Multi-effect microbial organic compound fertilizer as well as preparation method and application thereof

Abstract

The invention discloses a multi-effect microbial organic compound fertilizer and a preparation method and application thereof, compared with a traditional organic fertilizer or a bio-organic fertilizer, the compound microbial agent provided by the invention is extracted from plant rhizosphere, has the functions of growth promotion and yield increase, immune induced resistance, stress adaptation and resistance and the like, and can be applied to large-scale fermentation production of the multi-effect microbial organic compound fertilizer. The compound is compounded into a fermented and decomposed organic fertilizer, is applied to commercial crops or field crops, and can achieve the effects of promoting crop growth, resisting diseases and the like; according to the invention, through combined use of the hyphae rhamnoides and the fusarium oxysporum, pathogenic bacteria can be inhibited, and root growth and nutrient absorption capability of crops can be enhanced at the same time; the combination of pseudobottle oryzae and piriformospora indica can improve the disease resistance of crops, promote the growth of the crops and increase the yield of the crops; through the synergistic effect of multiple strains, the yield and quality of crops can be improved, soil health can be effectively improved, and sustainable development of green agriculture is promoted.

Description

Technical Field

[0001] The present invention relates to the technical field of agricultural fertilizers, and in particular to a multi-functional microbial organic compound fertilizer and a preparation method and application thereof. Background Art

[0002] Fertilization is an important management measure to increase crop yields in agricultural production and is very important for maintaining farmland soil productivity. Fertilizers play an important supporting role in ensuring crop yields and stabilizing agricultural production, but their use also brings serious environmental pollution problems, such as eutrophication of soil and water bodies, which in turn hinders the sustainable development of agriculture.

[0003] As an alternative to chemical fertilizers, organic fertilizers are gradually gaining attention due to their green, healthy and less polluting characteristics. Organic fertilizers can effectively improve soil structure, increase soil organic matter content, and provide essential nutrients such as nitrogen, phosphorus, and potassium, thereby promoting the healthy growth of crops. However, while organic fertilizers bring benefits, they also face the problem of high heavy metal content, which not only threatens soil quality but may also affect the safety of agricultural products. Although my country's organic fertilizer industry has developed rapidly, a complete organic fertilizer quality evaluation system has not yet been established, resulting in some organic fertilizer products being substandard, further affecting farmers' confidence in use and market stability.

[0004] In this context, it is particularly urgent to accelerate the research and development of green agricultural inputs. As a new type of fertilizer, microbial organic fertilizer can effectively combine the advantages of organic fertilizer and biotechnology. By adding a certain number of functional microorganisms, it can inhibit the toxicity of pathogens in the soil, improve the stress resistance of crops, promote crop growth, and improve the quality and yield of agricultural products. These functional microorganisms can not only enhance the biological activity of the soil, but also promote the effective release of nutrients, thereby enhancing the growth potential of crops.

[0005] However, the current bio-organic fertilizer products in my country are still relatively simple in terms of microbial strain resources, with the most widely used Bacillus subtilis accounting for 61.6% of the registered products (including composite strain products). Many bio-organic fertilizer companies lack a deep understanding of the sources of microbial strains, and are not clear about the safety of microorganisms, which increases the risk of ecological and environmental safety. In addition, the overall level of the bio-organic fertilizer industry is not high, technological innovation is insufficient, strains and products are highly homogenized, and product quality and application effects are unstable, which need to be solved urgently.

[0006] For example, Chinese patent document CN202310075315.1 discloses a microbial organic fertilizer and a preparation method thereof, wherein the microbial organic fertilizer is composed of fermented organic fertilizer and slow-release nitrogen fertilizer; the microbial organic fertilizer includes the following components in parts by weight: 35-45 parts of chicken manure, 20-30 parts of corn flour, 25-40 parts of straw powder, 10-15 parts of rice bran, 10-20 parts of composite microbial agent, and 20-23 parts of water; the composite microbial agent is composed of a Bacillus subtilis suspension, a Trichoderma harzianum suspension, and a Bacillus megaterium suspension; the slow-release nitrogen fertilizer is obtained by polymerization of acrylic acid, acrylamide, bentonite, and urea; through the above technical scheme, the problem of large-scale application of chemical fertilizers in the prior art leading to a decrease in crop quality and yield is solved, but the technical problem of serious homogeneity of strains and products is still not solved. Summary of the invention

[0007] In view of the deficiencies in the prior art, the object of the present invention is to provide a multi-functional microbial organic compound fertilizer and a preparation method and application thereof.

[0008] In order to achieve the above object, the present invention adopts the following technical scheme:

[0009] In a first aspect, the present invention provides a multi-functional microbial organic compound fertilizer, which is composed of fermented organic fertilizer and a composite microbial agent, wherein the composite microbial agent is composed of a suspension of Giant Aspergillus oryzae, a suspension of Sickle bottle mold of rice, a suspension of Pseudomonas oryzae, and a suspension of Indian Pear-shaped spores.

[0010] Specifically, the mass ratio of the fermented organic fertilizer and the composite microbial agent is 1000g:4-8mL. In some embodiments of the present invention, for example, 1000g:4mL, 1000g:5mL, 1000g:6mL, 1000g:7mL, 1000g:8mL can be selected, but it is not limited to the listed values, and other unlisted values ​​within the numerical range are also applicable.

[0011] The preparation method of the fermented organic fertilizer is as follows: sheep manure, chicken manure and straw powder are mixed and fermented to obtain the organic fertilizer.

[0012] More specifically, the preparation method of the fermented organic fertilizer is as follows: after mixing the raw materials of sheep manure, chicken manure and crushed straw according to the mass ratio, adjusting the initial organic matter content of the mixed material to 50%-60%, the initial water content to 50-40%, and the carbon-nitrogen ratio to 25-30:1, using a Dano-type fermentation drum for high-temperature aerobic fermentation, the fermentation tank is filled to 0.5-0.75, and the air supply volume is 0.2m 3 / min / m 3The fermentation tank rotates at 10r / h. During the fermentation period, the temperature inside the pile is observed by a temperature sensor every day. When the temperature at the core of the pile reaches above 50°C, the rotation speed is increased to 20-30r / h. After 3-5 days of continuous fermentation, the temperature of the pile begins to drop, the stench and flies are reduced, and the material can be discharged. Then, the material is fermented in an open aging workshop for 15-20 days to naturally reduce the temperature of the pile, and gradually stop emitting stench and attracting mosquitoes and flies. The pile is 3-5m wide and 2-3m high, with a triangular cross-section. It is turned over every 2 days, and then the fermented semi-finished fertilizer is passed through a drying equipment to reduce its water content to below 30%, thus obtaining fermented organic fertilizer.

[0013] Among them, the mass ratio of the sheep manure, chicken manure and straw powder is 2-4:1-2:1-2. In some embodiments of the present invention, for example, 2:1:1, 2:1:2, 2:2:2, 3:1:1, 3:1:2, 4:1:1, 4:2:1, 4:2:2 can be selected, but it is not limited to the listed values, and other unlisted values ​​within the numerical range are also applicable.

[0014] Specifically, the volume ratio of the suspension of Giant Soil Porphyra, the suspension of Sickle Bottle of Rice, the suspension of Pseudomonas oryzae and the suspension of Indian Pear-Shaped Spore in the composite microbial inoculant is 1-2:1-2:1-2:1-2. In some embodiments of the present invention, for example, 1:1:1:1, 1:2:2:1, 1:2:1:2, 2:1:1:1, 2:1:2:1, 2:2:1:1, 2:1:1:2 can be selected, but it is not limited to the listed values, and other values ​​not listed within the numerical range are also applicable.

[0015] Specifically, the number of viable bacteria in the suspension of Giant Soil Fungus, the suspension of Falcaria oryzae, the suspension of Pseudomonas oryzae and the suspension of Piriformis indica is not less than 1.0×10 9 cfu / mL.

[0016] More specifically, the culture method of the above-mentioned bacterial suspension is as follows: a liquid fermentation medium is prepared according to the ratio of 3-5 g / L glucose, 3-5 g / L soybean cake powder, 8-12 g / L corn flour, 0.3-0.5 g / L peptone, 0.5-1 g / L yeast powder, 0.5-1.5 g / L rice straw extract, 0.5-1 g / L potassium dihydrogen phosphate and 0.3-0.5 g / L magnesium sulfate, and the medium is cooled to room temperature after steam sterilization, and seed solutions of giant shell fungus, sickle bottle mold of rice, false bottle mold of rice, and Indian pear-shaped spore sorrel are inoculated at a ratio of 4‰ (v / v), the ventilation of the fermentation tank is maintained at 1:0.4, the tank pressure is maintained at 0.05 MPa, stirring is performed once every 3 hours, and fermentation is carried out for 12 days to obtain a bacterial suspension.

[0017] In a second aspect, the present invention provides a method for preparing the above-mentioned multi-functional microbial organic compound fertilizer, comprising the following steps: conveying the fermented organic fertilizer from a collecting conveyor to a mixing and crushing machine, spraying a composite microbial agent, and then packaging it through an automatic packaging machine to obtain a multi-functional microbial organic compound fertilizer.

[0018] Specifically, the mesh size of the mixing and pulverizing machine is 60-80 meshes.

[0019] In a third aspect, the present invention also provides the application of the above-mentioned multi-functional microbial organic compound fertilizer in agricultural production.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] (1) Compared with traditional organic fertilizers or biological organic fertilizers, the composite microbial agent provided by the present invention is extracted from the rhizosphere of plants and has the functions of promoting growth and increasing production, inducing immunity, and adapting to stress and resisting stress. After large-scale fermentation production, it is compounded into fermented and decomposed organic fertilizers to create microbial organic compound fertilizers with the functions of promoting production, preventing diseases, and repairing soil. When applied to economic crops or field crops, it can promote crop growth and resist the occurrence of diseases.

[0022] (2) The crust-like giant shell fungus plays an important role in the cultivation of crops. It can not only inhibit the growth of soil pathogens, but also improve their resistance to diseases and enhance their adaptability to environmental stress; the rice sickle bottle mold can effectively absorb heavy metals and harmful substances in the soil, reducing their negative impact on crop growth. At the same time, it can interact with the crop root system, promote the development of the crop root system and nutrient absorption, thereby increasing crop yields; the rice pseudophial mold can enhance the disease resistance of crops, especially resistance to fungal diseases; the Indian pear-shaped spore promotes nutrient absorption and utilization efficiency by forming a symbiotic relationship with the crop root system.

[0023] (3) There is a significant synergistic effect between the strains provided by the present invention; the combined use of Giant Soil Fungus and Falcaria oryzae can inhibit pathogens while enhancing the root growth and nutrient absorption capacity of crops; the combination of Pseudomonas oryzae and Pyriformis indica can improve the disease resistance of crops while promoting their growth and increasing crop yields; the present invention can not only improve the yield and quality of crops through the synergistic effect between multiple strains, but also effectively improve soil health and promote the sustainable development of green agriculture. DETAILED DESCRIPTION

[0024] The present invention is further described in detail below through specific preferred embodiments, but the present invention is not limited to the following embodiments.

[0025] It should be noted that, unless otherwise specified, the chemical reagents involved in the present invention were purchased through commercial channels.

[0026] The present invention provides the giant shell fungus M-B927, with a deposit number of CCTCC M 2021503, from Zhejiang University;

[0027] F. oryzae FO-R20, with the accession number CCTCC M 2021505, was obtained from Zhejiang University;

[0028] Pseudomonas oryzae P-B313, with the accession number CCTCC M 2021504, was obtained from Zhejiang University;

[0029] Piriformospora indica was kindly donated by Zhejiang University;

[0030] The straw powder is wheat straw powder with a mesh size of 80 meshes.

[0031] Example 1

[0032] A method for preparing a multi-functional microbial organic compound fertilizer comprises the following steps:

[0033] The fermented organic fertilizer is conveyed to a mixing and crushing machine by a collecting conveyor, the mesh number of the crushing is 80 meshes, and the composite microbial agent is sprayed at the same time, and the dosage ratio of the fermented organic fertilizer and the composite microbial agent is 1000g:6mL, and then it is packaged by an automatic packaging machine to obtain a multi-effect microbial organic compound fertilizer;

[0034] The preparation method of fermented organic fertilizer is as follows: the raw materials of sheep manure, chicken manure and crushed straw are mixed in a mass ratio of 3:1:1, and the initial organic matter content of the mixed material is adjusted to 50%, the initial water content is 50%, and the carbon-nitrogen ratio is 25:1. The Dano-type fermentation drum is used for high-temperature aerobic fermentation, the fermentation tank is filled to 0.5, and the air supply volume is 0.2m 3 / min / m 3 The fermentation tank rotates at a speed of 10r / h. During the fermentation period, the temperature inside the pile is observed by a temperature sensor every day. When the temperature at the core of the pile reaches above 50°C, the rotation speed is increased to 20r / h. After 3 days of continuous fermentation, the temperature of the pile begins to drop, the stench and fly bites are reduced, and the material can be discharged. Subsequently, the material is fermented in an open aging workshop for 15 days to naturally reduce the temperature of the pile, and gradually stop emitting stench and attracting mosquitoes and flies. The pile is 5m wide and 3m high, with a triangular cross-section. It is turned over once every 2 days, and then the fermented semi-finished fertilizer is passed through a drying device to reduce its moisture content to 20%, thus obtaining fermented organic fertilizer.

[0035] The volume ratio of the suspension of Giant Aspergillus oryzae, the suspension of Fusarium oryzae, the suspension of Pseudomonas oryzae and the suspension of Piriformis indica in the composite microbial inoculant is 1:1:1:1;

[0036] The number of viable bacteria in the suspensions of Giant Aspergillus shellii, Fusarium oryzae, Pseudomonas oryzae and Piriformis indica were all 1.0×10 10 cfu / mL.

[0037] Example 2

[0038] A method for preparing a multi-functional microbial organic compound fertilizer comprises the following steps:

[0039] The fermented organic fertilizer is conveyed to a mixing and crushing machine by a collecting conveyor, the mesh number of the crushing is 80 meshes, and the composite microbial agent is sprayed at the same time, and the dosage ratio of the fermented organic fertilizer and the composite microbial agent is 1000g:4mL, and then it is packaged by an automatic packaging machine to obtain a multi-effect microbial organic compound fertilizer;

[0040] The preparation method of fermented organic fertilizer is as follows: the raw materials of sheep manure, chicken manure and crushed straw are mixed in a mass ratio of 4:2:2, and the initial organic matter content of the mixed material is adjusted to 50%, the initial water content is 50%, and the carbon-nitrogen ratio is 25:1. The Dano-type fermentation drum is used for high-temperature aerobic fermentation, the fermentation tank is filled to 0.5, and the air supply volume is 0.2m 3 / min / m 3 The fermentation tank rotates at a speed of 10r / h. During the fermentation period, the temperature inside the pile is observed by a temperature sensor every day. When the temperature at the core of the pile reaches above 50°C, the rotation speed is increased to 20r / h. After 3 days of continuous fermentation, the temperature of the pile begins to drop, the stench and fly bites are reduced, and the material can be discharged. Subsequently, the material is fermented in an open aging workshop for 15 days to naturally reduce the temperature of the pile, and gradually stop emitting stench and attracting mosquitoes and flies. The pile is 5m wide and 3m high, with a triangular cross-section. It is turned over once every 2 days, and then the fermented semi-finished fertilizer is passed through a drying device to reduce its moisture content to 20%, thus obtaining fermented organic fertilizer.

[0041] The volume ratio of the suspension of Giant Aspergillus oryzae, the suspension of Fusarium oryzae, the suspension of Pseudomonas oryzae and the suspension of Piriformis indica in the composite microbial inoculant is 1:1:1:1;

[0042] The number of viable bacteria in the suspensions of Giant Aspergillus shellii, Fusarium oryzae, Pseudomonas oryzae and Piriformis indica were all 1.0×10 10 cfu / mL.

[0043] Example 3

[0044] A method for preparing a multi-functional microbial organic compound fertilizer comprises the following steps:

[0045] The fermented organic fertilizer is conveyed to a mixing and crushing machine by a collecting conveyor, the mesh number of the crushing is 80 meshes, and the composite microbial agent is sprayed at the same time, and the dosage ratio of the fermented organic fertilizer and the composite microbial agent is 1000g:8mL, and then it is packaged by an automatic packaging machine to obtain a multi-effect microbial organic compound fertilizer;

[0046] The preparation method of fermented organic fertilizer is as follows: the raw materials of sheep manure, chicken manure and crushed straw are mixed in a mass ratio of 2:2:2, and the initial organic matter content of the mixed material is adjusted to 50%, the initial water content is 50%, and the carbon-nitrogen ratio is 25:1. The Dano-type fermentation drum is used for high-temperature aerobic fermentation, the fermentation tank is filled to 0.5, and the air supply volume is 0.2m 3 / min / m 3 The fermentation tank rotates at a speed of 10r / h. During the fermentation period, the temperature inside the pile is observed by a temperature sensor every day. When the temperature at the core of the pile reaches above 50°C, the rotation speed is increased to 20r / h. After 3 days of continuous fermentation, the temperature of the pile begins to drop, the stench and fly bites are reduced, and the material can be discharged. Subsequently, the material is fermented in an open aging workshop for 15 days to naturally reduce the temperature of the pile, and gradually stop emitting stench and attracting mosquitoes and flies. The pile is 5m wide and 3m high, with a triangular cross-section. It is turned over once every 2 days, and then the fermented semi-finished fertilizer is passed through a drying device to reduce its moisture content to 20%, thus obtaining fermented organic fertilizer.

[0047] The volume ratio of the suspension of Giant Aspergillus oryzae, the suspension of Fusarium oryzae, the suspension of Pseudomonas oryzae and the suspension of Piriformis indica in the composite microbial inoculant is 2:1:2:1;

[0048] The number of viable bacteria in the suspensions of Giant Aspergillus shellii, Fusarium oryzae, Pseudomonas oryzae and Piriformis indica were all 1.0×10 10 cfu / mL.

[0049] Example 4

[0050] A method for preparing a multi-functional microbial organic compound fertilizer comprises the following steps:

[0051] The fermented organic fertilizer is conveyed to a mixing and crushing machine by a collecting conveyor, the mesh number of the crushing is 80 meshes, and the composite microbial agent is sprayed at the same time, and the dosage ratio of the fermented organic fertilizer and the composite microbial agent is 1000g:5mL, and then it is packaged by an automatic packaging machine to obtain a multi-effect microbial organic compound fertilizer;

[0052] The preparation method of fermented organic fertilizer is as follows: the raw materials of sheep manure, chicken manure and crushed straw are mixed in a mass ratio of 3:2:2, and the initial organic matter content of the mixed material is adjusted to 50%, the initial water content is 50%, and the carbon-nitrogen ratio is 25:1. The Dano-type fermentation drum is used for high-temperature aerobic fermentation, the fermentation tank is filled to 0.5, and the air supply volume is 0.2m 3 / min / m3 The fermentation tank rotates at a speed of 10r / h. During the fermentation period, the temperature inside the pile is observed by a temperature sensor every day. When the temperature at the core of the pile reaches above 50°C, the rotation speed is increased to 20r / h. After 3 days of continuous fermentation, the temperature of the pile begins to drop, the stench and fly bites are reduced, and the material can be discharged. Subsequently, the material is fermented in an open aging workshop for 15 days to naturally reduce the temperature of the pile, and gradually stop emitting stench and attracting mosquitoes and flies. The pile is 5m wide and 3m high, with a triangular cross-section. It is turned over once every 2 days, and then the fermented semi-finished fertilizer is passed through a drying device to reduce its moisture content to 20%, thus obtaining fermented organic fertilizer.

[0053] The volume ratio of the suspension of Giant Aspergillus oryzae, the suspension of Fusarium oryzae, the suspension of Pseudomonas oryzae and the suspension of Piriformis indica in the composite microbial inoculant is 2:1:1:2;

[0054] The number of viable bacteria in the suspensions of Giant Aspergillus shellii, Fusarium oryzae, Pseudomonas oryzae and Piriformis indica were all 1.0×10 10 cfu / mL.

[0055] Comparative Example 1

[0056] A method for preparing a multi-functional microbial organic compound fertilizer comprises the following steps:

[0057] The fermented organic fertilizer is conveyed to a mixing and crushing machine by a collecting conveyor, the mesh number of the crushing is 80 meshes, and the composite microbial agent is sprayed at the same time, and the dosage ratio of the fermented organic fertilizer and the composite microbial agent is 1000g:6mL, and then it is packaged by an automatic packaging machine to obtain a multi-effect microbial organic compound fertilizer;

[0058] The preparation method of fermented organic fertilizer is as follows: the raw materials of sheep manure, chicken manure and crushed straw are mixed in a mass ratio of 3:1:1, and the initial organic matter content of the mixed material is adjusted to 50%, the initial water content is 50%, and the carbon-nitrogen ratio is 25:1. The Dano-type fermentation drum is used for high-temperature aerobic fermentation, the fermentation tank is filled to 0.5, and the air supply volume is 0.2m 3 / min / m 3 The fermentation tank rotates at a speed of 10r / h. During the fermentation period, the temperature inside the pile is observed by a temperature sensor every day. When the temperature at the core of the pile reaches above 50°C, the rotation speed is increased to 20r / h. After 3 days of continuous fermentation, the temperature of the pile begins to drop, the stench and fly bites are reduced, and the material can be discharged. Subsequently, the material is fermented in an open aging workshop for 15 days to naturally reduce the temperature of the pile, and gradually stop emitting stench and attracting mosquitoes and flies. The pile is 5m wide and 3m high, with a triangular cross-section. It is turned over once every 2 days, and then the fermented semi-finished fertilizer is passed through a drying device to reduce its moisture content to 20%, thus obtaining fermented organic fertilizer.

[0059] The volume ratio of the suspension of Giant Aspergillus oryzae and the suspension of Falcaria oryzae in the composite microbial agent is 1:1;

[0060] The viable counts of the suspensions of Giant Aspergillus shell and Fusarium oryzae were both 1.0×10 10 cfu / mL.

[0061] Compared with Example 1, Comparative Example 1 shows that the composite microbial agent consists of a suspension of Macrocystis sphaerocephala and a suspension of Falcaria oryzae.

[0062] Comparative Example 2

[0063] A method for preparing a multi-functional microbial organic compound fertilizer comprises the following steps:

[0064] The fermented organic fertilizer is conveyed to a mixing and crushing machine by a collecting conveyor, the mesh number of the crushing is 80 meshes, and the composite microbial agent is sprayed at the same time, and the dosage ratio of the fermented organic fertilizer and the composite microbial agent is 1000g:6mL, and then it is packaged by an automatic packaging machine to obtain a multi-effect microbial organic compound fertilizer;

[0065] The preparation method of fermented organic fertilizer is as follows: the raw materials of sheep manure, chicken manure and crushed straw are mixed in a mass ratio of 3:1:1, and the initial organic matter content of the mixed material is adjusted to 50%, the initial water content is 50%, and the carbon-nitrogen ratio is 25:1. The Dano-type fermentation drum is used for high-temperature aerobic fermentation, the fermentation tank is filled to 0.5, and the air supply volume is 0.2m 3 / min / m 3 The fermentation tank rotates at a speed of 10r / h. During the fermentation period, the temperature inside the pile is observed by a temperature sensor every day. When the temperature at the core of the pile reaches above 50°C, the rotation speed is increased to 20r / h. After 3 days of continuous fermentation, the temperature of the pile begins to drop, the stench and fly bites are reduced, and the material can be discharged. Subsequently, the material is fermented in an open aging workshop for 15 days to naturally reduce the temperature of the pile, and gradually stop emitting stench and attracting mosquitoes and flies. The pile is 5m wide and 3m high, with a triangular cross-section. It is turned over once every 2 days, and then the fermented semi-finished fertilizer is passed through a drying device to reduce its moisture content to 20%, thus obtaining fermented organic fertilizer.

[0066] The volume ratio of the suspension of Giant Aspergillus oryzae to the suspension of Pseudomonas oryzae in the composite microbial inoculant is 1:1;

[0067] The number of viable bacteria in the suspension of Aspergillus niger and the suspension of Pseudomonas oryzae was 1.0×10 10 cfu / mL.

[0068] Compared with Example 1, in Comparative Example 2, the composite microbial agent consists of a suspension of Aspergillus niger and a suspension of Pseudomonas oryzae.

[0069] Comparative Example 3

[0070] A method for preparing a multi-functional microbial organic compound fertilizer comprises the following steps:

[0071] The fermented organic fertilizer is conveyed to a mixing and crushing machine by a collecting conveyor, the mesh number of the crushing is 80 meshes, and the composite microbial agent is sprayed at the same time, and the dosage ratio of the fermented organic fertilizer and the composite microbial agent is 1000g:6mL, and then it is packaged by an automatic packaging machine to obtain a multi-effect microbial organic compound fertilizer;

[0072] The preparation method of fermented organic fertilizer is as follows: the raw materials of sheep manure, chicken manure and crushed straw are mixed in a mass ratio of 3:1:1, and the initial organic matter content of the mixed material is adjusted to 50%, the initial water content is 50%, and the carbon-nitrogen ratio is 25:1. The Dano-type fermentation drum is used for high-temperature aerobic fermentation, the fermentation tank is filled to 0.5, and the air supply volume is 0.2m 3 / min / m 3 The fermentation tank rotates at a speed of 10r / h. During the fermentation period, the temperature inside the pile is observed by a temperature sensor every day. When the temperature at the core of the pile reaches above 50°C, the rotation speed is increased to 20r / h. After 3 days of continuous fermentation, the temperature of the pile begins to drop, the stench and fly bites are reduced, and the material can be discharged. Subsequently, the material is fermented in an open aging workshop for 15 days to naturally reduce the temperature of the pile, and gradually stop emitting stench and attracting mosquitoes and flies. The pile is 5m wide and 3m high, with a triangular cross-section. It is turned over once every 2 days, and then the fermented semi-finished fertilizer is passed through a drying device to reduce its moisture content to 20%, thus obtaining fermented organic fertilizer.

[0073] The volume ratio of the suspension of Giant Aspergillus shellfish and the suspension of Indian Pear-Shaped Spore in the composite microbial inoculant is 1:1;

[0074] The viable bacterial counts in the suspensions of Giant Aspergillus shellii and Indian Pear-shaped spores were both 1.0×10 10 cfu / mL.

[0075] Compared with Example 1, in Comparative Example 3, the composite microbial agent consists of a suspension of the giant spore fungus and a suspension of the Indian pear-shaped spore.

[0076] Comparative Example 4

[0077] A method for preparing a multi-functional microbial organic compound fertilizer comprises the following steps:

[0078] The fermented organic fertilizer is conveyed to a mixing and crushing machine by a collecting conveyor, the mesh number of the crushing is 80 meshes, and the composite microbial agent is sprayed at the same time, and the dosage ratio of the fermented organic fertilizer and the composite microbial agent is 1000g:6mL, and then it is packaged by an automatic packaging machine to obtain a multi-effect microbial organic compound fertilizer;

[0079] The preparation method of fermented organic fertilizer is as follows: the raw materials of sheep manure, chicken manure and crushed straw are mixed in a mass ratio of 3:1:1, and the initial organic matter content of the mixed material is adjusted to 50%, the initial water content is 50%, and the carbon-nitrogen ratio is 25:1. The Dano-type fermentation drum is used for high-temperature aerobic fermentation, the fermentation tank is filled to 0.5, and the air supply volume is 0.2m 3 / min / m 3 The fermentation tank rotates at a speed of 10r / h. During the fermentation period, the temperature inside the pile is observed by a temperature sensor every day. When the temperature at the core of the pile reaches above 50°C, the rotation speed is increased to 20r / h. After 3 days of continuous fermentation, the temperature of the pile begins to drop, the stench and fly bites are reduced, and the material can be discharged. Subsequently, the material is fermented in an open aging workshop for 15 days to naturally reduce the temperature of the pile, and gradually stop emitting stench and attracting mosquitoes and flies. The pile is 5m wide and 3m high, with a triangular cross-section. It is turned over once every 2 days, and then the fermented semi-finished fertilizer is passed through a drying device to reduce its moisture content to 20%, thus obtaining fermented organic fertilizer.

[0080] The volume ratio of the suspension of Giant Aspergillus oryzae, the suspension of Fusarium oryzae and the suspension of Pseudomonas oryzae in the composite microbial agent is 1:1:1;

[0081] The viable counts of the suspensions of Giant Aspergillus shellii, Falcaria oryzae, and Pseudocalcaria oryzae were all 1.0×10 10 cfu / mL.

[0082] Compared with Example 1, in Comparative Example 4, the composite microbial agent consists of a suspension of Macrocystis oryzae, a suspension of Fusarium oryzae, and a suspension of Pseudomonas oryzae.

[0083] The microbial organic compound fertilizers prepared in Example 1 and Comparative Examples 1-4 were applied to the soil under the same planting conditions. The experimental location was Huzhou City, Zhejiang Province. The experiment was divided into 5 groups, each with three test plots. The application rate was 1000kg / 667m 2 The crop is Melon Huxiang No. 1. The per-acre yield of melon and the incidence of wilt disease are counted. The test results are shown in the following table:

[0084]

[0085]

[0086] Finally, it should be noted that the above embodiments do not limit the present invention in any form. For those skilled in the art, some modifications and improvements can be made to the present invention. Therefore, any modification or improvement made without departing from the spirit of the present invention belongs to the scope of protection claimed in the present invention.

Claims

1. A multi-functional microbial organic compound fertilizer, characterized in that: The microbial organic compound fertilizer is composed of fermented organic fertilizer and compound microbial agent, wherein the compound microbial agent is composed of suspension of Giant Aspergillus oryzae, suspension of Sickle bottle of rice, suspension of Pseudomonas oryzae, and suspension of Indian Piriformis.

2. The multi-functional microbial organic compound fertilizer according to claim 1, characterized in that: The dosage ratio of the fermented organic fertilizer and the composite microbial agent is 1000g:4-8mL.

3. The multi-functional microbial organic compound fertilizer according to claim 1, characterized in that: The preparation method of the fermented organic fertilizer is as follows: sheep manure, chicken manure and straw powder are mixed and fermented to obtain the organic fertilizer.

4. The multi-functional microbial organic compound fertilizer according to claim 3, characterized in that: The mass ratio of the sheep manure, chicken manure and straw powder is 2-4:1-2:1-2.

5. The multi-functional microbial organic compound fertilizer according to claim 1, characterized in that: The volume ratio of the suspension of Giant Aspergillus shellfish, the suspension of Sickle bottle mold of rice, the suspension of Pseudomonas oryzae and the suspension of Indian Pear-shaped spore in the composite microbial agent is 1-2:1-2:1-2:1-2.

6. The multi-functional microbial organic compound fertilizer according to claim 1, characterized in that: The number of viable bacteria in the suspension of Giant Aspergillus shell, the suspension of Falcaria oryzae, the suspension of Pseudomonas oryzae and the suspension of Piriformis indica was not less than 1.0×10 9 cfu / mL.

7. A method for preparing the multi-functional microbial organic compound fertilizer according to any one of claims 1 to 6, characterized in that: The method comprises the following steps: conveying the fermented organic fertilizer to a mixing and crushing machine through a collecting conveyor, spraying a composite microbial agent, and then packaging the fertilizer through an automatic packaging machine to obtain a multi-functional microbial organic composite fertilizer.

8. The preparation method according to claim 7, characterized in that: The mesh size of the mixing mill is 60-80 mesh.

9. Application of the multi-functional microbial organic compound fertilizer according to any one of claims 1 to 6 in agricultural production.

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