcium silicate or polyamide powder can be used as a carrier component. In particular, when the preparation of the present invention is a spray, a propellant (such as chlorofluorocarbons, propane / butane or dimethyl ether) can be additionally included.

[0087] When the preparation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier may be used as a carrier component. For example, at least one selected from the group consisting of water, ethanol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, butylene glycol, 1,3-butyl glycol oil, polyoxyethylene hydrogenated castor oil, glycerin, glycerin, fatty esters, phenoxyethanol, triethanolamine, polyethylene glycol, beeswax, polysorbate 60, sorbitan sesquioleate, paraffin, sorbitan stearate, glyceryl monostearate (lipophilic), stearic acid, glyceryl stearate / PEG-400 stearate, a carboxy polymer, sitosterol, polyglyceryl 2-oleate, ceramide, cholesterol, steareth-4, dicetyl phosphate, macadamia nut oil, carboxyvinyl polymer, xanthan gum, and a fatty acid ester of sorbitan can be used.

[0088] When the preparation of the present invention is a suspension, at least one selected from the following can be used as a carrier component: a liquid diluent such as water, ethanol, glycerol, butylene glycol or propylene glycol; a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitan esters and polyoxyethylene sorbitan esters, microcrystalline cellulose, hydroxyethylcellulose, sodium hyaluronate, phenoxyethanol, aluminum metahydroxide, bentonite, stearic acid, cetyl alcohol, glyceryl monostearate, polyoxyethylene sorbitan monostearate, sorbitan sesquioleate, glyceryl monostearate / glyceryl stearate / polyoxyethylene stearate, wax, paraffin, squalane, caprylic / capric triglyceride, carboxyvinyl polymer, triethanolamine, agar and tragacanth gum.

[0089] When the preparation of the present invention is a surfactant-containing cleanser, at least one selected from the group consisting of fatty alcohol sulfates, fatty alcohol ether sulfates, sulfosuccinic acid monoesters, isethionates, imidazolinium derivatives, methyl taurates, sarcosinates, fatty acid amide ether sulfates, alkylamido betaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives, and ethoxylated glyceryl fatty acid esters can be used as a carrier component.

[0090] In some embodiments, the cosmetic is an anti-acne lotion, and the anti-acne lotion comprises the following components in percentage by weight: 0.1-3% of an anti-acne recurrence prevention composition, 0.05-5% of a thickener, 3-20% of a moisturizer, 0.5-10% of an emulsifier, 0.2-5% of a preservative, 0.05-0.1% of a pH adjuster, and the remainder being water.

[0091] Specifically, the mass percentage of the acne-removing and recurrence-preventing composition can be, but is not limited to, 0.1%, 0.3%, 0.5%, 0.7%, 1.1%, 1.5%, 1.8%, 2%, 2.2%, 2.4%, 2.6%, 2.8%, and 3%;

[0092] Specifically, the mass percentage of the thickener may be, but is not limited to, 0.05%, 0.1%, 0.3%, 0.5%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, or 5%;

[0093] Specifically, the mass percentage of the moisturizing agent can be, but is not limited to, 3%, 5%, 7%, 9%, 11%, 13%, 15%, 17%, or 20%;

[0094] Specifically, the mass percentage of the emulsifier can be, but is not limited to, 0.5%, 1%, 1.5%, 3%, 4%, 5%, 7%, 8%, or 10%;

[0095] Specifically, the mass percentage of the preservative can be, but is not limited to, 0.2%, 0.5%, 1%, 1.5%, 2%, 3%, 4%, or 5%;

[0096] Specifically, the mass percentage of the pH adjuster can be, but is not limited to, 0.05%, 0.08%, or 0.1%.

[0097] In some embodiments, the thickener comprises at least one of xanthan gum, carbomer, ammonium acrylamide dimethyl taurate / VP copolymer, and cellulose;

[0098] In some embodiments, the moisturizing agent includes at least one of glycerin, butylene glycol, and sodium hyaluronate;

[0099] In some embodiments, the emulsifier includes at least one of coconut oil alcohol-caprylate / caprate, polydimethylsiloxane, hydrogenated palm kernel oil, cetearyl alcohol, polyglyceryl-6 distearate, and polymethylsilsesquioxane;

[0100] In some embodiments, the preservative includes at least one of 1,3-propylene glycol, 1,2-hexanediol, and p-hydroxyacetophenone;

[0101] In some embodiments, the pH adjuster comprises at least one of arginine and disodium edetate.

[0102] The cosmetics of the present invention can be prepared by any suitable method known in the art. For example, the cosmetics can be prepared using equipment such as dissolving tanks, emulsifying pots, dispersers, and delivery pumps commonly used in the cosmetics field, according to processes known in the art. For example, a water-soluble substance can be first put into an aqueous phase dissolving pot, and an oil-soluble substance (such as sunscreen, grease) can be put into an oil phase dissolving pot, and the temperature of the two pots can be heated to about 80°C. For raw materials that are easy to agglomerate, they can be pre-dispersed with a disperser; such as powder-containing substances (such as zinc oxide, titanium dioxide), they can be dispersed with a suitable solvent and dispersant and homogenized for 5-10 minutes, and the oil phase can be added and homogenized again for 5-10 minutes; after homogenization is completed, the oil phase and the water phase are delivered to the emulsifying pot for homogenization and emulsification for about 5-15 minutes; after emulsification is completed, the temperature of the material body is reduced to room temperature, and optionally, flavors, preservatives, etc. are added, and the pH of the product is adjusted as needed; after the relevant test indicators are qualified, the material can be filled and discharged.

[0103] The above preparation process is merely exemplary, and those skilled in the art may add, subtract or adjust it according to the dosage form requirements to prepare various dosage forms such as spray, emulsion, gel / jelly, cream, cushion / foundation, etc.

[0104] In order to further understand the present application, the antioxidant composition of the present application, its preparation method and its effects are further described in detail below with reference to specific examples. All raw materials involved in the present application can be obtained through commercial purchase.

[0105] The raw materials used in the examples and comparative examples are described below, but are not limited to these materials:

[0106] The roselle pollen is prepared by pre-freezing the roselle flowers for 3 hours and then placing them in a vacuum freeze drying oven with a vacuum degree of 80 Pa and a cold trap temperature of -40°C. The heating plate is heated to 40°C over 30 minutes, kept warm for 12 hours, then heated to 50°C over 30 minutes, kept warm for 2 hours, and then cooled to 20°C over 30 minutes. The drying process is continued until the moisture content of the roselle flowers reaches 5%. The flowers are then crushed and passed through a 100-mesh sieve. The sieved material is the roselle pollen, which is placed in a desiccator for later use.

[0107] The acidified ethanol solution 1 is homemade, and its preparation method is as follows: hydrochloric acid is added to a 60% volume fraction ethanol aqueous solution, and mixed uniformly to obtain an acidified ethanol solution 1 with a pH of 3;

[0108] The acidified ethanol solution 2 is homemade, and its preparation method is as follows: hydrochloric acid is added to a 50% volume fraction ethanol aqueous solution, and mixed uniformly to obtain an acidified ethanol solution 2 with a pH of 3;

[0109] The acidified ethanol solution 3 is homemade, and its preparation method is as follows: hydrochloric acid is added to a 70% volume fraction ethanol aqueous solution, and mixed uniformly to obtain an acidified ethanol solution 3 with a pH of 3;

[0110] Roselle flower extract 1 is homemade, and its preparation method is as follows: Roselle pollen is added to acidified ethanol solution 1, with the solid-liquid ratio of Roselle pollen to acidified ethanol solution 1 being 1 g:35 mL, and extraction is performed under 140 kHz and 50° C. ultrasonication for 30 min, and the extraction is repeated 3 times. After the extraction is completed, the obtained product is centrifuged at 3000 r / min for 15 min, and the supernatant is concentrated by rotary rotation to remove ethanol, thereby obtaining Roselle flower extract 1;

[0111] Roselle flower extract 2 is homemade, and its preparation method is as follows: Roselle pollen is added to acidified ethanol solution 2, with the solid-liquid ratio of Roselle pollen to acidified ethanol solution 2 being 1 g:35 mL, and extraction is performed under 120 kHz, 50°C ultrasonic conditions for 30 min, and the extraction is repeated 3 times. After the extraction is completed, the obtained product is centrifuged at 3000 r / min for 15 min, and the supernatant is collected and concentrated by rotary evaporation to remove ethanol, thereby obtaining Roselle flower extract 2;

[0112] Roselle flower extract 3 is homemade, and its preparation method is as follows: Roselle pollen is added to acidified ethanol solution 3, with the solid-liquid ratio of Roselle pollen to acidified ethanol solution 3 being 1 g:35 mL, and extraction is performed under ultrasonic conditions at 160 kHz and 50°C for 30 minutes, and the extraction is repeated three times. After the extraction, the obtained product is centrifuged at 3000 r / min for 15 minutes, and the supernatant is concentrated by rotary evaporation to remove ethanol, thereby obtaining Roselle flower extract 3;

[0113] Ziziphus joazeiro bark extract 1 is homemade, and its preparation method is as follows:

[0114] Commercially available jujube bark was dried to constant weight, crushed, and passed through a 100-mesh sieve. The sieve residue was collected, and 1 g of the sieve residue powder was added to 15 mL of 70% ethanol solution and immersed at 60°C for 48 h. After the immersion, the mixture was filtered, and the filtrate was collected and concentrated under reduced pressure to dryness to obtain jujube bark extract 1.

[0115] Ziziphus joazeiro bark extract 2 is homemade, and its preparation method is as follows:

[0116] Commercially available Zizyphus joazeiro bark was dried to constant weight, crushed, and passed through a 100-mesh sieve. The sieve residue was collected, and 1 g of the sieve residue powder was added to 15 mL of 70% ethanol solution. The mixture was refluxed at 60°C for 6 h. After the extraction, the mixture was filtered, and the filtrate was collected and concentrated under reduced pressure to dryness to obtain Zizyphus joazeiro bark extract 2.

[0117] Ziziphus joazeiro bark extract 3 is homemade, and its preparation method is as follows:

[0118] Commercially available Zizyphus joazeiro bark was dried to constant weight, crushed, and passed through a 100-mesh sieve. The undersize fraction was collected. 1 g of the undersize fraction powder was weighed and added to 15 mL of 70% ethanol solution. Ultrasonic extraction was performed at 60°C and 266 W of ultrasonic power for 60 min. After the ultrasonic extraction, the extract was filtered, and the filtrate was collected and concentrated under reduced pressure to dryness to obtain Zizyphus joazeiro bark extract 3.

[0119] Epigallocatechin Gallate Glucoside: Trade Name: EGCG, manufacturer: Givaudan;

[0120] Brazilian hematoxylin: derived from hematoxylin, 98%, manufacturer: Bailingwei;

[0121] Linseed oil unsaponifiables: Trade name: Manufacturer: Aoyuan;

[0122] Guaiac wood extract: brand L2-DZ-SCB3675-X, manufacturer: Zhenghe.

[0123] Examples 1-6 and Comparative Examples 1-6

[0124] The present invention provides an acne-removing and recurrence-preventing composition suitable for acne-sensitive skin. The components of the acne-removing and recurrence-preventing composition are shown in Table 1.

[0125] The preparation method of the acne-removing and recurrence-preventing composition of Examples 1-6 and Comparative Examples 1-6 is as follows: uniformly mix the components according to the weight parts in Table 1 to obtain the acne-recurrence-preventing composition.

[0126] Table 1

[0127]

[0128]

[0129] Example 7

[0130] The embodiment of the present invention provides an acne-removing and recurrence-preventing composition suitable for acne-sensitive skin. The acne-removing and recurrence-preventing composition of this embodiment differs from that of Example 1 only in that the roselle flower extract 1 is replaced by the roselle flower extract 2.

[0131] Example 8

[0132] The embodiment of the present invention provides an acne-removing and recurrence-preventing composition suitable for acne-sensitive skin. The acne-removing and recurrence-preventing composition of this embodiment differs from that of Example 1 only in that the roselle flower extract 1 is replaced by the roselle flower extract 3.

[0133] Example 9

[0134] The embodiment of the present invention provides an acne-removing and recurrence-preventing composition suitable for acne-sensitive skin. The acne-removing and recurrence-preventing composition of this embodiment differs from that of Example 1 only in that the zizyphus joazeiro bark extract 2 is used instead of the zizyphus joazeiro bark extract 1.

[0135] Example 10

[0136] The embodiment of the present invention provides an acne-removing and recurrence-preventing composition suitable for acne-sensitive skin. The acne-removing and recurrence-preventing composition of this embodiment differs from that of Example 1 only in that Zizyphus joazeiro bark extract 3 is used instead of Zizyphus joazeiro bark extract 1.

[0137] Comparative Example 7

[0138] The embodiment of the present invention provides an acne-removing and recurrence-preventing composition suitable for acne-sensitive skin. The acne-recurrence-preventing composition described in this comparative example differs from that in Example 1 only in that epigallocatechin gallate is used instead of epigallocatechin gallate glucoside.

[0139] Comparative Example 8

[0140] The embodiment of the present invention provides an acne-removing and recurrence-preventing composition suitable for acne-sensitive skin. The acne-recurrence-preventing composition described in this comparative example differs from that in Example 1 only in that acacetin is used instead of brazilin.

[0141] Comparative Example 9

[0142] The embodiment of the present invention provides an acne-removing and recurrence-preventing composition suitable for acne-sensitive skin. The acne-recurring and recurrence-preventing composition described in this comparative example differs from that in Example 1 only in that hibiscus extract is used instead of roselle flower extract 1.

[0143] Comparative Example 10

[0144] The embodiment of the present invention provides an acne-removing and recurrence-preventing composition suitable for acne-sensitive skin. The acne-recurring and recurrence-preventing composition described in this comparative example differs from that in Example 1 only in that magnolia bark extract is used instead of zizyphus joazeiro bark extract 1.

[0145] Comparative Example 11

[0146] The embodiment of the present invention provides an acne-removing and recurrence-preventing composition suitable for acne-sensitive skin. The acne-removing and recurrence-preventing composition described in this comparative example differs from that in Example 1 only in that unsaponifiable matter of linseed oil is replaced by unsaponifiable matter of olive oil.

[0147] Comparative Example 12

[0148] The embodiment of the present invention provides an acne-removing and recurrence-preventing composition suitable for acne-sensitive skin. The acne-recurring and recurrence-preventing composition described in this comparative example differs from that in Example 1 only in that Tetrana chinensis extract is used instead of Guaiacula fragrans extract.

[0149] Application Examples 1-11, Comparative Application Examples 1-12, and Blank Application Examples

[0150] The application examples, comparative application examples, and blank application examples of the present invention provide an acne-removing emulsion, the components (mass percentages) of the acne-removing emulsion are shown in Table 2; wherein, the acne-relapse-preventing compositions used in Application Examples 1-10 are respectively the acne-relapse-preventing compositions prepared in Examples 1-10, and the acne-relapse-preventing compositions used in Comparative Application Examples 1-12 are respectively the acne-relapse-preventing compositions prepared in Comparative Examples 1-12; the acne-relapse-preventing composition used in Application Example 11 is the acne-relapse-preventing composition prepared in Example 1;

[0151] The preparation methods of the anti-acne lotions of the application examples, comparative application examples, and blank application examples are as follows:

[0152] (1) Component A is mixed with water, heated to 80°C, and homogenized at 1200 rpm for 5 minutes. After homogenization, the mixture is kept warm for later use to obtain prefabricated component A;

[0153] (2) Mix component B, heat to 80°C, and homogenize at 1200 rpm for 5 minutes. After homogenization, keep warm and set aside to obtain prefabricated component B;

[0154] (3) Mix the C components and heat to 60°C to melt to obtain a prefabricated C component;

[0155] (4) The preformed component A is heated to 80°C, and the preformed component B is added at a speed of 300 rpm, and stirred and mixed. Then, the temperature is cooled to 60°C, and the preformed component C is added at a speed of 300 rpm and stirred and mixed. Then, the temperature is cooled to below 45°C, and the acne-recurrence prevention composition and essence are added and stirring is continued for 5 minutes. Finally, arginine is added to adjust the pH to 5.0-6.5. Stirring can be stopped, and the material is discharged to obtain an acne-removing lotion.

[0156] Table 2

[0157]

[0158]

[0159] Effect Example 1: Test of inflammatory factor IL-1β level

[0160] This effect example explores the anti-inflammatory effects of the acne-recurrence prevention compositions prepared in Examples 1-10 and Comparative Examples 1-12.

[0161] Experimental Materials: The cell line used was mouse macrophage RAW264.7, purchased from the Cell Resource Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences. Fetal bovine serum (FBS, Gibco), Dulbecco's modified Eagle's medium (DMEM, Gibco), dimethyl sulfoxide (DMSO, Sigma), phosphate-buffered saline (PBS, Gibco), thiazolyl blue (MTT, Sigma), and a mouse tumor necrosis factor-α enzyme-linked immunosorbent assay kit (Mouse TNF-α ELISA kit, Boster) and a mouse interleukin-1β ELISA kit (Mouse IL-1β ELISA kit, Boster) were used. The experimental group settings are shown in Table 3. The specific procedures are as follows:

[0162] (1) Inoculation: Cells were seeded into 24-well plates and incubated at 37°C in a 5% CO2 incubator for 18-24 h;

[0163] (2) Solution preparation: Prepare the test substance and positive control according to Table 3;

[0164] (3) Sampling: After the cells have been plated and grown for 18-24 hours in a 24-well plate according to the experimental grouping and concentration settings in Table 3, sample the cells in the plates. Each treatment group has three replicate wells. Cell culture medium is added to both the blank and negative control groups. Cell culture medium containing the corresponding concentration of sample is added to the sample groups. The cells are then cultured in a 37°C, 5% CO2 incubator for an additional 18-24 hours.

[0165] (4) LPS induction: After 18-24 h of culture, the culture medium in the plate was aspirated and the cells were gently washed once with PBS. Cell culture medium was added to the blank control group, and cell culture medium containing LPS was added to the negative control group, sample group, and positive control group. The cells were cultured in a 37°C, 5% CO2 incubator for 18-24 h, and the supernatant was collected.

[0166] (5) Detection of inflammatory factor content: Take the cell supernatant of each well and detect the cell inflammatory factor content according to the ELISA kit operating instructions;

[0167] (6) Data processing: All data obtained in the experiment were processed and plotted using Excel software. Statistical analysis was performed using SPSS 17.0. One-way analysis of variance (ANOVA) was used for comparison between groups. Significant differences were considered when P < 0.05. The results are shown in Table 4.

[0168] Table 3

[0169]

[0170] Table 4

[0171]

[0172]

[0173] Note: Compared with the blank control group, the significance is indicated by #, and P value < 0.0001 is indicated by ####. Compared with the negative control group, the significance is indicated by *, and P value < 0.05 is indicated by *, P value < 0.01 is indicated by **, P value < 0.0001 is indicated by ****, and P value > 0.05 is indicated by ns.

[0174] Interleukin-1β (IL-1β) plays an important role in the immune regulation and inflammatory response process and is considered to be one of the main endogenous mediators of inflammatory response and fever reaction during the process. IL-1β is a key inflammatory mechanism that drives the host's response to Pseudomonas acnes infection, aggravating skin acne. This experiment is based on the LPS-induced mouse macrophage (RAW264.7) inflammation model to evaluate the ability of Examples 1-10 and Comparative Examples 1-12 to reduce the expression and secretion of cellular inflammatory factors, and then predict their ability to inhibit further occurrence of inflammatory response, in order to prove the effect of the acne-removing and recurrence-preventing composition in removing acne.

[0175] From the test results in Table 4, it can be seen that after applying the acne-removing and recurrence-preventing compositions of Examples 1-10 provided by the present invention, the average concentration of cellular inflammatory factors is 31.47-38.71 pg / mL, indicating that the acne-recurrence-preventing compositions of the present invention have an inhibitory effect on the overexpression of IL-1β induced by lipopolysaccharide (LPS), and the inhibitory effects are significant, among which Example 1 has the best inhibitory effect.

[0176] Comparing Example 1 and Comparative Examples 1-12, it can be seen that the lack of any one of epigallocatechin gallate glucoside, brazilin, roselle flower extract, jujube bark extract, linseed oil unsaponifiable matter, and guaiac extract or the use of other functional analogs for replacement, the average concentration of cellular inflammatory factors is greater than 40 pg / mL, indicating that the lack of any one of epigallocatechin gallate glucoside, brazilin, roselle flower extract, jujube bark extract, linseed oil unsaponifiable matter, and guaiac extract or the use of other functional analogs for replacement will lead to a decrease in the anti-inflammatory effect of the acne anti-recurrence composition.

[0177] Effect Example 2: Inhibition Test of Propionibacterium acnes

[0178] This effect example explores the inhibitory effect of the acne-recurrence prevention composition prepared in Examples 1-10 and Comparative Examples 1-12 on Propionibacterium acnes.

[0179] The 96-well plate crystal violet staining method was used. The sample was added to a certain concentration of bacterial solution so that the final dosage concentration was 200 μg / mL. The sample was cultured under anaerobic conditions at 37°C for 6 days, the upper planktonic cells were discarded, washed with sterile water, and then fixed with formaldehyde for 10 minutes. After removal, the cells were air-dried for 5 minutes. The biofilm was stained with 0.1% crystal violet for 20 minutes. After removing the dye, 955 ethanol was added for decolorization for 15 minutes. 100 μL of the decolorized solution was placed in a blank well plate, and the absorbance was measured at a wavelength of 570 nm using a microplate reader. The biofilm of the control group without the addition of the composition was taken as 100%, and the percentage of biofilm formation in each experimental group was calculated. The results are shown in Table 5.

[0180] Table 5

[0181]

[0182]

[0183] Note: Compared with the blank control group, significance is indicated by #, P value < 0.05 is indicated by *, P value < 0.01 is indicated by **, P value < 0.0001 is indicated by ****, and P value > 0.05 is indicated by ns.

[0184] The total detection rate of Propionibacterium acnes in facial acne lesions is higher than that in normal facial skin. Propionibacterium acnes is the primary bacterial species isolated from facial lesions in acne. Inhibiting the activity of P. acnes can effectively improve acne severity. This experiment tested the inhibition rate of P. acnes biofilm to evaluate the antibacterial ability of the acne-removing and recurrence-preventing composition.

[0185] From the experimental results in Table 5, it can be seen that the biofilm formation rate of the acne-recurrence prevention composition of Examples 1-10 of the present invention is 50.13-63.57%, indicating that the acne-recurrence prevention composition of the present invention has a significant inhibitory effect on Propionibacterium acnes.

[0186] Comparing Example 1 and Comparative Examples 1-12, it can be seen that the biofilm formation rate is greater than 88% when any one of epigallocatechin gallate glucoside, brazilin, roselle flower extract, jujube bark extract, linseed oil unsaponifiable matter, and guaiac extract is lacking or replaced with other functional analogs, indicating that the lack of any one of epigallocatechin gallate glucoside, brazilin, roselle flower extract, jujube bark extract, linseed oil unsaponifiable matter, and guaiac extract or the use of other functional analogs for replacement will result in a significant decrease in the inhibitory effect of the acne-removing and recurrence-preventing composition on Propionibacterium acnes.

[0187] Effect Example 3 Human Efficacy Test

[0188] This effect example tests the improvement effects of Application Examples 1-11, Comparative Application Examples 1-12 and a blank application example on human acne, skin moisture content, redness and oil.

[0189] The specific test methods are as follows:

[0190] According to the "Technical Specifications for Safety of Cosmetics" (2015), 184 volunteers aged 45-60 with sensitive skin (lactic acid stinging score > 3) were selected and randomly divided into 23 groups, with 8 people in each group. The volunteers had to have at least 2-3 obvious acne. Under normal circumstances, the volunteers continued to use the product on their entire face for 7 days (D7), and then stopped using it for 7 days (T7); every morning and evening after cleansing and moisturizing, the volunteers took an appropriate amount of lotion and applied it to the face, massaging it for absorption. The acne area could be overlapped and applied to the entire face once in the morning and evening, and follow-up visits were conducted every day. On the day of the volunteer's visit, the test area washed its face with clean water without applying any product. It was placed in an air-conditioned room with a temperature of 21±1°C and a humidity of 50±10% for 20 minutes. Improvement in facial acne indicators was quantified and photographed using instruments including the Visia 7 (IPP) test for facial redness a*, acne counts (categorized as comedones and papules, using the VISIA and physician assessment, respectively), the Tewameter TMHex test for transepidermal water loss (TEWL), and the Sebumeter SM 815 test for oil content. The improvement rate before and after use = (X pre-use mean - X post-use mean) / X pre-use mean × 100%; the calculated results are shown in Table 6.

[0191] Table 6

[0192]

[0193]

[0194] Note: * indicates that the difference is statistically significant compared with the blank application example (P < 0.05).

[0195] From the experimental results in Table 6, it can be seen that when the acne-removing emulsions of Examples 1-11 of the present invention are used, the D7 improvement rate of facial acne in acne patients with sensitive skin is 38.1-43.8%, and the T7 improvement rate is 17.2-23.5%; the D7 improvement rate of papules is 28.5-34.6%, and the T7 improvement rate is 15.1-19.8%; the D7 improvement rate of TEWL is 15.3-19.4%, and the T7 improvement rate is 7.6-11.3%; the D7 improvement rate of facial red area a* is 24.0-30.8%, and the T7 improvement rate is 15.2-19.5%; the D7 improvement rate of oil content is 22.0-25.8%, and the T7 improvement rate is 14.3-18.3%; This shows that the application of the acne-removing and recurrence-preventing composition of the present invention can effectively improve acne, papules, a*, TEWL and oil content of sensitive skin; that is, the acne-removing and recurrence-preventing composition of the present invention has good acne-removing, repairing and oil-control effects.

[0196] From Application Example 1 and Comparative Application Examples 1-12, it can be seen that when any one of epigallocatechin gallate glucoside, brazilin, roselle flower extract, jujube bark extract, linseed oil unsaponifiable matter, and guaiac extract is missing or replaced with other functional analogs, the D7 improvement rate of acne is less than 19%, and the T7 improvement rate is less than 4%; the D7 improvement rate of papules is less than 13%, and the T7 improvement rate is less than 5%; the D7 improvement rate of TEWL is less than 6.5%, and the T7 improvement rate is less than 1%; the D7 improvement rate of facial red area a* is less than 10%. The improvement rate of D7 in terms of oil content is less than 12%, and the improvement rate of T7 is less than 3%; the improvement rate of D7 in terms of oil content is less than 10%, and the improvement rate of T7 is less than 3%; this indicates that the lack of any one of epigallocatechin gallate glucoside, brazilian sappan wood, roselle flower extract, jujube bark extract, linseed oil unsaponifiable matter, and guaiac extract, or the use of other functional analogues for replacement, will result in the acne-removing and recurrence-preventing composition having no significant improvement on skin acne, papules, a*, TEWL, and oil content, that is, the acne-removing, repairing, and oil-control effects of the acne-removing and recurrence-preventing composition are significantly reduced.

[0197] Finally, it should be noted that the above embodiments are used to illustrate the technical solutions of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A composition for removing acne and preventing recurrence, characterized in that: The acne-recurrence prevention composition is composed of the following components in parts by weight: 0.01-3 parts of epigallocatechin gallate glucoside, 0.001-0.3 parts of brazilian sappan wood, 1-15 parts of Hibiscus sabdariffa flower extract, 0.1-1 parts of Zizyphus joazeiro bark extract, 0.01-3 parts of linseed oil unsaponifiable matter, and 1-15 parts of Guaiac wood extract; The preparation method of the roselle flower extract comprises: adding roselle pollen to an acidified ethanol solution, wherein the solid-liquid ratio of roselle pollen to acidified ethanol solution is 1 g:35 mL; extracting under ultrasonic conditions at 140 kHz and 50° C. for 30 minutes, repeating the extraction three times; after the extraction, centrifuging the obtained product at a speed of 3000 r / min for 15 minutes, and concentrating the supernatant to remove ethanol, thereby obtaining the roselle flower extract; wherein the preparation method of the acidified ethanol solution comprises: adding hydrochloric acid to an ethanol aqueous solution having a volume fraction of 60%, mixing uniformly, and obtaining an acidified ethanol solution having a pH of 3; The preparation method of the jujube bark extract comprises the following steps: taking commercially available jujube bark, drying it to a constant weight, crushing it, passing it through a 100-mesh sieve, collecting the sieve residue, weighing 1 g of the sieve residue powder, adding it to 15 mL of a 70% ethanol solution, and immersing it at 60° C. for 48 hours. After the immersion is completed, filtering is performed, collecting the filtrate, and concentrating and drying it under reduced pressure to obtain the jujube bark extract.

2. The acne-removing and recurrence-preventing composition according to claim 1, wherein: The acne-recurrence prevention composition is composed of the following components in parts by weight: 0.05-1 part of epigallocatechin gallate glucoside, 0.005-0.1 part of brazilian sappan wood, 3-10 parts of Hibiscus sabdariffa flower extract, 0.1-0.5 part of zizyphus joazeiro bark extract, 0.05-1 part of linseed oil unsaponifiable matter, and 1-8 parts of guaiac wood extract.

3. The acne-removing and recurrence-preventing composition according to claim 1, wherein: The acne-recurrence prevention composition is composed of the following components in parts by weight: 0.5-1 parts of epigallocatechin gallate glucoside, 0.01-0.1 parts of brazilian sappan wood, 3-8 parts of Hibiscus sabdariffa flower extract, 0.15-0.5 parts of zygophyllum bark extract, 0.08-0.5 parts of linseed oil unsaponifiable matter, and 2-5 parts of guaiac wood extract.

4. Use of the acne-removing and recurrence-preventing composition according to any one of claims 1 to 3 in the preparation of cosmetics.

5. A cosmetic, characterized in that: The cosmetic comprises the acne-eliminating and recurrence-preventing composition according to any one of claims 1 to 3, and the content of the acne-eliminating and recurrence-preventing composition in the cosmetic is 0.1-3 wt%.

6. The cosmetic according to claim 5, wherein The cosmetic is an anti-acne lotion, and the anti-acne lotion comprises the following components in percentage by weight: 0.1-3% of an anti-acne recurrence prevention composition, 0.05-5% of a thickener, 3-20% of a moisturizer, 0.5-10% of an emulsifier, 0.2-5% of a preservative, 0.05-0.1% of a pH regulator, and the balance being water.

7. The cosmetic according to claim 6, wherein The thickener comprises at least one of xanthan gum, carbomer, ammonium acrylamide dimethyl taurate / VP copolymer, and cellulose; And / or, the moisturizing agent includes at least one of glycerin, butylene glycol, and sodium hyaluronate; And / or, the emulsifier includes at least one of coconut oil alcohol-caprylate / caprate, polydimethylsiloxane, hydrogenated palm kernel oil, cetearyl alcohol, polyglyceryl-6 distearate, and polymethylsilsesquioxane; and / or, the preservative comprises at least one of 1,3-propylene glycol, 1,2-hexanediol, and p-hydroxyacetophenone; And / or, the pH regulator includes at least one of arginine and disodium edetate.

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