Plasma freezing process parameter detection method and device

Abstract

The invention relates to the technical field of plasma freezing process parameter detection, in particular to a plasma freezing process parameter detection method and device.The plasma freezing process parameter detection device comprises an equipment base, a simulated plasma barrel is fixedly arranged on the equipment base, a shaking base is rotationally connected to the equipment base, and a flushing pipe is rotationally connected to the shaking base; the outer wall of one side of the flushing pipe is fixedly connected with the scrubbing mechanism, the simulated plasma bag is placed on the equipment base, the parameter collecting mechanism is installed on the outer side of the simulated plasma bag, and the control panel is installed on the equipment base. The plasma freezing parameter and the plasma instant freezer parameter are calibrated under the condition of simulating the conventional state of plasma freezing, the device has a low-temperature counting function, it is ensured that the plasma simulation body can simulate the plasma physical property, the use is convenient, the result is reliable, calculation is automatic, meanwhile, the accuracy and reliability of the plasma freezing process parameter can be comprehensively evaluated, and the operation is convenient. And a data support is provided for ensuring the plasma cryopreservation quality and carrying out related research on plasma.

Description

Technical Field

[0001] The present invention relates to the technical field of plasma freezing process parameter detection, and in particular to a plasma freezing process parameter detection method and device. Background Art

[0002] The main function of the plasma quick freezer is to quickly freeze and preserve blood products such as plasma, platelets and white blood cells in a precisely controlled low-temperature environment. This process is crucial. Through efficient freezing technology, various coagulation factors and other bioactive substances in plasma can maintain their activity to the greatest extent, and can effectively inhibit the reproduction of pathogenic microorganisms, thereby ensuring the quality and safety of blood products and providing strong support for subsequent clinical treatment. As a precious medical resource, the entire process of plasma collection, storage and use must be efficient and reasonable. Accurate temperature parameter calibration can help medical institutions more accurately grasp the actual operating conditions of the plasma quick freezer, reasonably arrange maintenance plans, extend the service life of the equipment, reduce plasma waste caused by equipment problems, effectively reduce plasma deterioration or invalidity caused by inaccurate equipment temperature system, reduce medical risks and avoid medical disputes caused by this. In addition, ensuring the safe operation of equipment, scientifically preserving laboratory biological products, and reducing clinical medical risks are also the focus of scientific researchers, doctors and patients. The accuracy and stability of its temperature parameters, freezing rate, etc. directly affect the quality and safety of blood products. Scientific and effective metrological traceability of laboratory biological product low-temperature storage equipment is an effective way to solve the above problems.

[0003] Chinese patent document CN202322666676.1 provides "A temperature calibration device for a plasma quick freezer", which includes a temperature patrol meter and multiple plasma simulation bags. Through the cooperation of the sleeve rod and the hollow round rod thread, the temperature sensor is moved to the inside of the plasma simulation bag, and the temperature sensor is kept in a relatively fixed state, so that it can be controlled by the temperature patrol meter to detect the temperature of the physiological saline in the liquid storage chamber, detect multiple groups of temperatures, and calculate the temperature deviation, uniformity, fluctuation and cooling rate of the plasma freezer.

[0004] However, this technical solution still has shortcomings: 1. The calibration method of the inspection instrument cannot put the main unit into the plasma quick freezer, and the calibration cannot be guaranteed in the sealed working state of the plasma quick freezer, which will destroy the sealing of the plasma quick freezer, and the obtained temperature test data will be affected by the environmental heat exchange; 2. Due to the complexity of plasma itself, the solid-liquid phase transition occurs in a certain temperature range. After reaching the initial freezing temperature, the ice content changes continuously. What really affects the quality of plasma freezing is the plasma freezing process parameters. This technical solution cannot be used for plasma freezing parameters, such as pre-cooling stage time, cooling rate, crystallization stage temperature, crystallization time, deep cooling stage time, cooling rate, supercooling state minimum temperature, time, etc. 3. During the freezing process of the plasma bag, the freezing state of different areas is different. This patent only detects the center point of the plasma bag, and lacks detection of the distribution of different areas during the freezing process of the plasma bag. 4. Physiological saline is used to simulate plasma, and there are differences in characteristics, which cannot simulate the physical and chemical changes of the plasma freezing process.

[0005] Based on this, the present invention designs a method and device for detecting parameters of plasma freezing process to solve the above problems. Summary of the invention

[0006] The purpose of the present invention is to solve the shortcomings of the background technology and to propose a method and device for detecting parameters of plasma freezing process.

[0007] To achieve the above objectives, the technical solution adopted by the present invention is: a plasma freezing process parameter detection device, comprising an equipment base, a simulated plasma barrel is fixedly arranged on the equipment base, a shaking base is rotatably connected to the equipment base, a flushing tube is rotatably connected to the shaking base, a brushing mechanism is fixedly connected to the outer wall of one side of the flushing tube, a simulated plasma bag is placed on the equipment base, a parameter collection mechanism is installed on the outside of the simulated plasma bag, and a control panel is installed on the equipment base.

[0008] Preferably, a liquid injection port is fixedly provided at the top end of the simulated plasma bag, a liquid discharge port is fixedly provided at one side of the bottom end of the simulated plasma bag, and sealing covers are threadedly provided on the outer sides of the liquid injection port and the liquid discharge port.

[0009] Preferably, the parameter acquisition mechanism includes a shell, which is fixedly connected to the outer wall of the simulated plasma bag, a circuit board is installed in the shell, a plurality of wires are fixedly connected to the circuit board, the wires extend to the interior of the simulated plasma bag and are fixedly connected to a temperature sensor, and a low-temperature battery is installed in the shell.

[0010] Preferably, a pump body is fixedly provided on the simulated plasma barrel through a pipeline connection, the pump body is fixedly connected to the upper surface of the equipment seat, a connecting hose is fixedly provided on the output end of the pump body, the other end of the connecting hose passes through the equipment seat and is rotatably connected to a connecting cover A, the connecting cover A is threadedly connected to the injection port, and the connecting cover A is threadedly connected to a sealing plug fixedly connected to the equipment seat.

[0011] Preferably, a drain pipe is connected to one side of the shaking seat, and a connection cover B is rotatably connected to the outer wall of one end of the drain pipe, and the connection cover B is threadedly connected to the outer wall of the drain port.

[0012] Preferably, a motor is provided on one side of the rocking seat, the motor is connected and fixedly arranged with the equipment seat, an L-shaped rod is connected and fixedly arranged at the output end of the motor, and the other end of the L-shaped rod is slidably matched with the inner wall of the slide groove opened on the rocking seat.

[0013] Preferably, a water-conducting hose is rotatably connected to the top of the flushing pipe, a transmission wheel is fixedly connected to the outer wall of the upper end of the flushing pipe, an arc-shaped rack is meshingly provided on one side of the transmission wheel, the arc-shaped rack is fixedly connected to the equipment seat, a connecting cover C is rotatably connected to the outer wall of the flushing pipe, the connecting cover C is threadedly connected to the liquid injection port, and a plurality of nozzles are fixedly connected to the flushing pipe.

[0014] Preferably, the brushing mechanism includes a fixed frame, which is connected and fixed to the outer wall of the flushing pipe, and two connecting rod groups are symmetrically structured and rotatably connected inside the fixed frame, and a brushing rod is rotatably connected between the other ends of the two connecting rod groups, and a spring is connected and fixed to the brushing rod, and the other end of the spring is connected and fixed to the inner wall of the fixed frame, and an electric push rod is connected and fixed to the inner wall of the fixed frame, and an adjusting rack is connected and fixed to the output end of the electric push rod, and one end of one of the connecting rod groups connected to the fixed frame is connected and fixed to an adjusting wheel, and the adjusting wheel is meshed with the adjusting rack for transmission.

[0015] A method for detecting parameters of plasma freezing process comprises the following steps:

[0016] S1. First, inject simulated plasma into the simulated plasma barrel, and then inject an appropriate amount of simulated plasma into the simulated plasma bag through the control panel;

[0017] S2, then placing the simulated plasma bag inside the plasma quick-freezing machine, and collecting temperature data through the parameter collection mechanism;

[0018] S3. After freezing for 4 hours, take out the samples and read the freezing process curve of the parameter collection mechanism through Bluetooth communication. The software will automatically analyze the freezing process curve and calculate the temperature parameters of the freezing process, including precooling stage time, cooling rate, crystallization stage temperature, crystallization time, deep freezing stage time, cooling rate, minimum temperature and time of supercooling state, temperature deviation of plasma freezer, and temperature uniformity;

[0019] S5. Then pour out the simulated plasma in the simulated plasma bag after it melts, and then install the simulated plasma bag on the rocking seat, insert the flushing tube into the simulated plasma bag, and when the rocking seat swings, it will drive the flushing tube and the brushing mechanism to rotate, so as to flush and brush the inner wall of the simulated plasma bag.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] 1. Through the adoption of wireless design, non-interference can be achieved. The plasma freezing parameters and plasma quick-freezing machine parameters can be calibrated under the normal conditions of simulated plasma freezing. The device has a low-temperature counting function to ensure that the plasma simulation body can simulate the physical properties of plasma. In terms of use, the results are reliable and automatically calculated, which reduces the error of manual calibration. It can be portable and reliably used for the calibration of plasma quick-freezing machines. At the same time, it can comprehensively evaluate the accuracy and reliability of plasma freezing process parameters, providing data support for ensuring the quality of plasma freezing and conducting plasma-related research;

[0022] 2. By setting up a simulated plasma barrel, the pump can be controlled through the control panel to replace manual work and automatically inject an accurate amount of simulated plasma into the simulated plasma bag, which can greatly improve the experimental efficiency and reduce the preparation time for each experiment, thereby ensuring the quality of the experiment and the reliability of the data;

[0023] 3. By setting up a shaking seat, a flushing tube and a brushing mechanism, the simulated plasma bag can be automatically flushed and cleaned after use, avoiding the problem of missing certain areas or incomplete cleaning during manual cleaning, which will affect the accuracy of the next experiment. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 It is a schematic diagram of the overall structure of a method and device for detecting parameters of plasma freezing process of the present invention;

[0025] Figure 2 It is a schematic diagram of the overall structure of a plasma freezing process parameter detection method and device of the present invention during cleaning;

[0026] Figure 3 It is a cross-sectional expansion schematic diagram of a simulated plasma bag structure of a plasma freezing process parameter detection method and device of the present invention;

[0027] Figure 4It is a partial cross-sectional schematic diagram of the parameter acquisition mechanism structure of a plasma freezing process parameter detection method and device of the present invention;

[0028] Figure 5 It is a schematic diagram of the structure of a simulated plasma barrel, etc. of a method and device for detecting parameters of plasma freezing process of the present invention;

[0029] Figure 6 It is a partial cross-sectional schematic diagram of a rocking seat structure of a method and device for detecting parameters of plasma freezing process of the present invention;

[0030] Figure 7 It is a partial cross-sectional schematic diagram of a flushing pipe structure of a plasma freezing process parameter detection method and device of the present invention;

[0031] Figure 8 The present invention is a partial cross-sectional schematic diagram of the structure of a brushing mechanism of a method and device for detecting parameters in a plasma freezing process.

[0032] The following are marked in the figure: 1. Equipment seat; 2. Simulated plasma barrel; 3. Shaking seat; 4. Flushing tube; 5. Scrubbing mechanism; 6. Simulated plasma bag; 7. Parameter collection mechanism; 8. Control panel; 601. Liquid injection port; 602. Liquid discharge port; 603. Sealing cover; 701. Housing; 702. Circuit board; 703. Wire; 704. Temperature sensor; 201. Pump body; 202. Connection cover A; 203. Connection hose; 101, sealing plug; 301, drain pipe; 302, connecting cover B; 303, motor; 304, L-shaped rod; 305, slide groove; 401, water guide hose; 402, transmission wheel; 403, arc-shaped rack; 404, connecting cover C; 405, nozzle; 501, fixing bracket; 502, connecting rod group; 503, brush rod; 504, spring; 505, electric push rod; 506, adjustment rack; 507, adjustment wheel. DETAILED DESCRIPTION

[0033] The following description is used to disclose the present invention so that those skilled in the art can implement the present invention. The preferred embodiments described below are only examples, and those skilled in the art may think of other obvious variations.

[0034] like Figure 1-Figure 8 The method and device for detecting parameters of a plasma freezing process shown in the figure include an equipment base 1, on which a simulated plasma barrel 2 is fixedly arranged, a shaking base 3 is rotatably connected to the equipment base 1, a flushing tube 4 is rotatably connected to the shaking base 3, a brushing mechanism 5 is fixedly arranged on the outer wall of one side of the flushing tube 4, a simulated plasma bag 6 is placed on the equipment base 1, a parameter collection mechanism 7 is installed on the outer side of the simulated plasma bag 6, and a control panel 8 is installed on the equipment base 1.

[0035] like Figure 3As shown, a liquid injection port 601 is fixedly provided at the top of the simulated plasma bag 6, a liquid discharge port 602 is fixedly provided at one side of the bottom of the simulated plasma bag 6, and sealing covers 603 are threadedly provided on the outer sides of the liquid injection port 601 and the liquid discharge port 602.

[0036] like Figure 4 As shown, parameter acquisition mechanism 7 comprises shell 701, and shell 701 is connected and fixedly arranged with the outer wall of simulation plasma bag 6, and circuit board 702 is installed in shell 701, and multiple wires 703 are connected and fixedly arranged on circuit board 702, and wire 703 extends to the inside of simulation plasma bag 6 and is connected and fixedly arranged with temperature sensor 704, and low temperature battery is installed in shell 701. Temperature data acquisition, storage and transmission circuit are distributed on circuit board 702, including single chip microcomputer, AD, memory, crystal oscillator and Bluetooth antenna element. Connection can be established with computer software through Bluetooth, and sampling interval and sampling time of parameter acquisition mechanism 7 can be controlled by software, and temperature data collected are read and analyzed. Wireless device is arranged outside plasma simulation body, and temperature sensor 704 probe is distributed and arranged at 5 positions inside plasma simulation body, namely upper left, upper right, geometric center, lower left and lower right. The parameter acquisition mechanism 7 is powered by a low-temperature battery. In order to minimize the impact of the wireless temperature measuring device on the plasma quick-freezing device and support the detection of more specifications of equipment, the control and wireless modules exposed to the outside are integrated on a small circuit board 702 and encapsulated in a housing 701. The parameter acquisition mechanism 7 has very low standby power consumption and low power consumption when working, and the temperature sampling time and sampling period can be set. In addition, it has a certain amount of data storage and is easy to read. In actual use, the device can perform stable temperature detection in a low temperature environment of -70°C.

[0037] like Figure 5 As shown, a pump body 201 is fixedly provided on the simulated plasma barrel 2 through a pipeline connection, the pump body 201 is fixedly provided on the upper surface of the equipment seat 1, a connecting hose 203 is fixedly provided on the output end of the pump body 201, the other end of the connecting hose 203 passes through the equipment seat 1 and is rotatably connected to a connecting cover A202, the connecting cover A202 is threadedly provided with the injection port 601, and the connecting cover A202 is threadedly provided with a sealing plug 101 fixedly provided on the equipment seat 1. The sealing plug 101 realizes sealing protection of the connecting cover A202 when it is not in use.

[0038] like Figure 6 As shown, a drain pipe 301 is connected to one side of the shaking seat 3, and a connection cover B302 is rotatably connected to the outer wall of one end of the drain pipe 301, and the connection cover B302 is threadedly connected to the outer wall of the drain port 602;

[0039] A motor 303 is arranged on one side of the shaking seat 3, and the motor 303 is connected and fixedly arranged with the equipment seat 1, and an L-shaped rod 304 is connected and fixedly arranged at the output end of the motor 303, and the other end of the L-shaped rod 304 is slidably matched with the inner wall of the sliding groove 305 opened on the shaking seat 3.

[0040] like Figure 7 As shown, a water guide hose 401 is rotatably connected to the top of the flushing pipe 4, a transmission wheel 402 is fixedly connected to the outer wall of the upper end of the flushing pipe 4, an arc-shaped rack 403 is meshingly transmitted on one side of the transmission wheel 402, the arc-shaped rack 403 is fixedly connected to the equipment seat 1, a connecting cover C404 is rotatably connected to the outer wall of the flushing pipe 4, the connecting cover C404 is threadedly connected to the liquid injection port 601, and a plurality of nozzles 405 are fixedly connected to the flushing pipe 4.

[0041] like Figure 8 As shown, the scrubbing mechanism 5 includes a fixed frame 501, which is connected and fixedly arranged with the outer wall of the flushing tube 4, and two connecting rod groups 502 are rotatably connected and arranged in the fixed frame 501 in a symmetrical structure, and a scrubbing rod 503 is rotatably connected and arranged between the other ends of the two connecting rod groups 502, and a spring 504 is connected and fixedly arranged on the scrubbing rod 503, and the other end of the spring 504 is connected and fixedly arranged with the inner wall of the fixed frame 501, and an electric push rod 505 is connected and fixedly arranged with the inner wall of the fixed frame 501, and an adjusting rack 506 is connected and fixedly arranged at the output end of the electric push rod 505, and an adjusting wheel 507 is connected and fixedly arranged at one end of one connecting rod group 502 connected to the fixed frame 501, and the adjusting wheel 507 is meshed and driven with the adjusting rack 506. The spring 504 enables the scrubbing rod 503 to be attached to the inner wall of the simulated plasma bag 6.

[0042] A method for detecting parameters of plasma freezing process comprises the following steps:

[0043] S1. First, simulated plasma is prepared: simulated plasma composition: 0.1% weight percentage of polyvinyl alcohol, a mass ratio of sodium alginate to polyvinyl alcohol of 2:1, 5% weight percentage of glycerol, and the rest is pure water. The polyvinyl alcohol is dissolved in hot water in a 90°C constant temperature water bath to prepare a 10wt% polyvinyl alcohol solution → weigh sodium alginate powder with a mass ratio of 2:1 to polyvinyl alcohol, dissolve it in pure water in a 85°C constant temperature water bath to prepare a 3.5wt% sodium alginate solution → mix the two and freeze at -18°C for 4h , thaw at room temperature for 2 hours, repeat 3 times to obtain a composite aqueous solution after physical cross-linking; → add 5% by weight of glycerol, and make up the rest with pure water, shake in a metal bath at 2000rpm, 4℃ for 15 minutes to mix evenly, and obtain a simulated plasma solution → measure the freezing point, viscosity, density and specific heat capacity of the simulated plasma, which are all within the range of ±0.5% of the physical and chemical parameters of the plasma, and then inject the simulated plasma into the simulated plasma barrel 2, and then inject an appropriate amount of simulated plasma into the simulated plasma bag 6 through the control panel 8;

[0044] S2, then placing the simulated plasma bag 6 into the plasma quick-freezing machine, and collecting temperature data through the parameter collection mechanism 7;

[0045] S3, after freezing for 4 hours, take out and read the freezing process curve of the parameter acquisition mechanism 7 through Bluetooth communication. The software will automatically analyze the freezing process curve and calculate the temperature parameters of the freezing process, including the pre-cooling stage time, cooling rate, crystallization stage temperature, crystallization time, deep cooling stage time, cooling rate, supercooling state minimum temperature, time, plasma freezer temperature deviation, temperature uniformity,

[0046] Precooling stage time: The software automatically intercepts and calculates the time it takes to drop to the crystallization temperature after the device is placed in:

[0047] ΔT d1 =T c -T1

[0048] Where: T c ——Parameter acquisition mechanism 7 simulates the moment when the plasma temperature drops to the crystallization temperature; T1——the moment when the parameter acquisition mechanism 7 is placed in the plasma quick-freezing machine at the starting temperature.

[0049] Cooling rate: When the device is placed in the crystallization temperature, the cooling rate from the device cooling by 30% to the crystallization temperature is:

[0050]

[0051] Where: t d1 ——cooling rate in the precooling stage, °C / min; t1——the starting temperature when the parameter acquisition mechanism 7 is placed in the plasma quick freezer, °C; t c——plasma crystallization temperature; T c ——Parameter collection mechanism 7 simulates the moment when the plasma temperature drops to the crystallization temperature; T 30 %——the time when the parameter acquisition mechanism 7 is cooled to 30% of the pre-cooling.

[0052] Crystallization stage temperature: the average temperature measured during the stable crystallization stage.

[0053] Crystallization time: the time to maintain the stable crystallization stage.

[0054] Minimum temperature of supercooled state: The temperature in supercooled state drops to the lowest point and then rises to the inflection point temperature of stable crystallization state at the next moment.

[0055] Supercooling state maintenance time: from the time when the temperature of the parameter acquisition mechanism 7 is lowered to the corresponding moment of the crystallization temperature, the temperature continues to be lowered until the temperature is raised to the stable crystallization state at the next moment.

[0056] Deep cooling stage time, cooling rate

[0057]

[0058] Where: t d2 ——cooling rate in deep cooling stage, °C / min; t2——cryogenic temperature when parameter acquisition mechanism 7 enters deep cooling stable stage, °C; t c ——plasma crystallization temperature; T c ——Parameter collection mechanism 7 simulates the moment when the plasma temperature drops to the crystallization temperature; T 50％ ——The time corresponding to when the parameter acquisition mechanism 7 is cooled to 50% of deep cooling.

[0059] Temperature deviation of plasma freezer: the difference between the measured average value of the deep-freezing temperature when the parameter acquisition mechanism 7 enters the deep-freezing stable stage and the set temperature of the plasma freezer.

[0060] Temperature uniformity of plasma freezer: simulate the maximum temperature difference of 5 temperature points in plasma bag 6 at the same time in the deep freezing stabilization stage.

[0061] The parameter acquisition mechanism 7 has a built-in low-temperature working counting function. When the temperature is detected to be less than -25°C and lasts for more than 30 minutes, the internal count of the device is increased by 1, and the cumulative number of low-temperature tests can be seen on the software side. Since the outer packaging bag of the plasma simulator is made of PE material, there is a risk of bag breakage during repeated freezing and thawing. When it is ≤5 times, the simulated plasma bag 6 can work stably and reliably. In addition, the liquid in the plasma simulator has been frozen and thawed many times, and the simulated thermal physical parameters will deviate. In order to avoid excessive deviation from the plasma, when the cumulative number of low temperatures is greater than 5 times, the software side prompts to replace the plasma simulator. When the plasma simulator is replaced and OK is clicked, the count returns to 0. The main unit and the probe part of the temperature sensor 704 can be reused repeatedly. The device can also be expanded for use in the calibration of blood plasma thawing machines to detect the plasma rethawing process;

[0062] S4. Then pour out the simulated plasma in the simulated plasma bag 6 after it melts, and then install the simulated plasma bag 6 on the shaking seat 3, insert the flushing tube 4 into the simulated plasma bag 6, and when the shaking seat 3 swings, it will drive the flushing tube 4 and the brushing mechanism 5 to rotate, so as to flush and brush the inner wall of the simulated plasma bag 6.

[0063] Working principle: When it is necessary to detect the parameters of the plasma freezing process, first open the sealing cover 603 on the discharge port 602 of the simulated plasma bag 6, then remove the connecting cover A202 from the sealing plug 101, and threadedly connect it to the discharge port 602, and then control the pump body 201 through the control panel 8 to draw out the simulated plasma in the simulated plasma barrel 2 and inject it into the simulated plasma bag 6 through the connecting hose 203;

[0064] Then, the sealing cover 603 is covered on the drain port 602, and then the simulated plasma bag 6 is placed in the plasma quick freezer to detect the freezing process parameters. After the detection is completed, the simulated plasma in the simulated plasma bag 6 is melted and then poured out;

[0065] The discharge port 602 of the simulated plasma bag 6 is threadedly connected to the connection cover B302, and then the injection port 601 of the simulated plasma bag 6 is aligned with the flushing tube 4 and threadedly connected to the connection cover C404, and then the electric push rod 505 is used to drive the adjustment rack 506 to disengage from the meshing with the adjustment wheel 507, and under the action of the spring 504, the brush rod 503 connected to the connecting rod group 502 is driven to move out;

[0066] Then, high-pressure cleaning liquid is injected into the flushing pipe 4 through the water guide hose 401, and then the motor 303 is used to drive the L-shaped rod 304 to rotate, so that the other end of the L-shaped rod 304 slides in the slide groove 305, thereby driving the rocking seat 3 to swing back and forth;

[0067] At the same time, under the action of the arc-shaped rack 403, the flushing tube 4 connected to the transmission wheel 402 will rotate, and at the same time drive the scrubbing rod 503 to flush and scrub the inner wall of the simulated plasma bag 6, and the scrubbing waste water will be discharged through the drain pipe 301.

[0068] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The above embodiments and the specification only describe the principles of the present invention. The present invention may be subject to various changes and improvements without departing from the spirit and scope of the present invention. These changes and improvements fall within the scope of the present invention to be protected. The scope of protection claimed by the present invention is defined by the attached claims and their equivalents.

Claims

1. A plasma freezing process parameter detection device, comprising a device base (1), characterized in that: A simulated plasma barrel (2) is fixedly arranged on the equipment base (1), a shaking base (3) is rotatably connected to the equipment base (1), a flushing tube (4) is rotatably connected to the shaking base (3), a brushing mechanism (5) is fixedly arranged on the outer wall of one side of the flushing tube (4), a simulated plasma bag (6) is placed on the equipment base (1), a parameter collection mechanism (7) is installed on the outer side of the simulated plasma bag (6), and a control panel (8) is installed on the equipment base (1).

2. A plasma freezing process parameter detection device according to claim 1, characterized in that: The top of the simulated plasma bag (6) is fixedly connected with an injection port (601), and one side of the bottom of the simulated plasma bag (6) is fixedly connected with a discharge port (602). The outer sides of the injection port (601) and the discharge port (602) are both threadedly connected with sealing covers (603).

3. A plasma freezing process parameter detection device according to claim 1, characterized in that: The parameter acquisition mechanism (7) comprises a shell (701), the shell (701) is connected and fixedly arranged with the outer wall of the simulated plasma bag (6), a circuit board (702) is installed in the shell (701), a plurality of wires (703) are connected and fixedly arranged on the circuit board (702), the wires (703) extend into the interior of the simulated plasma bag (6) and are connected and fixedly arranged with a temperature sensor (704), and a low-temperature battery is installed in the shell (701).

4. A plasma freezing process parameter detection device according to claim 1, characterized in that: A pump body (201) is fixedly provided on the simulated plasma barrel (2) via a pipeline connection, and the pump body (201) is fixedly provided on the upper surface of the equipment base (1). A connecting hose (203) is fixedly provided on the output end of the pump body (201), and the other end of the connecting hose (203) passes through the equipment base (1) and is rotatably connected to a connecting cover A (202), and the connecting cover A (202) is threadedly provided on the injection port (601), and the connecting cover A (202) is threadedly provided on the sealing plug (101) fixedly provided on the equipment base (1).

5. A plasma freezing process parameter detection device according to claim 1, characterized in that: One side of the shaking seat (3) is connected to a drainage pipe (301), and one end of the drainage pipe (301) is rotatably connected to the outer wall thereof with a connection cover B (302), and the connection cover B (302) is threadedly connected to the outer wall of the drainage port (602).

6. A plasma freezing process parameter detection device according to claim 1, characterized in that: A motor (303) is arranged on one side of the shaking seat (3), and the motor (303) is connected and fixedly arranged with the equipment seat (1); an L-shaped rod (304) is connected and fixedly arranged at the output end of the motor (303), and the other end of the L-shaped rod (304) is slidably matched with the inner wall of a sliding groove (305) provided on the shaking seat (3).

7. A plasma freezing process parameter detection device according to claim 1, characterized in that: A water guide hose (401) is rotatably connected to the top of the flushing pipe (4), a transmission wheel (402) is fixedly connected to the outer wall of the upper end of the flushing pipe (4), an arc-shaped rack (403) is meshingly driven on one side of the transmission wheel (402), the arc-shaped rack (403) is connected and fixed to the equipment seat (1), a connection cover C (404) is rotatably connected to the outer wall of the flushing pipe (4), the connection cover C (404) is threadedly connected to the liquid injection port (601), and a plurality of nozzles (405) are fixedly connected to the flushing pipe (4).

8. A plasma freezing process parameter detection device according to claim 1, characterized in that: The scrubbing mechanism (5) comprises a fixed frame (501), the fixed frame (501) is connected and fixedly arranged with the outer wall of the flushing pipe (4), the fixed frame (501) is provided with two connecting rod groups (502) in a symmetrical structure and rotatably connected, a scrubbing rod (503) is rotatably arranged between the other ends of the two connecting rod groups (502), a spring (504) is connected and fixedly arranged on the scrubbing rod (503), the other end of the spring (504) is connected and fixedly arranged with the inner wall of the fixed frame (501), an electric push rod (505) is connected and fixedly arranged with the inner wall of the fixed frame (501), and an adjusting rack (506) is connected and fixedly arranged at the output end of the electric push rod (505), and an adjusting wheel (507) is connected and fixedly arranged at one end of one of the connecting rod groups (502) connected to the fixed frame (501), and the adjusting wheel (507) is meshed and transmission-arranged with the adjusting rack (506).

9. A method for detecting parameters of plasma freezing process, using a plasma freezing process parameter detection device according to any one of claims 1 to 8, comprising the following steps: S1. First, inject simulated plasma into the simulated plasma barrel (2), and then inject an appropriate amount of simulated plasma into the simulated plasma bag (6) through the control panel (8); S2, then placing the simulated plasma bag (6) inside the plasma quick-freezing machine, and collecting temperature data through the parameter collection mechanism (7); S3, after freezing for 4 hours, take out and read the freezing process curve of the parameter acquisition mechanism (7) through Bluetooth communication. The software will automatically analyze the freezing process curve and calculate the temperature parameters of the freezing process, including the pre-cooling stage time, cooling rate, crystallization stage temperature, crystallization time, deep cooling stage time, cooling rate, minimum temperature and time of supercooling state, temperature deviation of plasma freezer, and temperature uniformity; S4. The simulated plasma in the simulated plasma bag (6) is then poured out after melting, and then the simulated plasma bag (6) is mounted on the rocking seat (3), and the flushing tube (4) is inserted into the simulated plasma bag (6). When the rocking seat (3) swings, the flushing tube (4) and the scrubbing mechanism (5) are driven to rotate, thereby flushing and scrubbing the inner wall of the simulated plasma bag (6).

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