Cat drinking water additive as well as preparation method and application thereof

Abstract

The invention discloses a cat drinking water additive as well as a preparation method and application thereof, and the cat drinking water additive comprises a rosemary extract, nepeta lactone and fish hydrolysate. The rosemary extract, the nepeta lactone and the fish hydrolysate are matched according to a specific proportion and have a synergistic effect, and the enthusiasm of the cats to actively drink water can be improved by adding the additive into the drinking water of the cats according to the specific proportion.

Description

Technical Field

[0001] The invention belongs to the field of feed additives, and in particular relates to a cat drinking water additive and a preparation method and application thereof. Background Art

[0002] With the increasing number of pet-raising families, pet health issues are receiving more and more attention from pet owners. However, since cats have an innate fear of water, most cats will not take the initiative to draw water. Lack of water can cause many harms to cats. For example, it may cause cats to become dehydrated, show low spirits, rapid heartbeat, and in severe cases, shock or even death; it may also cause urinary system diseases such as kidney stones, cystitis, and urethritis due to concentrated urine; in addition, it may also cause a series of health problems such as getting angry, constipation, metabolic disorders, dry skin and hair, and decreased resistance. Summary of the invention

[0003] The purpose of the present invention is to provide a cat drinking water additive and a preparation method and application thereof, wherein the additive can enhance the enthusiasm of cats to actively drink water.

[0004] In order to solve the problems existing in the prior art, the technical solution adopted by the present invention is:

[0005] In a first aspect, the present invention provides a cat drinking water additive, comprising rosemary extract, nepetalactone and fish hydrolyzate.

[0006] Furthermore, the solid-liquid ratio of the rosemary extract, nepetalactone and fish hydrolyzate is 1.2-12 g: 26.4-27.6 g: 120 ml.

[0007] Furthermore, the fish meat hydrolyzate comprises the following components: fish meat, immobilized enzymes and water.

[0008] Furthermore, the mass ratio of the fish meat to water is 1:1.5-3, preferably 1:2; the mass of the immobilized enzyme is 0.3%-5% of the mass of the fish meat, preferably 1.6%.

[0009] Furthermore, the immobilized enzyme includes neutral protease and papain, and the mass ratio of the neutral protease to papain is 2:1-2, preferably 2:1.

[0010] Furthermore, it also includes a preservative, and the preservative is nisin.

[0011] Furthermore, the final concentration of the preservative in the fish hydrolyzate is 0.0012-0.006 g / 120 ml, preferably 0.0036 g / 120 ml.

[0012] In a second aspect, the present invention provides a method for preparing the cat drinking water additive described in the first aspect, comprising the following steps:

[0013] S1: Preparation of fish hydrolysate:

[0014] Fresh crucian carp is pretreated, fish meat is taken, mashed, fixed enzyme is added, the initial pH of the substrate is adjusted to 6.0-7.0, the temperature is controlled at 34-38°C, hydrolyzed for 6-8 hours, heated in a boiling water bath for 10-30 minutes to inactivate the enzyme, and then centrifuged at 3000-4000r / min for 15-20 minutes, and the obtained supernatant is the fish meat hydrolyzate;

[0015] S2: After adding the preservative, sterilize in a high pressure steam sterilizer for 30-60 minutes;

[0016] S3: Add rosemary extract and nepetalactone.

[0017] In a third aspect, the present invention provides the use of the cat drinking water additive described in the first aspect or the cat drinking water additive prepared according to the method described in the second aspect in promoting cats to actively draw water.

[0018] Furthermore, the added amount of the cat drinking water additive is: 0.3-2mL / 100mL drinking water.

[0019] The advantages and beneficial effects of the present invention are:

[0020] The invention discloses a cat drinking water additive, which comprises rosemary extract, nepetalactone and fish hydrolyzate. The fish hydrolyzate, rosemary extract and nepetalactone are mixed in a specific ratio to have a synergistic effect. The additive is added into cat drinking water in the specific ratio to enhance the enthusiasm of the cat to drink water actively. DETAILED DESCRIPTION

[0021] The technical solutions in the embodiments of the present application are clearly described below. Obviously, the described embodiments are part of the embodiments of the present application, rather than all of the embodiments. All other embodiments obtained by ordinary technicians in this field based on the embodiments in the present application belong to the scope of protection of the present application.

[0022] The experimental methods in the following examples, unless otherwise specified, are all conventional methods, and are performed according to the techniques or conditions described in the literature in the field or according to the product instructions. The materials, reagents, etc. used in the following examples, unless otherwise specified, can all be obtained from commercial channels.

[0023] Experimental Example 1

[0024] 1 Test method

[0025] 1.1 Test materials

[0026] 1.1.1 Test reagents

[0027] Nepetalactone was purchased from Xi'an Ruihe Bioengineering Technology Co., Ltd.; rosemary extract was purchased from Zhengzhou Kaizhiyu Food Additive Co., Ltd.; papain was purchased from Linying County Xiangmanyi Trading Co., Ltd.; neutral protease was purchased from Linying County Xiangmanyi Trading Co., Ltd.; agar powder was purchased from Henan Wanbang Chemical Technology Co., Ltd.; nisin was purchased from Shandong Yuantai Bioengineering Co., Ltd.; chitosan was purchased from Henan Gaobao Industrial Co., Ltd.

[0028] 1.1.2 Culture medium

[0029] NA medium was purchased from Hangzhou Tianhe Microbiological Reagent Co., Ltd.

[0030] 1.1.3 Test equipment

[0031] 100mL measuring cylinder, 90mm culture dish, 50mL centrifuge tube, 1000mL conical flask, 100μl pipette tip, glass rod, applicator, marker pen, medicine spoon, weighing paper, etc.

[0032] 1.1.4 Main instruments and equipment

[0033] Electronic balance, Sartorius Scientific Instruments (Beijing) Co., Ltd.; low-speed refrigerated centrifuge, Wuxi Ruijiang Analytical Instrument Co., Ltd.; portable pressure steam sterilizer, Shanghai Huaxian Medical Nuclear Instrument Co., Ltd.; clean bench, Suzhou Antai Air Technology Co., Ltd.; pipette, Thermo, USA; electric blast drying oven, Shanghai Testing Instrument Factory Co., Ltd.

[0034] 1.1.5 Experimental animals

[0035] There are 5 Chinese rural cats. Cat A is 1.75kg, female, 5 months old; Cat B is 1.75kg, male, 5 months old; Cat C is 1.25kg, female, 4 months old; Cat D is 1.40kg, male, 4 months old; Cat E is 2.30kg, male, 8 months old.

[0036] 1.2 Screening of preservatives

[0037] 1.2.1 Preparation of test solution

[0038] Fresh crucian carp was pretreated and the fish meat was minced. The fixed enzyme was composed of neutral protease and papain in a mass ratio of 2:1, the mass of the fixed enzyme was 1.6% of the mass of the fish meat, the mass ratio of the fish meat to water was 1:2, the initial pH of the substrate was adjusted to 7.0, the temperature was controlled at 37°C, and the hydrolysis was carried out for 7 hours. The enzyme was inactivated by heating in a boiling water bath for 10 minutes, and then centrifuged at 4000r / min for 15 minutes to obtain the supernatant. The volume of the supernatant was measured, and 0.6g of rosemary extract and 12g of nepetalactone were added to each 120mL of the supernatant to obtain the test solution.

[0039] The prepared test liquid was divided into three groups, each with two portions, each of which was No. 1 and No. 2. The first group was the control group, which did not add any preservative, the second group added 0.0036 g / 120 ml of nisin, and the third group added 1.8 g / 120 ml of chitosan. After preparation, all liquids were sterilized in a high-pressure steam sterilizer for 30 minutes.

[0040] 1.2.2 Evaluation of preservation effect

[0041] The three groups of solutions were stored at 4°C, and sensory evaluation and microbiological examination were performed on agent No. 1 every 7 days. After the examination, liquid No. 1 was exposed to the air for 10 seconds to examine the effects of different preservatives on the shelf life of the product after opening.

[0042] Reagent No. 2 was subjected to a long-term test, i.e. the three groups of liquids were sealed and placed at 4°C for 6 months, with samples taken every 30 days for sensory evaluation and microbiological investigation.

[0043] 1.2.3 Sensory evaluation

[0044] The sensory evaluation criteria are shown in Table 1:

[0045] Table 1 Sensory evaluation standards

[0046]

[0047] 1.2.4 Microbiological investigation

[0048] (1) Preparation of NA medium

[0049] Take 500 mL of distilled water in a conical flask, then use an electronic balance to weigh 16.5 g of NA culture medium powder and add it to the conical flask. Stir with a glass rod until the powder is evenly dissolved in the water. After high-pressure steam sterilization for 30 minutes, pour into a flat plate, and after solidification, turn it upside down and set aside.

[0050] (2) Bacterial culture

[0051] Spread plate method: sterile operation, after mixing each group of test solutions, take 0.1mL and spread it on NA culture medium, set 3 replicates for each group of test solutions, and take 0.1mL of distilled water and spread it on NA culture medium as blank control.

[0052] Operation method: First, gently push the bacterial solution back and forth along a straight line to make it evenly distributed, then change the direction 90° and push it back and forth along another vertical line. The direction at the inner edge of the plate can be changed and spread several times with a coating rod. After standing at room temperature for 5 to 10 minutes, invert and culture at 37°C for 48 hours.

[0053] (3) Calculation of total colony count

[0054] Calculate the average number of colonies on the plate within the appropriate counting range, and then multiply the average by 10 to obtain the total number of colonies per milliliter of sample.

[0055] 1.3 Ratio screening

[0056] 1.3.1 Preparation of fish hydrolysate

[0057] Fresh crucian carp was pretreated, the fish meat was taken and mashed. The fixed enzyme was composed of neutral protease and papain in a mass ratio of 2:1, the mass of the fixed enzyme was 1.6% of the mass of the fish meat, the mass ratio of the fish meat to water was 1:2, the initial pH of the substrate was adjusted to 7.0, the temperature was controlled at 37°C, and the hydrolysis was carried out for 7 hours. The enzyme was inactivated by heating in a boiling water bath for 10 minutes, and then centrifuged at 4000r / min for 15 minutes. The obtained supernatant was added with the best preservative screened by the above test and then sterilized by high-pressure steam, which was the final fish meat hydrolyzate.

[0058] 1.3.2 Methods for observing the effects of additives

[0059] (1) Two bowl test

[0060] Take two bowls of the same specifications, put 100 mL of pure water in each bowl, add a certain amount of sample to one of the small bowls, and make sure that the amount of water provided in the bowl is equal to or slightly higher than the cat's daily drinking water. Place the test cat in the middle of the two bowls and allow it to eat and drink freely within the predetermined time (20 minutes). The test time is scheduled for the morning and evening after half a day of water deprivation. Record the bowl from which the cat first drinks water, and measure the remaining liquid in the bowl after the predetermined time. Record the consumption of the two bowls of liquid. The difference in drinking water reflects the test cat's preference for liquid odor and palatability. During the test, the positions of the two bowls need to be exchanged from time to time to eliminate the influence of the position of the test bowl on the attractiveness of the cat.

[0061] (2) Olfactory test

[0062] Add samples of different proportions to 100 mL of pure water to obtain different test solutions. Use two test solutions of different proportions in one test and put them in two small bowls of the same specifications so that the test cat can fully smell the odor of the two liquids. The two small bowls are placed at a certain distance apart, and the test cat is placed in the middle between the two bowls so that it can freely choose the small bowl containing the test solution. Record the test solution that the test cat chooses for the first time, take the cat away from the small bowl, exchange the small bowl position, repeat the above operation, record the data, and repeat the test.

[0063] 1.3.3 Determination of ingredient content

[0064] (1) Determination of the optimal concentration of rosemary extract

[0065] Take 250mL of the prepared fish hydrolyzate, divide it into five parts, add 5g of nepetalactone to each part, add different masses of rosemary extract thereto respectively, and obtain test samples with rosemary extract concentrations of 0.01g / mL, 0.025g / mL, 0.05g / mL, 0.075g / mL, and 0.1g / mL, respectively. Add the test samples of different concentrations to 100mL of pure water and compare them with an equal amount of pure water for olfactory test to observe the minimum concentration that attracts cats.

[0066] (2) Determination of the optimal concentration of nepetalactone

[0067] Take 250 mL of the prepared fish hydrolyzate, add the rosemary extract of the optimal concentration obtained in the above test, divide it into five parts, divide the two concentrations obtained in the preliminary test into five groups with a gradient of 0.01 g / mL, add different masses of nepetalactone into them, and perform olfactory tests in pairs from low to high concentrations. Repeat each group of tests 2-3 times, and record the test results.

[0068] (3) Determination of the palatability of drinking water additives

[0069] Based on the above experiments, a relatively low concentration that is highly attractive to cats was selected as the final ratio of the drinking water additive. 0.1-2mL of the drinking water additive was added to 100mL of pure water, and two bowls were tested with an equal amount of pure water, and the test results were recorded.

[0070] 2 Test results

[0071] 2.1 Screening test of commonly used preservatives

[0072] The short-term test results are shown in Table 2, the sensory evaluation results are shown in Table 3, and the long-term test results are shown in Table 4:

[0073] Table 2

[0074]

[0075] Table 3

[0076]

[0077]

[0078] Table 4

[0079]

[0080] In the short-term test, a colony count of 0 indicates that the reagent is not contaminated, and a higher sensory evaluation score indicates that the added ingredients perform better. Tables 2 and 3 show that the reagent with added nisin can be stored at 4°C for about 3 weeks after opening. During this period, the appearance, diffusion, and odor of the reagent group did not change significantly, and the viscosity increased slightly, which is speculated to be related to water evaporation and low-temperature storage; the chitosan group can be stored for about a week, and the appearance and viscosity have obvious changes in the later period. After three weeks, there is an obvious fishy smell and a glass rod is needed to stir to spread evenly; the control group was contaminated with bacteria within a week, and the sensory evaluation score was the lowest among the three groups.

[0081] In long-term tests, a colony count of 0 indicates that the reagent is not contaminated. As shown in Table 4, as time goes by, the lactic acid group has been contaminated by bacteria since the third month of cultivation, while the chitosan and control groups have been contaminated by bacteria within two months, and the number has increased significantly over time. In addition, in the same period of time, the deterioration and contamination of the other two groups is significantly stronger than that of the lactic acid group.

[0082] Based on the comprehensive short-term and long-term test results, it was found that the lactic acid group deteriorated and was the slowest to become contaminated, and had a more outstanding preservation effect.

[0083] 2.2 Matching screening test

[0084] 2.2.1 Results of determination of optimum concentration of rosemary extract

[0085] In the olfactory test, the samples with rosemary extract concentrations of 0.01g / mL, 0.025g / mL, 0.05g / mL, 0.075g / mL, and 0.1g / mL were more attractive to cats than pure water, and no colonies were produced in the five groups of samples during the test. "√" is the cat selection result, and " / " is an invalid result. Each group of tests was repeated twice. The specific results are shown in Tables 5-9:

[0086] Table 5

[0087]

[0088]

[0089] Table 6

[0090]

[0091] Table 7

[0092]

[0093] Table 8

[0094]

[0095]

[0096] Table 9

[0097]

[0098] It can be seen from Tables 5 to 9 that the five groups of concentrations of the tested samples have better attraction and better antibacterial effect than pure water, so the lowest concentration, i.e. 0.01 g / mL, was selected as the final addition concentration of rosemary extract.

[0099] 2.2.2 Results of determination of optimal concentration of nepetalactone

[0100] In the preliminary test, the attraction to cats was 0.25g / mL≈0, 20g / mL>0.30g / mL>0.15g / mL>0.35g / mL>0.10g / mL, so according to the results of the preliminary test, 0.20-0.24g / mL nepetalactone was selected for olfactory test, and the concentration of rosemary extract was 0.01g / mL. "√" is the cat selection result, " / " is an invalid result, each group of tests was repeated twice, and the results are shown in Tables 10-15:

[0101] Table 10

[0102]

[0103] Table 11

[0104]

[0105] Table 12

[0106]

[0107] Table 13

[0108]

[0109] Table 14

[0110]

[0111] Table 15

[0112]

[0113] It can be seen from Tables 10 to 15 that 0.22 g / mL and 0.23 g / mL are the most attractive to cats. Taking comprehensive cost considerations into account, 0.22 g / mL was finally selected as the final added concentration of nepetalactone.

[0114] 2.2.3 Results of palatability test of additives

[0115] The test measured the palatability of the solution when 2mL, 1mL, 0.5mL, 0.3mL, and 0.1mL of the additive were added to 100mL of pure water. The concentration of nepetalactone in the additive was 0.22g / mL. It was compared with pure water. The data recorded in the table is the average of the amount of water (mL) drunk by cats in three tests, where " / " is invalid data. The results are shown in Tables 16-20:

[0116] Table 16

[0117]

[0118] Table 17

[0119]

[0120] Table 18

[0121]

[0122] Table 19

[0123]

[0124] Table 20

[0125]

[0126] It can be seen from Tables 16-20 that, within the range of 0.3-2 mL of dripping amount per 100 mL, although some cats will ignore the existence of beverages and choose pure water, most cats are more willing to drink beverages with the additive of the present application. After repeated tests, almost all cats choose to drink beverages; and in the test group with a dripping amount of 0.1 mL per 100 mL, cats chose to drink pure water more often, and chose pure water after sniffing between the two bowls, which was not attractive. Therefore, the best dripping amount is 0.3-2 mL per 100 mL.

[0127] In summary, nisin has a better preservation effect on the reagent after opening. When preparing, 0.01g / mL rosemary extract and 0.22g / mL nepetalactone are selected as the final composition concentration of the drinking water additive; when using, 0.3-2mL of the drinking water additive is added to every 100mL of drinking water to achieve a good attraction effect.

[0128] Example 1

[0129] This embodiment provides a cat drinking water additive, including 120 ml of fish hydrolyzate, 1.2 g of rosemary extract and 26.4 g of nepetalactone.

[0130] The fish meat hydrolysate is composed of 60g crucian carp meat, fixed enzyme, 0.0036g nisin and 120ml water, wherein the fixed enzyme is composed of 0.64g neutral protease and 0.32g papain. The fish meat may also be other species of fish meat.

[0131] The preparation method of the cat drinking water additive described in the present embodiment comprises the following steps:

[0132] S1: Preparation of fish hydrolysate:

[0133] Fresh crucian carp was pretreated, the fish meat was taken, mashed, neutral protease and papain were added, and then water was added to adjust the initial pH of the substrate to 7.0, the temperature was controlled at 37°C, and the hydrolysis was performed for 7 hours. The enzyme was inactivated by heating in a boiling water bath for 10 minutes, and then centrifuged at 4000r / min for 15 minutes to obtain the supernatant.

[0134] S2: Add nisin to the supernatant and sterilize in a high-pressure steam autoclave for 30 minutes to obtain fish hydrolyzate.

[0135] S3: 1.2 g of rosemary extract and 26.4 g of nepetalactone were added to 120 ml of fish hydrolyzate.

[0136] Example 2

[0137] This embodiment provides a cat drinking water additive. The only difference between this embodiment and Example 1 is that the amount of rosemary extract used is 12g, and the rest is the same as Example 1.

[0138] Comparative Example 1

[0139] This comparative example provides a cat drinking water additive. The difference between this comparative example and Example 1 is that clove extract is used instead of rosemary extract, and the rest is the same as Example 1.

[0140] Comparative Example 2

[0141] This comparative example provides a cat drinking water additive. The only difference between this comparative example and Example 1 is that it does not contain nepetalactone. The additive in this comparative example only consists of 120 ml of fish hydrolyzate and 1.2 g of rosemary extract, and the rest is the same as Example 1.

[0142] Comparative Example 3

[0143] This comparative example provides a cat drinking water additive. The only difference between this comparative example and Example 1 is that the additive does not contain rosemary extract. The additive of this comparative example only consists of 120 ml of fish hydrolyzate and 26.4 g of nepetalactone, and the rest is the same as Example 1.

[0144] Experimental Example 2

[0145] 1 Test methods

[0146] 1.1 Experimental animals

[0147] There are 8 cats. Cat 1 is a Chinese rural cat, 2.8kg, female, 8 months old; Cat 2 is a Chinese rural cat, 3.95kg, male, 1 year old; Cat 3 is a Chinese rural cat, 3.65kg, male, 2 years old; Cat 4 is a Ragdoll cat, 4.45kg, female, 2 years old; Cat 5 is a Chinese rural cat, 4.22kg, male, 3 years old; Cat 6 is a British shorthair cat, 4.05kg, male, 2 years old; Cat 7 is a British shorthair cat, 3.55kg, female, 2 years old; Cat 8 is a Chinese rural cat, 1.55kg, female, 4 months old.

[0148] 1.2 Experimental Grouping:

[0149] Group 1: cat drinking water additive prepared in Example 1;

[0150] Group 2: cat drinking water additive prepared in Example 2;

[0151] Group 3: cat drinking water additive prepared in Comparative Example 1;

[0152] Group 4: cat drinking water additive prepared in Comparative Example 2;

[0153] Group 5: cat drinking water additive prepared in Comparative Example 3;

[0154] The above five reagents were subjected to olfactory tests in pairs, and each group of tests was repeated 2-3 times, and the test results were recorded.

[0155] 1.3 Olfactory test

[0156] Add the additives of groups 1-5 to 100mL of pure water at a ratio of 1ml / 100ml to obtain five test solutions. Use two different test solutions in one test and put them in two small bowls of the same specifications so that the test cat can fully smell the odor of the two liquids. The two small bowls are placed at a certain distance apart, and the test cat is placed in the middle between the two bowls so that it can freely choose the small bowl containing the test solution. Record the test solution that the test cat chooses for the first time, take the cat away from the small bowl, exchange the small bowl position, repeat the above operation, record the data, and repeat the test.

[0157] 2 Experimental results

[0158] "√" is the cat selection result, " / " is an invalid result, each group of experiments was repeated twice, and the results are shown in Table 21-Table 24:

[0159] Table 21

[0160]

[0161] Table 22

[0162]

[0163]

[0164] Table 23

[0165]

[0166] Table 24

[0167]

[0168] As shown in Table 21, the cat drinking water additives prepared in Examples 1 and 2 both have a strong inducing effect on cats drinking water, but the inducing effect of Example 1 is better. It is speculated that because the rosemary concentration in Example 2 is higher, it masks the taste of nepetalactone that is more attractive to cats, so the inducing ability of Example 2 for cats is lower than that of Example 1.

[0169] It can be seen from Table 22 that since clove extract is used instead of rosemary extract in Comparative Example 1, the clove extract cannot produce a synergistic effect with the fish hydrolyzate and nepetalactone, and is not attractive to cats. Almost no cats smell it, and most cats choose the cat drinking water additive of Example 1.

[0170] As shown in Table 23, since Comparative Example 2 does not contain nepetalactone, the fish hydrolyzate and rosemary extract cannot produce a synergistic effect and are not attractive to cats. Most cats choose the cat drinking water additive of Example 1.

[0171] As shown in Table 24, since Comparative Example 3 does not contain rosemary extract, the fish hydrolyzate and nepetalactone cannot produce a synergistic effect and are not attractive to cats. Most cats choose the cat drinking water additive of Example 1.

[0172] It can be seen from Tables 21-24 that adding nepetalactone and rosemary extract in a specific proportion to the specially prepared fish hydrolyzate has the greatest attraction to cats, indicating that the special fish hydrolyzate, nepetalactone and rosemary extract used in a specific proportion have a synergistic effect on inducing cats to drink water, and the additive of the present invention can enhance the enthusiasm of cats to drink water actively.

[0173] The embodiments of the present application are described above, but the present application is not limited to the above-mentioned specific implementation modes. The above-mentioned specific implementation modes are merely illustrative and not restrictive. Under the guidance of the present application, ordinary technicians in this field can also make many forms without departing from the purpose of the present application and the scope of protection of the claims, all of which are within the protection of the present application.

Claims

1. A cat drinking water additive, characterized in that: Includes rosemary extract, nepetalactone and fish hydrolyzate.

2. A cat drinking water additive according to claim 1, characterized in that: The solid-to-liquid ratio of the rosemary extract, nepetalactone and fish hydrolyzate is 1.2-12:26.4-27.6:

120.

3. A cat drinking water additive according to claim 1 or 2, characterized in that: The fish meat hydrolyzate comprises the following components: fish meat, immobilized enzymes and water.

4. A cat drinking water additive according to claim 3, characterized in that: The mass ratio of the fish meat to water is 1:1.5-3; the mass of the fixed enzyme is 0.3%-5% of the mass of the fish meat.

5. A cat drinking water additive according to claim 4, characterized in that: The fixed enzymes include neutral protease and papain, and the mass ratio of the neutral protease to the papain is 2:1-2.

6. A cat drinking water additive according to claim 3, characterized in that: The invention also comprises a preservative, wherein the preservative is nisin.

7. A cat drinking water additive according to claim 6, characterized in that: The final concentration of the preservative in the fish hydrolyzate is 0.0012-0.006 g / 120 mL.

8. A method for preparing a cat drinking water additive according to any one of claims 1 to 7, characterized in that: The following steps are involved: S1: Preparation of fish hydrolysate: Fresh crucian carp is pretreated, fish meat is taken, mashed, fixed enzyme is added, the initial pH of the substrate is adjusted to 6.0-7.0, the temperature is controlled at 34-38°C, hydrolyzed for 6-8 hours, heated in a boiling water bath for 10-30 minutes to inactivate the enzyme, and then centrifuged at 3000-4000r / min for 15-20 minutes, and the obtained supernatant is the fish meat hydrolyzate; S2: After adding the preservative, sterilize in a high pressure steam sterilizer for 30-60 minutes; S3: Add rosemary extract and nepetalactone.

9. Use of a cat drinking water additive according to any one of claims 1 to 7 or a cat drinking water additive prepared according to the method of claim 8 in promoting cats to actively draw water.

10. The use according to claim 9, characterized in that: The added amount of the cat drinking water additive is: 0.3-2mL / 100mL drinking water.

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