An optical imaging and electrophysiological recording window fixation device

Abstract

The application discloses an optical imaging and electrophysiological recording window fixing device, which comprises a window base, a window pressing flange ring, a fixed hollow screw rod and a top cover. The optical imaging and electrophysiological recording window fixing device does not need to repeatedly cut the scalp and perform a window opening operation, and only needs to be installed once, and then multiple experiments can be repeatedly performed, so that the repeated damage to experimental animals is reduced; normal intracranial pressure can be maintained, and the window can be airtight and infection-proof. The window base is designed to be firm, and can be fixed to the surface of the skull through dental cement or bone cement. The window pressing flange ring can be replaced and selected, and is suitable for different animals, different sizes and different depth imaging windows. During the interval of experiments, the top cover is used for sealing, and the window can be effectively sealed, tissue hyperplasia or infection is reduced, the operation is simple, and the window is effectively sealed. The device is compatible with an electrophysiological positioning device, and electrode implantation is facilitated.

Description

Technical Field

[0001] The invention belongs to the field of biotechnology, and in particular relates to an optical imaging and electrophysiological recording window fixing device. Background Art

[0002] Endogenous optical imaging uses a highly sensitive CCD camera to image optical signals from the cerebral cortex. By reflecting light at specific wavelengths, it detects changes in local oxygen supply caused by neuronal activity, thereby revealing brain activity. Optical imaging typically reflects signals from the surface of the cerebral cortex. Researchers use this technology to obtain cortical functional maps of brain regions of interest. Furthermore, it can be combined with electrophysiological techniques to detect neuronal activity at specific functional locations at different cortical depths, thereby revealing more systematically the functions of brain regions.

[0003] In many studies, we need to repeatedly image and perform electrophysiological recordings on the same region of the brain in experimental animals. This requires a reliable and stable device that can meet experimental requirements while also being able to maintain continuous maintenance to prevent infection and tissue proliferation, allowing for repeated experiments.

[0004] In previous repeated experimental protocols, researchers used flange rings to connect glass slides, pressed them against the brain surface, and temporarily fixed them to the skull with glue using several small wooden strips for imaging experiments. At the end of the experiment, they used artificial meninges to press the window, put it back into the skull, sealed the window with bone cement, and sutured the wound. Secondary or multiple experiments required scalp incision and window surgery at the same location. This protocol inevitably caused repeated surgical damage to the animals. At the same time, the surgical operation increased the duration of the anesthesia experiment, which was not conducive to experimental data collection and was also not conducive to animal recovery.

[0005] For example, the invention application with publication number CN113855303A discloses a brain drug administration observation cranial window and an experimental animal fixed observation device. The brain drug administration observation cranial window includes a main body and a light-transmitting plate. The main body is provided with a protrusion, and the end face of the protrusion is provided with a light-transmitting hole and a first perforation. The circumferential surface of the protrusion is provided with a second perforation. The first perforation and the second perforation are used to cooperate with the drug administration tube of the drug administration pump, and the light-transmitting plate is matched with the protrusion to cover the light-transmitting hole.

[0006] For example, the utility model with authorization announcement number CN219166501U discloses a skull tissue sealing device and a craniotomy imaging device. The skull tissue sealing device includes an imaging glass slide and a sealing ring. The optical axis of the imaging glass slide coincides with the central axis of the sealing ring, and one end of the sealing ring is sealed with the imaging glass slide to form a container. A cavity for accommodating a hydrogel is formed inside the container, and an opening is formed at the other end of the sealing ring. The outer surface of the sealing ring is provided with a glue layer for sealing and bonding with the skull. Summary of the Invention

[0007] To address these issues, we designed an imaging and electrophysiology window fixture that can be permanently fixed to the animal's head and easily removed for cleaning. Experimental testing has shown that this device meets the window requirements for imaging and electrophysiology experiments, while also facilitating long-term maintenance and allowing for repeated experiments.

[0008] An optical imaging and electrophysiological recording window fixing device, comprising:

[0009] The window base is used to fit on the surface of the skull, the window base has a mounting slot, the bottom of the mounting slot has a step structure, and the portion of the mounting slot above the step structure has an internal thread;

[0010] The window-pressing flange ring includes a pressure ring and a retaining ring extending downward from the inner circle of the bottom surface of the pressure ring. When in use, the lower surface opening of the retaining ring is equipped with a glass slide and extends into the target window of the skull to be installed. The pressure ring overlaps the step structure. The glass slide used in the maintenance stage has no hole, while the glass slide used during electrophysiological testing has a lower electrode hole.

[0011] The fixed hollow screw is a ring with an external thread. When in use, the fixed hollow screw cooperates with the internal thread of the window base to be installed in the installation slot and presses and fixes the window pressing flange ring;

[0012] The top cover is fixedly mounted on the window base during the maintenance phase and is used to cover the mounting slot.

[0013] Preferably, the outer periphery of the top surface of the window base has a plurality of threaded fixing holes, and the top cover is correspondingly provided with a first mounting hole, and the top cover and the window base are fixed by bolts passing through the first mounting holes and cooperating with the threaded fixing holes.

[0014] More preferably, the optical imaging and electrophysiological recording window fixing device further comprises a flange ring fixing part, the flange ring fixing part having a second mounting hole corresponding to the position of the threaded fixing hole on the window base, the structure of the flange ring fixing part avoiding the mounting slot hole, and the flange ring fixing part having a fixing arm extending toward the mounting slot hole position, the fixing arm being provided with a threaded fixing through hole, and the window pressing flange ring being fixed by a fixing bolt matched with the threaded fixing through hole pressing against the top surface of the pressure ring of the window pressing flange ring;

[0015] During use, the fixing hollow screw is used to compress and fix the window pressing flange ring during the maintenance phase, and the flange ring fixing piece is used to compress and fix the window pressing flange ring during electrophysiological testing.

[0016] Further preferably, the optical imaging and electrophysiological recording window fixing device further comprises an electrophysiological positioning device connector, the electrophysiological positioning device connector having a fixing area at the bottom and a supporting table at the top, the fixing area having a third mounting hole corresponding to the position of the remaining threaded fixing hole on the window base, and the structure of the electrophysiological positioning device connector avoids the mounting slot hole.

[0017] During use, during electrophysiological testing, the electrophysiological positioning device connector is fixed to the window base by a bolt passing through the third mounting hole, the electrode mounting arm of the electrophysiological recording device is supported on the support table, and the electrode extends into the mounting slot and passes through the lower electrode hole on the glass slide.

[0018] Further preferably, the window base is provided with four threaded fixing holes located at four corners, the flange ring fixing piece is provided with two second mounting holes, and the fixing area of ​​the electrophysiological positioning device connector is provided with two third mounting holes.

[0019] Preferably, during use, the window base is fitted on the skull surface using dental cement or bone cement, and the periphery of the window base is provided with grooves for increasing friction, wherein the grooves are arranged vertically at intervals or crisscrossed.

[0020] Preferably, the bottom surface of the window base has a lower edge extending outwardly along the installation slot, and when in use, the lower edge extends into the target window of the skull to be installed.

[0021] Preferably, a wrench action groove is provided on the top surface of the fixed hollow screw.

[0022] Preferably, a convex ring extending into the mounting slot is convexly provided on the bottom surface of the top cover, and the outer diameter of the convex ring matches the mounting slot.

[0023] The present invention further provides an electrophysiological recording method, wherein the optical imaging and electrophysiological recording window fixing device is installed on the surface window of the skull of an experimental animal.

[0024] During electrophysiological recording, the top cover is opened for testing; after the testing is completed, the top cover is closed.

[0025] When the optical imaging and electrophysiological recording window fixing device of the present invention is used, the window installation steps are as follows:

[0026] (1) The dura mater is exposed by opening a target window on the surface of the skull of the experimental animal, wherein the size of the window matches the size of the retaining ring at the bottom of the window pressing flange ring.

[0027] (2) Grind the surface near the window of the skull flat, fit the window base to the skull surface, and align the mounting slot with the window position. To ensure alignment, when installing the window base, place the window pressing flange ring into the window base, and extend the retaining ring at the bottom of the window pressing flange ring into the window to play a positioning role. Then, use dental cement or bone cement to apply to the periphery and bottom of the window base, wait for it to solidify, and then fix the window base to the skull.

[0028] (3) Clean the window, peel off the dura mater to expose the pia mater, and measure the depth with a tape measure. Then, install a window-pressing flange ring with a glass slide (without holes) bonded to the bottom and an overall thickness that matches the window depth into the window base. To improve the sealing effect, a silicone sealing ring can be placed between the bottom surface of the window-pressing flange ring and the stepped structure of the window base.

[0029] (4) Tighten the fixed hollow screw into the window base to tighten the window flange ring below. Observe the surface and periphery of the glass slide. If there is no liquid seepage, or try to drop physiological saline on the surface and the depth does not change, it means that the seal is good.

[0030] (5) After the test is completed, add a few drops of antibiotic injection solution to the sealed surgical window, then install the top cover to the top surface of the window base, and seal the surrounding gaps again with bone wax to complete the window installation steps.

[0031] When conducting optical imaging inspection, you only need to open the top cover to conduct observation and inspection.

[0032] When conducting electrophysiological recording tests, you need to open the top cover first, then unscrew the fixed hollow screw, replace the window-pressing flange ring with the same model but with a lower electrode hole left on the glass slide, and install the electrode for testing.

[0033] The optical imaging and electrophysiological recording window fixing device of the present invention does not require repeated scalp cutting and window opening surgery. It only needs to be installed once, and multiple experiments can be repeated subsequently, reducing repeated damage to experimental animals; it can maintain normal intracranial pressure and seal the window to prevent infection.

[0034] The window base is designed to be securely fixed to the skull surface using dental cement or bone cement. The window flange is interchangeable to accommodate different animals, imaging windows of varying sizes, and depths. During breaks in experiments, the top cover provides a seal, ensuring a secure window seal and minimizing tissue growth and infection. Compatible with electrophysiological positioning devices, the device facilitates electrode placement. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 This is a schematic diagram of the assembly structure of the optical imaging and electrophysiological recording window fixing device of the present invention during the maintenance stage.

[0036] Figure 2 This is a schematic diagram of the assembly structure of the optical imaging and electrophysiological recording window fixing device of the present invention during the maintenance stage from another perspective.

[0037] Figure 3 This is a cross-sectional view of the assembly structure of the optical imaging and electrophysiological recording window fixing device of the present invention during the maintenance stage.

[0038] Figure 4 This is a schematic diagram of the exploded structure of the assembly structure of the optical imaging and electrophysiological recording window fixing device of the present invention during the maintenance stage.

[0039] Figure 5 Schematic diagram of the assembly structure for electrophysiological recording testing.

[0040] Figure 6 It is a structural diagram of the flange ring fixing.

[0041] Figure 7 This is a schematic diagram of the structure of the connector of the electrophysiological positioning device.

[0042] Figure 8 Schematic diagram of the assembly structure during optical imaging detection.

[0043] Figure 9 This is a cross-sectional view of the assembly structure during optical imaging inspection.

[0044] Figure 10 Schematic diagram of the structure of a hollow screw fastener.

[0045] Reference numerals:

[0046] Window base 1, step structure 11, threaded fixing hole 12, groove 13, bottom edge 14,

[0047] Window pressure flange ring 2, pressure ring 21, retaining ring 22,

[0048] Fixed hollow screw 3, wrench action groove 31,

[0049] Top cover 4, first mounting hole 41, convex ring 42,

[0050] Flange ring fixing member 5, second mounting hole 51, fixing arm 52, threaded fixing through hole 53,

[0051] Electrophysiological positioning device connector 6, fixing area 61, support table 62, third mounting hole 63,

[0052] Tightening wrench 7. DETAILED DESCRIPTION

[0053] like Figures 1 to 4As shown, an optical imaging and electrophysiological recording window fixing device is an assembly diagram structure in the maintenance stage, including a window base 1, a window pressing flange ring 2, a fixing hollow screw 3 and a top cover 4.

[0054] The window base 1 is used to fit on the surface of the skull. The window base 1 is an annular structure as a whole, with a mounting slot in the middle. The bottom inner side of the mounting slot has a step structure 11, and the inner side of the mounting slot above the step structure 11 has an internal thread.

[0055] During use, the window base 1 is attached to the skull surface using dental cement or bone cement. The outer periphery of the window base 1 is provided with grooves 13 to increase friction. Multiple grooves 13 are arranged vertically and spaced apart. Dental cement or bone cement can be embedded in grooves 13, making the installation and fixation of the window base 1 more secure.

[0056] In one embodiment, Figure 3 In the cross-sectional view shown, the bottom surface of the window base 1 is a plane. In another embodiment, as shown in FIG. Figure 9 As shown, the bottom surface of the window base 1 has a lower edge 14 extending outward along the installation slot. When in use, the lower edge 14 extends into the target window of the skull to be installed.

[0057] The window-pressing flange ring 2 includes a pressure ring 21 and a retaining ring 22 extending downward from the inner circle of the bottom surface of the pressure ring 21. During use, a glass slide (not shown) is installed on the lower surface of the retaining ring 22 and inserted into the target window of the skull to be installed. The pressure ring 21 overlaps the step structure 11. The glass slide used during the maintenance phase has no holes, while the glass slide used during electrophysiological testing has a hole for the lower electrode.

[0058] The window pressing flange ring 2 needs to take into account windows of different sizes and depths on the skull, so the window pressing flange ring 2 can be designed with different diameters and different heights of the retaining ring 22. When in use, the window pressing flange ring 2 is fixed to the glass slide of the corresponding model with UV-curing glue. The glass slide is round and its diameter is equal to the outer diameter of the retaining ring 22. During the experiment, the selection is made according to the specific situation. The depth selection of the window pressing flange ring 2 ensures that a matching depth can be selected during the experiment, that is, the cortical surface fluctuation caused by the retaining ring 22 of the window pressing flange ring 2 being inserted too shallowly will not affect the optical imaging, nor will the local cortical blood supply be insufficient due to the retaining ring 22 of the window pressing flange ring 2 being inserted too deeply.

[0059] The fixed hollow screw 3 is a ring with external threads. When in use, the fixed hollow screw 3 is matched with the internal threads of the window base 1 and installed in the installation slot, and presses and fixes the window pressing flange ring 2. The top surface of the fixed hollow screw 3 is provided with a wrench action groove 31. Figure 10The figure shows a tightening wrench 7 for installing and removing the fixed hollow screw 3. The bottom of the tightening wrench 7 is inserted into two opposing wrench action grooves 31, and then rotated to fix the fixed hollow screw 3 to the window base 1 or remove it from the window base 1. In the structure shown in the figure, the top surface of the fixed hollow screw 3 is provided with four wrench action grooves 31. The four wrench action grooves 31 are evenly distributed in a circle, with two opposing wrench action grooves 31 forming a group.

[0060] The top cover 4 is a cover-like structure, which is fixed to the window base 1 during the maintenance phase and is used to cover the installation slots.

[0061] The top surface of the window base 1 has a plurality of threaded fixing holes 12 on its periphery, and the top cover 4 is correspondingly provided with a first mounting hole 41. The top cover 4 and the window base 1 are fixed by bolts (bolts not shown in the figure) that pass through the first mounting holes 41 and cooperate with the threaded fixing holes 12.

[0062] The bottom surface of the top cover 4 is provided with a convex ring 42 which extends into the mounting slot, and the outer diameter of the convex ring 42 matches the mounting slot. The provision of the convex ring 42 can improve the sealing performance.

[0063] like Figures 5-6 As shown, the optical imaging and electrophysiological recording window fixing device of the present invention further includes a flange ring fixing member 5 having a second mounting hole 51 corresponding to a portion of the threaded fixing hole 12 on the window base 1. A fixing bolt (not shown) passing through the second mounting hole 51 engages with the threaded fixing hole 12 to secure the flange ring fixing member 5 to the window base 1. The flange ring fixing member 5 is structured to avoid the mounting slot of the window base 1 and is generally a semicircular ring structure.

[0064] The flange ring fixing part 5 has a fixing arm 52 extending toward the mounting slot position, and a threaded fixing through hole 53 is provided on the fixing arm 52. The window pressure flange ring 2 is fixed by a fixing bolt (not shown in the figure) that cooperates with the threaded fixing through hole 53 and presses against the top surface of the pressure ring 21 of the window pressure flange ring 2.

[0065] During use, the fixed hollow screw 3 is used in conjunction with the fixing bolt to compress and fix the window-pressing flange ring 2 during maintenance, while the flange ring fixing piece 5 is used to compress and fix the window-pressing flange ring 2 during electrophysiological testing. During electrophysiological testing, we need to select multiple recording sites based on the experimental purpose in combination with the functional map obtained by optical imaging. Therefore, the position of the electrode needs to be adjusted during a single experiment. If the fixed hollow screw 3 is still used to compress and fix the window-pressing flange ring 2, each adjustment requires first loosening the fixed hollow screw 3 and then tightening the fixed hollow screw 3 after the adjustment is completed, which is very troublesome to operate and use.

[0066] Therefore, the present invention uses a flange ring fixing part 5 in conjunction with a fixing bolt to temporarily fix the window pressure flange ring 2 during electrophysiological testing. When adjusting, it is only necessary to loosen the fixing bolt to adjust the angle of the window pressure flange ring 2, thereby adjusting the electrode position. After adjustment, re-tighten the fixing bolt to fix the window pressure flange ring 2. The entire adjustment process is relatively simple and convenient.

[0067] like Figure 5 and Figure 7 As shown, the optical imaging and electrophysiological recording window fixing device of the present invention also includes an electrophysiological positioning device connector 6. The electrophysiological positioning device connector 6 has a fixing area 61 at the bottom and a support platform 62 at the top. The fixing area 61 has a third mounting hole 63 corresponding to the position of the remaining threaded fixing hole 12 on the window base 1. The electrophysiological positioning device connector 6 is fixed to the window base 1 by a fixing bolt (not shown) passing through the third mounting hole 63 and cooperating with the threaded fixing hole 12. The structure of the electrophysiological positioning device connector 6 avoids the installation slot.

[0068] During use, during electrophysiological testing, the electrophysiological positioning device connector 6 is fixed to the window base 1 by a bolt passing through the third mounting hole 63, and the hydraulic micro-push device of the electrophysiological recording electrode is fixed to the support table 62 by a screw. The electrode extends into the mounting slot and passes through the lower electrode hole on the glass slide. This fixed setting can effectively ensure that the electrode implantation trajectory is perpendicular to the cortical mark surface.

[0069] In a preferred embodiment, Figure 7 As shown, the window base 1 is provided with four threaded fixing holes 12 at the four corners, the flange ring fixing member 5 is provided with two second mounting holes 51, and the fixing area 61 of the electrophysiological positioning device connector 6 is provided with two third mounting holes 63. The flange ring fixing member 5 and the electrophysiological positioning device connector 6 are respectively fixed to the window base 1 using fixing bolts.

[0070] When the optical imaging and electrophysiological recording window fixing device of the present invention is used, the window installation steps are as follows:

[0071] (1) The dura mater is exposed by opening a target window on the surface of the skull of the experimental animal, and the size of the window matches the size of the retaining ring 22 at the bottom of the window pressing flange ring 2.

[0072] (2) Smooth the surface near the window of the skull and fit the window base 1 to the skull surface, aligning the mounting slot with the window position. To ensure alignment, when installing the window base 1, place the window pressing flange ring 2 into the window base 1, and extend the retaining ring 22 at the bottom of the window pressing flange ring 2 into the window to play a positioning role. Then, apply dental cement or bone cement to the periphery and bottom of the window base 1, wait for it to solidify, and then fix the window base 1 to the skull.

[0073] (3) Clean the window, peel off the dura mater to expose the cortical pia mater, measure the depth with a tape measure, and then install the window-pressing flange ring 2, which has a glass slide (without holes) bonded to the bottom and has an overall thickness that matches the window depth, into the window base 1. To improve the sealing effect, a silicone sealing ring can be placed between the bottom surface of the pressure ring 21 of the window-pressing flange ring 2 and the step structure 11 of the window base 1.

[0074] (4) Tighten the fixed hollow screw 3 into the window base 1 to tighten the window pressing flange ring 2 below. Observe the surface and periphery of the glass slide. If there is no liquid seepage, or try to drop physiological saline on the surface and the depth does not change, it means that the seal is good.

[0075] (5) After the test is completed, the top cover 4 is installed on the top surface of the window base 1, and the gap is sealed again with bone wax to complete the window installation steps.

[0076] During optical imaging testing, simply open the top cover 4 for observation and testing. After the experiment, before installing the top cover 4, add a small amount of antibiotic injection to the mounting slot to prevent infection. Then, install the top cover 4 and further seal the joint between the top cover 4 and the window base 1 with bone wax to facilitate subsequent testing.

[0077] When conducting electrophysiological recording tests, it is necessary to first open the top cover 4, then unscrew the fixed hollow screw 3, and replace the window-pressing flange ring 2 with the same model but with a lower electrode hole on the glass slide. Then, use the fixing bolts 5 and the electrophysiological positioning device connector 6 to fix them to the window base 1 respectively. Then, use the fixing bolts that match the threaded fixing through-holes 53 to press against the top surface of the pressure ring 21 of the window-pressing flange ring 2 to fix the window-pressing flange ring 2. The electrode mounting arm of the electrophysiological recording device is supported on the support table 62, and the electrode extends into the mounting slot and passes through the lower electrode hole on the glass slide. When the electrode position needs to be adjusted, loosen the fixing bolts used to tighten the window-pressing flange ring 2, then adjust the angle of the window-pressing flange ring 2 to adjust the electrode position. After adjustment, re-tighten the fixing bolts to fix the window-pressing flange ring 2. After the test is completed, re-disassemble the flange ring fixing 5 and the electrophysiological positioning device connector 6, and then use the fixed hollow screw 3 to re-tighten the window-pressing flange ring 2. Before installing the top cover 4, a small amount of antibiotic injection was added to the installation slot to prevent infection. The top cover 4 was then installed and bone wax was used to further seal the joint between the top cover 4 and the window base 1 so that subsequent experiments could be carried out again.

Claims

1. An optical imaging and electrophysiological recording window fixing device, characterized in that: include: The window base is used to fit on the surface of the skull, the window base has a mounting slot, the bottom of the mounting slot has a step structure, and the portion of the mounting slot above the step structure has an internal thread; A window-pressing flange ring comprises a pressure ring and a retaining ring extending downward from the inner ring of the bottom surface of the pressure ring. When in use, a glass slide fixed with UV-curing glue is installed on the lower surface of the retaining ring. The retaining ring with the glass slide extends into the target window of the skull to be installed. The pressure ring overlaps the step structure. The glass slide used in the maintenance stage has no holes, while the glass slide used during electrophysiological testing has a hole for the lower electrode. The fixed hollow screw is a ring with an external thread. When in use, the fixed hollow screw cooperates with the internal thread of the window base to be installed in the installation slot and presses and fixes the window pressing flange ring; A top cover, which is fixed to the window base during maintenance and is used to cover the installation slot; The top surface of the window base has a plurality of threaded fixing holes on its outer periphery, and the top cover is correspondingly provided with a first mounting hole, and the top cover and the window base are fixed by bolts passing through the first mounting holes and cooperating with the threaded fixing holes; The optical imaging and electrophysiological recording window fixing device also includes a flange ring fixing part, the flange ring fixing part has a second mounting hole corresponding to the position of the threaded fixing hole on the window base, the structure of the flange ring fixing part avoids the mounting slot hole, and the flange ring fixing part has a fixing arm extending toward the mounting slot hole position, the fixing arm is provided with a threaded fixing through hole, and the window pressing flange ring is fixed by a fixing bolt that cooperates with the threaded fixing through hole against the top surface of the pressure ring of the window pressing flange ring; During use, the fixing hollow screw is used to compress and fix the window pressing flange ring during the maintenance phase, and the flange ring fixing piece is used to compress and fix the window pressing flange ring during electrophysiological testing.

2. The optical imaging and electrophysiological recording window fixing device according to claim 1, characterized in that: It also includes an electrophysiological positioning device connector, which has a fixing area at the bottom and a supporting table at the top. The fixing area has a third mounting hole corresponding to the position of the remaining threaded fixing holes on the window base. The structure of the electrophysiological positioning device connector avoids the mounting slot hole. During use, during electrophysiological testing, the electrophysiological positioning device connector is fixed to the window base by a bolt passing through the third mounting hole, the electrode propulsion device mounting arm of the electrophysiological recording device is supported on the support table, and the electrode extends into the mounting slot and passes through the lower electrode hole on the glass slide.

3. The optical imaging and electrophysiological recording window fixing device according to claim 2, characterized in that: The window base is provided with four threaded fixing holes located at four corners, the flange ring fixing piece is provided with two second mounting holes, and the fixing area of ​​the electrophysiological positioning device connecting piece is provided with two third mounting holes.

4. The optical imaging and electrophysiological recording window fixing device according to claim 1, characterized in that: When in use, the window base is fitted on the surface of the skull using dental cement or bone cement, and a groove is provided on the periphery of the window base for increasing friction.

5. The optical imaging and electrophysiological recording window fixing device according to claim 1, characterized in that: The bottom surface of the window base has a lower edge extending outward along the installation slot. When in use, a circular skull window is opened with an area slightly larger than the lower edge of the base, and the lower edge is extended into the target window of the skull to be installed.

6. The optical imaging and electrophysiological recording window fixing device according to claim 1, characterized in that: The top surface of the fixed hollow screw is provided with a wrench action groove.

7. The optical imaging and electrophysiological recording window fixing device according to claim 1, characterized in that: The bottom surface of the top cover is convexly provided with a convex ring extending into the installation slot hole, and the outer diameter of the convex ring matches the installation slot hole.

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