Preparation method of medicine for treating arthralgia and myalgia
The supercritical fluid extraction technology extracts Chinese herbal ingredients and prepares efficient medicines, which solves the problem of poor efficacy in treating muscle and bone pain, and achieves rapid and significant pain relief and quality of life improvement.
Patent Information
- Application Number
- CN202411646289.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-18
- Publication Date
- 2025-03-25
AI Technical Summary
The existing drugs have poor efficacy, side effects, inconvenient use, and long treatment cycles for muscle and bone pain.
Using supercritical fluid extraction technology, Chinese herbal medicines such as Deep Mountains, Weilingxian, Chicken Blood Vine are mixed with carbon dioxide for extraction to remove impurities and prepare efficient medicinal liquid.
Significantly relieves bone and muscle pain, quickly improves patient symptoms, promotes repair of damaged tissues, and has quick results and significant results, alleviates patients' pain and improves quality of life.
Smart Images
Figure CN119679874A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the technical field of prescription preparation, and in particular to a method for preparing a medicine for treating muscle and bone pain. Background Art
[0002] With the changes in modern lifestyles, people sit for long periods of time, lack of exercise, and aging, the incidence of muscle and bone pain-related diseases has increased year by year. Problems with waist and knee pain, lumbar disc herniation, poor muscle relaxation, bone hyperplasia, bruises, muscle strain, flexion and extension problems, rheumatism, etc. are common clinical diseases and common health and disease problems that bring great pain to patients and seriously affect their quality of life.
[0003] Currently, common treatment methods include physical therapy, drug therapy, and surgical treatment. However, physical therapy has limited effects, and surgical treatment has high risks, high costs, and is prone to recurrence.
[0004] However, existing drug treatments often have poor efficacy, side effects, inconvenience in use, and long treatment cycles, and there are still certain deficiencies in the treatment effects and usability of some more serious diseases. Summary of the invention
[0005] The main purpose of the present invention is to provide a method for preparing a medicine for treating muscle and bone pain, which can effectively solve the problems that existing treatments often have poor efficacy, side effects, inconvenience in use, and long treatment cycles.
[0006] To achieve the above object, the technical solution adopted by the present invention is:
[0007] A method for preparing a medicine for treating muscle and bone pain, the method comprising:
[0008] S1. Select the above-mentioned medicinal ingredients that meet the quality standards, fresh and high-quality Chinese herbal medicines, wash, dry, inspect and crush them to make them suitable for extraction;
[0009] S2, mixing the crushed Chinese medicinal materials with an extractant and performing supercritical fluid extraction;
[0010] S3, separating and purifying the extract to remove impurities.
[0011] Preferably, the Chinese herbal medicine comprises a composition A and a composition B:
[0012] The formula medicine A comprises: Shen Shan Bu Tu Tou, Clematis chinensis, Millettia spatholobi, Morus alba, Caulis Piperis ternata, Millennium Jian, and Stretching Tendon Grass;
[0013] The prescription medicine B includes: Chuanxiong, safflower, Notopterygium wilfordii, frankincense, agarwood, myrrh, natural copper, Achyranthes bidentata, mistletoe, raw Chuanwu, Dipsacus asper, Drynaria fortunei, Angelica dahurica, Arisaema consanguineum, Scorpio, Panax notoginseng, and clove basil oil.
[0014] Preferably, in the pulverizing process described in S2, the medicinal material is pulverized into 50-80 meshes, and the impurities therein are screened out through a sieve.
[0015] Preferably, the extraction agent used in the extraction process in S2 is carbon dioxide, and its purity is not less than 99.9%.
[0016] Preferably, the working pressure of the carbon dioxide during the extraction process is 10-30 MPa, and the temperature of the carbon dioxide during the extraction process is 35-45°C.
[0017] Preferably, 5-15 qian of Shenshan Butoutou, 10-20 qian of Clematis, 8-18 qian of Millettia reticulata, 12-22 qian of Morus alba, 8-18 qian of Piper melongena, 5-15 qian of Qiannianjian, and 10-20 qian of Sesamum indica.
[0018] Preferably, 10-20 qian of Chuanxiong, 8-18 qian of safflower, 5-15 qian of Notopterygium, 8-18 qian of frankincense, 5-15 qian of agarwood, 8-18 qian of myrrh, 5-15 qian of natural copper, 10-20 qian of Achyranthes bidentata, 8-18 qian of mistletoe, 5-15 qian of raw Chuanwu, 10-20 qian of Dipsacus asper, 8-18 qian of Drynariae Rhizoma, 5-15 qian of Angelicae Pubescentis, 10-20 qian of Arisaema, 3-12 qian of Scorpio, 8-18 qian of Panax notoginseng, and 2-10 qian of clove basil oil.
[0019] Preferably, in the supercritical fluid extraction process described in S2, the ratio of Chinese medicinal materials to the extractant is 1:5 to 1:10.
[0020] Preferably, the separation and purification step in S3 is achieved by centrifugation, and the centrifuge speed is 3000-5000 rpm.
[0021] Preferably, the impurity removal process in S3 is filtered using a 0.2 micron filter membrane.
[0022] Compared with the prior art, the present invention has the following beneficial effects:
[0023] 1. The present invention has significant therapeutic effects on muscle and bone pain, waist and knee joint diseases, lumbar disc herniation, rheumatic pain, poor muscle relaxation, bone hyperplasia, traumatic injuries and muscle strain-related diseases; it can relieve pain safely, effectively and quickly, can penetrate into the lesions to improve the patient's symptoms, promote the repair of damaged tissues, and has a fast effect, high performance, and obvious and outstanding effects to reduce the patient's pain, and can effectively improve the patient's health and quality of life.
[0024] 2. The present invention can achieve significant therapeutic effects in a relatively short period of time, reduce the patient's treatment costs and time costs, and provide patients with a safe and reliable treatment option.
[0025] 3. The present invention uses a variety of natural herbs as raw materials, does not contain harmful chemicals, has no pollution to the environment, meets the needs of modern people for green and healthy products, and has broad market prospects.
[0026] 4. The medicinal liquid prepared by the present invention can be used in the form of spray, ointment, patch or oral administration. Through the characteristics, effects and applicable scenarios of different usage methods, symptomatic medication can be adaptively adjusted according to the patient's medication habits and the severity of the disease, thereby meeting the needs of different patients. BRIEF DESCRIPTION OF THE DRAWINGS
[0027] Figure 1 It is a flow chart of the preparation process of the present invention. DETAILED DESCRIPTION
[0028] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention is further explained below in conjunction with specific implementation methods.
[0029] The present invention mainly relates to a method for preparing a medicine for treating muscle and bone pain, the method comprising:
[0030] S1. Select the above-mentioned medicinal ingredients that meet the quality standards, fresh and high-quality Chinese herbal medicines, wash, dry, inspect and crush them to make them suitable for extraction;
[0031] S2, mixing the crushed Chinese medicinal materials with an extractant and performing supercritical fluid extraction;
[0032] S3, separating and purifying the extract to remove impurities.
[0033] Chinese herbal medicines include formula A and formula B:
[0034] The formula A includes: Shen Shan Bu Tu Tou, Clematis chinensis, Millettia reticulata, Morus alba, Caulis Piperis ternata, Millennium Jian, and Stretching Tendon Grass;
[0035] Composition medicine B includes: Chuanxiong, safflower, Qianghuo, frankincense, agarwood, myrrh, natural copper, Achyranthes bidentata, mistletoe, raw Chuanwu, Dipsacus asper, Drynaria fortunei, Duhuo, Arisaema, scorpion, Panax notoginseng, and clove basil oil.
[0036] During the pulverization process in S2, the medicinal material is pulverized into 50-80 meshes, and the impurities therein are screened out through a sieve.
[0037] The extraction solvent used in the extraction process in S2 is carbon dioxide, and its purity is not less than 99.9%.
[0038] The working pressure of carbon dioxide during the extraction process is 10-30MPa, and the temperature of carbon dioxide during the extraction process is 35-45°C.
[0039] 5-15 coins for Deep Mountain, 10-20 coins for Clematis, 8-18 coins for Millettia reticulata, 12-22 coins for Morus alba, 8-18 coins for Caulis Piperis, 5-15 coins for Millennium Health, and 10-20 coins for Smilax glabra.
[0040] Ligusticum chuanxiong 10-20 qian, safflower 8-18 qian, angelica dahurica 5-15 qian, frankincense 8-18 qian, agarwood 5-15 qian, myrrh 8-18 qian, natural copper 5-15 qian, Achyranthes bidentata 10-20 qian, mistletoe 8-18 qian, raw Chuanwu 5-15 qian, Dipsacus asper 10-20 qian, Drynaria fortunei 8-18 qian, Duhuo 5-15 qian, Arisaema 10-20 qian, Scorpio 3-12 qian, Panax notoginseng 8-18 qian, clove basil oil 2-10 qian.
[0041] During the supercritical fluid extraction process in S2, the ratio of Chinese medicinal materials to the extractant was 1:5 to 1:10.
[0042] The separation and purification step in S3 is achieved by centrifugation, and the centrifuge speed is 3000-5000 rpm.
[0043] The S3 impurity removal process uses a 0.2 micron filter membrane for filtration.
[0044] Among them, supercritical fluid extraction (SFE) is a technology that uses supercritical fluid as an extractant to selectively extract specific components from solids or liquids. In the supercritical state, the density and solubility of the fluid are extremely sensitive to changes in temperature and pressure, so the extraction process can be controlled by adjusting these parameters. Specifically, when the temperature and pressure of the fluid exceed its critical point, the fluid exhibits both the fluidity of a gas and the high solubility of a liquid, thereby being able to effectively extract the target substance. Supercritical fluid extraction has the advantages of simple operation, high extraction efficiency, and no solvent residue, and is therefore widely used in the fields of medicine, food, and cosmetics, becoming an important part of the prior art, and has been widely used in the prior art. Its specific structure and operating principle will no longer be displayed and explained in the present invention.
[0045] According to the method for preparing a drug for treating muscle and bone pain proposed in the present invention, there are many optional factors, and a variety of embodiments can be designed. Therefore, the specific embodiments are only used as illustrative descriptions of the specific implementation methods of the present invention, and do not constitute a limitation on the scope of the present invention.
[0046] Embodiment 1, select Shenshan Butoutou 5 money, Clematis 10 money, Millettia 8 money, Morus 12 money, Caulis Piperis 8 money, Qiannianjian 5 money, Lycopodiella ciliata 10 money;
[0047] As well as 10 qian of Chuanxiong, 8 qian of Safflower, 5 qian of Qianghuo, 8 qian of Frankincense, 5 qian of Agarwood, 8 qian of Myrrh, 5 qian of Natural Copper, 10 qian of Achyranthes bidentata, 8 qian of Viscum album, 5 qian of Raw Chuanwu, 10 qian of Dipsacus asper, 8 qian of Drynaria fortunei, 5 qian of Duhuo, 10 qian of Arisaema, 3 qian of Scorpion, 8 qian of Panax notoginseng, and 2 qian of clove and basil oil.
[0048] The above medicinal materials were cleaned, dried, inspected and crushed to 80 mesh, and impurities were sieved through a sieve.
[0049] The crushed Chinese medicinal materials are mixed with an extractant (carbon dioxide with a purity of not less than 99.9%) and subjected to supercritical fluid extraction. The working pressure during the extraction process is 10 MPa and the temperature is 35°C.
[0050] The extract was separated and purified by a centrifuge (rotating speed of 3000 rpm) to remove impurities.
[0051] The separated and purified extract was filtered using a 0.2 μm filter membrane to remove remaining impurities.
[0052] Embodiment 2, select Shenshan Butoutou 15 money, clematis 20 money, Millettia spatholobi 18 money, mulberry twig 22 money, sea wind vine 18 money, Qiannianjian 15 money, lycopodiella ciliata 20 money;
[0053] As well as 20 qian of Chuanxiong, 18 qian of safflower, 15 qian of Qianghuo, 18 qian of frankincense, 15 qian of agarwood, 18 qian of myrrh, 15 qian of natural copper, 20 qian of Achyranthes bidentata, 18 qian of mistletoe, 15 qian of raw Chuanwu, 20 qian of Dipsacus asper, 18 qian of Drynaria fortunei, 15 qian of Duhuo, 20 qian of Arisaema, 12 qian of whole scorpion, 18 qian of Panax notoginseng, and 10 qian of clove and basil oil.
[0054] The above medicinal materials were cleaned, dried, inspected and crushed to 80 mesh, and impurities were sieved through a sieve.
[0055] The crushed Chinese medicinal materials are mixed with an extractant (carbon dioxide with a purity of not less than 99.9%) and subjected to supercritical fluid extraction. The working pressure during the extraction process is 10 MPa and the temperature is 35°C.
[0056] The extract was separated and purified by a centrifuge (rotating speed of 3000 rpm) to remove impurities.
[0057] The separated and purified extract was filtered using a 0.2 μm filter membrane to remove remaining impurities.
[0058] Embodiment three, select Shenshan Butoutou 10 money, Clematis 15 money, Millettia spatholobi 13 money, Morus 17 money, Caulis Piperis 13 money, Qiannianjian 10 money, Lycopodiella ciliata 15 money;
[0059] As well as 15 qian of Chuanxiong, 13 qian of Safflower, 10 qian of Qianghuo, 13 qian of Frankincense, 10 qian of Agarwood, 13 qian of Myrrh, 10 qian of Natural Copper, 15 qian of Achyranthes bidentata, 13 qian of Viscum album, 10 qian of Raw Chuanwu, 15 qian of Dipsacus asper, 13 qian of Drynaria fortunei, 10 qian of Duhuo, 15 qian of Arisaema, 7 qian of Scorpion, 13 qian of Panax notoginseng, and 6 qian of clove and basil oil.
[0060] The above medicinal materials were cleaned, dried, inspected and crushed to 80 mesh, and impurities were sieved through a sieve.
[0061] The crushed Chinese medicinal materials are mixed with an extractant (carbon dioxide with a purity of not less than 99.9%) and subjected to supercritical fluid extraction. The working pressure during the extraction process is 10 MPa and the temperature is 35°C.
[0062] The extract was separated and purified by a centrifuge (rotating speed of 3000 rpm) to remove impurities.
[0063] The separated and purified extract was filtered using a 0.2 μm filter membrane to remove remaining impurities.
[0064] Embodiment 4, select Shenshan Butoutou 12 money, Clematis 18 money, Millettia 18 money, Morus 15 money, Caulis Piperis 12 money, Qiannianjian 8 money, and Lycopodiella ciliata 12 money;
[0065] As well as 20 qian of Chuanxiong, 18 qian of safflower, 8 qian of Qianghuo, 12 qian of frankincense, 15 qian of agarwood, 18 qian of myrrh, 8 qian of natural copper, 12 qian of Achyranthes bidentata, 10 qian of mistletoe, 8 qian of raw Chuanwu, 12 qian of Dipsacus asper, 10 qian of Drynaria, 8 qian of Duhuo, 12 qian of Arisaema, 5 qian of whole scorpion, 18 qian of Panax notoginseng, and 4 qian of clove basil oil.
[0066] The above medicinal materials were cleaned, dried, inspected and crushed to 80 mesh, and impurities were sieved through a sieve.
[0067] The crushed Chinese medicinal materials are mixed with an extractant (carbon dioxide with a purity of not less than 99.9%) and subjected to supercritical fluid extraction. The working pressure during the extraction process is 10 MPa and the temperature is 35°C.
[0068] The extract was separated and purified by a centrifuge (rotating speed of 3000 rpm) to remove impurities.
[0069] The separated and purified extract was filtered using a 0.2 μm filter membrane to remove remaining impurities.
[0070] Embodiment 5, select Shenshan Butoutou 8 money, Clematis 12 money, Millettia 14 money, Morus 20 money, Caulis Piperis 10 money, Millennium Jian 10 money, and Lycopodiella 18 money;
[0071] As well as 18 qian of Chuanxiong, 12 qian of Safflower, 10 qian of Qianghuo, 15 qian of Frankincense, 12 qian of Agarwood, 10 qian of Myrrh, 12 qian of Natural Copper, 18 qian of Achyranthes bidentata, 14 qian of Viscum album, 10 qian of Raw Chuanwu, 15 qian of Dipsacus asper, 12 qian of Drynaria fortunei, 12 qian of Duhuo, 18 qian of Arisaema, 9 qian of Scorpion, 12 qian of Panax notoginseng, and 8 qian of clove and basil oil.
[0072] The above medicinal materials were cleaned, dried, inspected and crushed to 80 mesh, and impurities were sieved through a sieve.
[0073] The crushed Chinese medicinal materials are mixed with an extractant (carbon dioxide with a purity of not less than 99.9%) and subjected to supercritical fluid extraction. The working pressure during the extraction process is 10 MPa and the temperature is 35°C.
[0074] The extract was separated and purified by a centrifuge (rotating speed of 3000 rpm) to remove impurities.
[0075] The separated and purified extract was filtered using a 0.2 μm filter membrane to remove remaining impurities.
[0076] Embodiment 6, select Shenshan Butoutou 7 money, Clematis 20 money, Millettia 9 money, Morus 14 money, Caulis Piperis 18 money, Qiannianjian 7 money, Lycopodiella ciliata 20 money;
[0077] As well as 11 qian of Chuanxiong, 9 qian of Safflower, 7 qian of Qianghuo, 11 qian of Frankincense, 7 qian of Agarwood, 9 qian of Myrrh, 7 qian of Natural Copper, 11 qian of Achyranthes bidentata, 18 qian of Viscum album, 7 qian of Raw Chuanwu, 11 qian of Dipsacus asper, 9 qian of Drynaria fortunei, 15 qian of Duhuo, 11 qian of Arisaema, 4 qian of Scorpion, 9 qian of Panax notoginseng, and 3 qian of clove and basil oil.
[0078] The above medicinal materials were cleaned, dried, inspected and crushed to 80 mesh, and impurities were sieved through a sieve.
[0079] The crushed Chinese medicinal materials are mixed with an extractant (carbon dioxide with a purity of not less than 99.9%) and subjected to supercritical fluid extraction. The working pressure during the extraction process is 10 MPa and the temperature is 35°C.
[0080] The extract was separated and purified by a centrifuge (rotating speed of 3000 rpm) to remove impurities.
[0081] The separated and purified extract was filtered using a 0.2 μm filter membrane to remove remaining impurities.
[0082] Comparative Example 1: 10 qian of Shenshan Butoutou, 13 qian of Millettia reticulata, 17 qian of Morus alba, and 15 qian of Stretching Tendon Grass;
[0083] As well as 15 qian of Chuanxiong, 13 qian of safflower, 13 qian of frankincense, 10 qian of agarwood, 13 qian of myrrh, 10 qian of natural copper, 15 qian of Achyranthes bidentata, 13 qian of mistletoe, 10 qian of raw Chuanwu, 15 qian of Dipsacus asper, 13 qian of Drynaria fortunei, 10 qian of Duhuo, 15 qian of Arisaema, 7 qian of whole scorpion, 13 qian of Panax notoginseng, and 6 qian of clove basil oil.
[0084] The above medicinal materials were cleaned, dried, inspected and crushed to 80 mesh, and impurities were sieved through a sieve.
[0085] The crushed Chinese medicinal materials are mixed with an extractant (carbon dioxide with a purity of not less than 99.9%) and subjected to supercritical fluid extraction. The working pressure during the extraction process is 10 MPa and the temperature is 35°C.
[0086] The extract was separated and purified by a centrifuge (rotating speed of 3000 rpm) to remove impurities.
[0087] The separated and purified extract was filtered using a 0.2 μm filter membrane to remove remaining impurities.
[0088] Comparative Example 2: 10 qian of Shenshan Butoutou, 15 qian of Clematis, 13 qian of Millettia reticulata, 17 qian of Morus alba, 13 qian of Caulis Piperis, 10 qian of Qiannianjian, and 15 qian of Stretching Grass;
[0089] As well as 15 qian of Chuanxiong, 13 qian of Safflower, 10 qian of Qianghuo, 13 qian of Frankincense, 10 qian of Agarwood, 13 qian of Myrrh, 13 qian of Viscum, 10 qian of Raw Chuanwu, 15 qian of Dipsacus asper, 10 qian of Duhuo, 15 qian of Arisaema, 7 qian of Whole Scorpion, 13 qian of Panax notoginseng, and 6 qian of clove and basil oil.
[0090] The above medicinal materials were cleaned, dried, inspected and crushed to 80 mesh, and impurities were sieved through a sieve.
[0091] The crushed Chinese medicinal materials are mixed with an extractant (carbon dioxide with a purity of not less than 99.9%) and subjected to supercritical fluid extraction. The working pressure during the extraction process is 10 MPa and the temperature is 35°C.
[0092] The extract was separated and purified by a centrifuge (rotating speed of 3000 rpm) to remove impurities.
[0093] The separated and purified extract was filtered using a 0.2 μm filter membrane to remove remaining impurities.
[0094] Test Example 1: Comparative analysis was performed on the efficacy of Examples 1 to 6, and Comparative Examples 1 and 2.
[0095] Specifically, 160 patients aged between 35 and 55 years old with different degrees of muscle and bone pain symptoms were selected and divided into 8 groups of 20 people each. The drugs of different proportions and ingredients in Example 1 to Comparative Example 2 were sprayed respectively, with a dosage of 160 ml three times a day for three days. For patients whose symptoms were not relieved, the coating time was extended until relief was achieved, and the action time was evaluated based on pain relief.
[0096]
[0097] From the test results of the above test examples, it can be seen that the drugs provided by Examples 1-6 of the present invention can effectively relieve muscle and bone pain related symptoms, and the pain of most patients will be significantly relieved within half a day after spraying the drugs. In particular, the treatment effects of Examples 2 and 4 are the best, and nearly half of the patients have their pain effectively relieved within one day. Therefore, these embodiments are the best embodiments of the present invention;
[0098] Among them, in Example 4, the proportion of safflower, chuanxiong, Millettia reticulata, agarwood, myrrh, and Panax notoginseng is increased to improve the effects of promoting blood circulation and removing blood stasis, reducing swelling and relieving pain. In the actual use process, the pain relief effect is significantly improved and the action time is relatively shortened.
[0099] Example 6 increases the proportion of Angelica dahurica, Clematis chinensis, Cnidium monnieri, Herba Lycopodii, and Herba Viscum album to improve the effects of warming the meridians, dispelling wind and dampness, and can accelerate the effective relief of muscle and bone pain in patients with rheumatoid arthritis. At the same time, its effects of dispelling wind and dampness can assist in the treatment of rheumatoid arthritis inflammation.
[0100] At the same time, in the comparative example 1, several herbs including Qianghuo, Qiannianjian, Clematis chinensis and Kadzuna were removed. In the actual test process, the effective time for patients with rheumatoid arthritis was significantly prolonged, and the degree of relief was low, and the relative treatment effect was reduced;
[0101] In Comparative Example 2, the ingredients of Achyranthes bidentata, natural copper and Rhizoma Drynariae were eliminated. In the actual test process, the effect time on patients with bone hyperplasia was slow. The pain was relieved after application of the medicine, but obvious pain still existed after the drug effect disappeared. It was diagnosed that the bone repair and regeneration effect was much lower than the rate of the ingredients containing natural copper and Rhizoma Drynariae in Examples 1 to 5.
[0102] The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention. It should be understood by those skilled in the art that the present invention is not limited to the above embodiments. The above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention to be protected. The scope of protection of the present invention is defined by the attached claims and their equivalents.
Claims
1. A method for preparing a drug for treating muscle and bone pain, characterized in that: The method includes: S1. Select the above-mentioned medicinal ingredients that meet the quality standards, fresh and high-quality Chinese herbal medicines, wash, dry, inspect and crush them to make them suitable for extraction; The Chinese herbal medicine includes prescription medicine A and prescription medicine B: The formula medicine A includes: Shen Shan Bu Tu Tou, Clematis chinensis, Millettia spatholobi, Morus alba, Caulis Piperis ternata, Millennium Jian, and Stretching Tendon Grass; The prescription medicine B comprises: Chuanxiong, safflower, Notopterygium wilfordii, frankincense, agarwood, myrrh, natural copper, Achyranthes bidentata, mistletoe, raw Chuanwu, Dipsacus asper, Drynaria officinalis, Angelica dahurica, Arisaema consanguineum, Scorpio, Panax notoginseng, clove basil oil; S2, mixing the crushed Chinese medicinal materials with an extractant and performing supercritical fluid extraction; S3, separating and purifying the extract to remove impurities.
2. A method for preparing a medicine for treating muscle and bone pain according to claim 1, characterized in that: In the pulverizing process described in S2, the medicinal material is pulverized into 50-80 meshes, and the impurities therein are screened out through a sieve.
3. The method for preparing a medicine for treating muscle and bone pain according to claim 1, characterized in that: The extraction agent used in the extraction process in S2 is carbon dioxide, and its purity is not less than 99.9%.
4. A method for preparing a medicine for treating muscle and bone pain according to claim 3, characterized in that: The working pressure of the carbon dioxide during the extraction process is 10-30 MPa, and the temperature of the carbon dioxide during the extraction process is 35-45°C.
5. The method for preparing a medicine for treating muscle and bone pain according to claim 1, characterized in that: 5-15 coins for Deep Mountain, 10-20 coins for Clematis, 8-18 coins for Millettia reticulata, 12-22 coins for Morus alba, 8-18 coins for Caulis Piperis, 5-15 coins for Millennium Health, and 10-20 coins for Smilax glabra.
6. The method for preparing a medicine for treating muscle and bone pain according to claim 1, characterized in that: Ligusticum chuanxiong 10-20 qian, safflower 8-18 qian, angelica dahurica 5-15 qian, frankincense 8-18 qian, agarwood 5-15 qian, myrrh 8-18 qian, natural copper 5-15 qian, Achyranthes bidentata 10-20 qian, mistletoe 8-18 qian, raw Chuanwu 5-15 qian, Dipsacus asper 10-20 qian, Drynaria fortunei 8-18 qian, Duhuo 5-15 qian, Arisaema 10-20 qian, Scorpio 3-12 qian, Panax notoginseng 8-18 qian, clove basil oil 2-10 qian.
7. The method for preparing a medicine for treating muscle and bone pain according to claim 1, characterized in that: In the supercritical fluid extraction process described in S2, the ratio of Chinese medicinal materials to the extractant is 1:5 to 1:
10.
8. The method for preparing a medicine for treating muscle and bone pain according to claim 1, characterized in that: The separation and purification step in S3 is achieved by centrifugation, and the centrifuge speed is 3000-5000 rpm.
9. The method for preparing a medicine for treating muscle and bone pain according to claim 1, characterized in that: The impurity removal process described in S3 is filtered using a 0.2 micron filter membrane.