Health-care product capable of enhancing bone mineral density and preparation method of health-care product

Abstract

The invention discloses a health-care product capable of enhancing bone mineral density and a preparation method of the health-care product. The health-care product is prepared from the following raw materials in parts by weight: 10-30 parts of morinda citrifolia ferment powder, 5-15 parts of sunflower seed extract, 10-20 parts of organic acid calcium, 1-5 parts of a sweetening agent, 20-40 parts of lactose and 10-30 parts of microcrystalline cellulose. The morinda citrifolia ferment powder, the sunflower seed extract and the organic acid calcium are used as functional components, and from multiple mechanisms of regulating intestinal steady state, promoting osteoblast proliferation and differentiation, inhibiting osteoclast activity, promoting calcium absorption, relieving oxidative stress, inhibiting inflammation and the like, continuous increase of bone mineral density is effectively promoted, and osteoporosis is prevented and treated. According to the health-care product, morinda citrifolia and sunflower seeds which are homologous in medicine and food are used as raw materials, the components are natural, safety is high, and the requirements of different people for increasing bone mineral density and preventing and treating osteoporosis can be met.

Description

Technical Field

[0001] The invention belongs to the field of health care products, and in particular relates to a health care product capable of enhancing bone density and a preparation method thereof. Background Art

[0002] Osteoporosis is a common metabolic bone disease, which is mainly characterized by decreased bone density and bone destruction, which can easily lead to fractures and dysfunction. The etiology and pathogenesis of osteoporosis is a complex process involving genetic factors, endocrine disorders, malnutrition, lifestyle and other aspects. The following are some common etiologies and pathogenesis: Genetic factors play a role in the onset of osteoporosis. Some people may have hereditary osteoporosis susceptibility genes, making them more susceptible to osteoporosis. Disorders of the endocrine system may lead to the occurrence of osteoporosis. Women are more likely to develop osteoporosis after menopause, which is due to the decrease in estrogen levels, which leads to increased bone resorption and reduced bone formation. Endocrine diseases such as hypothyroidism and adrenal insufficiency are also associated with osteoporosis. Lack of nutrients such as calcium and vitamin D can lead to osteoporosis. Calcium is the main component of bones, and vitamin D helps absorb and utilize calcium. If the intake of calcium and vitamin D is insufficient, the bones cannot be fully nourished, thereby increasing the risk of osteoporosis. An unhealthy lifestyle is also associated with osteoporosis. Bad habits such as lack of exercise, long-term bed rest, alcoholism, smoking, etc. increase the risk of osteoporosis. Other factors may also lead to the occurrence of osteoporosis, such as long-term use of certain drugs (such as hormone drugs, anti-epileptic drugs, etc.), chronic inflammatory diseases, renal insufficiency, etc.

[0003] At present, the prevention and treatment of osteoporosis mainly focuses on two aspects: one is to increase the intake of relevant nutrients during adolescence so that the optimal bone peak can be achieved during bone maturity; the other is to take appropriate measures to slow down bone loss as much as possible before and after menopause. Health foods with the function of "increasing bone density" on the market mostly contain mineral elements such as calcium, zinc, zinc, vitamins such as vitamin D, proteins or amino acids such as casein phosphopeptides, active ingredients of traditional Chinese medicine such as puerarin, and other functional ingredients. These ingredients achieve the purpose of increasing bone density by supplementing calcium or by adjusting endocrine to promote calcium absorption, but there are still certain defects, such as safety issues. Studies have shown that long-term intake of vitamin D is prone to poisoning when the blood concentration is greater than 500nmol / L, which manifests as nausea, anorexia, fatigue, etc., and even renal failure in severe cases. In addition, large amounts of calcium supplementation can easily cause high blood calcium levels, which can increase the risk of cardiovascular disease. Casein phosphopeptides are easily destroyed by gastrointestinal enzymes in the stomach, and it is often necessary to increase the dosage of casein phosphopeptides, which can easily cause side effects such as allergies. For example, the patent with publication number CN 101559218 A discloses a composition with the function of increasing bone density, which is composed of the following components: calcium carbonate, glucosamine hydrochloride, chondroitin sulfate, casein phosphopeptide and soy isoflavones. The composition contains phytoestrogen soy isoflavones and is not suitable for calcium supplementation for children, pregnant and lactating women, gynecological tumor patients and those with a family history of gynecological tumors.

[0004] Therefore, it is necessary to develop a health product that can enhance bone density from medicinal and edible materials with high safety, good taste, and suitable for different groups of people to meet the needs of consumers. Summary of the invention

[0005] The purpose of the present invention is to provide a health product capable of enhancing bone density and a preparation method thereof in view of the deficiencies of the prior art. The health product has the effect of significantly enhancing bone density, has low side effects, and has good flavor, and can meet the needs of different groups of people for preventing and treating osteoporosis.

[0006] To achieve the above purpose, the technical solution adopted by the present invention is as follows:

[0007] A health product capable of enhancing bone density comprises the following raw materials in parts by weight: 10-30 parts of noni fruit fermentation powder, 5-15 parts of sunflower seed extract, 10-20 parts of organic acid calcium, 1-5 parts of sweetener, 20-40 parts of lactose and 10-30 parts of microcrystalline cellulose.

[0008] Preferably, the health product capable of enhancing bone density comprises the following raw materials in parts by weight: 20 parts of noni fruit fermentation powder, 10 parts of sunflower seed extract, 15 parts of organic acid calcium, 3 parts of sweetener, 30 parts of lactose and 22 parts of microcrystalline cellulose.

[0009] Preferably, the preparation steps of the noni fruit fermentation powder are: washing fresh noni fruit and soaking it in a complex enzyme solution for 4 to 8 hours, then drying and crushing it, placing the noni fruit powder in a solid fermentation reactor, fermenting and ripening it at 45 to 55° C. for 24 to 72 hours to obtain ripe noni fruit powder; adding oligosaccharides and probiotics liquid to the ripe noni fruit powder, fermenting it at 35 to 45° C. for 6 to 9 days to obtain noni fruit fermentation product; drying the noni fruit fermentation product, crushing it, and sieving it to obtain noni fruit fermentation powder.

[0010] Preferably, the concentration of the complex enzyme solution is 1-2% m / v, and is composed of cellulase and amylase in a mass ratio of 2:1; the mass ratio of the ripened noni fruit powder, oligosaccharides and probiotic bacterial liquid is 100:10:1.

[0011] Preferably, the oligosaccharide is one or more of galacto-oligosaccharide, fructo-oligosaccharide and isomalto-oligosaccharide; the concentration of the probiotic solution is 1.0×10 6 ~1.5×10 6 cfu / mL, and the probiotics are lactic acid bacteria or bifidobacteria.

[0012] Preferably, the preparation steps of the sunflower seed extract are: fresh, mold-free sunflower kernels are defatted by extrusion, the defatted kernels are dried and crushed, sieved, protease and cellulase are added for enzymolysis, the enzymolyzed kernel powder is dispersed in a sodium acetate aqueous solution, exposed to a high-voltage pulsed electromagnetic field for 10 to 15 minutes at room temperature, then heated to 55 to 60°C and kept still for 3 to 4 hours, filtered, the filtrate is taken, and the filtrate is dried to obtain the sunflower seed extract.

[0013] Preferably, the amount of the protease used is 0.1% to 0.3% by weight of the defatted kernel powder; the amount of the cellulase used is 0.1% to 0.5% by weight of the defatted kernel powder.

[0014] Preferably, the concentration of the sodium acetate aqueous solution is 0.5-2 mol / L.

[0015] Preferably, the organic acid calcium is calcium gluconate, calcium lactate or vitamin C calcium.

[0016] Preferably, the sweetener is one or more of stevioside, trehalose, maltitol and sucrose.

[0017] The present invention also provides a method for preparing the above-mentioned health care product capable of enhancing bone density, comprising the following steps: uniformly mixing noni fruit fermentation powder, sunflower seed extract, organic acid calcium, sweetener, lactose and microcrystalline cellulose, adding an appropriate amount of ethanol solution to prepare a soft material, sieving granules, drying, and sieving whole granules to obtain the health care product.

[0018] Noni fruit is a fruit that can be used as both medicine and food. It is rich in various nutrients, including various antioxidant active substances and bioactive ingredients such as vitamins, amino acids, minerals, trace elements, polyphenols, flavonoids, alkaloids and glycoside compounds. It is known as the "magic fruit" and "super fruit" and has multiple functions such as anti-oxidation, detoxification, enhancing immunity, improving digestion and beauty. Related studies have pointed out that the bioactive ingredients of noni fruit have a positive effect on postmenopausal women and have potential anti-osteoporosis effects. However, when the noni fruit matures, it emits a unique odor, also known as "cheese fruit" and "vomiting fruit", which limits the acceptance of the product. The present invention first pre-treats fresh noni fruit by soaking it in a complex enzyme solution, then uses noni fruit endogenous bacteria to perform solid-state fermentation and ripening treatment, and then adds oligosaccharides and probiotic liquid for fermentation treatment. The unpleasant smell of the noni fruit fermentation powder obtained is significantly weakened, the taste is improved, and the content of nutrients including phenolic substances, flavonoids, organic acids, amino acids, etc. is significantly improved, especially the content of antioxidant components (such as superoxide dismutase and glutathione reductase) is significantly increased, which greatly improves its application value. The noni fruit endogenous bacteria, probiotics, and antioxidant components contained in the noni fruit fermentation powder can regulate bone metabolism and increase bone density by changing the immune status of the whole body and bone marrow. Facts have proved that compared with noni fruit enzyme powder and noni fruit extract prepared by conventional processes, the noni fruit fermentation powder provided by the present invention has more excellent effects in flavor, nutritional value, and increasing bone density.

[0019] Sunflower seeds are a kind of plant kernels rich in fatty acids, proteins and minerals. Compared with other plant kernels, the level of chlorogenic acid contained in them is higher. The present invention uses the sunflower seed meal obtained after de-oiling as a raw material to extract bioactive peptides and chlorogenic acid, and adds protease and cellulase for enzymatic pretreatment to promote the degradation of proteins into polypeptides and effectively degrade the fiber structure. The enzymatically hydrolyzed seed kernel powder is dispersed in a sodium acetate aqueous solution with a pH of about 9.2 to 9.5. Under the action of a pulsed electromagnetic field, the osmotic extraction effect of the weakly alkaline sodium acetate aqueous solution is enhanced, chlorogenic acid is effectively released, and polypeptides are further promoted to be degraded into small molecule bioactive peptides. The sunflower seed extract prepared by the method of the present invention contains a high level of bioactive peptides and chlorogenic acid, which acts on the skeletal system through multiple mechanisms such as promoting osteoblast proliferation and differentiation, inhibiting osteoclast activity, improving calcium absorption and deposition, regulating bone metabolism-related gene expression, and promoting collagen synthesis, thereby effectively increasing bone density and improving bone health. Moreover, its oil content is very low, and it is not easy to oxidize and deteriorate, which helps to improve the storage stability of health products.

[0020] Compared with ordinary calcium (calcium phosphate, calcium carbonate, etc.), organic acid calcium is easily absorbed, has high bioavailability, has little irritation to the gastrointestinal tract, and does not have the risk of causing hypercalcemia or increasing kidney stones.

[0021] The present invention uses Noni fruit fermented powder, sunflower seed extract, and organic acid calcium as effective ingredients, and effectively promotes the continuous growth of bone density and prevents and treats osteoporosis from multiple mechanisms such as regulating intestinal homeostasis, promoting osteoblast proliferation and differentiation, inhibiting osteoclast activity, promoting calcium absorption, reducing oxidative stress, and inhibiting inflammation. With the addition of appropriate amounts of sweeteners and fillers, a granular health product is made, which is not only convenient to take, but also has a good taste. The health product of the present invention uses Noni fruit and sunflower seeds, which are both medicinal and edible, as raw materials, has natural ingredients, high safety, and can meet the needs of different populations to increase bone density and prevent and treat osteoporosis. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 These are the results of the determination of total phenolic and total flavonoid contents and antioxidant enzyme (SOD, GR) activities of noni fruit fermented powder (NF-1, NF-2), noni fruit enzyme powder (NE) and noni fruit extract (NW and NA).

[0023] Figure 2 The results of the bone density increasing function test of the health food of the present invention. Among them, A is the femoral bone density test result; B is the tartrate acid phosphatase 5b (TRACP5b) content test result; C is the type I collagen cross-linked C-terminal peptide (CTX) content test result. Compared with the sham operation group (S), ### p<0.001; compared with the model group (M), * p<0.05, ** p<0.01, *** p<0.001. DETAILED DESCRIPTION

[0024] The following examples are provided to further illustrate the present invention, rather than to limit the present invention.

[0025] Example 1

[0026] (1) Preparation of Noni Fruit Fermented Powder:

[0027] Fresh noni fruit was washed and soaked in a 1% m / v complex enzyme solution (cellulase: amylase = 2:1, mass ratio) for 4 hours, then dried and crushed; noni fruit powder was placed in a solid fermentation reactor and fermented and ripened at 55°C for 24 hours to obtain ripe noni fruit powder; 10 g of oligosaccharide, 1 g of lactic acid bacteria solution (1.0×10 6cfu / mL), and fermented in a solid fermentation reactor at 40°C for 6 days to obtain a noni fruit fermentation product; the noni fruit fermentation product was dried at 40°C, crushed, and sieved to obtain a noni fruit fermentation powder (NF-1).

[0028] (2) Preparation of Noni Fruit Fermentation Powder:

[0029] Fresh noni fruit was washed and soaked in a 2% m / v complex enzyme solution (cellulase: amylase = 2:1, mass ratio) for 8 hours, then dried and crushed; noni fruit powder was placed in a solid fermentation reactor and fermented and ripened at 45°C for 72 hours to obtain ripe noni fruit powder; 10 g of oligosaccharide, 1 g of lactic acid bacteria solution (1.5×10 6 cfu / mL), and fermented in a solid fermentation reactor at 40°C for 9 days to obtain a noni fruit fermentation product; the noni fruit fermentation product was dried at 40°C, crushed, and sieved to obtain a noni fruit fermentation powder (NF-2).

[0030] (3) Preparation of Noni fruit enzyme powder: fresh Noni fruit is washed, dried, and crushed to a diameter of 0.5 cm. At the same time, 0.2% pectinase and 0.2% cellulase by weight of the Noni fruit are added, and the mixture is stirred into a tank. The temperature is maintained at 30°C. After fermentation for 2 months, the Noni mash in the tank is stirred and separated into seeds, skins and pulp by a single-channel pulper. The pulp is squeezed to separate the Noni pulp and Noni stock solution. The Noni stock solution is stored at low temperature and aged for half a year to obtain Noni fruit enzyme stock solution. The Noni fruit enzyme stock solution is freeze-dried to obtain Noni fruit enzyme powder (NE).

[0031] (4) Preparation of Noni fruit extract (hot water ultrasound-assisted extraction): Fresh Noni fruit was washed, sliced, freeze-dried, and crushed to a particle size of 10-30 μm to obtain Noni fruit powder; the Noni fruit powder was mixed with water in a mass ratio of 1:20, the pH was adjusted to 5.2, and ultrasound-assisted extraction was performed at 80°C for 90 min, the ultrasound frequency was 20 kHz, the ultrasound power was 100 mW / mL, and the mixture was centrifuged at 5000 rpm for 10 min. The supernatant was collected and freeze-dried to obtain Noni fruit water extract (NW).

[0032] (5) Preparation of Noni fruit extract (ethanol immersion extraction): Fresh Noni fruit was washed, sliced, freeze-dried, and crushed to a particle size of 10-30 μm to obtain Noni fruit powder; the Noni fruit powder was mixed with a 75% ethanol solution in a mass ratio of 1:20, immersed at 50°C for 6 h, centrifuged at 5000 rpm for 10 min, the supernatant was collected, and freeze-dried to obtain Noni fruit alcohol extract (NA).

[0033] The total phenolic and total flavonoid contents of Noni fruit fermented powder NF-1, NF-2, Noni fruit enzyme powder NE, Noni fruit water extract NW, Noni fruit alcohol extract NA prepared by the above method, as well as the activities of superoxide dismutase (SOD) and glutathione reductase (GR) were determined respectively. Figure 1 .

[0034] Depend on Figure 1 It can be seen that compared with the noni fruit enzyme powder (NE) and noni fruit extract (NW, NA) prepared by conventional processes, the total phenol and total flavonoid contents of the noni fruit fermentation powder (NF-1, NF-2) of the present invention are significantly increased, and the activities of SOD and GR are significantly improved, which helps to enhance the effect of noni fruit fermentation powder in increasing bone density and preventing and treating osteoporosis.

[0035] Example 2

[0036] (1) Preparation of sunflower seed extract:

[0037] Fresh, mold-free sunflower kernels are defatted by extrusion, the defatted kernels are dried and crushed, sieved, 0.3% protease by weight and 0.5% cellulase by weight of the defatted kernel powder are added, and enzymolysis is carried out at 50°C for 2 hours. The enzymolysis kernel powder is dispersed in a 1 mol / L sodium acetate aqueous solution at a solid-liquid ratio of 1 g:10 mL, and exposed to a high-voltage pulsed electromagnetic field for 15 minutes at room temperature, with the field intensity of the pulsed electromagnetic field controlled at 20 kv / cm and the frequency of 20 Hz, and then heated to 55°C and kept standing for 4 hours, filtered, and the filtrate is taken, and the filtrate is dried to obtain a sunflower seed extract (SSE1).

[0038] (2) Preparation of sunflower seed extract (conventional process):

[0039] Fresh, mold-free sunflower kernels are defatted by extrusion, the defatted kernels are dried and crushed, sieved, and the defatted kernel powder is dispersed in water at a solid-liquid ratio of 1 g:10 mL, soaked at 65°C for 2 h, cooled to 50°C, 0.3% protease by weight of the defatted kernel powder is added, enzymatically hydrolyzed at 50°C for 4 h, centrifuged at 2500 r / min for 10 min, the supernatant is taken, and the supernatant is dried to obtain sunflower seed extract (SSE2).

[0040] The yields of polypeptides and chlorogenic acid in the sunflower seed extracts (SSE1, SSE2) prepared by the above method were determined respectively. The results are shown in Table 1.

[0041] Table 1 Yields of peptides and chlorogenic acid in sunflower seed extract

[0042] Group Peptide yield (%) Chlorogenic acid yield (%) Process of the present invention 39.8 7.7 Conventional process 10.1 3.5

[0043] As can be seen from Table 1, compared with the sunflower seed extract prepared by conventional processes, the sunflower seed extract of the present invention has higher contents of polypeptides and chlorogenic acid, which helps to enhance the effect of the sunflower seed extract in increasing bone density and preventing and treating osteoporosis.

[0044] Example 3

[0045] A health product capable of enhancing bone density comprises the following raw materials in parts by weight: 20 parts of noni fruit fermentation powder (NF-2), 10 parts of sunflower seed extract (SSE1), 15 parts of calcium lactate, 3 parts of stevioside, 30 parts of lactose and 22 parts of microcrystalline cellulose.

[0046] Preparation steps: mix noni fruit fermentation powder, sunflower seed extract, calcium lactate, stevioside, lactose and microcrystalline cellulose evenly, add appropriate amount of ethanol solution to prepare soft material, granulate through 18-mesh sieve, dry at 60°C, and granulate through 16-mesh sieve to obtain the product.

[0047] Example 4

[0048] A health product capable of enhancing bone density comprises the following raw materials in parts by weight: 10 parts of noni fruit fermentation powder (NF-2), 15 parts of sunflower seed extract (SSE1), 10 parts of calcium lactate, 1 part of stevioside, 40 parts of lactose and 24 parts of microcrystalline cellulose.

[0049] The preparation steps are as in Example 3.

[0050] Example 5

[0051] A health product capable of enhancing bone density comprises the following raw materials in parts by weight: 30 parts of noni fruit fermentation powder (NF-2), 5 parts of sunflower seed extract (SSE1), 10 parts of calcium lactate, 5 parts of maltitol, 40 parts of lactose and 10 parts of microcrystalline cellulose.

[0052] The preparation steps are as in Example 3.

[0053] Example 6

[0054] A health product capable of enhancing bone density comprises the following raw materials in parts by weight: 15 parts of noni fruit fermentation powder (NF-2), 10 parts of sunflower seed extract (SSE1), 20 parts of calcium gluconate, 5 parts of maltitol, 20 parts of lactose and 30 parts of microcrystalline cellulose.

[0055] The preparation steps are as in Example 3.

[0056] Example 7

[0057] A health product capable of enhancing bone density comprises the following raw materials in parts by weight: 20 parts of noni enzyme powder (NE), 10 parts of sunflower seed extract (SSE1), 15 parts of calcium lactate, 3 parts of stevioside, 30 parts of lactose and 22 parts of microcrystalline cellulose.

[0058] The preparation steps are as in Example 3.

[0059] Example 8

[0060] A health product capable of enhancing bone density comprises the following raw materials in parts by weight: 20 parts of noni fruit water extract (NW), 10 parts of sunflower seed extract (SSE1), 15 parts of calcium lactate, 3 parts of stevioside, 30 parts of lactose and 22 parts of microcrystalline cellulose.

[0061] The preparation steps are as in Example 3.

[0062] Example 9

[0063] A health product capable of enhancing bone density comprises the following raw materials in parts by weight: 20 parts of noni fruit alcohol extract (NA), 10 parts of sunflower seed extract (SSE1), 15 parts of calcium lactate, 3 parts of stevioside, 30 parts of lactose and 22 parts of microcrystalline cellulose.

[0064] The preparation steps are as in Example 3.

[0065] Example 10

[0066] A health product capable of enhancing bone density comprises the following raw materials in parts by weight: 20 parts of noni fruit fermentation powder (NF-2), 10 parts of sunflower seed extract (SSE2), 15 parts of calcium lactate, 3 parts of stevioside, 30 parts of lactose and 22 parts of microcrystalline cellulose.

[0067] The preparation steps are as in Example 3.

[0068] Comparative Example 1

[0069] A health product capable of enhancing bone density comprises the following raw materials in parts by weight: 10 parts of sunflower seed extract (SSE1), 15 parts of calcium lactate, 3 parts of stevioside, 45 parts of lactose and 27 parts of microcrystalline cellulose.

[0070] The preparation steps are as in Example 3.

[0071] Comparative Example 2

[0072] A health product capable of enhancing bone density comprises the following raw materials in parts by weight: 20 parts of noni fruit fermentation powder (NF-2), 15 parts of calcium lactate, 3 parts of stevioside, 40 parts of lactose and 22 parts of microcrystalline cellulose.

[0073] The preparation steps are as in Example 3.

[0074] Comparative Example 3

[0075] A health product capable of enhancing bone density comprises the following raw materials in parts by weight: 15 parts of calcium lactate, 3 parts of stevioside, 50 parts of lactose and 32 parts of microcrystalline cellulose.

[0076] The preparation steps are as in Example 3.

[0077] Application Example 1: Acute Toxicity Test

[0078] According to the "Safety Toxicological Evaluation Standards for Health Foods" (2003 Edition), the maximum tolerated dose method (MTD) was used for testing, specifically: 90 ICR mice weighing 18 to 22g were selected, half male and half female, and randomly divided into Example 3-10 group and control group, with 10 mice in each group. Among them, the mice in Example 3-10 group were gavaged with the health products prepared in Example 3-10 at a dose of 25g / kg, and the mice in the control group were gavaged with an equal volume of normal saline, gavaged twice a day for 14 consecutive days, and the behavior and signs of mice in each group were observed, and the poisoning manifestations and deaths were recorded. The results showed that the growth of mice in each group was good, no symptoms of poisoning reactions were observed, no deaths were observed, and the hematological and physical and chemical indexes were normal. The results showed that the maximum tolerated dose of the health products of the present invention for acute import tests on mice was greater than 25g / kg, and belonged to the non-toxic level.

[0079] Application Example 2: Increase bone density function detection

[0080] 130 SPF-grade SD female rats weighing 250-320g were selected. 120 of them were subjected to ovariectomy, and the remaining 10 were subjected to sham surgery. Five days after the ovariectomy of the rats, vaginal images were examined to confirm that the rats' ovaries were completely removed. The 120 ovariectomized rats were randomly divided into 12 groups, 10 in each group, namely the model group (M), the embodiment 3-10 group (T3-T10) and the comparative example 1-3 group (D1-D3). The 10 rats subjected to sham surgery were set as the sham operation group (S). Among them, the embodiment 3-10 group and the comparative example 1-3 group were gavaged with the health products prepared in the embodiments 3-10 and the comparative example 1-3, respectively, with a gavage dose of 1g / kg, once in the morning and evening each day. The sham operation group and the model group were gavaged with an equal volume of distilled water. Each group of rats was gavaged continuously for 90 days. The femoral bone density of rats in each group was measured by DPX-L dual-energy X-ray absorptiometry, and the levels of tartrate acid phosphatase 5b (TRACP5b) and type I collagen cross-linked C-terminal peptide (CTX) in serum of rats in each group were measured by enzyme-linked immunosorbent assay. Figure 2 .

[0081] Depend on Figure 2It can be seen that the health products prepared in Examples 3-6 of the present invention can significantly increase the femoral bone density of rats, and the effect is better than the health products prepared in Examples 7-10 and Comparative Examples 1-3. It can be seen from Examples 7-9 that compared with Noni fruit enzyme powder (NE), Noni fruit water extract (NW) and Noni fruit alcohol extract (NA), the Noni fruit fermentation powder (NF-2) of the present invention has a better effect on increasing bone density. It can be seen from Example 10 that compared with the sunflower seed extract (SSE2) prepared by conventional processes, the sunflower seed extract (SSE1) of the present invention has a better effect on increasing bone density. It can be seen from Comparative Examples 1-3 that the lack of one or two components of the Noni fruit fermentation powder (NF-2) and sunflower seed extract (SSE1) of the present invention will significantly reduce the effect of the health product on increasing bone density. The results show that the Noni fruit fermentation powder and sunflower seed extract of the present invention have a synergistic effect in increasing bone density.

[0082] In addition, by detecting the levels of TRACP5b and CTX, which are markers of bone resorption, it can be seen that the health care product of the present invention can effectively inhibit the activity of osteoclasts and inhibit bone resorption, and has a good application prospect in preventing and treating osteoporosis.

[0083] The above are only preferred embodiments of the present invention. It should be noted that the above preferred embodiments should not be regarded as limiting the present invention, and the protection scope of the present invention should be based on the scope defined by the claims. For ordinary technicians in this technical field, several improvements and modifications can be made without departing from the spirit and scope of the present invention, and these improvements and modifications should also be regarded as the protection scope of the present invention.

Claims

1. A health product capable of enhancing bone density, characterized in that: The preparation comprises the following raw materials in parts by weight: 10-30 parts of noni fruit fermentation powder, 5-15 parts of sunflower seed extract, 10-20 parts of organic acid calcium, 1-5 parts of sweetener, 20-40 parts of lactose and 10-30 parts of microcrystalline cellulose.

2. The health product capable of enhancing bone density according to claim 1, characterized in that: The preparation steps of the noni fruit fermentation powder are as follows: washing fresh noni fruit, soaking it in a complex enzyme solution for 4-8 hours, drying it, crushing it, placing noni fruit powder in a solid fermentation reactor, fermenting and ripening it at 45-55° C. for 24-72 hours to obtain ripe noni fruit powder; adding oligosaccharides and probiotics liquid to the ripe noni fruit powder, fermenting it at 35-45° C. for 6-9 days to obtain noni fruit fermentation product; drying the noni fruit fermentation product, crushing it, sieving it, and obtaining noni fruit fermentation powder.

3. The health product capable of enhancing bone density according to claim 2, characterized in that: The concentration of the complex enzyme solution is 1-2% m / v, and it is composed of cellulase and amylase in a mass ratio of 2:1; the mass ratio of the ripened noni fruit powder, oligosaccharides and probiotic bacterial liquid is 100:10:

1.

4. The health product capable of enhancing bone density according to claim 2, characterized in that: The oligosaccharide is one or more of galacto-oligosaccharide, fructo-oligosaccharide and isomalto-oligosaccharide; the concentration of the probiotic solution is 1.0×10 6 ~1.5×10 6 cfu / mL, and the probiotics are lactic acid bacteria or bifidobacteria.

5. The health product capable of enhancing bone density according to claim 1, characterized in that: The preparation steps of the sunflower seed extract are as follows: fresh, mildew-free sunflower kernels are defatted by extrusion, the defatted kernels are dried and crushed, sieved, protease and cellulase are added for enzymolysis, the enzymolyzed kernel powder is dispersed in a sodium acetate aqueous solution, exposed to a high-voltage pulse electromagnetic field for 10 to 15 minutes at room temperature, then heated to 55 to 60° C. and kept still for 3 to 4 hours, filtered, the filtrate is taken, and the filtrate is dried to obtain the sunflower seed extract.

6. The health product capable of enhancing bone density according to claim 5, characterized in that: The dosage of the protease is 0.1% to 0.3% by weight of the defatted kernel powder; the dosage of the cellulase is 0.1% to 0.5% by weight of the defatted kernel powder.

7. The health product capable of enhancing bone density according to claim 5, characterized in that: The concentration of the sodium acetate aqueous solution is 0.5-2 mol / L.

8. The health product capable of enhancing bone density according to claim 1, characterized in that: The organic acid calcium is calcium gluconate, calcium lactate or vitamin C calcium.

9. The health product capable of enhancing bone density according to claim 1, characterized in that: The sweetener is one or more of stevioside, trehalose, maltitol and sucrose.

10. A method for preparing a health product capable of enhancing bone density according to any one of claims 1 to 9, characterized in that: The method comprises the following steps: mixing noni fruit fermentation powder, sunflower seed extract, organic acid calcium, sweetener, lactose and microcrystalline cellulose uniformly, adding a proper amount of ethanol solution to prepare a soft material, sieving granules, drying, and sieving whole granules to obtain the product.

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