Edible fungus liquid strain multi-tank breeding system
By designing a multi-can tank breeding system for liquid edible fungi, the problem that traditional devices cannot meet the large-scale production of edible fungi is solved, and large-scale strain cultivation and efficient and clean strain production are achieved.
Patent Information
- Application Number
- CN202411810108.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-10
- Publication Date
- 2025-03-21
AI Technical Summary
Traditional single-can strain cultivation devices cannot meet the needs of large-scale and industrial production of edible fungi, and there is a problem of inconsistent strain age during the large-scale breeding of liquid strains.
A multi-connection tank breeding system for edible fungi liquid strains was designed, and large-scale strain cultivation was realized through multiple strain cultivation devices connected in sequence. The system includes a cultivation tank, a cultivation environment control device, a cultivation temperature control device and a feeding device, and has the characteristics of efficient strain transportation, precise environmental and temperature control, automation and intelligent operation.
Large-scale cultivation of bacterial strains has been achieved, the problem that traditional devices cannot meet large-scale production is solved, the efficiency and success rate of bacterial strain cultivation is improved, and the cleanliness and consistency of bacterial strains is ensured.
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Figure CN119655114A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the technical field of edible fungus liquid strain breeding, and more specifically to an edible fungus liquid strain multi-tank breeding device. Background Art
[0002] The cultivation of strains adopts aseptic operation methods to separate edible fungi from mixed microorganisms. This process is called strain separation. The mycelium obtained from the separation process is purified to become a pure strain. The method of artificially growing and reproducing pure strains in large quantities under laboratory conditions is called strain cultivation.
[0003] In the production of edible fungi, liquid strains are being adopted by more and more companies due to their advantages such as short culture cycle, low cost and strong strain vitality. However, the current liquid strain production equipment and production process have problems such as small culture containers, limited one-time fermentation volume, and difficulty in supporting the factory-based and large-scale production of the edible fungi industry. With the increasing scale of edible fungi production, this contradiction between supply and demand has become more and more prominent and has become a bottleneck problem in the factory-based production of edible fungi.
[0004] The patent with publication number CN109328883B discloses a culture device for edible fungi strains and a method for cultivating edible fungi strains, including an open box, in which a first culture chamber and a second culture chamber are formed by a partition, the first culture chamber is provided with a culture medium sterilization device and a strain culture device, the second culture chamber is provided with an air purification device, and the entire culture box is provided with a culture medium sterilization device and a strain culture device, which can transfer the sterilized culture medium to the strain culture device in time for cultivating edible fungi strains, thereby avoiding secondary contamination of the culture medium affecting the cultivation of edible fungi strains.
[0005] The patent with publication number CN109429912A discloses a liquid spawn cultivation device for Pleurotus eryngii, including a shell, a resistance heating plate and a semiconductor resistor plate are installed on the inner back side of the shell, shafts are rotatably installed on the left and right sides of the inside of the shell, the shafts on the right side penetrate the shell and are connected to pulleys, a swing device is installed on the lower right side of the shell, one end of the second water supply pipe penetrates the shell and the water storage frame, fixed blocks are symmetrically installed on both sides of the inside of the shell, a guide shaft is fixedly installed between the two corresponding fixed blocks, a cultivation device is installed between the two corresponding sliders, and an air supply device is installed on the left side of the outside of the shell.
[0006] However, the traditional single-tank strain cultivation device cannot carry out large-scale strain cultivation due to its long breeding cycle and limited capacity, and cannot meet the needs of large-scale and industrialized development of edible fungi. When large-scale strain cultivation is required, it can only be achieved by increasing the number of tanks. Under suitable conditions, liquid strains can reproduce very quickly. However, taking shiitake mushrooms as an example, the breeding cycle of liquid strains in a 600L single-tank environment requires 8-12 days. Therefore, single-tank breeding of liquid strains has the common industry pain point of inconsistent strain age. If liquid strains are cultivated in part, they are taken out manually and placed in a larger strain cultivation device for cultivation. During the transfer process, the breeding environment will change and the strains cannot be controlled from being contaminated.
[0007] Therefore, how to provide a large-tonnage multi-tank breeding device for liquid edible fungi strains, meet the current large-scale and industrialized production of edible fungi through the combined cultivation of large-tonnage multi-tanks, and enable the strains to automatically flow to the next cultivation environment after cultivation is completed is a problem that technical personnel in this field urgently need to solve. Summary of the invention
[0008] In view of this, the present invention provides a multi-tank breeding system for liquid edible fungi, aiming to solve the technical problem that large-scale cultivation of edible fungi cannot be carried out.
[0009] In order to achieve the above object, the present invention adopts the following technical solution:
[0010] An edible fungus liquid strain multi-tank breeding system, comprising: a plurality of strain cultivation devices connected in sequence;
[0011] The bacterial strain cultivation device comprises a cultivation tank, a cultivation environment control device, a cultivation temperature control device and a feeding device. The feeding device is fixedly mounted on the top of the cultivation tank and is used to feed raw materials. The cultivation environment control device is connected to the inside of the cultivation tank and is used to regulate the cultivation environment inside the cultivation tank. The cultivation temperature control device is connected to the hollow cavity wall of the cultivation tank and is used to control the cultivation temperature inside the cultivation tank through high-temperature gas or low-temperature liquid.
[0012] A seed transfer device is arranged at the bottom of the strain culture device, and the strains are transported to the connected strain culture device through the seed transfer device.
[0013] Furthermore, the cultivation environment control device includes an input device and an exhaust device, the input device includes a first high-temperature steam input pipeline and a high-pressure clean gas input pipeline, and the first high-pressure steam input pipeline and the high-pressure clean gas input pipeline are connected to the cultivation tank body through valves.
[0014] Furthermore, a steam main valve and a steam valve are provided on the first high-temperature steam input pipeline, and a steam exhaust valve is also provided between the steam main valve and the steam valve.
[0015] Furthermore, the high-pressure clean gas input pipeline includes a high-pressure air input pipeline and a second high-temperature steam input pipeline. A sterilization device is provided on the high-pressure air input pipeline. The second high-temperature steam input pipeline is connected to the sterilization device and is used to sterilize the sterilization device by high-temperature steam. The sterilization device is used to sterilize the high-pressure air in the high-pressure air input pipeline. An air main valve, a clean air valve and an air filter exhaust valve are provided on the high-pressure air input pipeline. A plurality of air filter steam valves are provided on the second high-pressure steam input pipeline.
[0016] Furthermore, the sterilization device is also provided with a pressure detection device and a drain valve;
[0017] Furthermore, the discharge device includes a discharge pipeline and a pressure detection device, an air outlet main valve, an air flow meter and a drain valve which are sequentially arranged on the discharge pipeline, and the air flow meter is also provided with a drain valve.
[0018] Furthermore, the cultivation temperature control device specifically includes an interlayer steam inlet pipeline, an interlayer water circulation outlet pipeline, an interlayer steam outlet pipeline and an interlayer water circulation inlet pipeline;
[0019] A steam valve is provided on the interlayer steam inlet pipeline, a drain valve is provided on the interlayer water circulation outlet pipeline, the drain valve is provided on the interlayer steam outlet pipeline, and a water inlet valve is provided on the interlayer water circulation inlet pipeline. When the interlayer is heated, the drain valve of the interlayer steam outlet pipeline is opened, and when the interlayer water is circulated, the drain valve of the interlayer steam outlet pipeline is closed.
[0020] Furthermore, the feeding device specifically includes an inoculation device, a cleaning device and a material feeding device, all of which are arranged on the top of the cultivation tank body, and the inoculation device includes an inoculation port and an inoculation cover arranged on the inoculation port; the cleaning device is a multi-axis manipulator and a spray device arranged at the end of the multi-axis manipulator, and the multi-axis manipulator drives the spray device to move in the cultivation tank body.
[0021] Furthermore, the culture tank is also provided with a stirring device, the stirring device comprising a stirring shaft, a stirring blade and a motor, one end of the stirring shaft is connected to the motor, and the other end is connected to the stirring blade.
[0022] Furthermore, a sampling port is also provided on the cultivation tank.
[0023] The above technical solution includes at least the following technical effects:
[0024] It can be seen from the above technical solutions that the present invention discloses a multi-tank breeding device for liquid edible fungi. Compared with the prior art, the present invention has the following advantages:
[0025] Large-scale strain cultivation capability: The present invention provides a multi-tank breeding device for edible fungi liquid strains, which is capable of large-scale strain cultivation, solving the technical problem that traditional strain cultivation devices cannot carry out large-scale cultivation.
[0026] Precise environment and temperature control: Through the cultivation environment control device and the cultivation temperature control device, the cultivation environment and temperature in the cultivation tank can be accurately regulated to provide suitable growth conditions for the strains.
[0027] Efficient strain transportation: A strain transfer device is provided at the bottom of the strain cultivation device, which can effectively transport strains to the connected strain cultivation device, thereby improving the efficiency of strain cultivation.
[0028] Sterilization and clean air supply: Through the high-pressure clean gas input pipeline and sterilization device, the high-pressure air can be sterilized to ensure the cleanliness of the culture environment and prevent contamination.
[0029] Automated and intelligent operation: The feeding device includes an inoculation device, a cleaning device and a material feeding device, as well as a stirring device and a sampling port, making the strain cultivation process more automated and intelligent.
[0030] Multi-stage cultivation capability: The strain cultivation device includes fermentation cultivation devices of different capacities, which can realize multi-stage strain cultivation from small to large.
[0031] Flexibility of temperature regulation: Through the setting of the interlayer steam inlet pipeline and the interlayer water circulation pipeline, the temperature in the fermentation tank can be flexibly adjusted, including raising the temperature, lowering the temperature and maintaining the temperature.
[0032] Diversity of environmental adjustment: Through the setting of exhaust pipes inside the tank, steam pipes inside the tank, high-pressure air pipes inside the tank and steam sterilization pipes for the tank filter element, a variety of environmental adjustment operations can be performed to meet different cultivation needs.
[0033] Prevent secondary contamination: By timely transferring the sterilized culture medium to the strain culture device, secondary contamination of the culture medium is avoided, thereby improving the success rate of strain cultivation. BRIEF DESCRIPTION OF THE DRAWINGS
[0034] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying creative work.
[0035] Figure 1 The present invention is a schematic diagram of the overall structure of a strain cultivation device of a multi-tank breeding system for edible fungi liquid strains.
[0036] Figure 2 It is a rear structural schematic diagram of a strain cultivation device of a multi-tank breeding system for liquid edible fungi of the present invention.
[0037] Figure 3 It is a schematic diagram of the structure of another strain cultivation device of the edible fungus liquid strain multi-tank breeding system of the present invention.
[0038] Among them, 1 is a cultivation tank, 2 is a cultivation environment control device, 3 is a cultivation temperature control device, 4 is a feeding device, 5 is a seed transplanting device, 6 is a stirring device, 7 is a sampling port, 21 is a first high-temperature steam input pipeline, 22 is a high-pressure clean gas input pipeline, 23 is a pressure detection device, 24 is an outlet valve, 25 is an air flow meter, 26 is a sewage valve, 27 is a drainage valve, 28 is a flow meter valve, 31 is an interlayer steam inlet pipeline, 32 is an interlayer water circulation outlet pipeline, 3 3 is the interlayer steam outlet pipeline, 34 is the interlayer water circulation inlet pipeline, 41 is the inoculation device, 211 is the steam main valve, 212 is the steam valve, 213 is the steam exhaust valve, 221 is the high-pressure air input pipeline, 222 is the second high-temperature steam input pipeline, 223 is the sterilization device, 224 is the air main valve, 225 is the clean air valve, 226 is the air filter exhaust valve, 227 is the air filter steam valve, 228 is the clean air steam valve, 411 is the inoculation port, and 412 is the inoculation cover. DETAILED DESCRIPTION
[0039] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.
[0040] Referring to the accompanying drawings, an embodiment of the present invention discloses a multi-tank breeding system for liquid edible fungi, comprising: a plurality of edible fungi cultivation devices connected in sequence;
[0041] The strain cultivation device comprises a cultivation tank 1, a cultivation environment control device 2, a cultivation temperature control device 3 and a feeding device 4. The feeding device 4 is fixedly installed on the top of the cultivation tank 1 and is used to feed raw materials. The cultivation environment control device 2 is connected to the inside of the cultivation tank 1 and is used to adjust the cultivation environment inside the cultivation tank 1. The cultivation temperature control device 3 is connected to the hollow cavity wall of the cultivation tank 1 and is used to control the cultivation temperature inside the cultivation tank 1 through high-temperature gas or low-temperature liquid.
[0042] A seed transfer device 5 is provided at the bottom of the strain cultivation device, and the strains are transported to the connected strain cultivation device through the seed transfer device 5 .
[0043] To further optimize the above technical solution, the cultivation environment control device 2 includes an input device and an exhaust device. The input device includes a first high-temperature steam input pipeline 21 and a high-pressure clean gas input pipeline 22. The first high-pressure steam input pipeline 21 and the high-pressure clean gas input pipeline 22 are connected to the cultivation tank body 1 through valves.
[0044] In order to further optimize the above technical solution, a steam main valve 211 and a steam valve 212 are provided on the first high-temperature steam input pipeline 21 , and a steam exhaust valve 213 is also provided between the steam main valve 211 and the steam valve 212 .
[0045] To further optimize the above technical solution, the high-pressure clean gas input pipeline 22 includes a high-pressure air input pipeline 221 and a second high-temperature steam input pipeline 222. A sterilization device 223 is provided on the high-pressure air input pipeline 221. The second high-temperature steam input pipeline 22 is connected to the sterilization device 223 for sterilizing the sterilization device 223 with high-temperature steam. The sterilization device 223 is used to sterilize the high-pressure air in the high-pressure air input pipeline 221. An air main valve 224, a clean air valve 225 and an air filter exhaust valve 226 are provided on the high-pressure air input pipeline 221. A plurality of air filter steam valves 227 are provided on the second high-pressure steam input pipeline 222.
[0046] In order to further optimize the above technical solution, the sterilizing device 223 is also provided with a pressure detection device 23 and a drain valve 27;
[0047] To further optimize the above technical solution, the discharge device includes a discharge pipeline and a pressure detection device 23, an air outlet main valve 24, an air flow meter 25 and a drain valve 26 which are sequentially arranged on the discharge pipeline. The air flow meter 25 is also provided with a drain valve 27.
[0048] To further optimize the above technical solution, the cultivation temperature control device 3 specifically includes an interlayer steam inlet pipeline 31, an interlayer water circulation outlet pipeline 32, an interlayer steam outlet pipeline 33 and an interlayer water circulation inlet pipeline 34;
[0049] A steam valve 311 is provided on the interlayer steam inlet pipeline 31, a drain valve 27 is provided on the interlayer water circulation outlet pipeline 32, a drain valve 27 is provided on the interlayer steam outlet pipeline 33, and an inlet valve 341 is provided on the interlayer water circulation inlet pipeline 34. When the interlayer is heated, the drain valve 27 of the interlayer steam outlet pipeline 33 is opened, and when the interlayer water is circulated, the drain valve 27 of the interlayer steam outlet pipeline 33 is closed.
[0050] To further optimize the above technical solution, the feeding device 4 specifically includes an inoculation device 41, a cleaning device and a material feeding device 42, all of which are arranged on the top of the cultivation tank body 1, and the inoculation device 41 includes an inoculation port 411 and an inoculation cover 412 arranged on the inoculation port 411; the cleaning device is a multi-axis manipulator and a spray device arranged at the end of the multi-axis manipulator, and the multi-axis manipulator drives the spray device to move in the cultivation tank body.
[0051] In order to further optimize the above technical solution, a stirring device 6 is also provided on the cultivation tank 1, and the stirring device 6 includes a stirring shaft, a stirring blade and a motor, one end of the stirring shaft is connected to the motor, and the other end is connected to the stirring blade.
[0052] In order to further optimize the above technical solution, a sampling port 7 is also provided on the cultivation tank body 1 .
[0053] like Figure 1 As shown, the bacterial culture device includes a 100L fermentation culture device, a 500L fermentation culture device and a Figure 3 In the 5000L fermentation and cultivation device shown, the 100L fermentation device is connected to the feed pipeline of the 500L fermentation device through the discharge pipeline, and the 500L fermentation device is connected to the feed pipeline of the 5000L fermentation device through the discharge pipeline. The fermented bacteria flow to the next fermentation device through the discharge pipeline and the feed pipeline. After the fermentation is completed, the 5000L fermentation device outputs the final fermentation product;
[0054] The 100L fermentation device includes a culture tank body 1 with a sandwich, and a feeding device 4, an inoculation port 411 and an inoculation cleaning port are arranged on the top of the culture tank body 1. An inoculation cover 412 is arranged on the inoculation port 411. The inoculation port 411 is used to inoculate the inside of the culture tank body 1, and the inoculation cleaning port is used to clean the inside of the culture tank body 1 after the strain cultivation is completed;
[0055] When bacterial strain cultivation is required, a certain amount of culture medium is put in through the feeding device 4, bacterial strains are put in through the inoculation port 411, the feeding device 4 and the inoculation cover 412 are closed, and then the temperature of the fermentation tank is adjusted through the cultivation temperature control device 3 and the cultivation environment is adjusted through the cultivation environment control device 2;
[0056] Specifically, the cultivation temperature control device 3 includes an interlayer steam inlet pipeline 31, an interlayer water circulation outlet pipeline 32, an interlayer steam outlet pipeline 33, and an interlayer water circulation inlet pipeline 34;
[0057] Among them, the interlayer water circulation outlet pipeline 32 is a plurality of pipes, the ends of which are connected to the upper half of the interlayer of the cultivation tank body 1, one pipe is provided with a manual cooling outlet valve, one pipe is provided with an automatic cooling outlet valve, and one pipe is provided with an automatic heating outlet valve;
[0058] The interlayer water circulation inlet pipeline 34 is a plurality of pipelines, the ends of which are connected to the lower half of the interlayer of the fermentation tank, one pipeline is provided with an automatic heating inlet valve, one pipeline is provided with an automatic cooling inlet valve, and one pipeline is provided with a manual cooling inlet valve;
[0059] Temperature regulation of the cultivation tank 1, including raising the temperature, lowering the temperature and maintaining the temperature;
[0060] When the temperature needs to be raised, close the manual cold zone outlet valve, automatic cooling outlet valve and automatic heating outlet valve on the interlayer water circulation outlet pipe 32, close the automatic heating inlet valve, automatic cooling inlet valve and manual cooling inlet valve on the interlayer water circulation inlet pipe 34, close the jacket steam valve on the interlayer steam inlet pipe 31, and open the jacket drain valve on the interlayer steam outlet pipe 33; high-temperature steam enters the interlayer of the cultivation tank body 1 from the interlayer steam inlet pipe 31 and is discharged from the interlayer steam outlet pipe 33 to achieve heating of the fermentation tank;
[0061] When the temperature needs to be lowered or maintained, the manual cooling inlet valve on the interlayer water circulation inlet pipeline 34 is manually opened or the automatic cooling inlet valve and the automatic heating inlet valve are automatically opened, the spacer drain valve on the interlayer steam outlet pipeline 33 is closed, the insulated steam valve on the interlayer steam inlet pipeline 31 is closed, and the manual cooling outlet valve, the automatic cold zone outlet valve or the automatic heating outlet valve on the interlayer water circulation outlet pipeline 32 is opened;
[0062] The temperature inside the cultivation tank 1 is regulated by manually or automatically opening and closing the multiple valves on the cultivation temperature control device 3 .
[0063] The cultivation environment control device 2 includes a first high-temperature steam input pipeline 21, a high-pressure clean gas input pipeline 22, a second high-temperature steam input pipeline 222 and an exhaust pipeline in the tank;
[0064] The first high-temperature steam input pipeline 21 is provided with a steam main valve 211, a steam valve 212 and a steam exhaust valve 213, the high-pressure air input pipeline 221 is provided with an air main valve 224, a sterilizing device 223, a clean air valve 225 and a clean air steam valve 228, the second high-temperature steam input pipeline 222 is provided with a plurality of air filter steam valves 227 and a drain valve 27, and the exhaust pipeline in the tank is provided with a pressure detection device 23, an air outlet main valve 24, an air flow meter 25, a drain valve 26 and a drain valve 27;
[0065] When high-pressure steam needs to be introduced, the steam exhaust valve 213 on the first high-temperature steam input pipeline 21 is closed, the steam main valve 211, the steam valve 212 and the clean air steam valve 228 are opened, and the air filter exhaust valve 226 is closed;
[0066] When high-pressure air needs to be introduced, the main air valve 224, the clean air valve 225 and the clean air steam valve 228 on the high-pressure air input pipeline 221 are opened;
[0067] The second high-temperature steam input pipeline 222 is used to sterilize the sterilization device 223. Specifically, the plurality of air filter steam valves 227 and the drain valve 27 on the second high-temperature steam input pipeline 222 are opened to sterilize the sterilization device 223 by high-temperature steam.
[0068] When it is necessary to obtain the steam or high-pressure air flow in the cultivation tank body 1, open the main air outlet valve 24 and the flow meter valve 28; when it is necessary to discharge the steam or air in the cultivation tank body 1, open the main air outlet valve 24 and the drain valve 26, close the flow meter valve 28 and the drain valve 27; when it is necessary to drain the flow meter, open the flow meter valve 28 and the drain valve 27;
[0069] Through the above process, the strain cultivation in the 100L fermenter is achieved. After the cultivation is completed, the strain is transferred to the 500L fermenter through the strain transfer pipeline at the bottom of the fermenter. The strain transfer pipeline is provided with a main discharge valve and a strain transfer valve for further expansion and cultivation.
[0070] The adjustment process and structure of the 500L fermentation and cultivation device are the same as those of the 100L fermentation and cultivation device, and the same control pipeline is used for further cultivation of the strains. The same structure will not be further described. After the cultivation is completed, the cultivated strains are transferred to the 5000L fermentation and cultivation device through the transfer pipeline;
[0071] like Figure 3 As shown, it is a 5000L fermentation and cultivation device, which is further provided with the following devices compared with the 100L fermentation and cultivation device and the 500L fermentation and cultivation device:
[0072] A stirring device 6 is arranged on the top of the cultivation tank 1, and the stirring device 6 includes a motor, a stirring shaft and a stirring blade. The motor drives the stirring shaft to rotate, and the stirring shaft drives the stirring blade to stir the bacterial strain in the cultivation tank 1. The seed transfer device 5 includes a seed transfer pipeline and a plurality of steam sterilization drain valves and an inlet valve arranged on the pipeline. When the seed transfer is performed, the steam sterilization drain valve is closed. When the seed transfer pipeline is sterilized, the steam sterilization drain valve is opened to discharge the sterilized waste water from the steam sterilization drain valve.
[0073] A sampling port 7 is installed on the side wall of the cultivation tank body 1. The sampling port 7 includes a fermentation tank sampling pipeline and a sampling port steam main valve, a sampling port steam condensate water drain valve and a sampling port tank internal discharge main valve arranged on the pipeline. When it is necessary to take materials, the sampling port steam main valve is closed, and the sampling port steam condensate water drain valve and the sampling port tank internal discharge main valve are opened. After sampling, the sampling port 7 is steam-sterilized. At this time, the sampling port tank internal discharge main valve is closed, and the sampling port steam main valve and the sampling port steam condensate water drain valve are opened.
[0074] A plurality of sensors are also provided in the cultivation tank 1, including temperature sensors and humidity sensors, etc., which are used to monitor the temperature and humidity inside the cultivation tank 1, and control the opening or closing of the automatic valve based on preset data, thereby automatically adjusting the cultivation environment of the strain, realizing automatic cultivation of the strain, and improving the cultivation efficiency.
[0075] The present invention provides a multi-tank breeding system for liquid edible fungi, which can carry out large-scale cultivation of fungi and solve the technical problem that traditional fungi cultivation devices cannot carry out large-scale cultivation; through a cultivation environment control device and a cultivation temperature control device, the cultivation environment and temperature in the cultivation tank can be accurately regulated to provide suitable growth conditions for the fungi; a seed transfer device is arranged at the bottom of the fungi cultivation device, which can effectively transport fungi to the connected fungi cultivation device, thereby improving the efficiency of fungi cultivation; through a high-pressure clean gas input pipeline and a sterilizing device, high-pressure air can be sterilized to ensure the cleanliness of the fungi cultivation environment and prevent pollution; the feeding device includes an inoculation device, a cleaning device and a material feeding device, and The setting of the stirring device and the sampling port makes the strain cultivation process more automated and intelligent; the strain cultivation device includes fermentation cultivation devices of different capacities, which can realize multi-stage strain cultivation from small to large; through the setting of the interlayer steam air inlet pipeline and the interlayer water circulation pipeline, the temperature in the fermentation tank can be flexibly adjusted, including raising the temperature, lowering the temperature and maintaining the temperature; through the setting of the exhaust pipe in the tank, the steam inlet pipe in the tank, the high-pressure air inlet pipe in the tank and the tank filter steam sterilization pipe, a variety of environmental adjustment operations can be performed to meet different cultivation needs; by timely transferring the sterilized culture medium to the strain cultivation device, secondary contamination of the culture medium is avoided, thereby improving the success rate of strain cultivation.
[0076] The various embodiments in this specification are described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the various embodiments can be referenced to each other.
[0077] The above description of the disclosed embodiments enables one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.
Claims
1. A multi-tank breeding system for liquid edible fungi, characterized in that: include: A plurality of bacterial strain cultivation devices connected in sequence; The bacterial strain cultivation device comprises a cultivation tank body (1), a cultivation environment control device (2), a cultivation temperature control device (3) and a feeding device (4); the feeding device (4) is fixedly mounted on the top of the cultivation tank body (1) and is used to feed raw materials; the cultivation environment control device (2) is connected to the inside of the cultivation tank body (1) and is used to regulate the cultivation environment inside the cultivation tank body (1); the cultivation temperature control device (3) is connected to the hollow cavity wall of the cultivation tank body (1) and is used to control the cultivation temperature inside the cultivation tank body (1) through high-temperature gas or low-temperature liquid; A strain transfer device (5) is provided at the bottom of the strain cultivation device, and strains are transported to the connected strain cultivation device through the strain transfer device (5).
2. The edible fungus liquid spawn multi-tank breeding system according to claim 1, characterized in that: The cultivation environment control device (2) comprises an input device and an exhaust device, wherein the input device comprises a first high-temperature steam input pipeline (21) and a high-pressure clean gas input pipeline (22), and the first high-pressure steam input pipeline (21) and the high-pressure clean gas input pipeline (22) are connected to the cultivation tank body (1) via valves.
3. The edible fungus liquid spawn multi-tank breeding system according to claim 2, characterized in that: A steam main valve (211) and a steam valve (212) are provided on the first high-temperature steam input pipeline (21), and a steam exhaust valve (213) is also provided between the steam main valve (211) and the steam valve (212).
4. The edible fungus liquid spawn multi-tank breeding system according to claim 2, characterized in that: The high-pressure clean gas input pipeline (22) comprises a high-pressure air input pipeline (221) and a second high-temperature steam input pipeline (222); a sterilizing device (223) is provided on the high-pressure air input pipeline (221); the second high-temperature steam input pipeline (222) is connected to the sterilizing device (223) and is used to sterilize the sterilizing device (223) by means of high-temperature steam; the sterilizing device (223) is used to sterilize the high-pressure air in the high-pressure air input pipeline (221); an air main valve (224), a clean air valve (225), an air filter exhaust valve (226) and a clean air steam valve (228) are provided on the high-pressure air input pipeline (221); and a plurality of air filter steam valves (227) are provided on the second high-pressure steam input pipeline (222).
5. The edible fungus liquid spawn multi-tank breeding system according to claim 4, characterized in that: The sterilizing device (223) is also provided with a pressure detection device (23) and a drainage valve (27).
6. The edible fungus liquid spawn multi-tank breeding system according to claim 2, characterized in that: The discharge device comprises a discharge pipeline and a pressure detection device (23), an air outlet main valve (24), an air flow meter (25) and a drain valve (26) which are sequentially arranged on the discharge pipeline. The air flow meter (25) is also provided with a drain valve (27).
7. The edible fungus liquid spawn multi-tank breeding system according to claim 1, characterized in that: The cultivation temperature control device (3) specifically comprises an interlayer steam inlet pipeline (31), an interlayer water circulation outlet pipeline (32), an interlayer steam outlet pipeline (33) and an interlayer water circulation inlet pipeline (34); The interlayer steam inlet pipeline (31) is provided with a steam valve (311), the interlayer water circulation outlet pipeline (32) is provided with a drain valve (27), the interlayer steam outlet pipeline (33) is provided with the drain valve (27), and the interlayer water circulation inlet pipeline (34) is provided with a water inlet valve (341). When the interlayer is heated, the drain valve (27) of the interlayer steam outlet pipeline (33) is opened, and when the interlayer water is circulated, the drain valve (27) of the interlayer steam outlet pipeline (33) is closed.
8. The edible fungus liquid spawn multi-tank breeding system according to claim 1, characterized in that: The feeding device (4) specifically includes an inoculation device (41), a cleaning device and a material feeding device (42), all of which are arranged on the top of the cultivation tank body (1), and the inoculation device (41) includes an inoculation port (411) and an inoculation cover (412) arranged on the inoculation port (411); the cleaning device is a multi-axis manipulator and a spray device arranged at the end of the multi-axis manipulator, and the multi-axis manipulator is used to drive the spray device to move in the cultivation tank body.
9. The edible fungus liquid spawn multi-tank breeding system according to claim 1, characterized in that: The culture tank body (1) is also provided with a stirring device (6), which comprises a stirring shaft, a stirring blade and a motor. One end of the stirring shaft is connected to the motor, and the other end is connected to the stirring blade.
10. The edible fungus liquid spawn multi-tank breeding system according to claim 1, characterized in that: The cultivation tank body (1) is also provided with a sampling port (7).
Citation Information
Patent Citations
A cultivation device for edible fungi strains and a cultivation method for edible fungi strains.
CN109328883B
Pleurotus eryngii liquid strain culture device
CN109429912A
