Stem cell culture device

Abstract

The invention discloses a stem cell culture device, and relates to the technical field of stem cell culture, the stem cell culture device comprises a culture dish fixing mechanism and a clamping support mounted in the culture dish fixing mechanism, a rotary culture mechanism is arranged on the outer side of the culture dish fixing mechanism, and an illumination lamp is arranged in the rotary culture mechanism; the culture dish fixing mechanism comprises a fixing disc, a plurality of mounting holes are formed in the fixing disc, clamping gear rings are fixedly mounted at the center positions of the top and the bottom of the fixing disc, clamping gears are connected to the interiors of the clamping gear rings in a clamping manner, first connecting rods are fixedly mounted at the tops of the clamping gears, and the first connecting rods are fixedly connected to the bottoms of the first connecting rods. A movable plate is lifted upwards and a movable spring is extruded, so that the movable plate drives a first connecting rod and a clamping gear to move, when the clamping gear is separated from a clamping gear ring, a fixed disc can be taken out from the interior of the incubator, all culture dishes on the fixed disc can be taken out, and meanwhile, the phenomenon that the culture dishes are disordered cannot be caused.

Description

Technical Field

[0001] The present invention relates to the technical field of stem cell culture, in particular to a stem cell culture device. Background Art

[0002] Stem cells are a type of multipotent cells with the ability to self-replicate. Under certain conditions, they can differentiate into a variety of functional cells. According to the developmental stage of the stem cells, they are divided into embryonic stem cells and adult stem cells. Stem cells are a type of undifferentiated, immature cell that has the potential to regenerate various tissues, organs and the human body. The medical community calls them "universal cells", so the cultivation of stem cells is particularly important.

[0003] Announcement No. CN215162809U discloses a stem cell culture device, including a box body, bases are respectively arranged on the walls around the bottom of the box body, a sealed door is arranged on the wall body on one side of the front end of the box body, an outer door is also arranged on the wall body on the side close to the sealed door at the front end of the box body, a drainage hole is also opened in the wall body on one side below the front end of the box body, fixed frames are respectively arranged on the front and rear end walls on both sides of the box body, a shelf is arranged between the two fixed frames, a clamping mechanism is arranged on the rear end wall body on the top of the shelf, the clamping mechanism includes a fixing rod, a connecting rod and a pressing plate, a gas filter is also arranged on the wall body on the top side of the box body, a water injection hole is also arranged in the wall body on one side above the front end of the box body, and a controller is also arranged on the wall body in the middle position above the front end of the box body, and the culture dishes or culture bottles of different heights can be clamped and fixed by setting the detachable clamping mechanism, and when the culture dishes or culture bottles are placed in stacks, other culture dishes or culture dishes may fall due to contact, but the patent still has the following problems in actual use:

[0004] Although the stem cell culture device can clamp and fix culture dishes or culture bottles of different heights by means of a detachable clamping mechanism, other culture dishes or culture bottles may fall due to contact when placed on stacked culture dishes or culture bottles. However, due to the different diameters of the culture dishes, the curvature of the outer side of the culture dishes is different, and the curvature plate cannot be adjusted according to the culture dishes of different diameters, resulting in the curvature plate being unable to fit the outer edge of the culture dish, thereby affecting the stability of the culture dish clamping.

[0005] Therefore, a stem cell culture device is proposed to solve the above problems. Summary of the invention

[0006] The object of the present invention is to provide a stem cell culture device to solve the problem that although the stem cell culture device proposed in the above background technology can clamp and fix culture dishes or culture bottles of different heights through the setting of a detachable clamping mechanism, when placing stacked culture dishes or culture bottles, other culture dishes or culture dishes may fall due to contact. However, due to the different diameters of the culture dishes, the curvature of the outer side of the culture dishes is different, and the curvature plate cannot be adjusted according to the culture dishes of different diameters, resulting in the curvature plate being unable to fit the outer edge of the culture dish, thereby affecting the clamping stability of the culture dish.

[0007] To achieve the above-mentioned object, the present invention provides the following technical solutions: a stem cell culture device, comprising a culture dish fixing mechanism, and a clamping bracket installed inside the culture dish fixing mechanism;

[0008] A rotating culture mechanism is arranged outside the culture dish fixing mechanism, and an illumination lamp is arranged inside the rotating culture mechanism;

[0009] Also includes:

[0010] The culture dish fixing mechanism comprises a fixing plate, a plurality of mounting holes are opened inside the fixing plate, and a clamping gear ring is fixedly installed at the center position of the top and bottom of the fixing plate;

[0011] Wherein, a locking gear is locked inside the locking gear ring, and a first connecting rod is fixedly installed on the top of the locking gear;

[0012] Wherein, a moving plate is fixedly installed on the top of the first connecting rod, and moving springs are fixedly installed around the top of the moving plate.

[0013] Preferably, a fixing plate is fixedly installed on the top of the movable spring, a second connecting rod is fixedly installed at the top center position of the fixing plate, the clamping bracket is fixedly installed inside the mounting hole, a clamping sliding rod is fixedly installed around the inside of the clamping bracket, and a clamping sliding sleeve is slidably connected to the outer side of the clamping sliding rod.

[0014] Preferably, a clamping limit plate is fixedly installed on the top of the clamping sliding sleeve, a torsion spring is symmetrically arranged on the top of the clamping limit plate, a clamping rotating shaft is arranged inside the torsion spring, the clamping rotating shaft is rotatably connected to the clamping limit plate, a clamping rotating plate is fixedly installed on the outer side of the clamping rotating shaft, the top end of the torsion spring is fixedly connected to the clamping rotating plate, and the bottom end of the torsion spring is fixedly connected to the clamping limit plate.

[0015] Preferably, the bottom of the clamping sliding sleeve is rotatably connected to a clamping rotating rod, the bottom of the clamping rotating rod is rotatably connected to a clamping lifting plate, a first through hole is opened at the inner center position of the clamping lifting plate, a reset spring is fixedly installed at the top center position of the clamping bracket, a support ring is fixedly installed on the top of the reset spring, and a fixed sleeve is fixedly installed on the bottom of the support ring.

[0016] Preferably, a plurality of fixing holes are opened around the outer side of the fixing sleeve, the internal thread of the fixing sleeve is connected with an adjusting threaded rod, a first support plate is fixedly installed on the top of the adjusting threaded rod, the adjusting threaded rod is threadedly connected to the support ring, and a fixing bracket is fixedly installed at the bottom center position of the clamping bracket.

[0017] Preferably, locking grooves are provided around the interior of the fixed bracket, the bottom of the fixed bracket is rotatably connected to an adjusting gear, spiral grooves are provided around the interior of the adjusting gear, the interior of the spiral groove is slidably connected to a limiting slider, a locking sliding rod is fixedly installed on the top of the limiting slider, and the locking sliding rod is slidably connected to the fixed bracket through the locking groove.

[0018] Preferably, a locking spring is fixedly installed at one end of the locking sliding rod, and a locking tilting block is fixedly installed at the end of the locking spring. The locking tilting block is engaged with the fixed sleeve through a fixing hole, the fixed bracket and the adjusting gear are both slidably connected to the fixed sleeve, and the adjusting threaded rod is slidably connected to the clamping lifting plate through the first through hole.

[0019] Preferably, the rotating culture mechanism includes an incubator, a plurality of fixed partition plates are fixedly installed inside the incubator, the number of the fixed partition plates is three, a second through hole is opened inside the fixed partition plates, the fixed partition plates are fixedly connected to the fixed plate through the second through hole, a plurality of rotating box doors are rotatably connected to the outside of the incubator, the number of the rotating box doors is three, a box door handle is fixedly installed on the outside of the rotating box door, and a rotating motor is fixedly installed on the inner side of the bottom of the incubator.

[0020] Preferably, the output end of the rotating motor is fixedly connected with a rotating connecting rod, and the rotating connecting rod is rotatably connected to the fixed partition plate, the illumination lamp is fixedly installed on the bottom of the fixed partition plate near the outer side of the rotating connecting rod, a rotating disk is fixedly installed on the top of the rotating connecting rod, a rotating main gear is fixedly installed on the top of the rotating disk, the top of the rotating main gear is rotatably connected with a rotating tripod, the outer side of the rotating main gear is meshingly connected with a rotating slave gear, the outer side of the rotating slave gear is meshingly connected with a meshing gear ring, the meshing gear ring is fixedly installed on the top inner side of the incubator, a second support plate is fixedly installed on the bottom of the meshing gear ring, the rotating slave gear is rotatably connected to the rotating tripod, and the rotating slave gear is fixedly connected to the second connecting rod.

[0021] Compared with the prior art, the beneficial effects of the present invention are as follows: the stem cell culture device can drive the first connecting rod and the engaging gear to move by pulling the moving plate upward and squeezing the moving spring. When the engaging gear is separated from the engaging gear ring, the fixed plate can be taken out from the inside of the culture box, which can not only take out all the culture dishes on the fixed plate, but also prevent the culture dishes from being in a mess. The rotating motor is started to drive the rotating connecting rod and the rotating plate to rotate, so that the rotating plate drives the rotating main gear to rotate. By utilizing the meshing connection characteristics of the rotating main gear, the rotating slave gear and the meshing gear ring, not only the revolution of the rotating tripod and the rotating slave gear can be realized, but also the rotation of the rotating slave gear can be realized. The specific contents are as follows:

[0022] 1. By setting the culture dish fixing mechanism, not only can the moving plate be lifted up and the moving spring be squeezed to make the moving plate drive the first connecting rod and the engaging gear to move, but when the engaging gear is separated from the engaging gear ring, the fixed plate can be taken out from the inside of the incubator, and not only all the culture dishes on the fixed plate can be taken out, but also the culture dishes will not be in disorder, which is convenient for conducting experiments on stem cells in the culture dishes. By placing the culture dish on the first supporting plate and pressing the culture dish downward, the first supporting plate squeezes the reset spring and drives the supporting ring and the fixed sleeve to move downward, so that the fixed sleeve drives the clamping lifting plate to move downward, so that the clamping rotating rod rotates and drives the clamping sliding sleeve to slide relatively on the outside of the clamping sliding rod, and the clamping limiting plate on the top of the clamping sliding sleeve is used to clamp and fix the culture dish, and the torsion spring can be used to realize the rotation of the clamping rotating plate, so as to The clamping rotating plate is made to fit culture dishes of different sizes, and culture dishes of different diameters can be clamped and fixed, thereby improving the stability of the culture dishes. When the fixed sleeve is lowered, the fixing hole and the locking inclined block can be used to engage with each other to fix the first support plate and the clamping rotating plate, thereby realizing stable clamping of the culture dishes. When other culture dishes are taken out, the remaining culture dishes will not slide off. When the culture dishes need to be taken out, the limiting slider drives the locking sliding rod to slide inside the fixed bracket by rotating the adjusting gear and under the limiting action of the spiral slide groove. When the locking inclined block is separated from the fixing hole outside the fixed sleeve, the first support plate drives the fixed sleeve and the clamping lifting plate to rise under the elastic force of the reset spring, and the clamping rotating rod drives the clamping sliding sleeve and the clamping rotating plate to expand, so that a single culture dish can be taken out from the inside of the fixed plate.

[0023] 2. By setting up a rotating culture mechanism, not only can the incubator be divided by a fixed partition plate, but also the incubator can be divided into three independent spaces, and the culture can be differentiated according to the conditions of stem cell culture. At the same time, the rotating motor is started to drive the rotating connecting rod and the rotating disk to rotate, so that the rotating disk drives the rotating main gear to rotate. By utilizing the characteristics of the meshing connection between the rotating main gear, the rotating slave gear and the meshing gear ring, not only the revolution of the rotating tripod and the rotating slave gear can be realized, but also the rotation of the rotating slave gear can be realized. The illumination lamp can realize uniform illumination of the stem cells, thereby improving the culture effect of the stem cells under illumination conditions. By setting up an independent rotating box door, when taking out the culture dish, it will not affect the culture dishes in the rest of the space, thereby improving the accuracy of the culture. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 It is a schematic diagram of the overall three-dimensional structure of the present invention;

[0025] Figure 2 It is a schematic diagram of the three-dimensional structure of the culture dish fixing mechanism in the present invention;

[0026] Figure 3 It is a schematic diagram of the three-dimensional structure of the fixed disk in the present invention;

[0027] Figure 4 It is a schematic diagram of the three-dimensional structure of the engaging gear ring and the engaging gear in the present invention;

[0028] Figure 5 It is a schematic diagram of the three-dimensional structure of the cross section of the clamping bracket in the present invention;

[0029] Figure 6 It is a schematic diagram of the three-dimensional structure of the clamping rotating plate in the present invention;

[0030] Figure 7 It is a schematic diagram of the three-dimensional structure of the fixed sleeve in the present invention;

[0031] Figure 8 It is a schematic diagram of the three-dimensional structure of the cross section of the fixing bracket in the present invention;

[0032] Fig. 9 It is a schematic diagram of the three-dimensional structure of the cross section of the rotating culture mechanism in the present invention;

[0033] Fig.10 It is a schematic diagram of the three-dimensional structure of the rotating connecting rod and the illumination lamp in the present invention;

[0034] Fig.11 It is a schematic diagram of the three-dimensional structure of the rotating slave gear and the meshing gear ring in the present invention.

[0035] In the figure: 1, culture dish fixing mechanism; 101, fixing plate; 102, mounting hole; 103, engaging gear ring; 104, engaging gear; 105, first connecting rod; 106, moving plate; 107, moving spring; 108, fixing plate; 109, second connecting rod; 110, clamping bracket; 111, clamping sliding rod; 112, clamping sliding sleeve; 113, clamping limit plate; 114, torsion spring; 115, clamping rotating shaft; 116, clamping rotating plate; 117, clamping rotating rod; 118, clamping lifting plate; 119, first through hole; 120, reset spring; 121, support ring; 122, fixing sleeve; 123, fixing hole; 124, adjustment 1. threaded rod; 125. first support plate; 126. fixed bracket; 127. locking slide; 128. adjusting gear; 129. spiral slide; 130. limit slider; 131. locking slide rod; 132. locking spring; 133. locking tilting block; 2. rotating culture mechanism; 201. incubator; 202. fixed partition plate; 203. second through hole; 204. rotating door; 205. rotating motor; 206. rotating connecting rod; 207. illumination lamp; 208. rotating disk; 209. rotating main gear; 210. rotating tripod; 211. rotating slave gear; 212. meshing gear ring; 213. second support plate; 214. door handle. DETAILED DESCRIPTION

[0036] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the implementation regulations described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0037] See also Figure 1-Figure 11 The present invention provides a technical solution: a stem cell culture device, comprising a culture dish fixing mechanism 1, and a clamping bracket 110 installed inside the culture dish fixing mechanism 1, a rotating culture mechanism 2 is arranged outside the culture dish fixing mechanism 1, and a lighting lamp 207 is arranged inside the rotating culture mechanism 2, the culture dish fixing mechanism 1 comprises a fixing plate 101, a plurality of mounting holes 102 are opened inside the fixing plate 101, and a clamping gear ring 103 is fixedly installed at the top and bottom center positions of the fixing plate 101, wherein a clamping gear 104 is clamped and connected inside the clamping gear ring 103, and a first connecting rod 105 is fixedly installed on the top of the clamping gear 104, wherein the top of the first connecting rod 105 is fixedly installed. A moving plate 106 is fixedly installed on the top of the moving plate 106, moving springs 107 are fixedly installed around the top of the moving plate 106, a fixed plate 108 is fixedly installed on the top of the moving spring 107, and a second connecting rod 109 is fixedly installed at the top center of the fixed plate 108. By pulling up the moving plate 106 and squeezing the moving spring 107, the moving plate 106 drives the first connecting rod 105 and the engaging gear 104 to move. When the engaging gear 104 is separated from the engaging gear ring 103, the fixed plate 101 can be taken out from the inside of the incubator 201, which not only can all the culture dishes on the fixed plate 101 be taken out, but also will not cause confusion in the culture dishes, which is convenient for experiments on stem cells in the culture dishes.

[0038] The clamping bracket 110 is fixedly installed inside the mounting hole 102, and a clamping sliding rod 111 is fixedly installed around the inside of the clamping bracket 110, and a clamping sliding sleeve 112 is slidably connected to the outside of the clamping sliding rod 111, and a clamping limit plate 113 is fixedly installed on the top of the clamping limit plate 113, and a torsion spring 114 is symmetrically arranged on the top of the clamping limit plate 113, and a clamping rotating shaft 115 is arranged inside the torsion spring 114, and the clamping rotating shaft 115 is rotatably connected to the clamping limit plate 113, and a clamping rotating plate 116 is fixedly installed on the outside of the clamping rotating shaft 115, and one end of the top of the torsion spring 114 is fixedly connected to the clamping rotating plate 116, and one end of the bottom of the torsion spring 114 is fixedly connected to the clamping limit plate 113, and the bottom of the clamping sliding sleeve 112 is rotatably connected There is a clamping rotating rod 117. By placing the culture dish on the first supporting plate 125 and pressing the culture dish downward, the first supporting plate 125 squeezes the reset spring 120 and drives the supporting ring 121 and the fixed sleeve 122 to move downward, so that the fixed sleeve 122 drives the clamping lifting plate 118 to move downward, so that the clamping rotating rod 117 rotates and drives the clamping sliding sleeve 112 to slide relatively on the outside of the clamping sliding rod 111. The culture dish is clamped and fixed by the clamping limiting plate 113 on the top of the clamping sliding sleeve 112, and the clamping rotating plate 116 can be rotated by the torsion spring 114, so that the clamping rotating plate 116 can fit culture dishes of different sizes, and can clamp and fix culture dishes of different diameters, thereby improving the stability of the culture dishes.

[0039] The bottom of the clamping rotating rod 117 is rotatably connected with a clamping lifting plate 118, and a first through hole 119 is provided at the inner center position of the clamping lifting plate 118. A reset spring 120 is fixedly installed at the top center position of the clamping bracket 110, and a support ring 121 is fixedly installed on the top of the reset spring 120. A fixed sleeve 122 is fixedly installed at the bottom of the support ring 121. A plurality of fixing holes 123 are provided around the outer side of the fixed sleeve 122. An adjusting threaded rod 124 is threadedly connected to the inner part of the fixed sleeve 122, and a first support plate 125 is fixedly installed on the top of the adjusting threaded rod 124. The adjusting threaded rod 124 and the support plate 125 are fixedly installed. The ring 121 is threadedly connected, and a fixed bracket 126 is fixedly installed at the bottom center position of the clamping bracket 110. The fixed bracket 126 is provided with locking grooves 127 around its interior. The bottom of the fixed bracket 126 is rotatably connected with an adjusting gear 128. The adjusting gear 128 is provided with spiral grooves 129 around its interior. The spiral groove 129 is internally slidably connected with a limited slider 130. A locking sliding rod 131 is fixedly installed on the top of the limited slider 130. The locking sliding rod 131 is slidably connected to the fixed bracket 126 through the locking groove 127. A locking spring 132 is fixedly installed on one end of the locking sliding rod 131. A locking tilting block 133 is fixedly installed at the end of the locking spring 132, and the locking tilting block 133 is engaged with the fixing sleeve 122 through the fixing hole 123. The fixing bracket 126 and the adjusting gear 128 are both slidably connected with the fixing sleeve 122, and the adjusting threaded rod 124 is slidably connected with the clamping lifting plate 118 through the first through hole 119. When the fixing sleeve 122 descends, the fixing hole 123 and the locking tilting block 133 can be used to engage with each other to fix the first support plate 125 and the clamping rotating plate 116, thereby achieving stable clamping of the culture dish, and will not cause other culture dishes to be removed. In order to prevent the remaining culture dishes from slipping off, when the culture dishes need to be taken out, the limiting slider 130 drives the locking sliding rod 131 to slide inside the fixed bracket 126 by rotating the adjusting gear 128 and under the limiting action of the spiral slide groove 129, when the locking tilting block 133 is separated from the fixing hole 123 outside the fixing sleeve 122, under the elastic force of the reset spring 120, the first support plate 125 drives the fixing sleeve 122 and the clamping lifting plate 118 to rise, and the clamping rotating rod 117 drives the clamping sliding sleeve 112 and the clamping rotating plate 116 to unfold, so that a single culture dish can be taken out from the inside of the fixed plate 101.

[0040] The rotating culture mechanism 2 includes an incubator 201, and a plurality of fixed partition plates 202 are fixedly installed inside the incubator 201, the number of the fixed partition plates 202 is three, a second through hole 203 is opened inside the fixed partition plates 202, and the fixed partition plates 202 are fixedly connected to the fixed plate 108 through the second through hole 203. A plurality of rotating door 204 are rotatably connected to the outside of the incubator 201, the number of the rotating door 204 is three, and a door handle 214 is fixedly installed on the outside of the rotating door 204. The fixed partition plates 202 are used to divide the incubator 201 into three independent spaces, so that the stem cells can be cultured differently according to the conditions of the culture.

[0041] A rotating motor 205 is fixedly installed on the inner side of the bottom of the incubator 201, and a rotating connecting rod 206 is fixedly connected to the output end of the rotating motor 205. The rotating connecting rod 206 is rotatably connected to the fixed partition plate 202. The illumination lamp 207 is fixedly installed on the bottom of the fixed partition plate 202 near the outer side of the rotating connecting rod 206. A rotating disk 208 is fixedly installed on the top of the rotating connecting rod 206. A rotating main gear 209 is fixedly installed on the top of the rotating disk 208. The top of the rotating main gear 209 is rotatably connected to a rotating tripod 210. The outer side of the rotating main gear 209 is meshedly connected to a rotating slave gear 211. The outer side of the rotating slave gear 211 is meshedly connected to a meshing gear ring 212. The meshing gear ring 212 is fixedly installed on the inner side of the top of the incubator 201, and the bottom of the meshing gear ring 212 is fixedly installed with a second The support plate 213, the rotating slave gear 211 is rotatably connected with the rotating tripod 210, and the rotating slave gear 211 is fixedly connected with the second connecting rod 109. The rotating motor 205 is started to drive the rotating connecting rod 206 and the rotating disk 208 to rotate, so that the rotating disk 208 drives the rotating main gear 209 to rotate. By utilizing the characteristics of the meshing connection between the rotating main gear 209, the rotating slave gear 211 and the meshing gear ring 212, not only the revolution of the rotating tripod 210 and the rotating slave gear 211 can be realized, but also the rotation of the rotating slave gear 211 can be realized. The illumination lamp 207 can realize uniform illumination of the stem cells, thereby improving the culture effect of the stem cells under illumination conditions. By setting an independent rotating box door 204, when taking out the culture dish, it will not affect the culture dishes in the remaining space, thereby improving the accuracy of the culture.

[0042] Working principle: Before using this stem cell culture device, you need to check the overall condition of the device to make sure it can work normally. Figure 1 - Fig.11As shown, first, by lifting the moving plate 106 upward and squeezing the moving spring 107, the moving plate 106 drives the first connecting rod 105 and the engaging gear 104 to move. When the engaging gear 104 is separated from the engaging gear ring 103, the fixed plate 101 can be taken out from the interior of the incubator 201, which not only allows all the culture dishes on the fixed plate 101 to be taken out, but also does not cause the culture dishes to be in a mess, which is convenient for conducting experiments on stem cells in the culture dishes. By placing the culture dishes on the first supporting plate 125 and pressing the culture dishes downward, the first supporting plate 125 squeezes the reset spring 120 and drives the supporting ring 121 and the fixed sleeve 122 to move downward, so that the fixed sleeve 122 drives the clamping lifting plate 118 to move downward, thereby When the clamping rotating rod 117 rotates, it drives the clamping sliding sleeve 112 to slide relatively on the outside of the clamping sliding rod 111, and the culture dish is clamped and fixed by the clamping limiting plate 113 on the top of the clamping sliding sleeve 112. The rotation of the clamping rotating plate 116 can be achieved by the torsion spring 114, so that the clamping rotating plate 116 can fit the culture dishes of different sizes, and can clamp and fix the culture dishes of different diameters, thereby improving the stability of the culture dishes. When the fixing sleeve 122 descends, the fixing hole 123 can be used to engage with the locking inclined block 133 to fix the first support plate 125 and the clamping rotating plate 116, thereby achieving stable clamping of the culture dishes, and the remaining culture dishes will not slip when other culture dishes are taken.

[0043] Secondly, when it is necessary to take the culture dish, by rotating the adjusting gear 128 and under the limiting action of the spiral slide groove 129, the limiting slider 130 drives the locking sliding rod 131 to slide inside the fixed bracket 126. When the locking tilting block 133 is separated from the fixing hole 123 on the outside of the fixed sleeve 122, under the elastic force of the reset spring 120, the first support plate 125 drives the fixed sleeve 122 and the clamping lifting plate 118 to rise, and the clamping rotating rod 117 drives the clamping sliding sleeve 112 and the clamping rotating plate 116 to unfold, so as to facilitate the removal of a single culture dish from the inside of the fixed plate 101.

[0044] Finally, the incubator 201 is divided into three independent spaces by using a fixed partition plate 202, so that the incubator 201 can be cultured differently according to the conditions of stem cell culture. At the same time, the rotating motor 205 is started to drive the rotating connecting rod 206 and the rotating plate 208 to rotate, so that the rotating plate 208 drives the rotating main gear 209 to rotate. By utilizing the meshing connection between the rotating main gear 209, the rotating slave gear 211 and the meshing gear ring 212, not only the revolution of the rotating tripod 210 and the rotating slave gear 211 can be realized, but also the rotation of the rotating slave gear 211 can be realized. The illumination lamp 207 can realize uniform illumination of the stem cells, thereby improving the culture effect of the stem cells under illumination conditions. By setting an independent rotating box door 204, when taking out the culture dish, the culture dishes in the remaining spaces will not be affected, thereby improving the accuracy of the culture.

[0045] Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments, or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A stem cell culture device, comprising a culture dish fixing mechanism (1), and a clamping bracket (110) installed inside the culture dish fixing mechanism (1); A rotating culture mechanism (2) is arranged outside the culture dish fixing mechanism (1), and an illumination lamp (207) is arranged inside the rotating culture mechanism (2); It is characterized in that Also includes: The culture dish fixing mechanism (1) comprises a fixing plate (101), a plurality of mounting holes (102) are provided inside the fixing plate (101), and a snap-fit ​​gear ring (103) is fixedly mounted at the top and bottom center positions of the fixing plate (101); The inside of the engaging gear ring (103) is engaged with an engaging gear (104), and a first connecting rod (105) is fixedly mounted on the top of the engaging gear (104); A movable plate (106) is fixedly mounted on the top of the first connecting rod (105), and movable springs (107) are fixedly mounted around the top of the movable plate (106).

2. A stem cell culture device according to claim 1, characterized in that: A fixing plate (108) is fixedly mounted on the top of the movable spring (107), a second connecting rod (109) is fixedly mounted at the top center of the fixing plate (108), the clamping bracket (110) is fixedly mounted inside the mounting hole (102), a clamping sliding rod (111) is fixedly mounted around the inside of the clamping bracket (110), and a clamping sliding sleeve (112) is slidably connected to the outer side of the clamping sliding rod (111).

3. A stem cell culture device according to claim 2, characterized in that: A clamping limit plate (113) is fixedly mounted on the top of the clamping sliding sleeve (112), a torsion spring (114) is symmetrically arranged on the top of the clamping limit plate (113), a clamping rotating shaft (115) is arranged inside the torsion spring (114), the clamping rotating shaft (115) is rotatably connected to the clamping limit plate (113), a clamping rotating plate (116) is fixedly mounted on the outer side of the clamping rotating shaft (115), a top end of the torsion spring (114) is fixedly connected to the clamping rotating plate (116), and a bottom end of the torsion spring (114) is fixedly connected to the clamping limit plate (113).

4. A stem cell culture device according to claim 3, characterized in that: The bottom of the clamping sliding sleeve (112) is rotatably connected to a clamping rotating rod (117), the bottom of the clamping rotating rod (117) is rotatably connected to a clamping lifting plate (118), a first through hole (119) is provided at the inner center position of the clamping lifting plate (118), a return spring (120) is fixedly installed at the top center position of the clamping bracket (110), a support ring (121) is fixedly installed at the top of the return spring (120), and a fixed sleeve (122) is fixedly installed at the bottom of the support ring (121).

5. A stem cell culture device according to claim 4, characterized in that: A plurality of fixing holes (123) are formed around the outer side of the fixing sleeve (122); an adjusting threaded rod (124) is threadedly connected to the interior of the fixing sleeve (122); a first supporting plate (125) is fixedly mounted on the top of the adjusting threaded rod (124); the adjusting threaded rod (124) is threadedly connected to the supporting ring (121); and a fixing bracket (126) is fixedly mounted at the bottom center of the clamping bracket (110).

6. A stem cell culture device according to claim 5, characterized in that: The fixed bracket (126) is provided with locking grooves (127) on the inner periphery thereof; the bottom of the fixed bracket (126) is rotatably connected to an adjusting gear (128); the inner periphery of the adjusting gear (128) is provided with spiral grooves (129); the inner periphery of the spiral groove (129) is slidably connected to a limit slider (130); a locking sliding rod (131) is fixedly installed on the top of the limit slider (130); the locking sliding rod (131) is slidably connected to the fixed bracket (126) via the locking groove (127).

7. A stem cell culture device according to claim 6, characterized in that: A locking spring (132) is fixedly mounted on one end of the locking sliding rod (131), a locking tilting block (133) is fixedly mounted on the end of the locking spring (132), the locking tilting block (133) is snap-connected to the fixing sleeve (122) via the fixing hole (123), the fixing bracket (126) and the adjusting gear (128) are both slidably connected to the fixing sleeve (122), and the adjusting threaded rod (124) is slidably connected to the clamping lifting plate (118) via the first through hole (119).

8. The stem cell culture device according to claim 2, characterized in that: The rotating culture mechanism (2) comprises a culture box (201), a plurality of fixed partition plates (202) are fixedly installed inside the culture box (201), the number of the fixed partition plates (202) is three, a second through hole (203) is opened inside the fixed partition plates (202), the fixed partition plates (202) are fixedly connected to the fixed plate (108) via the second through hole (203), a plurality of rotating box doors (204) are rotationally connected to the outside of the culture box (201), the number of the rotating box doors (204) is three, a box door handle (214) is fixedly installed on the outside of the rotating box door (204), and a rotating motor (205) is fixedly installed on the inside of the bottom of the culture box (201).

9. A stem cell culture device according to claim 8, characterized in that: The output end of the rotating motor (205) is fixedly connected to a rotating connecting rod (206), and the rotating connecting rod (206) is rotatably connected to the fixed partition plate (202). The illumination lamp (207) is fixedly mounted on the bottom of the fixed partition plate (202) near the outer side of the rotating connecting rod (206). A rotating disk (208) is fixedly mounted on the top of the rotating connecting rod (206), and a rotating main gear (209) is fixedly mounted on the top of the rotating disk (208). The top of the rotating main gear (209) is rotatably connected to a rotating three-axis gear (208). The tripod (210) comprises a rotating main gear (209) meshingly connected with a rotating slave gear (211) on its outer side, a meshing gear ring (212) meshingly connected with the rotating slave gear (211) on its outer side, the meshing gear ring (212) being fixedly mounted on the inner side of the top of the incubator (201), a second support plate (213) being fixedly mounted on the bottom of the meshing gear ring (212), the rotating slave gear (211) being rotationally connected to the rotating tripod (210), and the rotating slave gear (211) being fixedly connected to the second connecting rod (109).

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