Oil-controlling and anti-dandruff composition, use and hair product

Abstract

The present invention belongs to the technical field of washing and protecting products, and discloses an oil-controlling and anti-dandruff composition, use and hair products. The oil-controlling and anti-dandruff composition comprises the following components by weight percentage: 6-10% of channa ulmoides extract, 2-6% of emblica extract, 1-5% of safflower extract, 0.1-3% of lotus seed extract, and 0.1-3% of sophora flavescens root extract. The present invention discloses an oil-controlling and anti-dandruff composition, which produces a synergistic effect of improving the anti-dandruff and oil-control effects by compounding channa ulmoides extract, emblica extract, safflower extract, lotus seed extract and sophora flavescens root extract, and has excellent anti-dandruff and oil-control effects.

Description

Technical Field

[0001] The invention relates to the technical field of washing and care products, and in particular to an oil-controlling and anti-dandruff composition, uses thereof and a hair product. Background Art

[0002] Based on the current eating habits of most people who like to eat greasy, spicy, high-sugar, and high-fat foods, as well as irregular lifestyles, excessive mental work, etc., it is easy to stimulate the sebaceous glands on the head to secrete more oil and dandruff, thus affecting the health of the head hair.

[0003] Therefore, it is necessary to develop dandruff removal and oil control products to meet consumers' hair care needs; dandruff removal requires penetrating deep into the scalp to thoroughly clean oil, dirt and dandruff, while moisturizing the scalp to reduce the itching and discomfort caused by dandruff; while oil control refers to regulating the oil secretion of the scalp to avoid greasy hair and extend the interval between shampooing.

[0004] For example, prior art 1: Chinese patent application 202210311189.0 discloses an oil-control anti-dandruff shampoo, which is composed of water and phases A to G, adopts a mild cleansing formula, and the components work together to gently clean the scalp and hair, reduce itching and hair loss, add salicylic acid, effectively reduce dandruff, and promote the efficient absorption of active ingredients, thereby achieving hair nourishment, hair care, and hair strengthening, and is suitable for long-term use.

[0005] Prior art 1 can achieve a good anti-dandruff effect through a multi-phase compound composition, but prior art 1 does not record its oil control mechanism and effect, because its oil control effect is unclear, and the salicylic acid added in prior art 1 is highly irritating to the skin and the dosage needs to be strictly controlled.

[0006] At present, natural plants are a better choice for mild and low-irritation cleaning products. For example, prior art 2: Chinese patent application 202011354603.3 discloses an oil-control and anti-dandruff composition and its application, wherein the oil-control and anti-dandruff composition includes zinc pyrithione, Melaleuca alternifolia leaf oil, saccharide isomers, capryloyl glycine and Eucalyptus globulus leaf oil. The components synergize to make the oil-control and anti-dandruff composition effectively inhibit fungi and oil secretion, achieve efficient dandruff removal, and have moisturizing and anti-itching effects, low irritation and high safety.

[0007] Prior art 2 uses plant extracts such as Melaleuca alternifolia leaf oil and Eucalyptus globulus leaf oil to compound other components to form an oil-controlling and anti-dandruff composition, which has good anti-dandruff and oil-control effects. However, prior art 2 still compounds highly irritating components such as zinc pyrithione to achieve the functions of anti-dandruff and oil-control.

[0008] Therefore, it is necessary to find a composition formed by compounding pure plant extracts, which can give full play to the anti-dandruff and oil-control effects of the composition and has the advantages of low irritation. Summary of the invention

[0009] One of the purposes of the present invention is to provide an oil-controlling and anti-dandruff composition, which is compounded with a variety of plant extracts to exert a synergistic anti-dandruff and oil-control effect. The multiple components cooperate synergistically from the mechanisms of cleaning oil and dandruff to regulating scalp oil secretion to effectively improve the anti-dandruff and oil-control effects of the composition.

[0010] Another object of the present invention is to provide a use of an oil-controlling and anti-dandruff composition, which can be used in the preparation of washing and care products to exert its excellent anti-dandruff and oil-control effects and has the advantage of low irritation, and has a good market application prospect.

[0011] At the same time, the present invention also provides a shampoo containing the oil-controlling and anti-dandruff composition, which has extremely strong anti-dandruff and oil-control effects.

[0012] To achieve the above object, the present invention provides an oil-controlling and anti-dandruff composition, which comprises the following components by weight percentage:

[0013] 6-10% of Channa intestine extract, 2-6% of Phyllanthus emblica extract, 1-5% of Safflower extract, and 0.1-3% of Lotus seed extract.

[0014] The extract of Channa intestine mainly contains flavonoids, which have strong antioxidant and anti-inflammatory effects, can regulate sebum secretion to relieve the phenomenon of excessive oil, and at the same time, can inhibit the oxidation of oil and help maintain the refreshing state of the skin;

[0015] Emblica extract is rich in tannic acid and other polyphenolic compounds, which have astringent effects and can help reduce sebum secretion. It can also fight inflammation and improve scalp texture. It is also rich in antioxidant ingredients such as vitamin C, which can help improve skin condition and resist free radical damage.

[0016] Safflower extract contains safflower glycosides, which can dilate blood vessels, improve microcirculation, and regulate the function of sebaceous glands. It can help control the production of excess oil, thereby achieving the effect of oil control. It is also rich in polyphenol compounds, which can fight skin inflammation and help inhibit the growth of fungi or other bacteria such as Malassezia that cause dandruff, thereby improving scalp conditions.

[0017] Lotus seed is also known as lotus seed. Lotus seed extract is rich in various alkaloids, among which liensinine has a good inhibitory effect on inflammatory factors, thereby soothing the scalp, reducing inflammation caused by excessive oil, and playing an antibacterial and anti-inflammatory role. At the same time, the natural ingredients in lotus seeds help maintain the moisture balance of the skin and prevent dryness caused by oil control.

[0018] The present invention compounds Channa intestine extract, Emblica officinalis extract, Safflower extract and Lotus seed extract to control sebum, oil secretion and oxidation, inhibit fungal and bacterial growth, and cooperate with anti-inflammation and balance skin conditions. Each mechanism cooperates with each other, thereby synergistically improving the oil control and anti-dandruff effects of the oil control and anti-dandruff composition of the present invention.

[0019] Preferably, the oil-controlling and anti-dandruff composition of the present invention further comprises 0.1-3% of Sophora flavescens root extract.

[0020] Sophora flavescens root extract is extracted from the root of Sophora flavescens. It is rich in matrine and has the effect of clearing away heat and drying dampness. It has a good anti-inflammatory effect on common skin problems such as skin redness, inflammation, etc. It has a good relieving effect on skin itching. At the same time, it has a significant inhibitory effect on bacterial growth, balances oil secretion, unclogs and tightens pores, removes toxins and impurities in the skin, promotes the growth and repair of damaged vascular nerve cells, and restores the vitality of subcutaneous capillary cells.

[0021] Furthermore, the oil-controlling and anti-dandruff composition also contains a solvent, and may also contain appropriate amounts of preservatives, pH regulators and other substances.

[0022] Furthermore, the Channa intestine extract, Phyllanthus emblica extract, Safflower extract, and Lotus seed extract are obtained by composite extraction through the following steps:

[0023] Step 1: Grind the chrysanthemum intestine, emblica fruit, safflower and lotus seeds into powder;

[0024] Step 2: extracting the powder with water, concentrating it, and precipitating it with alcohol to obtain an alcohol precipitate;

[0025] Step 3: centrifuging the alcohol precipitate to obtain a centrifuge, and then concentrating the centrifuge to obtain an ethanol-free concentrate;

[0026] Step 4: Add polyol and water to the ethanol-free concentrated solution, stir and mix to obtain a mixed solution, and then perform ultrafiltration to obtain an ultrafiltration effluent.

[0027] Preferably, the mass percentage of the ethanol-free concentrated liquid in the mixed liquid is 15-20wt%, and the mass percentage of the polyol is 20-50wt%.

[0028] More preferably, a proper amount of preservative may be added to the mixed solution.

[0029] More preferably, the polyol is at least one of glycerol, butylene glycol and propylene glycol.

[0030] Preferably, the specific operation of the ultrafiltration is: transferring the mixed liquid to the ultrafiltration equipment, the ultrafiltration membrane pore size is 0.1um, the ultrafiltration pressure is 0.24-0.30MPa, starting the ultrafiltration and turning on the cooling circulating water, controlling the ultrafiltration temperature at 38-45°C, and collecting the ultrafiltrate.

[0031] Furthermore, the specific operation of step 2 is: adding 8-10 times the weight of the powder of water to the powder, extracting with circulating water at an extraction temperature of 63-68°C for 3 hours to obtain an extract, and then concentrating the extract through a single-effect concentrator at a concentration temperature of 65-68°C to 15-30wt% of the extract mass to obtain a concentrated solution, adding 95% ethanol with the same mass as the concentrated solution to the concentrated solution, stirring for 1 hour and then standing for 1 hour to obtain an alcohol precipitate.

[0032] Furthermore, the specific operation of step 3 is: centrifuging the alcohol precipitate to obtain a centrifuge, and then concentrating it at a concentration temperature of 58-62° C. to an ethanol-free state to obtain an ethanol-free concentrated solution.

[0033] Preferably, the method further comprises step 5: heating the ultrafiltration effluent to 70-75° C., adding a preservative, continuing to heat the ultrafiltration effluent to 83-88° C., and collecting the effluent after keeping the temperature for 1 hour.

[0034] More preferably, the preservative is at least one of p-hydroxyacetophenone, 1,2-hexanediol, caprylhydroxyvaleric acid, 1,2-pentanediol, phenoxyethanol, and ethylhexylglycerin.

[0035] The invention also discloses use of an oil-controlling and anti-dandruff composition for preparing toiletries.

[0036] Meanwhile, the invention discloses a hair product containing 0.1-30wt% of the oil-controlling and anti-dandruff composition.

[0037] Beneficial Effects

[0038] Compared with the prior art, the present invention has at least the following advantages:

[0039] (1) The present invention discloses an oil-controlling and anti-dandruff composition, which produces a synergistic effect of improving the anti-dandruff and oil-control effects by compounding an extract of Channa intestine, an extract of Phyllanthus emblica, an extract of Safflower, and an extract of Lotus seed, and has excellent anti-dandruff and oil-control effects;

[0040] (2) The oil-controlling and anti-dandruff composition of the present invention can achieve excellent anti-dandruff and oil-control effects without requiring a large amount of active ingredients. BRIEF DESCRIPTION OF THE DRAWINGS

[0041] The present invention is further described below in conjunction with the accompanying drawings and embodiments;

[0042] Figure 1This is a chicken embryo observation picture before using the oil-controlling and anti-dandruff composition of Example 4 at a concentration of 2% of the present invention;

[0043] Figure 2 This is a picture of chicken embryos observed after using the oil-controlling and anti-dandruff composition of Example 4 at a concentration of 2% of the present invention. DETAILED DESCRIPTION

[0044] The present invention will be further described below in conjunction with the embodiments, but it does not constitute any limitation to the present invention. Any limited number of modifications made within the scope of the claims of the present invention are still within the scope of the claims of the present invention.

[0045] In order to explain the technical content of the present invention in detail, further description is given below in conjunction with the implementation modes.

[0046] In the following examples and comparative examples, unless otherwise specified, the Channa intestines, Phyllanthus emblica, safflower, lotus seeds, Sophora flavescens, rosemary, Hippophae rhamnoides, Panax notoginseng, Angelica sinensis, and Hypericum perforatum were all purchased from Bozhou Yuanshengtang Pharmaceutical Co., Ltd.;

[0047] It should be noted that the technical solution of the present invention is not limited to the raw materials obtained from the above sources, and the raw materials available on the market should also be included in the scope of the technical solution of the present invention.

[0048] In the following examples and comparative examples, unless otherwise specified, the parts and % refer to parts by weight and percentage by weight, respectively.

[0049] Example 1

[0050] An oil-controlling and anti-dandruff composition comprises the following components:

[0051] 6% of Channa intestine extract, 5% of Phyllanthus emblica extract, 3% of Carthamus tinctorius extract, 1% of Lotus seed extract, and the balance is water;

[0052] The extract of Channa intestine, Phyllanthus emblica extract, Safflower extract and Lotus seed extract are obtained by compound extraction through the following steps:

[0053] Step 1: Grind the chrysanthemum intestine, emblica fruit, safflower and lotus seeds into powder;

[0054] Step 2: adding water 10 times the weight of the powder to the powder, extracting with circulating water at an extraction temperature of 65° C. for 3 h to obtain an extract, then concentrating the extract to 20wt% of the mass of the extract through a single-effect concentrator at a concentration temperature of 65° C. to obtain a concentrated solution, adding 95% ethanol of the same mass as the concentrated solution to the concentrated solution, stirring for 1 h and then standing for 1 h to obtain an alcohol precipitate;

[0055] Step 3: centrifuging the alcohol precipitate to obtain a centrifuge, and then concentrating it at a concentration temperature of 60° C. to an ethanol-free state to obtain an ethanol-free concentrated solution;

[0056] Step 4: adding glycerol and water to the ethanol-free concentrated solution and stirring and mixing to obtain a mixed solution, wherein the mass percentage of the ethanol-free concentrated solution in the mixed solution is 20wt%, and the mass percentage of glycerol is 40wt%, and then transporting to an ultrafiltration device, the ultrafiltration membrane pore size is 0.1um, the ultrafiltration pressure is 0.30MPa, the ultrafiltration is turned on and the cooling circulating water is turned on, the ultrafiltration temperature is controlled at 40±2°C, and the ultrafiltration effluent is collected;

[0057] After the ultrafiltration effluent was heated to 72°C, an appropriate amount of p-hydroxyacetophenone was added, and the temperature was continued to rise to 88°C. After keeping the temperature for 1 hour, the effluent was collected.

[0058] Example 2

[0059] The method is substantially the same as Example 1, except that the oil-controlling and anti-dandruff composition comprises the following components:

[0060] 10% of Channa intestine extract, 2% of Phyllanthus emblica extract, 2% of Safflower extract, 0.1% of Lotus seed extract, and the balance is water.

[0061] Example 3

[0062] The method is substantially the same as Example 1, except that the oil-controlling and anti-dandruff composition comprises the following components:

[0063] 8% of Channa intestines extract, 6% of Phyllanthus emblica extract, 1% of Carthamus tinctorius extract, 3% of Lotus seed extract, and the balance is water.

[0064] Example 4

[0065] An oil-controlling and anti-dandruff composition comprises the following components:

[0066] 8% of Channa intestine extract, 4% of Phyllanthus emblica extract, 3.5% of Carthamus tinctorius extract, 0.5% of Lotus seed extract, 1% of Sophora flavescens root extract, and the balance is water;

[0067] The Channa intestine extract, Phyllanthus emblica extract, Safflower extract, Lotus seed extract, and Sophora flavescens root extract are obtained by composite extraction through the following steps:

[0068] Step 1: Grind the chrysanthemum intestine, emblica fruit, safflower, lotus seed and sophora flavescens root to obtain powder;

[0069] Step 2: adding water 10 times the weight of the powder to the powder, extracting with circulating water at an extraction temperature of 65° C. for 3 h to obtain an extract, then concentrating the extract to 20wt% of the mass of the extract through a single-effect concentrator at a concentration temperature of 65° C. to obtain a concentrated solution, adding 95% ethanol of the same mass as the concentrated solution to the concentrated solution, stirring for 1 h and then standing for 1 h to obtain an alcohol precipitate;

[0070] Step 3: centrifuging the alcohol precipitate to obtain a centrifuge, and then concentrating it at a concentration temperature of 60° C. to an ethanol-free state to obtain an ethanol-free concentrated solution;

[0071] Step 4: adding glycerol and water to the ethanol-free concentrated solution and stirring and mixing to obtain a mixed solution, wherein the mass percentage of the ethanol-free concentrated solution in the mixed solution is 20wt%, and the mass percentage of glycerol is 40wt%, and then transporting to an ultrafiltration device, the ultrafiltration membrane pore size is 0.1um, the ultrafiltration pressure is 0.30MPa, the ultrafiltration is turned on and the cooling circulating water is turned on, the ultrafiltration temperature is controlled at 40±2°C, and the ultrafiltration effluent is collected;

[0072] After the ultrafiltration effluent was heated to 72°C, an appropriate amount of p-hydroxyacetophenone was added, and the temperature was continued to rise to 88°C. After keeping the temperature for 1 hour, the effluent was collected.

[0073] Example 5

[0074] The method is substantially the same as Example 4, except that the oil control and anti-dandruff composition comprises the following components:

[0075] 6% of Channa Intestine Extract, 5% of Phyllanthus Emblica Extract, 3% of Carthamus Tinctorius Extract, 1% of Lotus Seed Extract, 0.1% of Sophora Flavescens Root Extract, and the balance is water.

[0076] Comparative Example 1

[0077] The method is substantially the same as Example 1, except that the oil-controlling and anti-dandruff composition comprises the following components:

[0078] Emblica extract 8.3%, safflower extract 5%, lotus seed extract 1.7%, the balance is water;

[0079] The composite extraction method is the same as in Example 1.

[0080] Comparative Example 2

[0081] The method is substantially the same as Example 1, except that the oil-controlling and anti-dandruff composition comprises the following components:

[0082] 9% of Channa intestine extract, 4.5% of safflower extract, 1.5% of lotus seed extract, and the balance is water;

[0083] The composite extraction method is the same as in Example 1.

[0084] Comparative Example 3

[0085] The method is substantially the same as Example 1, except that the oil-controlling and anti-dandruff composition comprises the following components:

[0086] 7.5% of Channa intestine extract, 6.25% of Phyllanthus emblica extract, 1.25% of lotus seed extract, and the balance is water;

[0087] The composite extraction method is the same as in Example 1.

[0088] Comparative Example 4

[0089] The method is substantially the same as Example 1, except that the oil-controlling and anti-dandruff composition comprises the following components:

[0090] 6.4% of Channa intestine extract, 5.3% of Phyllanthus emblica extract, 3.3% of Carthamus tinctorius extract, and the balance is water;

[0091] The composite extraction method is the same as in Example 1.

[0092] Comparative Example 5

[0093] The method is substantially the same as Example 1, except that the oil-controlling and anti-dandruff composition comprises the following components:

[0094] Rosemary extract 6%, emblica extract 5%, safflower extract 3%, lotus seed extract 1%, and the balance is water; the composite extraction method is the same as that in Example 1, except that the snakehead intestine is replaced by rosemary.

[0095] Comparative Example 6

[0096] The method is substantially the same as Example 1, except that the oil-controlling and anti-dandruff composition comprises the following components:

[0097] 6% of Channa intestine extract, 5% of Hippophae rhamnoides extract, 3% of Safflower extract, 1% of Lotus seed extract, and the balance is water; the composite extraction method is the same as that in Example 1, except that Phyllanthus emblica is replaced by Hippophae rhamnoides.

[0098] Comparative Example 7

[0099] The method is substantially the same as Example 1, except that the oil-controlling and anti-dandruff composition comprises the following components:

[0100] 6% of Channa intestine extract, 5% of Phyllanthus emblica extract, 3% of Panax notoginseng extract, 1% of lotus seed extract, and the balance is water; the composite extraction method is the same as that in Example 1, except that safflower is replaced by Panax notoginseng.

[0101] Comparative Example 8

[0102] An oil-controlling and anti-dandruff composition comprises the following components:

[0103] 6% of Channa intestine extract, 5% of Phyllanthus emblica extract, 3% of Carthamus tinctorius extract, 1% of Angelica sinensis extract, and the balance is water; the composite extraction method is the same as that in Example 1, except that lotus seeds are replaced with Angelica sinensis.

[0104] Comparative Example 9

[0105] An oil-controlling and anti-dandruff composition comprises the following components:

[0106] 8% of Channa intestine extract, 4% of Phyllanthus emblica extract, 3.5% of Carthamus tinctorius extract, 0.5% of Lotus seed extract, 1% of Hypericum perforatum extract, and the balance is water;

[0107] The composite extraction method is the same as that of Example 4, except that the root of Sophora flavescens is replaced with Hypericum perforatum.

[0108] Efficacy Test

[0109] 1. Anti-dandruff efficacy test (Malassezia furfur inhibition)

[0110] Test method:

[0111] 1 Experimental materials and methods

[0112] 1.1 Instruments and Equipment

[0113] Biochemical incubator

[0114] Vertical high pressure steam sterilizer

[0115] 1.2 Reagents

[0116] Phosphate buffered saline

[0117] Pityrosporum culture medium

[0118] Malassezia furfur

[0119] 2 Experimental methods

[0120] 2.1 Sample processing

[0121] Sample: Dilute with sterile water to a sample concentration of 0.5%

[0122] Blank control: phosphate buffer

[0123] Control sample group: Malassezia furfur suspension + phosphate buffer

[0124] 2.2 Experimental steps

[0125] Take the suspension of Malassezia furfur, add it to each sample solution and control solution, mix it evenly, start timing, and when the specified action time is reached, make appropriate dilutions. Take 2 to 3 dilutions, take the sample solution and control solution and place them on two plates respectively, pour the culture medium of Pityrosporum furfur and mix evenly, turn the plate over after it solidifies, put it in the incubator for 5 to 10 days, and count the live bacteria.

[0126] 3 Calculation formula

[0127]

[0128] Where: A—average colony count of control sample;

[0129] B—Average colony count of the tested samples.

[0130] Evaluation criteria: If the antibacterial rate is ≥50% to 90%, the product has antibacterial effect; if the antibacterial rate is ≥90%, the product has a strong antibacterial effect.

[0131] The oil-controlling and anti-dandruff compositions obtained in Examples 1-5 and Comparative Examples 1-9 were tested according to the above method, and the inhibition time was 10 minutes. The results are shown in Table 1;

[0132] Table 1 Antidandruff efficacy test results of the oil-control antidandruff compositions obtained in Examples 1-5 and Comparative Examples 1-9

[0133] Average colony count CFU / mL Malassezia furfur inhibition rate Example 1 <![CDATA[4.2*10 3 ]]> 73.8％ Example 2 <![CDATA[4.1*10 3 ]]> 74.4％ Example 3 <![CDATA[3.6*10 3 ]]> 77.5％ Example 4 <![CDATA[1.9*10 3 ]]> 88.1％ Example 5 <![CDATA[2.4*10 3 ]]> 85.0％ Comparative Example 1 <![CDATA[6.8*10 3 ]]> 57.5％ Comparative Example 2 <![CDATA[7.3*10 3 ]]> 54.4％ Comparative Example 3 <![CDATA[7.2*10 3 ]]> 55.0％ Comparative Example 4 <![CDATA[7*10 3 ]]> 56.3％ Comparative Example 5 <![CDATA[6.9*10 3 ]]> 56.9％ Comparative Example 6 <![CDATA[6.5*10 3 ]]> 59.4％ Comparative Example 7 <![CDATA[7.8*10 3 ]]> 51.3％ Comparative Example 8 <![CDATA[6.9*10 3 ]]> 56.9％ Comparative Example 9 <![CDATA[3.7*10 3 ]]> 76.9％ Control samples <![CDATA[1.6*10 4 ]]> / Blank control <10 /

[0134] According to the results in Table 1, we can see that:

[0135] According to the data of Examples 1-3, the composition obtained by compounding Channa intestine extract, Emblica emblica extract, Safflower extract and Lotus seed extract of the present invention has a very high Malassezia furfur inhibitory effect even at a low concentration of 0.5%, achieving a good anti-dandruff effect.

[0136] According to the data comparison of Examples 1-3 and 4, the addition of Sophora flavescens root extract on the basis of the compound of Channa intestine extract, Emblica emblica extract, Safflower extract and Lotus seed extract can improve the inhibitory effect of the composition on Malassezia furfur, indicating that Sophora flavescens root extract can synergistically improve the inhibitory ability of Malassezia furfur with Channa intestine extract, Emblica emblica extract, Safflower extract and Lotus seed extract.

[0137] According to the data comparison between Example 1 and Comparative Examples 1-4, although the inhibition rate of Malassezia furfur in Comparative Examples 1-4 exceeds 50%, the composition has an antibacterial effect; however, compared with Example 1, in the absence of any one of the extracts of Channa intestines extract, Emblica emblica extract, Carthamus tinctorius extract and Lotus seed extract, Comparative Examples 1-4 have a significant decrease in the inhibition effect of Malassezia furfur even if the dosage of other extracts is increased, indicating that the present invention uses the compound of Channa intestines extract, Emblica emblica extract, Carthamus tinctorius extract and Lotus seed extract to synergistically enhance the inhibition effect of Malassezia furfur.

[0138] Similarly, according to the data comparison between Example 1 and Comparative Examples 5-8, although Comparative Example 5 uses rosemary extract with similar efficacy to Channa intestine extract to replace Channa intestine extract, Comparative Example 6 uses seabuckthorn extract with similar efficacy to Phyllanthus emblica extract to replace Phyllanthus emblica extract, Comparative Example 7 uses Panax notoginseng extract with similar efficacy to Safflower extract to replace Safflower extract, and Comparative Example 8 uses Angelica extract to replace Lotus seed extract, but the Malassezia furfur inhibition rate shown by them is far different from that of Example 1, and is even equivalent to Comparative Examples 1-4, indicating that Comparative Examples 5-8 are similar to Comparative Examples 1-4 and can only produce a simple superposition of the effects between components, while the present invention produces a synergistic effect of enhancing the inhibition of Malassezia furfur by compounding Channa intestine extract, Phyllanthus emblica extract, Safflower extract and Lotus seed extract; especially Comparative Example 7, the inhibition rate of Malassezia furfur shown by it has a more obvious decrease, and it is even almost impossible to show the antibacterial effect. It is preliminarily judged that antagonism occurs between the components.

[0139] According to the data comparison of Example 4 and Comparative Example 9, it can be seen that the compounding of Hypericum perforatum extract with Channa chinensis extract, Phyllanthus emblica extract, Carthamus tinctorius extract and Lotus seed extract, which has similar efficacy to Sophora flavescens root extract, cannot effectively improve the inhibitory effect of Malassezia furfur. The Malassezia furfur inhibition rate is equivalent to that of Examples 1-3, indicating that the increase of 1% Hypericum perforatum extract does not produce a significant improvement in the anti-dandruff efficacy.

[0140] 2. Oil control efficacy test

[0141] Test method:

[0142] 1. Purpose and principle of the experiment

[0143] Oily skin has more active secretion of sebaceous glands, which makes the face feel greasy, difficult to clean, and has a glossy appearance that affects appearance.

[0144] At the same time, some corresponding skin diseases are also prone to occur, such as acne vulgaris and seborrheic dermatitis. In view of the relationship between 5α-reductase and sebaceous glands, the inhibitory effect of the test sample on 5α-reductase is usually tested to reflect the sebum regulation effect, so as to characterize the oil control effect of the test sample.

[0145] This test refers to the laboratory method and compares the 5α-reductase inhibition rate test results of the test sample with that of the negative control. If the inhibition rate of the test sample is higher than that of the negative control and there is a significant difference (P<0.05), it can be considered that the test sample has a certain oil control effect.

[0146] This test method is an in vitro method and is suitable for evaluating cosmetic products that claim to achieve oil control by inhibiting 5α-reductase.

[0147] 2 Test indicators

[0148] 5α-reductase inhibition rate determination standard: If the 5α-reductase inhibition rate of the sample is higher than that of the negative control and there is a significant difference (P<0.05), it can be considered that the test sample has a certain oil control effect.

[0149] 3 Experimental materials and methods

[0150] Testosterone, 98%

[0151] NADPH, 90%

[0152] Methanol, HPLC grade

[0153] Buffer: Tris-HCl

[0154] 3.3 Test methods

[0155] (1) Treatment of control and test samples

[0156] Sample group: diluted with buffer to a sample concentration of 2%;

[0157] Positive control (finasteride, purity ≥98%): dilute with methanol to a positive control concentration of 0.1%;

[0158] Negative control: Tris-HCl buffer.

[0159] (2) Test operation steps

[0160] A sample group, a positive control group, a negative control group and a blank group were set up. Three parallels were set up for each group. Different reagent solutions were added to the four groups respectively, and the mixture was shaken well. Each group was subjected to HPLC analysis to determine the testosterone content in each tube.

[0161] (3) Calculation formula

[0162]

[0163] (4) Data analysis

[0164] The statistical analysis software was SPSS, and the comparison of 5α-reductase inhibition rates among the test samples, positive controls, and negative controls was performed using an independent sample t-test. The above statistical analyses were all two-tailed tests, with a significance level of α = 0.05. P>0.05 indicated that there was no significant difference between the two groups; P<0.05 indicated that there was a significant difference between the two groups.

[0165] The oil-controlling and anti-dandruff compositions obtained in Examples 1-5 and Comparative Examples 1-9 were tested according to the above method, and the results are shown in Table 2;

[0166] Table 2 Test results of oil control efficacy of the oil control and anti-dandruff compositions obtained from Examples 1-5 and Comparative Examples 1-9 at 2% concentration

[0167]

[0168]

[0169] According to the results in Table 2:

[0170] According to the data of Examples 1-3, the composition obtained by compounding Channa intestine extract, Phyllanthus emblica extract, Safflower extract and Lotus seed extract in the present invention showed a very high 5α-reductase inhibition effect at a concentration of 2%, achieving excellent oil control effect.

[0171] According to the data comparison of Examples 1-3 and 4, it can be seen that the addition of Sophora flavescens root extract on the basis of the compound of Channa intestine extract, Emblica emblica extract, Safflower extract and Lotus seed extract can improve the 5α-reductase inhibitory effect of the composition, indicating that Sophora flavescens root extract can synergistically improve the 5α-reductase inhibitory ability with Channa intestine extract, Emblica emblica extract, Safflower extract and Lotus seed extract.

[0172] According to the data comparison between Example 1 and Comparative Examples 1-4, compared with Example 1, in the absence of any one of the extracts of Channa Intestines Extract, Emblica Extract, Safflower Extract and Lotus Seed Extract, Comparative Examples 1-4 have a significantly decreased 5α-reductase inhibitory effect even if the dosage of other extracts is increased, indicating that the present invention uses a compound of Channa Intestines Extract, Emblica Extract, Safflower Extract and Lotus Seed Extract to synergistically enhance the 5α-reductase inhibitory effect.

[0173] Similarly, according to the data comparison between Example 1 and Comparative Examples 5-8, although Comparative Example 5 uses rosemary extract with similar efficacy to Channa Intestine Extract to replace Channa Intestine Extract, Comparative Example 6 uses Hippophae Rhamnoides Extract with similar efficacy to Phyllanthus Emblica Extract to replace Phyllanthus Emblica Extract, Comparative Example 7 uses Panax Notoginseng Extract with similar efficacy to Safflower Extract to replace Safflower Extract, and Comparative Example 8 uses Angelica Sinensis Extract to replace Lotus Seed Extract, but the 5α-reductase inhibition rate exhibited by the composition at a concentration of 2% is significantly different from that of Example 1, and is even equivalent to that of Comparative Examples 1-4, indicating that Comparative Examples 5-8 are similar to Comparative Examples 1-4 and can only produce a simple superposition of the efficacy of the components, while the present invention produces a synergistic effect of enhancing the 5α-reductase inhibition effect by compounding Channa Intestine Extract, Phyllanthus Emblica Extract, Safflower Extract and Lotus Seed Extract.

[0174] However, according to the data comparison of Example 4 and Comparative Example 9, the combination of Hypericum perforatum extract with Channa chebula extract, Phyllanthus emblica extract, Carthamus tinctorius extract and Lotus seed extract, which has similar efficacy to Sophora flavescens root extract, cannot effectively improve the 5α-reductase inhibition effect.

[0175] 3. Irritation test

[0176] Test Method

[0177] 1. Purpose and principle of the experiment

[0178] The chicken embryo chorioallantoic membrane test is an early in vitro eye irritation assessment method. The chorioallantoic membrane (CAM) is a respiratory membrane that surrounds the chicken embryo. This test uses the characteristics of the intact, clear and transparent vascular system of the chorioallantoic membrane of the mid-stage incubated chicken embryo. A certain amount of the test substance is directly exposed to the chicken embryo chorioallantoic membrane. After a period of action, the changes in the toxic effect indicators of the chorioallantoic membrane (such as bleeding, coagulation and vascular melting) are observed. These indicators reflect the changes in the morphological structure, color and permeability of the blood vessels and vascular networks, as well as the phenomena such as the denaturation of the chorioallantoic membrane proteins and the degree of damage. Then, a score is obtained by combining them to evaluate the eye irritation of the test substance.

[0179] The purpose of this test is to test the ability of the test substance to cause toxic changes in the chicken embryo chorioallantoic membrane and to evaluate the elements and processes of the potential eye irritation of the evaluated substance.

[0180] This test method is an in vitro method and is suitable for the detection of cosmetics and cosmetic raw materials.

[0181] 2 Test indicators

[0182] CAMVA irritation test: ES≤4, non-irritating; 4<ES≤12, mild irritating; 12<ES<16, moderate irritating; ES≥16, strong irritating / corrosive.

[0183] 3 Experimental materials and methods

[0184] 3.1 Instruments and Equipment

[0185] Fully automatic incubator

[0186] Stereo microscope

[0187] SPF Chicken Embryo

[0188] 3.2 Reagents

[0189] Sodium chloride

[0190] Sodium dodecyl sulfate (SDS)

[0191] 3.3 Incubation conditions

[0192] Room temperature 20℃~25℃, relative humidity 45%~70%. Incubation temperature 37.5℃±0.5℃, relative humidity 55%~70%, turntable 3 times / h~6 times / h. Rotation is not necessary when incubating 9-day-old chick embryos.

[0193] 3.4 Test methods

[0194] (1) Test operation steps

[0195] In this test, 6 embryos were selected for each group, and the conditions of the chorioallantoic membrane were recorded with a camera. A polytetrafluoroethylene resin ring was placed on the chorioallantoic membrane of the chicken embryo and photographed. The sample to be tested was added to the polytetrafluoroethylene resin ring, the time of adding the sample was recorded, and the air chamber was covered with a moistened plastic wrap. The chicken embryo was moved to a constant temperature and humidity chamber for culture, and the degree of change of each toxic effect was observed.

[0196] (2) Observation of results

[0197] Bleeding, coagulation, and vascular melting were observed and recorded, and scored according to their severity.

[0198] (3) Data analysis

[0199] Table 3 Scoring criteria

[0200]

[0201] The test was carried out using the endpoint evaluation method, and the endpoint evaluation (ES) was calculated with the result retained to two decimal places; the score of each chicken embryo = the sum of the bleeding, coagulation and vascular melting observed in each chicken embryo; ES = the average of the mathematical sums obtained for 6 chicken embryos.

[0202] According to the above test method, Examples 1-5 and Comparative Examples 1-9 were diluted into 2% aqueous solutions and tested for samples. The results showed that they were non-irritating.

[0203] Wherein, the chicken embryo result of embodiment 4 is as follows Figure 1-Figure 2 As shown, Figure 1 This is a chicken embryo observation picture before using the sample of Example 4. Figure 2 This is a chicken embryo observation diagram after using the sample of Example 4. The test result of the sample of Example 4 is ES=2.33;

[0204] This proves that the technical solution of the present invention is mild and non-irritating.

[0205] Application Examples

[0206] A shampoo is prepared according to the formula table in Table 4, and the preparation method is as follows:

[0207] Step 1: Add water to a homogenizing pot, add hydroxypropyl guar hydroxypropyl trimethyl ammonium chloride under homogenization, homogenize and disperse evenly, heat to 65°C under stirring, add anhydrous citric acid, and then continue to heat to 85°C under stirring, keep warm and stir for 15-20 minutes to obtain phase A;

[0208] Step 2: Maintain 85°C, add Phase B ingredients, and stir until completely dissolved;

[0209] Step 3: Cool down to 45°C, add Phase C raw materials, and disperse and dissolve evenly to obtain shampoo.

[0210] Table 4 Basic shampoo formula

[0211]

[0212]

[0213] The embodiments presented herein are only embodiments selected according to the combination of all possible embodiments. The appended claims should not be limited by the embodiments describing the present invention. Some numerical ranges used in the claims include sub-ranges therein, and the changes in these ranges should also be covered by the appended claims.

Claims

1. An oil-controlling and anti-dandruff composition, characterized in that: By weight percentage, it includes the following components: 6-10% of Channa intestine extract, 2-6% of Phyllanthus emblica extract, 1-5% of Safflower extract, 0.1-3% of Lotus seed extract, and 0.1-3% of Sophora flavescens root extract; the Channa intestine extract, Phyllanthus emblica extract, Safflower extract, Lotus seed extract, and Sophora flavescens root extract are obtained by composite extraction through the following steps: Step 1: Grind the chrysanthemum intestine, emblica fruit, safflower, lotus seed and sophora flavescens root to obtain powder; Step 2: add 10 times the weight of the powder into the powder, extract with circulating water at an extraction temperature of 65°C for 3 hours to obtain an extract, then concentrate the extract through a single-effect concentrator at a concentration temperature of 65°C to 20wt% of the extract mass to obtain a concentrated solution, add 95% ethanol of the same mass as the concentrated solution to the concentrated solution, stir for 1 hour, and then stand for 1 hour to obtain an alcohol precipitate; Step 3: centrifuging the alcohol precipitate to obtain a centrifuge, and then concentrating it at a concentration temperature of 60° C. to an ethanol-free state to obtain an ethanol-free concentrated solution; Step 4: adding glycerol and water to the ethanol-free concentrated solution and stirring to obtain a mixed solution, wherein the mass percentage of the ethanol-free concentrated solution in the mixed solution is 15-20wt% and the mass percentage of glycerol is 20-50wt%, and then transporting to an ultrafiltration device, the ultrafiltration membrane pore size is 0.1um, the ultrafiltration pressure is 0.30MPa, the ultrafiltration is turned on and the cooling circulating water is turned on, the ultrafiltration temperature is controlled at 40±2°C, and the ultrafiltration effluent is collected; After the ultrafiltration effluent was heated to 72°C, an appropriate amount of preservative was added, and the temperature was continued to rise to 88°C, and the mixture was collected after being kept warm for 1 hour; The preservative is at least one of p-hydroxyacetophenone, 1,2-hexanediol, 1,2-pentanediol, phenoxyethanol, and ethylhexylglycerin.

2. Use of the oil-controlling and anti-dandruff composition according to claim 1 in preparing toiletries.

3. A hair product, characterized in that: Contains 0.1-30wt% of the oil-controlling and anti-dandruff composition according to claim 1.

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