Beta-ketoester acyl-CoA reductase KCR gene and application thereof in improving salt tolerance of plants
By cloning and overexpressing the KCR gene of grape β-ketoestyl-CoA reductase, the synthesis of epidermal waxes and ultra-long chain fatty acids was regulated, and the problem of growth restriction in grapes under saline and alkali stress was solved, and the salt tolerance of grapes was significantly improved.
CN119776387AActive Publication Date: 2025-04-08QINGDAO AGRI UNIV
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Patent Information
- Application Number
- CN202510052507.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-14
- Publication Date
- 2025-04-08
- Estimated Expiration
- 2045-01-14
AI Technical Summary
Technical Problem
Grapes are limited in growth under saline-alkali stress, resulting in a decrease in yield and quality. The prior art is difficult to effectively improve the adaptability of grapes to salinized soil.
Method used
By cloning and overexpressing the KCR gene of β-ketoyl-CoA reductase in grapes, the accumulation of epidermal waxes and ultra-long chain fatty acid biosynthesis are regulated, thereby improving the salt tolerance of plants.
Benefits of technology
Overexpression of the KCR gene allows transgenic grape callus to show higher salt tolerance under salt stress, significantly improving the accumulation of epidermal waxes and the biosynthesis of ultra-long chain fatty acids, and enhancing the resistance of plants to salt stress.
✦ Generated by Eureka AI based on patent content.
Abstract
The invention relates to the technical field of plant genetic engineering. The invention provides a beta-ketoester acyl-CoA reductase KCR gene, wherein the nucleotide sequence of the KCR gene is as shown in SEQ ID NO. 1. The invention also provides a recombinant vector. The invention also provides a recombinant bacterium. The invention also provides an application of the KCR gene, the recombinant vector or the recombinant bacterium in improving the salt tolerance of plants. The invention also provides application of the KCR gene, the recombinant vector or the recombinant bacterium in plant salt tolerance genetic improvement. The invention further provides a method for obtaining the salt-tolerant plant. After the KCR gene is over-expressed in the grape callus, the salt tolerance of the obtained transgenic grape callus is obviously improved. And after the gene is over-expressed in arabidopsis thaliana, the obtained transgenic arabidopsis thaliana epidermis wax is accumulated and the salt tolerance is obviously increased, so that a new gene resource can be provided for salt tolerance improvement and germplasm resource breeding of a grape cultivation variety.
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