Traditional Chinese medicine composition for treating thin endometrium as well as preparation method and application of traditional Chinese medicine composition

Abstract

The invention discloses a traditional Chinese medicine composition for treating thin endometrium as well as a preparation method and application of the traditional Chinese medicine composition. The traditional Chinese medicine composition is mainly prepared from tortoise-plastron glue, fructus lycii, semen cuscutae, rhizoma sparganii, semen persicae, fructus polygoni orientalis, sargentgloryvine stem, radix dipsaci, cortex eucommiae and cassia twig. The invention further discloses a preparation method of the traditional Chinese medicine composition, a pharmaceutical preparation and application. Pharmacodynamic experiments and clinical application prove that the traditional Chinese medicine composition can be used for treating thin endometrium, the thickness of the endometrium and the wet weight of the endometrium can be increased, and the number of endometrial glands can be increased, so that the receptivity of the endometrium is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicine. More specifically, it relates to a traditional Chinese medicine composition for treating thin endometrium, its preparation method and application. Background Art

[0002] Patients after fertility-sparing treatment for endometrial cancer mostly show thin endometrium during ovulation. This may be due to the use of high-dose progesterone or repeated hysteroscopic surgeries, which cause endometrial damage and decreased endometrial receptivity, resulting in failed embryo implantation. Endometrial receptivity describes the complex process of the endometrium preparing for embryo implantation and is a key factor for successful pregnancy. Successful implantation and early pregnancy maintenance depend on endometrial receptivity. The lack or absence of endometrial receptivity leads to early pregnancy loss and infertility. Research shows that poor endometrial receptivity accounts for 2 / 3 of the reasons for failure in assisted reproductive technology. The establishment of endometrial receptivity is a key factor in embryo implantation, and endometrial thickness is considered a key indicator for evaluating endometrial receptivity. The treatment strategies for endometrial cancer combined with thin endometrium mainly include: (1) Estrogen stimulation of the endometrium: In most cases, estrogen treatment is the first choice for patients with thin endometrium to promote endometrial regeneration. Estradiol at supra-physiological doses can prolong the growth time of the endometrium and is beneficial for increasing the endometrial thickness. However, long-term use of high-dose estrogen may increase the risk of endometrial cancer recurrence and thrombosis. (2) Intrauterine perfusion of platelet-rich plasma (PRP), but there is still controversy about the treatment effect on endometrial damage at present, and there is no unified clinical standard for the specific damage range, degree and surgical timing. (3) Pelvic floor bioelectrical stimulation therapy. Considering that patients with refractory thin endometrium have poor endometrial blood perfusion, the high blood flow impedance of the uterine artery may lead to impaired growth of endometrial glandular epithelium and a decrease in the VEGF level of endometrial blood vessels, resulting in poor blood vessel development, which may further reduce endometrial blood flow, form a vicious cycle, and lead to thinning of the endometrium.

[0003] Therefore, it is necessary to find a safer and more effective way to improve the thin endometrium after fertility-sparing treatment for endometrial cancer. Summary of the Invention

[0004] Aiming at the above technical problems, an object of the present invention is to provide a new traditional Chinese medicine composition, which can be used to treat thin endometrium, increase the endometrial thickness and wet weight, and increase the number of endometrial glands, thereby improving endometrial receptivity.

[0005] Another object of the present invention is to provide a preparation method of the above traditional Chinese medicine composition.

[0006] Still another object of the present invention is to provide a traditional Chinese medicine preparation containing the above traditional Chinese medicine composition.

[0007] Another object of the present invention is to provide the use of the above-mentioned Chinese medicine composition and its pharmaceutical preparation in the preparation of a drug for treating thin endometrium.

[0008] In order to achieve the above object, the present invention adopts the following technical solutions:

[0009] In a first aspect, the present invention provides a traditional Chinese medicine composition for treating thin endometrium, wherein the traditional Chinese medicine composition is mainly made of the following raw materials: tortoise shell glue, wolfberry fruit, dodder seed, triangularis, peach kernel, polyanthus orientalis fruit, Sargentodoxae, Dipsacus asper, Eucommia ulmoides, and cassia twig.

[0010] Preferably, the traditional Chinese medicine composition is mainly made of the following raw materials in parts by weight: 5-10 parts of tortoise shell glue, 9-15 parts of wolfberry, 15-30 parts of dodder seed, 9-15 parts of triangularis, 9-12 parts of peach kernel, 9-15 parts of polyanthus orientalis, 9-15 parts of Sargentodoxa chinensis, 9-15 parts of Dipsacus asper, 9-15 parts of Eucommia ulmoides, and 6-9 parts of cassia twig.

[0011] The uterus is a "female uterus" in traditional Chinese medicine. Its main functions are to host menstruation and nurture the fetus. According to the symptoms caused by thin endometrium, traditional Chinese medicine often refers to "oligomenorrhea" and "infertility" for diagnosis and treatment. Female uterus is a female reproductive organ, and its development and maturity depend on the "heavenly essence" generated by the essence and qi in the kidney. "Suwen: Ancient Innocence" states that "at the age of 27, the heavenly essence arrives, the Ren meridian is open, the Taichong meridian is strong, the menstruation comes on time, so there are children... At the age of 77, the Ren meridian is weak, the Taichong meridian is weak, the heavenly essence is exhausted, the earth channel is blocked, so the body is damaged and there are no children." If the kidney is full of essence and qi, and the Chong and Ren meridians are vigorous, then the menstruation is normal; if the kidney is deficient and the Chong and Ren meridians are weak, then there will be irregular menstruation and infertility. Women are based on blood. If they are affected by evil spirits, the evil spirits and blood will form blood stasis. Or if they are always depressed, there will be qi stagnation and blood stasis, which will block Chong and Ren meridians and cause blood circulation problems, leading to scanty menstruation. Or if there is blood stasis in the cells, qi and blood will not flow smoothly and cannot nourish the endometrium, causing the endometrium to be too thin and infertility to occur.

[0012] Thin endometrium belongs to the syndrome of kidney deficiency and blood stasis. In the prescription of the present invention, tortoise plastron glue and wolfberry fruit are used as the monarch drugs together. Tortoise plastron glue nourishes the kidney and replenishes essence, greatly tonifying the true yin; wolfberry fruit nourishes the liver and kidney, benefits essence and nourishes blood, assisting tortoise plastron glue to enhance the power of tonifying the kidney and replenishing essence. Sanleng (Rhizoma Sparganii), Taoren (Semen Persicae), Fructus Polygoni Orientalis, and Caulis Sargentodoxae are used as the minister drugs. Sanleng, Taoren, and Fructus Polygoni Orientalis promote blood circulation to remove stasis, break qi and remove stasis, disperse stasis in the uterine cavity, and improve local microcirculation in the ovary; Caulis Sargentodoxae promotes blood circulation to dredge collaterals, clears heat and detoxifies, prevents heat transformation due to long-term stasis, and promotes follicle development and discharge. Cuscuta chinensis, Dipsacus asperoides, and Eucommia ulmoides are used as the assistant drugs. Cuscuta chinensis tonifies both yin and yang, benefits essence and secures the fetus; Dipsacus asperoides and Eucommia ulmoides tonify the liver and kidney, strengthen the tendons and bones, regulate the thoroughfare and conception vessels, and stabilize the kidney qi, jointly assisting the monarch drug to tonify the kidney. Cinnamon twig warms and dredges the meridians, promotes yang qi transformation, harmonizes yin and yang in the kidney, takes both warming and dredging and astringency into account, assisting the effects of the monarch and minister drugs to play, and at the same time serving as the guiding drug, and its pungent-warm property leads all the drugs into the blood vessels and uterine cavity. The combination of all the drugs benefits the kidney and promotes blood circulation, making the kidney qi sufficient, the blood circulation unobstructed, and the endometrium nourished.

[0013] Exemplarily, the traditional Chinese medicine composition may be mainly prepared from the following raw materials in parts by weight: 5 parts of tortoise plastron glue, 12 parts of wolfberry fruit, 30 parts of Cuscuta chinensis, 9 parts of Sanleng (Rhizoma Sparganii), 9 parts of Taoren (Semen Persicae), 12 parts of Fructus Polygoni Orientalis, 15 parts of Caulis Sargentodoxae, 9 parts of Dipsacus asperoides, 15 parts of Eucommia ulmoides, and 6 parts of cinnamon twig.

[0014] Exemplarily, the traditional Chinese medicine composition may be mainly prepared from the following raw materials in parts by weight: 10 parts of tortoise plastron glue, 15 parts of wolfberry fruit, 30 parts of Cuscuta chinensis, 15 parts of Sanleng (Rhizoma Sparganii), 12 parts of Taoren (Semen Persicae), 15 parts of Fructus Polygoni Orientalis, 15 parts of Caulis Sargentodoxae, 15 parts of Dipsacus asperoides, 15 parts of Eucommia ulmoides, and 9 parts of cinnamon twig.

[0015] Exemplarily, the traditional Chinese medicine composition may be mainly prepared from the following raw materials in parts by weight: 5 parts of tortoise plastron glue, 9 parts of wolfberry fruit, 15 parts of Cuscuta chinensis, 9 parts of Sanleng (Rhizoma Sparganii), 9 parts of Taoren (Semen Persicae), 9 parts of Fructus Polygoni Orientalis, 9 parts of Caulis Sargentodoxae, 9 parts of Dipsacus asperoides, 9 parts of Eucommia ulmoides, and 6 parts of cinnamon twig.

[0016] The raw materials of the above traditional Chinese medicine composition of the present invention form the basic formula, and according to the actual clinical symptoms, it can also be adjusted on the basis of the above traditional Chinese medicine composition to enhance the adaptability of the composition of the present invention and improve the treatment effect.

[0017] In a second aspect, the present invention provides a preparation method of the above traditional Chinese medicine composition for treating thin endometrium, and the preparation method includes the following steps:

[0018] Weigh wolfberry fruit, Cuscuta chinensis, Sanleng (Rhizoma Sparganii), Taoren (Semen Persicae), Fructus Polygoni Orientalis, Caulis Sargentodoxae, Dipsacus asperoides, Eucommia ulmoides, and cinnamon twig according to the formula amount, soak them in water for 30 - 40 minutes, boil them over high heat and then simmer them, filter to obtain the first filtrate, and keep the medicinal residues;

[0019] Boil the medicinal residues again in water and then simmer them, filter to obtain the second filtrate;

[0020] Combine the two filtrates;

[0021] Melt the tortoise plastron glue and set aside;

[0022] Pour the melted tortoise plastron glue into the combined filtrate to obtain the product.

[0023] Furthermore, in the above preparation method, the amount of water added for each decoction is 10 - 15 times the total weight of the raw medicinal materials. The amount of water added for the two decoctions can be the same or different; the time for each decoction is 30 - 90 min, and the time for each decoction can be the same or different.

[0024] Furthermore, for the convenience of storage and for use in making various pharmaceutical dosage forms, the above preparation method may further include the step of concentrating the filtrate into a clear extract.

[0025] Furthermore, the clear extract is a clear extract with a relative density of 1.0 - 1.3 measured at 50°C - 60°C.

[0026] In a third aspect, the present invention provides a traditional Chinese medicine preparation for treating thin endometrium, and the traditional Chinese medicine preparation uses the above traditional Chinese medicine composition or the traditional Chinese medicine composition prepared by the above preparation method as an active ingredient.

[0027] Furthermore, according to the actual dosage form requirements, the traditional Chinese medicine preparation may further include pharmaceutically acceptable excipients, such as binders, disintegrants, lubricants, fillers, suspending agents, etc. According to the conventional preparation process, various preparations are made, such as decoctions, granules, pills, capsules, tablets, powders, oral liquids, etc.

[0028] The present invention also provides the application of the above traditional Chinese medicine composition or traditional Chinese medicine preparation in the preparation of a drug for treating thin endometrium.

[0029] In addition, unless otherwise specified, each raw medicinal material of the traditional Chinese medicine composition of the present invention can be obtained through commercial purchase. Any range described in the present invention includes the end values and any numerical value between the end values, and any sub - range composed of any numerical value between the end values or the end values can achieve the purpose of treating thin endometrium.

[0030] The beneficial effects of the present invention are as follows:

[0031] The traditional Chinese medicine composition of the present invention can effectively treat thin endometrium, can increase the endometrial thickness, endometrial wet weight, increase the number of endometrial glands, up - regulate the expression level of the endometrial receptivity marker LIF, and improve endometrial receptivity. In particular, it can effectively improve the thin endometrium after fertility - sparing treatment for endometrial cancer. Description of the Drawings

[0032] Figure 1Show the effect of the traditional Chinese medicine composition of the present invention on the endometrial thickness of TE rats (unit: μm).

[0033] Figure 2 Show the effect of the traditional Chinese medicine composition of the present invention on the wet weight of the endometrium of TE rats (unit: g).

[0034] Figure 3 Show the effect of the traditional Chinese medicine composition of the present invention on the number of endometrial glands of TE rats (unit: pieces).

[0035] Figure 4 Show the effect of the traditional Chinese medicine composition of the present invention on the expression level of LIF in the endometrium of TE rats. Detailed implementation manners

[0036] To illustrate the present invention more clearly, the present invention will be further described below in conjunction with preferred embodiments. Those skilled in the art should understand that the content specifically described below is illustrative rather than restrictive, and should not be used to limit the protection scope of the present invention.

[0037] Example 1 A traditional Chinese medicine composition for treating thin endometrium

[0038] Raw material drug formula: 5 g of tortoise shell glue, 12 g of wolfberry fruit, 30 g of dodder seeds, 9 g of rhizoma sparganii, 9 g of peach kernels, 12 g of polygonum orientale, 15 g of sargentodoxa cuneata, 9 g of dipsacus asperoides, 15 g of eucommia ulmoides, 6 g of cassia twig.

[0039] Except for tortoise shell glue, soak the remaining raw material drugs in water, boil them, and then decoct them over a slow fire twice. For the first time, add 10 times the total weight of the raw material drugs of water, decoct for 40 minutes, filter to obtain the filtrate; for the second time, add the drug residues to 10 times the total weight of the raw material drugs of water, decoct for 30 minutes, filter to obtain the second filtrate; combine the two filtrates; melt the tortoise shell glue for later use; pour the melted tortoise shell glue into the combined filtrate to obtain it.

[0040] Example 2 A traditional Chinese medicine composition for treating thin endometrium

[0041] Raw material drug formula: 10 g of tortoise shell glue, 15 g of wolfberry fruit, 30 g of dodder seeds, 15 g of rhizoma sparganii, 12 g of peach kernels, 15 g of polygonum orientale, 15 g of sargentodoxa cuneata, 15 g of dipsacus asperoides, 15 g of eucommia ulmoides, 9 g of cassia twig.

[0042] Except for tortoise shell glue, soak the remaining raw material drugs in water, boil them, and then decoct them over a slow fire twice. For the first time, add 12 times the total weight of the raw material drugs of water, decoct for 90 minutes, filter to obtain the filtrate; for the second time, add the drug residues to 10 times the total weight of the raw material drugs of water, decoct for 60 minutes, filter and combine the two filtrates, melt the tortoise shell glue, pour the melted tortoise shell glue into the combined filtrate, and then concentrate it into a clear paste (a clear paste with a relative density of 1.24 - 1.30 measured at 50 °C), and dry it under reduced pressure to obtain it.

[0043] Example 3 A traditional Chinese medicine preparation (granules) for treating thin endometrium

[0044] Formula of raw materials: 5 g of tortoise plastron glue, 9 g of wolfberry fruit, 15 g of dodder seeds, 9 g of rhizoma sparganii, 9 g of peach kernels, 9 g of polygonum orientale fruit, 9 g of sargentodoxa cuneata, 9 g of dipsacus asperoides, 9 g of eucommia ulmoides, 6 g of cassia twig.

[0045] The preparation method is the same as that in Example 2, and further includes making the extract into granules according to the granule process.

[0046] Example 4 Efficacy experiment of the traditional Chinese medicine composition of the present invention in treating thin endometrium

[0047] Animal experiment

[0048] 1 Experimental animals

[0049] 70 SPF-grade healthy adult female virgin SD rats, with a body weight of 220 ± 20 g, from which 60 female rats with regular estrous cycles were selected, and 20 SPF-grade healthy adult male SD rats, with a body weight of 250 ± 20 g.

[0050] 2 Experimental drugs

[0051] (1) Traditional Chinese medicine: 5 g of tortoise plastron glue, 12 g of wolfberry fruit, 30 g of dodder seeds, 9 g of rhizoma sparganii, 9 g of peach kernels, 12 g of polygonum orientale fruit, 15 g of sargentodoxa cuneata, 9 g of dipsacus asperoides, 15 g of eucommia ulmoides, 6 g of cassia twig (total 122 g). The Chinese herbal pieces were purchased from the Chinese pharmacy of Guang'anmen Hospital, China Academy of Chinese Medical Sciences. Except for tortoise plastron glue, the remaining Chinese herbs were added with 10 times the volume of water and decocted twice, each time for 1 h. The two decoction liquids were combined, and after the tortoise plastron glue was melted, it was poured into the combined medicinal liquid, and then heated and concentrated into medicinal liquids containing 0.3, 0.6, and 1.2 g / mL of crude drug content, and stored in a 4°C refrigerator for later use. The medium-dose concentration was obtained by converting the human dosage and the equivalent dose of rats: the dosage of the ovulation-promoting formula for rats = the dosage of humans (calculated according to 60 kg) × conversion coefficient = 122 g·60 kg -1 ×6.3 ≈ 12.81 g·kg -1 Take 0.5 times of it, that is, 6.4 g·kg -1 Set it as the low-dose concentration, and take 2 times of it, that is, 25.62 g·kg -1 Set it as the high-dose concentration.

[0052] (2) After grinding conjugated estrogen into powder, it was dissolved by heating and stirring with distilled water to make a medicinal liquid with the required concentration (conjugated estrogen 0.02 mg / mL), and stored at 4°C for later use. The dosage of the conjugated estrogen group (0.21 mg / kg) was equivalent to the drug dosage of an adult with a clinical body weight of 60 kg, which was 2 mg.

[0053] 3 Modeling

[0054] Fifty rats were used to prepare thin endometrium models during the estrus period: ① Fast for 12 h before surgery, and anesthetize by intraperitoneal injection of 3% sodium pentobarbital (0.1 ml / 100 g); ② Fix the rats, prepare the skin, disinfect routinely, and drape the surgical field; ③ Make a longitudinal incision about 1.0 cm long at about 0.5 cm above the cervix. After separating one side of the uterus with forceps, clamp the uterine cervix end and ovarian end with vascular clips to prevent 95% ethanol from damaging the ovaries and vagina. ④ Insert the needle of a 1 mL syringe into the uterine cavity, keep it parallel to the longitudinal axis of the uterus, slowly inject absolute ethanol, keep the uterine cavity in a full state, and after retaining for 5 min (the dosage is about 0.5 ml), withdraw the absolute ethanol in the uterine cavity, fix the needle, and replace it with a syringe filled with 0.9% sodium chloride solution to rinse the uterine cavity 2-3 times, then remove the needle and syringe; treat the other side of the uterus in the same way; ⑤ Rinse the abdominal cavity with 0.9% sodium chloride solution and dry it with sterile gauze. After restoring the uterus to its anatomical position, close the abdomen layer by layer. Disinfect the surgical wound, sprinkle the powder of penicillin sodium for injection (0.5 g per rat) on the muscle layer and then suture the skin. Pay attention to keeping the rats warm before they wake up, and closely observe the state of the rats after waking up. Ten rats in the blank group were treated with the same surgical method and filled the uterine cavity with an equal amount of normal saline.

[0055] Two rats from each group were selected to take the uterus after 2-3 estrous cycles to measure the endometrial thickness and verify the success of model establishment.

[0056] 4 Intervention

[0057] Gavage for 14 days. Chinese medicine low-dose group (6.4 g / kg), Chinese medicine medium-dose group (12.81 g / kg), Chinese medicine high-dose group (25.62 g / kg), western medicine control group (0.18 mg / kg), blank group and model group (equal amount of normal saline).

[0058] 5 Observation indicators:

[0059] (1) Uterine wet weight, endometrial thickness, number of endometrial glands;

[0060] (2) Detect the expression level of LIF in uterine tissue by immunohistochemistry.

[0061] 6 Results

[0062] (1) Uterine wet weight, endometrial thickness, number of uterine glands

[0063] From Figure 1 、 Figure 2 and Figure 3It can be seen that compared with the blank group, the endometrial thickness and uterine wet weight in the model group decreased, verifying the successful establishment of the thin endometrium model. After intervention, compared with the model group, both the high-dose traditional Chinese medicine group and the western medicine group could increase the endometrial thickness, uterine wet weight, and the number of uterine glands, and the differences were statistically significant (P<0.01). There was no statistical difference between the high-dose traditional Chinese medicine group and the western medicine group, indicating that the curative effects of the high-dose traditional Chinese medicine group and the western medicine group were comparable.

[0064] (2) LIF expression level in uterine tissue

[0065] Leukemia inhibitory factor (LIF) is considered a marker of endometrial receptivity and is highly expressed in the uterus and embryonic tissues, preparing for good endometrial receptivity and allowing embryo implantation. In infertile women, the expression of LIF decreases or even disappears. Upregulating the expression of LIF can enhance endometrial receptivity. As can be seen from Figure 4 it that compared with the blank group, the LIF expression in the uterine tissue of rats in the TE model group decreased significantly and the difference was statistically significant (P<0.01); compared with the model group, the LIF in the uterine tissue of rats in each intervention group increased to varying degrees and the difference was statistically significant (P<0.01). There was no statistical difference between the high-dose traditional Chinese medicine group and the western medicine group, indicating that the two had comparable effects.

[0066] The results of this experiment showed that when comparing the means of the high-dose traditional Chinese medicine group and the western medicine group, the curative effect of the high-dose traditional Chinese medicine group in improving endometrial thickness could be increased by 5.2%, the curative effect in increasing the wet weight of the endometrium could be increased by 5.6%, and the curative effect in increasing the number of endometrial glands could be increased by 7.9%. Progesterone (Estradiol Valerate Tablets) is one of the clinical treatment drugs for thin endometrium. It is a natural exogenous estrogen preparation. After oral administration and metabolism, it becomes 17β-estradiol, which has a basically the same structure and similar physiological effects as estrogen, and can bind to the estrogen receptor in the endometrium, improve the blood circulation of the endometrium, and promote the development of the patient's endometrium, thus achieving the effect of treating thin endometrium. However, long-term supplementation of exogenous estrogen can increase the risk of endometrial cancer. Especially for endometrial cancer patients undergoing fertility-preserving treatment, the application of traditional Chinese medicine can reduce the risk factors leading to tumor recurrence, and at the same time, it can be added or subtracted according to the patient's symptoms to give personalized treatment to the patient.

[0067] Example 5 Typical case

[0068] Case: Li, female, 32 years old, chief complaint: irregular menstruation for 2 years. Hysteroscopy was performed in an external hospital in March 2021, and pathology showed: endometrioid carcinoma, grade I, focal with phosphation, focal stromal with decidual changes. Immunohistochemistry: ER (80%), PR (80%), p53 (individual cells +), PTEN (-), Ki-67 (10%), MLH-1 (+), MSH-2 (+), MSH-6 (+), PMS-2 (+). The patient had a strong desire to have children, and after being fully informed, she underwent fertility-preserving treatment and was given oral megestrol. Hysteroscopy was repeated in June 2021 and showed: consistent with the performance after conservative treatment of endometrioid carcinoma, partial relief of lesions (partial reaction area 10%, complete reaction area 90%). The hysteroscopy report of September 2021 was the same as before, and the plan was changed to LNG-IUS + leuprorelin + metformin + letrozole + atorvastatin. Hysteroscopy in December 2021 and March 2022 showed complete remission of the lesion. In May 2022, the patient plans to undergo in vitro fertilization-embryo transfer in another hospital. In December 2022, 8 eggs were retrieved. After in vitro fertilization, 2 high-quality embryos and 4 blastocysts were obtained, with a total of 4 frozen embryos. In the same month, she visited our outpatient clinic. At present: sleep is good, bowel movements are regular. The tongue is pale red with thin white fur, and the pulse is deep. Height 165cm, weight 65kg, body mass index (BMI) 23.88kg / m 2 . Ultrasound: The endometrium is of medium uneven thickness of 0.5cm. There are 6 follicles / field of view in the left ovary, and the diameter of the largest follicle is 2.2cm; there are 3 follicles / field of view in the right ovary, and the diameter of the largest follicle is 1.7cm. Anti-Mullerian hormone is 2.13ng / ml. Diagnosis: Thin endometrium, endometrial cancer after fertility-preserving treatment. Chinese medicine is given to improve the endometrial environment and increase endometrial receptivity before embryo transfer. The specific prescription is: 5g of tortoise shell glue (melted), 12g of wolfberry, 30g of dodder seed, 9g of trifoliate lanceolate, 9g of peach kernel, 12g of water safflower seed, 15g of large blood vine, 9g of Dipsacus asper, 15g of Eucommia ulmoides, and 6g of cinnamon twig. 14 pairs, one dose per day, divided into morning and evening. Ultrasound recheck in February 2023: Endometrial thickness is 0.8cm. Embryo transfer was performed in April 2023, and a son was born by cesarean section in February 2024. No tumor cells were found in the endometrial biopsy after the operation.

[0069] This case is a young patient with early endometrial cancer. After the patient's condition was completely relieved, Chinese medicine assisted pregnancy and improved the thickness of the endometrium, helping the patient to fulfill his wish to have children. Lycium barbarum, Cuscuta chinensis, Eucommia ulmoides, and Dipsacus asper nourish the kidney and replenish qi, Trigonum aviculare, Semen persicae, Fructus Polygoni Multiflori, and Caulis Sargentodoxae promote blood circulation, eliminate dampness, and dredge collaterals, Tortoise shell glue replenishes blood, and Cinnamon twig warms the meridians and dredges collaterals, thereby nourishing the kidney and activating blood circulation, improving endometrial receptivity, and improving the endometrial microenvironment, creating a suitable environment for embryo implantation.

[0070] Obviously, the above embodiments of the present invention are merely examples for clearly illustrating the present invention, rather than limitations on the implementation manners of the present invention. For those of ordinary skill in the art, other different forms of changes or modifications can be made based on the above description. It is impossible to list all the implementation manners here. Any obvious changes or modifications derived from the technical solutions of the present invention still fall within the protection scope of the present invention.

Claims

1. A Chinese medicine composition for treating thin endometrium, characterized in that: The traditional Chinese medicine composition is mainly prepared from the following raw materials: tortoise shell glue, wolfberry fruit, dodder seed, triangularis, peach kernel, polyanthus orientalis fruit, saffron vine, dipsacus root, eucommia bark and cassia twig.

2. The Chinese medicine composition according to claim 1, characterized in that: The traditional Chinese medicine composition is mainly prepared from the following raw materials in parts by weight: 5-10 parts of tortoise shell glue, 9-15 parts of wolfberry, 15-30 parts of dodder seeds, 9-15 parts of trifoliate lanceolate, 9-12 parts of peach kernel, 9-15 parts of polyanthus orientalis, 9-15 parts of saffron vine, 9-15 parts of dipsaccharum officinale, 9-15 parts of eucommia bark, and 6-9 parts of cassia twig.

3. The Chinese medicine composition according to claim 1, characterized in that The traditional Chinese medicine composition is mainly prepared from the following raw materials in parts by weight: 5 parts of tortoise shell glue, 12 parts of wolfberry, 30 parts of dodder seeds, 9 parts of triangularis, 9 parts of peach kernel, 12 parts of polyanthus orientalis, 15 parts of safflower seed, 9 parts of dipsacus, 15 parts of eucommia bark and 6 parts of cassia twig.

4. The Chinese medicine composition according to claim 1, characterized in that: The traditional Chinese medicine composition is mainly prepared from the following raw materials in parts by weight: 10 parts of tortoise shell glue, 15 parts of wolfberry, 30 parts of dodder seeds, 15 parts of triangularis, 12 parts of peach kernel, 15 parts of polyanthus orientalis, 15 parts of safflower seeds, 15 parts of safflower caulis, 15 parts of dipsaccharum officinale, 15 parts of eucommia bark, and 9 parts of cassia twig.

5. The Chinese medicine composition according to claim 1, characterized in that: The traditional Chinese medicine composition is mainly prepared from the following raw materials in parts by weight: 5 parts of tortoise shell glue, 9 parts of wolfberry fruit, 15 parts of dodder seed, 9 parts of triangularis, 9 parts of peach kernel, 9 parts of polyanthus orientalis, 9 parts of Sargentodoxa chinensis, 9 parts of Dipsacus asper, 9 parts of Eucommia ulmoides and 6 parts of cassia twig.

6. The method for preparing the Chinese medicinal composition for treating thin endometrium according to any one of claims 1 to 5, characterized in that: The preparation method comprises the following steps: Take wolfberry, dodder seed, three-edged sword, peach kernel, polyanthus orientalis, safflower seed, safflower vine, dipsaccharum officinale, eucommia bark and cassia twig according to the formula, soak in water for 30-40 minutes, boil and then simmer, filter to obtain the first filtrate, and retain the residue; Add water to the medicinal residue, boil it, then decoct it over low heat, and filter it to obtain a second filtrate; Combine the two filtrates; Dissolve the turtle shell glue and set aside; Pour the melted tortoise shell glue into the combined filtrate to obtain the product.

7. The preparation method according to claim 6, characterized in that: The amount of water added for each decoction is 10-15 times the total weight of the raw material; the decoction time for each decoction is 30-90 minutes; Preferably, the preparation method further comprises the step of concentrating the filtrate into a clear paste; Preferably, the clear paste has a relative density of 1.0-1.3 measured at 50°C-60°C.

8. A Chinese medicine preparation for treating thin endometrium, characterized in that: The Chinese medicine preparation uses the Chinese medicine composition according to any one of claims 1 to 5 or the Chinese medicine composition prepared by the preparation method according to any one of claims 6 or 7 as an active ingredient; Preferably, the traditional Chinese medicine preparation also includes pharmaceutically acceptable excipients.

9. The Chinese medicine preparation according to claim 8, characterized in that: The dosage form of the Chinese medicine preparation is decoction, granules, pills, capsules, tablets, powders or oral liquid.

10. Use of the Chinese medicine composition for treating thin endometrium according to any one of claims 1 to 5 or the Chinese medicine preparation for treating thin endometrium according to any one of claims 8 to 9 in the preparation of a medicament for treating thin endometrium.

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