Sample adding method of automatic analysis instrument and automatic analysis instrument

Abstract

The invention provides a sample adding method of an automatic analysis instrument and the automatic analysis instrument. The sample adding method comprises the following steps: dividing cycle time into a time slice s1 to a time slice s6 in sequence; adding a sample to the reaction cup in the first target hole position of the sample needle operation position within the s1 time slice; s2, rotating the first target hole position to a reagent needle operation position in the time slice; s3, adding a reagent into the reaction cup in the first target hole site within the time slice; s4, the first target hole position is rotated to the uniform mixing operation position within the time slice; s5, uniformly mixing the reaction cups in the first target hole position within the time slice, moving the reaction cups which are currently positioned in a second target hole position of the gripper operation position and are uniformly mixed in the previous sample adding process to the incubation plate, and grabbing a new reaction cup and putting the new reaction cup into the second target hole position; and s6, rotating the second target hole position to the sample needle operation position within the time slice, wherein the roles of the first target hole position and the second target hole position are exchanged in the next sample adding process. The automatic analysis instrument adopts the sample adding method. According to the invention, the average time of a single sample adding process can be shortened, and the accuracy of an instrument analysis detection result can be improved.

Description

Technical Field

[0001] The present invention belongs to the technical field of medical devices, and more specifically, relates to a sample adding method of an automatic analysis instrument and the automatic analysis instrument. Background Art

[0002] In the related art, a type of automatic analysis device, such as a chemiluminescent immunoassay analyzer, is usually implemented based on a corresponding sample loading mechanism, which includes a sample loading plate with multiple wells and a gripper operation position, a sample needle operation position, a reagent needle operation position, and a mixing operation position sequentially arranged on the rotation path of the sample loading plate. A sample loading process of the above-mentioned automatic analysis device is specifically as follows: control the gripper to grab a new reaction cup and place it in the target well currently at the gripper operation position; rotate the sample loading plate to move the target well to the sample needle operation position; control the sample to add the sample to the reaction cup in the target well; rotate the sample loading plate to move the target well to the reagent needle operation position; control the reagent to add the reagent to the reaction cup in the target well; rotate the sample loading plate to move the target well to the mixing operation position; control the mixer to mix the reaction cup in the target well; rotate the sample loading plate to move the target well to the gripper operation position; control the gripper to move the reaction cup in the target well to the incubation plate.

[0003] In practical application, the sample addition process of the automatic analysis device mentioned above mainly has the following problems:

[0004] In order to ensure the mixing effect of the sample and reagent in the reaction cup, the mixing operation of the mixer accounts for a large proportion of the time of a single sample addition process, which leads to a long average time of a single sample addition process. In addition, since the mixer uses a method of causing the reaction cup to vibrate slightly to achieve the mixing operation, bubbles will inevitably exist in the liquid in the reaction cup after the mixing operation, and the presence of bubbles will affect the accuracy of the final analysis and detection results of the automatic analysis device to a certain extent. Summary of the invention

[0005] The purpose of the present invention is to solve the problem that the sample adding method of the automatic analysis device in the related art causes the average time of a single sample adding process to be long and affects the accuracy of the analysis and detection results of the automatic analysis device.

[0006] In order to achieve the above object, the present invention provides a sample adding method of an automatic analysis instrument and an automatic analysis instrument.

[0007] According to a first aspect of the present invention, there is provided a sample loading method for an automatic analysis instrument, the sample loading method being implemented based on a sample loading mechanism, the sample loading mechanism comprising a sample loading tray and a gripper operation position, a sample needle operation position, a reagent needle operation position and a mixing operation position sequentially arranged on a rotation path of the sample loading tray;

[0008] The sample adding plate has at least four holes, and the distribution of the holes on the sample adding plate satisfies the condition: if any hole is currently in the mixing operation position, there is another hole currently in the gripper operation position;

[0009] The sample addition method includes adopting a predetermined periodic control strategy for each intermediate sample addition process except the first sample addition process and the last sample addition process;

[0010] The periodic control strategy includes:

[0011] Divide the cycle time into time slices s1 to s6 in sequence;

[0012] In the s1 time slice, the control sample is directed to add the sample to the reaction cup in the first target well position located at the sample needle operation position;

[0013] In the s2 time slice, the sample loading plate is rotated to move the first target well position to the reagent needle operation position;

[0014] In the s3 time slice, the reagent is controlled to add the reagent to the reaction cup in the first target well;

[0015] In the s4 time slice, the sample loading plate is rotated to move the first target well position to the mixing operation position;

[0016] In the s5 time slice, the mixer is controlled to mix the cuvette in the first target well position, the gripper is controlled to move the cuvette in the second target well position currently located at the gripper operation position and which has been mixed in the previous sample loading process to the incubation plate, and a new cuvette is grabbed and placed in the second target well position;

[0017] In the s6 time slice, the sample loading plate is rotated to move the second target well position to the sample needle operation position, and the first target well position and the second target well position exchange roles in the next sample loading process.

[0018] According to a second aspect of the present invention, there is provided an automatic analysis instrument, the automatic analysis instrument comprising a sample loading mechanism, the sample loading mechanism comprising a sample loading tray and a gripper operation position, a sample needle operation position, a reagent needle operation position and a mixing operation position sequentially arranged on a rotation path of the sample loading tray;

[0019] The sample adding plate has at least four holes, and the distribution of the holes on the sample adding plate satisfies the condition: if any hole is currently in the mixing operation position, there is another hole currently in the gripper operation position;

[0020] The automatic analysis instrument further comprises a processor and a memory, and the processor implements a corresponding sample addition method when executing a computer program stored in the memory;

[0021] The sample addition method includes adopting a predetermined periodic control strategy for each intermediate sample addition process except the first sample addition process and the last sample addition process;

[0022] The periodic control strategy includes:

[0023] Divide the cycle time into time slices s1 to s6 in sequence;

[0024] In the s1 time slice, the control sample is directed to add the sample to the reaction cup in the first target well position located at the sample needle operation position;

[0025] In the s2 time slice, the sample loading plate is rotated to move the first target well position to the reagent needle operation position;

[0026] In the s3 time slice, the reagent is controlled to add the reagent to the reaction cup in the first target well;

[0027] In the s4 time slice, the sample loading plate is rotated to move the first target well position to the mixing operation position;

[0028] In the s5 time slice, the mixer is controlled to mix the cuvette in the first target well position, the gripper is controlled to move the cuvette in the second target well position currently located at the gripper operation position and which has been mixed in the previous sample loading process to the incubation plate, and a new cuvette is grabbed and placed in the second target well position;

[0029] In the s6 time slice, the sample loading plate is rotated to move the second target well position to the sample needle operation position, and the first target well position and the second target well position exchange roles in the next sample loading process.

[0030] The beneficial effects of the present invention are:

[0031] The sample adding method of the automatic analysis instrument of the present invention adopts a predetermined periodic control strategy for each intermediate sample adding process except the first sample adding process and the last sample adding process, and the periodic control strategy includes: dividing the cycle time into time slices s1 to s6 in sequence; in the s1 time slice, controlling the sample to add the sample to the reaction cup in the first target well position at the sample needle operation position; in the s2 time slice, rotating the sample adding disk to move the first target well position to the reagent needle operation position; in the s3 time slice, controlling the reagent to add the test sample to the reaction cup in the first target well position; agent; in the s4 time slice, the sample loading plate is rotated to move the first target well position to the mixing operation position; in the s5 time slice, the mixer is controlled to mix the reaction cup in the first target well position, and the gripper is controlled to move the reaction cup in the second target well position that has been mixed in the previous sample loading process and is currently in the gripper operation position to the incubation plate, and a new reaction cup is grabbed and placed in the second target well position; in the s6 time slice, the sample loading plate is rotated to move the second target well position to the sample needle operation position, and the first target well position and the second target well position exchange roles in the next sample loading process.

[0032] According to the sampling method of the automatic analyzer of the present invention, for each sampling process except the last sampling process, the reaction cup that has undergone the mixing operation in the current sampling process will not be transferred to the incubation disk in the current sampling process, but will be transferred to the incubation link after the sample tray continues to rotate a certain distance in the next sampling process. Specifically, the process in which the reaction cup continues to rotate with the sample tray includes two start-stop processes, namely, the first target hole position rotates from the sample needle operation position to the reagent needle operation position and the first target hole position rotates from the reagent needle operation position to the mixing operation position. After each of the above-mentioned start-stop processes is completed, the liquid in the reaction cup will shake more significantly than the mixing operation due to inertia. This phenomenon of liquid shaking will promote further mixing of the liquid in the reaction cup on the one hand, and on the other hand, it is conducive to the dispersion of bubbles in the liquid.

[0033] Based on the above, since the shaking of the liquid will promote further mixing of the liquid in the reaction cup, the mixing operation time of the mixer can be appropriately shortened. Although there will be no reaction cups transferred to the incubation stage in the first loading process, when loading in large quantities, due to the large number of loading processes, when the time of the first loading process is evenly distributed to other loading processes, the average time of a single loading process can still be less than the average time of a single loading process in the related art. At the same time, as the reaction cup that has been mixed by the mixer continues to rotate with the reaction disk, the bubbles in the liquid in the reaction cup will be released to a certain extent, thereby improving the accuracy of the analysis and detection results of the automatic analysis device.

[0034] It can be seen that the sample adding method of the automatic analysis instrument of the present invention can effectively solve the problem that the sample adding method of the automatic analysis device in the related art causes the average time of a single sample adding process to be long and affects the accuracy of the analysis and detection results of the automatic analysis device.

[0035] The automatic analyzer of the present invention and the sample adding method of the automatic analyzer described above belong to a general inventive concept, and have at least the same beneficial effects as the sample adding method of the automatic analyzer described above, and the beneficial effects are not repeated here.

[0036] Other features and advantages of the present invention will be described in detail in the following detailed description. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] The present invention may be better understood by referring to the following description taken in conjunction with the accompanying drawings, in which the same or similar reference numerals are used throughout the drawings to represent the same or similar components.

[0038] Figure 1 A flow chart showing an implementation of a sample addition method of an automatic analysis instrument according to an embodiment of the present invention is shown;

[0039] Figure 2 A schematic structural diagram of a sample loading mechanism according to an embodiment of the present invention is shown. DETAILED DESCRIPTION

[0040] In order to enable those skilled in the art to more fully understand the technical solution of the present invention, the exemplary embodiments of the present invention will be described in more comprehensive and detailed in conjunction with the accompanying drawings below. Obviously, the one or more embodiments of the present invention described below are only one or more of the specific ways in which the technical solution of the present invention can be implemented, and are not exhaustive. It should be understood that other ways belonging to a general inventive concept can be used to implement the technical solution of the present invention, and should not be limited by the exemplary embodiments described. Based on one or more embodiments of the present invention, all other embodiments obtained by ordinary technicians in the field without making creative work should fall within the scope of protection of the present invention.

[0041] Example: Figure 1 The flowchart of the sample adding method of the automatic analysis instrument according to the embodiment of the present invention is shown. Figure 1 The sample loading method of the automatic analysis instrument of the embodiment of the present invention is implemented based on a sample loading mechanism, which includes a sample loading plate and a gripper operation position, a sample needle operation position, a reagent needle operation position and a mixing operation position sequentially arranged on the rotation path of the sample loading plate;

[0042] The sample adding plate has at least four holes, and the hole distribution on the sample adding plate meets the condition: if any hole is currently in the mixing operation position, there is another hole currently in the gripper operation position;

[0043] The above-mentioned sample addition method includes adopting a predetermined periodic control strategy for each intermediate sample addition process except the first sample addition process and the last sample addition process, and the periodic control strategy includes:

[0044] Step S100, dividing the cycle time into time slices s1 to s6 in sequence;

[0045] Step S200, within the time slice s1, control the sample to add the sample to the reaction cup in the first target well position at the sample needle operation position;

[0046] Step S300: within the time slice s2, the sample loading plate is rotated to move the first target well position to the reagent needle operation position;

[0047] Step S400, in the s3 time slice, controlling the reagent to add the reagent to the reaction cup in the first target well;

[0048] Step S500: within the time slice s4, rotating the sample loading plate to move the first target well position to the mixing operation position;

[0049] Step S600: In the s5 time slice, the mixer is controlled to mix the cuvette in the first target well position, the gripper is controlled to move the cuvette in the second target well position that is currently in the gripper operation position and has been mixed in the previous sample addition process to the incubation plate, and a new cuvette is grabbed and placed in the second target well position;

[0050] Step S700: within the time slice s6, the sample loading plate is rotated to move the second target well position to the sample needle operation position, and the first target well position and the second target well position exchange roles in the next sample loading process.

[0051] Specifically, Figure 2 FIG. 2 shows a schematic diagram of the structure of the sample loading mechanism according to an embodiment of the present invention. Figure 2In the embodiment of the present invention, the sample adding plate 100 has 10 holes 110, which are hole positions 1 to 10 respectively; the gripper operation position 200, the sample needle operation position 300, the reagent needle operation position 400 and the mixing operation position 500 are sequentially arranged on the rotation path of the sample adding plate 100, wherein hole position 1 is currently in the gripper operation position 200, hole position 3 is currently in the sample needle operation position 300, hole position 7 is currently in the reagent needle operation position 400, and hole position 9 is currently in the mixing operation position 500. A mixer is arranged under the mixing operation position 500. When the cuvette in the hole position currently in the mixing operation position 500 needs to be mixed, the mixer is first raised to make it contact with the cuvette, and then the mixer is turned on.

[0052] The following is based on Figure 2 The sample adding method of the automatic analysis instrument of the embodiment of the present invention is described in more detail:

[0053] Assume that the current process is the Nth sample loading process. The reaction cup that needs to add samples, reagents, and mixing is placed on the x1 hole on the loading plate, and the new reaction cup is placed on the x2 hole. The position relationship between the x1 hole and the x2 hole is that when the x1 hole is at the mixing operation position, the x2 hole is at the gripper operation position. Then, the relationship is as follows:

[0054] The N-1th sample loading process: the sample, reagent and mixed reaction cup are placed on the sample loading plate in the x2 hole;

[0055] The Nth sample loading process: the reaction cup for adding samples, reagents and mixing on the loading plate is placed on the x1 hole;

[0056] The N+1th sample loading process: add samples, reagents and mix well on the loading plate and place the reaction cup on the x2 hole.

[0057] The specific operations of the above three sample addition processes are as follows:

[0058] N-1th sample addition process:

[0059] In the s1 time slice, the sample target is controlled to add sample to the reaction cup in the x2 hole at the sample needle operation position; in the s2 time slice, the sample adding disk is rotated to move the x2 hole to the reagent needle operation position; in the s3 time slice, the reagent target is controlled to add reagent to the reaction cup in the x2 hole; in the s4 time slice, the sample adding disk is rotated to move the x2 hole to the mixing operation position; in the s5 time slice, the mixer is controlled to mix the reaction cup in the x2 hole, the gripper is controlled to move the reaction cup in the x1 hole to the incubation disk, and a new reaction cup is grabbed and placed in the x1 hole; in the s6 time slice, the sample adding disk is rotated to move the x1 hole to the sample needle operation position.

[0060] Nth sample addition process:

[0061] In the s1 time slice, the sample target is controlled to add sample to the reaction cup in the x1 hole which is at the sample needle operation position; in the s2 time slice, the sample adding plate is rotated to move the x1 hole to the reagent needle operation position; in the s3 time slice, the reagent target is controlled to add reagent to the reaction cup in the x1 hole; in the s4 time slice, the sample adding plate is rotated to move the x1 hole to the mixing operation position; in the s5 time slice, the mixer is controlled to mix the reaction cup in the x1 hole, the gripper is controlled to move the reaction cup in the x2 hole to the incubation plate, and a new reaction cup is grabbed and placed in the x2 hole; in the s6 time slice, the sample adding plate is rotated to move the x2 hole to the sample needle operation position.

[0062] N+1th sample addition process:

[0063] In the s1 time slice, the sample target is controlled to add sample to the reaction cup in the x2 hole at the sample needle operation position; in the s2 time slice, the sample adding disk is rotated to move the x2 hole to the reagent needle operation position; in the s3 time slice, the reagent target is controlled to add reagent to the reaction cup in the x2 hole; in the s4 time slice, the sample adding disk is rotated to move the x2 hole to the mixing operation position; in the s5 time slice, the mixer is controlled to mix the reaction cup in the x2 hole, the gripper is controlled to move the reaction cup in the x1 hole to the incubation disk, and a new reaction cup is grabbed and placed in the x1 hole; in the s6 time slice, the sample adding disk is rotated to move the x1 hole to the sample needle operation position.

[0064] Accordingly, based on the sample adding method of the automatic analyzer of the embodiment of the present invention, the embodiment of the present invention further proposes an automatic analyzer, which includes a sample adding mechanism, which includes a sample adding plate and a gripper operation position, a sample needle operation position, a reagent needle operation position and a mixing operation position which are sequentially arranged on the rotation path of the sample adding plate;

[0065] The sample adding plate has at least four holes, and the hole distribution on the sample adding plate meets the condition: if any hole is currently in the mixing operation position, there is another hole currently in the gripper operation position;

[0066] The above-mentioned automatic analysis instrument also includes a processor and a memory, and the processor implements the corresponding sample addition method when executing the computer program stored in the memory;

[0067] The above-mentioned sample addition method includes adopting a predetermined periodic control strategy for each intermediate sample addition process except the first sample addition process and the last sample addition process;

[0068] The above-mentioned periodic control strategies include:

[0069] Divide the cycle time into time slices s1 to s6 in sequence;

[0070] In the s1 time slice, the control sample is directed to add the sample to the reaction cup in the first target well position at the sample needle operation position;

[0071] In the s2 time slice, the sample loading plate is rotated to move the first target well position to the reagent needle operation position;

[0072] In the s3 time slice, the control reagent is added to the reaction cup in the first target well;

[0073] In the s4 time slice, the sample loading plate is rotated to move the first target well position to the mixing operation position;

[0074] In the s5 time slice, the mixer is controlled to mix the reaction cup in the first target well position, the gripper is controlled to move the reaction cup in the second target well position that has been mixed in the previous sample addition process and is currently in the gripper operation position to the incubation plate, and a new reaction cup is grabbed and placed in the second target well position;

[0075] In the s6 time slice, the sample loading plate is rotated to move the second target well position to the sample needle operation position, and the first target well position and the second target well position exchange roles in the next sample loading process.

[0076] Although one or more embodiments of the present invention are described above, it should be known to those skilled in the art that the present invention can be implemented in any other form without departing from its subject matter and scope. Therefore, the embodiments described above are illustrative rather than restrictive, and many modifications and substitutions are obvious to those skilled in the art without departing from the spirit and scope of the present invention as defined in the appended claims.

Claims

1. A method for adding samples to an automatic analysis instrument, characterized in that: The sample adding method is implemented based on a sample adding mechanism, which includes a sample adding plate and a gripper operation position, a sample needle operation position, a reagent needle operation position and a mixing operation position which are sequentially arranged on a rotation path of the sample adding plate; The sample adding plate has at least four holes, and the distribution of the holes on the sample adding plate satisfies the condition: if any hole is currently in the mixing operation position, there is another hole currently in the gripper operation position; The sample addition method includes adopting a predetermined periodic control strategy for each intermediate sample addition process except the first sample addition process and the last sample addition process; The periodic control strategy includes: Divide the cycle time into time slices s1 to s6 in sequence; In the s1 time slice, the control sample is directed to add the sample to the reaction cup in the first target well position located at the sample needle operation position; In the s2 time slice, the sample loading plate is rotated to move the first target well position to the reagent needle operation position; In the s3 time slice, the reagent is controlled to be added to the reaction cup in the first target well; In the s4 time slice, the sample loading plate is rotated to move the first target well position to the mixing operation position; In the s5 time slice, the mixer is controlled to mix the cuvette in the first target well position, the gripper is controlled to move the cuvette in the second target well position currently located at the gripper operation position and which has been mixed in the previous sample loading process to the incubation plate, and a new cuvette is grabbed and placed in the second target well position; In the s6 time slice, the sample loading plate is rotated to move the second target well position to the sample needle operation position, and the first target well position and the second target well position exchange roles in the next sample loading process.

2. An automatic analysis instrument, characterized in that: The sample adding mechanism comprises a sample adding plate and a gripper operation position, a sample needle operation position, a reagent needle operation position and a mixing operation position which are sequentially arranged on the rotation path of the sample adding plate; The sample adding plate has at least four holes, and the distribution of the holes on the sample adding plate satisfies the condition: if any hole is currently in the mixing operation position, there is another hole currently in the gripper operation position; The automatic analysis instrument further comprises a processor and a memory, and the processor implements a corresponding sample addition method when executing a computer program stored in the memory; The sample addition method includes adopting a predetermined periodic control strategy for each intermediate sample addition process except the first sample addition process and the last sample addition process; The periodic control strategy includes: Divide the cycle time into time slices s1 to s6 in sequence; In the s1 time slice, the control sample is directed to add the sample to the reaction cup in the first target well position located at the sample needle operation position; In the s2 time slice, the sample loading plate is rotated to move the first target well position to the reagent needle operation position; In the s3 time slice, the reagent is controlled to be added to the reaction cup in the first target well; In the s4 time slice, the sample loading plate is rotated to move the first target well position to the mixing operation position; In the s5 time slice, the mixer is controlled to mix the cuvette in the first target well position, the gripper is controlled to move the cuvette in the second target well position currently located at the gripper operation position and which has been mixed in the previous sample loading process to the incubation plate, and a new cuvette is grabbed and placed in the second target well position; In the s6 time slice, the sample loading plate is rotated to move the second target well position to the sample needle operation position, and the first target well position and the second target well position exchange roles in the next sample loading process.

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