Production process using organic stevioside as table sugar substitute
By improving the stevia production process, using ultrasonic and microwave extraction technology, combining ethanol configuration and erythritol mixed granulation, the problems of low extraction rate, insufficient purity and high production costs in the traditional stevia production process were solved, and high quality table sugar substitutes were prepared, with the characteristics of low calories, good taste and high solubility.
Patent Information
- Application Number
- CN202411527052.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-30
- Publication Date
- 2025-03-18
AI Technical Summary
The traditional stevia production process has problems such as low extraction rate, insufficient purity and high production costs, which limits its large-scale application.
A process is adopted, including crushing fresh stevia leaves with purified water and complex enzyme a, extracting and filtration through ultrasonic extraction and filtration, then mixing the slag with purified water and complex enzyme b, microwave extraction and filtration, combining and concentrated and dried to obtain organic stevia, then adding ethanol to arrange it into a slurry, and mixing it with erythritol to granulate to obtain a table sugar substitute product.
The table sugar substitute products obtained through this process have a good taste, low calories, high solubility, and uniform color. They can replace traditional sweeteners such as sucrose and are suitable for a wide range of applications in the food field.
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Abstract
Description
Technical Field
[0001] The invention relates to the technical field of stevioside processing, and in particular to a production process using organic stevioside as a table sugar substitute. Background Art
[0002] Traditional sugars such as sucrose, although sweet, are also relatively high in calories. Long-term excessive intake may increase health risks such as obesity and diabetes. Therefore, finding a natural sugar substitute that can meet the sweetness needs and has low-calorie characteristics has become an important research direction in the food industry. Organic stevia, a natural sweetener extracted from stevia plants, has gradually gained market favor due to its high sweetness (up to 200 to 350 times that of sucrose), low calories (only 1 / 300 of sucrose), and good stability and safety. However, the traditional stevia production process has problems such as low extraction rate, substandard purity, and high production cost, which limits its large-scale application. Therefore, in response to the above problems, it is necessary to develop a production process using organic stevia as a table sugar substitute. Summary of the invention
[0003] The technical problem to be solved by the present invention is: in view of the deficiencies of the prior art, a production process using organic stevia as a table sugar substitute is provided, by which a table sugar substitute product with good taste, low calories, good solubility and uniform color can be obtained.
[0004] In order to solve the above technical problems, the technical solution of the present invention is:
[0005] A production process using organic stevia as a table sugar substitute comprises the following steps:
[0006] (1) Preparation of organic stevioside:
[0007] S1, take fresh stevia leaves after impurities are removed, add purified water and compound enzyme a to the crushed and sieved stevia powder, adjust the pH, perform ultrasonic extraction, filter, and collect the extract and residue for later use;
[0008] S2, taking the residue described in S1, adding purified water and complex enzyme b, adjusting the pH, performing microwave extraction, filtering, and collecting the extract for later use;
[0009] S3, combining the extract in S1 and the extract in S2, concentrating and drying to obtain organic stevioside;
[0010] (2) Preparation of organic stevia slurry: adding the organic stevia described in step (1) to a solvent and stirring to obtain an organic stevia slurry;
[0011] (3) Compounding and granulation: Take erythritol, add the organic stevia syrup described in step (2), mix and granulate to obtain a table sugar substitute product.
[0012] As an improved technical solution, in step (1), the pH is adjusted to 6-6.8 in S1, and the amount of complex enzyme a is 0.2-0.5% of the weight of the stevia powder. The complex enzyme a includes cellulase, pectinase and hemicellulase, and the cellulase, pectinase and hemicellulase are mixed in a mass ratio of 1-3:1-2:1-2.
[0013] As an improved technical solution, the power of ultrasonic extraction in S1 of step (1) is 550-650w, the extraction temperature is 55-65°C, and the ultrasonic extraction time is 10-15min.
[0014] As an improved technical solution, the pH in S2 of step (1) is adjusted to 5-6.5, and the dosage of the complex enzyme b is 0.2-0.8% of the weight of the slag, and the complex enzyme b includes protease, ligninase and amylase, and the protease, ligninase and cellulase are mixed in a mass ratio of 1-2:1-1.5:1-2.
[0015] As an improved technical solution, the power of microwave extraction in S2 of step (1) is 300-400w, the extraction temperature is 40-60°C, and the extraction time is 15-30min.
[0016] As an improved technical solution, the solvent in step (2) is ethanol with a volume concentration of 70-75%, and the organic stevioside and the ethanol are mixed in a solid-liquid ratio of 1:3-8.
[0017] As an improved technical solution, in step (2), the erythritol and the organic stevia slurry are mixed in a solid-liquid ratio of 5:1-2.
[0018] After adopting the above technical solution, the beneficial effects of the present invention are:
[0019] The present invention crushes and sieves fresh stevia leaves after impurities removal, adds purified water and compound enzyme a, performs ultrasonic extraction and filtering, collects filtrate containing components such as steviol glycosides, chlorophyll, chlorogenic acid, etc.; adds purified water and compound enzyme b to the residue, performs microwave extraction and filtering, collects filtrate containing components such as steviol glycosides, small molecule proteins, small molecule polysaccharides, etc.; then combines, concentrates, and dries the collected filtrates to obtain organic steviol glycoside powder containing multiple active ingredients, then adds ethanol to prepare organic steviol glycoside slurry, and finally sprays it on the surface of erythritol, mixes, dries, and granulates to obtain a table sugar substitute product. The above process method prepares a table sugar product containing multiple active ingredients, low in calories, and good in taste, which can replace common sweeteners such as sucrose on the market and can be widely used in the field of food. DETAILED DESCRIPTION
[0020] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.
[0021] Example 1
[0022] A production process using organic stevia as a table sugar substitute comprises the following steps:
[0023] (1) Preparation of organic stevia:
[0024] S1, take fresh stevia leaves after impurities are removed, crush and sieve stevia powder 100g, add 900mL of purified water and compound enzyme a (the dosage of compound enzyme a is 0.2% of the weight of stevia powder, and cellulase, pectinase and hemicellulase are mixed in a mass ratio of 1:1:1), adjust the pH to 6, perform ultrasonic extraction (extraction power is 550w, extraction temperature is 55°C, ultrasonic extraction time is 10min), plate and frame filtration, collect 820mL of extract and 200g of residue respectively for standby use;
[0025] S2, take 200g of the residue in S1, add 600mL of purified water and complex enzyme b (the dosage of complex enzyme b is 0.2% of the weight of the residue, and protease, ligninase and cellulase are mixed in a mass ratio of 1:1:1), adjust the pH to 5, microwave extraction (extraction power is 300w, extraction temperature is 40°C, extraction time is 15min), ultrafiltration membrane filtration, and collect 530mL of extract for standby use;
[0026] S3, combining the extract in S1 and the extract in S2, heat concentrating and drying to obtain 45 g of organic stevioside;
[0027] (2) Preparation of organic stevia slurry: 10 g of the organic stevia prepared in step (1) was added with 30 mL of 70% ethanol (the organic stevia and ethanol were mixed in a solid-liquid ratio of 1:3) and stirred to obtain 35 mL of organic stevia slurry with a solid content of 27 wt %;
[0028] (3) Compounding and granulation: Take 50 g of erythritol, add 10 mL of the organic stevia syrup in step (2) (erythritol and organic stevia syrup are mixed in a solid-liquid ratio of 5:1), granulate, and dry to obtain a table sugar substitute product.
[0029] Example 2
[0030] A production process using organic stevia as a table sugar substitute comprises the following steps:
[0031] (1) Preparation of organic stevia:
[0032] S1, take fresh stevia leaves after impurities are removed, crush and sieve stevia powder 100g, add 800mL of purified water and compound enzyme a (the dosage of compound enzyme a is 0.35% of the weight of stevia powder, and cellulase, pectinase and hemicellulase are mixed in a mass ratio of 2:1.5:1.5), adjust the pH to 6.5, and perform ultrasonic extraction (extraction power is 600w, extraction temperature is 60°C, and ultrasonic extraction time is 12min), plate and frame filtration, and collect 730mL of extract and 180g of residue for standby use;
[0033] S2, take 180g of the residue in S1, add 700mL of purified water and complex enzyme b (the dosage of complex enzyme b is 0.5% of the weight of the residue, and the protease, ligninase and cellulase are mixed in a mass ratio of 1.2:1.2:1.5), adjust the pH to 5.8, microwave extraction (extraction power is 350w, extraction temperature is 50°C, extraction time is 23min), ultrafiltration membrane filtration, and collect 640mL of the extract for standby use;
[0034] S3, combining the extract in S1 and the extract in S2, heat concentrating and drying to obtain 48 g of organic stevioside;
[0035] (2) Preparation of organic stevia slurry: 10 g of the organic stevia prepared in step (1) was added with 50 mL of 72% ethanol (the organic stevia and ethanol were mixed in a solid-liquid ratio of 1:5) and stirred to obtain 56 mL of organic stevia slurry with a solid content of 17.8 wt %;
[0036] (3) Compounding and granulation: Take 25 g of erythritol, add 7.5 mL of the organic stevia syrup in step (2) (erythritol and organic stevia syrup are mixed in a ratio of 5:1.5), granulate and dry to obtain a table sugar substitute product.
[0037] Example 3
[0038] A production process using organic stevia as a table sugar substitute comprises the following steps:
[0039] (1) Preparation of organic stevioside:
[0040] S1, take fresh stevia leaves after impurities are removed, crush and sieve stevia powder 200g, add 1800mL of purified water and compound enzyme a (the dosage of compound enzyme a is 0.5% of the weight of stevia powder, and cellulase, pectinase and hemicellulase are mixed in a mass ratio of 3:2:2), adjust the pH to 6.8, and perform ultrasonic extraction (extraction power is 650w, extraction temperature is 65°C, and ultrasonic extraction time is 15min), plate and frame filtration, and collect 1650mL of extract and 380g of residue for standby use;
[0041] S2, take 380g of the residue in S1, add 1200mL of purified water and compound enzyme b (the dosage of compound enzyme b is 0.8% of the weight of the residue, and protease, ligninase and cellulase are mixed in a mass ratio of 2:1.5:2), adjust the pH to 6.5, perform microwave extraction (extraction power is 400w, extraction temperature is 60°C, extraction time is 30min), filter with ultrafiltration membrane, and collect 1000mL of extract for standby use;
[0042] S3, combining the extract in S1 and the extract in S2, heat concentrating and drying to obtain 92 g of organic stevioside;
[0043] (2) Preparation of organic stevia slurry: 20 g of the organic stevia prepared in step (1) was added with 160 mL of 75% ethanol (the organic stevia and ethanol were mixed in a solid-liquid ratio of 1:8) and stirred to obtain 178 mL of organic stevia slurry having a solid content of 11.2 wt %;
[0044] (3) Compounding and granulation: Take 50 g of erythritol, add 20 mL of the organic stevia syrup in step (2) (erythritol and organic stevia syrup are mixed in a solid-liquid ratio of 5:2), mix, granulate and dry to obtain a table sugar substitute product.
[0045] In order to better prove that the production process of this method can obtain table sugar substitute products with better indicators, 6 comparative examples are given with reference to Example 2, and the details are shown in the following content.
[0046] Comparative Example 1
[0047] The difference from Example 2 is that no ligninase is added in S1 of step (1), and the rest of the operations are the same.
[0048] Comparative Example 2
[0049] The difference from Example 2 is that in step (1) S1, the pH is adjusted to 5, and the remaining operations are the same.
[0050] Comparative Example 3
[0051] The difference from Example 2 is that the extraction temperature in S1 of step (1) is 50° C., and the rest of the operations are the same.
[0052] Comparative Example 4
[0053] The difference from Example 2 is that the power extracted in S1 of step (1) is 500w, and the rest of the operations are the same.
[0054] Comparative Example 5
[0055] The difference from Example 2 is that no protease is added in S2 of step (1), and the rest of the operations are the same.
[0056] Comparative Example 6
[0057] The difference from Example 2 is that in step (1) S2, the pH is adjusted to 4.5, and the remaining operations are the same.
[0058] Comparative Example 7
[0059] The difference from Example 2 is that the extraction temperature in S2 of step (1) is 55° C., and the remaining operations are the same.
[0060] Comparative Example 8
[0061] The difference from Example 2 is that the power extracted in S1 of step (1) is 450W, and the rest of the operations are the same.
[0062] Table 1
[0063]
[0064]
[0065] It can be found from the data in Table 1 that the table sugar substitute product prepared by the production process Example 2 of the present invention has significantly better indicators than those of the other examples and comparative examples 1-8.
[0066] The solubility test of the table sugar substitutes prepared in Examples 1-3 and Comparative Examples 1-8 is detailed in the following.
[0067] Weigh 5 g of each product in Examples 1-3 and Comparative Examples 1-8, where both Examples 1 and 3 are 20-mesh particles, and both Examples 2 and Comparative Examples 1-8 are 40-mesh particles, and put them into 100 ml of 25°C water, respectively, and observe the time taken for complete dissolution under stirring (45 rpm) and without stirring, as shown in Table 2 for details; weigh 5 g of each product in Examples 1-3 and Comparative Examples 1-8, respectively, and put them into 100 ml of 60°C water, and observe the time taken for complete dissolution under stirring (45 rpm) and without stirring, as shown in Table 3 for details.
[0068] Table 2
[0069]
[0070]
[0071] Table 3
[0072] Under stirring conditions of 45 rpm Without stirring Example 1 20s 45s Example 2 12s 32s Example 3 22s 47s Comparative Example 1 19s 38s Comparative Example 2 21s 40s Comparative Example 3 22s 42s Comparative Example 4 25s 45s Comparative Example 5 23s 46s Comparative Example 6 18s 43s Comparative Example 7 17s 42s Comparative Example 8 20s 45s
[0073] It can be found from Table 2 and Table 3 that the solubility of the table sugar substitute product in Example 2 is significantly better than that in other Examples and Comparative Examples 1-8.
[0074] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.
Claims
1. A production process using organic stevia as a table sugar substitute, characterized in that: The following steps are involved: (1) Preparation of organic stevia: S1, take fresh stevia leaves after impurities are removed, add purified water and compound enzyme a to the crushed and sieved stevia powder, adjust the pH, perform ultrasonic extraction, filter, and collect the extract and residue for later use; S2, taking the residue described in S1, adding purified water and complex enzyme b, adjusting the pH, performing microwave extraction, filtering, and collecting the extract for later use; S3, combining the extract in S1 and the extract in S2, concentrating and drying to obtain organic stevioside; (2) Preparation of organic stevia slurry: adding the organic stevia described in step (1) to a solvent and stirring to obtain an organic stevia slurry; (3) Compounding and granulation: Take erythritol, add the organic stevia syrup described in step (2), mix and granulate to obtain a table sugar substitute product.
2. The production process of using organic stevia as a table sugar substitute according to claim 1, characterized in that: In step (1), the pH is adjusted to 6-6.8 in S1, and the amount of compound enzyme a is 0.2-0.5% of the weight of the stevia powder. The compound enzyme a includes cellulase, pectinase and hemicellulase, and the cellulase, pectinase and hemicellulase are mixed in a mass ratio of 1-3:1-2:1-2.
3. The production process of using organic stevia as a table sugar substitute according to claim 1, characterized in that: In step (1), the power of ultrasonic extraction in S1 is 550-650w, the extraction temperature is 55-65°C, and the ultrasonic extraction time is 10-15min.
4. The production process of using organic stevia as a table sugar substitute according to claim 1, characterized in that: In step (1), the pH is adjusted to 5-6.5 in S2, and the dosage of the complex enzyme b is 0.2-0.8% of the weight of the slag. The complex enzyme b includes protease, ligninase and amylase, and the protease, ligninase and cellulase are mixed in a mass ratio of 1-2:1-1.5:1-2.
5. The production process of using organic stevia as table sugar substitute according to claim 1, characterized in that: In step (1) S2, the power of microwave extraction is 300-400w, the extraction temperature is 40-60°C, and the extraction time is 15-30min.
6. The production process of using organic stevia as a table sugar substitute according to claim 1, characterized in that: In step (2), the solvent is ethanol with a volume concentration of 70-75%, and the organic stevioside and the ethanol are mixed in a solid-liquid ratio of 1:3-8.
7. The production process of using organic stevia as a table sugar substitute according to claim 1, characterized in that: In step (2), the erythritol and the organic stevia slurry are mixed in a solid-liquid ratio of 5:1-2.