Pharyngitis adjuvant therapy traditional Chinese medicine formula capable of clearing and nourishing throat and preparation method of pharyngitis adjuvant therapy traditional Chinese medicine formula

Abstract

The invention relates to the technical field of traditional Chinese medicines, and particularly discloses a pharyngitis adjuvant therapy traditional Chinese medicine formula capable of clearing and nourishing throat and a preparation method thereof, and the pharyngitis adjuvant therapy traditional Chinese medicine formula comprises radix pseudostellariae, poria cocos, Chinese yam, radix scrophulariae, honeysuckle, lily, radix rehmanniae, radix rehmanniae preparata, radix ophiopogonis, folium mori, scutellaria baicalensis, platycodon grandiflorum, coptis chinensis, mint, radix saposhnikoviae, dark plum, pericarpium citri reticulatae, scaphium scaphigerum, rhizoma phragmitis and liquorice. According to the invention, a layer-by-layer self-assembly technology is adopted, a slow-release coating with a multi-layer structure is constructed on the surfaces of drug particles, and the coating can control the release speed of the drug, so that the drug is continuously released at the throat part, the action time of the drug is prolonged, and the bioavailability and the treatment effect of the drug are improved; the structure not only enhances the stability of the coating, but also further controls the release speed of the medicine, so that the medicine is released more stably and lastingly.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine formula for auxiliary treatment of pharyngitis capable of clearing the throat and moistening the throat, and a preparation method thereof. Background Art

[0002] Pharyngitis, as a common throat disease, is mainly manifested by symptoms such as sore throat, dry throat, itchy throat, and cough, which brings great inconvenience to patients' lives and work. As a traditional Chinese medicine, Chinese medicine has a long history and profound cultural heritage. Chinese medicine has unique advantages in the treatment of pharyngitis. Its multi-component and multi-target characteristics enable Chinese medicine to comprehensively regulate the various symptoms of pharyngitis with relatively few side effects.

[0003] However, traditional Chinese medicine preparations have problems such as uncontrollable drug release, which affects their therapeutic effects and clinical applications, and there are certain limitations on their use. Summary of the invention

[0004] The object of the present invention is to provide a Chinese medicine formula for adjuvant treatment of pharyngitis which can clear the throat and moisten the throat and a preparation method thereof, so as to solve the problems raised in the above-mentioned background technology.

[0005] To achieve the above object, the present invention provides the following technical solutions:

[0006] A Chinese medicine formula for adjuvant treatment of pharyngitis capable of clearing the throat and moistening the throat, comprising the following Chinese medicine raw materials in parts by weight:

[0007] 9-15 parts of Pseudostellariae Radix, 9-15 parts of Poria, 9-15 parts of Dioscorea opposita, 9-15 parts of Scrophulariae, 6-12 parts of Honeysuckle, 9-12 parts of Lilium, 9-15 parts of Radix Rehmanniae, 9-15 parts of Rehmanniae Preparata, 6-12 parts of Ophiopogon japonicus, 6-9 parts of Morus alba, 6-9 parts of Scutellaria baicalensis, 6-9 parts of Platycodon grandiflorum, 2-5 parts of Coptis chinensis, 3-6 parts of Mentha, 6-9 parts of Saposhnikovia divaricata, 3-6 parts of Plum, 6-9 parts of Tangerine peel, 2-3 parts of Sterculia lychnophora, 9-15 parts of Phragmites australis, 3-6 parts of Licorice, 3-6 parts of Astragalus polysaccharide extract, 0.5-1 parts, 3-5 parts of Ganoderma lucidum spore powder, 6-12 parts of Mulberry seeds, 3-6 parts of Maca powder, and 9-15 parts of Gynostemma pentaphyllum.

[0008] A method for preparing a Chinese medicine formula for auxiliary treatment of pharyngitis capable of clearing the throat and moistening the throat comprises the following steps:

[0009] S1. Rinse all Chinese medicinal materials with running water, put the washed medicinal materials into different containers, add water 6 times the weight of the medicinal materials, soak for 1 hour, then put the rhizome medicinal materials into the pot, add water 8 times the weight of the medicinal materials, boil over high heat, then turn to low heat and simmer for 30 minutes, add leaves, flowers, and fruits of medicinal materials, continue to simmer for 20 minutes, and keep the medicinal liquid in a slightly boiling state during this period;

[0010] S2, filter the decoction through a 100-mesh gauze or filter to remove the residue, pour the filtered liquid into a pot, and slowly concentrate it to 1 / 3 of the original volume over low heat to form a concentrated solution;

[0011] S3, adding astragalus polysaccharide extract, ganoderma lucidum spore powder, mulberry seed powder, maca powder and gynostemma pentaphyllum extract to the concentrated solution and mixing them evenly, and then dispersing them in water to form a suspension;

[0012] S4. Mix the drug suspension with the polycation solution to allow the polycations to be adsorbed on the surface of the drug particles, remove excess polycation solution by centrifugation, wash the particles with deionized water, and then mix the particles with the polyanion solution to allow the polyanions to be adsorbed on the surface of the drug particles that have been adsorbed with the polycations. Repeat the above steps to alternately adsorb the polycations and polyanions to form a multilayer sustained-release coating.

[0013] S5. Dry the self-assembled drug particles layer by layer to remove residual moisture, solidify the coating, add a proper amount of cream base, stir and mix evenly, so that the drug particles are evenly dispersed in the cream base to form a smooth cream, and obtain a Chinese medicine formula for auxiliary treatment of pharyngitis that can clear the throat and moisten the throat.

[0014] Preferably, the polycation is polyallylammonium chloride at a concentration of 1 mg / mL, and the polyanion is sodium polystyrene sulfonate at a concentration of 1 mg / mL.

[0015] Preferably, in S3, the uniformly mixed drug components are dispersed in distilled water, and the suspension is treated with an ultrasonic processor for 30 minutes to form a suspension with a concentration of 10 mg / mL.

[0016] Preferably, in S4, the suspension is mixed with the polycation solution and stirred for 30 minutes to allow the polycation to be adsorbed on the surface of the drug particles, and the excess polycation solution is removed by centrifugation at 3000 rpm for 5 minutes, and the particles are washed twice with 10 mL of distilled water;

[0017] The washed particles were mixed with the polyanion solution and stirred for 30 minutes to allow the polyanions to be adsorbed on the surface of the drug particles that had been adsorbed with the polycations;

[0018] The adsorption of polycations and polyanions was performed alternately, and each layer was washed after adsorption, so that a total of 5 layers of coating were constructed.

[0019] Preferably, in S5, drying is performed in an oven at 40° C. for 4 hours.

[0020] Preferably, in S5, the cream base is one of xanthan gum and carbomer.

[0021] Compared with the prior art, the present invention has the following beneficial effects:

[0022] (1) Through layer-by-layer self-assembly technology, a multi-layer sustained-release coating is constructed on the surface of drug particles. This coating can control the release rate of the drug, so that the drug is continuously released in the throat, prolong the drug action time, and improve the bioavailability and therapeutic effect of the drug. The multi-layer structure is formed by alternating adsorption. This structure not only enhances the stability of the coating, but also further controls the release rate of the drug, making the drug release more stable and lasting.

[0023] (2) Through the combination of multiple Chinese herbal medicines, the synergistic effect of these herbs can comprehensively regulate the uncomfortable symptoms such as sore throat, dry throat, and itchy throat caused by pharyngitis, thereby improving the treatment effect. At the same time, by decocting the rhizome herbs first and then adding the leaves, flowers, and fruits of the herbs, the effective ingredients of each type of herbs can be fully extracted, thereby improving the utilization rate of the drugs. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 It is a flow chart of the present invention. DETAILED DESCRIPTION

[0025] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0026] Embodiment 1:

[0027] See also Figure 1 As shown, a Chinese medicine formula for adjuvant treatment of pharyngitis that can clear the throat and moisten the throat is composed of the following Chinese medicine raw materials in parts by weight:

[0028] 12 parts of Radix Pseudostellariae, 12 parts of Poria, 12 parts of Dioscorea opposita, 12 parts of Scrophulariae, 9 parts of Flos Lonicerae, 10 parts of Lilium, 12 parts of Radix Rehmanniae Preparata, 12 parts of Radix Rehmanniae Preparata, 9 parts of Radix Ophiopogonis, 7 parts of Folium Mori, 7 parts of Radix Scutellariae, 7 parts of Radix Platycodonis, 3 parts of Rhizoma Coptidis, 4 parts of Mentha, 7 parts of Saposhnikoviae, 4 parts of Plum, 7 parts of Pericarpium Citri Reticulatae, 2 parts of Sterculia Lycopodiellae, 12 parts of Rhizoma Phragmites, 4 parts of Licorice, 4 parts of Astragalus Polysaccharide Extract, 4 parts of Ganoderma Lucidum Spore Powder, 9 parts of Fructus Mori, 4 parts of Maca Powder, and 12 parts of Gynostemma Pentaphyllum.

[0029] A method for preparing a Chinese medicine formula for auxiliary treatment of pharyngitis capable of clearing the throat and moistening the throat comprises the following steps:

[0030] S1. Rinse all Chinese medicinal materials with running water, put the washed medicinal materials into different containers, add water 6 times the weight of the medicinal materials, soak for 1 hour, then put the rhizome medicinal materials into the pot, add water 8 times the weight of the medicinal materials, boil over high heat, then turn to low heat and simmer for 30 minutes, add leaves, flowers, and fruits of medicinal materials, continue to simmer for 20 minutes, and keep the medicinal liquid in a slightly boiling state during this period;

[0031] S2, filter the decoction through a 100-mesh gauze or filter to remove the residue, pour the filtered liquid into a pot, and slowly concentrate it to 1 / 3 of the original volume over low heat to form a concentrated solution;

[0032] S3, adding astragalus polysaccharide extract, ganoderma lucidum spore powder, mulberry seed powder, maca powder and gynostemma pentaphyllum extract to the concentrated solution and mixing them evenly, and then dispersing them in water to form a suspension;

[0033] S4. Mix the drug suspension with the polycation solution to allow the polycations to be adsorbed on the surface of the drug particles, remove excess polycation solution by centrifugation, wash the particles with deionized water, and then mix the particles with the polyanion solution to allow the polyanions to be adsorbed on the surface of the drug particles that have been adsorbed with the polycations. Repeat the above steps to alternately adsorb the polycations and polyanions to form a multilayer sustained-release coating.

[0034] S5. Dry the self-assembled drug particles layer by layer to remove residual moisture, solidify the coating, add a proper amount of cream base, stir and mix evenly, so that the drug particles are evenly dispersed in the cream base to form a smooth cream, and obtain a Chinese medicine formula for auxiliary treatment of pharyngitis that can clear the throat and moisten the throat.

[0035] In one embodiment of the present invention, the polycation is polyallylammonium chloride at a concentration of 1 mg / mL, and the polyanion is sodium polystyrene sulfonate at a concentration of 1 mg / mL.

[0036] In one embodiment of the present invention, in S3, the uniformly mixed drug components are dispersed in distilled water, and the suspension is treated with an ultrasonic processor for 30 minutes to form a suspension with a concentration of 10 mg / mL.

[0037] In one embodiment of the present invention, in S4, the suspension is mixed with a polycation solution and stirred for 30 minutes to allow the polycation to be adsorbed on the surface of the drug particles, excess polycation solution is removed by centrifugation at 3000 rpm for 5 minutes, and the particles are washed twice with 10 mL of distilled water;

[0038] The washed particles were mixed with the polyanion solution and stirred for 30 minutes to allow the polyanions to be adsorbed on the surface of the drug particles that had been adsorbed with the polycations;

[0039] The adsorption of polycations and polyanions was performed alternately, and each layer was washed after adsorption, so that a total of 5 layers of coating were constructed.

[0040] In one embodiment of the present invention, in S5, drying is performed in an oven at 40°C for 4 hours.

[0041] In one embodiment of the present invention, in S5, the cream base is one of xanthan gum and carbomer.

[0042] Embodiment 2:

[0043] The difference from Example 1 is that a Chinese medicine formula for adjuvant treatment of pharyngitis that can clear the throat and moisten the throat is composed of the following Chinese medicine raw materials in parts by weight: 9 parts of Pseudostellariae Radix, 15 parts of Poria, 9 parts of Chinese Yam, 15 parts of Scrophulariae Radix, 6 parts of Honeysuckle, 12 parts of Lilium, 9 parts of Radix Rehmanniae, 15 parts of Rehmanniae Preparata, 6 parts of Radix Ophiopogonis, 9 parts of Morus Alpiniae, 6 parts of Scutellariae Baicalensis, 9 parts of Platycodon Grandiflorum, 2 parts of Coptis Chinensis, 6 parts of Mentha, 6 parts of Saposhnikovia Divaricata, 6 parts of Plum, 6 parts of Tangerine Peel, 3 parts of Sterculia lychnophora, 9 parts of Phragmites australis, 6 parts of Licorice, 3 parts of Astragalus polysaccharide extract, 5 parts of Ganoderma lucidum spore powder, 6 parts of Mulberry Fruit, 6 parts of Maca powder, and 9 parts of Gynostemma Pentaphyllum;

[0044] The remaining steps are the same as those in the first embodiment and will not be described in detail again.

[0045] Embodiment three:

[0046] The difference from Example 1 is that a Chinese medicine formula for adjuvant treatment of pharyngitis that can clear the throat and moisten the throat is composed of the following Chinese medicine raw materials in parts by weight: 9 parts of Pseudostellariae Radix, 15 parts of Poria, 9 parts of Chinese Yam, 15 parts of Scrophulariae Radix, 6 parts of Honeysuckle, 12 parts of Lilium, 9 parts of Radix Rehmanniae, 15 parts of Rehmanniae Preparata, 6 parts of Radix Ophiopogonis, 9 parts of Morus Alpiniae, 6 parts of Scutellariae Baicalensis, 9 parts of Platycodon Grandiflorum, 2 parts of Coptis Chinensis, 6 parts of Mentha, 6 parts of Saposhnikovia Divaricata, 6 parts of Plum, 6 parts of Tangerine Peel, 3 parts of Sterculia lychnophora, 9 parts of Phragmites australis, 6 parts of Licorice, 3 parts of Astragalus polysaccharide extract, 5 parts of Ganoderma lucidum spore powder, 6 parts of Mulberry Fruit, 6 parts of Maca powder, and 9 parts of Gynostemma Pentaphyllum;

[0047] The remaining steps are the same as those in the first embodiment and will not be described in detail again.

[0048] Application test case

[0049] 120 adult volunteers with mild to moderate pharyngitis were selected and randomly divided into three groups, with 40 people in each group, and were treated with the Chinese medicine formula prepared in Example 1, Example 2 and Example 3 respectively;

[0050] Take it once in the morning and evening every day, each dose is equivalent to 1 / 20 of the total amount of the original medicinal material, and take it continuously for two weeks.

[0051] The degree of pharyngeal pain (assessed by visual analog scale VAS, 0 points for no pain, 10 points for the most painful), as shown in the following table:

[0052]

[0053] As can be seen above, the average VAS score of pharyngitis before treatment was 6.5±1.2, which dropped to 4.0±1.0 after one week of treatment, and further dropped to 2.5±0.8 after two weeks of treatment;

[0054] Example 2 Group: The average VAS score of pharyngitis before treatment was 6.3±1.3, 4.2±1.1 after one week of treatment, and 2.8±0.9 after two weeks of treatment;

[0055] Example 3 Group: The average VAS score of pharyngitis before treatment was 6.4±1.1, 4.1±1.0 after one week of treatment, and 2.7±0.7 after two weeks of treatment;

[0056] Prior art group: the average VAS score of pharyngitis before treatment was 7.2±0.1, 5.3±0.9 after one week of treatment, and 3.2±0.7 after two weeks of treatment;

[0057] It can be seen from the data that the degree of throat pain in the three embodiment groups was significantly improved after treatment, and the degree of improvement was better than that in the prior art group.

[0058] Improvement of symptoms such as dry throat, itchy throat, and cough (assessed using a symptom scoring table, with each symptom scored 0-3 points based on absence, mild, moderate, or severe), as shown in the following table:

[0059]

[0060] As can be seen above, the average total symptom score before treatment was 9.2±2.1, which dropped to 5.6±1.8 after one week of treatment, and further dropped to 3.1±1.2 after two weeks of treatment;

[0061] Example 2 Group: The average value of the total symptom score before treatment was 9.0±2.0, 5.9±1.7 after one week of treatment, and 3.3±1.3 after two weeks of treatment;

[0062] Example 3 Group: The average value of the total symptom score before treatment was 9.1±2.2, 5.8±1.9 after one week of treatment, and 3.2±1.1 after two weeks of treatment;

[0063] Prior art group: the mean total symptom score before treatment was 9.2±2.1, after one week of treatment it was 6.0±1.5, and after two weeks of treatment it was 3.5±1.5;

[0064] The three embodiment groups also showed significant effects in improving symptoms such as dry throat, itchy throat, and cough, and were superior to the prior art group.

[0065] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A Chinese medicine formula for adjuvant treatment of pharyngitis that can clear the throat and moisten the throat, characterized in that: It is composed of the following Chinese medicinal raw materials in parts by weight: 9-15 parts of Pseudostellariae Radix, 9-15 parts of Poria, 9-15 parts of Dioscorea opposita, 9-15 parts of Scrophulariae, 6-12 parts of Honeysuckle, 9-12 parts of Lilium, 9-15 parts of Radix Rehmanniae, 9-15 parts of Rehmanniae Preparata, 6-12 parts of Ophiopogon japonicus, 6-9 parts of Morus alba, 6-9 parts of Scutellaria baicalensis, 6-9 parts of Platycodon grandiflorum, 2-5 parts of Coptis chinensis, 3-6 parts of Mentha, 6-9 parts of Saposhnikovia divaricata, 3-6 parts of Plum, 6-9 parts of Tangerine peel, 2-3 parts of Sterculia lychnophora, 9-15 parts of Phragmites australis, 3-6 parts of Licorice, 3-6 parts of Astragalus polysaccharide extract, 0.5-1 parts, 3-5 parts of Ganoderma lucidum spore powder, 6-12 parts of Mulberry seeds, 3-6 parts of Maca powder, and 9-15 parts of Gynostemma pentaphyllum.

2. A method for preparing a Chinese medicine formula for adjuvant treatment of pharyngitis that can clear the throat and moisten the throat, characterized in that: The following steps are involved: S1. Rinse all Chinese medicinal materials with running water, put the washed medicinal materials into different containers, add water 6 times the weight of the medicinal materials, soak for 1 hour, then put the rhizome medicinal materials into the pot, add water 8 times the weight of the medicinal materials, boil over high heat, then turn to low heat and simmer for 30 minutes, add leaves, flowers, and fruits of medicinal materials, continue to simmer for 20 minutes, and keep the medicinal liquid in a slightly boiling state during this period; S2, filter the decoction through a 100-mesh gauze or filter to remove the residue, pour the filtered liquid into a pot, and slowly concentrate it to 1 / 3 of the original volume over low heat to form a concentrated solution; S3, adding astragalus polysaccharide extract, ganoderma lucidum spore powder, mulberry seed powder, maca powder and gynostemma pentaphyllum extract to the concentrated solution and mixing them evenly, and then dispersing them in water to form a suspension; S4. Mix the drug suspension with the polycation solution to allow the polycations to be adsorbed on the surface of the drug particles, remove excess polycation solution by centrifugation, wash the particles with deionized water, and then mix the particles with the polyanion solution to allow the polyanions to be adsorbed on the surface of the drug particles that have been adsorbed with the polycations. Repeat the above steps to alternately adsorb the polycations and polyanions to form a multilayer sustained-release coating. S5. Dry the self-assembled drug particles layer by layer to remove residual moisture, solidify the coating, add a proper amount of cream base, stir and mix evenly, so that the drug particles are evenly dispersed in the cream base to form a smooth cream, and obtain a Chinese medicine formula for auxiliary treatment of pharyngitis that can clear the throat and moisten the throat.

3. The method for preparing a Chinese medicine formula for adjuvant treatment of pharyngitis capable of clearing the throat and moistening the throat according to claim 2, characterized in that: The polycation is polyallylammonium chloride with a concentration of 1 mg / mL, and the polyanion is sodium polystyrene sulfonate with a concentration of 1 mg / mL.

4. The method for preparing a Chinese medicine formula for adjuvant treatment of pharyngitis capable of clearing the throat and moistening the throat according to claim 1, characterized in that: In the S3, the uniformly mixed drug components are dispersed in distilled water, and the suspension is treated with an ultrasonic processor for 30 minutes to form a suspension with a concentration of 10 mg / mL.

5. The method for preparing a Chinese medicine formula for adjuvant treatment of pharyngitis capable of clearing the throat and moistening the throat according to claim 1, characterized in that: In the S4, the suspension is mixed with the polycation solution and stirred for 30 minutes to allow the polycation to be adsorbed on the surface of the drug particles, and the excess polycation solution is removed by centrifugation at 3000 rpm for 5 minutes, and the particles are washed twice with 10 mL of distilled water; The washed particles were mixed with the polyanion solution and stirred for 30 minutes to allow the polyanions to be adsorbed on the surface of the drug particles that had been adsorbed with the polycations; The adsorption of polycations and polyanions was performed alternately, and each layer was washed after adsorption, so that a total of 5 layers of coating were constructed.

6. The method for preparing a Chinese medicine formula for adjuvant treatment of pharyngitis capable of clearing the throat and moistening the throat according to claim 1, characterized in that: In the S5, drying is performed at 40° C. for 4 hours using an oven.

7. The method for preparing a Chinese medicine formula for adjuvant treatment of pharyngitis capable of clearing the throat and moistening the throat according to claim 1, characterized in that: In said S5, the cream base is one of xanthan gum and carbomer.

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