Diacylglycerol oil with blood sugar lowering effect and its preparation method

Abstract

The present invention provides a diglyceride oil with hypoglycemic effect and a preparation method thereof, which relates to the technical field of functional oil processing. The preparation method comprises the following steps: mixing seabuckthorn seed oil, Acer truncatum Bunge seed oil, rapeseed oil, corn oil and high-oleic acid peanut oil, adding glycerol and water to obtain material A; adding Pseudomonas cepacia lipase and Candida antarctica lipase B to material A for a primary reaction to obtain material B; adding Candida lipolytica lipase and Candida antarctica lipase B to material B for a second reaction to obtain material C; adding Thermomyces lanuginosus lipase to material C for a third reaction to obtain material D; and treating the reacted material D to obtain the diglyceride oil. The diglyceride oil prepared by the present invention has a good effect in reducing blood sugar.

Description

Technical Field

[0001] The invention relates to the technical field of functional oil processing, and in particular to diglyceride oil with blood sugar lowering effect and a preparation method thereof. Background Art

[0002] Diacylcerol is a structural lipid in which a fatty acid in triglyceride is replaced by a hydroxyl group. It is a trace component of natural plant oils and fats and an endogenous intermediate product of fat metabolism in the body. Diacylcerol oil exists in plant oils and is mainly composed of diacylglycerol. It has the effects of reducing visceral fat, inhibiting weight gain, and lowering blood lipids.

[0003] Blood sugar refers to the content of glucose in the blood. It is an important indicator for evaluating the normality of sugar metabolism. High or low blood sugar levels will lead to a series of symptoms. Usually, the normal value of fasting blood sugar is below 6.1mmol / L, and the normal value of blood sugar two hours after a meal is below 7.8mmol / L. If it exceeds this value, it is hyperglycemia. Long-term high blood sugar can cause lesions in various tissues and organs throughout the body, leading to the occurrence of adverse reactions such as acute and chronic complications such as dehydration, electrolyte imbalance, nutritional deficiencies and decreased resistance. Therefore, it is very important to control blood sugar. Existing technologies such as "Research Progress on the Effect of Diaglycerol Oil on Metabolic Syndrome" (Zhong Biying, Ding Yining, Wu Bingxin, etc., China Oils and Fats, 2022, 47(10):66-71) disclose that diglycerol oil can inhibit the increase of blood sugar after a meal, and summarize the effects of diglycerol oil on metabolic syndrome, including lowering blood sugar. In addition, "Research Progress on Physiological Functions and Preparation Technology of Diacylcerol" (Wang Yuehua, Cheng Fangyuan, Yang Xinyue, et al., Grain and Food Industry, 2024, 31(2):33-36) records the physiological functions of diacylglycerol, including but not limited to inhibiting the increase of blood lipids, inhibiting fat accumulation, and inhibiting the increase of blood sugar, etc., and also records the preparation method of diacylglycerol.

[0004] The current preparation methods of diglycerides mainly include enzymatic method and chemical method. The former has fewer by-products and the obtained diglycerides have high purity, while the latter has low preparation cost, but higher additional cost and will cause the degradation of heat-resistant polyunsaturated fatty acids. At present, the enzymatic method is mostly used for the preparation of diglycerides. The enzymatic method is specifically subdivided into esterification method, glycerolysis method, transesterification method and hydrolysis method, etc. The prior art such as Chinese Patent CN112322670A discloses a synthesis method of diglycerides, which includes the following steps: (1) mixing oil, lipase and solvent, and carrying out hydrolysis reaction to obtain a hydrolysis product; (2) under the action of diglyceride lipase, carrying out esterification reaction on the hydrolysis product obtained in step (1) with glycerol; the lipase in step (1) is lipase MAS1 or MAS1-H108A. The synthesis method of this invention can prepare diglycerides with high purity, and has simple operation and low cost. However, the lipase in this invention is mainly a single type of lipase derived from marine actinomycetes, which is added once, and the effect on diglyceride oil still has limitations. At the same time, the research on diglyceride oil in terms of blood sugar lowering has not been further carried out.

[0005] In view of the problems existing in the prior art, it is very necessary to find a diglyceride oil with blood sugar lowering effect and its preparation method. Summary of the Invention

[0006] In view of the problems existing in the prior art, the present invention provides a diglyceride oil with blood sugar lowering effect and its preparation method, and this diglyceride oil has good effect in blood sugar lowering.

[0007] To achieve the above object, the technical scheme adopted by the present invention is as follows:

[0008] The present invention provides a preparation method of a diglyceride oil with blood sugar lowering effect, including the following steps:

[0009] (1) Mixing seabuckthorn seed oil, Acer truncatum seed oil, rapeseed oil, corn oil and high oleic acid peanut oil as raw materials, and then adding glycerol and water to obtain material A;

[0010] (2) Adding Pseudomonas cepacia lipase and Candida antarctica lipase B to material A, and carrying out a primary reaction to obtain material B; the total weight of the Pseudomonas cepacia lipase and Candida antarctica lipase B is 0.1%-9% of the total weight of the seabuckthorn seed oil, Acer truncatum seed oil, rapeseed oil, corn oil and high oleic acid peanut oil; the weight ratio of the Pseudomonas cepacia lipase to Candida antarctica lipase B is 4-5:1;

[0011] (3) Add Candida lipolytica lipase and Candida antarctica lipase B to Material B for the second reaction to obtain Material C; the total weight of the Candida lipolytica lipase and Candida antarctica lipase B is 0.1%-6% of the total weight of the seabuckthorn seed oil, Acer truncatum seed oil, rapeseed oil, corn oil and high-oleic acid peanut oil; the weight ratio of the Candida lipolytica lipase to the Candida antarctica lipase B is 5-7:1;

[0012] (4) Add Thermomyces lanuginosus lipase to Material C for the third reaction to obtain Material D; the Thermomyces lanuginosus lipase is 0.1%-4% of the total weight of the seabuckthorn seed oil, Acer truncatum seed oil, rapeseed oil, corn oil and high-oleic acid peanut oil;

[0013] (5) Heat and centrifuge the reacted Material D, take the upper oil sample, and distill it under vacuum to obtain the diglyceride oil with blood sugar lowering effect.

[0014] Furthermore, in step (1), the weight of the glycerol is 20%-55% of the total weight of the seabuckthorn seed oil, Acer truncatum seed oil, rapeseed oil, corn oil and high-oleic acid peanut oil.

[0015] Even further, in step (1), the weight of the glycerol is 22%-55% of the total weight of the seabuckthorn seed oil, Acer truncatum seed oil, rapeseed oil, corn oil and high-oleic acid peanut oil, and the most preferred is 22%.

[0016] Furthermore, by weight, the raw materials include: 0.1-20 parts of seabuckthorn seed oil, 0.1-20 parts of Acer truncatum seed oil, 50-150 parts of rapeseed oil, 10-80 parts of corn oil and 5-20 parts of high-oleic acid peanut oil.

[0017] Even further, by weight, the raw materials include: 0.1-10 parts of seabuckthorn seed oil, 0.1-10 parts of Acer truncatum seed oil, 50-100 parts of rapeseed oil, 10-50 parts of corn oil and 5-10 parts of high-oleic acid peanut oil.

[0018] Furthermore, in step (2), the temperature of the first reaction is 40-50 °C and the time is 1-2 h; in step (3), the temperature of the second reaction is 40-50 °C and the time is 1-2 h; in step (4), the temperature of the third reaction is 35-50 °C and the time is 1-2 h.

[0019] Even further, in step (2), the temperature of the first reaction is 45 °C and the time is 1.5 h; in step (3), the temperature of the second reaction is 40 °C and the time is 1.5 h; in step (4), the temperature of the third reaction is 40 °C and the time is 1 h.

[0020] Further, the total weight of the Pseudomonas cepacia lipase and Candida antarctica lipase B in step (2) is 7% of the total weight of the sea buckthorn seed oil, Acer truncatum seed oil, rapeseed oil, corn oil, and high oleic acid peanut oil. The total weight of the Candida lipolytica lipase and Candida antarctica lipase B in step (3) is 5% of the total weight of the sea buckthorn seed oil, Acer truncatum seed oil, rapeseed oil, corn oil, and high oleic acid peanut oil. The Thermomyces lanuginosus lipase in step (4) is 3% of the total weight of the sea buckthorn seed oil, Acer truncatum seed oil, rapeseed oil, corn oil, and high oleic acid peanut oil.

[0021] Further, the weight ratio of the Pseudomonas cepacia lipase and Candida antarctica lipase B in step (2) is 4:1, and the weight ratio of the Candida lipolytica lipase and Candida antarctica lipase B in step (3) is 6:1.

[0022] Further, the temperature of the heating and centrifugation in step (5) is 80 - 110°C, and the time is 1.5 - 6 h.

[0023] Furthermore, the temperature of the heating and centrifugation in step (5) is 85°C, and the time is 5 h.

[0024] Further, the present invention also provides the diglyceride oil prepared by the above preparation method.

[0025] Further, the present invention provides that the diglyceride oil prepared by the above preparation method is used in the preparation of products for lowering blood sugar.

[0026] The technical effects achieved by the present invention are as follows:

[0027] (1) The present invention uses the glycerolysis process in the enzymatic hydrolysis method to prepare diglyceride oil. Specifically, by adopting the method of adding lipase in batches, and selecting the way of adding Pseudomonas cepacia lipase and Candida antarctica lipase B, Candida lipolytica lipase and Candida antarctica lipase B, and Thermomyces lanuginosus lipase in batches, the diglyceride oil with excellent performance can be obtained more precisely, avoiding the generation of triglycerides, so that the obtained diglyceride oil has a high yield and a high content.

[0028] (2) The present invention selects specific raw materials and enzymes for preparation, with a shorter preparation time and lower cost, and is suitable for continuous production at the industrial application level.

[0029] (3) The diglyceride oil prepared by the present invention has a good blood sugar lowering effect. Detailed Embodiments

[0030] The following describes the embodiments of the present invention through specific examples. Those skilled in the art can easily understand the other advantages and effects of the present invention from the content disclosed in this specification. The present invention can also be implemented or applied through other different specific embodiments. Various details in this specification can also be modified or changed based on different viewpoints and applications without departing from the spirit of the present invention.

[0031] Before further describing the specific embodiments of the present invention, it should be understood that the protection scope of the present invention is not limited to the specific embodiments described below; it should also be understood that the terms used in the embodiments of the present invention are for describing specific embodiments, rather than limiting the protection scope of the present invention.

[0032] When the embodiments give a numerical range, it should be understood that unless otherwise specified in the present invention, both endpoints of each numerical range and any value between the two endpoints can be selected. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the technical field to which the present invention belongs.

[0033] It is worth noting that the Pseudomonas cepacia lipase used in the present invention is purchased from Hangzhou Chuangke Biotechnology Co., Ltd., with an enzyme activity of 30000 U / g. The Candida antarctica lipase B is purchased from Hangzhou Chuangke Biotechnology Co., Ltd., with an enzyme activity of 10000 U / g. The Candida lipolytica lipase is purchased from Wuxi Enzyme Preparation Factory, with an enzyme activity of 4500 U / g. The Thermomyces lanuginosus lipase is the immobilized lipase Thermomyces lanuginosus Lipozyme TLIM of Novozymes, with a transesterification activity of 250 IUN / g. The sea buckthorn seed oil is purchased from Heze Zhonghe Jianyuan Biotechnology Co., Ltd., with the product number 08T20B48801. The Acer truncatum seed oil is purchased from Heze Zhonghe Jianyuan Biotechnology Co., Ltd., with the product number 22T20B48801. The rapeseed oil is purchased from Chengdu Xinxing Grain and Oil Co., Ltd. The corn oil is purchased from Shandong Yuhuang Grain and Oil Food Co., Ltd., with the product number PY240916107. The high oleic acid peanut oil is purchased from Shandong Jinsheng Grain and Oil Food Co., Ltd., with the product number GY2405. The remaining raw materials are all ordinary commercially available products, and their sources are not specifically limited.

[0034] Example 1

[0035] (1) By weight, 0.5 parts of sea buckthorn seed oil, 0.5 parts of Acer truncatum seed oil, 80 parts of rapeseed oil, 10 parts of corn oil, and 9 parts of high oleic acid peanut oil are fully mixed, and then 22 parts by weight of glycerol and 10 parts by weight of water are added and fully mixed evenly to obtain Material A;

[0036] (2) First reaction: Add a mixture of 7 parts by weight of Pseudomonas cepacia lipase and Candida antarctica lipase B to Material A for the first reaction. The reaction temperature is 45°C and the time is 1.5 h to obtain Material B. The weight ratio of Pseudomonas cepacia lipase to Candida antarctica lipase B is 4:1.

[0037] (3) Second reaction: Add a mixture of 5 parts by weight of Candida lipolytica lipase and Candida antarctica lipase B to Material B for the second reaction. The reaction temperature is 40°C and the time is 1.5 h to obtain Material C. The weight ratio of Candida lipolytica lipase to Candida antarctica lipase B is 6:1.

[0038] (4) Third reaction: Add 3 parts by weight of Thermomyces lanuginosus lipase to Material C for the third reaction. The reaction temperature is 40°C and the time is 1 h to obtain Material D.

[0039] (5) Heat and centrifuge the reacted Material D at 85°C for 5 h. Take the upper layer oil sample for molecular distillation. The evaporation surface temperature is 120°C, the feeding rate is 10 kg / min, the scraper speed is 200 r / min, and the vacuum degree is 0.1 Pa to obtain diglyceride oil.

[0040] Example 2

[0041] (1) According to parts by weight, fully mix 0.5 part of seabuckthorn seed oil, 0.5 part of Acer truncatum seed oil, 80 parts of rapeseed oil, 10 parts of corn oil, and 9 parts of high-oleic acid peanut oil, and then add 20 parts by weight of glycerol and 10 parts by weight of water and mix well to obtain Material A.

[0042] (2) First reaction: Add a mixture of 5 parts by weight of Pseudomonas cepacia lipase and Candida antarctica lipase B to Material A for the first reaction. The reaction temperature is 50°C and the time is 1 h to obtain Material B. The weight ratio of Pseudomonas cepacia lipase to Candida antarctica lipase B is 5:1.

[0043] (3) Second reaction: Add a mixture of 6 parts by weight of Candida lipolytica lipase and Candida antarctica lipase B to Material B for the second reaction. The reaction temperature is 50°C and the time is 1 h to obtain Material C. The weight ratio of Candida lipolytica lipase to Candida antarctica lipase B is 5:1.

[0044] (4) Third reaction: Add 2 parts by weight of Thermomyces lanuginosus lipase to Material C for the third reaction. The reaction temperature is 35°C and the time is 2 h to obtain Material D.

[0045] (5) The reacted material D was heated and centrifuged at 85°C for 5 h. The upper layer of oil was sampled and molecularly distilled at an evaporation surface temperature of 120°C, a feed rate of 10 kg / min, a scraper speed of 200 r / min, and a vacuum degree of 0.1 Pa to obtain diglyceride oil.

[0046] Example 3

[0047] (1) After fully mixing 0.5 parts of seabuckthorn seed oil, 0.5 parts of maple seed oil, 80 parts of rapeseed oil, 10 parts of corn oil, and 9 parts of high oleic peanut oil according to weight parts, 25 parts of glycerin and 10 parts of water were added, and the mixture was fully mixed to obtain material A;

[0048] (2) Primary reaction: adding 9 parts by weight of a mixture of Pseudomonas cepacia lipase and Candida antarctica lipase B to material A, and conducting a primary reaction at a temperature of 40° C. for 2 h to obtain material B; the weight ratio of the Pseudomonas cepacia lipase to the Candida antarctica lipase B is 4:1;

[0049] (3) Second reaction: adding 3 parts by weight of a mixture of Candida lipolytica lipase and Candida antarctica lipase B to material B, and conducting a second reaction at a temperature of 40° C. for 2 h to obtain material C; the weight ratio of the Candida lipolytica lipase to the Candida antarctica lipase B is 7:1;

[0050] (4) Third reaction: 4 parts by weight of Thermomyces lanuginosus lipase was added to material C, and the third reaction was carried out at a temperature of 50° C. for 1 h to obtain material D;

[0051] (5) The reacted material D was heated and centrifuged at 85°C for 5 h. The upper layer of oil was sampled and molecularly distilled at an evaporation surface temperature of 120°C, a feed rate of 10 kg / min, a scraper speed of 200 r / min, and a vacuum degree of 0.1 Pa to obtain diglyceride oil.

[0052] Comparative Example 1

[0053] The difference from Example 1 is that in the first reaction, 10 parts by weight of a mixture of Pseudomonas cepacia lipase and Candida antarctica lipase B are added; in the second reaction, 2 parts by weight of a mixture of Candida lipolytica lipase and Candida antarctica lipase B are added; in the third reaction, 5 parts by weight of Thermomyces lanuginosus lipase are added, and the rest are the same as in Example 1.

[0054] Comparative Example 2

[0055] Different from Example 1: In the first reaction, a mixture of 3 parts by weight of Pseudomonas cepacia lipase and Candida antarctica lipase B was added; in the second reaction, a mixture of 8 parts by weight of Candida lipolytica lipase and Candida antarctica lipase B was added; in the third reaction, 1 part by weight of Thermomyces lanuginosus lipase was added, and the rest was the same as in Example 1.

[0056] Comparative Example 3

[0057] (1) According to parts by weight, 0.5 part of sea buckthorn seed oil, 0.5 part of Acer truncatum seed oil, 80 parts of rapeseed oil, 10 parts of corn oil, and 9 parts of high-oleic acid peanut oil were fully mixed, and then 22 parts by weight of glycerol and 10 parts by weight of water were added and fully mixed evenly to obtain Material A;

[0058] (2) First reaction: 7 parts by weight of Candida antarctica lipase B was added to Material A for the first reaction. The reaction temperature was 45 °C and the time was 1.5 h to obtain Material B;

[0059] (3) Second reaction: 5 parts by weight of Candida antarctica lipase B was added to Material B for the second reaction. The reaction temperature was 40 °C and the time was 1.5 h to obtain Material C;

[0060] (4) Third reaction: 3 parts by weight of Thermomyces lanuginosus lipase was added to Material C for the third reaction. The reaction temperature was 40 °C and the time was 1 h to obtain Material D;

[0061] (5) The reacted Material D was heated and centrifuged at 85 °C for

[0062] 5 h, and the upper oil sample was taken for molecular distillation. The evaporation surface temperature was 120 °C, the feeding rate was 10 kg / min, the scraper rotation speed was 200 r / min, and the vacuum degree was 0.1 Pa to obtain diglyceride oil.

[0063] Comparative Example 4

[0064] (1) According to parts by weight, 0.5 part of sea buckthorn seed oil, 0.5 part of Acer truncatum seed oil, 80 parts of rapeseed oil, 10 parts of corn oil, and 9 parts of high-oleic acid peanut oil were fully mixed, and then 22 parts by weight of glycerol and 10 parts by weight of water were added and fully mixed evenly to obtain Material A;

[0065] (2) First reaction: A mixture of 7 parts by weight of Pseudomonas cepacia lipase and Candida antarctica lipase B was added to Material A for the first reaction. The reaction temperature was 45 °C and the time was 1.5 h to obtain Material B; the weight ratio of Pseudomonas cepacia lipase to Candida antarctica lipase B was 4:1;

[0066] (3) Second reaction: Add 5 parts by weight of a mixture of Pseudomonas cepacia lipase and Candida antarctica lipase B to Material B, and conduct the second reaction at a temperature of 40 °C for 1.5 h to obtain Material C; the weight ratio of Pseudomonas cepacia lipase to Candida antarctica lipase B is 4:1;

[0067] (4) Third reaction: Add 3 parts by weight of a mixture of Pseudomonas cepacia lipase and Candida antarctica lipase B to Material C, and conduct the third reaction at a temperature of 40 °C for 1 h to obtain Material D; the weight ratio of Pseudomonas cepacia lipase to Candida antarctica lipase B is 4:1;

[0068] (5) Heat and centrifuge the reacted Material D at 85 °C for 5 h, take the upper oil sample, and conduct molecular distillation at an evaporation surface temperature of 120 °C, a feed rate of 10 kg / min, a scraper speed of 200 r / min, and a vacuum degree of 0.1 Pa to obtain diglyceride oil.

[0069] Comparative Example 5

[0070] (1) According to parts by weight, fully mix 0.5 part of sea buckthorn seed oil, 0.5 part of Acer truncatum seed oil, 80 parts of rapeseed oil, 10 parts of corn oil, and 9 parts of high-oleic acid peanut oil, then add 22 parts by weight of glycerol and 10 parts by weight of water, and mix well to obtain Material A;

[0071] (2) Primary reaction: Add 7 parts by weight of Thermomyces lanuginosus lipase to Material A for the primary reaction at a temperature of 45 °C for 1.5 h to obtain Material B;

[0072] (3) Second reaction: Add 5 parts by weight of a mixture of Pseudomonas cepacia lipase and Candida antarctica lipase B to Material B, and conduct the second reaction at a temperature of 40 °C for 1.5 h to obtain Material C; the weight ratio of Pseudomonas cepacia lipase to Candida antarctica lipase B is 4:1;

[0073] (4) Third reaction: Add 3 parts by weight of a mixture of Candida lipolytica lipase and Candida antarctica lipase B to Material C, and conduct the third reaction at a temperature of 40 °C for 1 h to obtain Material D; the weight ratio of Candida lipolytica lipase to Candida antarctica lipase B is 6:1;

[0074] (5) Heat and centrifuge the reacted Material D at 85 °C for 5 h, take the upper oil sample, and conduct molecular distillation at an evaporation surface temperature of 120 °C, a feed rate of 10 kg / min, a scraper speed of 200 r / min, and a vacuum degree of 0.1 Pa to obtain diglyceride oil.

[0075] Comparative Example 6

[0076] The material C obtained in Example 1 was heated and centrifuged at 85°C for 5 h. The upper-layer oil sample was taken and subjected to molecular distillation. The evaporation surface temperature was 120°C, the feeding rate was 10 kg / min, the scraper rotation speed was 200 r / min, and the vacuum degree was 0.1 Pa to obtain diglyceride oil.

[0077] Comparative Example 7

[0078] The difference from Example 1 was that the raw material was 100 parts of whole corn oil, and the rest were the same as in Example 1.

[0079] Comparative Example 8

[0080] The difference from Example 1 was that the raw material was 100 parts of whole rapeseed oil, and the rest were the same as in Example 1.

[0081] Result Detection

[0082] 1. Detection of diglyceride content

[0083] The diglyceride content was determined according to GB / T 26636. The upper-layer oil sample before molecular distillation and the diglyceride oil obtained after molecular distillation were detected respectively.

[0084] The detection results are shown in Table 1:

[0085] Table 1

[0086]

[0087] Generally, the molecular free paths of diglyceride and triglyceride are very similar. It is difficult to completely separate them by molecular distillation, resulting in a low diglyceride content. In the present invention, enzymatic hydrolysis is carried out in three steps, and a suitable lipase is selected in each step to obtain diglyceride with high selectivity, avoiding the problem of insufficient purity caused by the difficulty in separating diglyceride and triglyceride.

[0088] The results of Comparative Example 1 and Comparative Example 2 show that a suitable enzyme addition amount has an obvious influence on the composition of the enzymatic hydrolysis product. In the experiments of Comparative Examples 3 - 5, different enzymes were tried in each step. As a result, the diglyceride content was much lower than that of Examples 1 - 3. After the material obtained in the second step of Comparative Example 6 was distilled and purified, the content was slightly lower than that of Example 1, but the purity before distillation was significantly lower than that of Examples 1 - 3, indicating that there would be a large yield loss in the preparation process of Comparative Example 6. Comparative Examples 7 and 8 used a single kind of oil for preparation, and the content was also significantly different from that of Example 1.

[0089] 2. Detection of blood sugar lowering effect

[0090] Experimental animals: BKS-DB male clean-grade mice (genotype mut / mut), 18 - 22 g. The mice were randomly divided into a model group, a high-dose group of Example 1, a medium-dose group of Example 1, and a low-dose group of Example 1 according to their fasting blood glucose levels. Additionally, BKS clean-grade mice (genotype wt / wt) were used as a blank group, with 10 mice in each group.

[0091] Experimental method: After one week of adaptive feeding, the high-dose group of Example 1, the medium-dose group of Example 1, and the low-dose group of Example 1 were given intragastric administration once a day, with the administration doses being 0.1 mL / 10 g, 0.06 mL / 10 g, and 0.03 mL / 10 g respectively. The blank group and the model group were given an equal amount of normal saline by gavage every day. The blood glucose levels of the mice were measured on the 0th, 45th, and 90th days after administration.

[0092] The results are shown in Table 2:

[0093] Table 2

[0094]

[0095] Note: When comparing the model group with the blank group, **P < 0.01; when comparing the experimental groups (the high-dose group of Example 1, the medium-dose group of Example 1, and the low-dose group of Example 1) with the model group, ## P < 0.01; # P < 0.05.

[0096] The results show that the diglyceride oil prepared in Example 1 has a significant blood glucose-lowering effect. In addition, the blood glucose-lowering effects of the high-dose group of Example 1 on the 45th and 90th days are better than those of the medium-dose group of Example 1 and the low-dose group of Example 1.

[0097] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, rather than to limit the protection scope of the present invention. Any simple modification or equivalent replacement made by those of ordinary skill in the art to the technical solution of the present invention shall not depart from the essence and scope of the technical solution of the present invention.

Claims

1. A preparation method of diglyceride oil with hypoglycemic effect, characterized in that, It includes the following steps: (1) After mixing seabuckthorn seed oil, Acer truncatum Bunge seed oil, rapeseed oil, corn oil and high-oleic acid peanut oil as raw materials, glycerol and water are added to obtain material A; by weight parts, the raw materials include: 0.1-10 parts of seabuckthorn seed oil, 0.1-10 parts of Acer truncatum Bunge seed oil, 50-100 parts of rapeseed oil, 10-50 parts of corn oil and 5-10 parts of high-oleic acid peanut oil; the weight of the glycerol is 20%-55% of the total weight of the seabuckthorn seed oil, Acer truncatum Bunge seed oil, rapeseed oil, corn oil and high-oleic acid peanut oil; (2) Pseudomonas cepacia lipase and Candida antarctica lipase B are added to material A for the first reaction to obtain material B; the total weight of the Pseudomonas cepacia lipase and Candida antarctica lipase B is 5%-9% of the total weight of the seabuckthorn seed oil, Acer truncatum Bunge seed oil, rapeseed oil, corn oil and high-oleic acid peanut oil; the weight ratio of the Pseudomonas cepacia lipase to Candida antarctica lipase B is 4-5:1; (3) Candida lipolytica lipase and Candida antarctica lipase B are added to material B for the second reaction to obtain material C; the total weight of the Candida lipolytica lipase and Candida antarctica lipase B is 3%-6% of the total weight of the seabuckthorn seed oil, Acer truncatum Bunge seed oil, rapeseed oil, corn oil and high-oleic acid peanut oil; the weight ratio of the Candida lipolytica lipase to Candida antarctica lipase B is 5-7:1; (4) Thermomyces lanuginosus lipase is added to material C for the third reaction to obtain material D; the Thermomyces lanuginosus lipase is 2%-4% of the total weight of the seabuckthorn seed oil, Acer truncatum Bunge seed oil, rapeseed oil, corn oil and high-oleic acid peanut oil; (5) The reacted material D is heated and centrifuged, and the upper oil sample is taken and distilled under vacuum to obtain the diacylglycerol oil with blood sugar lowering efficacy; In step (2), the temperature of the first reaction is 40-50°C and the time is 1-2 h; in step (3), the temperature of the second reaction is 40-50°C and the time is 1-2 h; in step (4), the temperature of the third reaction is 35-50°C and the time is 1-2 h.

2. The preparation method according to claim 1, characterized in that, In step (2), the temperature of the first reaction is 45°C and the time is 1.5 h; in step (3), the temperature of the second reaction is 40°C and the time is 1.5 h; in step (4), the temperature of the third reaction is 40°C and the time is 1 h.

3. The preparation method according to claim 1, characterized in that, In step (2), the total weight of the Pseudomonas cepacia lipase and Candida antarctica lipase B is 7% of the total weight of the seabuckthorn seed oil, Acer truncatum Bunge seed oil, rapeseed oil, corn oil and high-oleic acid peanut oil; in step (3), the total weight of the Candida lipolytica lipase and Candida antarctica lipase B is 5% of the total weight of the seabuckthorn seed oil, Acer truncatum Bunge seed oil, rapeseed oil, corn oil and high-oleic acid peanut oil; in step (4), the Thermomyces lanuginosus lipase is 3% of the total weight of the seabuckthorn seed oil, Acer truncatum Bunge seed oil, rapeseed oil, corn oil and high-oleic acid peanut oil.

4. The preparation method according to claim 1, characterized in that, The weight ratio of the Pseudomonas cepacia lipase and Candida antarctica lipase B described in step (2) is 4:1, and the weight ratio of the Candida lipolytica lipase and Candida antarctica lipase B described in step (3) is 6:

1.

5. The preparation method according to claim 1, characterized in that, The weight of the glycerol described in step (1) is 22%-55% of the total weight of the sea buckthorn seed oil, Acer truncatum seed oil, rapeseed oil, corn oil and high-oleic acid peanut oil.

6. The diglyceride oil prepared by the preparation method according to any one of claims 1-5.

Images