A bactericidal composition and its use

Abstract

The present application relates to a kind of fungicide composition and its application, comprising active ingredient A and active ingredient B, the active ingredient A is the compound shown in formula (I), and active ingredient B is any one of Feneptamidoquin, Fenopyramid, the mass ratio of active ingredient A and active ingredient B is 1:36~20:1.The fungicide composition or its preparation of the present application has significant synergistic effect on the disease caused by a variety of fungi, is significantly higher than the prevention and treatment effect of single component, the effective period is long, can delay the development of drug resistance simultaneously, is pollution-free to environment, is safe to natural enemy and crop.

Description

Technical Field

[0001] This invention relates to the field of pesticides and fungicides, specifically to a fungicidal composition and its application. Background Technology

[0002] Bifemetstrobine is a methoxyacrylate fungicide developed by Sumitomo Chemical Co., Ltd. Its CAS Registry Number is 2454319-63-0, and its Chinese chemical name is (2Z)-3-methoxy-2-[(4-methyl[1,1'-biphenyl]-3-yl)oxy]prop-2-enoic acid methyl ester. Its chemical structure is as follows:

[0003]

[0004] Feneptamidoquin is a quinoline carboxamide fungicide developed by Syngenta. It has a broad spectrum of activity, is safe and highly effective, and can effectively control a variety of fungal diseases. Its CAS Registry Number is 2132414-06-1 (racemic 2132414-04-9), and its English chemical name is N-(2R)-[1,3-dimethyl-1-(phenylmethyl)butyl]-8-fluoro-3-quinolinecarboxamide. Its chemical structure is as follows:

[0005]

[0006] Fenopyramid is a pyrazole carboxamide fungicide developed by Shandong Zhongnong United Biotechnology Co., Ltd. Its CAS Registry Number is 2344721-61-3, and its Chinese chemical name is N-{2-[2,4-bis(trifluoromethyl)phenoxy]phenyl}-3-(difluoromethyl)-1-methyl-1H-pyrazole-4-carboxamide. Its chemical structure is as follows:

[0007]

[0008] Currently, chemical pesticides remain the mainstay of crop disease control. However, prolonged use of fungicides can lead to cross-resistance, resulting in a significant reduction in efficacy. Combining two or more fungicides to create a synergistic effect and improve efficacy is a primary solution to this problem. Furthermore, combinations of compound (I) with Feneptamidoquin and Fenopyramid have not yet been reported. Summary of the Invention

[0009] Based on the above, the present invention aims to provide a fungicidal composition and its formulation, mainly used for the prevention and control of plant fungal diseases. The composition or its formulation can enhance efficacy, reduce dosage, and has a significant synergistic effect under certain mass ratios. At the same time, it can prolong the duration of efficacy and delay the development of drug resistance.

[0010] To achieve the above objectives, the following technical solution is provided: a bactericidal composition comprising active ingredient A and active ingredient B, wherein active ingredient A is a compound of formula (I), with the following chemical structural formula:

[0011] Active ingredient B is either Feneptamidoquin or Fenopyramid;

[0012] Furthermore, the mass ratio of active ingredient A to active ingredient B is 1:36 to 20:1, or any value between the above values;

[0013] Furthermore, the mass ratio of active ingredient A to active ingredient B is 1:24 to 20:1;

[0014] Furthermore, the mass ratio of the compound of formula (I) to Feneptamidoquin is 1:20 to 18:1;

[0015] Furthermore, the mass ratio of the compound of formula (I) to Feneptamidoquin is 1:20, 1:9, 1:3, 5:9, 9:5, 9:1, or 18:1;

[0016] Furthermore, the mass ratio of the compound of formula (I) to Feneptamidoquin is 1:9 to 9:1;

[0017] Furthermore, the mass ratio of the compound of formula (I) to Feneptamidoquin is 1:9, 1:3, 5:9, 9:5, or 9:1;

[0018] Furthermore, the mass ratio of the compound of formula (I) to Fenopyramid is 1:24 to 20:1;

[0019] Furthermore, the mass ratio of the compound of formula (I) to Fenopyramid is 1:24, 1:12, 1:5, 1:2, 3:1, 6:1, or 20:1;

[0020] Furthermore, the mass ratio of the compound of formula (I) to Fenopyramid is 1:12 to 3:1;

[0021] Furthermore, the mass ratio of the compound of formula (I) to Fenopyramid is 1:12, 1:5, 1:2, or 3:1;

[0022] Furthermore, based on a total weight of 100 wt% of the bactericidal composition, the sum of the contents of active ingredient A and active ingredient B in the bactericidal composition is 1 to 90 wt%.

[0023] Furthermore, based on the total weight of the bactericidal composition being 100 wt%, the sum of the contents of active ingredient A and active ingredient B in the bactericidal composition is preferably 5 to 80 wt%.

[0024] Furthermore, the bactericidal composition, in addition to containing the active ingredient, also contains agriculturally permissible auxiliary ingredients, which are selected from one or more of the following: wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists, binders, or carriers.

[0025] Further, the wetting agent is selected from one or more of alkylbenzene sulfonates, alkylnaphthalene sulfonates, lignin sulfonates, sodium dodecyl sulfate, sodium dioctyl succinate sulfonate, α-olefin sulfonates, alkylphenol polyoxyethylene ethers, castor oil polyoxyethylene ethers, alkylphenol ethoxylates, fatty alcohol ethoxylates, sodium fatty alcohol polyoxyethylene ether sulfate, silkworm excrement, soapberry powder, soapberry powder, SOPA, detergents, emulsifiers 2000 series, and wetting and penetrating agents F; and / or

[0026] Further, the dispersant is selected from one or more of the following: lignin sulfonate, alkyl naphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, tristyrylphenol ethoxylate phosphate, fatty alcohol ethoxylate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether methyl ether condensate sulfate, fatty amine polyoxyethylene ether, glycerol fatty acid ester polyoxyethylene ether, polycarboxylates, polyacrylic acids, phosphates, EO-PO block copolymers, and EO-PO graft copolymers; and / or

[0027] Further, the emulsifier is selected from one or more of the following: calcium dodecylbenzenesulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrene-phenol polyoxyethylene ether, castor oil polyoxyethylene ether, and alkylphenol ether phosphate; and / or

[0028] Furthermore, the thickener is selected from one or more of xanthan gum, organobentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose, and silica; and / or

[0029] Further, the disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid, and tartaric acid; and / or

[0030] Furthermore, the antifreeze is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons, and inorganic salts; and / or

[0031] Furthermore, the defoamer is selected from C 10 -C 20 Saturated fatty acid compounds, silicone oil, silicone compounds, C8-C 10 One or more of the fatty alcohols; and / or

[0032] Further, the solvent is selected from one or more of benzene, toluene, xylene, mesitylene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, hydrocarbon carbonates, diesel oil, solvent oil, vegetable oil (soybean oil, corn oil, rapeseed oil, palm oil, etc.), vegetable oil derivatives, and water; and / or

[0033] Further, the preservative is selected from one or more of propionic acid, sodium propionate, sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, methyl p-hydroxybenzoate, Kathon, and 1,2-benzisothiazolin-3-one; and / or

[0034] Further, the stabilizer is selected from one or more of the following: disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-methylphenol, triethanolamine oleate, epoxidized vegetable oil, kaolin, diatomaceous earth, bentonite, attapulgite, silica, talc, montmorillonite, and starch; and / or

[0035] Furthermore, the synergist is selected from synergistic phosphorus, synergistic ether; and / or

[0036] Furthermore, the carrier is selected from one or more of the following: ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils, and vegetable oil derivatives.

[0037] Furthermore, the dosage form of the bactericidal composition is selected from solid dosage forms and / or liquid dosage forms and / or seed treatment formulations;

[0038] The solid dosage forms include powders, granules, balls, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets, or soluble granules.

[0039] The liquid formulations include soluble agents, colloids, oils, spreading oils, emulsifiable concentrates, latexes, dispersible liquids, ointments, water emulsions, oil emulsions, microemulsions, lipids, suspensions, microcapsule suspensions, oil suspensions, dispersible oil suspensions, suspensions, microcapsule suspension-suspensions, microcapsule suspension-water emulsions, or microcapsule suspension-suspension emulsions.

[0040] Furthermore, the powder is a free-flowing powdered preparation containing active ingredients suitable for spraying or spreading;

[0041] Furthermore, the granules are granular preparations containing active ingredients that are free-flowing and have a certain particle size range;

[0042] Furthermore, the spherical agent is a spherical preparation containing active ingredients (generally with a diameter greater than 6 mm);

[0043] Furthermore, the tablet is a tablet-shaped preparation containing active ingredients with a certain shape and size (usually having two planes or convex surfaces, with the distance between the two surfaces being less than the diameter);

[0044] Furthermore, the strip preparation is a strip or rod-shaped preparation containing active ingredients (generally a few centimeters long and a few millimeters wide / diameter, i.e., the length is greater than the diameter / width);

[0045] Furthermore, the wettable powder is a powdered preparation in which the active ingredient is dispersed in water to form a suspension;

[0046] Furthermore, the oil-dispersible powder is a powdered preparation in which the active ingredient is dispersed in an organic solvent to form a suspension;

[0047] Furthermore, the aforementioned milk powder is a powdered preparation in which the active ingredient is dissolved in an organic solvent, encapsulated in a soluble or insoluble inert component, and dispersed in water to form an oil-in-water emulsion.

[0048] Furthermore, the water-dispersible granules are granular formulations that disintegrate in water and disperse the active ingredients into a suspension;

[0049] Furthermore, the emulsion granules are granular formulations in which the active ingredient is dissolved in an organic solvent, encapsulated in a soluble or insoluble inert component, and dispersed in water to form an oil-in-water emulsion.

[0050] Furthermore, the water-dispersible tablets are tablet formulations that disintegrate in water and disperse the active ingredients into a suspension;

[0051] Furthermore, the soluble powder is a powdered preparation in which the active ingredient forms a true solution in water, and may contain inert components that are insoluble in water;

[0052] Furthermore, the soluble granules are granular preparations in which the active ingredient forms a true solution in water, and may contain inert components that are insoluble in water;

[0053] Furthermore, the soluble tablets are tablet-shaped preparations in which the active ingredient forms a true solution in water, and may contain inert components that are insoluble in water;

[0054] Furthermore, the soluble agent is a transparent or translucent liquid preparation containing active ingredients that is diluted with water, and may contain inert components that are insoluble in water;

[0055] Furthermore, the soluble agent is a gel-like preparation containing effective ingredients that is diluted with water to form a true solution;

[0056] Furthermore, the oil is a homogeneous liquid preparation containing active ingredients, diluted (or undiluted) with an organic solvent;

[0057] Furthermore, the spreading oil is an oil containing effective ingredients that automatically diffuses into an oil film on the water surface;

[0058] Furthermore, the emulsifiable concentrate is a homogeneous liquid formulation containing active ingredients that is diluted and dispersed in water into an emulsion.

[0059] Furthermore, the latex is a latex preparation containing effective ingredients that is diluted and dispersed in water into an emulsion.

[0060] Furthermore, the dispersible liquid is a homogeneous liquid formulation containing active ingredients that is diluted and dispersed in water to form a suspension;

[0061] Furthermore, the ointment is a water-based ointment preparation containing effective ingredients that can form a film, and is generally used directly;

[0062] Furthermore, the water-in-water emulsion is a formulation in which the active ingredient (or its organic solution) forms an emulsion liquid in water;

[0063] Furthermore, the oil emulsion is a formulation in which the active ingredient (or its aqueous solution) forms an emulsion liquid in oil;

[0064] Furthermore, the microemulsion is a microemulsion liquid formulation in which the active ingredient is transparent or translucent in water, and can be used directly or after dilution with water;

[0065] Furthermore, the liposome is an oil or fat-based viscous preparation containing active ingredients, which is generally used directly;

[0066] Furthermore, the suspending agent is a stable suspension of active ingredients dispersed in water as solid particles, and is generally diluted with water before use;

[0067] Furthermore, the microcapsule suspension is a stable suspension formulation in which microcapsules containing active ingredients are dispersed in a liquid.

[0068] Furthermore, the oil suspension is a stable suspension formulation in which the active ingredient is dispersed in a liquid as solid particles, and is generally diluted with an organic solvent before use;

[0069] Furthermore, the dispersible oil suspension is a stable suspension formulation in which the active ingredient is dispersed as solid particles in a non-aqueous medium, and is generally diluted with water before use;

[0070] Furthermore, the suspension emulsion is a heterogeneous liquid formulation in which the active ingredients are stably dispersed in a continuous aqueous phase in the form of solid microparticles and water-insoluble microdroplets.

[0071] Furthermore, the microcapsule suspension-suspension agent is a stable suspension liquid formulation in which the active ingredients are dispersed in water as microcapsules and solid particles;

[0072] Furthermore, the microcapsule suspension-water emulsion is a heterogeneous liquid formulation in which the active ingredient is stably dispersed in a continuous aqueous phase in the form of microcapsules and tiny droplets.

[0073] Furthermore, the microcapsule suspension-emulsion is an active ingredient that is stably dispersed in a continuous aqueous phase in the form of microcapsules, solid particles and tiny droplets to form a heterogeneous liquid formulation.

[0074] Furthermore, the bactericidal composition can be prepared into a pesticide-acceptable formulation, wherein the formulation is a microemulsion, water-in-oil emulsion, suspension, dispersible oil suspension, soluble concentrate, emulsifiable concentrate, suspension emulsion, microcapsule suspension, water-dispersible granules, wettable powder, granules, seed treatment suspension, or seed treatment dry powder.

[0075] Furthermore, the formulation is a microemulsion, emulsifiable concentrate, suspension concentrate, water emulsion, water-dispersible granules, wettable powder, or seed treatment suspension.

[0076] The present invention also discloses the application of the bactericidal composition described above in the prevention and control of plant diseases;

[0077] Furthermore, the plant disease is a plant disease caused by fungi or bacteria;

[0078] Furthermore, the plant disease is a plant disease caused by fungi;

[0079] Furthermore, the plant disease caused by the fungus is wheat leaf rust;

[0080] Wheat leaf rust is a major disease affecting wheat growth, characterized by its wide distribution, rapid spread, and significant damage. It is a primary factor contributing to yield loss, reduced quality, and low economic returns in wheat. Wheat leaf rust is found throughout my country, and its severity is increasing year by year.

[0081] The present invention has the following beneficial effects:

[0082] 1) The bactericidal composition of the present invention increases bactericidal activity and has a synergistic effect on the target at a certain mass ratio, which can reduce the amount of pesticides used, reduce the cost of use and reduce the environmental burden.

[0083] 2) The bactericidal composition of the present invention is safe and efficient, safe for crops, non-target organisms, beneficial organisms and natural enemies, and has the effect of increasing yield and ensuring harvest. Detailed Implementation

[0084] To make the technical solution, objectives and advantages of the present invention clearer, the present invention is described with reference to the following specific embodiments. However, the present invention can be implemented in various forms and should not be limited to the embodiments described herein.

[0085] Indoor bioassay for wheat leaf rust

[0086] Experimental basis: Refer to the People's Republic of China Agricultural Industry Standard for Indoor Bioassay Test Guidelines for Pesticides, Part 15: Control of Wheat Leaf Rust Test Pot Method NY / T 1156.15-2008.

[0087] Test crop: The wheat variety Luyuan 502, which is susceptible to leaf rust, was selected and potted. 20 seeds were sown in each pot. After emergence, 10 plants were selected and numbered when they grew to the 2-leaf and 1-heart stage.

[0088] Experimental target: Puccinia recondita Rob.ex Desm.f.sp.triticiErikss.et Henn., strain obtained from Shenyang Sinochem Pesticide & Chemical R&D Co., Ltd.

[0089] Preparation of spore suspension: Fresh urediniospores of rust fungus produced within 24 hours on diseased leaves were washed off with a 0.1% Tween-80 aqueous solution and filtered through 2 to 4 layers of gauze to prepare a suspension with a concentration of 1×10⁻⁶. 5 A suspension of 1 spore / mL is prepared for later use.

[0090] Reagent preparation: Dissolve the test reagent in acetone, then dilute with 0.1% Tween 80 aqueous solution. Design different ratios according to the purpose of mixing and the activity of the reagent. Prepare the required series of mass concentrations of each group of single agents and each group of mixed agents according to the equal ratio method.

[0091] Chemical treatment: Spray the solution evenly onto the leaves until completely wet, and allow the solution to air dry naturally before use. A control group without the chemical treatment was included. Each concentration was repeated four times, with one plant per replicate.

[0092] Inoculation and culture: Inoculate with spore suspension by spray. After inoculation, wheat seedlings are cultured in the dark at 20℃ for more than 12 hours with moist conditions. The optimal temperature for the moist conditions is 15-20℃. Then, they are cultured at 18-22℃ with a light ratio of L:D = 12:12h.

[0093] Data survey: When the incidence rate in the blank control reaches more than 80%, the incidence rate of each treatment will be investigated in a graded manner.

[0094] The grading method is as follows:

[0095] Level 0: No spore-bearing mass;

[0096] Grade 1: Spore accumulation accounts for less than 5% of the total leaf area;

[0097] Level 3: The spore mass accounts for 5% to 10% of the total leaf area;

[0098] Level 5: The spore mass accounts for 10% to 25% of the total leaf area;

[0099] Level 7: The spore mass accounts for 25% to 50% of the entire leaf area;

[0100] Level 9: The spore mass accounts for more than 50% of the total leaf area.

[0101] Data statistics and analysis:

[0102] The disease index is calculated using the following formula.

[0103]

[0104] In the formula:

[0105] X—Disease index;

[0106] N i —Number of diseased leaves at each level;

[0107] i — relative numerical value;

[0108] N—Total number of leaves surveyed.

[0109] The prevention and control effect is calculated using the following formula.

[0110]

[0111] In the formula:

[0112] P – Prevention and control effect, in percentage (%);

[0113] CK – Blank control disease index;

[0114] PT – Disease index dictated by medication treatment.

[0115] According to the standard method of bioassay, the synergistic effect of the mixture of agents was evaluated according to the co-toxicity coefficient method (CTC) of Sun Yunpei, that is, CTC ≤ 80 is antagonistic effect, 80 < CTC < 120 is additive effect, and CTC ≥ 120 is synergistic effect.

[0116] The co-toxicity coefficient (CTC) is calculated according to the following formula.

[0117]

[0118] In the formula:

[0119] ATI—the measured toxicity index of the mixture;

[0120] S—the EC of the standard agent 50 , with the unit of milligram per liter (mg / L);

[0121] M—the EC of the mixture 50 , with the unit of milligram per liter (mg / L).

[0122] TTI = TI A ×P A +TI B ×P B

[0123] In the formula:

[0124] TTI—the theoretical toxicity index of the mixture;

[0125] TI A —the toxicity index of agent A;

[0126] P A —the percentage content of agent A in the mixture, with the unit of percentage (%);

[0127] TI B —the toxicity index of agent B;

[0128] P B —the percentage content of agent B in the mixture, with the unit of percentage (%).

[0129]

[0130] In the formula:

[0131] CTC—the co-toxicity coefficient;

[0132] ATI—the measured toxicity index of the mixture;

[0133] TTI—the theoretical toxicity index of the mixture.

[0134] The experimental results were calculated using DPS data processing software, and the toxicity regression equations and EC values ​​for single-dose and mixtures with different ratios of the test drug were derived. 50 And 95% confidence limit.

[0135] Results of indoor bioactivity assay:

[0136] Table 1 shows that compound (I) controls wheat leaf rust EC. 50 The concentration of feneptamidoquin was 0.2407 mg / L, which is effective in controlling wheat leaf rust EC. 50 The concentration was 0.4519 mg / L. Compound (I) exhibited a synergistic effect when mixed with feneptamidoquin at a mass ratio of 1:20 to 18:1. Among these, the co-toxicity coefficient was >150 at mass ratios of 1:20 to 9:1, indicating a significant synergistic effect. The co-toxicity coefficient of compound (I) with feneptamidoquin at a ratio of 5:9 was the highest, at 296.362, EC50. 50 It is 0.1161 mg / L.

[0137] Table 1 shows the synergistic effect of compound (I) with feneptamidoquin in different ratios on wheat leaf rust.

[0138]

[0139] Table 2 shows that fenopyramid effectively controls wheat leaf rust EC. 50 The concentration was 0.6061 mg / L. When the mass ratio of compound (I) to fenopyramid was 1:36–20:1, the co-toxicity coefficient was >80, indicating an additive or synergistic effect; when the mass ratio was 1:24–20:1, the co-toxicity coefficient was >120, indicating a synergistic effect. The co-toxicity coefficient of compound (I) with fenopyramid at a ratio of 1:2 was the highest, at 212.040, EC50. 50 The concentration was 0.1905 mg / L, indicating a significant synergistic effect.

[0140] Table 2 shows the results of the synergistic effect of different ratios of compound (I) with fenopyramid on wheat leaf rust.

[0141]

[0142]

[0143] Formulation Examples

[0144] Preparation Example 1:

[0145] 42% Formula (I) compound Feneptamidoquin wettable powder (15:27)

[0146] Formulation: 15% of compound (I), 27% of Feneptamidoquin, 5% of sodium polycarboxylate, 5% of dispersant NNO, 5% of polyoxyethylene ether (NV1420), 3.5% of sodium dodecyl sulfate, 5% of bentonite, and the balance made up with kaolin.

[0147] Preparation method: The active ingredients, other functional additives and fillers are mixed according to the formula ratio, stirred evenly in a stirring tank, and then pulverized and mixed evenly multiple times by an air jet mill to prepare the wettable powder of the composition of the present invention.

[0148] Preparation Example 2:

[0149] 22% Formula (I) Compound Feneptamidoquin Suspension (8:14)

[0150] Formulation: 8% of compound (I), 14% of Feneptamidoquin, 4% of polyether, 3% of phenethylphenol polyether phosphate salt, 3% of calcium sulfonyl succinate (EP60P), 1.5% of sodium polycarboxylate, 1% of magnesium aluminum silicate, 0.25% of xanthan gum, 4.5% of ethylene glycol, 0.02% of benzisothiazolinone, 0.5% of organosilicon defoamer, and deionized water to make up the balance;

[0151] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in the reaction vessel in sequence, water is added and mixed evenly, and then subjected to high-speed shearing, wet sand milling and finally homogenization filtration to obtain the suspension.

[0152] Preparation Example 3:

[0153] 12% Formula (I) Compound Feneptamidoquin Emulsifiable Concentrate (2:10)

[0154] Preparation formula: 2% of compound (Ⅰ), 10% of Feneptamidoquin, 1.5% of BHT, 10% of propylene carbonate, 15% of DMF, 5% of cyclohexanone, 3.5% of calcium dodecylbenzenesulfonate, 10% of fatty alcohol polyoxyethylene ether, and methyl oleate to make up the balance;

[0155] Preparation method: Add the active ingredient to the cosolvent according to the formulation ratio of the example, and add surfactants and other functional additives thereto. Stir and mix evenly in a stirring mixing tank to obtain emulsifiable oil.

[0156] Preparation Example 4:

[0157] 24% Formula (I) Compound·Fenopyramid Suspension (8:16)

[0158] Formulation: 8% of compound (Ⅰ), 16% of Fenopyramid, 4% of polyether, 3% of phenethylphenol polyether phosphate salt, 3% of sodium dioctyl succinate sulfonate, 1.5% of sodium polycarboxylate, 1% of magnesium aluminum silicate, 0.2% of xanthan gum, 4.5% of ethylene glycol, 0.02% of benzisothiazolinone, 0.5% of organosilicon defoamer, and deionized water to make up the balance;

[0159] Preparation method: Same as in preparation example 2.

[0160] Preparation Example 5:

[0161] 48% Formula (I) Compound·Fenopyramid Water Dispersible Granules (16:32)

[0162] Formulation: 16% of compound (I), 32% of Fenopyramid, 3.5% of sodium dodecyl sulfate, 8% of polynaphthalene sulfonate (270K), 1% of sodium fatty alcohol polyoxyethylene ether sulfate, 2% of sodium polycarboxylate BX, 6.5% of sodium lignin sulfonate, 7% of attapulgite, and the balance is made up of kaolin.

[0163] Preparation method: According to the formula ratio, add the active ingredients to the carrier, and add surfactants and other functional additives to it. Mix, and after air jet milling, add 10-25% water. Then knead, granulate, dry and sieve to obtain water-dispersible granules; or spray water, granulate and dry the pulverized powder in a fluidized bed granulator, and then sieve to obtain the product.

[0164] Field efficacy trial of wheat leaf rust

[0165] Experimental basis: Refer to "Guidelines for Field Efficacy Tests of Pesticides (I) Control of Cereal Rusts (Leaf Rust, Stripe Rust, Stem Rust) with Fungicides (GB / T 17980.23-2000)";

[0166] Experiment location: Wheat field in Nanfan Village, Shouguang City, Shandong Province;

[0167] Experimental target: Puccinia recondita f.sp.tritici., the fungus causing wheat leaf rust;

[0168] Experimental crop and variety: Wheat (Tanmai 98);

[0169] Experimental setup: Each cell was randomly distributed across multiple blocks, with a guard row surrounding each cell. Each treatment was replicated four times, with each cell measuring 20m. 2 The cells are randomly arranged.

[0170] Test reagents:

[0171] Table 3. Experimental treatments and drug dosages

[0172] medicine <![CDATA[Dosage g a.i / hm 2 > 22% Formula (I) Compound Feneptamidoquin Suspension (8:14) 75 24% Formula (I) Compound·Fenopyramid Suspension (8:16) 75 30% Formula (I) Compound Suspension 100 15% Feneptamidoquin suspension 200 20% Fenopyramid suspension 250 Water comparison /

[0173] Experimental methods: Conventional spraying was used to apply the pesticide at the early stage of wheat leaf rust (wheat grain-filling stage), followed by a second application 7 days later, for a total of two applications. The pesticide was sprayed evenly on both sides of the leaves. The field water volume was 600 L / hm². 2 .

[0174] Survey and statistical methods: The survey was conducted 10 days after the last application of the pesticide. Five points were randomly selected from the diagonal of each plot, and 20 plants were surveyed at each point. The top 3 leaves of each plant were surveyed (including the flag leaf if present). The plants were graded according to the percentage of disease spots on each leaf covering the entire leaf area.

[0175] Grading method:

[0176] Level 0: No disease;

[0177] Grade 1: The area of ​​lesions accounts for less than 5% of the total leaf area;

[0178] Grade 3: The area of ​​lesions accounts for 6% to 25% of the total leaf area;

[0179] Level 5: The area of ​​lesions accounts for 26% to 50% of the total leaf area;

[0180] Level 7: The area of ​​lesions accounts for 51% to 75% of the total leaf area;

[0181] Level 9: The lesion area accounts for more than 75% of the total leaf area.

[0182] Methods for calculating drug efficacy

[0183] Calculate the disease index and prevention efficacy using the following formula.

[0184]

[0185] During the experiment, wheat growth was observed to be good in all treatment plots, and no herbicide damage was observed in any treatment.

[0186] Wheat yield was measured in plots at the wheat harvest period.

[0187] Experimental results:

[0188] As shown in Table 4, the combination of compound (I) with Feneptamidoquin and Fenopyramid showed good control effects against wheat leaf rust. Ten days after the last application, the control efficacy of 22% compound (I)·Feneptamidoquin suspension (8:14) and 24% compound (I)·Fenopyramid suspension (8:16) against wheat leaf rust was 83.10% and 85.13%, respectively, showing excellent control effects that were significantly higher than the single-agent control.

[0189] Table 4. Results of field efficacy trials of different treatments for controlling wheat leaf rust.

[0190]

[0191]

[0192] Note: The disease index and prevention efficacy in the table are the average values ​​of each replicate; different lowercase letters in the same column indicate statistically significant differences between groups (p < 0.05).

[0193] As shown in Table 5, 22% of Formula (I) compound Feneptamidoquin suspension (8:14), 24% of Formula (I) compound

[0194] Fenopyramid suspension (8:16) showed a significant yield-increasing effect on wheat, with yield increases of 18.95% and 16.72%, respectively.

[0195] Table 5. Effects of different treatments on wheat yield

[0196]

[0197] No phytotoxicity or impact on crop growth and development was recorded during the experiment, indicating that the tested pesticide was safe for crops within the recommended dosage range. No effects on non-target organisms were found within the dosage range. No extreme weather events such as heavy rainfall or strong winds were recorded during the experiment.

[0198] Field efficacy trials showed that the compound shown in formula (I), in combination with Feneptamidoquin and Fenopyramid, exhibited high control efficacy in wheat leaf rust control. It not only reduced the disease index and demonstrated significant control effects, but also had little impact on yield. Furthermore, the mixed formulation showed a significant yield-increasing effect. Post-treatment observations indicated that wheat growth in all treatment areas was normal, with no phytotoxicity observed, demonstrating the safety of each agent at the experimental dosages.

[0199] Through indoor toxicity testing and field trials, the compound shown in formula (I) of this invention, combined with either Feneptamidoquin or Fenopyramid, exhibited excellent control effects against wheat leaf rust, demonstrating broad application prospects. The fungicidal composition or formulation obtained by this invention exhibits significant efficacy, characterized by high efficiency, broad spectrum, low residue, long-lasting effect, and strong systemic activity. Furthermore, no phytotoxicity was observed in the experiments, indicating that the enhanced synergistic effect of the obtained fungicidal composition or formulation reduces production and usage costs while ensuring crop safety.

[0200] Although the present invention has been described in detail above with general description and specific embodiments, some modifications or improvements can be made to it based on the present invention, which will be obvious to those skilled in the art. Therefore, all such modifications or improvements made without departing from the spirit of the present invention are within the scope of protection claimed by the present invention.

Claims

1. A bactericidal composition, characterized in that: It comprises active ingredient A and active ingredient B, wherein active ingredient A is a compound of formula (I), with the following chemical structural formula: (I), Active ingredient B is either Feneptamidoquin or Fenopyramid; the mass ratio of the compound of formula (I) to Feneptamidoquin is 1:20 to 18:1; the mass ratio of the compound of formula (I) to Fenopyramid is 1:24 to 20:

1.

2. The bactericidal composition according to claim 1, characterized in that, The mass ratio of the compound of formula (I) to Feneptamidoquin is 1:20, 1:9, 1:3, 5:9, 9:5, 9:1, or 18:1; the mass ratio of the compound of formula (I) to Fenopyramid is 1:24, 1:12, 1:5, 1:2, 3:1, 6:1, or 20:

1.

3. The bactericidal composition according to claim 1, characterized in that, Based on a total weight of 100 wt% of the bactericidal composition, the sum of the contents of active ingredient A and active ingredient B in the bactericidal composition is 1 to 90 wt%.

4. The bactericidal composition according to claim 1, characterized in that, Based on a total weight of 100 wt% of the bactericidal composition, the sum of the contents of active ingredient A and active ingredient B in the bactericidal composition is 5-80 wt%.

5. The bactericidal composition according to claim 1, characterized in that, In addition to the active ingredient, the bactericidal composition contains agriculturally permissible auxiliary ingredients, which are selected from one or more of the following: wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists, binders, or carriers.

6. The bactericidal composition according to claim 5, characterized in that, The formulation of the bactericidal composition is selected from microemulsions, emulsifiable concentrates, suspensions, water emulsions, water-dispersible granules, wettable powders, and seed-treated suspensions.

7. The application of the bactericidal composition according to any one of claims 1-6 in the prevention and control of plant diseases, characterized in that, The plant disease mentioned is wheat leaf rust caused by fungi.

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