Food composition with effects of relieving pressure and helping sleep as well as preparation method and application of food composition

Abstract

The invention discloses a food composition with effects of relieving pressure and helping sleep as well as a preparation method and application of the food composition, and belongs to the technical field of compositions. The traditional Chinese medicine composition comprises the following raw materials in parts by weight: 8-12 parts of balm, 6-10 parts of gastrodia elata powder, 6-10 parts of lucid ganoderma powder, 2-5 parts of eucommia male pollen, 1-3 parts of spina date seed powder, 1-3 parts of poria cocos powder and 6-10 parts of gamma-aminobutyric acid. According to the traditional Chinese medicine composition, aiming at insomnia syndromes in traditional Chinese medicine, through mutual cooperation of multiple targets, body functions are adjusted from multiple aspects, insomnia disorders caused by malnutrition, unease, liver depression, qi stagnation and the like are improved, meanwhile, the traditional Chinese medicine composition has a good improvement effect on sleep disorders caused by imbalance of neurotransmitters and sleep problems caused by pressure, and compared with the prior art, the traditional Chinese medicine composition has the advantages that the traditional Chinese medicine composition is worthy of popularization and application. The traditional Chinese medicine composition comprehensively improves the insomnia symptom through multiple mechanisms, and has the advantages of safety, no side effect, reliable curative effect, wide application range and the like.

Description

Technical Field

[0001] The present invention belongs to the technical field of compositions, and relates to a food composition with the efficacy of relieving stress and promoting sleep, its preparation method and application. Background Art

[0002] Insomnia is a common sleep disorder, manifested as symptoms such as difficulty falling asleep, difficulty maintaining sleep, early awakening, or poor sleep quality. It not only affects an individual's daily life and work efficiency but may also lead to a series of physical and mental health problems. The harms of insomnia to human health include mental health problems (such as anxiety, depression, etc.), physical health problems (such as chronic diseases like hypertension, heart disease, diabetes, etc.), cognitive function disorders (such as inattention, memory loss, slow reaction, etc.), and a decline in quality of life (such as reduced efficiency in daily work and study, and an increase in safety hazards in life).

[0003] In traditional Chinese medicine, insomnia is called "insomnia", "inability to sleep", "inability to lie down", or "inability to close eyes", usually caused by restlessness of the mind (hyperactivity of heart fire disturbing the mind and making it difficult to fall asleep), disharmony between the spleen and stomach (improper diet causing discomfort in the spleen and stomach and affecting sleep), exuberant liver fire (emotional distress leading to stagnation of liver qi and transforming into fire disturbing the heart), hyperactivity of fire due to yin deficiency (insufficient yin blood with internal disturbance of deficiency fire and restlessness of the mind), and insecurity of the kidney qi (insufficient kidney essence with disharmony between water and fire and disharmony between the heart and kidney).

[0004] Currently, there are many drugs and health products for treating insomnia. For example, Xu Feifei et al. (Study on the improvement effect and mechanism of the water extract of Semen Ziziphi Spinosae - Poria - Codonopsis pilosula on the sleep of mice [J], Science and Technology of Food Industry, Vol. 41, No. 11 at the end of 2021, 300 - 308) found that the water extract of Semen Ziziphi Spinosae - Poria - Codonopsis pilosula can significantly reduce the sleep latency of mice, prolong the sleep time, and reduce the spontaneous activity ability of insomnia mice, having a certain improvement effect on sleep. CN108543020A discloses a pharmaceutical health preparation for improving sleep, which uses water forget - me - not, inulin, D - mannitol, lotus seeds, rose flowers, Semen Ziziphi Spinosae, Gastrodia elata, American ginseng, Ganoderma lucidum, saffron, γ - aminobutyric acid, phosphatidylserine, taurine, calcium glycinate as raw materials for composition, and is prepared into a clinically acceptable pharmaceutical preparation according to the pharmaceutical process. In this invention, water forget - me - not and inulin are used as the monarch drugs, which play a major therapeutic role in treating the main symptoms of improving sleep; D - mannitol, lotus seeds, rose flowers, Semen Ziziphi Spinosae, Gastrodia elata are used as the ministerial drugs, assisting water forget - me - not and inulin to treat the concurrent symptoms of improving sleep; American ginseng, Ganoderma lucidum, saffron are used as the adjuvant drugs to assist the monarch and ministerial drugs to strengthen the therapeutic effect; γ - aminobutyric acid, phosphatidylserine, taurine, calcium glycinate are used as the guiding drugs, which can harmonize all the drugs in the formula, that is, drugs with the function of harmonizing various drugs. The above components are formulated in a certain proportion, so that the effects of each component produce synergy, thus achieving the effect of improving sleep.

[0005] Modern pharmacological studies have shown that the imbalance of neurotransmitters such as γ-aminobutyric acid (GABA), serotonin (5-HT), and norepinephrine (NE) can lead to sleep disorders. In addition, psychological factors such as stress and anxiety, as well as environmental factors such as noise, light, and temperature, can also affect sleep quality. Although current drugs and health products for treating insomnia have a certain improvement effect on sleep, there is still room for improvement in their effectiveness, and they have a relatively poor improvement effect on sleep disorders caused by the imbalance of neurotransmitters and sleep problems caused by stress. Therefore, it is particularly important to develop a safe, effective, wide-ranging, and cost-effective food composition for improving sleep. Summary of the Invention

[0006] The object of the present invention is to provide a food composition with the functions of relieving stress and assisting sleep, as well as its preparation method and application. Aiming at the traditional Chinese medicine insomnia syndromes, the present invention regulates the body functions from multiple aspects through the synergistic effect of multiple targets, improves insomnia disorders caused by the loss of nourishment of the mind, restlessness of the mind, and stagnation of liver qi. At the same time, it has a good improvement effect on sleep disorders caused by the imbalance of neurotransmitters and sleep problems caused by stress.

[0007] To achieve the above object, the present invention adopts the following technical solutions:

[0008] In the first aspect, the present invention provides a food composition with the functions of relieving stress and assisting sleep, which comprises the following raw materials by weight: 8-12 parts of Melissa officinalis L., 6-10 parts of Gastrodia elata Blume powder, 6-10 parts of Ganoderma lucidum powder, 2-5 parts of Eucommia ulmoides Oliver male flower powder, 1-3 parts of Semen Ziziphi Spinosae powder, and 1-3 parts of Poria cocos Wolf powder.

[0009] The present invention uses Semen Ziziphi Spinosae and Ganoderma lucidum as the monarch drugs. Semen Ziziphi Spinosae is mild in nature, sweet and sour in taste, enters the heart and liver meridians, can nourish the blood and tonify the liver, and calm the mind and soothe the nerves; Ganoderma lucidum strengthens the healthy qi and consolidates the body, enhancing the ability to resist exogenous pathogens, and Ganoderma lucidum can also tonify qi and calm the mind. The two together serve as the monarch drugs, synergistically strengthening the sedative effect, stabilizing the mind, and deepening sleep. Gastrodia elata Blume and Melissa officinalis L. are the ministerial drugs. Gastrodia elata Blume can calm the liver and extinguish wind, relieve the headache caused by stress; Melissa officinalis L. soothes the liver and relieves depression, relieving the stagnation of liver qi caused by emotional fluctuations. The two cooperate to soothe the physical and mental tension in all aspects. Eucommia ulmoides Oliver male flower and Poria cocos Wolf are the assistant drugs. Eucommia ulmoides Oliver male flower warms the kidney yang, relieves kidney deficiency fatigue, and assists in improving sleep quality; Poria cocos Wolf promotes diuresis and percolates dampness, restores the transportation and transformation of the spleen and stomach, and can also strengthen the spleen and calm the mind, helping to soothe the nerves and assist sleep, optimizing the sleep environment in all aspects.

[0010] The effects of each ingredient on insomnia in modern pharmacology are as follows: Semen Ziziphi Spinosae contains ingredients such as flavonoids and saponins, and has the effects of sedation, anti-anxiety, and promoting sleep. Modern pharmacological studies have shown that jujuboside A, B, and spinosin can up-regulate GABA and 5-HT, and down-regulate glutamate and dopamine, effectively improving sleep conditions. Ganoderma lucidum contains active ingredients such as triterpenoids and polysaccharides, and has the effects of sedation, anti-oxidation, and immune regulation. Research has shown that ganoderma polysaccharides can improve sleep conditions by regulating the nervous system and affecting the secretion of melatonin by the pineal gland. In addition, administration of ganoderma triterpenoids can significantly reduce the levels of inflammatory factors such as IL-1β, IL-6, and TNF-α in the periphery and brain of mice, and reduce anxiety and depressive-like behaviors in maternally separated mice. The active ingredients in Gastrodia elata, such as gastrodin, p-hydroxybenzyl alcohol, and p-hydroxybenzaldehyde, have sedative, anti-anxiety, and anti-depressive effects. Gastrodin can improve sleep disorders and cognitive dysfunction caused by rapid eye movement sleep deprivation by inhibiting TLR4 / NF-κB and activating the Wnt / β-catenin signaling pathway. Poria cocos contains active ingredients such as polysaccharides and triterpenoids, and has the effects of strengthening the spleen and promoting diuresis, and calming the mind. Research has shown that pachymic acid can prolong the sleep time of mice and reduce their sleep latency by increasing the protein levels of glutamate decarboxylase (GAD) 65 / 67, increasing the levels of GABAA α and β subunits, and decreasing the level of γ subunit.

[0011] Preferably, the weight ratio of the male flowers of Eucommia ulmoides and the powder of Poria cocos is 1-3:1-2; more preferably 1-3:1; even more preferably 2:1.

[0012] Preferably, the weight ratio of Melissa officinalis and the powder of Ganoderma lucidum is 4-6:4-5; more preferably 4-6:4; even more preferably 5:4.

[0013] Preferably, the weight ratio of Melissa officinalis and the powder of Gastrodia elata is 4-6:4-5; more preferably 4-6:4; even more preferably 5:4.

[0014] In some specific embodiments, the food composition comprises the following raw materials by weight: 8-12 parts of Melissa officinalis, 6-10 parts of the powder of Gastrodia elata, 6-10 parts of the powder of Ganoderma lucidum, 2-5 parts of the pollen of male flowers of Eucommia ulmoides, 1-3 parts of the powder of Semen Ziziphi Spinosae, and 1-3 parts of the powder of Poria cocos;

[0015] Among them, the weight ratio of the pollen of male flowers of Eucommia ulmoides and the powder of Poria cocos is 1-3:1, the weight ratio of Melissa officinalis and the powder of Ganoderma lucidum is 4-6:4, and the weight ratio of Melissa officinalis and the powder of Gastrodia elata is 4-6:4.

[0016] In some specific embodiments, the food composition further comprises γ-aminobutyric acid.

[0017] In the present invention, γ-aminobutyric acid is a new resource food, which can directly act on the central nervous system and has the functions of sedation, anti-anxiety and promoting sleep.

[0018] Preferably, the dosage of γ-aminobutyric acid in the food composition is 6-10 parts; more preferably 8 parts.

[0019] In some specific embodiments, the food composition comprises the following raw materials by weight: Melissa officinalis 8-12 parts, Gastrodia elata powder 6-10 parts, Ganoderma lucidum powder 6-10 parts, Eucommia ulmoides male flower pollen 2-5 parts, Ziziphus jujuba seed powder 1-3 parts, Poria cocos powder 1-3 parts and γ-aminobutyric acid 6-10 parts;

[0020] Among them, the weight ratio of Eucommia ulmoides male flower to Poria cocos powder is 2:1, the weight ratio of Melissa officinalis to Ganoderma lucidum powder is 5:4, and the weight ratio of Melissa officinalis to Gastrodia elata powder is 5:4.

[0021] In some specific embodiments, the food composition comprises the following raw materials by weight: Melissa officinalis 10 parts, Gastrodia elata powder 8 parts, Ganoderma lucidum powder 8 parts, Eucommia ulmoides male flower pollen 4 parts, Ziziphus jujuba seed powder 2 parts, Poria cocos powder 2 parts and γ-aminobutyric acid 8 parts.

[0022] In a second aspect, the present invention provides a preparation method of the above food composition, which includes the step of mixing the formula amounts of Melissa officinalis, Gastrodia elata powder, Ganoderma lucidum powder, γ-aminobutyric acid, Eucommia ulmoides male flower pollen, Ziziphus jujuba seed powder and Poria cocos powder.

[0023] In a third aspect, the present invention provides the application of the above food composition in the preparation of a neurotransmitter regulator.

[0024] In some embodiments, the neurotransmitter regulator can increase the content of γ-aminobutyric acid and / or 5-hydroxyindoleacetic acid.

[0025] In some embodiments, the neurotransmitter regulator can reduce the content of glutamate and / or glutamine.

[0026] In a fourth aspect, the present invention provides the application of the above food composition in the preparation of a product with stress relief and / or sleep aid functions.

[0027] The product includes food, health products and medicines.

[0028] The food composition in the present invention can be directly used to exert the functions of stress relief and / or sleep aid, or can be used as a raw material for preparing food, health products or medicines with stress relief and / or sleep aid functions.

[0029] In a fifth aspect, the present invention provides a product with stress relief and / or sleep aid functions, which includes the aforementioned food composition.

[0030] Preferably, the product further comprises food, health products or pharmaceutically acceptable excipients.

[0031] In some embodiments, the product is a food, health product or drug having the function of relieving stress and / or promoting sleep.

[0032] The beneficial effects of the present invention are as follows:

[0033] (1) The present invention selects natural plant components such as Melissa officinalis, Eucommia ulmoides male pollen, Gastrodia elata powder, Ganoderma lucidum powder, Ziziphus jujuba seed powder, Poria cocos powder, etc. These components have been verified by long-term traditional medicine and modern scientific research and have good safety. Different from chemical drugs, natural plant components are not prone to side effects and dependence during long-term use and are more suitable for long-term conditioning and treatment of insomnia problems.

[0034] (2) The present invention is applicable to various types of insomnia patients, including insomnia caused by stress, anxiety, emotional disorders, deficiency of both heart and spleen, deficiency of heart and gallbladder qi, stagnation of liver qi, and liver fire disturbing the heart. Through the comprehensive conditioning of various components, it can provide effective treatment for different causes of insomnia.

[0035] (3) The present invention comprehensively improves insomnia symptoms through multiple mechanisms and has many advantages such as safety without side effects, reliable curative effect, and wide application range. Detailed Embodiments

[0036] The following specific examples illustrate the embodiments of the present invention. Those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification. The present invention can also be implemented or applied through other different specific embodiments, and various details in this specification can also be modified or changed based on different viewpoints and applications without departing from the spirit of the present invention.

[0037] Before further describing the specific embodiments of the present invention, it should be understood that the protection scope of the present invention is not limited to the specific embodiments described below; it should also be understood that the terms used in the embodiments of the present invention are for describing specific embodiments and not for limiting the protection scope of the present invention.

[0038] When the embodiments give a numerical range, it should be understood that unless otherwise specified in the present invention, any value at both ends of each numerical range and any value between the two ends can be selected. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the technical field to which the present invention belongs.

[0039] The present invention does not limit the sources of the raw materials used. Unless otherwise specified, the raw materials used in the present invention are all ordinary commercially available products in the technical field. The content of rosmarinic acid in Melissa officinalis in the present invention is ≥8%, which can be purchased on the market or prepared by water extraction or alcohol extraction. The content of gastrodin in gastrodia powder is ≥0.3%, which can be purchased on the market or prepared by water extraction or alcohol extraction. The content of ganoderma polysaccharide in ganoderma powder is ≥10%, and the content of triterpenoids and sterols is ≥0.3%, which can be purchased on the market or prepared by water extraction or alcohol extraction. The content of total flavonoids in eucommia male flower pollen is ≥5%, the content of chlorogenic acid is ≥0.3%, and the content of geniposidic acid is ≥1.5%, which can be purchased on the market or prepared by water extraction or alcohol extraction. The content of jujuboside A+B in jujube seed powder is ≥0.15%, and the content of spinosin is ≥0.2%, which can be purchased on the market or prepared by water extraction or alcohol extraction. The content of pachyman in poria powder is ≥99%, and the content of γ-aminobutyric acid in γ-aminobutyric acid is ≥95%.

[0040] Example 1

[0041] A food composition for improving sleep, with the raw materials in parts by weight being: 10 parts of Melissa officinalis, 8 parts of gastrodia powder, 8 parts of ganoderma powder, 4 parts of eucommia male flower pollen, 2 parts of jujube seed powder, 2 parts of poria powder, and 8 parts of γ-aminobutyric acid.

[0042] Preparation method: Weigh the corresponding raw materials according to the formula amount and mix them.

[0043] Example 2

[0044] The difference between this example and Example 1 is that the weight ratio of the eucommia male flower pollen and the poria powder is different.

[0045] Specifically: 10 parts of Melissa officinalis, 8 parts of gastrodia powder, 8 parts of ganoderma powder, 2 parts of eucommia male flower pollen, 2 parts of jujube seed powder, 4 parts of poria powder, and 8 parts of γ-aminobutyric acid.

[0046] The preparation method is the same as that of Example 1.

[0047] Example 3

[0048] The difference between this example and Example 1 is that the weight ratio of the Melissa officinalis and the ganoderma powder is different.

[0049] Specifically: 8 parts of Melissa officinalis, 8 parts of gastrodia powder, 10 parts of ganoderma powder, 4 parts of eucommia male flower pollen, 2 parts of jujube seed powder, 2 parts of poria powder, and 8 parts of γ-aminobutyric acid.

[0050] The preparation method is the same as that of Example 1.

[0051] Example 4

[0052] The difference between this example and Example 1 is that the weight ratio of the Melissa officinalis and the gastrodia powder is different.

[0053] Specifically: 8 parts of Melissa officinalis, 10 parts of gastrodia powder, 8 parts of ganoderma powder, 4 parts of eucommia male flower pollen, 2 parts of wild jujube seed powder, 2 parts of poria powder, and 8 parts of γ-aminobutyric acid.

[0054] The preparation method is the same as that of Example 1.

[0055] Comparative Example 1

[0056] In this comparative example, the total amount of Melissa officinalis, gastrodia powder, ganoderma powder, eucommia male flower pollen, and poria powder is still 32 parts as described in Example 1. The difference is that Melissa officinalis is removed, and the amounts of gastrodia powder and ganoderma powder are increased in equal proportion.

[0057] Specifically: 13 parts of gastrodia powder, 13 parts of ganoderma powder, 4 parts of eucommia male flower pollen, 2 parts of wild jujube seed powder, 2 parts of poria powder, and 8 parts of γ-aminobutyric acid.

[0058] The preparation method is the same as that of Example 1.

[0059] Comparative Example 2

[0060] In this comparative example, the total amount of Melissa officinalis, gastrodia powder, ganoderma powder, eucommia male flower pollen, and poria powder is still 32 parts as described in Example 1. The difference is that the weight ratio of Melissa officinalis to ganoderma powder and the weight ratio of eucommia male flower to poria powder are changed.

[0061] Specifically: 6 parts of Melissa officinalis, 8 parts of gastrodia powder, 12 parts of ganoderma powder, 5 parts of eucommia male flower pollen, 2 parts of wild jujube seed powder, 1 part of poria powder, and 8 parts of γ-aminobutyric acid.

[0062] The preparation method is the same as that of Example 1.

[0063] Comparative Example 3

[0064] In this comparative example, the total amount of Melissa officinalis, gastrodia powder, ganoderma powder, eucommia male flower pollen, and poria powder is still 32 parts as described in Example 1. The difference is that the dosage relationship of the five components is different.

[0065] Specifically: 15 parts of Melissa officinalis, 2 parts of gastrodia powder, 5 parts of ganoderma powder, 6 parts of eucommia male flower pollen, 2 parts of wild jujube seed powder, 4 parts of poria powder, and 8 parts of γ-aminobutyric acid.

[0066] The preparation method is the same as that of Example 1.

[0067] Experimental Example 1: Efficacy Verification

[0068] 1. Experimental Animals

[0069] ICR male mice (SCXK(Shanghai) 2023-0004), weighing about 25 g, were purchased from Shanghai Jiesijie Laboratory Animal Co., Ltd. and raised in a SPF-class animal house (number SCXK(Shanghai) 2023-0004). The quarantine certificate number of the mice was 3100421528, and there were 108 mice in total.

[0070] 2. Experimental methods

[0071] The mice were randomly divided into 9 groups, with 12 mice in each group, namely the blank control group with normal diet, the positive control group with melatonin added, Example 1 group, Example 2 group, Example 3 group, Example 4 group, Comparative Example 1 group, Comparative Example 2 group, and Comparative Example 3 group. The mice in the blank control group were gavaged with double-distilled water, the mice in the positive control group were gavaged with melatonin (at a dose of 0.5 mg / kg), and the mice in the experimental groups were gavaged with the corresponding food composition according to their body weight (at a dose of 230 mg / kg). The following two experiments were carried out respectively: (1) Sleep induction experiment: 30 minutes after gavaging the test substance, sodium pentobarbital (45 mg / kg) was injected intraperitoneally, and the sleep onset time of each group of mice was recorded. (2) Righting experiment: The time from supine to righting of each mouse was evaluated, that is, the sleep time, to reflect the sleep depth.

[0072] The results are shown in Table 1.

[0073] Table 1 Effects of food compositions in different groups on the sleep time induced by sodium pentobarbital

[0074]

[0075]

[0076] Note: Compared with the blank control group, *p < 0.05 indicates a significant difference; **p < 0.01 indicates a highly significant difference.

[0077] Experimental results: Compared with the blank control group, the positive control group and Example 1-4 groups could significantly shorten the sleep onset time of mice (P < 0.01) and significantly prolong the sleep time of mice (P < 0.01). Among them, the sleep extension rate of Example 1 group was 201.7%, which was significantly higher than that of Example 2-4 groups, indicating that the food compositions provided by Example 1-4 of the present invention could shorten the sleep onset time and prolong the sleep time, and the effect of Example 1 was the best. Compared with the blank control group, there was no significant change in the sleep time of mice in Comparative Example 1-3 groups (P > 0.05), indicating that the food compositions provided by Comparative Example 1-3 did not have the effect of prolonging the sleep time.

[0078] Experimental Example 2: Study on the sleep improvement function test

[0079] 1. Experimental materials

[0080] Glutamate colorimetric assay kit (No. WP08RX666132), Glutamine colorimetric assay kit (No. WV10NNHN2444), γ-Aminobutyric acid colorimetric assay kit (No. VVP10R0BB9885), 5-Hydroxytryptamine enzyme-linked immunosorbent assay kit (No. WV06DNHB6189), 5-Hydroxyindoleacetic acid enzyme-linked immunosorbent assay kit (No. WV056F484625) were purchased from Wuhan Elitre Biotech Co., Ltd.

[0081] 2. Experimental animals

[0082] ICR male mice (SCXK(Shanghai)2023-0004), weighing about 25 g, were purchased from Shanghai Jiesijie Experimental Animal Co., Ltd. and raised in a SPF-class animal room (No. SCXK(Shanghai)2023-0004). The quarantine certificate number of the mice was 3100421528. There were 200 mice in total, divided into 20 groups for breeding, with 10 mice in each group.

[0083] 3. Experimental instruments

[0084] TGL-18M high-speed refrigerated centrifuge (Shanghai LUXIANGYI Centrifuge Instrument Co., Ltd.), DK-8D electrothermal constant temperature water bath (Shanghai Jinghong Experimental Equipment Co., Ltd.), AmiconUltra 10kD and 50kD ultrafiltration tubes, SuPerMax 2700MF multi-functional microplate reader (Shanghai Flash Spectrum Biotech Co., Ltd.).

[0085] 4. Experimental methods

[0086] 4.1 Direct sleep experiment

[0087] Male ICR mice were used in the experiment. Five groups were set up, namely a blank control group with normal diet, a positive control group with melatonin added, a low-dose group (5 times the human recommended dose) and a high-dose group (20 times the human recommended dose) of Example 4, and a control group of Comparative Example 1. The specific gavage doses are shown in Table 2.

[0088] Table 2 Gavage doses of each group

[0089]

[0090] Ten mice in each group were given the test substance by gavage. After that, the sleep effect was observed (sleep was judged by the disappearance of the righting reflex), the number of sleeping animals and the sleep time were recorded, and glutamate, glutamine, γ-aminobutyric acid, 5-hydroxytryptamine, and 5-hydroxyindoleacetic acid in the mouse brain tissue were measured.

[0091] The results are shown in Table 3 and Table 4.

[0092] Table 3 Number of sleeping mice in the direct sleep experiment (n = 10)

[0093]

[0094] Table 4 Content of neurotransmitters in the brain tissue of mice in the direct sleep experiment

[0095]

[0096] Experimental results: After gavage in each group of mice, the disappearance of the righting reflex did not occur within 60 min, as shown in Table 3. As shown in Table 4, compared with the control group, the contents of glutamate, glutamine, γ-aminobutyric acid, 5-hydroxytryptamine, and 5-hydroxyindoleacetic acid in the brain tissue of each group of mice fluctuated, but these changes did not reach a significant level. Therefore, the food compositions of Example 4 and Comparative Example 1 do not have a direct sleep-inducing effect, and the results indicate that the food composition of the present invention does not have a mechanism of action similar to that of sleeping pills.

[0097] 4.2 Experiment on prolonging the sleeping time of sodium pentobarbital

[0098] Male ICR mice were used. The experiment was set up with 5 groups, namely a blank control group with normal diet, a positive control group added with melatonin, and three dose groups of low (5 times the human recommended dose), high (20 times the human recommended dose) of Experimental Example 4, and high dose of Comparative Example 1. The specific gavage doses are shown in Table 2 above. There were 10 mice in each group. After gavage with the test substance for 4 weeks, 30 min after the last administration of the test substance, sodium pentobarbital (45 mg / kg) at a dose that could make 100% of the mice fall asleep was injected intraperitoneally, and the sleeping time of the mice was recorded. The contents of glutamate, glutamine, γ-aminobutyric acid, 5-hydroxytryptamine, and 5-hydroxyindoleacetic acid in the brain tissue of the mice were measured.

[0099] The results are shown in Tables 5 and 6.

[0100] Table 5 Sleeping duration of mice in the experiment on prolonging the sleeping time of sodium pentobarbital( n = 10)

[0101]

[0102] Note: Compared with the blank control group, *p < 0.05 indicates a significant difference; **p < 0.01 indicates a highly significant difference.

[0103] Table 6 Content of neurotransmitters in the brain tissue of mice in the experiment on prolonging the sleeping time of sodium pentobarbital( n = 10)

[0104]

[0105] Note: Compared with the blank control group, *p < 0.05 indicates a significant difference; **p < 0.01 indicates a highly significant difference.

[0106] Experimental results: As shown in Table 5, compared with the blank control group, the sleep duration of each group was significantly increased. The sleep durations of the low- and high-dose groups of Example 4 were increased by 76.1% and 128.3% respectively, and both were significant, indicating that the composition of the Example 4 group was significant in terms of prolonging sleep time, and the experimental results were positive. As shown in Table 6, compared with the blank control group, the contents of glutamate and glutamine in the positive control group were significantly decreased. The low- and high-dose groups of Example 4 could significantly increase the content of 5-hydroxyindoleacetic acid, and the high-dose group of Example 4 could also significantly increase the content of γ-aminobutyric acid.

[0107] 4.3 Hypnotic experiment with subthreshold dose of sodium pentobarbital

[0108] Male ICR mice were used. The experiment was set up with 5 groups, namely the blank control group with normal diet, the positive control group with melatonin added, the low- (5 times the human recommended dose) and high- (20 times the human recommended dose) dose groups of Example 4, and the group of Comparative Example 1. The specific gavage doses are shown in Table 2 above. There were 10 mice in each group. After gavaging the test substances for 4 weeks, 30 minutes after the last administration of the test substance, the maximum subthreshold dose of sodium pentobarbital (30 mg / kg) that did not cause the disappearance of the righting reflex was intraperitoneally injected, and the number of mice that fell asleep within 30 minutes was recorded. The contents of glutamate, glutamine, γ-aminobutyric acid, 5-hydroxytryptamine, and 5-hydroxyindoleacetic acid in the mouse brain tissues were measured.

[0109] The results are shown in Tables 7 and 8.

[0110] Table 7 Number of mice falling asleep in the hypnotic experiment with subthreshold dose of sodium pentobarbital (n = 10)

[0111]

[0112] Note: Compared with the blank control group, *p < 0.05 indicates significant difference.

[0113] Table 8 Contents of neurotransmitters in the brain tissues of mice in the hypnotic experiment with subthreshold dose of sodium pentobarbital n = 10

[0114]

[0115] Note: Compared with the blank control group, *p < 0.05 indicates significant difference; **p < 0.01 indicates extremely significant difference.

[0116] Experimental results: The number of mice that fell asleep within 30 minutes in the subthreshold dose hypnosis experiment with sodium pentobarbital is shown in Table 7. The number of mice that fell asleep in the positive control group, the low and high dose groups of Example 4 was 6 each, showing a significant sleep improvement effect. The number of mice that fell asleep in the Comparative Example 1 group was 4, without a sleep improvement effect. As shown in Table 8, compared with the blank control group, the positive control group could significantly increase the contents of γ-aminobutyric acid and 5-hydroxyindoleacetic acid. The low and high dose groups of Example 4 could significantly reduce the contents of glutamic acid and glutamine and significantly increase the contents of γ-aminobutyric acid and 5-hydroxyindoleacetic acid.

[0117] 4.4 Sleep latency experiment with sodium barbital

[0118] Male ICR mice were used. The experiment was set up with 5 groups, namely the blank control group with normal diet, the positive control group with melatonin added, the low (5 times the human recommended dose) and high (20 times the human recommended dose) dose groups of Example 4, and the Comparative Example 1 group. The specific gavage doses are shown in Table 2 above. There were 10 mice in each group. After gavaging the test substances for 4 weeks, 30 minutes after the last administration of the test substances, sodium barbital (250 mg / kg) at a dose that could make 100% of the mice fall asleep was injected intraperitoneally, and the sleep latency time of each group of mice was recorded. The contents of glutamic acid, glutamine, γ-aminobutyric acid, 5-hydroxyindoleacetic acid, and 5-hydroxytryptamine in the mouse brain tissues were measured.

[0119] The results are shown in Tables 9 and 10.

[0120] Table 9 Sleep latency of mice in the sleep latency experiment with sodium barbital n = 10

[0121]

[0122] Note: Compared with the blank control group, *p < 0.05 indicates a significant difference.

[0123] Table 10 Contents of neurotransmitters in the brain tissues of mice in the sleep latency experiment with sodium barbital n = 10

[0124]

[0125] Note: Compared with the blank control group, *p < 0.05 indicates a significant difference; **p < 0.01 indicates a highly significant difference.

[0126] Experimental results: The effects of the barbiturate sodium sleep latency of mice in each experimental group are shown in Table 9. The sleep latencies of the low and high dose groups in Example 4 were significantly different compared with the blank control group, shortening by 29.1% and 31.8% respectively compared with the blank control group. The shortening of the sleep latency was significant, and the experimental results were positive. As shown in Table 10, compared with the blank control group, the positive control group could significantly reduce the contents of glutamate and glutamine and increase the content of 5-hydroxytryptamine. The low and high dose groups in Example 4 could significantly reduce the contents of glutamate and glutamine and significantly increase the contents of γ-aminobutyric acid and 5-hydroxyindoleacetic acid.

[0127] The above experimental results show that both the low and high dose groups in Example 4 significantly prolonged the pentobarbital sodium sleeping time of mice; the number of sleeping mice at the subthreshold dose of pentobarbital sodium in the low and high dose groups in Example 4 increased significantly; both the low and high dose groups in Example 4 significantly shortened the barbiturate sodium sleep latency; according to the "Methods for Functional Testing and Evaluation of Health Foods (2023 Edition)", that is, any two of the three experiments of prolonging the pentobarbital sodium sleeping time experiment, pentobarbital sodium subthreshold dose hypnosis experiment, and barbiturate sodium sleep latency experiment are positive, and there is no obvious direct sleep effect, it can be determined that the composition in Example 4 has the effect of helping to improve sleep, and the composition in Comparative Example 1 has no effect on improving sleep.

[0128] In addition, the food composition in Example 4 can significantly reduce the contents of excitatory neurotransmitters glutamate and glutamine, and increase the contents of inhibitory neurotransmitters γ-aminobutyric acid and 5-hydroxyindoleacetic acid, thereby effectively regulating the excitatory and inhibitory balance of the nervous system, reducing the over-excited state of neurons caused by long-term stress, and relieving psychological stress and anxiety.

[0129] The food compositions prepared in the above Examples 1-3 of the present invention have the same or even better technical effects in improving sleep as Example 4. Details are not described herein again.

[0130] The above is a further description of the present invention in combination with specific embodiments, but these embodiments are only exemplary and do not constitute any limitation to the scope of the present invention. Those skilled in the art should understand that the details and forms of the technical solutions of the present invention can be modified or replaced without departing from the spirit and scope of the present invention, but these modifications and replacements all fall within the protection scope of the present invention.

Claims

1. A food composition with stress-relieving and sleep-aiding functions, characterized in that: The raw materials are as follows, measured by weight: 8-12 parts of lemon balm, 6-10 parts of gastrodia powder, 6-10 parts of ganoderma lucidum powder, 2-5 parts of eucommia male pollen, 1-3 parts of spiny jujube seed powder, 1-3 parts of poria powder and 6-10 parts of gamma-aminobutyric acid.

2. The food composition according to claim 1, characterized in that The weight ratio of the Eucommia male pollen to the Poria powder is 1-3:1-2; and / or the weight ratio of lemon balm to ganoderma lucidum powder is 4-6:4-5; And / or the weight ratio of lemon balm to gastrodia powder is 4-6:4-5.

3. The food composition according to claim 1, characterized in that The weight ratio of the Eucommia male pollen to Poria powder is 2:1, the weight ratio of the lemon balm to Ganoderma lucidum powder is 5:4, and the weight ratio of the lemon balm to Gastrodia elata powder is 5:

4.

4. The food composition according to claim 1, characterized in that The raw materials are comprised of the following by weight: 10 parts of lemon balm, 8 parts of gastrodia powder, 8 parts of ganoderma powder, 4 parts of eucommia male pollen, 2 parts of spiny jujube seed powder, 2 parts of tuckahoe powder and 8 parts of gamma-aminobutyric acid.

5. The method for preparing the food composition according to any one of claims 1 to 4, characterized in that: The method comprises the steps of mixing a formula amount of lemon balm, gastrodia powder, ganoderma powder, eucommia male pollen, spiny jujube seed powder, tuckahoe powder and gamma-aminobutyric acid.

6. Use of the food composition according to any one of claims 1 to 5 in the preparation of a neurotransmitter regulator.

7. The use according to claim 6, characterized in that: The neurotransmitter modulator has at least one of the following characteristics: (1) Increase the content of γ-aminobutyric acid; (2) increase the content of 5-hydroxyindoleacetic acid; (3) Reduce glutamate content; (4) Reduce glutamine content.

8. Use of the food composition according to any one of claims 1 to 5 in preparing products having the effects of relieving stress and / or promoting sleep.

9. The use according to claim 8, characterized in that: The product is food, health product or medicine.

10. A product with stress relief and / or sleep aid function, characterized in that: A food composition comprising any one of claims 1 to 5; preferably, the product further comprises an excipient acceptable in food, health care products or medicines.

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