Emotion relieving capsule preparation method based on compound formula

Abstract

The invention relates to the technical field of pet medicine research and development, and particularly discloses an emotion relieving capsule preparation method based on a composite formula, the method comprises the following steps: mixing lavender extract and gamma-cyclodextrin to obtain embedding modified micro powder, carrying out enzymolysis on L-theanine to prepare powder, cross-linking gamma-aminobutyric acid and sodium alginate to obtain a nano composite carrier, and carrying out freeze drying on the nano composite carrier to obtain the emotion relieving capsule based on the composite formula. Mixing the three components to prepare a slow-release composite base material, mixing the slow-release composite base material with vitamin B6 and the like, sieving, finally filling into a hydroxypropyl methylcellulose hollow capsule shell, and coating with a film to prepare the capsule. Parameters such as raw material proportion and reaction conditions are definitely limited in each step. According to the capsule, the emotion of pets is relieved through a compound formula and cooperation of multiple components, the preparation method is scientific and reasonable, the product quality is controllable, the emotion problem caused by factors such as environment and living habits of the pets can be effectively solved, and the capsule has good application prospects.

Description

Technical Field

[0001] The present invention relates to the technical field of pet medicine research and development, and specifically to a preparation method of an emotion soothing capsule based on a compound formula. Background Art

[0002] In modern society, pets play an increasingly important role in people's lives and become intimate partners of many families. However, pets face various factors that may cause mood swings in their daily lives, which seriously affect the physical and mental health and quality of life of pets, and also bring troubles to pet owners.

[0003] The environmental factors where pets are located are one of the important reasons for triggering emotional problems. For example, the living space of modern families is relatively limited, and the activity range of pets is restricted. Living in such an environment for a long time, pets are prone to a sense of depression. At the same time, the frequent changes in the indoor and outdoor environments, such as temperature, humidity, noise, etc., will also stimulate the emotions of pets. For example, the high temperature in summer may make pets fidgety; while the frequent vehicle honking and construction noise in the city will make pets feel nervous and frightened.

[0004] The change of pets' living habits often triggers emotional problems. When pet owners change their jobs, move, etc., resulting in changes in the diet and rest time of pets, it is very difficult for pets to adapt in a short time. For example, if the original regular feeding time is suddenly changed, or if the pet is moved from a familiar living environment to a strange place, the pet may have emotional reactions such as anxiety and depression, manifested as loss of appetite, excessive licking of hair, abnormal vocalization and other behaviors.

[0005] In addition, the change in the interaction mode between pets and their owners will also affect their emotional state. The modern life rhythm is fast, and the time that pet owners accompany their pets is reduced. Pets lack sufficient attention and company, and are prone to loneliness. Moreover, improper training methods for pets, such as being overly strict or using violent means, will leave psychological shadows on pets, resulting in unstable emotions and even aggression.

[0006] At present, there are many deficiencies in the solutions to pet emotional problems. Some pet owners choose to change the living environment of pets to relieve their emotional problems, such as adding pet toys, expanding the activity space, etc., but this method often only plays a short-term soothing role and cannot fundamentally solve the problem. There are also some owners who adopt soothing behaviors, such as stroking, accompanying, etc., but for pets with more serious emotional problems, these methods have limited effects.

[0007] In terms of drug intervention, most of the existing drugs for pet emotional problems are simple sedatives. Although these drugs can relieve the emotional symptoms of pets to a certain extent, they have obvious side effects. Long-term use may affect the normal physiological functions of pets, such as causing pets to be lethargic, listless, and loss of appetite. It may also lead to drug dependence, posing a potential threat to the health of pets.

[0008] There are also many defects in the existing pet emotion regulation products on the market. The formula of some products is single, only targeting a certain emotional problem for regulation, and unable to comprehensively improve the complex emotional state of pets. Moreover, the dosage form design of these products is unreasonable. For example, the taste of some oral products is not good, and pets resist taking them, affecting the use effect. In terms of product quality, due to the lack of strict supervision and unified standards, the quality of pet emotion regulation products on the market varies, and it is difficult to ensure their safety and effectiveness.

[0009] Therefore, it is of great practical significance to develop a safe, effective, scientifically formulated and highly targeted pet emotion soothing product. A method for preparing an emotion soothing capsule based on a composite formula of the present invention aims to solve the deficiencies of existing pet emotion regulation means and provide more reliable protection for the mental health of pets. Summary of the Invention

[0010] (I) Technical problems to be solved In view of the deficiencies of the prior art, the present invention provides a method for preparing an emotion soothing capsule based on a composite formula, which solves the problems set forth in the above background art.

[0011] (II) Technical solutions In order to achieve the above object, the present invention discloses a method for preparing an emotion soothing capsule based on a composite formula, comprising the following steps: Step 1: Mix lavender extract and γ-cyclodextrin in proportion, add deionized water, ultrasonically disperse for 30 min, stir at a constant temperature of 40 - 50 °C for 4 - 6 h, vacuum filter and then freeze-dry to obtain embedded modified lavender micropowder; Step 2: Dissolve L-theanine in a citric acid buffer solution, adjust the pH to 5.5 - 6.0, add horseradish peroxidase, react at 35 - 40 °C for 2 - 3 h, inactivate the enzyme and then dialyze and purify, and spray-dry to obtain enzymatically hydrolyzed theanine powder; Step 3: Mix γ-aminobutyric acid and sodium alginate by mass ratio, dissolve in a phosphate buffer solution, add calcium chloride solution for crosslinking, centrifuge to collect gel particles, vacuum dry and then pulverize into a nano-composite carrier; Step 4: Disperse the embedded modified lavender micropowder, enzymatically hydrolyzed theanine powder, and the nano-composite carrier in polyethylene glycol 400 in proportion, perform ultrasonic homogenization treatment for 20 min, and evaporate the solvent under reduced pressure at 50 - 60 °C to obtain a sustained-release composite base material; Step 5: Mix the sustained-release composite base material with vitamin B6, magnesium whey protein hydrolysate, and microcrystalline cellulose, use a three-dimensional motion mixer to mix at a rotation speed of 25 r / min for 40 min, and pass through an 80-mesh sieve; Step 6: Fill the mixed powder into hydroxypropyl methylcellulose hollow capsule shells, seal in an environment with humidity ≤ 30%, and spray Opadry film coating using a fluidized bed coater, controlling the inlet air temperature at 45 - 50 °C to obtain mood-soothing capsules.

[0012] Preferably, in Step 1, the mass ratio of lavender extract, γ-cyclodextrin, and deionized water is 1:3 - 5:50 - 80, and the freeze-drying conditions are pre-freezing at -40 °C for 24 h and drying under a vacuum of ≤ 10 Pa for 48 h.

[0013] Preferably, in Step 2, the mass-to-volume ratio of L-theanine, citrate buffer, and horseradish peroxidase is 100 g:500 - 800 mL:0.5 - 1.2 U / g, and dialysis purification is performed using a dialysis bag with a molecular weight cut-off of 3 kDa.

[0014] Preferably, in Step 3, the mass ratio of γ-aminobutyric acid to sodium alginate is 1:2 - 4, the phosphate buffer concentration is 0.1 M pH 7.4, the calcium chloride solution concentration is 2 - 4% w / v, and the crosslinking time is 30 - 45 min.

[0015] Preferably, in Step 4, the mass ratio of the embedded modified lavender micropowder, enzymatically hydrolyzed theanine powder, and the nano-composite carrier is 5 - 8:3 - 6:1 - 2, and the addition amount of polyethylene glycol 400 is 150 - 200% of the total solid mass.

[0016] Preferably, in Step 5, the mass ratio of the sustained-release composite base material, vitamin B6, magnesium whey protein hydrolysate, and microcrystalline cellulose is 60 - 75:5 - 8:10 - 15:10 - 20, and the working air pressure of the three-dimensional mixer is 0.4 - 0.6 MPa.

[0017] Preferably, in Step 6, the hydroxypropyl methylcellulose content of the hollow capsule shell is 18 - 22%, the film coating weight gain rate is 3 - 5%, and the solid content of the coating solution is 8 - 12%.

[0018] Preferably, in Step 5, the angle of repose of the mixed powder is ≤ 30°, the bulk density is 0.45 - 0.55 g / cm³, and the moisture content is ≤ 3.0%.

[0019] Preferably, in step six, the atomization pressure of the fluidized bed coater is 0.8 - 1.2 bar, the liquid spraying rate is 6 - 10 mL / min, and the coating thickness is 20 - 30 μm.

[0020] Preferably, in step two, the enzyme inactivation treatment is carried out by heating in a boiling water bath for 10 min, the inlet air temperature of spray drying is 160 - 180 °C, and the outlet air temperature is 75 - 85 °C.

[0021] (III) Beneficial technical effects Lavender extract has natural sedative and nerve-soothing effects, and its unique volatile aroma can effectively relieve the tension and anxiety of pets. After γ-cyclodextrin entraps and modifies lavender extract, it not only improves the stability of lavender extract, but also enables its slow release and prolongs the action time. After L-theanine is enzymatically hydrolyzed, it is more easily absorbed by the pet's body. It can cross the blood-brain barrier, act on brain neurotransmitters, inhibit the overexcitement of the nervous system, and cooperate with lavender extract to further enhance the mood-soothing effect. As an important neurotransmitter inhibitor, γ-aminobutyric acid can effectively reduce the stress response of pets, improve adverse emotions such as anxiety and depression, and act together with other components to comprehensively regulate the mood state of pets.

[0022] During the preparation process, by crosslinking γ-aminobutyric acid with sodium alginate to form a nano-composite carrier, a stable carrier environment is provided for the active ingredients. This nano-composite carrier, entrapped and modified lavender micropowder, and enzymatically hydrolyzed theanine powder are dispersed in polyethylene glycol 400 and made into a sustained-release composite base material, enabling the capsule to slowly release the active ingredients in the pet's gastrointestinal tract. On the one hand, it ensures that the active ingredients play a role in the body for a long time and reduces the frequency of taking medicine; on the other hand, it avoids excessive release of too many ingredients at one time from irritating the pet's gastrointestinal tract, improving the safety and effectiveness of the product.

[0023] The capsule dosage form is paired with a hypromellose hollow capsule shell, which is tasteless and has little irritation to the pet's gastrointestinal tract. At the same time, an Opadry film coating is sprayed using a fluidized bed coater, which can not only cover up the bad taste of the contents, but also improve the appearance and taste of the capsule. Compared with some oral products on the market with poor taste and pets' resistance to taking them, the mood-soothing capsule of the present invention is more easily accepted by pets, improves the compliance of pets taking medicine, ensures that pets can take the active ingredients on time and in the right amount, and thus better plays the role of regulating mood.

[0024] The components selected in the present invention are all natural extracts or biodegradable materials, such as lavender extract, L-theanine, γ-aminobutyric acid, sodium alginate, etc. While effectively regulating the emotions of pets, it greatly reduces the potential harm to the bodies of pets. Compared with some existing simple sedative drugs, this mood-soothing capsule will not cause adverse reactions such as drowsiness, listlessness, and loss of appetite in pets, nor does it have the problem of drug dependence, and has no adverse effect on the normal physiological functions of pets, providing a reliable guarantee for the long-term health of pets.

[0025] Some pet mood regulation products on the market have a single formula and are difficult to comprehensively improve the complex mood problems of pets. However, through the synergistic effect of multiple components, the mood-soothing capsule of the present invention can regulate a variety of mood problems of pets, such as anxiety, depression, tension, fear, etc. Whether it is due to environmental changes, changes in living habits, or reduced interaction with the owner, it can achieve good improvement effects and comprehensively improve the mental health level and quality of life of pets.

[0026] During the preparation process, the parameters of each link are strictly controlled, such as the proportion of each component, reaction temperature, time, drying conditions, etc. For example, precisely controlling the lyophilization conditions in step one, the enzymatic hydrolysis reaction parameters in step two, the cross-linking conditions in step three, etc., to ensure that the quality of each batch of products is stable and consistent. At the same time, strict control of indicators such as the hydroxypropyl methylcellulose content of the hollow capsule shell, the film coating weight gain rate, and the solid content of the coating solution, as well as the standardization of physical properties such as the angle of repose, bulk density, and moisture content of the mixed powder, make the product quality reliable, and the safety and effectiveness are fully guaranteed, reducing the risk to the health of pets caused by product quality problems. Detailed implementation methods

[0027] To facilitate the understanding of the present invention, the present invention will be described more comprehensively below. The following gives the preferred embodiments of the present invention. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the understanding of the disclosure content of the present invention more thorough and comprehensive.

[0028] Example 1: A preparation method of a mood-soothing capsule based on a composite formula, comprising the following steps: Step one: Mix lavender extract and γ-cyclodextrin in a mass ratio of 1:3, add 50 times the mass of deionized water, ultrasonically disperse for 30 min, stir at a constant temperature of 40 °C for 4 h, vacuum filter, and then pre-freeze at -40 °C for 24 h, and dry under the condition of a vacuum degree ≤ 10 Pa for 48 h to obtain embedded and modified lavender micropowder; Step 2: Dissolve 100 g of L-theanine in 500 mL of citrate buffer, adjust the pH to 5.5, add horseradish peroxidase at 0.5 U / g, react at 35 °C for 2 h, inactivate the enzyme by heating in a boiling water bath for 10 min, purify by dialysis using a dialysis bag with a molecular weight cut-off of 3 kDa, and spray-dry under the conditions of an inlet air temperature of 160 °C and an outlet air temperature of 75 °C to obtain enzymatically hydrolyzed theanine powder; Step 3: Mix γ-aminobutyric acid and sodium alginate at a mass ratio of 1:2, dissolve them in 0.1 M phosphate buffer at pH 7.4, add a calcium chloride solution with a concentration of 2% w / v for crosslinking for 30 min, centrifuge to collect gel particles, and pulverize them into nano-composite carriers after vacuum drying; Step 4: Disperse the embedded and modified lavender micropowder, enzymatically hydrolyzed theanine powder, and nano-composite carrier in polyethylene glycol 400 at a mass ratio of 5:3:1. The addition amount of polyethylene glycol 400 is 150% of the total solid mass. Perform ultrasonic homogenization for 20 min, and evaporate the solvent under reduced pressure at 50 °C to obtain a sustained-release composite base material; Step 5: Mix the sustained-release composite base material, vitamin B6, magnesium whey protein hydrolyzate, and microcrystalline cellulose at a mass ratio of 60:5:10:10. Use a three-dimensional motion mixer to mix at a rotation speed of 25 r / min and a working air pressure of 0.4 MPa for 40 min, pass through an 80-mesh sieve. The angle of repose of the mixed powder is ≤30°, the bulk density is 0.45 g / cm³, and the moisture content is ≤3.0%; Step 6: Fill the mixed powder into hydroxypropyl methylcellulose hollow capsule shells. The hydroxypropyl methylcellulose content of the hollow capsule shells is 18%. Seal in an environment with a humidity ≤30%. Spray Opadry film coating using a fluidized bed coater, control the inlet air temperature at 45 °C, the atomization pressure at 0.8 bar, the liquid spraying rate at 6 mL / min, the coating thickness at 20 μm, and the film coating weight gain rate at 3% to obtain the mood-soothing capsules.

[0029] Example 2: A method for preparing mood-soothing capsules based on a composite formula, comprising the following steps: Step 1: Mix lavender extract and γ-cyclodextrin at a mass ratio of 1:4, add 65 times the mass of deionized water, ultrasonically disperse for 30 min, stir at a constant temperature of 45 °C for 5 h, perform vacuum filtration, and then place it at -40 °C for pre-freezing for 24 h, and dry under the condition of a vacuum degree ≤10 Pa for 48 h to obtain embedded and modified lavender micropowder; Step 2: Dissolve 100 g of L-theanine in 650 mL of citric acid buffer solution, adjust the pH to 5.8, add horseradish peroxidase at 0.8 U / g, react at 38 °C for 2.5 h, inactivate the enzyme by heating in a boiling water bath for 10 min, purify by dialysis using a dialysis bag with a molecular weight cut-off of 3 kDa, and spray-dry under the conditions of an inlet air temperature of 170 °C and an outlet air temperature of 80 °C to obtain enzymatically hydrolyzed theanine powder; Step 3: Mix γ-aminobutyric acid and sodium alginate in a mass ratio of 1:3, dissolve in 0.1 M phosphate buffer solution with pH 7.4, add calcium chloride solution with a concentration of 3% w / v for cross-linking for 38 min, centrifuge to collect gel particles, and pulverize them into nano-composite carriers after vacuum drying; Step 4: Disperse the embedded modified lavender micropowder, enzymatically hydrolyzed theanine powder, and nano-composite carrier in polyethylene glycol 400 in a mass ratio of 6:4:1.5. The addition amount of polyethylene glycol 400 is 175% of the total solid mass. Perform ultrasonic homogenization for 20 min, and evaporate the solvent under reduced pressure at 55 °C to obtain a sustained-release composite base material; Step 5: Mix the sustained-release composite base material, vitamin B6, magnesium whey protein hydrolysate, and microcrystalline cellulose in a mass ratio of 68:6:12:15. Use a three-dimensional motion mixer to mix at a rotation speed of 25 r / min and a working air pressure of 0.5 MPa for 40 min, pass through an 80-mesh sieve. The angle of repose of the mixed powder is ≤30°, the bulk density is 0.50 g / cm³, and the moisture content is ≤3.0%; Step 6: Fill the mixed powder into hydroxypropyl methylcellulose hollow capsule shells. The hydroxypropyl methylcellulose content of the hollow capsule shells is 20%. Seal in an environment with a humidity ≤30%. Spray Opadry film coating using a fluidized bed coater, control the inlet air temperature at 48 °C, the atomization pressure at 1.0 bar, the liquid spraying rate at 8 mL / min, the coating thickness at 25 μm, and the film coating weight gain rate at 4% to obtain the mood-soothing capsules.

[0030] Example 3: A method for preparing mood-soothing capsules based on a composite formula, comprising the following steps: Step 1: Mix lavender extract and γ-cyclodextrin in a mass ratio of 1:5, add 80 times the mass of deionized water, perform ultrasonic dispersion for 30 min, stir at a constant temperature of 50 °C for 6 h, perform vacuum filtration, and then place in a -40 °C freezer for pre-freezing for 24 h, and dry under the condition of a vacuum degree ≤10 Pa for 48 h to obtain embedded modified lavender micropowder; Step 2: Dissolve 100 g of L-theanine in 800 mL of citrate buffer solution, adjust the pH to 6.0, add horseradish peroxidase at a dosage of 1.2 U / g, react at 40 °C for 3 h, inactivate the enzyme by heating in a boiling water bath for 10 min, purify by dialysis using a dialysis bag with a molecular weight cut-off of 3 kDa, and spray-dry under the conditions of an inlet air temperature of 180 °C and an outlet air temperature of 85 °C to obtain enzymatically hydrolyzed theanine powder; Step 3: Mix γ-aminobutyric acid and sodium alginate at a mass ratio of 1:4, dissolve them in 0.1 M phosphate buffer solution with a pH of 7.4, add a calcium chloride solution with a concentration of 4% w / v for crosslinking for 45 min, centrifuge to collect gel particles, and pulverize them into nano-composite carriers after vacuum drying; Step 4: Disperse the embedded and modified lavender micropowder, enzymatically hydrolyzed theanine powder, and nano-composite carrier at a mass ratio of 8:6:2 in polyethylene glycol 400. The addition amount of polyethylene glycol 400 is 200% of the total solid mass, perform ultrasonic homogenization treatment for 20 min, and evaporate the solvent under reduced pressure at 60 °C to obtain a sustained-release composite base material; Step 5: Mix the sustained-release composite base material, vitamin B6, magnesium whey protein hydrolysate, and microcrystalline cellulose at a mass ratio of 75:8:15:20, mix them using a three-dimensional motion mixer at a rotation speed of 25 r / min and a working air pressure of 0.6 MPa for 40 min, pass through an 80-mesh sieve. The angle of repose of the mixed powder is ≤30°, the bulk density is 0.55 g / cm³, and the moisture content is ≤3.0%; Step 6: Fill the mixed powder into hydroxypropylmethylcellulose empty capsule shells. The hydroxypropylmethylcellulose content of the empty capsule shells is 22%, seal them in an environment with a humidity ≤30%, spray Opadry film coating using a fluidized bed coater, control the inlet air temperature at 50 °C, the atomization pressure at 1.2 bar, the liquid spraying rate at 10 mL / min, the coating thickness at 30 μm, and the film coating weight gain rate at 5% to obtain the mood-soothing capsules.

[0031] Comparative Example 1: A preparation method of a similar product, similar to Example 2, but γ-cyclodextrin was not used to encapsulate the lavender extract in Step 1. The lavender extract was directly mixed and stirred with deionized water and dried, and the other steps were the same as in Example 2.

[0032] Step 1: Add the lavender extract to 65 times its mass of deionized water, stir for 4 h, and dry at 45 °C to obtain unencapsulated lavender powder; Step 2: Dissolve 100 g of L-theanine in 650 mL of citric acid buffer solution, adjust the pH to 5.8, add horseradish peroxidase at 0.8 U / g, react at 38 °C for 2.5 h, inactivate the enzyme by heating in a boiling water bath for 10 min, purify by dialysis using a dialysis bag with a molecular weight cut-off of 3 kDa, and spray-dry under the conditions of an inlet air temperature of 170 °C and an outlet air temperature of 80 °C to obtain enzymatically hydrolyzed theanine powder; Step 3: Mix γ-aminobutyric acid and sodium alginate at a mass ratio of 1:3, dissolve them in 0.1 M phosphate buffer solution at pH 7.4, add calcium chloride solution with a concentration of 3% w / v for cross-linking for 38 min, centrifuge to collect gel particles, and pulverize them into nano-composite carriers after vacuum drying; Step 4: Disperse unembedded lavender powder, enzymatically hydrolyzed theanine powder and nano-composite carriers at a mass ratio of 6:4:1.5 in polyethylene glycol 400. The addition amount of polyethylene glycol 400 is 175% of the total solid mass, perform ultrasonic homogenization treatment for 20 min, and evaporate the solvent under reduced pressure at 55 °C to obtain a composite base material; Step 5: Mix the composite base material, vitamin B6, magnesium whey protein hydrolysate and microcrystalline cellulose at a mass ratio of 68:6:12:15, use a three-dimensional motion mixer to mix at a rotation speed of 25 r / min and a working air pressure of 0.5 MPa for 40 min, pass through an 80-mesh sieve. The angle of repose of the mixed powder is ≤30°, the bulk density is 0.50 g / cm³, and the moisture content is ≤3.0%; Step 6: Fill the mixed powder into hydroxypropyl methylcellulose hollow capsule shells. The hydroxypropyl methylcellulose content of the hollow capsule shells is 20%, seal in an environment with a humidity ≤30%, spray Opadry film coating using a fluidized bed coater, control the inlet air temperature at 48 °C, the atomization pressure at 1.0 bar, the liquid spraying rate at 8 mL / min, the coating thickness at 25 μm, and the film coating weight gain rate at 4% to obtain a similar product.

[0033] Comparative Example 2: A preparation method of a similar product, similar to Example 2, but in Step 2, no enzymatic hydrolysis reaction is carried out, directly dissolve L-theanine and spray-dry it, and other steps are the same as in Example 2.

[0034] Step 1: Mix lavender extract and γ-cyclodextrin at a mass ratio of 1:4, add 65 times the mass of deionized water, ultrasonically disperse for 30 min, stir at a constant temperature of 45 °C for 5 h, vacuum filter and then place in a freezer at -40 °C for pre-freezing for 24 h, and dry under the condition of a vacuum degree ≤10 Pa for 48 h to obtain embedded and modified lavender micropowder; Step 2: Dissolve 100 g of L-theanine in 650 mL of citric acid buffer solution, adjust the pH to 5.8, and directly spray-dry it under the conditions of an inlet air temperature of 170 °C and an outlet air temperature of 80 °C to obtain L-theanine powder; Step 3: Mix γ-aminobutyric acid and sodium alginate at a mass ratio of 1:3, dissolve them in 0.1 M phosphate buffer solution with pH 7.4, add a calcium chloride solution with a concentration of 3% w / v for crosslinking for 38 min, centrifuge to collect gel particles, and pulverize them into nano-composite carriers after vacuum drying; Step 4: Disperse the embedded modified lavender micropowder, L-theanine powder and nano-composite carrier at a mass ratio of 6:4:1.5 in polyethylene glycol 400. The addition amount of polyethylene glycol 400 is 175% of the total solid mass, perform ultrasonic homogenization treatment for 20 min, and evaporate the solvent under reduced pressure at 55 °C to obtain a composite base material; Step 5: Mix the composite base material, vitamin B6, magnesium whey protein hydrolyzate and microcrystalline cellulose at a mass ratio of 68:6:12:15, use a three-dimensional motion mixer to mix at a rotation speed of 25 r / min and a working air pressure of 0.5 MPa for 40 min, pass through an 80-mesh sieve, the angle of repose of the mixed powder ≤ 30°, the bulk density is 0.50 g / cm³, and the moisture content ≤ 3.0%; Step 6: Fill the mixed powder into hydroxypropylmethylcellulose hollow capsule shells. The hydroxypropylmethylcellulose content of the hollow capsule shells is 20%, seal them in an environment with humidity ≤ 30%, spray Opadry film coating with a fluidized bed coater, control the inlet air temperature at 48 °C, the atomization pressure at 1.0 bar, the liquid spraying rate at 8 mL / min, the coating thickness at 25 μm, and the film coating weight gain rate at 4% to obtain a similar product.

[0035] Test the mood-soothing capsules and similar products prepared in Examples 1-3 and Comparative Examples 1-2, and the test results are as follows: (1) Stability test: Place the samples in an environment of high temperature (40 °C) and high humidity (75% RH) for 3 months, observe whether there are changes in appearance, and measure the changes in the content of active ingredients. The results are shown in Table 1.

[0036] Table 1: Test Items Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Appearance Change No Obvious Change No Obvious Change No Obvious Change Slight Deformation of Capsule Shell and Caking of Contents Slight Discoloration of Capsule Shell and Agglomeration of Contents Rate of Change in Lavender Extract Content (%) 5.2 4.5 4.0 18.6 15.3 Rate of Change in L-Theanine Content (%) 6.1 5.3 4.8 12.5 10.7 Rate of Change in γ-Aminobutyric Acid Content (%) 5.8 5.0 4.3 14.2 11.8 According to the test results in Table 1, it can be seen that the mood-soothing capsules corresponding to Examples 1-3 have good stability and small changes in the content of active ingredients. In Comparative Example 1, the lavender extract was not embedded, resulting in a significant decrease in its stability; in Comparative Example 2, L-theanine was not enzymatically hydrolyzed, which also affected the product stability to a certain extent.

[0037] (2) Soothing effect test: Select 50 healthy but stress-prone pet cats and randomly divide them into 5 groups, with 10 cats in each group. Administer the emotional soothing capsules of Examples 1-3 and the similar products of Comparative Examples 1-2 respectively, and continuously feed them at a dose of 0.1 g per kilogram of body weight for 14 days. Before and after feeding, use the behavioral scoring method to evaluate the emotional state of the pets. The scoring criteria are as follows: 0-2 points indicate stable emotions, 3-5 points indicate mild stress, 6-8 points indicate moderate stress, and 9-10 points indicate severe stress. The results are shown in Table 2.

[0038] Table 2:

[0039] It can be seen from the test results in Table 2 that the emotional soothing capsules corresponding to Examples 1-3 have a significant emotional soothing effect on pets, and the score reduction range is relatively large. The soothing effects of the similar products of Comparative Example 1 and Comparative Example 2 are relatively poor, indicating that the composite formula and preparation method of the present invention have an important impact on the soothing effect of the product.

[0040] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the present invention. All equivalent changes and improvements made in accordance with the scope of the present invention application shall still fall within the scope covered by the patent of the present invention.

Claims

1. A method for preparing an emotion-relieving capsule based on a composite formula, characterized in that, It includes the following steps: Step 1: Mix lavender extract and γ-cyclodextrin in a certain proportion, add deionized water, ultrasonically disperse for 30 min, stir at a constant temperature of 40 - 50 °C for 4 - 6 h, perform vacuum filtration and then freeze-dry to obtain encapsulated and modified lavender micropowder; Step 2: Dissolve L-theanine in a citric acid buffer solution, adjust the pH to 5.5 - 6.0, add horseradish peroxidase, react at 35 - 40 °C for 2 - 3 h, inactivate the enzyme and then perform dialysis purification, and spray-dry to obtain enzymatically hydrolyzed theanine powder; Step 3: Mix γ-aminobutyric acid and sodium alginate according to the mass ratio, dissolve in a phosphate buffer solution, add a calcium chloride solution for crosslinking, centrifuge to collect gel particles, and vacuum-dry and then pulverize to obtain a nano-composite carrier; Step 4: Disperse the encapsulated and modified lavender micropowder, enzymatically hydrolyzed theanine powder and nano-composite carrier in polyethylene glycol 400 in a certain proportion, perform ultrasonic homogenization for 20 min, and evaporate the solvent under reduced pressure at 50 - 60 °C to obtain a sustained-release composite base material; Step 5: Mix the sustained-release composite base material, vitamin B6, magnesium whey protein hydrolysate and microcrystalline cellulose, use a three-dimensional motion mixer to mix at a rotation speed of 25 r / min for 40 min, and pass through an 80-mesh sieve; Step 6: Fill the mixed powder into hydroxypropyl methylcellulose hollow capsule shells, seal in an environment with humidity ≤ 30%, and use a fluidized bed coater to spray Opadry film coating, controlling the inlet air temperature at 45 - 50 °C to obtain mood-soothing capsules.

2. The preparation method of an emotion-relieving capsule based on a composite formula according to claim 1, characterized in that, In Step 1, the mass ratio of lavender extract, γ-cyclodextrin and deionized water is 1:3 - 5:50 - 80, and the freeze-drying conditions are pre-freezing at -40 °C for 24 h and drying at a vacuum degree ≤ 10 Pa for 48 h.

3. The preparation method of an emotion-relieving capsule based on a composite formula according to claim 1, characterized in that, In Step 2, the mass-volume ratio of L-theanine, citric acid buffer solution and horseradish peroxidase is 100 g:500 - 800 mL:0.5 - 1.2 U / g, and dialysis purification is carried out using a dialysis bag with a cut-off molecular weight of 3 kDa.

4. The preparation method of an emotion-relieving capsule based on a composite formula according to claim 1, wherein, In Step 3, the mass ratio of γ-aminobutyric acid and sodium alginate is 1:2 - 4, the concentration of the phosphate buffer solution is 0.1 M pH 7.4, the concentration of the calcium chloride solution is 2 - 4% w / v, and the crosslinking time is 30 - 45 min.

5. The preparation method of an emotion-relieving capsule based on a composite formula according to claim 1, characterized in that, In Step 4, the mass ratio of the encapsulated and modified lavender micropowder, enzymatically hydrolyzed theanine powder and nano-composite carrier is 5 - 8:3 - 6:1 - 2, and the addition amount of polyethylene glycol 400 is 150 - 200% of the total solid mass.

6. The preparation method of an emotion-relieving capsule based on a composite formula according to claim 1, characterized in that, In Step 5, the mass ratio of the sustained-release composite base material, vitamin B6, magnesium whey protein hydrolysate and microcrystalline cellulose is 60 - 75:5 - 8:10 - 15:10 - 20, and the working air pressure of the three-dimensional mixer is 0.4 - 0.6 MPa.

7. A method for preparing an emotion-relieving capsule based on a composite formula according to claim 1, characterized in that, In Step 6, the hydroxypropyl methylcellulose content of the hollow capsule shell is 18 - 22%, the film coating weight gain rate is 3 - 5%, and the solid content of the coating solution is 8 - 12%.

8. A method for preparing an emotion-relieving capsule based on a composite formula according to claim 6, characterized in that, In Step 5, the angle of repose of the mixed powder ≤ 30°, the bulk density is 0.45 - 0.55 g / cm³, and the moisture content ≤ 3.0%.

9. A method for preparing an emotion-relieving capsule based on a composite formula according to claim 7, characterized in that, In step six, the atomization pressure of the fluidized bed coater is 0.8 - 1.2 bar, the liquid spraying rate is 6 - 10 mL / min, and the coating thickness is 20 - 30 μm.

10. The preparation method of an emotion-relieving capsule based on a composite formula according to claim 3, characterized in that, In step two, the enzyme inactivation treatment is carried out by heating in a boiling water bath for 10 min. The inlet air temperature for spray drying is 160 - 180 °C, and the outlet air temperature is 75 - 85 °C.

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