A mouse heart magnetocardiography magnetoencephalography testing device and method based on zero field atomic magnetometer

Abstract

The application relates to a mouse heart magnetocardiography and magnetoencephalography testing device and method based on a zero-field atomic magnetometer, and relates to the field of heart magnetocardiography and magnetoencephalography testing. The application solves the problems that the existing mouse heart magnetocardiography and magnetoencephalography testing device has a large volume, and the magnetic field signal is distorted due to the movement or struggle of the mouse, and motion artifacts are generated. A plurality of groups of tool fixing hole positions (1-1) are formed in pairs on the upper end face of a platform (1), a plurality of heart magnetometer mounting holes (1-2) are formed in the middle of the length direction of the lower end face of the platform (1), a heart magnetometer is mounted in the heart magnetometer mounting hole (1-2), one insert (2) is inserted in each group of tool fixing hole positions (1-1), the insert (2) is provided with a magnetoencephalography mounting hole (2-1) and a head mounting hole (2-2). The mouse heart magnetic signal and the magnetoencephalography signal are tested at the same time, and the error introduced by the environmental magnetic field change in the experiment process is reduced to the maximum extent. The application is used for mouse heart magnetocardiography and magnetoencephalography testing.

Description

Technical Field

[0001] The present invention relates to a device and method for testing cardio-encephalogram magnetism, and in particular to a device and method for testing cardio-encephalogram magnetism in mice based on a zero-field atomic magnetometer. Background Art

[0002] In recent years, biomagnetic field measurement technology has developed rapidly, particularly with advances in superconducting quantum interference devices (SQUIDs), which have made it possible to measure weak biomagnetic field signals. This technology holds broad application prospects in fields such as neuroscience, cardiovascular disease research, and drug screening. Currently, biomagnetic field measurement technology is primarily used in humans, while biomagnetic field measurement in small animals such as mice is still in its infancy. Due to the tiny size of mice, the magnetic field signals generated by their hearts and brains are extremely weak, making them difficult to detect using traditional biomagnetic field measurement instruments.

[0003] The mouse's magnetic heart and brain signals are extremely weak magnetic fields generated by heart and brain activity, respectively. Unlike humans and other large mammals, the magnetic fields generated by the mouse heart and brain are extremely weak, typically only on the order of picotesla (pT) or even lower. These signals are susceptible to environmental magnetic fields, the organism's own magnetic field, and other interference factors. Therefore, measuring and analyzing these signals requires rigorous experimental conditions and sophisticated instruments.

[0004] Mouse magnetic heart signals can be used to study the heart's electrical and mechanical activity, as well as its hemodynamics, providing new insights and methods for cardiovascular disease research. Furthermore, magnetic heart signals can be used as a drug screening indicator to evaluate the effects of drugs on cardiac function and accelerate drug development. Furthermore, mouse magnetic heart signals can serve as a model for biomagnetic field research, providing new insights into the mechanisms, applications, and significance of biomagnetic fields.

[0005] Mouse brain magnetic signals can be used to study the brain's electrical activity, cognitive function, learning and memory, and other information, providing new ideas and methods for neuroscience research. Mouse brain magnetic signals can also be used to study various brain disease models, such as Alzheimer's disease, Parkinson's disease, and epilepsy, providing new evidence for disease diagnosis and treatment. In addition, mouse brain magnetic signals can serve as a model for biomagnetic field research, providing new insights into the generation mechanism, application, and significance of biomagnetic fields.

[0006] In the traditional test process of mouse magnetic heart and brain signals, due to the limitations of the test equipment, the magnetic heart and brain signals are tested separately, which greatly increases the test time. At the same time, due to the large size of existing biomagnetic field measuring instruments, it is difficult to meet the needs of measuring mice. The test device is usually selected as a superconducting quantum interference device (SQUID) system, which is widely used in biomagnetic field measurement due to its advantages such as ultra-high sensitivity, low noise, and wide bandwidth. However, its low temperature operating environment and large size and weight limit its application in some environments. In addition, during the measurement of mouse magnetic heart and brain magnetism, the movement or struggle of the mouse causes distortion of the magnetic field signal and produces motion artifacts. It is necessary to ensure the stability of the mouse during the measurement process.

[0007] In summary, existing mouse magnetocardiography and magnetoencephalography measurement devices have the problems of large instrument size, magnetic field signal distortion caused by the movement or struggle of the mouse, and motion artifacts. Summary of the Invention

[0008] The present invention aims to address the issues of existing mouse magnetocardiography and brain magnetometry measurement devices, such as large instrument size, magnetic field signal distortion caused by the mouse's movement or struggle, and motion artifacts. Furthermore, a device and method for measuring mouse magnetocardiography and brain magnetometry based on a zero-field atomic magnetometer are provided.

[0009] The technical solution of the present invention is:

[0010] A mouse cardio-brain magnetometry testing device based on a zero-field atomic magnetometer comprises a zero-field atomic magnetometer, a platform, multiple plug-ins, multiple brain magnetometry magnetometers, and multiple cardio-brain magnetometers.

[0011] The upper end surface of the platform is provided with multiple groups of tool fixing holes in pairs, and the lower end surface of the platform is provided with multiple cardiomagnetic magnetometer mounting holes along the middle of its length direction. The cardiomagnetic magnetometer is installed in the cardiomagnetic magnetometer mounting holes. A plug-in is inserted into each group of tool fixing holes, and the plug-in is provided with a brain magnetometer mounting hole and a head mounting hole; the platform, multiple plug-ins, multiple brain magnetometers and multiple cardiomagnetic magnetometers are all placed in the zero magnetic device.

[0012] Furthermore, the tool fixing hole is a rectangular hole.

[0013] Preferably, the plurality of core magnetometer mounting holes are arranged at equal intervals.

[0014] Furthermore, a cable trough is processed on the lower end surface of the platform along its length direction, and the cable trough is connected to the end of the plurality of core magnetometer mounting holes.

[0015] Furthermore, the distance between the bottom of the mounting hole of the brain magnetometer and the upper end surface of the platform is L, L=1.5-3mm.

[0016] Furthermore, the plug-in includes two rectangular legs, a connector and a slot body, the slot body is installed on the upper end surface of the connector, and the brain magnetometer mounting hole and the head mounting hole are independently opened in the slot body, the two rectangular legs are installed on the lower end surface of the connector, and the two rectangular legs are inserted into the tooling fixing holes.

[0017] Preferably, the lengths of the connectors between the multiple plug-ins gradually decrease from left to right or from right to left.

[0018] Furthermore, a plurality of threaded holes are respectively processed on both sides of the upper end surface of the platform in the length direction.

[0019] The present invention also provides a testing method using a mouse magnetocardioencephalography testing device based on a zero-field atomic magnetometer, which comprises the following steps:

[0020] Step 1: Fix the mouse's head and perform brain magnetic testing;

[0021] Step 1: After the mouse is anesthetized, place it on the platform of the mouse magnetocardioencephalometric testing device based on a zero-field atomic magnetometer;

[0022] Step 1 and 2: Select a plug-in of appropriate mouse size on the platform and insert it into the corresponding tooling fixing hole on the platform;

[0023] Step 13: Fix the mouse's head in the head mounting hole, select the EEG magnetometer mounting hole located just above the mouse's brain in the plug-in, insert the EEG magnetometer into the mounting hole, and measure the mouse's brain magnetism;

[0024] Step 2: Fix the mouse's limbs;

[0025] Insert screws into the corresponding positions of the mouse's limbs on both sides of the platform, and use four thin wires to fix one end to the mouse's limbs, and the other ends of the four thin wires to fix the screws;

[0026] Step 3: Performing magnetic cardiology testing on mice;

[0027] Select the cardiac magnetometry mounting hole at the bottom of the platform, which is directly below the mouse heart. Insert the cardiac magnetometry into the selected mounting hole to measure the mouse cardiac magnetism. Pass the cable through the cable groove at the bottom of the platform.

[0028] Step 4: By operating the zero-field atomic magnetometer software outside the shielding device, the test of the mouse's brain magnetic signals and heart magnetic signals can be completed.

[0029] Furthermore, to ensure the accuracy of the test results, the test process from step 1 to step 4 is performed in a zero-magnetic environment of a magnetic shielding room or a magnetic shielding barrel.

[0030] Compared with the prior art, the present invention has the following effects:

[0031] 1. The present invention provides a mouse magnetocardiogram and brain magnetometry testing device, and places multiple brain magnetocardiogram magnetometers and multiple heart magnetocardiogram magnetometers in the testing device at the same time. This can solve the problem in the prior art that the mouse magnetocardiogram and brain magnetometry signals cannot be tested simultaneously, thereby reducing the testing time and minimizing the errors introduced by changes in the environmental magnetic field during the experiment.

[0032] 2. The present invention limits the length of connectors 2-4 in the plug-in, allowing different plug-ins to have different sizes to accommodate mice of different sizes. This solves the problem of individual differences in mice, where a single device cannot accommodate the placement requirements of a zero-field atomic magnetometer (QZFM) for magnetoencephalography (MGE) testing of mice of varying sizes. Furthermore, the present invention addresses the problem of distorted MGE or MGE signals caused by twitching or struggling during testing, ensuring mouse stability and comfort during the measurement process, and improving experimental efficiency and data reliability.

[0033] 3. The present invention uses a magnetic shielding device and a high-precision magnetic sensor QZFM to minimize the influence of external interference signals such as the external magnetic field environment and the test instrument's own noise.

[0034] 4. The entire device is made of ABS plastic, which offers high strength, resistance to deformation, good workability, small size, light weight, and ease of operation. Furthermore, nylon is non-magnetic, avoiding interference from additional magnetic fields and making it suitable for testing in near-zero magnetic environments within shielded rooms or shielded cylinders.

[0035] 5. This invention will provide a new technical means for measuring cardiac magnetometry and brain magnetometry in mice, and will also provide new research tools and methods for fields such as neuroscience, cardiovascular disease research, and drug screening, thereby promoting the development of related fields.

[0036] 6. The zero-field magnetometer (QZFM) of the present invention is an ultra-sensitive vector magnetometer that operates in a low-field environment. It has performance comparable to superconducting technology and is a modern sensor for brain magnetometry and heart magnetometry. The sensor head operates at room temperature and fully integrates the laser with other components, eliminating the complexity of optical fiber or cryogenics. Each QZFM is self-calibrated and can measure field components on three axes simultaneously. A compact electronic module with a microprocessor provides all the necessary functions, and the sensor can be operated with just a few clicks of the mouse. Compared with less sensitive fluxgates and superconducting quantum interference devices with harsh operating conditions, its ultra-high sensitivity in the femtosecond range, small size and recommended operating procedures, very low noise level, and the principle of atomic spin response to external magnetic fields enable it to be widely used in materials science, biomedicine, geophysics and other fields.

[0037] 7. This invention is a QZFM-based device and method for measuring mouse magnetocardiography and brain magnetometry, designed to address the problem of inaccurate measurement of mouse magnetocardiography and brain magnetometry signals. By incorporating QZFM as a highly sensitive magnetic sensor, high-precision measurement and analysis of mouse magnetocardiography and brain magnetometry signals can be achieved. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] Figure 1 It is the overall structural diagram of the present invention; Figure 2 is a schematic diagram of the three-dimensional structure of platform 1; Figure 3 is a bottom view of platform 1; Figure 4 is a top view of platform 1; Figure 5 yes Figure 4 Cross-sectional view along AA; Figure 6 yes Figure 4 Cross-sectional view along BB; Figure 7 It is a schematic diagram of the three-dimensional structure of plug-in 2; Figure 8 This is a schematic diagram of the structure of a zero-field atomic magnetometer.

[0039] In the figure: 1. Platform, 1-1. Fixture fixing hole, 1-2. Magnetocentrometer mounting hole, 1-3. Cable trough, 2. Plug-in, 2-1. Magnetocentrometer mounting hole, 2-2. Head mounting hole, 2-3. Rectangular leg, 2-4. Connector, 2-5. Trough. DETAILED DESCRIPTION

[0040] Specific implementation method 1: Combination Figures 1 to 8 To illustrate this embodiment, this embodiment includes a zero-magnetic device, a platform 1, a plurality of plug-ins 2, a plurality of brain magnetometers, and a plurality of heart magnetometers;

[0041] The upper end surface of the platform 1 is provided with a plurality of tool fixing holes 1-1 in pairs, and the lower end surface of the platform 1 is provided with a plurality of cardiomagnetic magnetometer mounting holes 1-2 along the middle of its length direction. The cardiomagnetic magnetometer is installed in the cardiomagnetic magnetometer mounting holes 1-2. A plug-in 2 is inserted into each set of tool fixing holes 1-1, and the plug-in 2 is provided with a brain magnetometer mounting hole 2-1 and a head mounting hole 2-2; the platform 1, the plurality of plug-ins 2, the plurality of brain magnetometers and the plurality of cardiomagnetic magnetometers are all placed in the zero magnetic device.

[0042] The plug-in 2 in this embodiment plays a role in fixing the mouse's head and placing the QZFM for the mouse brain magnetic test. The platform 1 is used to place the mouse and the QZFM for the mouse brain magnetic test.

[0043] The zero-magnetic device in this embodiment is a novel magnetically shielded chamber that typically combines passive shielding and active compensation technologies to achieve an ultra-low magnetic field environment close to zero (<1nT). Passive shielding typically utilizes multiple layers of highly permeable magnetic materials (such as Permalloy), achieving shielding effectiveness exceeding 120dB. The zero-magnetic device provides a fundamental research platform for the study and application of weak magnetic fields and is widely used in fundamental physics and life science research.

[0044] Advantages of the magnetic shielding device (i.e., zero magnetic device) in the present invention:

[0045] 1. Extremely low background interference: through the combination of active shielding and passive shielding (①. The principle of active shielding: the change of the environmental magnetic field is detected in real time by the sensor, and the reverse magnetic field is generated by the electromagnetic coil system to dynamically offset the external magnetic field interference. ② Passive shielding principle: the physical properties of high magnetic permeability materials (such as μ metal and Permalloy) are used to absorb or guide the magnetic flux lines to bypass the protected area. ③ The combination of the two: In actual zero-magnetic devices, active and passive shielding are usually layered and cooperated to form "double protection": First: outer passive shielding: Use multiple layers of high magnetic permeability materials (such as μ metal) and conductive layers (such as aluminum) to form a physical barrier to weaken most external magnetic fields (especially high-frequency and strong magnetic fields). For example: build the walls of a magnetic shielding room to absorb or reflect the outside world Magnetic field. Second: Inner active shielding: An electromagnetic coil array is arranged inside the passive shielding, and the residual magnetic field is detected through a real-time feedback system to generate a reverse magnetic field to offset the residual interference. For example: In magnetoencephalography (MEG) equipment, active compensation coils can eliminate tiny magnetic field fluctuations caused by human breathing or equipment vibration. Third: Synergistic advantages: Complementary frequency bands: Passive shielding is good at handling high frequencies and strong magnetic fields, while active shielding targets low frequencies and dynamic interference. Anti-saturation ability: When the passive material is close to saturation due to a strong magnetic field, the active system can intervene to compensate. Flexibility and precision: The active system can dynamically adapt to environmental changes, and the passive system provides basic protection. ), the internal residual magnetic field and magnetic field noise are extremely low, with a clean and stable magnetic field environment, which can detect weaker biomagnetic signals. Without low-frequency interference, the time-frequency characteristics of the cardio-cerebromagnetic signal are clearer, which is conducive to identifying subtle pathological changes.

[0046] 2. The internal space of the zero-magnetic device is larger than that of a traditional magnetic shielding barrel (typically a cube greater than 1.5*1.5*1.5m), and it can achieve a large, uniform zero-magnetic zone, making it easy to operate and suitable for multi-sensor arrays or experiments with freely moving animals. It can be designed as an open or large cavity, facilitating the integration of multimodal equipment such as optical and electrophysiological devices.

[0047] 3. Improved signal-to-noise ratio of zero-magnetic devices: Cardiac magnetic signals (about 1pT) and brain magnetic signals (about 0.1-1fT) are extremely weak. The zero-magnetic environment enables high-sensitivity magnetometers (such as SQUID and atomic magnetometers) to capture more realistic physiological activities.

[0048] 4. The magnetic shielding chamber can simulate the day and night temperature environment changes by adjusting the light and temperature inside the magnetic shielding chamber.

[0049] Specific implementation method two: combined with Figures 1 to 6 In this embodiment, the tool fixing hole 1-1 is a rectangular hole. In this way, the plug-in can be inserted into the tool fixing hole 1-1 during testing. The rectangular hole can prevent the plug-in from rotating and ensure the stability of the mouse fixation under the premise of ensuring the connection. The other components and connection relationships are the same as those in the first specific implementation method.

[0050] Specific implementation method three: combined with Figure 3 In this embodiment, the multiple magnetocardiography magnetometers mounting holes 1-2 are arranged at equal intervals. In this way, the appropriate position can be selected for installing the QZFM according to actual needs. The other components and connection relationships are the same as those in the first or second specific implementation method.

[0051] As a preferred embodiment, the number of magnetocardiography magnetometer mounting holes 1-2 is 6 or 8 or 10. This can facilitate the placement of the magnetocardiography magnetometer at the heart position of the mouse, ensuring the accuracy of the magnetocardiography measurement.

[0052] The bottom of the optimal platform 1 has eight QZFM placement positions. During the magnetocardiography test, the most suitable placement position can be selected to ensure that the mouse heart is located directly above the QZFM.

[0053] Specific implementation method four: combined with Figure 3 In this embodiment, the lower end surface of the platform 1 is also processed with a cable groove 1-3 along its length direction, and the cable groove 1-3 is in communication with the first and last of the multiple magnetocardiography magnetometer mounting holes 1-2. In this way, the QZFM cable passes through the bottom of the platform to ensure the stability of the entire tool during testing. The other components and connection relationships are the same as those in any one of the first to third specific implementation methods.

[0054] Specific implementation method five: combined with Figure 5 In this embodiment, the distance between the hole bottom of the magnetoencephalography magnetometer mounting hole 2-1 and the upper end surface of the platform 1 is L, and L = 1.5-3 mm.

[0055] In this way, the distance between the hole bottom of the magnetoencephalography magnetometer mounting hole 2-1 and the upper end surface of the platform 1 plays an important role in the accuracy of the magnetocardiography measurement, and the preferred distance is 1.8 mm, which is the most accurate for magnetocardiography measurement. The other structures and components are the same as those in any one of the first to fourth specific implementation methods.

[0056] Specific implementation method six: combined with Figure 1 and Figure 6To illustrate this embodiment, the plug-in 2 of this embodiment includes two rectangular legs 2-3, a connector 2-4 and a slot 2-5. The slot 2-5 is installed on the upper end surface of the connector 2-4, and the brain magnetometer mounting hole 2-1 and the head mounting hole 2-2 are independently opened in the slot 2-5. The two rectangular legs 2-3 are installed on the lower end surface of the connector 2-4, and the two rectangular legs 2-3 are inserted into the tooling fixing hole 1-1.

[0057] This arrangement is simple in structure and easy to implement, and can provide installation space for both magnetocardiography and magnetoencephalography testing. The other components and connection relationships are the same as any one of the specific embodiments 1 to 5.

[0058] Specific implementation method seven: combination Figure 1 To illustrate this embodiment, the lengths of the connectors 2 - 4 between the multiple plug-ins 2 in this embodiment gradually decrease from left to right or from right to left.

[0059] In this way, in order to adapt to the size of different mice, three different sizes of plug-ins are designed. When testing, the most suitable size plug-in can be selected. The other components and connection relationships are the same as any one of the specific embodiments 1 to 6.

[0060] Specific implementation method eight: combination Figures 1 to 6 To describe this embodiment, a plurality of threaded holes 1 - 4 are processed on both sides of the upper end surface of the platform 1 in the longitudinal direction.

[0061] With this setup, the platform has M4 threaded holes on both sides. During testing, screws are screwed into these holes. A thin wire is then attached to each of the mouse's limbs, with the other end secured to the screws. This secures the mouse during testing. The remaining components and connections are the same as in any of the first through seventh embodiments.

[0062] Specific implementation method nine: combination Figures 1 to 8 The test method of this embodiment includes the following steps:

[0063] Step 1: Fix the mouse's head and perform brain magnetic testing;

[0064] Step 1: After the mouse is anesthetized, place it on platform 1 of the mouse magnetocardioencephalography testing device based on a zero-field atomic magnetometer;

[0065] Step 1-2: Select a plug-in 2 of appropriate mouse size on platform 1 and insert it into the corresponding tooling fixing hole 1-1 of platform 1;

[0066] Step 1-3: Fix the mouse's head in the head mounting hole 2-2, select the brain magnetometer mounting hole 2-1 located just above the mouse's brain in plug-in 2, insert the brain magnetometer into the brain magnetometer mounting hole 2-1, and measure the mouse's brain magnetism;

[0067] Step 2: Fix the mouse's limbs;

[0068] Insert screws into the corresponding positions of the mouse's limbs on both sides of platform 1, and use one end of four thin wires to fix the mouse's limbs, and the other ends of the four thin wires to fix the screws;

[0069] Step 3: Performing magnetic cardiology testing on mice;

[0070] Select the cardiac magnetometer mounting hole 1-2 at the bottom of platform 1, which is located directly below the mouse heart. Insert the cardiac magnetometer into the selected cardiac magnetometer mounting hole 1-2 to measure the mouse cardiac magnetism. Pass the cable through the cable slot 1-4 at the bottom of platform 1.

[0071] Step 4: By operating the zero-field atomic magnetometer software outside the shielding device, the test of the mouse's brain magnetic signals and heart magnetic signals can be completed.

[0072] Specific implementation method ten: Combination Figures 1 to 7 To illustrate this embodiment, in order to ensure the accuracy of the test results, the test process from step 1 to step 4 is performed in a zero-magnetic environment of a magnetic shielding room or a magnetic shielding barrel.

[0073] This arrangement is convenient for ensuring that the measurement process is not affected by the outside world. Other components and connection relationships are the same as any one of the specific implementation methods 1 to 9.

[0074] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the various embodiments of the present invention.

Claims

1. A device for testing mouse magnetocardiography and brain magnetometry based on a zero-field atomic magnetometer, characterized by: The device comprises a zero magnetic device, a platform (1), a plurality of plug-ins (2), a plurality of brain magnetometers and a plurality of heart magnetometers; The upper end surface of the platform (1) is provided with a plurality of tool fixing holes (1-1) in pairs, and the lower end surface of the platform (1) is provided with a plurality of heart magnetometer mounting holes (1-2) along the middle of its length direction. The heart magnetometer is installed in the heart magnetometer mounting hole (1-2), and a plug-in (2) is inserted in each set of tool fixing holes (1-1). The plug-in (2) is provided with a brain magnetometer mounting hole (2-1) and a head mounting hole (2-2); the platform (1), the plurality of plug-ins (2), the plurality of brain magnetometers and the plurality of heart magnetometers are all placed in the zero magnetic device; A plurality of centroid magnetometer mounting holes (1-2) are arranged at equal intervals; The lower end surface of the platform (1) is further processed with a cable groove (1-3) along its length direction, and the cable groove (1-3) is connected to the end of a plurality of core magnetometer mounting holes (1-2); The distance between the bottom of the brain magnetometer mounting hole (2-1) and the upper end surface of the platform (1) is L, L = 1.5-3 mm; The plug-in (2) includes two rectangular legs (2-3), a connector (2-4) and a slot (2-5), the slot (2-5) is mounted on the upper end surface of the connector (2-4), and the brain magnetometer mounting hole (2-1) and the head mounting hole (2-2) are independently opened in the slot (2-5), the two rectangular legs (2-3) are mounted on the lower end surface of the connector (2-4), and the two rectangular legs (2-3) are inserted into the tooling fixing hole (1-1); The lengths of the connectors (2-4) between the multiple plug-ins (2) gradually decrease from left to right or from right to left.

2. The device for testing mouse magnetocardiography and brain magnetometry based on a zero-field atomic magnetometer according to claim 1, characterized in that: The tool fixing hole (1-1) is a rectangular hole.

3. The device for testing mouse magnetocardiography and brain magnetometry based on a zero-field atomic magnetometer according to claim 1, characterized in that: A plurality of threaded holes (1-4) are respectively processed on both sides of the upper end surface of the platform (1) in the length direction.

4. A testing method using the mouse magnetocardioencephalography testing device based on a zero-field atomic magnetometer according to any one of claims 1 to 3, characterized in that: It includes the following steps: Step 1: Fix the mouse's head and perform brain magnetic testing; Step 1: Place the mouse on the platform (1) of the mouse magnetocardioencephalography testing device based on a zero-field atomic magnetometer; Step 1 and 2: Select a plug-in (2) of appropriate mouse size on the platform (1), and insert the plug-in (2) into the corresponding tool fixing hole (1-1) of the platform (1); Step 1-3: Fix the mouse head in the head mounting hole (2-2), select the brain magnetometer mounting hole (2-1) located just above the mouse brain in the plug-in (2), insert the brain magnetometer into the brain magnetometer mounting hole (2-1), and measure the mouse brain magnetism; Step 2: Fix the mouse's limbs; Insert screws into the corresponding positions of the mouse's limbs on both sides of the platform (1), and use one end of four thin wires to fix the mouse's limbs respectively, and the other ends of the four thin wires are fixed to the screws respectively; Step 3: Performing magnetic cardiology testing on mice; Select the cardiac magnetometry mounting hole (1-2) located at the bottom of the platform (1) just below the mouse heart, insert the cardiac magnetometry into the selected cardiac magnetometry mounting hole (1-2), and measure the cardiac magnetism of the mouse, with its cable passing through the cable groove (1-3) at the bottom of the platform (1); Step 4: By operating the zero-field atomic magnetometer software outside the shielding device, the test of the mouse's brain magnetic signals and heart magnetic signals can be completed.

5. The testing method according to claim 4, wherein: To ensure the accuracy of the test results, the test process from step 1 to step 4 is carried out in a zero-magnetic environment of a magnetic shielding room or a magnetic shielding barrel.

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