HCV quantitative composition, kit, method and application

By introducing homologous vectors and quantitative vectors in HCV detection, the problem of insufficient detection accuracy of low viral load samples is solved, and higher detection stability and accuracy are achieved.

CN119710092APending Publication Date: 2025-03-28SANSURE BIOTECH INC
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Patent Information

Application Number
CN202510079240.3
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-01-17
Publication Date
2025-03-28

AI Technical Summary

Technical Problem

The prior art is difficult to effectively detect HCV with low viral load, resulting in insufficient detection accuracy and stability.

Method used

By introducing homologous vectors, the concentration of HCV in the sample is artificially increased, thereby improving the stability and accuracy of the PCR amplification reaction, and using quantitative vectors to modify the nucleotide sequence and simplifying the quantitative calculation process.

Benefits of technology

The accuracy and stability of HCV detection for low viral load samples is significantly improved, ensuring accurate quantities under low concentration conditions.

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Abstract

The invention belongs to the field of molecular biological detection, particularly relates to a quantitative composition, a kit, a method and application, and more particularly relates to an HCV quantitative composition, a kit, a method and application. The present invention provides a quantitative composition comprising a homologous vector of at least one portion of at least one of at least one of the 5 'UTR region, CORE region, E1 region, E2 region, P7 region, NS2 region, NS3 region, NS4A region, NS4B region, NS5A region, NS5 region, or 3' UTR region of said HCV, and a quantitative vector, as well as primers and probes for amplifying both vectors. The homologous vector is creatively introduced, and the concentration of the sample to be detected is artificially increased, so that the stability and accuracy of PCR amplification reaction are improved. Particularly, in a sample with low virus load, the HCV can be well detected.
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