Composting bacteria capable of reducing antibiotics in composting process and composting method

Abstract

The invention discloses composting bacteria and a composting method capable of improving reduction of antibiotics in a composting process, relates to the technical field of composting, and aims to solve the problems that the traditional method has a limited reduction effect on the antibiotics, and antibiotic residues not only inhibit the microbial activity in the composting process, but also possibly enter soil through a compost product, so that the cost is reduced. The invention aims to solve the problem that the existing composting bacteria are used for solving the problem of influence on the balance of a soil ecosystem in the prior art, the composting bacteria are a composition of bacillus velezensis, bacillus subtilis, bacillus amyloliquefaciens and bacillus tequilensis, the proportions of the various strains are 15-25%, 25-35%, 25-35% and 15-25% in sequence, and the various strains are screened and optimized, so that the content of the bacillus amyloliquefaciens and the content of the bacillus tequilensis are increased. The strain has the capability of efficiently degrading antibiotics under a composting condition. The method has the advantages that antibiotics can be efficiently degraded in the composting process, and environmental pollution is reduced.

Description

Technical Field

[0001] The invention relates to the technical field of composting, and more particularly to a composting bacterium and a composting method capable of improving the reduction of antibiotics in the composting process. Background Art

[0002] With the rapid development of livestock and poultry farming, the treatment and utilization of manure has always been an important environmental issue in the livestock and poultry farming industry. Livestock and poultry manure not only contains a large amount of organic matter, but also carries pathogenic microorganisms, parasite eggs and antibiotic residues. If these harmful substances are not properly treated and directly applied to farmland, they will pollute the soil ecosystem and affect human health through the food chain.

[0003] Compost is an organic fertilizer made by mixing animal and plant remains and excrement containing fertilizer ingredients with soil and minerals, and then fermenting and decomposing them under high temperature and humid conditions. Compost is rich in nutrients and has a long and stable fertilizer effect. It is beneficial to the improvement of soil structure and can increase the soil's water retention, heat preservation, air permeability and fertilizer retention capabilities. Traditional composting methods mainly decompose the complex organic matter in livestock and poultry manure into humus through the fermentation of microorganisms, and at the same time generate high temperatures to kill pathogenic microorganisms. However, traditional methods have limited effects on the reduction of antibiotics. Antibiotic residues not only inhibit the activity of microorganisms during the composting process, but may also enter the soil through compost products, affecting the balance of the soil ecosystem.

[0004] In view of this, we propose a composting bacteria and a composting method that can improve the elimination of antibiotics during the composting process. Summary of the invention

[0005] The object of the present invention is to provide a composting bacteria and a composting method capable of improving the reduction of antibiotics during the composting process, aiming to solve the problem that the traditional method has limited effect on the reduction of antibiotics.

[0006] In order to solve the above technical problems, the present invention provides the following technical solutions: a composting bacterium capable of improving the reduction of antibiotics in the composting process, the composting bacterium is a combination of Bacillus Velez, Bacillus subtilis, Bacillus amyloliquefaciens and Bacillus tequila, the proportions of each strain are 15%-25%, 25%-35%, 25%-35%, 15%-25% in sequence, and each strain has been screened and optimized and has the ability to efficiently degrade antibiotics under composting conditions.

[0007] A composting method capable of improving the reduction of antibiotics during composting, the method using one of the above-mentioned composting bacteria capable of improving the reduction of antibiotics during composting to perform composting, the method comprising the following steps:

[0008] S1: Prepare raw materials: Select livestock and poultry manure containing antibiotic residues as the main composting raw material;

[0009] S2: Adding conditioners: Adding conditioners to livestock and poultry manure to adjust the carbon-nitrogen ratio and moisture content of the compost;

[0010] S3: adding a composting bacteria composition: evenly spreading the composting bacteria composition into the livestock and poultry manure;

[0011] S4: Compost management: compost the mixed manure, conditioner and composting bacteria composition, maintain appropriate humidity and ventilation conditions, and regularly monitor the temperature, humidity and pH value of the compost;

[0012] S5: Turning the pile and dissipating heat: During the high temperature period, the pile should be turned appropriately to dissipate heat and promote the entry of oxygen into the pile to maintain the activity of microorganisms;

[0013] S6: Composting: After the composting is completed, sufficient composting is carried out to ensure that harmful substances such as antibiotics are fully degraded.

[0014] Preferably, in the above step S2, the conditioning agent is a mixture of one or more of sawdust, straw and stalks, and the amount of the conditioning agent added is adjusted according to the water content and carbon-nitrogen ratio of livestock and poultry manure.

[0015] Preferably, in the above step S4, trace elements or minerals may be added as needed to further promote the growth and reproduction of microorganisms and improve composting efficiency.

[0016] Preferably, in the above step S4, the temperature monitoring range is 20°C-70°C, the humidity monitoring range is 40% to 60%, and the pH value monitoring range is 6.0 to 8.5.

[0017] Preferably, in the above step S5, an auxiliary agent is further added, wherein the auxiliary agent is one or a combination of biological enzymes or organic acids to improve the degradation efficiency of antibiotics and the compost quality.

[0018] Preferably, in the above step S6, it also includes conducting a quality inspection on the mature compost to evaluate indicators such as antibiotic residues, organic matter content, and nitrogen, phosphorus, and potassium content.

[0019] Preferably, in the above step S6, the compost product is also crushed, screened and packaged to meet the needs of different users and ensure the safety and stability of the product.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] 1. The composting bacteria of the present invention, which are composed of Bacillus Velez, Bacillus subtilis, Bacillus amyloliquefaciens and Bacillus tequila, are screened and optimized, and can efficiently degrade antibiotics in the composting process and reduce environmental pollution.

[0022] 2. The present invention selects livestock and poultry manure containing antibiotic residues as the main composting raw material, which not only solves the problem of livestock and poultry manure treatment, but also achieves the degradation of antibiotics, which is of environmental significance.

[0023] 3. The amount of the conditioner (sawdust, straw, stalks, etc.) added in the present invention is adjusted according to the water content and carbon-nitrogen ratio of livestock and poultry manure, so that the carbon-nitrogen ratio and moisture content of the compost reach the optimal state, which is conducive to the composting process.

[0024] 4. In the present invention, in the composting management, adding trace elements or minerals as needed can further promote the growth and reproduction of microorganisms and improve the composting efficiency.

[0025] 5. In the present invention, the temperature, humidity and pH value of the compost are regularly monitored to ensure that the composting process is carried out under suitable environmental conditions, which is conducive to the activity and degradation efficiency of microorganisms.

[0026] 6. In the present invention, the compost is properly turned during the high temperature period to dissipate heat and promote the entry of oxygen into the compost body, thereby maintaining the activity of microorganisms and preventing the compost from overheating and causing the death of microorganisms.

[0027] 7. After the composting is completed, the present invention performs a sufficient decomposition treatment to ensure that harmful substances such as antibiotics are completely degraded. At the same time, the quality of the decomposed compost is tested to evaluate the antibiotic residue, organic matter content, nitrogen, phosphorus and potassium content and other indicators to ensure the quality of the compost. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 The figure is a schematic diagram of the composting method of the present invention. DETAILED DESCRIPTION

[0029] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0030] The invention can efficiently degrade antibiotics, has a scientific strain ratio, reasonable raw material selection, flexible addition of conditioners, addition of trace elements or minerals to promote microbial growth, environmental monitoring and control to ensure a suitable environment, compost turning and heat dissipation to maintain microbial activity, composting treatment and quality inspection to ensure compost quality, product processing and packaging to meet user needs and ensure safety and stability. These advantages make the composting method have broad application prospects in treating livestock and poultry manure containing antibiotic residues

[0031] Embodiment 1

[0032] like Figure 1 As shown, the present invention relates to a composting bacteria capable of improving the reduction of antibiotics in the composting process. The composting bacteria is a composition of Bacillus Velez, Bacillus subtilis, Bacillus amyloliquefaciens and Bacillus tequila, and the proportions of the strains are 15%, 35%, 35% and 15% in sequence, and each strain has been screened and optimized and has the ability to efficiently degrade antibiotics under composting conditions.

[0033] A composting method capable of improving the reduction of antibiotics during composting, the method using one of the above-mentioned composting bacteria capable of improving the reduction of antibiotics during composting to perform composting, the method comprising the following steps:

[0034] S1: Prepare raw materials: Select livestock and poultry manure containing antibiotic residues as the main composting raw material;

[0035] S2: Adding conditioners: Adding conditioners to livestock and poultry manure to adjust the carbon-nitrogen ratio and moisture content of the compost;

[0036] S3: adding a composting bacteria composition: evenly spreading the composting bacteria composition into the livestock and poultry manure;

[0037] S4: Compost management: compost the mixed manure, conditioner and composting bacteria composition, maintain appropriate humidity and ventilation conditions, and regularly monitor the temperature, humidity and pH value of the compost;

[0038] S5: Turning the pile and dissipating heat: During the high temperature period, the pile should be turned appropriately to dissipate heat and promote the entry of oxygen into the pile to maintain the activity of microorganisms;

[0039] S6: Composting: After the composting is completed, it is fully decomposed to ensure that harmful substances such as antibiotics are fully degraded;

[0040] In the above step S2, the conditioning agent is a mixture of one or more of sawdust, straw, and stalks, and the amount of the conditioning agent added is adjusted according to the water content and carbon-nitrogen ratio of livestock and poultry manure;

[0041] In the above step S4, trace elements or minerals may be added as needed to further promote the growth and reproduction of microorganisms and improve composting efficiency;

[0042] In the above step S4, the temperature monitoring range is 20°C-70°C, the humidity monitoring range is 40% to 60%, and the pH value monitoring range is 6.0 to 8.5;

[0043] In the above step S5, it also includes adding an auxiliary agent, wherein the auxiliary agent is one or a combination of biological enzymes or organic acids to improve the degradation efficiency of antibiotics and the compost quality;

[0044] In the above step S6, it also includes testing the quality of the mature compost to evaluate the antibiotic residue, organic matter content, nitrogen, phosphorus and potassium content and other indicators;

[0045] In the above step S6, the compost product is also crushed, screened and packaged to meet the needs of different users and ensure the safety and stability of the product.

[0046] Embodiment 2

[0047] A composting bacterium capable of improving the reduction of antibiotics during composting. The composting bacterium is a combination of Bacillus Velez, Bacillus subtilis, Bacillus amyloliquefaciens and Bacillus tequila, wherein the proportions of the various strains are 20%, 30%, 30% and 20% in sequence, and each strain has been screened and optimized and has the ability to efficiently degrade antibiotics under composting conditions.

[0048] A composting method capable of improving the reduction of antibiotics during composting, the method using one of the above-mentioned composting bacteria capable of improving the reduction of antibiotics during composting to perform composting, the method comprising the following steps:

[0049] S1: Prepare raw materials: Select livestock and poultry manure containing antibiotic residues as the main composting raw material;

[0050] S2: Adding conditioners: Adding conditioners to livestock and poultry manure to adjust the carbon-nitrogen ratio and moisture content of the compost;

[0051] S3: adding a composting bacteria composition: evenly spreading the composting bacteria composition into the livestock and poultry manure;

[0052] S4: Compost management: compost the mixed manure, conditioner and composting bacteria composition, maintain appropriate humidity and ventilation conditions, and regularly monitor the temperature, humidity and pH value of the compost;

[0053] S5: Turning the pile and dissipating heat: During the high temperature period, the pile should be turned appropriately to dissipate heat and promote the entry of oxygen into the pile to maintain the activity of microorganisms;

[0054] S6: Composting: After the composting is completed, it is fully decomposed to ensure that harmful substances such as antibiotics are fully degraded;

[0055] In the above step S2, the conditioning agent is a mixture of one or more of sawdust, straw, and stalks, and the amount of the conditioning agent added is adjusted according to the water content and carbon-nitrogen ratio of livestock and poultry manure;

[0056] In the above step S4, trace elements or minerals may be added as needed to further promote the growth and reproduction of microorganisms and improve composting efficiency;

[0057] In the above step S4, the temperature monitoring range is 20°C-70°C, the humidity monitoring range is 40% to 60%, and the pH value monitoring range is 6.0 to 8.5;

[0058] In the above step S5, it also includes adding an auxiliary agent, wherein the auxiliary agent is one or a combination of biological enzymes or organic acids to improve the degradation efficiency of antibiotics and the compost quality;

[0059] In the above step S6, it also includes testing the quality of the mature compost to evaluate the antibiotic residue, organic matter content, nitrogen, phosphorus and potassium content and other indicators;

[0060] In the above step S6, the compost product is also crushed, screened and packaged to meet the needs of different users and ensure the safety and stability of the product;

[0061] This embodiment is basically the same as the first embodiment, except that the ratios of the various bacteria species used to form the composting bacteria are different.

[0062] Embodiment 3

[0063] A composting bacterium capable of improving the reduction of antibiotics during composting. The composting bacterium is a combination of Bacillus velez, Bacillus subtilis, Bacillus amyloliquefaciens and Bacillus tequila, wherein the proportions of the various strains are 25%, 25%, 25% and 25% in sequence, and each strain has been screened and optimized and has the ability to efficiently degrade antibiotics under composting conditions.

[0064] A composting method capable of improving the reduction of antibiotics during composting, the method using one of the above-mentioned composting bacteria capable of improving the reduction of antibiotics during composting to perform composting, the method comprising the following steps:

[0065] S1: Prepare raw materials: Select livestock and poultry manure containing antibiotic residues as the main composting raw material;

[0066] S2: Adding conditioners: Adding conditioners to livestock and poultry manure to adjust the carbon-nitrogen ratio and moisture content of the compost;

[0067] S3: adding a composting bacteria composition: evenly spreading the composting bacteria composition into the livestock and poultry manure;

[0068] S4: Compost management: compost the mixed manure, conditioner and composting bacteria composition, maintain appropriate humidity and ventilation conditions, and regularly monitor the temperature, humidity and pH value of the compost;

[0069] S5: Turning the pile and dissipating heat: During the high temperature period, the pile should be turned appropriately to dissipate heat and promote the entry of oxygen into the pile to maintain the activity of microorganisms;

[0070] S6: Composting: After the composting is completed, it is fully decomposed to ensure that harmful substances such as antibiotics are fully degraded;

[0071] In the above step S2, the conditioning agent is a mixture of one or more of sawdust, straw, and stalks, and the amount of the conditioning agent added is adjusted according to the water content and carbon-nitrogen ratio of livestock and poultry manure;

[0072] In the above step S4, trace elements or minerals may be added as needed to further promote the growth and reproduction of microorganisms and improve composting efficiency;

[0073] In the above step S4, the temperature monitoring range is 20°C-70°C, the humidity monitoring range is 40% to 60%, and the pH value monitoring range is 6.0 to 8.5;

[0074] In the above step S5, it also includes adding an auxiliary agent, wherein the auxiliary agent is one or a combination of biological enzymes or organic acids to improve the degradation efficiency of antibiotics and the compost quality;

[0075] In the above step S6, it also includes testing the quality of the mature compost to evaluate the antibiotic residue, organic matter content, nitrogen, phosphorus and potassium content and other indicators;

[0076] In the above step S6, the compost product is also crushed, screened and packaged to meet the needs of different users and ensure the safety and stability of the product;

[0077] This embodiment is basically the same as the first embodiment, except that the ratios of the various bacteria species used to form the composting bacteria are different.

[0078] Comparative Example 1

[0079] This comparative example is basically the same as Example 1, except that during the composting process of this comparative example, the temperature monitoring range of the compost is 30° C.-60° C.

[0080] Comparative Example 2

[0081] This comparative example is substantially the same as the first embodiment, except that during the composting process of this comparative example, the humidity monitoring range of the compost is 45% to 65%.

[0082] Comparative Example 3

[0083] This comparative example is basically the same as Example 1, except that during the composting process of this comparative example, the pH value monitoring range of the compost is 7.0 to 9.

[0084] The antibiotic content in the compost obtained from the above Examples 1 to 3 and Comparative Examples 1 to 3 was tested, and the specific results can be referred to the following table:

[0085]

[0086] The embodiments of the present invention disclose preferred embodiments, but are not limited thereto. A person skilled in the art can easily understand the spirit of the present invention based on the above embodiments and make different extensions and changes. However, as long as they do not deviate from the spirit of the present invention, they are all within the protection scope of the present invention.

Claims

1. A composting bacterium capable of improving the consumption of antibiotics during composting, characterized in that: The composting bacteria is a combination of Bacillus velez, Bacillus subtilis, Bacillus amyloliquefaciens and Bacillus tequila, wherein the proportions of the bacteria species are 15%-25%, 25%-35%, 25%-35% and 15%-25% in sequence, and each bacteria species has been screened and optimized and has the ability to efficiently degrade antibiotics under composting conditions.

2. A composting method capable of improving the reduction of antibiotics during the composting process, the method using the composting bacteria capable of improving the reduction of antibiotics during the composting process as claimed in claim 1 for composting, characterized in that: The method comprises the following steps: S1: Prepare raw materials: Select livestock and poultry manure containing antibiotic residues as the main composting raw material; S2: Adding conditioners: Adding conditioners to livestock and poultry manure to adjust the carbon-nitrogen ratio and moisture content of the compost; S3: adding a composting bacteria composition: evenly spreading the composting bacteria composition into the livestock and poultry manure; S4: Compost management: compost the mixed manure, conditioner and composting bacteria composition, maintain appropriate humidity and ventilation conditions, and regularly monitor the temperature, humidity and pH value of the compost; S5: Turning the pile and dissipating heat: During the high temperature period, the pile should be turned appropriately to dissipate heat and promote the entry of oxygen into the pile to maintain the activity of microorganisms; S6: Composting: After the composting is completed, sufficient composting is carried out to ensure that harmful substances such as antibiotics are fully degraded.

3. A composting method capable of improving the elimination of antibiotics during composting according to claim 2, characterized in that: In the above step S2, the conditioning agent is a mixture of one or more of sawdust, straw, and stalks, and the amount of the conditioning agent added is adjusted according to the water content and carbon-nitrogen ratio of livestock and poultry manure.

4. A composting method capable of improving the elimination of antibiotics during composting according to claim 2, characterized in that: In the above step S4, trace elements or minerals may be added as needed to further promote the growth and reproduction of microorganisms and improve composting efficiency.

5. A composting method capable of improving the elimination of antibiotics during composting according to claim 2, characterized in that: In the above step S4, the temperature monitoring range is 20°C-70°C, the humidity monitoring range is 40% to 60%, and the pH value monitoring range is 6.0 to 8.

5.

6. A composting method capable of improving the elimination of antibiotics during composting according to claim 2, characterized in that: In the above step S5, it also includes adding an auxiliary agent, which is one or a combination of biological enzymes or organic acids to improve the degradation efficiency of antibiotics and the compost quality.

7. A composting method capable of improving the elimination of antibiotics during composting according to claim 2, characterized in that: In the above step S6, it also includes conducting quality inspection on the mature compost to evaluate indicators such as antibiotic residue, organic matter content, nitrogen, phosphorus and potassium content.

8. A composting method capable of improving the elimination of antibiotics during composting according to claim 2, characterized in that: In the above step S6, the compost product is also crushed, screened and packaged to meet the needs of different users and ensure the safety and stability of the product.

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