Pesticide composition and application thereof

Abstract

The invention belongs to the field of pesticide sterilization, and discloses a pesticide composition and application thereof. Effective components of the bactericidal composition comprise an active component A and an active component B, the active component A is a compound represented by a formula I: # imgabs0, and the active component B is any one of penflufen and sedaxane; the mass ratio of the active component A to the active component B is (1: 35)-(35: 1). The pesticide composition disclosed by the invention has a broad-spectrum bactericidal effect, the use amount and use cost of effective components are effectively reduced, and generation and development of resistance are delayed.

Description

Technical Field

[0001] The present invention relates to the technical field of pesticide sterilization, and in particular to a pesticide composition and application thereof. Background Art

[0002] The compound of formula I is a methoxyacrylate fungicide developed by Sumitomo Chemical Co., Ltd., CAS registration number: 2454319-63-0, Chinese chemical name: (2Z)-3-methoxy-2-[(4-methyl[1,1'-biphenyl]-3-yl)oxy]prop-2-enoic acid methyl ester, the chemical structure is as follows:

[0003]

[0004] Sedaxane, ISO generic name: sedaxane, belongs to the SDHI inhibitor fungicide. It acts on succinate dehydrogenase, one of the hubs connecting oxidative phosphorylation and electron transfer in bacteria, leading to tricarboxylic acid cycle disorders, hindering their energy metabolism, and then inhibiting the growth of pathogens, leading to their death, thereby achieving the purpose of disease prevention and control. Sedaxane can penetrate from seeds to the surrounding soil, thus forming a protective circle for seeds, roots and base stems. It has good mobility in different soil types and can be evenly distributed in the root system of crops.

[0005] Penflufen, ISO common name: penflufen, chemical name: N-[2-(1,3-dimethylbutyl)phenyl]-5-1,3-dimethyl-1H-pyrazole-4-carboxamide, CAS registration number: 494793-67-8. Penflufen is a pyrazoleamide fungicide developed by Bayer. It has good activity against a variety of plant pathogenic fungi. The fungicide is a succinate dehydrogenase inhibitor, which mainly acts on the electron transfer complex II of the respiratory chain to block energy metabolism. Penflufen has systemic, preventive and therapeutic effects and has a long lasting effect. It is mainly used as a fungicidal seed treatment agent. After seed treatment, the agent penetrates into the germinating seeds and is transmitted to the entire plant through the xylem of the young plant, thereby protecting the growing seedlings.

[0006] Long-term use of a single agent and unreasonable application methods make it easy for pathogens to develop drug resistance, resulting in decreased efficacy and disease outbreaks, which in turn increases the dosage of the drug, forming a vicious cycle, increasing drug costs, and exacerbating environmental pollution. The present invention rationally compounded the compound of formula I with trifloxystrobin and trifloxystrobin, analyzed the toxicity of different fungicide combinations to wheat sheath blight and corn head smut, and compared the application effects of different treatments in field trials, in order to provide a theoretical basis for the chemical control of wheat sheath blight and corn head smut. Summary of the invention

[0007] Based on the above situation, the purpose of the present invention is to provide a pesticide composition and its preparation, which are mainly used for preventing and controlling plant fungal diseases. The composition or its preparation can enhance the efficacy, reduce the dosage, and at the same time can prolong the lasting effect and delay the development of drug resistance.

[0008] In order to achieve the above object, the present invention adopts the following technical scheme: a pesticide composition, the pesticide composition comprises an active ingredient A and an active ingredient B, and the active ingredient A is a compound of formula I: The active ingredient B is any one of trifloxystrobin and trifloxystrobin; the mass ratio of the active ingredient A to the active ingredient B is 1:35 to 35:1, or any value within the above numerical range;

[0009] Furthermore, the active ingredient B is pentofenac, and the mass ratio of the active ingredient A to the active ingredient B is 1:22 to 25:1, or any value within the above numerical range;

[0010] The active ingredient B is fluazifop, and the mass ratio of the active ingredient A to the active ingredient B is 1:35 to 20:1, or any value within the above numerical range.

[0011] Furthermore, the pesticide composition according to claim 1 is characterized in that the active ingredient B is penflufenacil, and the mass ratio of the active ingredient A to the active ingredient B is 1:15 to 18:1, or any value within the above numerical range;

[0012] The active ingredient B is fluazifop, and the mass ratio of the active ingredient A to the active ingredient B is 1:30 to 20:1, or any value within the above numerical range;

[0013] Furthermore, the active ingredient B is pentocyclanil, and the mass ratio of the active ingredient A to the active ingredient B is 1:14 to 15:1, or any value within the above numerical range;

[0014] The active ingredient B is fluazifop, and the mass ratio of the active ingredient A to the active ingredient B is 1:16 to 18:1, or any value within the above numerical range;

[0015] Furthermore, based on the total weight of the pesticide composition being 100 wt%, the sum of the contents of the active ingredient A and the active ingredient B in the pesticide composition is 0.1 to 95 wt%, preferably 2 to 80 wt%.

[0016] Furthermore, the pesticide composition also includes an adjuvant, which is selected from one or more of a wetting agent, a dispersant, an emulsifier, a thickener, a disintegrant, an antifreeze agent, a defoaming agent, a solvent, a preservative, a stabilizer, a synergist or a carrier.

[0017] Further, the formulation of the pesticide composition is any one of emulsifiable concentrate, water emulsion, microemulsion, suspension, wettable powder, seed treatment suspension or water dispersible granules;

[0018] Furthermore, the pesticide composition is in the form of a seed treatment suspension.

[0019] The present invention also discloses the use of the above pesticide composition in preventing and controlling plant diseases;

[0020] Furthermore, the plant disease is a wheat disease, a rice disease, a corn disease or a potato disease caused by fungi.

[0021] Further, the diseases are potato black mole, wheat loose smut, wheat sheath blight, wheat head blight, corn leaf spot, corn smut, corn head smut, corn sheath blight, rice sheath blight, rice seedling blight, rice seedling rot, and rice damping off;

[0022] Furthermore, the diseases are wheat sheath blight and corn head smut.

[0023] Compared with the prior art, the beneficial effects of the technical solution of the present invention are as follows:

[0024] (1) The pesticide composition of the present invention combines the compound of formula I with different mechanisms of action with either pentozolin or pentozolin, thereby increasing the fungicidal activity and expanding the scope of application;

[0025] (2) The pesticide composition of the present invention is safe and environmentally friendly, has broad development prospects, and can be applied once during the entire growth period of crops, thereby reducing the amount of pesticides used and lowering agricultural costs;

[0026] (3) The pesticide composition of the present invention has significant protective effect, a long lasting effect, a broad bactericidal spectrum, and can delay the development of drug resistance in harmful bacteria. DETAILED DESCRIPTION

[0027] In order to make the technical solutions, objectives and advantages of the present invention more clearly understood, the present invention is described with the following specific embodiments, but the present invention can be implemented in various forms and should not be limited by the embodiments described herein.

[0028] Preparation method of seed treatment suspension: Preparation method of seed treatment suspension: According to the formula ratio, the active ingredients, additives and water are mixed and stirred evenly through high shear, and then sanded by a grinder for 2.5 hours to make the average particle size reach 1-5 microns, and finally thickeners, preservatives and film-forming agents are added and continued to be sheared and stirred evenly to obtain the seed treatment suspension.

[0029] Preparation Example 1: 24% Formula I compound·flufenamid seed treatment suspension (1:7)

[0030] Calculated by weight percentage, 3% of the compound of formula I, 21% of fluopicolide, 5% of fatty amine polyoxyethylene ether phosphate, 2% of fatty alcohol polyoxyethylene ether, 2% of fatty alcohol ethylene oxide-propylene oxide copolymer, 3% of polycarboxylic acid sodium salt, 1% of magnesium aluminum silicate, 0.25% of xanthan gum, 2% of polyacrylic acid emulsion, 5% of rose red pigment, 5% of ethylene glycol, 0.5% of silicone defoamer, 0.5% of sodium benzoate, and deionized water make up the balance.

[0031] Preparation Example 2: 21% Formula I compound·flufenamid seed treatment suspension (1:2)

[0032] Calculated by weight percentage, 7% compound of formula I, 14% fluopicolide, 2% castor oil polyoxyethylene ether, 1% sodium lignin sulfonate, 5% isotridecyl alcohol polyoxyethylene ether, 2% styrene phenol polyoxyethylene ether sulfate, 1% magnesium aluminum silicate, 0.25% xanthan gum, 1% polyacrylic acid emulsion, 5% rose red pigment, 5% propylene glycol, 0.5% silicone defoamer, 0.15% benzisothiazolinone potassium, and deionized water make up the balance.

[0033] Preparation Example 3: 30% Formula I compound·flufenamid seed treatment suspension (5:1)

[0034] Calculated by weight percentage, 25% of the compound of formula I, 5% of fluopicolide, 1% of sorbitan oleate polyoxyethylene ether, 1% of arylphenol polyoxyethylene ether phosphate, 2% of sodium lauryl sulfate, 1% of polyacrylic acid emulsion, 0.25% of xanthan gum, 6% of rose red pigment, 5% of ethylene glycol, 1% of magnesium aluminum silicate, 0.5% of silicone defoamer, 1% of sodium sorbate, and deionized water make up the balance.

[0035] Preparation Example 4: 25% Formula I compound·flufenamid seed treatment suspension (2:3)

[0036] Calculated by weight percentage, 10% compound of formula I, 15% fluopicolide, 2% polyoxyethylene sorbitan monooleate, 5% EO / PO block copolymer, 1% phenethylphenol polyoxyethylene phosphate, 1% naphthalenesulfonate formaldehyde condensate, 1% polyacrylic acid emulsion, 0.25% xanthan gum, 6% rose red pigment, 5% ethylene glycol, 1% magnesium aluminum silicate, 0.5% silicone defoamer, 0.5% isothiazolinone, and deionized water makes up the balance.

[0037] Preparation Example 5: 18% Formula I compound·flufenamid seed treatment suspension (8:1)

[0038] By weight percentage, 16% of the compound of formula I, 2% of fluopicolide, 3% of alkylphenol formaldehyde resin polyoxyethylene ether, 3% of EO / PO block copolymer, 2% of Guerbet alcohol polyoxyethylene ether, 2% of naphthalenesulfonate formaldehyde condensate, 1% of polyethylene glycol, 0.25% of xanthan gum, 1% of polyacrylic acid emulsion, 4% of rose red pigment, 5% of ethylene glycol, 1% of magnesium aluminum silicate, 0.5% of silicone defoamer, 0.2% of potassium benzoate, and deionized water make up the balance.

[0039] Preparation Example 6: 22% Formula I compound·fluazifop-dopamine seed treatment suspension (10:1)

[0040] Calculated by weight percentage, 20% compound of formula I, 2% fluazifop, 3% alkylphenol polyoxyethylene ether, 2% fatty alcohol polyoxyethylene ether sulfate, 0.5% sodium lignin sulfonate, 1% polyacrylic acid emulsion, 0.25% xanthan gum, 5% rose red pigment, 5% propylene glycol, 1% magnesium aluminum silicate, 0.5% silicone defoamer, 1% benzisothiazolinone potassium, and deionized water to make up the balance.

[0041] Preparation Example 7: 27% Formula I compound·fluazifop-dopamine seed treatment suspension (2:1)

[0042] By weight percentage, 18% of the compound of formula I, 9% of fluazifop, 3% of castor oil polyoxyethylene ether, 3% of alkylphenol polyoxyethylene ether, 1% of styrenephenol polyoxyethylene ether phosphate, 2% of sodium alginate, 2% of sodium lauryl sulfate, 1% of sodium carboxymethyl starch, 0.1% of xanthan gum, 5% of rose red pigment, 5% of propylene glycol, 1% of magnesium aluminum silicate, 0.5% of silicone defoamer, 1% of sodium sorbate, and deionized water make up the balance.

[0043] Preparation Example 8: 36% Formula I compound·fluazifop-dopamine seed treatment suspension (1:8)

[0044] By weight percentage, 4% of the compound of formula I, 32% of fluazifop, 3% of EO / PO block copolymer, 2% of fatty alcohol polyoxyethylene ether sulfate, 2% of fatty alcohol polyoxyethylene ether, 1% of polyacrylic acid emulsion, 0.2% of xanthan gum, 5% of rose red pigment, 5% of ethylene glycol, 1% of magnesium aluminum silicate, 0.5% of silicone defoamer, 1% of sodium p-hydroxybenzoate, and deionized water to make up the balance;

[0045] Preparation Example 9: 30% Formula I compound·fluazifop-dopamine seed treatment suspension (5:1)

[0046] By weight percentage, 25% of the compound of formula I, 5% of fluazifop, 5% of ethylene glycol oxyethylene polyoxypropylene ether, 2% of isomeric tridecyl alcohol polyoxyethylene ether, 1% of sodium lignin sulfonate, 3% of tristyrylphenol ethoxylate phosphate, 1% of sodium lignin sulfonate, 2% of polyvinyl alcohol, 0.25% of xanthan gum, 5% of rose red pigment, 5% of propylene glycol, 1% of magnesium aluminum silicate, 0.5% of silicone defoamer, 2% of kason, and deionized water are used to make up the balance.

[0047] Preparation Example 10: 14% Formula I compound·fluazifop-dopamine seed treatment suspension (2:5)

[0048] Calculated by weight percentage, 4% of the compound of formula I, 10% of fluazifop, 3% of isotridecyl alcohol polyoxyethylene ether, 3% of tristyrylphenol ethoxylate phosphate, 1% of sodium alkyl polyoxyethylene ether sulfonate, 1% of polyacrylic acid emulsion, 0.3% of xanthan gum, 5% of rose red pigment, 5% of propylene glycol, 1% of magnesium aluminum silicate, 0.5% of silicone defoamer, 1% of sodium benzoate, and deionized water make up the balance.

[0049] Indoor toxicity test

[0050] Example 1: Indoor joint action test of the compound of formula I with pentozolin and pentozolin against wheat sheath blight

[0051] Test target: Rhizoctonia cerealis.

[0052] Preparation of test materials: Transfer the pathogens stored in the refrigerator to PDA culture medium plates and culture them at 28℃ for 2 days before use.

[0053] Test agents: technical drug of the compound of formula I, technical drug of pentocyclanil, technical drug of pentocyclanil.

[0054] Preparation of drug mother solution: After dissolving the original drugs in appropriate solvents to prepare high-concentration mother solutions, the single agents and mixtures are diluted into 5 series of mass concentrations with 0.1% Tween 80 aqueous solution.

[0055] Test repetition: 4 culture dishes for each concentration of the test drug, 1 culture dish for each repetition, for a total of 4 repetitions, and a 0.1% Tween 80 aqueous solution without drug was used as a blank control.

[0056] Drug treatment: Under aseptic operation conditions, add the pre-melted sterilized culture medium to the sterile conical flask according to the test treatment, and quantitatively draw the drug solution from low concentration to high concentration, add it to the above conical flask respectively, and shake it thoroughly. Then pour an equal amount into 4 culture media with a diameter of 9 cm to make drug-containing plates of corresponding concentrations. Set the treatment without drug as blank control, and repeat each treatment 4 times.

[0057] Inoculation: Cut the pre-cultured Rhizoctonia graminearum from the edge of the colony with a sterile puncher under sterile conditions, and then inoculate the cakes in the center of the drug-containing plate with an inoculator, with the mycelium facing up, and cover with a cap. After inoculation, the culture dish is cultured at a constant temperature of 28°C.

[0058] Investigation: Investigate the growth of pathogenic fungi based on the growth of colonies in the blank control culture dish. Use a caliper to measure the diameter of the colonies in centimeters. Use the cross method to measure the diameter of each colony vertically once and take the average value.

[0059] Data statistics and analysis: According to the survey results, the mycelial growth inhibition rate of each treatment concentration on the target bacteria was calculated. The unit is percentage (%) and the calculation result is rounded to two decimal places.

[0060] D=D1-D2

[0061] Where:

[0062] D——colony growth diameter;

[0063] D1——colony diameter;

[0064] D2——diameter of mushroom cake.

[0065]

[0066] Where:

[0067] I——hyphae growth inhibition rate;

[0068] D0——blank control colony growth diameter;

[0069] D T ——The growth diameter of the colony after treatment with chemicals.

[0070] Analyze with statistical analysis system to find the toxicity regression line and EC 50 Value, to evaluate the activity of the test agent on the biological test material.

[0071] Sun Yunpei method: The synergistic effect of mixed drugs is evaluated based on the co-toxicity coefficient (CTC). A co-toxicity coefficient of ≥120 indicates a synergistic effect; CTC≤80 indicates an antagonistic effect; and 80<CTC<120 indicates an additive effect.

[0072] Calculation of the co-toxicity coefficient (CTC value) of the mixture:

[0073]

[0074] Where:

[0075] ATI——Actual Toxicity Index of Mixture;

[0076] S——EC of standard medicine 50 , the unit is milligrams per liter (mg / L);

[0077] M——EC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0078] TTI=TI A ×P A +TI B ×P B

[0079] Where:

[0080] TTI – Theoretical Toxicity Index of Mixtures;

[0081] TI A ——A: toxicity index of the agent;

[0082] P A ——The percentage of agent A in the mixture, in percentage (%);

[0083] TI B ——Toxicity index of agent B;

[0084] P B ——The percentage of agent B in the mixture, in percentage (%).

[0085]

[0086] Where:

[0087] CTC – Co-toxicity coefficient;

[0088] ATI——Actual Toxicity Index of Mixture;

[0089] TTI - Theoretical Toxicity Index of Mixture.

[0090] The test results are shown in the following table:

[0091] Test results:

[0092] Table 1: Results of indoor combined action test of compound of formula I and pentozolin against wheat sheath blight

[0093]

[0094] Table 2: Results of indoor combined action test of compound of formula I and fluazifop-butyl against wheat sheath blight

[0095]

[0096]

[0097] It can be seen from the indoor activity tests in Tables 1 and 2 that the compound of formula I in combination with either pentamethylenediamine or pentamethylenediamine exhibits a good inhibitory effect on the growth of mycelium of wheat sheath blight pathogen.

[0098] From the test results in Table 1, it can be seen that the tested agent compound of formula I and pentozolin have a good control effect on wheat sheath blight pathogens, and its EC 50 The co-toxicity coefficients of the compound of formula I and pentozopyra are greater than 120 in the mass ratio of 1:22 to 25:1, which has a synergistic effect on wheat sheath blight; the co-toxicity coefficients of the compound of formula I and pentozopyra are greater than 130 in the mass ratio of 1:14 to 8:1, which has a significant synergistic effect on wheat sheath blight; among them, the co-toxicity coefficient of the compound of formula I and pentozopyra is the largest when the mass ratio of 1:7 is used to compound pentozopyra, and the synergistic effect is the most significant.

[0099] From the test results in Table 2, it can be seen that the tested agent compound of formula I and fluazifop-butyl have a good control effect on wheat sheath blight pathogens, and its EC 50 The co-toxicity coefficients of the compound of formula I and fluzoxacillin in the mass ratio range of 1:35 to 20:1 were all greater than 120, which had a synergistic effect on wheat sheath blight; the co-toxicity coefficients of the compound of formula I and fluzoxacillin in the mass ratio range of 1:25 to 18:1 were all greater than 130, which had a significant synergistic effect on wheat sheath blight; among them, the co-toxicity coefficient of the compound of formula I and fluzoxacillin in the mass ratio of 2:1 was the largest, and the synergistic effect was the most significant.

[0100] Example 2: Indoor joint action test of the compound of formula I with pentozolin and pentozolin against corn head smut

[0101] Test agents: technical drug of the compound of formula I, technical drug of pentocyclanil, technical drug of pentocyclanil.

[0102] Test pathogen: Maize head smut pathogen (Sphacelotheca reiliana).

[0103] Preparation of medicine: dissolve the above raw medicine with a suitable solvent, and then dilute with 0.1% Tween 80 aqueous solution; prepare single-dose mother liquor respectively, and set a series of mass concentrations according to the purpose of mixing and the activity of the medicine.

[0104] Preparation of spore suspension: Weigh 3 mg of powder of head smut fungus, add 15 ml of 1% CuSO4 solution and soak for 24 hours, then centrifuge at 4000r / min for 5 minutes, discard the supernatant, add sterile water, shake well and centrifuge again, and use sterile water to prepare spore suspension from the spore precipitate. Adjust the spore number to about 50 in each field of view for observation under a low-power microscope (10×20).

[0105] Preparation of culture medium: After melting the PDA culture medium in a microwave oven, cool it to about 50°C. According to the principle of low concentration to high concentration, take 1mL of the prepared drug solution to be tested and 9mL of PDA culture medium and add them to a culture dish with a diameter of 9cm. Mix well to prepare drug-containing plates of corresponding concentrations. Repeat each treatment 4 times.

[0106] Inoculation and culture: 0.1 ml of spore suspension was added to each treatment on the prepared PDA plate, and the plate was scraped and evenly spread, and then placed in a 28°C incubator. After 72 hours of culture, when the control germination rate reached the maximum, the spore germination in each agent treatment was observed. A total of 300 spores were investigated in 3 fields of view in each PDA plate.

[0107] Calculation results:

[0108]

[0109] Statistical analysis: Regression analysis was performed based on the logarithmic values ​​of each agent concentration and the corresponding probability values ​​of spore germination inhibition rate to calculate the EC of each agent. 50 Equivalence, evaluate the activity of the test agent on the biological test material.

[0110] Sun Yunpei method: The synergistic effect of mixed drugs is evaluated based on the co-toxicity coefficient (CTC). A co-toxicity coefficient of ≥120 indicates a synergistic effect; CTC≤80 indicates an antagonistic effect; and 80<CTC<120 indicates an additive effect.

[0111] Calculation of the co-toxicity coefficient (CTC value) of the mixture:

[0112]

[0113] Where:

[0114] ATI——Actual Toxicity Index of Mixture;

[0115] S——EC of standard medicine 50 , the unit is milligrams per liter (mg / L);

[0116] M——EC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0117] TTI=TI A ×PA +TI B ×P B

[0118] Where:

[0119] TTI – Theoretical Toxicity Index of Mixtures;

[0120] TI A ——A: toxicity index of the agent;

[0121] P A ——The percentage of agent A in the mixture, in percentage (%);

[0122] TI B ——Toxicity index of agent B;

[0123] P B ——The percentage of agent B in the mixture, in percentage (%).

[0124]

[0125] Where:

[0126] CTC – Co-toxicity coefficient;

[0127] ATI——Actual Toxicity Index of Mixture;

[0128] TTI - Theoretical Toxicity Index of Mixture.

[0129] The test results are shown in the following table:

[0130] Table 3: Results of indoor combined action test of compound of formula I and pentrioxam mixed with corn head smut

[0131]

[0132] Table 4: Indoor joint action test of the compound of formula I and pentocyclam against corn head smut

[0133]

[0134] It can be seen from the indoor activity tests in Tables 3 and 4 that the compound of formula I in combination with either pentozolin or pentozolin exhibited a good inhibitory effect on the spore germination of corn head smut fungus.

[0135] From the test results in Table 3, it can be seen that the compound of formula I and pentrioxadone have a good control effect on corn head smut pathogens. 50The co-toxicity coefficients of the compound of formula I and pentozopyra are greater than 120 in the mass ratio of 1:22 to 25:1, which has a synergistic effect on corn head smut; the co-toxicity coefficients of the compound of formula I and pentozopyra are greater than 130 in the mass ratio of 1:15 to 18:1, which has a significant synergistic effect on corn head smut; among them, the co-toxicity coefficient of the compound of formula I and pentozopyra is the largest when the mass ratio of 2:3 is used to compound pentozopyra, and the synergistic effect is the most significant.

[0136] From the test results in Table 4, it can be seen that the tested agent compound of formula I and fluazifop-butyl have a good control effect on corn head smut pathogens. 50 The co-toxicity coefficients of the compound of formula I and fluzoxacillin in the mass ratio range of 1:30 to 20:1 were all greater than 120, which had a synergistic effect on corn head smut; the co-toxicity coefficients of the compound of formula I and fluzoxacillin in the mass ratio range of 1:10 to 10:1 were all greater than 130, which had a significant synergistic effect on corn head smut; among them, the co-toxicity coefficient of the compound of formula I and fluzoxacillin in the mass ratio of 2:5 was the largest, and the synergistic effect was the most significant.

[0137] Example 3: Field test on efficacy of drugs for controlling wheat sheath blight

[0138] The test site was Baimiao Village, Gebukou Township, Yuanyang County, Henan Province. The test period was from wheat sowing to wheat harvesting. All cultivation and management conditions in the test area were uniform. Wheat sheath blight occurred widely and evenly over the years.

[0139] Test target: Wheat sheath blight.

[0140] Experimental crops: wheat (Yumai 49).

[0141] Experimental design: 1 day before sowing, the test agent was mixed with water according to the dosage in the table below and the wheat seeds were dried for later use. A water control was set up. Each treatment was repeated 4 times and arranged in random blocks. The experiment was sown with a manual seeder on the morning of October 18.

[0142] Experimental investigation: The occurrence of wheat sheath blight was investigated during the jointing stage of wheat. Samples were taken at 5 fixed points on the diagonal of each plot, and 100 plants were checked at each point. The disease index and control effect were calculated according to the following grading method.

[0143] Grading Standard:

[0144] Level 0, no disease;

[0145] Level 1, the leaf sheaths are diseased but the stems are not diseased;

[0146] Level 3: The leaf sheath is infected and invades the stem, but the lesions on the stem are less than 1 / 2 of the stem;

[0147] Level 5: The lesions on the stems encircle more than 1 / 2 of the stems, but the stems do not fall or break;

[0148] Level 7: death, lodging, and dry white ears.

[0149] Yield survey: measure yield during wheat harvest and calculate yield increase rate.

[0150] Calculation method:

[0151]

[0152]

[0153] The results of the field efficacy test are as follows:

[0154] Table 5: Results of field trials on efficacy of drugs for controlling wheat sheath blight

[0155]

[0156] As can be seen from Table 5, the compound preparation treatment has an obvious overall prevention and control effect on wheat sheath blight. The disease index of wheat sheath blight in the test field after treatment is significantly reduced, and the compound preparation also has a good yield-increasing effect on wheat seed treatment.

[0157] Example 4: Field test on efficacy of drugs for controlling corn head smut

[0158] Test location: Experimental field in Zhaizi Village, Xujialou Township, Taishan District, Tai'an City, Shandong Province. The test field is sandy loam with medium soil fertility.

[0159] Test target: Maize head smut.

[0160] Experimental crops: corn (Zheng Dan 958).

[0161] Experimental design: Weigh the designed dosage of each treatment and put it into a plastic bag. Hold the bag tightly to fill the bag with air and shake it thoroughly until the seeds are evenly coated. Dry in the shade and then sow. Sow seeds on April 26.

[0162] Disease investigation: After all ears emerge and symptoms are obvious, investigate the results of corn head smut test. Randomly investigate 500 plants in each plot, record the total number of plants and the number of diseased plants, and calculate the incidence rate and control effect.

[0163] Yield determination: When corn is mature, harvest it in the entire plot, dry it, thresh it, and weigh it individually to calculate the yield and yield increase rate.

[0164] Calculation method of drug efficacy:

[0165]

[0166] Yield increase rate (%) = empty white yield of the treatment area - empty white yield of the control area × 100

[0167] The results of the field efficacy test are as follows:

[0168] Table 6: Field test results of efficacy of drugs for controlling corn head smut

[0169]

[0170] As shown in Table 6, the compound suspension seed coating agent had a good control effect on corn head smut at the experimental dosage, and the yield of each treatment was significantly higher than that of the blank control; the increase in yield was 7.46% to 9.63%.

[0171] It can be seen from indoor toxicity determination and field tests that the pesticide composition of the present invention shows good control effect on crop diseases, especially wheat sheath blight and corn head smut. The pesticide composition or its preparation obtained by compounding of the present invention has significant control effect, and is superior to a single agent in delaying the generation of drug resistance and prolonging drug retention. In addition, no compound agent was found to cause drug damage to crops in the test, indicating that when the bactericidal synergistic effect of the obtained pesticide composition or preparation is improved, the production cost and use cost can be reduced, and it is safe for crops.

[0172] Although the present invention has been described in detail above with general descriptions and specific implementation schemes, it is obvious to those skilled in the art that some modifications or improvements can be made on the basis of the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention are within the scope of protection claimed by the present invention.

Claims

1. A pesticide composition, characterized in that: The pesticide composition comprises active ingredient A and active ingredient B, wherein the active ingredient A is a compound of formula I: The active ingredient B is any one of pentozolin and pentozolin; the mass ratio of the active ingredient A to the active ingredient B is 1:35 to 35:

1.

2. The pesticide composition according to claim 1, characterized in that The active ingredient B is trifloxystrobin, and the mass ratio of the active ingredient A to the active ingredient B is 1:22 to 25:1; The active ingredient B is fluazifop, and the mass ratio of the active ingredient A to the active ingredient B is 1:35 to 20:

1.

3. The pesticide composition according to claim 2, characterized in that The pesticide composition according to claim 1, characterized in that the active ingredient B is trifloxystrobin, and the mass ratio of the active ingredient A to the active ingredient B is 1:15 to 18:1; The active ingredient B is fluazifop, and the mass ratio of the active ingredient A to the active ingredient B is 1:30 to 20:

1.

4. The pesticide composition according to claim 3, characterized in that The active ingredient B is trifloxystrobin, and the mass ratio of the active ingredient A to the active ingredient B is 1:14 to 15:1; The active ingredient B is fluazifop, and the mass ratio of the active ingredient A to the active ingredient B is 1:16 to 18:

1.

5. The pesticide composition according to claim 1, characterized in that Based on the total weight of the pesticide composition being 100 wt %, the sum of the contents of the active ingredient A and the active ingredient B in the pesticide composition is 0.1 to 95 wt %, preferably 2 to 80 wt %.

6. The pesticide composition according to claim 1, characterized in that The pesticide composition further comprises an adjuvant, which is selected from one or more of a wetting agent, a dispersant, an emulsifier, a thickener, a disintegrant, an antifreeze agent, a defoaming agent, a solvent, a preservative, a stabilizer, a synergist or a carrier.

7. The pesticide composition according to claim 1, characterized in that The pesticide composition is in the form of any one of emulsifiable concentrate, water emulsion, microemulsion, suspension, wettable powder, seed treatment suspension or water dispersible granules; Preferably, the pesticide composition is in the form of a seed treatment suspension.

8. Use of the pesticide composition according to any one of claims 1 to 7 in preventing and controlling plant diseases.

9. The use according to claim 8, characterized in that: The plant diseases are wheat diseases, rice diseases, corn diseases or potato diseases caused by fungi.

10. The use according to claim 9, characterized in that: The diseases are potato black mole, wheat loose smut, wheat sheath blight, wheat fusarium head blight, corn leaf spot, corn smut, corn head smut, corn sheath blight, rice sheath blight, rice seedling blight, rice rot, rice seedling rot and rice damping off.

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