Sorghum bicolor bialistie trait gene SbDG2 and its application in regulating the formation of bicolor sorghum

By cloning the SbDG2 gene of the sorghum double-grain trait and editing the gene using the CRISPR/Cas9 system, the problem of unclear genetic basis of the sorghum double-grain trait was solved, and the regulation of the double-grain trait in sorghum breeding was realized, which improved the number of grains per ear and the yield.

CN119351458BActive Publication Date: 2026-07-03INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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Patent Information

Application Number
CN202411714908.9
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-11-27
Publication Date
2026-07-03
Estimated Expiration
2044-11-27

AI Technical Summary

Technical Problem

The genetic basis and molecular mechanism of the double-grain trait in sorghum are unclear in the existing technology, making it difficult to effectively promote the formation of double seeds on spikelets in plants.

Method used

The SbDG2 gene, which controls the double-seed trait in sorghum, was cloned. By regulating the expression or activity of the SbDG2 protein, gene editing was performed using the CRISPR/Cas9 system to promote abnormal development of spikelet florets in sorghum to form double seeds.

Benefits of technology

This study improved the number of grains per sorghum ear and the yield, realizing the regulation of two grain traits in sorghum breeding, which has important theoretical and applied value.

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Abstract

This invention discloses the sorghum double-grain trait gene SbDG2 and its application in regulating double-grain sorghum formation. This invention provides the application of any one of the following substances (A1)-A3) in any one of the following (E1)-E4): E1) regulating the number of grains per spike; E2) regulating plant yield; E3) regulating the development of spikelet florets; E4) regulating the formation of double seeds per spikelet; A1) protein SBDG2; A2) nucleic acid molecule encoding protein SBDG2; A3) recombinant vector, expression cassette, or recombinant bacteria containing a nucleic acid molecule encoding protein SBDG2. This invention clones a gene SbDG2 that controls the double-grain trait in sorghum, and genetic breeding using this gene can effectively increase the number of grains per spike and the yield of sorghum.
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