Citron extract sleeping mask and preparation method thereof

Abstract

The invention relates to the technical field of masks, in particular to a citron extract sleeping mask and a preparation method of the citron extract sleeping mask. 15-25 parts of an intelligent moisturizing system; 3-8 parts of a brain wave synergistic sleep-aiding compound; 8-12 parts of a skin barrier repair system; 2-5 parts of a texture conditioning agent; 0.5 to 1.5 parts of an anticorrosion system; 0.1 to 0.5 part of a fragrance system; according to the present invention, the dual targeting delivery system is developed, the fat-soluble d-limonene is wrapped in the liposome, and the water-soluble naringin is delivered through the nano-micelle; the phospholipid bilayer structure of the liposome is similar to the skin cuticle, so that the transdermal absorption of d-limonene can be promoted. The nano-micelle realizes stable embedding and slow release of the water-soluble flavone through a hydrophilic shell and a hydrophobic core of the nano-micelle. By means of the design, the percutaneous absorption efficiency of the active ingredients is improved, and time-sequence-controllable release is achieved.

Description

Technical Field

[0001] The invention relates to the technical field of facial masks, in particular to a citron extract sleeping facial mask and a preparation method thereof. Background Art

[0002] As people's understanding of the relationship between sleep quality and skin health deepens, sleeping masks have attracted widespread attention as a new skin care product. Citron, as a member of the citrus family, has become a hot topic in skin care product research due to its unique biological activity. However, the existing technology still has many shortcomings in the use of citron extracts.

[0003] Traditional applications of citron extracts mainly focus on their antioxidant and whitening effects. Some studies have shown that flavonoids rich in citron peel, such as naringin and neohesperidin, have significant free radical scavenging ability. On the other hand, d-limonene in citron essential oil has been shown to have certain soothing and aromatic effects. However, these studies are often limited to the exploration of a single effect, ignoring the diverse potential of citron extracts.

[0004] In the prior art, some products have tried to apply citron extract to sleeping masks, but they mainly focus on its refreshing fragrance to enhance the user experience. This application method fails to fully utilize the comprehensive efficacy of citron extract. For example, some products simply add citron essential oil to the mask matrix, but due to the lack of an effective delivery system, its active ingredients are difficult to penetrate the skin and take effect. Although other products use water-extracted citron flavonoids, they fail to solve the stability problem of its water-soluble components in the oil-in-water emulsion system.

[0005] In addition, the prior art also has limitations in the preparation method of citron extract. Conventional water extraction or organic solvent extraction methods often find it difficult to balance the extraction efficiency and the retention of active ingredients. In particular, for thermosensitive compounds in citron, traditional high-temperature extraction may lead to loss of their activity. At the same time, active ingredients of different polarities (such as fat-soluble d-limonene and water-soluble flavonoids) often need to be extracted separately, which not only increases production costs, but may also lead to the loss of certain synergistic effects.

[0006] In terms of product efficacy design, most existing citron extract sleeping masks only provide basic moisturizing and simple soothing effects. They lack in-depth consideration of the physiological mechanism of sleep and cannot effectively improve the sleep quality of users. At the same time, these products often ignore the repair and strengthening of the skin barrier and cannot provide comprehensive night care for the skin. Summary of the invention

[0007] The present invention aims to solve the above problems by innovatively combining citron extract with advanced delivery systems, smart response materials and multi-sensory stimulation to develop a multifunctional and efficient sleeping mask.

[0008] The object of the present invention is to provide a citron extract sleeping mask, which comprises the following components by weight:

[0009] 5-15 parts of citron extract complex;

[0010] Intelligent Moisturizing System 15-25 copies;

[0011] 3-8 servings of Brainwave Synergy Sleep Aid Complex;

[0012] Skin Barrier Repair System 8-12 parts;

[0013] 2-5 parts of texture regulator;

[0014] 0.5-1.5 parts of anticorrosion system;

[0015] Fragrance system 0.1-0.5 parts;

[0016] 30-60 parts of purified water.

[0017] Preferably, the citron extract complex comprises:

[0018] 2-5 parts of citron essential oil extracted by supercritical CO2;

[0019] 3-10 parts of water-extracted citronella flavonoids complex.

[0020] Preferably, the intelligent moisturizing system comprises:

[0021] 5-8 parts of thermosensitive sodium hyaluronate;

[0022] 3-5 parts of plant-derived squalane;

[0023] 7-12 parts of polar seaweed fermented extract.

[0024] Preferably, the brainwave synergistic sleep-aiding complex comprises:

[0025] 1-3 parts of L-theanine;

[0026] 1-3 parts of γ-aminobutyric acid;

[0027] Citral 0.5-1 part.

[0028] Preferably, the skin barrier repair system comprises:

[0029] 3-5 parts of ceramide complex;

[0030] 2-3 portions of vegetable cholesterol;

[0031] 2-3 parts of squalene;

[0032] 1-2 parts of ceramide precursor complex.

[0033] Preferably, the texture regulator comprises:

[0034] Hydroxypropyl methylcellulose 1-2 parts;

[0035] Carbomer 0.5-1.5 parts;

[0036] Glycerin 0.5-1.5 parts.

[0037] Preferably, the anti-corrosion system comprises:

[0038] Phenoxyethanol 0.3-0.8 parts;

[0039] Ethylhexylglycerin 0.1-0.4 parts;

[0040] 0.1-0.3 parts of citric acid.

[0041] Preferably, the fragrance system comprises:

[0042] Citron essential oil 0.05-0.2 parts;

[0043] 0.03-0.15 parts of cedar essential oil;

[0044] 0.02-0.15 parts of frankincense essential oil.

[0045] The preparation method of the citron extract sleeping mask comprises the following steps:

[0046] (1) First, add 70-80 parts by weight of purified water into a main mixing tank and start stirring at a speed of 300-400 rpm; then add 1,3-propylene glycol, glycerin, panthenol, and niacinamide in sequence and stir evenly to obtain an aqueous phase;

[0047] (2) Next, slowly sprinkle hydroxypropyl methylcellulose into purified water at 40-50°C, stir quickly until fully dispersed, and let stand for 1-2 hours to allow it to fully hydrate;

[0048] (3) Again, dissolve L-theanine and γ-aminobutyric acid in purified water at 40-45°C and stir until completely dissolved;

[0049] (4) Then, plant-derived squalane, squalene, ceramide complex, and plant-derived cholesterol are mixed and heated to 60-65° C. to completely melt the mixture, thereby obtaining an oil phase;

[0050] (5) Next, the aqueous phase obtained in step (1) is heated to 60-65° C., and the oil phase obtained in step (4) is slowly added to the aqueous phase under stirring at 600-800 rpm, and homogenized for 5-10 minutes at a homogenization speed of 3000-4000 rpm;

[0051] (6) Further, the mixture obtained in step (5) is cooled to 45-50° C., and the hydroxypropyl methylcellulose and carbomer pre-dispersed in step (2) are added and stirred evenly; the mixture is further cooled to 40° C., and the temperature-sensitive sodium hyaluronate and polar seaweed fermentation extract are added and gently stirred;

[0052] (7) Subsequently, at 35-40° C., add the citron extract complex, the active ingredient solution obtained in step (3), and the ceramide precursor complex in sequence and stir evenly;

[0053] (8) Afterwards, adjust the pH to 5.8-6.2 with 20% citric acid solution;

[0054] (9) Next, add the premixed flavor and preservative at 30-35°C and stir gently for 5-10 minutes;

[0055] (10) Finally, the mixture was degassed at a vacuum of -0.05 MPa to -0.08 MPa for 15–20 min and then filled into pre-sterilized sealed containers in a Class 100 clean room.

[0056] Preferably, the preparation method of the citron extract complex comprises:

[0057] (a) crushing the dried citron peel into 40-60 mesh, loading into an extraction tank, and extracting with supercritical CO2 at 40-50° C. and 20-25 MPa, with a CO2 flow rate of 20-30 kg / h and an extraction time of 2-3 hours to obtain citron essential oil;

[0058] (b) adding 8-10 times the weight of purified water to the residue of step (a), and extracting under reflux at 80-90° C. for 2-3 times, each time for 1-1.5 hours;

[0059] (c) combining the extracts, concentrating under reduced pressure to a relative density of 1.10-1.15 (60° C.), adding 3-5 times the volume of 95% ethanol, and allowing to stand for 12-24 hours;

[0060] (d) filtering, recovering ethanol from the filtrate under reduced pressure, concentrating the filtrate to a paste, washing the paste with an equal volume of ethyl acetate three times, and discarding the ethyl acetate layer;

[0061] (e) The aqueous layer was purified by macroporous adsorption resin column chromatography, eluted with 50% ethanol, and the eluate was concentrated under reduced pressure and spray-dried to obtain citronella flavonoids complex powder.

[0062] The innovative features and technical effects of the present invention are mainly reflected in the following aspects:

[0063] First, the present invention uses a method combining supercritical CO2 extraction with water extraction to achieve efficient extraction of active ingredients of different polarities from citron. Supercritical CO2 extraction can efficiently extract fat-soluble terpenes such as d-limonene under low temperature conditions, avoiding the loss of heat-sensitive components. Subsequent water extraction can effectively obtain water-soluble flavonoids. This combined extraction method not only improves the overall extraction efficiency, but also retains the potential synergistic effect between various active ingredients.

[0064] Secondly, the present invention develops a dual-targeted delivery system, encapsulating fat-soluble d-limonene in liposomes, while water-soluble naringin is delivered through nano-micelles. The phospholipid bilayer structure of liposomes is similar to the stratum corneum of the skin, which can promote the transdermal absorption of d-limonene. Nano-micelles achieve stable embedding and sustained release of water-soluble flavonoids through their hydrophilic shell and hydrophobic core. This design not only improves the transdermal absorption efficiency of the active ingredients, but also achieves time-controlled release.

[0065] Thirdly, the present invention introduces the concept of intelligent moisturizing network, and utilizes the phase change characteristics of thermosensitive sodium hyaluronate at different temperatures to realize the function of dynamically regulating water release according to skin temperature. The N-isopropylacrylamide unit grafted on the side chain of thermosensitive sodium hyaluronate forms intermolecular hydrogen bonds at low temperatures, causing the polymer to shrink; when it is close to skin temperature, the hydrogen bonds are destroyed, the polymer chain stretches, and more water is released. This temperature-dependent conformational change realizes the function of intelligently regulating water release.

[0066] In addition, the present invention creatively incorporates the brainwave synergistic sleep-aiding complex into the mask formula. L-theanine, as an analog of glutamate, can bind to glutamate receptors in the brain and promote the release of the inhibitory neurotransmitter GABA. γ-aminobutyric acid directly acts as an inhibitory neurotransmitter to promote relaxation and sleep by enhancing the activity of GABAergic neurons. Citral activates olfactory receptors, affects the limbic system, and then regulates emotions and promotes relaxation.

[0067] Finally, the present invention pays special attention to the repair and strengthening of the skin barrier in the formula design. By simulating the composition of the skin's natural barrier lipids and combining it with the ceramide precursor complex, it can not only effectively repair the damaged skin barrier, but also significantly reduce transepidermal water loss, providing long-term protection for skin health.

[0068] This multi-level and multi-angle innovative design enables the present invention to show significant advantages in improving sleep quality and skin condition. In particular, the unexpected discovery in terms of skin microecological regulation and circadian rhythm balance provides a new research direction for the development of functional skin care products. The present invention not only solves the problems existing in the existing citron extract sleeping mask, but also creates a new paradigm for functional skin care products, which is expected to open up new sub-segments in the skin care market and meet the needs of modern consumers for efficient and multifunctional skin care products. DETAILED DESCRIPTION

[0069] The thermosensitive sodium hyaluronate of the present invention has a molecular weight of 500-700 kDa and a thermosensitive transition point of 32-34°C.

[0070] Preparation method: Dissolve 1 part by weight of sodium hyaluronate (molecular weight 1-1.5MDa) in 100 parts by weight of deionized water and stir evenly. Add 0.5-1 part by weight of N-isopropylacrylamide monomer and 0.05-0.1 part by weight of ammonium persulfate initiator, react at 40°C for 4-6 hours under nitrogen protection. After the reaction, precipitate the product with ethanol, centrifuge and vacuum dry to obtain thermosensitive sodium hyaluronate. Plant-derived squalane; Source: sugarcane fermentation; Purity: ≥98%, Trade name: Manufacturer: Amyris, Inc.; polar algae fermentation extract, active ingredient: ice-binding proteins content ≥ 1%, total protein content: 15-20%, preparation method: polar algae (Pseudoalteromonas sp.) is inoculated into a culture medium containing 2% glucose, 0.5% yeast extract, and 3% NaCl, and fermented at 4-6°C for 72-96 hours. The supernatant is collected by centrifugation, concentrated by ultrafiltration (molecular weight cutoff 10kDa), and spray-dried to obtain a powder, trade name: Manufacturer: Lipotec (Lubrizol); Ceramide complex (3-5 parts by weight), composition: ceramide NP (30-40%), ceramide AP (20-30%), ceramide EOP (15-25%), total ceramide content: ≥90%, trade name: Manufacturer: Evonik; Plant-based cholesterol, source: soybeans; purity: ≥95%; trade name: PhytoSterol TM , Manufacturer: BTSA Biotecnologías Aplicadas S.L.; Squalene, from: olive oil, purity: ≥98%, trade name: Manufacturer: AAK; cedarwood essential oil, trade name: AtlasCedarwoodOil, manufacturer: Givaudan, frankincense essential oil, trade name: FrankincenseOil manufacturer: Symrise; niacinamide, purity: ≥99.5%, trade name: NiacinamidePC, manufacturer: DSM. Ceramide precursor complex (1-2 parts by weight), composition: palmitoylserine (40-50%), N-acetyl-L-serine (30-40%), total active content: ≥90%, trade name: Manufacturer: Sederma (Croda International Plc).

[0071] Product characteristics and mechanism of action of the present invention:

[0072] Dual targeted delivery system: The essential oil components (mainly d-limonene) in the citron extract complex are encapsulated in liposomes and can penetrate the stratum corneum to reach the dermis. The water-extracted flavonoids (such as naringin and neohesperidin) are slowly released in the epidermis through nano-micelles. This dual delivery system achieves time-controlled release of active ingredients, improving the utilization rate and duration of action of the effective ingredients.

[0073] Intelligent Moisturizing Network: Thermosensitive sodium hyaluronate is in a gel state below 32°C, and turns into a liquid state after contacting the skin (about 32-34°C), achieving temperature-triggered moisture release. Plant-derived squalane and glacier protein in polar seaweed fermentation extract form a bionic sebum membrane structure to further lock in moisture. This intelligent moisturizing system can dynamically adjust moisture release according to skin temperature and ambient humidity to achieve long-lasting moisturizing.

[0074] Brainwave synergistic sleep-aiding mechanism: L-theanine can promote the generation of alpha brain waves and help to relax. γ-aminobutyric acid (GABA), as an inhibitory neurotransmitter, can relieve anxiety and improve sleep quality. Citral activates the limbic system through olfactory stimulation, promotes the secretion of serotonin, and then converts it into melatonin, forming a complete sleep-aiding chain reaction.

[0075] Skin barrier repair system: Ceramide complex (NP, AP, EOP) is combined with plant cholesterol and squalene in a specific ratio to simulate the composition of the skin's natural barrier lipids. Ceramide precursor complex (palmitoylserine, N-acetyl-L-serine) can be absorbed by skin cells and converted into ceramide, further strengthening the barrier function. This system can effectively repair damaged skin barriers and reduce transepidermal water loss.

[0076] Multiple antioxidant synergistic effects: Flavonoids in citron extract (such as naringin and neohesperidin) have strong antioxidant capacity. Together with niacinamide and panthenol, they form a multi-level antioxidant network that can effectively remove free radicals and reduce oxidative stress damage to the skin.

[0077] Intelligent texture control: Through the synergistic effect of hydroxypropyl methylcellulose and carbomer, non-Newtonian fluid properties are achieved. It presents a gel structure when it is static, and becomes liquid when shear force is applied (such as smearing). It quickly returns to the gel state after the shearing stops. This intelligent thixotropic texture is easy to apply and can form a long-lasting protective film on the skin surface.

[0078] Multi-sensory synergistic relaxation: Citron essential oil, cedar essential oil and frankincense essential oil in the fragrance system form a rich aroma, which activates the brain's limbic system through olfactory stimulation and promotes relaxation. At the same time, the unique texture changes provide a pleasant tactile experience, and the visual moisturizing effect can also bring comfort. The synergistic effect of multiple senses helps relieve stress and create a good sleeping atmosphere.

[0079] Biological rhythm regulation: Based on the circadian rhythm theory, the active ingredients in this formula (such as GABA) may help regulate the skin's circadian rhythm and optimize the nighttime repair process by affecting the expression of biological clock genes (such as CLOCK and PER1) in skin cells.

[0080] This multi-dimensional, multi-target formula design achieves synergistic effects among the components. By combining advanced delivery systems, intelligent response materials and bioactive ingredients, it can not only provide deep moisturizing, repair skin barrier, and antioxidant protection, but also regulate the nervous system and improve sleep quality, thus achieving the dual effects of skin care and sleep improvement.

[0081] The citron extract sleeping mask of the present invention exhibits a unique compatibility mechanism and a number of unexpected technical effects:

[0082] The present invention cleverly combines multiple active ingredients in citron extract in a synergistic way. As a terpene compound, d-limonene not only has good percutaneous absorption, but also can enhance the penetration of other active ingredients. Flavonoids such as naringin provide strong antioxidant capacity. The synergistic effect of this fat-soluble and water-soluble ingredients embodies the "complementary principle" in the design of cosmetic formulas.

[0083] By adopting a dual targeted delivery system, the present invention solves the problem of the coexistence of water-soluble and fat-soluble ingredients in traditional O / W emulsification systems. The application of liposomes and nano-micelles not only improves the stability of the formula, but also achieves targeted delivery of active ingredients, reflecting the "nano-carrier technology" in cosmetic physics.

[0084] The introduction of thermosensitive sodium hyaluronate represents a cutting-edge development in cosmetic raw material chemistry. This intelligent polymer material can adjust its structure according to changes in ambient temperature, achieving dynamic regulation of the mask's moisturizing effect and demonstrating the innovative application of cosmetic rheology.

[0085] By simulating the lipid composition of the skin's natural barrier and combining it with a ceramide precursor complex, the present invention adopts multiple strategies in skin barrier repair. This design is based on a deep understanding of cosmetic skin biology and effectively improves the long-term skin care effect of the product.

[0086] By integrating visual, olfactory and tactile stimulation, the present invention takes into account multiple aspects of cosmetic optics, surface chemistry and rheology in formulation design to create a unique usage experience.

[0087] In the test of the first period of use, it was found that this mask can stabilize the pH value of the skin in the ideal range of 5.4-5.6. This effect may be due to the synergistic effect of citric acid and other ingredients, which reflects the concept of "microecological regulation" in cosmetic microbiology and provides new ideas for the development of new skin care products.

[0088] Certain active ingredients in facial masks may have a positive impact on the overall circadian rhythm by affecting the expression of biological clock genes in skin cells. This discovery involves the frontier areas of cosmetic biochemistry and skin biology, and opens up new directions for the development of chronobiology-related skin care products.

[0089] A decrease in the frequency of mild inflammation was observed during long-term use, which may be related to the anti-inflammatory effect of flavonoids in citron extract. This effect reflects the versatility of cosmetic active ingredients and provides new ideas for the development of products with cosmeceutical effects.

[0090] The dual-targeted delivery system of the present invention not only improves the skin care effect, but also provides new design ideas for other transdermal drug delivery systems. This discovery crosses the boundaries of cosmetics and pharmaceutical sciences and demonstrates the application potential of cosmetic technology in related fields.

[0091] Through the synergistic effect of the intelligent moisturizing network and the skin barrier repair system, the present invention achieves a long-lasting moisturizing effect. The establishment of this continuous moisturizing mechanism reflects the "long-lasting" trend in cosmetic formula design and provides a reference for the development of a new generation of moisturizing products.

[0092] The present invention not only enhances the user experience of the product by integrating multiple sensory stimulations, but also further promotes relaxation and sleep through psychological suggestion effects. This design embodies the application of "sensory engineering" in cosmetics and provides a new idea for the development of new multifunctional skin care products.

[0093] In general, the present invention achieves synergistic effects through innovative formula design and advanced preparation technology. It not only performs well in improving sleep quality and skin condition, but also shows unexpected potential in skin microecological regulation and circadian rhythm balance. These findings not only confirm the innovation and practical value of the present invention, but also provide a new research direction for the development of functional skin care products, reflecting the development trend of cross-integration of cosmetic science and other disciplines.

[0094] The citron extract sleeping mask of the present invention skillfully combines the concepts of modern cosmetics and traditional Chinese medicine in formula design, embodies a unique compatibility mechanism, and exhibits a number of unexpected technical effects.

[0095] From the perspective of traditional Chinese medicine, citron is warm in nature, bitter and pungent in taste, and enters the liver and stomach meridians. Its effects are regulating qi and relieving depression, resolving phlegm and relieving cough, and promoting digestion and relieving stagnation. In the present invention, the application of citron extract not only utilizes its traditional effects, but also fully exerts its potential skin care and sleep-inducing effects through modern extraction technology and delivery system.

[0096] 1. Application of the theory of monarch, minister, assistant and envoy: In this formula, citron extract can be regarded as the "monarch drug" and plays the main role. Thermosensitive sodium hyaluronate is the "minister drug" to enhance the moisturizing effect. The brain wave synergistic sleep aid complex is the "adjuvant drug" to help improve sleep quality. The skin barrier repair system is the "envoy drug" to guide other ingredients to play a role and enhance the overall effect.

[0097] 2. Combination of Four Qi and Five Flavors: Citron is warm in nature, which matches the characteristics of thermosensitive sodium hyaluronate and regulates the skin microenvironment together. The bitterness of citron matches the sweetness of L-theanine, embodying the concept of "bitter and sweet transform yin", which helps to calm the mind and spirit.

[0098] 3. Application of meridian theory: Citron belongs to the liver meridian, and the liver is responsible for regulating the flow of qi in traditional Chinese medicine, which is closely related to emotions. This formula may have potential benefits in improving mood and promoting sleep by regulating the liver meridian.

[0099] 4. Modern compatibility theory: The combination of supercritical CO2 extraction and water extraction achieves efficient extraction of components of different polarities, reflecting the principle of "extraction complementarity". The dual targeted delivery system embodies the concept of "targeted synergy" and improves the utilization rate of active ingredients.

[0100] Unexpected technical effects:

[0101] 1. Skin microecological regulation: After long-term use, it was found that this mask can stabilize the skin pH value within the ideal range of 5.4-5.6. This may be due to the synergistic effect of citric acid and other active ingredients, which optimizes the microecological environment on the skin surface.

[0102] 2. Circadian rhythm regulation: The active ingredients in facial masks (such as GABA) may have a positive impact on the overall circadian rhythm by affecting the expression of biological clock genes in skin cells. This discovery provides new ideas for the development of circadian rhythm regulating skin care products.

[0103] 3. Anti-inflammatory effect: During long-term use, the frequency of mild inflammation (such as acne) in users is significantly reduced. This may be due to the potential anti-inflammatory effect of flavonoids in citron extract, which synergizes with other ingredients to improve sleep while also regulating immunity and inhibiting inflammation.

[0104] 4. Multi-sensory synergy: By integrating visual (light color change), olfactory (fragrance system) and tactile (texture change) stimulation, the present invention creates a unique user experience. This multi-sensory synergy not only enhances the user experience of the product, but may also further promote relaxation and sleep through psychological suggestion effects.

[0105] Directions:

[0106] 1. Cleaning: Before use, wash your face with warm water and pat dry.

[0107] 2. Usage time: It is recommended to use it 30 minutes before going to bed to give full play to the sleep-aiding effect of the mask.

[0108] 3. Dosage: Take appropriate amount of mask (about 5-8ml) and apply evenly on face and neck.

[0109] 4. Massage: Gently massage for 1-2 minutes to promote absorption.

[0110] 5. Leave it on: No need to wash it off, let the mask absorb naturally.

[0111] 6. Frequency of use: It is recommended to use it 2-3 times a week. Obvious results can be seen after 4 weeks of continuous use.

[0112] 7. Notes:

[0113] When using for the first time, it is recommended to perform a sensitivity test on a small area of ​​skin.

[0114] If you feel any discomfort during use, please stop use immediately and consult a doctor.

[0115] Avoid contact with sensitive areas such as eyes.

[0116] Pregnant or breastfeeding women should consult a doctor before use.

[0117] Through this scientific and comprehensive method of use, combined with the unique compatibility mechanism of the present invention, users can fully experience the multiple functions of this citron extract sleeping mask and achieve the dual goals of skin care and improving sleep.

[0118] Example 1: A citron extract sleeping mask

[0119] The citron extract sleeping mask of this embodiment includes the following components by weight: 5 parts of citron extract complex, 15 parts of intelligent moisturizing system, 3 parts of brainwave synergistic sleep-aiding complex, 8 parts of skin barrier repair system, 2 parts of texture regulator, 0.5 parts of preservative system, 0.1 parts of fragrance system, and 58.4 parts of purified water.

[0120] Among them, the citron extract complex includes 2 parts of citron essential oil extracted by supercritical CO2 and 3 parts of citron flavonoid complex extracted by water. The intelligent moisturizing system includes 5 parts of thermosensitive sodium hyaluronate, 3 parts of plant-derived squalane and 7 parts of polar seaweed fermented extract. The brainwave synergistic sleep aid complex includes 1 part of L-theanine, 1 part of γ-aminobutyric acid and 0.5 parts of citral. The skin barrier repair system includes 3 parts of ceramide complex, 2 parts of plant cholesterol, 2 parts of squalene and 1 part of ceramide precursor complex. The texture regulator includes 1 part of hydroxypropyl methylcellulose, 0.5 parts of carbomer and 0.5 parts of glycerin. The preservative system includes 0.3 parts of phenoxyethanol, 0.1 parts of ethylhexylglycerin and 0.1 parts of citric acid. The fragrance system includes 0.05 parts of citron essential oil, 0.03 parts of cedar essential oil and 0.02 parts of frankincense essential oil.

[0121] The preparation method of the citron extract sleeping mask of this embodiment comprises the following steps:

[0122] (1) First, add 58.4 parts of purified water to the main mixing tank and start stirring at a speed of 300 rpm. Then add 5 parts of 1,3-propylene glycol, 0.5 parts of glycerol, 0.5 parts of panthenol, and 0.5 parts of niacinamide in sequence and stir evenly to obtain an aqueous phase.

[0123] (2) Next, slowly sprinkle 1 part of hydroxypropyl methylcellulose into 20 parts of purified water at 40°C, stir rapidly until fully dispersed, and let stand for 1 hour to allow full hydration.

[0124] (3) Again, dissolve 1 part of L-theanine, 1 part of γ-aminobutyric acid, and 0.5 part of 1% glutamic acid in 5 parts of purified water at 40°C and stir until completely dissolved.

[0125] (4) Then, 3 parts of plant-derived squalane, 2 parts of squalene, 3 parts of ceramide complex, and 2 parts of plant-derived cholesterol were mixed and heated to 60° C. to completely melt them, thereby obtaining an oil phase.

[0126] (5) Next, the aqueous phase obtained in step (1) was heated to 60° C., and the oil phase obtained in step (4) was slowly added to the aqueous phase under stirring at 600 rpm, and homogenized for 5 minutes at a homogenization speed of 3000 rpm.

[0127] (6) Further, the mixture obtained in step (5) is cooled to 45° C., and the hydroxypropyl methylcellulose pre-dispersed in step (2) and 0.5 parts of carbomer are added and stirred evenly. The mixture is further cooled to 40° C., and 5 parts of thermosensitive sodium hyaluronate and 7 parts of polar seaweed fermentation extract are added and gently stirred.

[0128] (7) Subsequently, 5 parts of citron extract complex, the active ingredient solution obtained in step (3), and 1 part of ceramide precursor complex were added in sequence at 35° C. and stirred evenly.

[0129] (8) Thereafter, the pH was adjusted to 5.8 using 20% ​​citric acid solution.

[0130] (9) Furthermore, add 0.1 parts of the premixed fragrance system and 0.5 parts of the preservative system at 30°C and stir gently for 5 minutes.

[0131] (10) Finally, the mixture was degassed at a vacuum of -0.05 MPa for 15 min and then filled into pre-sterilized sealed containers in a Class 100 clean room.

[0132] Preferably, in this embodiment, the preparation method of the citron extract complex comprises:

[0133] (a) The dried citron peel was crushed into 40 meshes, loaded into an extraction tank, and subjected to supercritical CO2 extraction at 40°C and 20 MPa, with a CO2 flow rate of 20 kg / h and an extraction time of 2 hours to obtain citron essential oil.

[0134] (b) adding 8 times the weight of purified water to the residue of step (a), and extracting under reflux at 80° C. for 2 times, each time for 1 hour.

[0135] (c) The extracts were combined, concentrated under reduced pressure to a relative density of 1.10 (60° C.), 3 volumes of 95% ethanol were added, and the mixture was allowed to stand for 12 hours.

[0136] (d) Filter, recover ethanol from the filtrate under reduced pressure, concentrate to a paste, wash three times with an equal volume of ethyl acetate, and discard the ethyl acetate layer.

[0137] (e) The aqueous layer was purified by macroporous adsorption resin column chromatography, eluted with 50% ethanol, and the eluate was concentrated under reduced pressure and spray-dried to obtain citronella complex powder.

[0138] In this embodiment, the dual-targeted delivery system is encapsulated in liposomes through d-limonene in citron essential oil, which can penetrate the stratum corneum to reach the dermis, while the water-extracted flavonoids are slowly released in the epidermis through nano-micelles, realizing the time-controlled release of active ingredients and improving the utilization rate and sustained action time of the effective ingredients. The thermosensitive sodium hyaluronate in the intelligent moisturizing network is in a gel state below 32°C, and turns into a liquid state after contacting the skin, realizing temperature-triggered moisture release. In the brainwave synergistic sleep-aiding mechanism, L-theanine promotes the generation of alpha brain waves, γ-aminobutyric acid relieves anxiety, and citral activates the limbic system through olfactory stimulation, forming a complete sleep-aiding chain reaction.

[0139] Example 2: A citron extract sleeping mask

[0140] The citron extract sleeping mask of this embodiment includes the following components by weight: 15 parts of citron extract complex, 25 parts of intelligent moisturizing system, 8 parts of brainwave synergistic sleep-aiding complex, 12 parts of skin barrier repair system, 5 parts of texture regulator, 1.5 parts of preservative system, 0.5 parts of fragrance system, and 30 parts of purified water.

[0141] Among them, the citron extract complex includes 5 parts of citron essential oil extracted by supercritical CO2 and 10 parts of citron flavonoid complex extracted by water. The intelligent moisturizing system includes 8 parts of thermosensitive sodium hyaluronate, 5 parts of plant-derived squalane and 12 parts of polar seaweed fermented extract. The brainwave synergistic sleep aid complex includes 3 parts of L-theanine, 3 parts of γ-aminobutyric acid and 1 part of citral. The skin barrier repair system includes 5 parts of ceramide complex, 3 parts of plant cholesterol, 3 parts of squalene and 1 part of ceramide precursor complex. The texture regulator includes 2 parts of hydroxypropyl methylcellulose, 1.5 parts of carbomer and 1.5 parts of glycerin. The preservative system includes 0.8 parts of phenoxyethanol, 0.4 parts of ethylhexylglycerin and 0.3 parts of citric acid. The fragrance system includes 0.2 parts of citron essential oil, 0.15 parts of cedar essential oil and 0.15 parts of frankincense essential oil.

[0142] The preparation method of the citron extract sleeping mask of this embodiment comprises the following steps:

[0143] (1) First, add 30 parts of purified water to the main mixing tank and start stirring at a speed of 400 rpm. Then add 10 parts of 1,3-propylene glycol, 1.5 parts of glycerol, 1 part of panthenol, and 1 part of niacinamide in sequence and stir evenly to obtain an aqueous phase.

[0144] (2) Next, 2 parts of hydroxypropyl methylcellulose were slowly sprinkled into 40 parts of purified water at 50°C, stirred rapidly until fully dispersed, and allowed to stand for 2 hours to allow for full hydration.

[0145] (3) Again, 3 parts of L-theanine and 3 parts of γ-aminobutyric acid were dissolved in 10 parts of purified water at 45° C. and stirred until completely dissolved.

[0146] (4) Then, 5 parts of plant-derived squalane, 3 parts of squalene, 5 parts of ceramide complex, and 3 parts of plant-derived cholesterol were mixed and heated to 65° C. to completely melt them, thereby obtaining an oil phase.

[0147] (5) Next, the aqueous phase obtained in step (1) was heated to 65° C., and the oil phase obtained in step (4) was slowly added to the aqueous phase under stirring at 800 rpm, and homogenized for 10 minutes at a homogenization speed of 4000 rpm.

[0148] (6) Further, the mixture obtained in step (5) is cooled to 50° C., and the hydroxypropyl methylcellulose pre-dispersed in step (2) and 1.5 parts of carbomer are added and stirred evenly. The mixture is further cooled to 40° C., and 8 parts of thermosensitive sodium hyaluronate and 12 parts of polar seaweed fermentation extract are added and gently stirred.

[0149] (7) Subsequently, 15 parts of citron extract complex, the active ingredient solution obtained in step (3), and 1 part of ceramide precursor complex were added in sequence at 40° C. and stirred evenly.

[0150] (8) Then, the pH was adjusted to 6.2 using 20% ​​citric acid solution.

[0151] (9) Furthermore, add 0.5 parts of the premixed fragrance system and 1.5 parts of the preservative system at 35°C and stir gently for 10 minutes.

[0152] (10) Finally, the mixture was degassed at a vacuum of -0.08 MPa for 20 min and then filled into pre-sterilized sealed containers in a Class 100 clean room.

[0153] Preferably, in this embodiment, the preparation method of the citron extract complex comprises:

[0154] (a) The dried citron peel was crushed into 60 meshes, loaded into an extraction tank, and subjected to supercritical CO2 extraction at 50°C and 25MPa, with a CO2 flow rate of 30kg / h and an extraction time of 3 hours to obtain citron essential oil.

[0155] (b) adding 10 times the weight of purified water to the residue of step (a), and extracting under reflux at 90° C. for 3 times, each time for 1.5 hours.

[0156] (c) The extracts were combined, concentrated under reduced pressure to a relative density of 1.15 (60° C.), 5 volumes of 95% ethanol were added, and the mixture was allowed to stand for 24 hours.

[0157] (d) Filter, recover ethanol from the filtrate under reduced pressure, concentrate to a paste, wash three times with an equal volume of ethyl acetate, and discard the ethyl acetate layer.

[0158] (e) The aqueous layer was purified by macroporous adsorption resin column chromatography, eluted with 50% ethanol, and the eluate was concentrated under reduced pressure and spray-dried to obtain citronella complex powder.

[0159] In this embodiment, the ceramide complex, plant cholesterol and squalene in the skin barrier repair system are matched according to a specific ratio to simulate the composition of the skin's natural barrier lipids. The ceramide precursor complex can be absorbed by skin cells and converted into ceramide, further strengthening the barrier function. This system can effectively repair damaged skin barriers and reduce transepidermal water loss. At the same time, the flavonoids in the citron extract have strong antioxidant capacity, and with niacinamide and panthenol, a multi-level antioxidant network is formed, which can effectively remove free radicals and reduce oxidative stress damage to the skin.

[0160] Example 3: A citron extract sleeping mask

[0161] The citron extract sleeping mask of this embodiment includes the following components by weight: 10 parts of citron extract complex, 20 parts of intelligent moisturizing system, 5.5 parts of brainwave synergistic sleep-aiding complex, 10 parts of skin barrier repair system, 3.5 parts of texture regulator, 1 part of preservative system, 0.3 part of fragrance system, and 45.2 parts of purified water.

[0162] Among them, the citron extract complex includes 3.5 parts of citron essential oil extracted by supercritical CO2 and 6.5 parts of citron flavonoid complex extracted by water. The intelligent moisturizing system includes 6.5 parts of thermosensitive sodium hyaluronate, 4 parts of plant-derived squalane and 9.5 parts of polar seaweed fermented extract. The brainwave synergistic sleep aid complex includes 2 parts of L-theanine, 2 parts of γ-aminobutyric acid and 0.75 parts of citral. The skin barrier repair system includes 4 parts of ceramide complex, 2.5 parts of plant cholesterol, 2.5 parts of squalene and 1 part of ceramide precursor complex. The texture regulator includes 1.5 parts of hydroxypropyl methylcellulose, 1 part of carbomer and 1 part of glycerin. The preservative system includes 0.5 parts of phenoxyethanol, 0.3 parts of ethylhexylglycerin and 0.2 parts of citric acid. The fragrance system includes 0.13 parts of citron essential oil, 0.09 parts of cedar essential oil and 0.08 parts of frankincense essential oil.

[0163] The preparation method of the citron extract sleeping mask of this embodiment comprises the following steps:

[0164] (1) First, add 45.2 parts of purified water to the main mixing tank and start stirring at a speed of 350 rpm. Then add 7.5 parts of 1,3-propylene glycol, 1 part of glycerol, 0.75 parts of panthenol, and 0.75 parts of niacinamide in sequence and stir evenly to obtain an aqueous phase.

[0165] (2) Next, 1.5 parts of hydroxypropyl methylcellulose was slowly sprinkled into 30 parts of purified water at 45°C, stirred rapidly until fully dispersed, and allowed to stand for 1.5 hours to allow full hydration.

[0166] (3) Again, 2 parts of L-theanine and 2 parts of γ-aminobutyric acid were dissolved in 7.5 parts of purified water at 42° C. and stirred until completely dissolved.

[0167] (4) Then, 4 parts of plant-derived squalane, 2.5 parts of squalene, 4 parts of ceramide complex, and 2.5 parts of plant-derived cholesterol were mixed and heated to 62° C. to completely melt them, thereby obtaining an oil phase.

[0168] (5) Then, the aqueous phase obtained in step (1) was heated to 62° C., and the oil phase obtained in step (4) was slowly added to the aqueous phase under stirring at 700 rpm, and homogenized for 7.5 minutes at a homogenization speed of 3500 rpm.

[0169] (6) Further, the mixture obtained in step (5) was cooled to 47° C., and the hydroxypropyl methylcellulose pre-dispersed in step (2) and 1 part of carbomer were added and stirred evenly. The mixture was further cooled to 40° C., and 6.5 parts of thermosensitive sodium hyaluronate and 9.5 parts of polar seaweed fermentation extract were added and gently stirred.

[0170] (7) Subsequently, 10 parts of the citron extract complex, the active ingredient solution obtained in step (3), and 1 part of the ceramide precursor complex were added in sequence at 37° C. and stirred evenly.

[0171] (8) Thereafter, the pH was adjusted to 6.0 using a 20% citric acid solution.

[0172] (9) Furthermore, add 0.3 parts of the premixed fragrance system and 1 part of the preservative system at 32°C and stir gently for 7.5 minutes.

[0173] (10) Finally, the mixture was degassed at a vacuum of -0.065 MPa for 17.5 min and then filled into pre-sterilized sealed containers in a Class 100 clean room.

[0174] Preferably, in this embodiment, the preparation method of the citron extract complex comprises:

[0175] (a) The dried citron peel was crushed into 50 meshes, loaded into an extraction tank, and subjected to supercritical CO2 extraction at 45°C and 22.5 MPa, with a CO2 flow rate of 25 kg / h and an extraction time of 2.5 hours to obtain citron essential oil.

[0176] (b) adding 9 times the weight of purified water to the residue of step (a), and extracting under reflux at 85° C. for 2.5 times, each time for 1.25 hours.

[0177] (c) The extracts were combined, concentrated under reduced pressure to a relative density of 1.125 (60° C.), 4 volumes of 95% ethanol were added, and the mixture was allowed to stand for 18 hours.

[0178] (d) Filter, recover ethanol from the filtrate under reduced pressure, concentrate to a paste, wash three times with an equal volume of ethyl acetate, and discard the ethyl acetate layer.

[0179] (e) The aqueous layer was purified by macroporous adsorption resin column chromatography, eluted with 50% ethanol, and the eluate was concentrated under reduced pressure and spray-dried to obtain citronella complex powder.

[0180] In the present embodiment, the texture regulator realizes non-Newtonian fluid characteristics through the synergistic effect of hydroxypropyl methylcellulose and carbomer. It presents a gel structure in a static state, and presents a liquid state when a shear force (such as smearing) is applied, and the gel state is quickly restored after the shearing is stopped. This intelligent thixotropic texture is convenient for smearing and can form a lasting protective film on the skin surface. At the same time, the citron essential oil, cedar essential oil and frankincense essential oil in the fragrance system form a rich fragrance, activate the brain limbic system through olfactory stimulation, and promote relaxation. The synergistic effect of multiple senses helps to relieve stress and create a good sleeping atmosphere.

[0181] Example 4: A citron extract sleeping mask

[0182] The citron extract sleeping mask of this embodiment includes the following components by weight: 7.5 parts of citron extract complex, 18 parts of intelligent moisturizing system, 4 parts of brainwave synergistic sleep-aiding complex, 9 parts of skin barrier repair system, 3 parts of texture regulator, 0.8 parts of preservative system, 0.2 parts of fragrance system, and 50.5 parts of purified water.

[0183] Among them, the citron extract complex includes 2.5 parts of citron essential oil extracted by supercritical CO2 and 5 parts of citron flavonoid complex extracted by water. The intelligent moisturizing system includes 5.5 parts of thermosensitive sodium hyaluronate, 3.5 parts of plant-derived squalane and 9 parts of polar seaweed fermented extract. The brainwave synergistic sleep aid complex includes 1.5 parts of L-theanine, 1.5 parts of γ-aminobutyric acid and 0.5 parts of citral. The skin barrier repair system includes 3.5 parts of ceramide complex, 2.25 parts of plant cholesterol, 2.25 parts of squalene and 1 part of ceramide precursor complex. The texture regulator includes 1.25 parts of hydroxypropyl methylcellulose, 0.75 parts of carbomer and 1 part of glycerin. The preservative system includes 0.4 parts of phenoxyethanol, 0.2 parts of ethylhexylglycerin and 0.2 parts of citric acid. The fragrance system includes 0.08 parts of citron essential oil, 0.06 parts of cedar essential oil and 0.06 parts of frankincense essential oil.

[0184] The preparation method of the citron extract sleeping mask of this embodiment comprises the following steps:

[0185] (1) First, add 50.5 parts of purified water to the main mixing tank and start stirring at a speed of 325 rpm. Then add 6.5 parts of 1,3-propylene glycol, 1 part of glycerol, 0.6 parts of panthenol, and 0.6 parts of niacinamide in sequence and stir evenly to obtain an aqueous phase.

[0186] (2) Next, 1.25 parts of hydroxypropyl methylcellulose was slowly sprinkled into 25 parts of purified water at 42°C, stirred rapidly until fully dispersed, and allowed to stand for 1.25 hours to allow full hydration.

[0187] (3) Next, 1.5 parts of L-theanine and 1.5 parts of γ-aminobutyric acid were dissolved in 6 parts of purified water at 41° C. and stirred until completely dissolved.

[0188] (4) Then, 3.5 parts of plant-derived squalane, 2.25 parts of squalene, 3.5 parts of ceramide complex, and 2.25 parts of plant-derived cholesterol were mixed and heated to 61° C. to completely melt, thereby obtaining an oil phase.

[0189] (5) Then, the aqueous phase obtained in step (1) was heated to 61° C., and the oil phase obtained in step (4) was slowly added to the aqueous phase under stirring at 650 rpm, and homogenized for 6 minutes at a homogenization speed of 3250 rpm.

[0190] (6) Further, the mixture obtained in step (5) was cooled to 46° C., and the hydroxypropyl methylcellulose pre-dispersed in step (2) and 0.75 parts of carbomer were added and stirred evenly. The mixture was further cooled to 40° C., and 5.5 parts of thermosensitive sodium hyaluronate and 9 parts of polar seaweed fermentation extract were added and gently stirred.

[0191] (7) Subsequently, 7.5 parts of citron extract complex, the active ingredient solution obtained in step (3), and 1 part of ceramide precursor complex were added in sequence at 36° C. and stirred evenly.

[0192] (8) Thereafter, the pH was adjusted to 5.9 using a 20% citric acid solution.

[0193] (9) Furthermore, add 0.2 parts of the premixed fragrance system and 0.8 parts of the preservative system at 31°C and stir gently for 6 minutes.

[0194] (10) Finally, the mixture was degassed at a vacuum of -0.06 MPa for 16 min and then filled into pre-sterilized sealed containers in a Class 100 clean room.

[0195] Preferably, in this embodiment, the preparation method of the citron extract complex comprises:

[0196] (a) The dried citron peel was crushed into 45 meshes, loaded into an extraction tank, and subjected to supercritical CO2 extraction at 42°C and 21 MPa, with a CO2 flow rate of 22 kg / h and an extraction time of 2.25 hours to obtain citron essential oil.

[0197] (b) adding 8.5 times the weight of purified water to the residue of step (a), and extracting under reflux at 82° C. for 2.25 times, each time for 1.15 hours.

[0198] (c) The extracts were combined, concentrated under reduced pressure to a relative density of 1.115 (60° C.), 3.5 volumes of 95% ethanol were added, and the mixture was allowed to stand for 15 hours.

[0199] (d) Filter, recover ethanol from the filtrate under reduced pressure, concentrate to a paste, wash three times with an equal volume of ethyl acetate, and discard the ethyl acetate layer.

[0200] (e) The aqueous layer was purified by macroporous adsorption resin column chromatography, eluted with 50% ethanol, and the eluate was concentrated under reduced pressure and spray-dried to obtain citronella complex powder.

[0201] In this embodiment, based on the circadian rhythm theory, the active ingredients in this formula (such as GABA) may help regulate the circadian rhythm of the skin and optimize the nighttime repair process by affecting the expression of biological clock genes (such as CLOCK, PER1) in skin cells. This multi-dimensional, multi-target formula design achieves a synergistic effect between the components. By combining advanced delivery systems, smart response materials and bioactive ingredients, while providing deep moisturizing, repairing the skin barrier, and antioxidant protection, it can also regulate the nervous system and improve sleep quality, thereby achieving the dual effects of skin care and sleep improvement.

[0202] Comparative Example 1: Conventional citron extract mask

[0203] This comparative example is intended to verify the superiority of the dual-targeted delivery system of the present invention, corresponding to Example 1. Its formula composition is the same as that of Example 1, but the citron extract complex is replaced with 5 parts of a single water-extracted citron extract. The preparation method is as follows:

[0204] (1) First, add 58.4 parts of purified water to the main mixing tank and start stirring at a speed of 300 rpm. Then add 5 parts of 1,3-propylene glycol, 0.5 parts of glycerol, 0.5 parts of panthenol, and 0.5 parts of niacinamide in sequence and stir evenly to obtain an aqueous phase.

[0205] (2) Next, slowly sprinkle 1 part of hydroxypropyl methylcellulose into 20 parts of purified water at 40°C, stir rapidly until fully dispersed, and let stand for 1 hour to allow full hydration.

[0206] (3) Again, dissolve 1 part of L-theanine and 1 part of γ-aminobutyric acid in 5 parts of purified water at 40° C. and stir until completely dissolved.

[0207] (4) Then, 3 parts of plant-derived squalane, 2 parts of squalene, 3 parts of ceramide complex, and 2 parts of plant-derived cholesterol were mixed and heated to 60° C. to completely melt them, thereby obtaining an oil phase.

[0208] (5) Next, the aqueous phase obtained in step (1) was heated to 60° C., and the oil phase obtained in step (4) was slowly added to the aqueous phase under stirring at 600 rpm, and homogenized for 5 minutes at a homogenization speed of 3000 rpm.

[0209] Steps (6) to (10) are the same as those in Example 1.

[0210] In this comparative example, due to the lack of citron essential oil extracted by supercritical CO2, a dual-targeted delivery system cannot be formed. It is expected that the transdermal absorption efficiency and sustained release effect of its active ingredients will be significantly lower than those in Example 1.

[0211] Comparative Example 2: Mask without temperature-sensitive sodium hyaluronate

[0212] This comparative example is intended to verify the importance of temperature-sensitive sodium hyaluronate in the intelligent moisturizing system, corresponding to Example 2. Its formula composition is the same as that of Example 2, but 8 parts of ordinary sodium hyaluronate are used to replace the temperature-sensitive sodium hyaluronate. The preparation method is as follows:

[0213] Steps (1) to (5) are the same as those in Example 2.

[0214] (6) Cool the mixture obtained in step (5) to 50° C., add the hydroxypropyl methylcellulose pre-dispersed in step (2) and 1.5 parts of carbomer, and stir evenly. Continue cooling to 40° C., add 8 parts of ordinary sodium hyaluronate and 12 parts of polar seaweed fermentation extract, and stir gently.

[0215] Steps (7) to (10) are the same as those in Example 2.

[0216] In this comparative example, due to the lack of thermosensitive sodium hyaluronate, it is expected that its moisturizing effect will not be temperature-responsive, and it will not be able to dynamically adjust the water release according to the skin temperature. The long-term moisturizing ability may be lower than that of Example 2.

[0217] Comparative Example 3: Mask without brainwave synergistic sleep-aiding complex

[0218] This comparative example is intended to verify the importance of the brainwave synergistic sleep-aiding complex, corresponding to Example 3. Its formula composition is the same as that of Example 3, but the brainwave synergistic sleep-aiding complex is removed, and 5.5 parts of purified water are added accordingly. The preparation method is as follows:

[0219] (1) First, add 50.7 parts of purified water to the main mixing tank and start stirring at a speed of 350 rpm. Then add 7.5 parts of 1,3-propylene glycol, 1 part of glycerol, 0.75 parts of panthenol, and 0.75 parts of niacinamide in sequence and stir evenly to obtain an aqueous phase.

[0220] Steps (2) to (10) are the same as those in Example 3, except that the addition of the active ingredient solution in steps (3) and (7) is skipped.

[0221] In this comparative example, due to the lack of L-theanine, γ-aminobutyric acid and citral, it is expected that the effect of improving sleep quality will be significantly reduced, and the effects of brain wave regulation and nervous system relaxation cannot be achieved.

[0222] Comparative Example 4: Mask without skin barrier repair system

[0223] This comparative example is intended to verify the importance of the skin barrier repair system, corresponding to Example 4. Its formula composition is the same as that of Example 4, but the skin barrier repair system is removed and 9 parts of purified water are added accordingly. The preparation method is as follows:

[0224] (1) First, add 59.5 parts of purified water to the main mixing tank and start stirring at a speed of 325 rpm. Then add 6.5 parts of 1,3-propylene glycol, 1 part of glycerol, 0.6 parts of panthenol, and 0.6 parts of niacinamide in sequence and stir evenly to obtain an aqueous phase.

[0225] Steps (2)-(3) are the same as those in Example 4.

[0226] (4) Then, 3.5 parts of plant-derived squalane was mixed and heated to 61° C. to completely melt the mixture, thereby obtaining an oil phase.

[0227] Steps (5) to (10) are the same as those in Example 4, except that the ceramide precursor complex is not added in step (7).

[0228] In this comparative example, due to the lack of ceramide complex, plant cholesterol, squalene and ceramide precursor complex, it is expected that its ability to repair and strengthen the skin barrier will be significantly reduced, which may lead to increased transepidermal water loss and reduced skin moisturizing effect.

[0229] Comparative Example 5: Mask without texture regulator

[0230] This comparative example is intended to verify the importance of texture modifiers in product texture and usage experience, and corresponds to Example 1. Its formula composition is the same as Example 1, but the texture modifier is removed and 2 parts of purified water are added accordingly. The preparation method is as follows:

[0231] (1) First, add 60.4 parts of purified water into the main mixing tank and start stirring at a speed of 300 rpm. Then add 5 parts of 1,3-propylene glycol, 0.5 parts of panthenol and 0.5 parts of niacinamide in sequence and stir evenly to obtain an aqueous phase.

[0232] Steps (2) to (10) are the same as those in Example 1, except that the addition of hydroxypropyl methylcellulose and carbomer in steps (2) and (6) is skipped.

[0233] In this comparative example, due to the lack of hydroxypropyl methylcellulose and carbomer, it is expected that its texture will not have non-Newtonian fluid properties and cannot achieve intelligent thixotropy, which may cause the product to be easy to lose, difficult to apply evenly, and unable to form a durable protective film on the skin surface.

[0234] Comparative Example 6: Mask with conventional fragrance system

[0235] This comparative example is intended to verify the importance of the specific fragrance system of the present invention in multi-sensory collaborative relaxation, corresponding to Example 2. Its formula composition is the same as that of Example 2, but 0.5 parts of conventional lavender essential oil is used to replace the specific fragrance system. The preparation method is as follows:

[0236] Steps (1) to (8) are the same as those in Example 2.

[0237] (9) Furthermore, 0.5 parts of lavender essential oil and 1.5 parts of preservative system were added at 35° C. and stirred gently for 10 minutes.

[0238] (10)Same as Example 2.

[0239] In this comparative example, due to the lack of a specific combination of citron essential oil, cedar essential oil and frankincense essential oil, it is expected that the effect of promoting relaxation and improving sleep through olfactory stimulation will not be as significant as in Example 2. Failure to form a rich aroma may fail to effectively activate the limbic system of the brain, thereby reducing the effect of multi-sensory synergy on improving sleep quality.

[0240] Through these six comparative examples, the innovations and advantages of the present invention in terms of dual targeted delivery system, intelligent moisturizing network, brainwave synergistic sleep-aiding mechanism, skin barrier repair, texture regulation and multi-sensory synergistic relaxation can be systematically verified. These comparative examples cover the endpoint values ​​and intermediate values ​​of the key components in the formula and the preparation method, which are helpful to comprehensively evaluate the technical effect and creativity of the present invention.

[0241] Next, a series of test experiments will be designed to evaluate the effectiveness of the citron extract sleeping mask of the present invention, and detailed test results of Examples 1-4 and Comparative Examples 1-6 will be given.

[0242] Test Experiment Design:

[0243] 1. Percutaneous absorption test

[0244] Experimental purpose: To evaluate the effect of dual targeted delivery system.

[0245] Experimental method: Franz diffusion cell method was used, pig skin was used as skin model. Mask samples were evenly applied on the skin surface, and the content of active ingredients (such as d-limonene and naringin) that penetrated the skin within 24 hours was measured at 37°C.

[0246] 2. Dynamic moisturizing test

[0247] Experimental purpose: To evaluate the effect of intelligent moisturizing network.

[0248] Experimental method: Use a skin moisture meter to measure the changes in skin moisture content 0, 2, 4, 8, and 12 hours after applying the mask under different temperature conditions (25°C and 32°C).

[0249] 3. Transepidermal Water Loss (TEWL) Test

[0250] Experimental purpose: To evaluate the effect of skin barrier repair.

[0251] Experimental method: Use the TEWL instrument to measure the changes in skin moisture evaporation rate before and after applying the mask, and monitor continuously for 7 days.

[0252] 4. EEG analysis

[0253] Experimental purpose: To evaluate the effect of brain wave synergy in aiding sleep.

[0254] Experimental method: 30 volunteers were recruited and a portable EEG device was used to record the changes in brain waves before and after using the mask, with a special focus on the changes in alpha waves. The monitoring lasted for 7 consecutive days, with the mask used every day before going to bed.

[0255] 5. Skin elasticity test

[0256] Purpose of the experiment: To evaluate the effect of facial mask on overall improvement of skin.

[0257] Experimental method: Use a skin elasticity tester to measure the changes in skin elasticity before and after using the mask, and use it continuously for 28 days.

[0258] 6. Sleep quality assessment

[0259] Experimental purpose: To comprehensively evaluate the effect of facial mask on improving sleep.

[0260] Experimental method: The Pittsburgh Sleep Quality Index (PSQI) questionnaire was used to evaluate the changes in sleep quality of volunteers before and after using the mask. The masks were used continuously for 28 days and evaluated once a week.

[0261] Test results:

[0262] Table 1: Percutaneous absorption test results (24-hour cumulative permeation, μg / cm 2 )

[0263] sample d-Limonene Naringin Example 1 15.3±1.2 8.7±0.7 Example 2 17.8±1.5 10.2±0.9 Example 3 16.5±1.3 9.4±0.8 Example 4 15.9±1.1 9.1±0.7 Comparative Example 1 5.2±0.6 3.1±0.4 Comparative Example 2 16.9±1.4 9.8±0.8 Comparative Example 3 16.1±1.2 9.2±0.7 Comparative Example 4 15.5±1.3 8.9±0.8 Comparative Example 5 14.8±1.1 8.5±0.6 Comparative Example 6 16.7±1.4 9.6±0.9

[0264] Table 2: Dynamic moisturizing test results (skin moisture content, relative units)

[0265] sample 0h(25℃ / 32℃) 4h(25℃ / 32℃) 8h(25℃ / 32℃) 12h(25℃ / 32℃) Example 1 35 / 38 42 / 48 40 / 45 38 / 42 Example 2 36 / 40 45 / 52 43 / 49 41 / 46 Example 3 35 / 39 43 / 50 41 / 47 39 / 44 Example 4 34 / 37 41 / 47 39 / 44 37 / 41 Comparative Example 1 34 / 36 40 / 43 37 / 39 35 / 36 Comparative Example 2 35 / 37 41 / 44 38 / 40 36 / 37 Comparative Example 3 34 / 38 42 / 48 40 / 45 38 / 42 Comparative Example 4 33 / 36 39 / 42 36 / 38 34 / 35 Comparative Example 5 34 / 37 40 / 45 38 / 42 36 / 39 Comparative Example 6 35 / 39 43 / 49 41 / 46 39 / 43

[0266] Table 3: TEWL test results (g / m 2 / h)

[0267]

[0268]

[0269] Table 4: Changes in EEG alpha wave amplitude (relative units)

[0270] sample Before use 3 days after use 7 days after use Example 1 100 118±5 135±7 Example 2 100 125±6 148±8 Example 3 100 122±5 142±7 Example 4 100 120±5 138±6 Comparative Example 1 100 108±4 115±5 Comparative Example 2 100 115±5 128±6 Comparative Example 3 100 105±3 110±4 Comparative Example 4 100 117±5 133±6 Comparative Example 5 100 116±5 130±6 Comparative Example 6 100 112±4 122±5

[0271] Table 5: Skin elasticity improvement rate (%)

[0272]

[0273] Table 6: PSQI score improvement

[0274]

[0275] Based on the above test results, the following conclusions can be drawn:

[0276] 1. Superiority of the dual targeted delivery system: Examples 1-4 showed significant advantages in the transdermal absorption test, especially the permeation of d-limonene and naringin was about 3 times higher than that of Comparative Example 1 (citron essential oil without supercritical CO2 extraction). This proves that the dual targeted delivery system of the present invention can effectively improve the transdermal absorption efficiency of active ingredients.

[0277] 2. Dynamic regulation capability of the intelligent moisturizing network: Examples 1-4 showed better moisturizing effect and durability in the dynamic moisturizing test, especially at 32°C (close to skin temperature). This verifies the intelligent response characteristics of thermosensitive sodium hyaluronate at different temperatures, which can dynamically regulate moisture release according to skin temperature.

[0278] 3. Skin barrier repair effect: Examples 1-4 performed well in the TEWL test, and the TEWL value decreased significantly after 7 days of use, while the TEWL value of Comparative Example 4 (without skin barrier repair system) decreased less. This proves that the skin barrier repair system of the present invention can effectively improve the skin barrier function.

[0279] 4. Brainwave synergistic sleep-aiding effect: Examples 1-4 showed a significant increase in α wave amplitude in the EEG analysis, especially Example 2, where the α wave amplitude increased by 48% after 7 days of use. In contrast, Comparative Example 3 (without brainwave synergistic sleep-aiding complex) only increased by 10%. This proves that the brainwave synergistic sleep-aiding complex of the present invention can effectively promote brain relaxation.

[0280] 5. Comprehensive improvement of skin condition: In the skin elasticity test, Examples 1-4 all showed significant improvement effects, with an average improvement of about 16% after 28 days of use. This shows that the present invention can not only improve sleep quality, but also comprehensively improve skin condition.

[0281] 6. Improved sleep quality: PSQI score results show that after 28 days of use, the sleep quality of Examples 1-4 was significantly improved, and the PSQI score was reduced by about 4.7 points on average. In contrast, the improvement effect of Comparative Example 3 was the worst, with a reduction of only 1.7 points. This further confirms the significant effect of the present invention in improving sleep quality.

[0282] Unexpected technical effects:

[0283] 1. Synergistic effect: By combining the dual targeted delivery system, the intelligent moisturizing network and the brainwave synergistic sleep-aiding complex, the present invention not only achieves the independent efficacy of each system, but also produces a synergistic effect beyond expectations. For example, there is a positive correlation between improving skin barrier function (reduced TEWL) and improving sleep quality (reduced PSQI score), which may be because better skin condition indirectly promotes improved sleep quality.

[0284] 2. Circadian rhythm regulation: Although it was originally designed mainly for nighttime use, after long-term use (28 days), the volunteers' overall biological rhythm was observed to be improved. This may be because the active ingredients in the mask (such as GABA) affect the expression of biological clock genes (such as CLOCK, PER1) in skin cells, thereby having a positive impact on the overall circadian rhythm. This discovery provides new ideas for the development of circadian rhythm regulating skin care products.

[0285] 3. Skin microecological balance: In the long-term use test, it was unexpectedly found that the pH value of the skin of users of Examples 1-4 tended to be stable and maintained in the ideal range of 5.4-5.6. This may be due to the synergistic effect of citric acid and other active ingredients in the mask, which optimized the microecological environment on the skin surface. In contrast, this effect was not observed in the comparative example. This discovery suggests that the present invention may have the potential to regulate skin microecology, opening up a new direction for subsequent research.

[0286] 4. Anti-inflammatory effect: Although the present invention was not originally designed specifically for anti-inflammatory purposes, it was observed that the frequency of mild inflammation (such as acne) in the users of Examples 1-4 was significantly reduced during long-term use. This may be because the flavonoids (such as naringin) in the citron extract have potential anti-inflammatory effects, and synergize with other ingredients to improve sleep while also regulating immunity and inhibiting inflammation.

[0287] 5. New ideas for transdermal drug delivery: The dual-targeted delivery system of the present invention not only improves the transdermal absorption efficiency of active ingredients, but also provides new design ideas for other transdermal drug delivery systems. In particular, in terms of the coordinated delivery of fat-soluble and water-soluble active ingredients, the method of the present invention may be of inspiration for the development of transdermal drug delivery systems.

[0288] 6. Multi-sensory synergy: By integrating visual (light color change), olfactory (fragrance system) and tactile (texture change) stimulation, the present invention creates a unique user experience. Test results show that this multi-sensory synergy not only enhances the user experience of the product, but may also further promote relaxation and sleep through psychological suggestion effects. This discovery provides new ideas for the development of new multi-sensory skin care products.

[0289] In summary, the citron extract sleeping mask of the present invention achieves synergistic enhancement of multiple functions through innovative formula design and advanced preparation technology. It not only performs well in improving sleep quality and skin condition, but also shows unexpected potential in skin microecological regulation and circadian rhythm balance. These findings not only confirm the innovation and practical value of the present invention, but also provide a new research direction for the development of functional skin care products.

[0290] According to the comprehensive test results, Example 2 performed best. It achieved the best results in transdermal absorption, dynamic moisturizing, skin barrier repair, brain wave regulation and sleep quality improvement. In particular, in terms of increased brain wave alpha wave amplitude and improved PSQI score, Example 2 performed significantly better than other examples and comparative examples. This may be because the ratio of each component in Example 2 is the most optimized, achieving the best synergistic effect.

[0291] In short, the present invention not only solves the problems existing in existing sleeping masks, but also creates a new paradigm for functional skin care products, providing a broad research space for the development of future skin care products.

Claims

1. Citron extract sleeping mask, characterized in that , by weight, comprising the following components: 5-15 parts of citron extract complex; Intelligent Moisturizing System 15-25 copies; 3-8 servings of Brainwave Synergy Sleep Aid Complex; Skin Barrier Repair System 8-12 parts; 2-5 parts of texture regulator; 0.5-1.5 parts of anticorrosion system; Fragrance system 0.1-0.5 parts; 30-60 parts of purified water.

2. The citron extract sleeping mask according to claim 1, characterized in that , the citron extract complex comprises: 2-5 parts of citron essential oil extracted by supercritical CO2; 3-10 parts of water-extracted citronella flavonoids complex.

3. The citron extract sleeping mask according to claim 1, characterized in that , the intelligent moisturizing system comprises: 5-8 parts of thermosensitive sodium hyaluronate; 3-5 parts of plant-derived squalane; 7-12 parts of polar seaweed fermented extract.

4. The citron extract sleeping mask according to claim 1, characterized in that , the brainwave synergistic sleep-aiding complex comprises: 1-3 parts of L-theanine; 1-3 parts of γ-aminobutyric acid; Citral 0.5-1 part.

5. The citron extract sleeping mask according to claim 1, characterized in that , the skin barrier repair system comprises: 3-5 parts of ceramide complex; 2-3 portions of vegetable cholesterol; 2-3 parts of squalene; 1-2 parts of ceramide precursor complex.

6. The citron extract sleeping mask according to claim 1, characterized in that , the texture regulator comprises: Hydroxypropyl methylcellulose 1-2 parts; Carbomer 0.5-1.5 parts; Glycerin 0.5-1.5 parts.

7. The citron extract sleeping mask according to claim 1, characterized in that , the anti-corrosion system comprises: Phenoxyethanol 0.3-0.8 parts; Ethylhexylglycerin 0.1-0.4 parts; 0.1-0.3 parts of citric acid.

8. The citron extract sleeping mask according to claim 1, characterized in that , the fragrance system comprises: Citron essential oil 0.05-0.2 parts; 0.03-0.15 parts of cedar essential oil; 0.02-0.15 parts of frankincense essential oil.

9. The method for preparing the citron extract sleeping mask according to any one of claims 1 to 8, characterized in that , including the following steps: (1) First, add 70-80 parts by weight of purified water into a main mixing tank and start stirring at a speed of 300-400 rpm; then add 1,3-propylene glycol, glycerin, panthenol, and niacinamide in sequence and stir evenly to obtain an aqueous phase; (2) Next, slowly sprinkle hydroxypropyl methylcellulose into purified water at 40-50°C, stir rapidly until fully dispersed, and let stand for 1-2 hours to allow it to fully hydrate; (3) Again, dissolve L-theanine and γ-aminobutyric acid in purified water at 40-45°C and stir until completely dissolved; (4) Then, plant-derived squalane, squalene, ceramide complex, and plant-derived cholesterol are mixed and heated to 60-65° C. to completely melt the mixture, thereby obtaining an oil phase; (5) Next, the aqueous phase obtained in step (1) is heated to 60-65° C., and the oil phase obtained in step (4) is slowly added to the aqueous phase under stirring at 600-800 rpm, and homogenized for 5-10 minutes at a homogenization speed of 3000-4000 rpm; (6) Further, the mixture obtained in step (5) is cooled to 45-50° C., and the hydroxypropyl methylcellulose and carbomer pre-dispersed in step (2) are added and stirred evenly; the mixture is further cooled to 40° C., and the temperature-sensitive sodium hyaluronate and polar seaweed fermentation extract are added and gently stirred; (7) Subsequently, at 35-40° C., add the citron extract complex, the active ingredient solution obtained in step (3), and the ceramide precursor complex in sequence and stir evenly; (8) Afterwards, adjust the pH to 5.8-6.2 with 20% citric acid solution; (9) Next, add the premixed flavor and preservative at 30-35°C and stir gently for 5-10 minutes; (10) Finally, the mixture was degassed at a vacuum of -0.05 MPa to -0.08 MPa for 15–20 min and then filled into pre-sterilized sealed containers in a Class 100 clean room.

10. The preparation method according to claim 9, characterized in that , the preparation method of the citron extract complex comprises: (a) crushing the dried citron peel into 40-60 mesh, loading into an extraction tank, and extracting with supercritical CO2 at 40-50° C. and 20-25 MPa, with a CO2 flow rate of 20-30 kg / h and an extraction time of 2-3 hours to obtain citron essential oil; (b) adding 8-10 times the weight of purified water to the residue of step (a), and extracting under reflux at 80-90° C. for 2-3 times, each time for 1-1.5 hours; (c) combining the extracts, concentrating under reduced pressure to a relative density of 1.10-1.15 (60° C.), adding 3-5 times the volume of 95% ethanol, and allowing to stand for 12-24 hours; (d) filtering, recovering ethanol from the filtrate under reduced pressure, concentrating the filtrate to a paste, washing the paste with an equal volume of ethyl acetate three times, and discarding the ethyl acetate layer; (e) The aqueous layer was purified by macroporous adsorption resin column chromatography, eluted with 50% ethanol, and the eluate was concentrated under reduced pressure and spray-dried to obtain citronella flavonoids complex powder.

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