Accurate clamping type multi-station embryo culture dish operation table and use method thereof

Abstract

The invention discloses a precise clamping type multi-station embryo culture dish operation table and a use method thereof, and relates to the technical field of embryos. A guide groove is formed in the inner side of a guide frame, a plurality of alignment flat grooves are formed in the top side face of the guide frame, and a plurality of spiral fasteners aligned with the alignment flat grooves are installed on the side face of the guide frame in a screwed mode. The clamping tooth piece comprises a toothed plate, two vertical plates fixed to the upper side of the side end of the toothed plate and a transverse rod fixed between the upper side ends of the two vertical plates, and a sliding piece installed at the guide groove in a sliding mode is further fixed to the side end of the toothed plate. The embryo culture dish comprises a lower ring body and an upper ring body, and a ring gap is formed between the periphery of the lower ring body and the upper part of the upper ring body. The gear ring piece comprises a ring plate which is positioned and sleeved at the position of the ring position notch; and an outer ring tooth opening matched with the side tooth opening is formed in the outer ring side of the ring plate. The culture dish is effectively prevented from being mistakenly touched and shaken, the problem that normal development of cells is affected by a high-intensity magnetic field and the like is also avoided, and a stable external environment is provided for embryonic cell culture.

Description

Technical Field

[0001] The present invention relates to the field of embryo technology, and in particular to a precision clamping multi-station embryo culture dish operating table and a use method thereof. Background Art

[0002] In the field of embryo culture, multiple embryo culture dishes are usually placed on the operating table, which brings a series of problems. When there are a large number of culture dishes, the placement is prone to confusion. During the operation, the operator is very likely to accidentally touch the embryo culture dish that has been operated. Embryonic cells are very fragile and sensitive. This accidental touch may change the growth environment of the embryonic cells in the culture dish, adversely affect the normal development of the embryonic cells, and thus reduce the success rate of embryo culture.

[0003] In order to solve the problem of placing the embryo culture dish stably on the operating table, the prior art has made various attempts. One common method is to set many grooves on the operating table that are consistent with the outer dimensions of the embryo culture dish, and directly fix the embryo culture dish in the grooves. Although this method limits the random movement of the culture dish to a certain extent, when the embryo culture dish is taken out, the culture dish swings with a large amplitude due to the limitation of the grooves. Embryonic cells are extremely sensitive to vibrations, and a large swing amplitude will have a large impact on the embryonic cells in the embryo culture dish, which is not conducive to the stable growth and development of the embryonic cells.

[0004] Another way is to set mutually attractive magnets at the bottom of the embryo culture dish and the surface of the operating table, and use magnetic force to stabilize the culture dish. However, this forced magnetic attraction method has obvious disadvantages. The magnetic field strength between the magnets is large, and it needs to act on the embryonic cells for a long time. The long-term action of strong magnetic fields may inhibit cell proliferation, hinder the normal division and growth of cells, and cause cell growth to be blocked. At the same time, strong magnetic fields may also induce oxidative stress responses in cells, destroy the redox balance in cells, produce excessive reactive oxygen species, damage cells, and induce cell apoptosis. These negative effects will seriously affect the normal development of embryos and reduce the quality and survival rate of embryos. Summary of the invention

[0005] To solve the above technical problems, the present invention is achieved through the following technical solutions:

[0006] The present invention provides a precise clamping multi-station embryo culture dish operating table, the operating table having a group of guide frames, the inner side of the guide frames being provided with guide grooves, the top side of the guide frames being provided with a plurality of alignment flat grooves, and the side of the guide frames being screwed with a plurality of spiral fasteners aligned with the positions of the alignment flat grooves.

[0007] Multiple tooth engaging members, each tooth engaging member including a tooth plate, two vertical plates fixed to the upper side of the side end of the tooth plate, and a cross bar fixed between the upper side ends of the two vertical plates. A sliding member slidably mounted at the guide groove position is also fixed to the side end of the tooth plate. Side tooth openings are provided on both sides of the tooth plate, and a pair of alignment vertical grooves are formed on the outer side surface of one of the vertical plates. Among them, the tightened screw fastener is in pressing contact with the side end surface of the sliding member.

[0008] An embryo culture dish, the embryo culture dish including a lower ring body and an upper ring body, with a ring position notch formed between the outer periphery of the lower ring body and above the upper ring body.

[0009] A tooth ring member, the tooth ring member including a ring plate positioned and sleeved at the ring position notch, and an outer ring tooth opening matching the side tooth opening is provided on the outer ring side of the ring plate.

[0010] As a preferred technical solution of the multi-station embryo culture dish operating table of the present invention: a rolling groove in the same direction as the guide groove is provided at the bottom side of the guide groove of the guide frame, and a roller structure rollingly mounted at the rolling groove position is configured at the bottom of the sliding member.

[0011] As a preferred technical solution of the multi-station embryo culture dish operating table of the present invention: a horizontal screw hole vertically penetrating the outer side surface of the guide frame is formed on the outer side surface of one of the guide frames, and the screw fastener is screwed and installed at the horizontal screw hole position.

[0012] As a preferred technical solution of the multi-station embryo culture dish operating table of the present invention: a positioning protrusion is provided on the outer ring side of the lower ring body, and a positioning notch matching the positioning protrusion is formed on the inner ring side surface of the ring plate.

[0013] As a preferred technical solution of the multi-station embryo culture dish operating table of the present invention: the alignment flat groove of the guide frame horizontally penetrates the top side surface of the guide frame, and the lowest point position of the alignment vertical groove of the vertical plate has the same horizontal height as the alignment flat groove.

[0014] As a preferred technical solution of the multi-station embryo culture dish operating table of the present invention: the bottom side surface of the sliding member is flush with the bottom side surface of the tooth plate. Let the distance between the bottom side surface of the guide groove of the guide frame and the upper surface of the operating table be H1, and the vertical height dimension of the lower ring body of the culture dish be H2, then H1 = H2.

[0015] As a preferred technical solution of the multi-station embryo culture dish operating table of the present invention: except for the roller structure and the tooth ring member, the tooth engaging members are all made of a transparent and non-toxic polymer material.

[0016] The present invention provides a method for using a precision clamping type multi-station embryo culture dish operating table, including the following contents:

[0017] S1. Fix and install a tooth engaging member at the middle position of the guide frame.

[0018] S1.1. When installing the gear member, hold the cross bar by hand, snap the sliding member into the guide groove, then move the toothed plate to the corresponding position aligned with the screw fastener, and fasten the position of the gear member with the screw fastener. Among them, when the alignment vertical groove of the vertical plate is aligned with the alignment flat groove of the guide frame, the sliding member of the gear member is aligned with the screw fastener.

[0019] S2. Place the culture dish with the toothed ring member already sleeved on the side of the gear member where no culture dish is placed. When placing, align the outer ring teeth of the toothed ring member with the side teeth of the gear member.

[0020] S3. After placing a certain number of culture dishes, install a new gear member on the other side of the culture dishes. Push the newly installed gear member tightly towards the culture dish direction. The side teeth of the newly installed gear member mesh with the toothed ring member of the culture dish, and fasten the position of the newly installed gear member with the screw fastener at the corresponding position.

[0021] S4. Repeat the operations in steps S2 and S3 until all the culture dishes to be fixed are meshed and clamped.

[0022] S5. When it is necessary to transfer liquid into the culture dish, directly inject the transferred liquid into each culture dish one by one.

[0023] S6. When it is necessary to take out the culture dish, remove the gear members one by one according to the reverse steps during loading, and then take out the culture dish.

[0024] Compared with the existing technology, the beneficial effects of the present invention are:

[0025] 1. In the present invention, the guide frame of the operating table is provided with a guide groove, an alignment flat groove and a screw fastener, which cooperate with the sliding member and the alignment vertical groove of the gear member to accurately position the position of the gear member. Moreover, through the meshing of the side teeth of the gear member with the outer ring teeth of the toothed ring member, and the cooperation of the positioning protrusion on the lower ring body of the culture dish and the positioning notch of the toothed ring member, the culture dish is ensured to be firmly clamped. During the installation process, when the alignment vertical groove of the vertical plate is aligned with the alignment flat groove of the guide frame, the screw fastener is aligned with the sliding member of the gear member and fastened, which is convenient for accurate fixation, effectively avoiding accidental contact and shaking of the culture dish, and providing a stable external environment for embryonic cell culture.

[0026] 2. Compared with the traditional groove fixing method, when taking out the culture dish in the present invention, there will be no large swing amplitude due to the limitation of the groove, reducing the impact on embryonic cells, and being more conducive to the stable growth and development of embryonic cells. Compared with the magnetic attraction fixing method, there is no strong magnetic field effect in the present invention, which will not affect the normal development of embryonic cells, such as inhibiting cell proliferation, triggering oxidative stress reactions, etc., ensuring the quality and survival rate of embryos. Description of the Drawings

[0027] Figure 1Schematic diagram of the stable placement of the embryo culture dish on the operating table in the present invention.

[0028] Figure 2 Schematic diagram of the structure of the operating table in the present invention.

[0029] Figure 3 For Figure 2 Schematic diagram of the partial enlarged structure at position A in

[0030] Figure 4 Schematic diagram of the structure when the culture dish is meshingly installed on two tooth engaging members in the present invention.

[0031] Figure 5 Bottom side upward view schematic diagram of the tooth engaging member in the present invention.

[0032] Figure 6 Schematic diagram of the separation of the tooth ring member and the culture dish in the present invention.

[0033] Figure 7 Schematic diagram of the structure when initially loading the tooth engaging member in the present invention.

[0034] Figure 8 Schematic diagram of the structure when the tooth engaging member meshingly clamps the culture dish in the present invention.

[0035] Wherein: 1 - operating table, 101 - guide frame, 102 - guide groove, 103 - rolling groove, 104 - alignment flat groove, 105 - horizontal screw hole, 106 - screw fastener; 2 - tooth engaging member, 201 - tooth plate, 2011 - side tooth opening, 202 - vertical plate, 2021 - alignment vertical groove, 203 - cross bar, 204 - sliding member, 2041 - roller structure; 3 - culture dish, 301 - lower ring body, 302 - upper ring body, 303 - ring position notch, 304 - positioning protrusion; 4 - tooth ring member, 401 - ring plate, 402 - positioning notch, 403 - outer ring tooth opening. Detailed implementation manners

[0036] In order to make the objectives, technical solutions and advantages of the present invention clearer, the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0037] Embodiment 1. The present invention designs a precise clamping type multi-station embryo culture dish operating table. Please refer to Figure 1 , Figure 4 , which includes an operating table 1, a tooth engaging member 2, a culture dish 3, and a tooth ring member 4. The specific structural configurations are as follows:

[0038] Operating table 1: It is the basic support part of the entire device. Combining Figure 1 , Figure 2 ,Figure 3 , Figure 4 , Figure 5 , on which a set of guide frames 101 are provided. The guide grooves 102 inside the guide frames 101 provide tracks for the installation and movement of the toothed part 2. The rolling grooves 103 at the bottom side of the guide grooves 102 cooperate with the roller structures 2041 at the bottom of the sliding parts 204 of the toothed part 2, making the movement of the toothed part 2 on the guide frame 101 smoother, reducing friction, and facilitating the adjustment of the position of the toothed part 2. A plurality of alignment flat grooves 104 are opened on the top side of the guide frame 101, which cooperate with the alignment vertical grooves 2021 on the vertical plates 202 of the toothed part 2 to accurately position the toothed part 2 on the guide frame 101 and ensure the installation accuracy. A plurality of screw fasteners 106 are screwed and installed on the side of the guide frame 101. At the corresponding positions of the alignment flat grooves 104, when the screw fasteners 106 are tightened, they can be in extrusion contact with the side end faces of the sliding parts 204 of the toothed part 2, thereby fixing the toothed part 2 and ensuring the stable position of the toothed part 2 during use. The horizontal screw holes 105 opened on the outer side of one of the guide frames 101 and vertically penetrating the guide grooves 102 facilitate the installation and operation of the screw fasteners 106, enabling the operator to tighten and loosen the toothed part 2 more conveniently.

[0039] Toothed part 2: Combining Figure 2 , Figure 3 , Figure 4 , is composed of a toothed plate 201, vertical plates 202, cross bars 203 and sliding parts 204. The side teeth 2011 provided on both sides of the toothed plate 201 are used to mesh with the outer ring teeth 403 of the toothed ring part 4 to realize the clamping of the embryo culture dish 3. The two vertical plates 202 fixed on the upper side of the side end of the toothed plate 201 play a role in connecting the cross bars 203 and positioning. The alignment vertical grooves 2021 on the vertical plates 202 cooperate with the alignment flat grooves 104 of the guide frame 101 for positioning. The cross bars 203 are convenient for the operator to hold and are easier to operate when installing and adjusting the toothed part 2. The sliding parts 204 fixed to the side end of the toothed plate 201 are installed in the guide grooves 102 of the guide frame 101, and the roller structures 2041 at their bottoms roll in the rolling grooves 103, enabling the toothed part 2 to move flexibly. Except for the roller structures 2041, the toothed part 2 is made of a transparent and non-toxic polymer material to avoid contamination and adverse effects on embryonic cells and ensure the safety of the embryonic culture environment.

[0040] Embryo culture dish 3: Combining Figure 1 , Figure 4 , Figure 6, which is composed of a lower ring body 301 and an upper ring body 302. A ring position notch 303 is formed between the outer periphery of the lower ring body 301 and above the upper ring body 302 for installing the toothed ring part 4, so that the toothed ring part 4 can be stably sleeved on the embryo culture dish 3. A positioning protrusion 304 is arranged on the outer ring side of the lower ring body 301 and cooperates with the positioning notch 402 on the inner ring side surface of the toothed ring part 4 to further enhance the installation stability of the embryo culture dish 3 and the toothed ring part 4, prevent the toothed ring part 4 from rotating or shifting on the culture dish 3, and ensure that the position of the culture dish 3 is fixed accurately during the clamping process.

[0041] Toothed ring part 4: Combined with Figure 1 , Figure 4 , Figure 6 , it includes a ring plate 401 positioned and sleeved at the position of the ring position notch 303 of the embryo culture dish 3. The outer ring tooth opening 403 arranged on the outer ring side of the ring plate 401 cooperates with the side tooth opening 2011 of the tooth engaging part 2, and the embryo culture dish 3 is clamped and fixed through the engagement of the tooth openings. The positioning notch 402 on the inner ring side surface of the ring plate 401 cooperates with the positioning protrusion 304 of the lower ring body 301 of the embryo culture dish 3 to improve the installation accuracy and stability, and ensure that the culture dish 3 will not shake randomly during the operation.

[0042] Embodiment 2: The present invention designs an operation and use method for precise clamping of a multi-station embryo culture dish operation table. The specific operation and use process is as follows:

[0043] Step 1, initial installation of the tooth engaging part: As Figure 7 , a tooth engaging part 2 is fixedly installed at the middle position of the guide frame 101. During installation, the operator holds the cross bar 203 by hand, inserts the sliding part 204 into the guide groove 102, and then moves the tooth plate 201 to align the alignment vertical groove 2021 of the vertical plate 202 with the alignment flat groove 104 of the guide frame 101. At this time, the sliding part 204 of the tooth engaging part 2 is aligned with the screw fastener 106, and then the screw fastener 106 is tightened to firmly fix the tooth engaging part 2 on the guide frame 101.

[0044] Step 2, placing the culture dish: Combined with Figure 7 , Figure 8 , on the side of the installed tooth engaging part 2 where no culture dish is placed, place the embryo culture dish 3 sleeved with the toothed ring part 4.

[0045] When placing, carefully engage and align the outer ring tooth opening 403 of the toothed ring part 4 with the side tooth opening 2011 of the tooth engaging part 2 to ensure that the tooth openings are completely fitted, so that the culture dish 3 is initially fixed on the tooth engaging part 2.

[0046] Step 3, installation of the subsequent tooth engaging part and clamping of the culture dish: Combined with Figure 8 , after placing a certain number of culture dishes 3, a new tooth engaging part 2 is installed on the other side of the culture dish 3.

[0047] During installation, first snap the sliding member 204 of the new tooth member 2 into the guide groove 102, and push it tightly towards the direction of the culture dish 3, so that the side tooth opening 2011 of the new tooth member 2 meshes with the outer ring tooth opening 403 of the tooth ring member 4 on the culture dish 3. When the number of the outer ring tooth openings 403 of the tooth ring member 4 is even, due to its symmetric distribution characteristics, it is easier to complete the meshing with the side tooth opening 2011 of the newly installed tooth member 2. After the meshing is completed, fasten the newly installed tooth member 2 with the screw fastener 106 at the corresponding position to make it stably fixed and further clamp the culture dish 3.

[0048] Step Four, cyclic operation: Repeat the actions of Step Two and Step Three, continuously install the tooth member 2 and place the culture dish 3 until all the embryo culture dishes 3 to be fixed are meshed and clamped as required, so as to achieve the precise fixation of multiple-station culture dishes on the operating table.

[0049] Step Five, pipetting operation: When pipetting into the culture dish 3 is required, the operator can directly inject the pipetting liquid into each culture dish 3 one by one. Since the culture dish 3 has been precisely fixed, there will be no shaking, displacement, etc. during the pipetting process, ensuring the accuracy and stability of the pipetting operation and avoiding unnecessary interference to the embryonic cells.

[0050] Step Six, removing the culture dish: When the culture dish 3 needs to be removed, operate according to the reverse steps during loading. First, loosen the screw fasteners 106 fixing the tooth member 2 one by one, then remove the tooth member 2 from the guide frame 101, and finally take out the embryo culture dish 3 from the operating table. The whole process is orderly and will not damage the embryonic cells.

[0051] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A precision clamping multi-station embryo culture dish operating table, characterized in that: include: An operating table (1), wherein a group of guide frames (101) are provided on the operating table (1), a guide groove (102) is provided inside the guide frame (101), a plurality of alignment flat grooves (104) are provided on the top side surface of the guide frame (101), and a plurality of spiral fasteners (106) aligned with the alignment flat grooves (104) are screwed and installed on the side surface of the guide frame (101); A plurality of latching tooth members (2), the latching tooth members (2) comprising a tooth plate (201), two vertical plates (202) fixed to the upper side of the side end of the tooth plate (201), and a cross bar (203) fixed between the upper side ends of the two vertical plates (202); a sliding member (204) slidably mounted at the position of the guide groove (102) is also fixed to the side end of the tooth plate (201); side tooth openings (2011) are provided on both sides of the tooth plate (201); and an alignment vertical groove (2021) is provided on the outer side surface of one of the vertical plates (202); The tightened spiral fastener (106) is in extrusion contact with the side end surface of the sliding member (204); An embryo culture dish (3), the embryo culture dish (3) comprising a lower ring body (301) and an upper ring body (302), wherein a ring position notch (303) is formed between the periphery of the lower ring body (301) and the upper part of the upper ring body (302); The toothed ring component (4) comprises a ring plate (401) which is positioned and sleeved at the position of the ring position notch (303); the outer ring side of the ring plate (401) is provided with an outer ring tooth opening (403) which matches the side tooth opening (2011).

2. The precise clamping multi-station embryo culture dish operating table according to claim 1, characterized in that: The bottom side of the guide groove (102) of the guide frame (101) is provided with a rolling groove (103) in the same direction as the guide groove (102), and the bottom of the sliding member (204) is provided with a roller structure (2041) rollingly installed at the position of the rolling groove (103).

3. The precise clamping multi-station embryo culture dish operating table according to claim 1, characterized in that: A horizontal screw hole (105) vertically penetrating the guide groove (102) is provided on the outer side surface of one of the guide frames (101), and the screw fastener (106) is screwed and installed at the position of the horizontal screw hole (105).

4. The precise clamping multi-station embryo culture dish operating table according to claim 1, characterized in that: The outer ring side of the lower ring body (301) is provided with a positioning protrusion (304), and the inner ring side of the ring plate (401) is provided with a positioning notch (402) that matches the positioning protrusion (304).

5. The precise clamping multi-station embryo culture dish operating table according to claim 1, characterized in that: The alignment flat groove (104) of the guide frame (101) extends transversely through the top side of the guide frame (101), and the lowest point of the alignment vertical groove (2021) of the vertical plate (202) is at the same level as the alignment flat groove (104).

6. The precise clamping multi-station embryo culture dish operation table according to claim 1, characterized in that: The bottom side of the sliding member (204) is flush with the bottom side of the toothed plate (201), and the distance between the bottom side of the guide groove (102) of the guide frame (101) and the upper surface of the operating table is assumed to be H1, and the vertical height dimension of the lower ring body (301) of the culture dish (3) is H2, then H1=H2.

7. The precise clamping multi-station embryo culture dish operating table according to claim 1, characterized in that: The latching tooth component (2), except for the roller structure (2041) and the tooth ring component (4), are all made of transparent, non-toxic polymer materials.

8. A method for using a precision clamping multi-station embryo culture dish operating table, characterized in that: A precision clamping multi-station embryo culture dish operating table according to any one of claims 1 to 7, comprising the following contents: S1. A toothed member (2) is fixedly mounted at a middle position of the guide frame (101); S1.

1. When installing the latching tooth member (2), hold the crossbar (203) by hand, insert the sliding member (204) into the guide groove (102), then move the tooth plate (201) to a corresponding position aligned with the spiral fastener (106), and fasten the position of the latching tooth member (2) by the spiral fastener (106); When the alignment vertical groove (2021) of the vertical plate (202) is aligned with the alignment flat groove (104) of the guide frame (101), the sliding member (204) of the latching member (2) is aligned with the spiral fastener (106); S2. Place the culture dish (3) with the gear ring member (4) mounted on the side where the culture dish (3) is not placed on the card tooth member (2). When placing the culture dish (3), align the outer ring teeth (403) of the gear ring member (4) with the side teeth (2011) of the card tooth member (2); S3. After placing a certain number of culture dishes (3), a new latching tooth member (2) is installed on the other side of the culture dish (3), and the newly installed latching tooth member (2) is pushed toward the culture dish (3), and the side teeth (2011) of the newly installed latching tooth member (2) are engaged with the toothed ring member (4) of the culture dish (3), and the newly installed latching tooth member (2) is fastened by the spiral fastener (106) at the corresponding position; S4. Repeat the steps S2 and S3 until all the culture dishes (3) that need to be fixed are engaged and clamped; S5. When it is necessary to transfer liquid into the culture dish (3), directly inject the transfer liquid into the culture dish (3) one by one; S6. When the culture dish (3) needs to be taken out, the latching members (2) are removed one by one in the reverse order of the loading process, and the culture dish (3) is taken out.

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