A kind of sunflower seed extract and its preparation method and application

Abstract

The invention discloses a sunflower extract and its preparation method and application, which relates to the field of sunflower extraction technology, including high-speed homogenization-ultrasonic-assisted liquid phase extraction and macroporous adsorption resin purification and impurity removal methods to obtain the sunflower extract, and also includes the following specific preparation steps: Step 1: crushing treatment, taking a number of sunflower dried flowers, crushing and sieving to obtain a dark yellow powder; Step 2: alcohol solvent-high-speed homogenization-ultrasonic-assisted liquid phase extraction; Step 3: macroporous resin separation and purification; Step 4: obtaining a brown-yellow powder with a slightly bitter taste, i.e., the sunflower extract. The sunflower extract and its preparation method, combined with the application of high-speed homogenization-ultrasonic-assisted liquid phase extraction, have the advantages of fast extraction speed, high extraction yield, sufficient extraction and retention of effective ingredients, simple process, etc. Flavonoid compounds play an important role in human health; it is not only used in health products, but also in cosmetics, medicine, food, agriculture and other fields.

Description

Technical Field

[0001] The invention relates to the technical field of sunflower seedling extraction, and in particular to a sunflower seedling extract and a preparation method and application thereof. Background Art

[0002] Because sunflower contains flavonoids and other effective active ingredients, its extracts have been widely used in cosmetics, food and other fields through extraction technology. The preparation of sunflower extract generally uses water or alcohol solvents as the extracting liquid, and mechanical stirring is used to destroy plant tissues to leach the effective ingredients. Enzymatic hydrolysis can also be used to destroy plant tissue cells through enzymatic hydrolysis.

[0003] Traditional extraction methods often use water as the extraction solvent, which may cause a large amount of leaching of mucilage and water-soluble components such as polysaccharides and tannins in the sunflower, which not only increases the viscosity of the extract and makes it difficult to filter, but also increases the impurity components in the extract and reduces the total flavonoid content. Even if alcohol solvents are used, there are still problems such as long extraction time, low extraction efficiency, and high solvent consumption. Conventional reflux extraction methods increase the leaching of flavonoids and improve the extraction efficiency by increasing the temperature and increasing the contact area between the medicinal material powder and the solvent. However, a high temperature environment can also cause the decomposition and deterioration of some flavonoids, resulting in the loss of effective ingredients and safety considerations under industrial production. Enzyme hydrolysis can destroy plant tissue cells through enzymolysis, promote the dissolution and release of flavonoids, and improve extraction efficiency. However, this will also lead to the degradation of some flavonoids, the loss of effective monomer components, and the enzyme inactivation link of the enzymolysis process may also lead to safety considerations under industrial production. Summary of the invention

[0004] In view of the deficiencies in the prior art, the present invention provides a Hibiscus sibiricus extract and a preparation method and application thereof, which solve the problems raised in the above-mentioned background technology.

[0005] To achieve the above objectives, the present invention is implemented by the following technical scheme: a sunflower extract, comprising a high-speed homogenization-ultrasonic assisted liquid phase extraction, a macroporous adsorption resin purification and impurity removal method, to obtain the sunflower extract;

[0006] The chemical components of sunflower include collagen, dietary fiber, trace elements, vitamin E, linolenic acid, linoleic acid, calcium, zinc, selenium and flavonoids.

[0007] A method for preparing the sunflower seed extract used for the sunflower seed extract comprises the following preparation steps:

[0008] Step 1: Grinding treatment: take some dried golden sunflower flowers, grind them and sieve them (No. 3 sieve, 50 mesh) to obtain dark yellow powder;

[0009] Step 2: alcohol solvent-high-speed homogenization-ultrasonic-assisted liquid phase extraction;

[0010] Step 3: separation and purification by macroporous resin;

[0011] Step 4: obtain a brown-yellow powder with a slightly bitter taste, which is the sunflower seed extract.

[0012] Optionally, the specific process of step 2 is as follows:

[0013] a. Preparation of extract;

[0014] b. High-speed homogenization, homogenization speed 4000rpm~9000rpm, homogenization time 30min~120min;

[0015] c. Ultrasonic assisted extraction;

[0016] d. Filter, remove solids from the extract, and combine the filtrates;

[0017] e. Concentrate and concentrate the filtrate under reduced pressure to recover ethanol as much as possible.

[0018] Optionally, the extract in step a is prepared with an ethanol solution having a concentration of 50% to 80%, and the medicinal material powder and the solution are mixed according to a material-liquid ratio of 1:5 g / ml to 1:100 g / ml.

[0019] Optionally, in step c, the ultrasonic extraction temperature is 30 to 90° C., and the ultrasonic extraction time is 10 to 120 minutes.

[0020] Optionally, the specific process of step three is as follows:

[0021] a. Macroporous resin pretreatment;

[0022] b. Sample preparation: dilute the extract with water to a concentration of 0.2 g of raw material per ml;

[0023] c. Macroporous adsorption resin column purification;

[0024] d. Dry, collect the eluate, recover ethanol under reduced pressure until there is no alcohol smell, continue to concentrate, and dry in a vacuum drying oven until the water content is less than 5%.

[0025] Optionally, in step a, the AB-8 macroporous adsorption resin is soaked in 95% ethanol for 24 hours to fully swell, and then the column bed is rinsed with 95% ethanol, and then rinsed with distilled water until there is no alcohol taste; soaked in 5% hydrochloric acid and NaOH solutions for 2 hours respectively, rinsed with distilled water until neutral, and finally washed with 95% ethanol until there is no white turbidity, and washed with water until there is no alcohol taste for use.

[0026] Optionally, in step c, the volume ratio of the macroporous adsorption resin to the upper column extract is 1:1-1:2, and the adsorption rate is 1-2BV / h; after the adsorption is completed, it is quickly rinsed with 4-8BV of pure water, and the effluent is discarded; followed by a quick rinse with 0.5BV of 5% ethanol solution, and the effluent is discarded; 2-6BV of 40-90% ethanol solution is used to enrich the total flavonoids, and the elution rate is 1-2BV / h, and the eluate is collected.

[0027] An application of the sunflower extract according to any one of the above claims, comprising preparing an oral solution of flavonoid compounds based on the sunflower extract extracted as above;

[0028] The preparation method of the flavonoid compound oral solution is as follows:

[0029] (1) preparing a certain amount of sunflower extract powder and putting it into a mixer, and adding a certain amount of distilled water into the mixer, the mixing mass ratio of the sunflower extract powder to the distilled water being 1-1.5:10, and stirring and mixing evenly until the sunflower extract powder is completely dissolved;

[0030] (2) heating and concentrating the mixture obtained in step (1) to 120-145° C. for 100-120 min, then cooling to 85-90° C. for 4-6 h until the water content reaches 60-75%;

[0031] (3) filtering the concentrated liquid to remove powder particles in the liquid;

[0032] (4) Cooling to room temperature and packaging in equal amounts to obtain a finished flavonoid compound oral liquid.

[0033] The present invention provides a sunflower seed extract and a preparation method and application thereof, which have the following beneficial effects:

[0034] Compared with the traditional solvent extraction method, the preparation method of the sunflower extract adopts dilute alcohol extraction, applies high-speed homogenization-ultrasonic assisted liquid extraction (HSH-UAE), and selects macroporous adsorption resin for separation and purification;

[0035] The use of alcohol solvents is conducive to the extraction of flavonoid components, while preventing the large amount of mucus leaching caused by water as the solvent, and avoiding the problem of difficulty in filtration caused by the increased viscosity of the extract; the high-speed homogenization method increases the contact area between the solvent and the golden sunflower, ensuring that the medicinal materials and the solvent are fully in contact, and the flavonoids are more fully dissolved, thereby accelerating the extraction speed and improving the extraction efficiency; the ultrasonic-assisted extraction method uses mechanical vibration to make the plant tissue cells more completely broken, which can reduce the time and increase the extraction efficiency;

[0036] At the same time, the medicinal materials are extracted under a mild dynamic environment with a low heating temperature, which avoids the destruction of the structure of flavonoids due to thermal and cavitation effects, and effectively reduces the impurity content in the extract.

[0037] The preparation method of the sunflower extract adopts a macroporous adsorption resin separation and impurity removal method, has good purification effect, is easy to operate, safe, non-toxic and pollution-free, and can be reused after a slight treatment after use, effectively reducing production costs. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] Figure 1 This is a schematic diagram of the preparation process of the sunflower seed extract in the invention;

[0039] Figure 2 This is a comparison chart of the total flavonoids content in different parts of the sunflower seed in the invention. DETAILED DESCRIPTION

[0040] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0041] Embodiment 1: Figure 1 As shown, a method for preparing a sunflower seed extract comprises the following preparation steps:

[0042] Step 1: Grinding treatment: take some dried golden sunflower flowers, grind them and sieve them (No. 3 sieve, 50 mesh) to obtain dark yellow powder;

[0043] Step 2: Alcohol solvent-high-speed homogenization-ultrasonic-assisted liquid phase extraction. The specific process is as follows:

[0044] a. Preparation of extract: the extract is prepared with 50% to 80% ethanol solution, and the medicinal material powder and solution are mixed according to the material-liquid ratio of 1:5g / ml to 1:100g / ml;

[0045] b. High-speed homogenization, homogenization speed 4000rpm~9000rpm, homogenization time 30min~120min;

[0046] c. Ultrasonic assisted extraction, ultrasonic extraction temperature 30 ~ 90 ° C, ultrasonic extraction time 10min ~ 120min;

[0047] d. Filter, remove solids from the extract, and combine the filtrates;

[0048] e. Concentrate, concentrate the filtrate under reduced pressure, and recover ethanol as much as possible;

[0049] Step 3: separation and purification by macroporous resin. The specific process is as follows:

[0050] a. Pretreatment of macroporous resin: soak AB-8 macroporous adsorption resin in 95% ethanol for 24 hours to fully swell it, then rinse the column bed with 95% ethanol, and then rinse with distilled water until there is no alcohol smell; soak it in 5% hydrochloric acid and NaOH solution for 2 hours respectively, then rinse it with distilled water until it is neutral, and finally wash it with 95% ethanol until there is no white turbidity, and then wash it with water until there is no alcohol smell for later use;

[0051] b. Sample preparation: dilute the extract with water to a concentration of 0.2 g of raw material per ml;

[0052] c. Purification by macroporous adsorption resin column, the volume ratio of macroporous adsorption resin to upper column extract is 1:1-1:2, and the adsorption rate is 1-2BV / h; after the adsorption, it is quickly rinsed with 4-8BV pure water, and the effluent is discarded; then it is quickly rinsed with 0.5BV 5% ethanol solution, and the effluent is discarded; 2-6BV 40-90% ethanol solution is used to enrich the total flavonoids, and the elution rate is 1-2BV / h, and the eluate is collected;

[0053] d. Drying, collecting the eluate, recovering ethanol under reduced pressure until there is no alcohol smell, continuing to concentrate, and drying in a vacuum drying oven until the water content is less than 5%;

[0054] Step 4: obtain a brown-yellow powder with a slightly bitter taste, which is the sunflower seed extract.

[0055] Embodiment 2: Figure 1 As shown, a method for preparing a sunflower seed extract comprises the following steps:

[0056] S1: Weigh and crush dried Helianthus annuus flowers, pass through a 50-mesh No. 3 sieve, and add them into a 70% ethanol solution; the mass volume ratio of Helianthus annuus to water is 1 g: 5 mL;

[0057] S2: high-speed homogenization at 4000 rpm for 15 min, ultrasonic extraction at 30 °C for 30 min to obtain a crude extract;

[0058] S3: Filter the crude extract to obtain filtrate A, recover ethanol under reduced pressure until it is exhausted, and dilute with water;

[0059] S4: pre-treating the AB-8 macroporous resin by soaking it in 95% ethanol by volume for 24 hours, soaking it in 5% hydrochloric acid and sodium hydroxide for 2 hours respectively, and washing it with pure water until it is neutral; using the AB-8 macroporous resin column for adsorption extraction, washing impurities with pure water, and then eluting with ethanol to obtain an eluate;

[0060] S5: The eluate is concentrated and dried to obtain a sunflower seed extract, which is crushed to obtain a brown-yellow powder with a bitter taste.

[0061] Example 3: An application of a sunflower extract, comprising preparing a flavonoid compound oral solution based on the sunflower extract extracted as described above;

[0062] The preparation method of the flavonoid compound oral solution is as follows:

[0063] (1) preparing a certain amount of sunflower extract powder and putting it into a mixer, and adding a certain amount of distilled water into the mixer, the mixing mass ratio of the sunflower extract powder to the distilled water being 1-1.5:10, and stirring and mixing evenly until the sunflower extract powder is completely dissolved;

[0064] (2) heating and concentrating the mixture obtained in step (1) to 120-145° C. for 100-120 min, then cooling to 85-90° C. for 4-6 h until the water content reaches 60-75%;

[0065] (3) filtering the concentrated liquid to remove powder particles in the liquid;

[0066] (4) Cooling to room temperature and packaging in equal amounts to obtain a finished flavonoid compound oral liquid.

[0067] Flavonoids have many functions such as anti-cardiovascular and cerebrovascular diseases, anti-oxidation, anti-aging, analgesia, anti-inflammatory, anti-tumor, and antiviral, and play an important role in human health. Among them, flavonoids can not only be used in health products, but also in cosmetics, medicine, food, agriculture, etc.

[0068] Health products: As a natural health ingredient, it can be used in the field of health products, such as dietary supplements, nutritional health products, functional foods, etc.

[0069] Cosmetics; flavonoids have antioxidant, anti-inflammatory and whitening effects, and are used in the cosmetics field such as skin care products and beauty products;

[0070] Medical field: Flavonoids have anti-cancer, lipid-lowering, and blood sugar-lowering effects, and can be used to manufacture anti-cancer drugs, lipid-lowering drugs, and blood sugar-lowering drugs;

[0071] Food industry: such as the production of beverages, juices, candies, chocolates, etc.;

[0072] Agricultural field: Flavonoids have plant growth regulating effects and are widely used in the agricultural field, such as promoting plant growth and increasing crop yields.

[0073] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A sunflower extract, characterized in that The method includes high-speed homogenization-ultrasonic-assisted liquid phase extraction and macroporous adsorption resin purification and impurity removal to obtain the sunflower seed extract; The chemical components of sunflower include collagen, dietary fiber, trace elements, vitamin E, linolenic acid, linoleic acid, calcium, zinc, selenium and flavonoids.

2. A method for preparing the sunflower seed extract according to claim 1, characterized in that: The method comprises the following preparation steps: Step 1: Grinding treatment: take some dried flowers of golden sunflower, grind them and sieve them to obtain dark yellow powder; Step 2: alcohol solvent-high-speed homogenization-ultrasonic-assisted liquid phase extraction; Step 3: separation and purification by macroporous resin; Step 4: obtain a brown-yellow powder with a slightly bitter taste, which is the sunflower seed extract.

3. The method for preparing a Hibiscus sibiricum extract according to claim 2, characterized in that: The specific process of step 2 is as follows: a. Preparation of extract; b. High-speed homogenization, homogenization speed 4000rpm~9000rpm, homogenization time 30min~120min; c. Ultrasonic assisted extraction; d. Filter, remove solids from the extract, and combine the filtrates; e. Concentrate and concentrate the filtrate under reduced pressure to recover ethanol as much as possible.

4. The method for preparing a Hibiscus sibiricum extract according to claim 3, characterized in that: The extract in step a is prepared into an ethanol solution with a concentration of 50% to 80%, and the medicinal material powder and the solution are mixed according to a material-liquid ratio of 1:5 g / ml to 1:100 g / ml.

5. The method for preparing the Hibiscus sibiricus extract according to claim 3, characterized in that: In the step c, the ultrasonic extraction temperature is 30 to 90° C., and the ultrasonic extraction time is 10 to 120 minutes.

6. The method for preparing the Hibiscus sibiricus extract according to claim 2, characterized in that: The specific process of step three is as follows: a. Macroporous resin pretreatment; b. Sample preparation: dilute the extract with water to a concentration of 0.2 g of raw material per ml; c. Macroporous adsorption resin column purification; d. Dry, collect the eluate, recover ethanol under reduced pressure until there is no alcohol smell, continue to concentrate, and dry in a vacuum drying oven until the water content is less than 5%.

7. The method for preparing the Ambrette extract according to claim 6, characterized in that: In the step a, AB-8 macroporous adsorption resin is soaked in 95% ethanol for 24 hours to fully swell, and then the column bed is rinsed with 95% ethanol, and then rinsed with distilled water until there is no alcohol taste; after soaking in 5% hydrochloric acid and NaOH solution for 2 hours respectively, it is rinsed with distilled water until it is neutral, and finally washed with 95% ethanol until there is no white turbidity, and washed with water until there is no alcohol taste for use.

8. The method for preparing the Hibiscus sibiricus extract according to claim 6, characterized in that: In the step c, the volume ratio of the macroporous adsorption resin to the upper column extract is 1:1-1:2, and the adsorption rate is 1-2BV / h; after the adsorption is completed, it is quickly rinsed with 4-8BV of pure water, and the effluent is discarded; it is then quickly rinsed with 0.5BV of 5% ethanol solution, and the effluent is discarded; 2-6BV of 40-90% ethanol solution is used to enrich the total flavonoids, and the elution rate is 1-2BV / h, and the eluate is collected.

9. A use of the sunflower extract according to any one of claims 1 to 8, characterized in that: The method comprises preparing a flavonoid compound oral liquid based on the sunflower seed extract extracted as above; The preparation method of the flavonoid compound oral solution is as follows: (1) preparing a certain amount of sunflower extract powder and putting it into a mixer, and adding a certain amount of distilled water into the mixer, the mixing mass ratio of the sunflower extract powder to the distilled water being 1-1.5:10, and stirring and mixing evenly until the sunflower extract powder is completely dissolved; (2) heating and concentrating the mixture obtained in step (1) to 120-145° C. for 100-120 min, then cooling to 85-90° C. for 4-6 h until the water content reaches 60-75%; (3) filtering the concentrated liquid to remove powder particles in the liquid; (4) Cooling to room temperature and packaging in equal amounts to obtain a finished flavonoid compound oral liquid.

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