Bee product composite preparation and low-temperature emulsification preparation method thereof

Abstract

The invention discloses a bee product compound preparation and a low-temperature emulsification preparation method thereof, and belongs to the technical field of bee product processing, and the bee product compound preparation comprises the following raw materials: royal jelly, bee pollen and honey. The preparation method comprises the following steps: pretreating the raw materials; adding the pretreated raw materials into a low-temperature emulsifying machine, and stirring at a low speed; performing low-temperature emulsification at the temperature lower than 50 DEG C, and finally performing vacuum defoaming and ultraviolet sterilization curing to obtain the bee product composite preparation. The bee product composite preparation is a uniform and fine emulsion, is uniform in color and luster and has the natural color of honey and the unique fragrance of royal jelly and bee pollen, and the bee pollen is subjected to ultramicro wall breaking treatment, so that bioactive substances in the bee pollen can be fully released, and the bioactive substances and other components in the bee product composite preparation can better play a synergistic effect, and the bee product composite preparation has a good application prospect. The bioavailability is improved; and a low-temperature emulsification method is adopted, so that heat-sensitive nutritional ingredients in the bee product can be reserved to the greatest extent, and the nutritional value and the stability of the product are improved.

Description

Technical Field

[0001] The present invention relates to the technical field of bee product processing, and particularly relates to a bee product composite preparation composed of multiple bee products and a low-temperature emulsification preparation method thereof. Background Art

[0002] Due to their rich nutritional components and diverse health care effects, bee products have attracted much attention in the fields of food, health products, and medicine. Honey is rich in various sugars, vitamins, minerals, and bioactive substances, and has functions such as moistening the lungs and relieving cough, loosening the bowels and relieving constipation, antibacterial and anti-inflammatory, etc.; propolis contains compounds such as flavonoids and terpenoids, and has effects such as antioxidant, enhancing immunity, and lowering blood lipids; royal jelly is rich in proteins, amino acids, vitamins, and bioactive factors, and can regulate endocrine, delay aging, and improve the body's resistance. Compared with single bee products, bee product compositions have many significant advantages: from a nutritional perspective, they can integrate the nutritional components of multiple bee products to achieve nutritional diversification. The rich sugars, vitamins, and minerals in honey, the flavonoids and terpenoids in propolis, and the proteins, amino acids, vitamins, and bioactive factors contained in royal jelly complement each other in the composite preparation, providing more comprehensive nutritional support for the human body. In terms of health care effects, the components of multiple bee products act synergistically, significantly enhancing the health care effect.

[0003] Existing bee product compositions generally have the following technical defects: traditional mixing processes cause the inactivation of active ingredients (such as 10-hydroxy-2-decenoic acid, enzyme substances) at high temperatures; the stability of the multiphase system is poor, and stratification / crystallization phenomena are prone to occur; the bee pollen wall-breaking treatment is insufficient, affecting the bioavailability; the residual bubbles result in uneven product texture. Summary of the Invention

[0004] The object of the present invention is to solve the problems of inactivation of active ingredients, poor stability of the multiphase system, uneven texture, and low bioavailability of existing bee product compositions, and to provide a bee product composite preparation and a low-temperature emulsification preparation method thereof. The bee product composite preparation prepared by this method can fully integrate the nutritional components of royal jelly, bee pollen, and honey, play a synergistic effect, and at the same time adopt low-temperature emulsification technology to maximize the retention of heat-sensitive nutritional components in bee products, improving the nutritional value and stability of the product.

[0005] One of the technical solutions adopted by the present invention to solve the above technical problems is to provide a bee product composite preparation, which includes the following raw materials in weight percentages: 1-3% of royal jelly, 3-5% of bee pollen, and 92-96% of honey.

[0006] Preferably, the 10-HDA content of the royal jelly is ≥1.8%. 10-HDA (10-hydroxy-2-decenoic acid) is a unique fatty acid in royal jelly and a marker component of royal jelly. Its content is stable at a certain level, indicating the purity and authenticity of royal jelly in the bee product compound preparation, ensuring reliable product quality and guaranteeing the overall quality of the bee product compound preparation.

[0007] Preferably, the bee pollen is treated by ultra-fine wall-breaking, with a wall-breaking rate ≥98% and a particle size ≤5 μm. After ultra-fine wall-breaking treatment, the particle size of the bee pollen becomes smaller and the wall-breaking rate is high. There will be no rough feeling in the bee product compound preparation, making the product taste smoother and more delicate; the bee pollen particles with a particle size ≤5 μm are more uniform and finer, and are not prone to aggregation and precipitation in the bee product compound preparation, keeping the product in a stable physical state during storage and transportation, and ensuring the uniformity and consistency of the product; the ultra-fine wall-broken bee pollen can be more evenly dispersed in the compound preparation, making the product color more uniform, the appearance more attractive, and improving the market competitiveness of the product.

[0008] Preferably, the Baumé degree of the honey is ≥42°. For honey with a Baumé degree ≥42°, the water content is usually below 19%. The lower water content can reduce the possibility of microbial growth and the risk of honey fermentation and spoilage, helping to ensure the stability and quality of the bee product compound preparation during storage and use.

[0009] The bee product compound preparation of the present invention is a uniform and delicate emulsion with a uniform color, having the natural color of honey and the unique aroma of royal jelly and bee pollen. Each component in the product is evenly dispersed and has good stability, without phase separation at a centrifugation rate of 3000 rpm / 30 min, with excellent stability, and the shelf life at room temperature is extended to 24 months. This compound preparation is rich in various nutritional components and bioactive substances in royal jelly, bee pollen, and honey, and has multiple health care effects such as enhancing immunity, antioxidation, regulating endocrine, and promoting metabolism.

[0010] To solve the above technical problems, the second technical solution adopted by the present invention is to provide a low-temperature emulsification preparation method for this bee product compound preparation, including the following steps:

[0011] (1) Raw material pretreatment: Naturally thaw the royal jelly at a low temperature of 0-5°C for pre-activation; use a three-stage gradient wall-breaking technology to perform ultra-fine wall-breaking treatment on the bee pollen until the wall-breaking rate ≥98% and the particle size ≤5 μm;

[0012] (2) Mixing: First inject 50-60% of the honey into the reaction kettle, then sequentially inject the pretreated bee pollen and royal jelly, and finally inject the remaining honey, and stir at a low speed for at least 15 min to form a primary dispersion system;

[0013] (3) Low-temperature emulsification: Using a stepwise temperature increase method, raise the temperature to 50°C, and alternately perform high-speed shear emulsification and low-speed stirring under a low vacuum. The total duration of high-speed shear emulsification and low-speed stirring is at least 30 minutes.

[0014] (4) Vacuum defoaming: Perform vacuum defoaming using a three-stage vacuum gradient defoaming treatment, and each stage of the vacuum gradient is maintained for at least 5 minutes.

[0015] (5) Aging: First, let it stand at room temperature for at least 24 hours, and then perform ultraviolet sterilization to obtain the bee product compound preparation.

[0016] Preferably, the three-stage gradient cell wall breaking technology described in step (1) means first performing ultrafine cell wall breaking to a particle size ≤ 40 μm, then cell wall breaking to a particle size ≤ 20 μm, and finally cell wall breaking to a particle size ≤ 5 μm.

[0017] Preferably, the stirring speed of the low-speed stirring described in steps (2) and (3) is 25 - 35 rpm.

[0018] Preferably, the stepwise temperature increase method described in step (3) is to first increase the temperature from room temperature to 35°C, maintain it for at least 10 minutes, then continue to increase the temperature to 45°C, maintain it for at least 10 minutes, and then continue to increase the temperature to 50°C; the low vacuum refers to a vacuum degree of -0.08 MPa; the stirring speed of the high-speed shear is 8000 rpm.

[0019] Preferably, the three-stage vacuum gradients described in step (4) are -0.05 MPa, -0.08 MPa, and -0.1 MPa vacuum degrees respectively.

[0020] Preferably, the ultraviolet wavelength of the ultraviolet sterilization described in step (5) is 254 nm, and the intensity is 30 μW / cm 2 .

[0021] Compared with the prior art, the beneficial effects of the present invention are:

[0022] 1. Protecting nutritional components: Some nutritional components in bee products, such as 10-HDA in royal jelly, various bioenzymes, enzymes and vitamins in honey, and some bioactive substances in bee pollen, are sensitive to temperature. Low-temperature emulsification can avoid the denaturation and inactivation of these thermosensitive substances due to high temperature during the preparation process, and retain their biological activity and nutritional value to the greatest extent; the lower temperature can reduce the possibility of chemical reactions between nutritional components in bee products, reduce the loss of nutritional components caused by oxidation, hydrolysis and other reactions, and make the nutrition of the final product more abundant and complete. After detection, the retention rate of 10-HDA in royal jelly ≥ 95%; the SOD enzyme activity ≥ 89%.

[0023] 2. Improve product stability: Through the high-shear function and circulating emulsification method of the low-temperature emulsifier, the uniform emulsification of royal jelly, bee pollen and honey is achieved, resulting in good product stability, less prone to layering and precipitation. The bee product compound preparation maintains good physical stability during storage and transportation, with a more uniform appearance and texture.

[0024] 3. Improve bioavailability: The three-stage gradient wall-breaking technology is used for ultrafine wall-breaking treatment until the wall-breaking rate ≥ 98% and the particle size ≤ 5μm. The bioactive substances in bee pollen are fully released after ultrafine wall-breaking and can better play a synergistic role with other components in the bee product compound preparation, such as royal jelly and honey, to improve its bioavailability. Detailed implementation manners

[0025] The technical solutions of the present invention will be clearly and completely described below in conjunction with the embodiments. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the scope of protection of the present invention.

[0026] Embodiment 1

[0027] This embodiment provides a bee product compound preparation, including the following raw materials: 2 kg of royal jelly with a 10-HDA content of 2.0%, 4 kg of ultrafine wall-broken bee pollen with a wall-breaking rate of 98.6% and a particle size of 4μm, and 94 kg of Sophora japonica honey with a Baume degree ≥ 42° and a glucose content ≤ 28%.

[0028] The low-temperature emulsification preparation method includes the following steps:

[0029] (1) Raw material pretreatment: The royal jelly stored at -20°C is naturally thawed at 5°C for 24 h for pre-activation treatment; the bee pollen is first ultrafine wall-broken to a particle size of 35μm, then wall-broken to a particle size of 20μm, and finally wall-broken to a particle size of 4μm, with a wall-breaking rate of 98.6%.

[0030] (2) Mixing: First, 60% of the Sophora japonica honey is injected into the reaction kettle, then the pretreated bee pollen and royal jelly are injected in sequence, and finally the remaining Sophora japonica honey is injected, and stirred at a low speed of 30 rpm for 20 min to form a primary dispersion system.

[0031] (3) Low-temperature emulsification: a step-by-step heating method was used, firstly the temperature was raised from room temperature to 35°C, maintained for 10 min, then raised to 45°C, maintained for 10 min, and then further raised to 50°C, and the temperature was kept constant at 50°C. High-speed shearing and low-speed stirring emulsification were performed alternately under a low vacuum degree of -0.08 MPa. The total duration of high-speed shearing and low-speed stirring was 35 min, wherein the stirring speed of high-speed shearing was 8000 rpm, and the stirring speed of low-speed stirring was 30 rpm;

[0032] (4) Vacuum defoaming: first adjust the vacuum degree to -0.05 MPa, maintain for 5 min, then adjust to -0.08 MPa, maintain for 5 min, and finally adjust to -0.1 MPa, also maintain for 5 min for vacuum defoaming;

[0033] (5) Ripening: First, place at room temperature for 24 hours, then place under ultraviolet light at a wavelength of 254 nm and an intensity of 30 μW / cm 2 UV sterilization is performed under the conditions of to obtain a bee product composite preparation;

[0034] (6) Filling and packaging: The prepared bee product compound preparation is transported to the filling machine through a sterile pipeline, filled in a sterile environment, and sealed and packaged.

[0035] The bee product composite preparation product of the present embodiment presents a uniform and delicate milky state, light yellow and translucent in color, with a unique and rich aroma of acacia honey mixed with other bee products, and a sweet and soft taste. After testing, the composite preparation has no phase separation at a centrifugal rate of 3000rpm / 30min, excellent stability, and a shelf life of 24 months at room temperature. The product is analyzed for nutritional ingredients, and the results show that the product not only contains rich acacia honey nutrients, but also fully retains the flavonoids in propolis, terpenoid compounds, and proteins, amino acids, vitamins and bioactive factors in royal jelly, and the bioactive factors maintain high activity, 10-HDA retention rate is 96.7%, SOD enzyme activity is maintained at 89.3%, and in vitro simulated digestion experiments show that total phenol release increases by 42%.

[0036] Example 2

[0037] The present embodiment provides a bee product composite preparation, comprising the following raw materials: 1 kg of royal jelly with a 10-HDA content of 1.9%, 3 kg of ultrafine wall-broken bee pollen with a wall-breaking rate of 98.3% and a particle size of 5 μm, and 96 kg of linden honey with a Baume degree of 43°.

[0038] The low-temperature emulsification preparation method comprises the following steps:

[0039] (1) Raw material pretreatment: The royal jelly stored frozen at -18°C is thawed at a constant temperature of 3°C for 36 hours for pre-activation treatment; the bee pollen is first ultrasonically broken to a particle size of 40 μm, then to 25 μm, and finally to 5 μm, with a breakage rate of 98.3%.

[0040] (2) Mixing: First, 50% of linden honey is injected into the reaction kettle, then the pretreated bee pollen and royal jelly are injected in sequence, and finally the remaining linden honey is injected. It is stirred at a low speed of 35 rpm for 15 minutes to form a primary dispersion system.

[0041] (3) Low-temperature emulsification: Using a stepwise temperature increase method, first raise the temperature from room temperature to 35°C and maintain it for 10 minutes, then continue to raise the temperature to 45°C and maintain it for 10 minutes, and then continue to raise the temperature to 50°C and keep the temperature at 50°C unchanged. High-speed shear emulsification and low-speed stirring are alternately carried out under a low vacuum of -0.08 MPa. The total duration of high-speed shear emulsification and low-speed stirring is 35 minutes, where the stirring speed of high-speed shear emulsification is 8000 rpm and the stirring speed of low-speed stirring is 35 rpm.

[0042] (4) Vacuum defoaming: First, the vacuum is adjusted to -0.05 MPa and maintained for 10 minutes, then adjusted to -0.08 MPa and maintained for 10 minutes, and finally adjusted to -0.1 MPa and also maintained for 10 minutes for vacuum defoaming.

[0043] (5) Aging: First, it is left to stand at room temperature for 24 hours, and then ultraviolet sterilization is carried out under the conditions of an ultraviolet wavelength of 254 nm and an intensity of 30 μW / cm 2 to obtain the compound preparation of bee products.

[0044] (6) Filling and packaging: The prepared compound preparation of bee products is transported to the filling machine through a sterile pipeline, and filling is carried out in a sterile environment and sealed for packaging.

[0045] The bee product composite preparation product of the present embodiment presents uniform, delicate milky texture, and the color is light yellow and glossy, which is the unique color after linden honey is mixed with other bee products.The product has a unique and charming fragrance, which combines the flower fragrance of linden honey, the special smell of propolis and the breath of royal jelly, and the fragrance is rich in layers, and the taste is sweet and moderate, soft and smooth.After testing, the composite preparation has no phase separation at a centrifugal rate of 3000rpm / 30min, and the stability is excellent, and the shelf life is extended to 24 months at room temperature.The product is analyzed for nutritional ingredients, and the result shows that the product not only contains rich linden honey nutrients, but also fully retains the flavonoids in propolis, terpenoid compounds and royal jelly proteins, amino acids, vitamins and bioactive factors, and the bioactive factors maintain high activity, 10-HDA retention rate is 95.8%, SOD enzyme activity is maintained at 89.6%, and in vitro simulated digestion experiments show that total phenol release increases by 42.3%.

[0046] Example 3

[0047] This embodiment provides a bee product composite preparation, comprising the following raw materials: 3 kg of royal jelly with a 10-HDA content of 1.9%, 5 kg of ultra-fine wall-broken bee pollen with a wall-breaking rate of 98.8% and a particle size of 5 μm, and 92 kg of vitex honey with a Baume degree of 42°.

[0048] The low-temperature emulsification preparation method comprises the following steps:

[0049] (1) Raw material pretreatment: Royal jelly stored at -22°C was thawed at 0°C for 48 hours for pre-activation; Bee pollen was first ultra-micronized to a particle size of 40 μm, then to a particle size of 25 μm, and finally to a particle size of 5 μm, with a breaking rate of 98.8%;

[0050] (2) Mixing: First, inject 55% of the acacia honey into the reactor, then inject the pretreated bee pollen and royal jelly in sequence, and finally inject the remaining honey, and stir at a low speed of 25 rpm for 20 minutes to form a primary dispersion system;

[0051] (3) Low-temperature emulsification: a step-by-step heating method was used, firstly the temperature was raised from room temperature to 35°C, maintained for 10 min, then raised to 45°C, maintained for 10 min, and then further raised to 50°C. The temperature was kept constant at 50°C, and high-speed shear emulsification and low-speed stirring were performed alternately under a low vacuum degree of -0.08 MPa. The total duration of high-speed shear emulsification and low-speed stirring was 40 min, wherein the stirring speed of high-speed shear emulsification was 8000 rpm, and the stirring speed of low-speed stirring was 25 rpm;

[0052] (4) Vacuum defoaming: First, adjust the vacuum degree to -0.05 MPa and maintain it for 8 min, then adjust it to -0.08 MPa and maintain it for 8 min, and finally adjust it to -0.1 MPa and also maintain it for 8 min for vacuum defoaming;

[0053] (5) Aging: First, let it stand at room temperature for 48 h, and then perform ultraviolet sterilization under the conditions of an ultraviolet wavelength of 254 nm and an intensity of 30 μW / cm 2 to obtain the bee product compound preparation;

[0054] (6) Filling and packaging: Transport the prepared bee product compound preparation to the filling machine through a sterile pipeline, and perform filling and sealed packaging in a sterile environment.

[0055] The bee product compound preparation product of this example is a uniform emulsion, with a light amber color, having the unique aroma of Vitex honey and the odors of royal jelly and bee pollen. After testing, the compound preparation has no phase separation at a centrifugation rate of 3000 rpm / 30 min, and has excellent stability. The shelf life at room temperature is extended to 24 months. Analyze the nutritional components of the product. The results show that the product not only contains rich nutritional components of Vitex honey, but also fully retains the flavonoids, terpenoids in propolis, as well as proteins, amino acids, vitamins and bioactive factors in royal jelly, and the bioactive factors maintain a high activity. The retention rate of 10-HDA is 96.3%, and the SOD enzyme activity is maintained at 90.2%. The in vitro simulated digestion experiment shows that the total phenol release amount increases by 43.1%.

[0056] The above is only the best implementation mode of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the principle of the present invention, several deformations or equivalent replacements can be made to the technical solution of the present invention, and the same technical effects can also be achieved, which should also be regarded as belonging to the protection scope of the present invention.

Claims

1. A bee product composite preparation, characterized in that: The invention comprises the following raw materials in percentage by weight: 1-3% royal jelly, 3-5% bee pollen and 92-96% honey.

2. The bee product composite preparation according to claim 1, characterized in that: The 10-HDA content of the royal jelly is ≥1.8%.

3. The bee product composite preparation according to claim 2, characterized in that: The bee pollen is ultra-fine wall-broken bee pollen with a wall-breaking rate of ≥98% and a particle size of ≤5μm.

4. The bee product composite preparation according to claim 3, characterized in that: The Baume degree of the honey is ≥42°.

5. The low-temperature emulsification preparation method of the bee product composite preparation according to any one of claims 1 to 4, characterized in that: The following steps are involved: (1) Raw material pretreatment: the royal jelly is naturally thawed at a low temperature of 0 to 5°C for pre-activation; the bee pollen is ultra-micro-broken using a three-stage gradient wall-breaking technology to a wall-breaking rate of ≥98% and a particle size of ≤5 μm; (2) Mixing: First, add 50-60% of honey into a low-temperature emulsifier, then add the pre-treated bee pollen and royal jelly in sequence, and finally add the remaining honey, and stir at a low speed for at least 15 minutes to form a primary dispersion system; (3) Low-temperature emulsification: The temperature is raised to 50°C by a step-by-step heating method, and high-speed shear emulsification and low-speed stirring are performed alternately under low vacuum. The total duration of high-speed shear emulsification and low-speed stirring is at least 30 minutes. (4) Vacuum defoaming: vacuum defoaming is performed using a three-level vacuum gradient defoaming treatment, with each level of vacuum gradient maintained for at least 5 minutes; (5) Maturation: First, let it stand at room temperature for at least 24 hours, and then sterilize it with ultraviolet light to obtain the bee product compound preparation.

6. The low-temperature emulsification preparation method according to claim 5, characterized in that: The three-stage gradient wall breaking technology described in step (1) refers to first ultrafine wall breaking to a particle size of ≤40 μm, then wall breaking to a particle size of ≤20 μm, and finally wall breaking to a particle size of ≤5 μm.

7. The low-temperature emulsification preparation method according to claim 5, characterized in that: The stirring speed of the low-speed stirring in step (2) and step (3) is 25 to 35 rpm.

8. The low-temperature emulsification preparation method according to claim 7, characterized in that: The step-wise heating method in step (3) is to first heat the temperature from room temperature to 35°C, maintain it for at least 10 minutes, then heat it to 45°C, maintain it for at least 10 minutes, and then continue to heat it to 50°C; the low vacuum degree refers to a vacuum degree of -0.08MPa; the high-speed shear stirring speed is 8000rpm.

9. The low-temperature emulsification preparation method according to claim 5, characterized in that: The three levels of vacuum gradients described in step (4) are respectively -0.05MPa, -0.08MPa and -0.1MPa vacuum degrees.

10. The low-temperature emulsification preparation method according to claim 5, characterized in that: The ultraviolet light wavelength of the ultraviolet sterilization in step (5) is 254nm and the intensity is 30μW / cm 2 .