Cuora flavomarginata sperm collecting device and artificial insemination method

Abstract

The invention discloses a cuora flavomarginata sperm collecting device and an artificial insemination method. The cuora flavomarginata sperm collecting device comprises a box body, a fixing mechanism, a spraying system, a female turtle tail simulation part and a sperm receiving groove. A supporting inclined plate for placing the cuora flavomarginata is arranged in the box body; the fixing mechanism is configured to fix the cuora flavomarginata; the spraying system is configured to spray the turtle back of the cuora flavomarginata; the position of the female turtle tail simulation part corresponds to the position of the tail part of the cuora flavomarginata fixed on the supporting inclined plate; the semen receiving groove is used for collecting semen discharged by the cuora flavomarginata. According to the artificial insemination method for the cuora flavomarginata, artificial insemination of the cuora flavomarginata can be achieved, the artificial insemination technology for the cuora flavomarginata achieves breakthrough from nothing to nothing, the breeding potential of the breeding cuora flavomarginata is brought into full play, and the problems that the cuora flavomarginata is uneven in mating resource distribution, difficult to mate and not fertilized in the artificial feeding environment are fundamentally solved.

Description

Technical Field

[0001] The present invention relates to the technical field of Cuora flavomarginata breeding, and particularly relates to a sperm extraction device for Cuora flavomarginata and an artificial insemination method. Background Art

[0002] Cuora flavomarginata is a characteristic native species in China. Due to human capture and habitat destruction, the wild resources of Cuora flavomarginata are increasingly depleted and it has been included in the national second-class protected animals (only wild populations). Cuora flavomarginata is gentle and timid, easy to be stressed, with low aggressiveness towards humans, but has strong vigilance and will quickly shrink into its shell or flee when frightened. Male adult turtles have strong territoriality and may fight for mates during the breeding period (such as biting each other and hitting the carapace).

[0003] In recent years, with the gradual popularization of the ornamental and medicinal values of Cuora flavomarginata, the scale of its artificial breeding industry has gradually expanded, and the artificial breeding technology for Cuora flavomarginata has been relatively mature.

[0004] Currently, the main research direction for Cuora flavomarginata breeding is to improve the hatching rate of Cuora flavomarginata by regulating factors such as its diet, breeding environment, and hatching conditions. However, regulating these factors cannot solve the problems of difficult mating of breeding turtles and low fertilization rate.

[0005] The natural mating method of Cuora flavomarginata is that when male and female turtles are in heat at the same time, the male turtle climbs onto the back of the female turtle, and its genital organ extends from the cloaca and probes into the female turtle's cloaca to discharge semen, thus completing the entire fertilization process. In the artificial breeding environment, affected by factors such as single diet, small temperature difference, fighting among male turtles for mates, few female turtles in heat and not cooperating, and waste of mating opportunities due to multiple matings of some female turtles, the results of natural mating are often not ideal. The fertilization rate of female turtles is usually between 50% - 80%, the fertilization rate is relatively low, and even the situation of non-fertilization occurs.

[0006] Mastering the artificial insemination technology for Cuora flavomarginata is a major breakthrough in the development of the Cuora flavomarginata industry. Its significance lies in improving the output by giving full play to the reproductive potential of breeding turtles and contributing to the development of the Cuora flavomarginata seed industry. However, there is currently no relevant report on the artificial insemination technology for Cuora flavomarginata. Summary of the Invention

[0007] To solve the above technical problems, the present application provides a sperm extraction device for Cuora flavomarginata and an artificial insemination method, which can achieve a single sperm extraction success rate of 45%, realizing a breakthrough from scratch in the artificial insemination technology for Cuora flavomarginata, giving full play to the reproductive potential of breeding turtles, and fundamentally reducing the problems of uneven distribution of mating resources, difficult mating, and non-fertilization existing in the artificial breeding environment of Cuora flavomarginata.

[0008] A sperm extraction device for Cuora flavomarginata includes: Box body; a support inclined plate for placing the yellow-margined box turtle is arranged inside the box body; the support inclined plate includes a high end for placing the tail of the yellow-margined box turtle and a low end for placing the head of the yellow-margined box turtle; the high end is flush with the upper edge of the box body; Fixing mechanism; the fixing mechanism is fixedly connected with the box body and is configured to fix the yellow-margined box turtle placed on the support inclined plate; Spray system; the spray system includes a nozzle; the nozzle is configured to spray the back of the yellow-margined box turtle fixed on the support inclined plate; Female turtle tail simulation part; the female turtle tail simulation part is fixedly arranged on the outer wall of the box body on the side for placing the tail of the yellow-margined box turtle; the position of the female turtle tail simulation part corresponds to the position of the tail of the yellow-margined box turtle fixed on the support inclined plate; Sperm receiving groove; the sperm receiving groove is arranged below the female turtle tail simulation part and is used for collecting the semen discharged by the yellow-margined box turtle.

[0009] Preferably, the female turtle tail simulation part includes a column part and an inclined cone part; one end of the column part is fixedly connected with the outer wall of the box body, and the other end of the column part is fixedly connected with the head end of the inclined cone part; the tail end of the inclined cone part is higher than the highest point of the column part.

[0010] Preferably, it further includes a fluid guiding body for guiding the semen discharged by the yellow-margined box turtle; the fluid guiding body is fixedly connected to the lower edge of the column part.

[0011] Preferably, the fixing mechanism includes 4 fixing parts symmetrically arranged on both sides of the box body; the fixing part includes a fixing seat, a fixing rod, a knob and a fixing plate; the fixing seat is fixedly connected with the outer wall of the box body; the fixing rod includes a first fixing rod piece and a second fixing rod piece which are non-collinear; one end of the first fixing rod piece is hinged with the fixing seat, and the other end of the first fixing rod piece is fixedly connected with the second fixing rod piece; the fixing plate is fixedly arranged at the end head of the second fixing rod piece far away from the connection end with the first fixing rod piece; the knob is used to fasten the first fixing rod piece and the fixing seat; an anti-slip pad is fixedly arranged on the surface of the fixing plate.

[0012] Preferably, the surface of the support inclined plate is an anti-slip surface.

[0013] Preferably, the sperm receiving groove is fixedly connected with the outer wall of the box body; the sperm receiving groove includes a groove body with a semi-cone groove structure.

[0014] According to another aspect of the present application, a method for artificial insemination of the yellow-margined box turtle is further provided, which uses the sperm extraction device of the yellow-margined box turtle for sperm extraction, including: Select healthy and mature breeding turtles that have been acclimated in an artificial environment for more than half a year and are over 8 years old. During the breeding season, use the sperm extraction device for Cuora flavomarginata to extract sperm from male turtles. Within half an hour after sperm extraction, use the obtained semen of male turtles to perform artificial insemination on female turtles.

[0015] Preferably, select healthy and mature breeding turtles that have been acclimated in an artificial environment for more than half a year and are over 8 years old. During the breeding season, use the sperm extraction device for Cuora flavomarginata to extract sperm from male turtles, including: Select healthy and mature male turtles that have been acclimated in an artificial environment for more than half a year and are over 8 years old. Feed them normally to ensure that the male turtles have good physical strength and avoid stress. Three days before sperm extraction, stop feeding the male turtles. One day before sperm extraction, soak them in water to clean their intestines, and place them in a dark place in the sperm extraction environment for separate rest. During sperm extraction, clean the sperm extraction device for Cuora flavomarginata. After the device is dry, place the male turtle to be sperm-extracted on the support inclined plate, so that the end of the caudal scute of the male turtle should extend 1-2 cm beyond the edge of the support inclined plate, and use the fixing mechanism to fix the male turtle. The operator leaves the sperm extraction room to allow Cuora flavomarginata to adapt to the environment of the sperm extraction room and reduce the tension of the male turtle. Use a nozzle to spray the back of Cuora flavomarginata fixed on the support inclined plate to stimulate the male turtle to enter the breeding state. When the head of the male turtle stretches back and forth, its nostrils spray water or white foam, and its genitalia begin to swell, turn off the spraying system and get ready for sperm extraction. As soon as it is observed by the visual monitoring device that the genitalia have swollen to the maximum and then begin to contract and semen flows out, quickly enter the sperm extraction room and collect the semen in the sperm collection trough. After the genitalia of the male turtle have completely retracted, remove the male turtle to complete the sperm extraction process.

[0016] Preferably, when using a nozzle to spray the back of Cuora flavomarginata fixed on the support inclined plate, use pure water that is 3 degrees lower than the room temperature of the sperm extraction room for spraying.

[0017] Preferably, within half an hour after sperm extraction, use the obtained semen of male turtles to perform artificial insemination on female turtles, including: Half an hour before sperm extraction of male turtles, soak the female turtles in water to clean their intestines, dry their bodies, and disinfect the surrounding area of the cloaca with povidone iodine solution. Within half an hour after sperm extraction, place the female turtle upside down with its ventral carapace facing up, hold its tail, insert a syringe without a needle containing semen into its cloaca about 1 cm, and push the piston of the syringe to inject the semen into the cloaca. Remove the syringe, pinch the tail of the female turtle to close the cloaca. Keep the female turtle upside down for about five minutes, and use the contraction and expansion of the cloaca of the female turtle to make the semen flow into the fallopian tubes to complete artificial insemination.

[0018] Compared with the prior art, the present application has at least the following beneficial effects: 1. The present invention can achieve artificial sperm extraction of Cuora flavomarginata, which makes a breakthrough from scratch in the artificial insemination technology of Cuora flavomarginata, gives full play to the reproductive potential of breeding turtles, and fundamentally reduces the problems of uneven distribution of mating resources, difficult mating, and infertility existing in the artificial breeding environment of Cuora flavomarginata.

[0019] 2. The sperm extraction device for Cuora flavomarginata of the present invention has a female turtle tail simulation part for simulating the tail of a female turtle, so that the male turtle's genital organ can smoothly protrude after hooking the female turtle tail simulation part. Otherwise, even if the male turtle is in heat, it rarely protrudes its genital organ to ejaculate, resulting in sperm extraction failure.

[0020] 3. The sperm extraction device for Cuora flavomarginata of the present invention adopts a slope design with a support inclined plate, so that the water will not spill into the sperm extraction tank while the turtle is taking a shower and soaking in water, avoiding the failure of sperm extraction caused by the mixing of spray water and semen. At the same time, through the setting of the fluid guide body, the success rate of semen collection is improved.

[0021] 4. When artificial insemination is carried out by using the artificial insemination method of the present invention, the fertilization rate of female turtles inseminated twice exceeds 95%. Each male turtle can extract sperm more than 30 times a year, and the success rate of single sperm extraction is 45%, that is, the sperm extraction is successful more than 13 times, which can make more than 6 female turtles reach a fertilization rate of more than 95%, greatly improving the production efficiency.

[0022] 5. The artificial insemination method for Cuora flavomarginata of the present invention adopts a remote monitoring and control method during sperm extraction, effectively avoiding the problem of low sense of security of male turtles when they are in heat, and improving the success rate of sperm extraction. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Some specific embodiments of the present invention will be described in detail hereinafter with reference to the accompanying drawings in an exemplary but non-limiting manner. The same reference numerals in the drawings denote the same or similar components or parts. Those skilled in the art should understand that these drawings are not necessarily drawn to scale. In the drawings: Figure 1 is a schematic diagram of the overall structure of the sperm extraction device for Cuora flavomarginata of the present invention; Figure 2 is a schematic diagram of the structure of the female turtle tail simulation part of the present invention; Figure 3 is a schematic diagram of the structure of the fixing rod of the present invention; Figure 4 is a schematic diagram of the overall process of the artificial insemination method for Cuora flavomarginata of the present invention.

[0024] Among them, the above-mentioned drawings include the following reference numerals: 1. Box body; 2. Support inclined plate; 3. Fixing member; 4. Spraying system; 5. Female turtle tail simulation part; 6. Sperm receiving groove; 7. Fluid guiding body; 8. Drainage groove; 31. Fixed seat; 32. Fixed rod; 33. Knob; 34. Fixed plate; 41. Support frame; 42. Rigid water pipe; 43. Nozzle; 51. Cylindrical part; 52. Oblique conical part; 321. First fixed rod member; 322. Second fixed rod member. Detailed implementation manners

[0025] To make the objectives, technical solutions and advantages of this application clearer, the technical solutions of this application will be clearly and completely described below in conjunction with the specific embodiments of this application and the corresponding drawings. Obviously, the described embodiments are only a part of the embodiments of this application, rather than all the embodiments. Based on the embodiments in this application, all other embodiments obtained by those of ordinary skill in the art without making creative efforts shall fall within the protection scope of this application.

[0026] Based on the fact that the yellow-margined box turtle is gentle, timid, easily stressed, has low aggressiveness towards humans, but has strong vigilance and will quickly retract into its shell or flee when frightened. Male adult turtles have strong territoriality and may fight for mates during the breeding season (such as biting each other and hitting the carapace). At the same time, the genital organ of the male yellow-margined box turtle is located in the cloaca and is usually hidden inside the body. It protrudes out by congesting and erecting and turning outwards during mating. Therefore, the difficulty in artificial insemination of the yellow-margined box turtle lies in sperm extraction from the male turtle.

[0027] This solution can achieve a single sperm extraction success rate of 45% by setting up a sperm extraction device for the yellow-margined box turtle, using the sperm extraction device for sperm extraction, and relying on the artificial insemination method for the yellow-margined box turtle. It has achieved a breakthrough from scratch in the artificial insemination technology of the yellow-margined box turtle, giving full play to the breeding potential of breeding turtles, and fundamentally reducing the problems of uneven distribution of mating resources, difficult mating, and infertility existing in the artificial breeding environment of the yellow-margined box turtle.

[0028] As Figures 1 - 3 shown, a sperm extraction device for the yellow-margined box turtle includes: a box body 1, a fixing mechanism, a spraying system 4, a female turtle tail simulation part 5, and a sperm receiving groove 6.

[0029] Inside the box body 1, there is a support inclined plate 2 for placing the yellow-margined box turtle. The support inclined plate 2 includes a high end for placing the tail of the yellow-margined box turtle and a low end for placing the head of the yellow-margined box turtle. Among them, the high end is flush with the upper edge of the box body 1. Preferably, the box body 1 is 20 cm long, 15 cm wide, and 5 cm high. It should be noted that the box body 1 can be of other sizes as long as it can accommodate the yellow-margined box turtle.

[0030] The fixing mechanism is fixedly connected to the box body 1 and is configured to fix the yellow-margined box turtle placed on the support inclined plate 2.

[0031] The spray system 4 includes a nozzle 43 , which is configured to spray the back of the yellow-margined box turtle fixed on the supporting inclined plate 2 .

[0032] The female turtle tail simulation part 5 is fixedly arranged on the outer wall of the box body 1 on one side of the tail of the yellow-margined box turtle. The position of the female turtle tail simulation part 5 corresponds to the position of the tail of the yellow-margined box turtle fixed on the support inclined plate 2, so that when the yellow-margined box turtle is fixed on the support inclined plate 2 and stimulated, the tail tip of the female turtle can be hooked on the female turtle tail model and the genitals can be turned out. On the contrary, after many tests, it was found that without this female turtle tail simulation part 5, even if the male turtle is in estrus, it rarely sticks out its genitals to ejaculate, resulting in failure of sperm collection.

[0033] The semen receiving groove 6 is arranged below the female turtle tail simulation part 5 and is used for collecting the semen discharged by the yellow-margined box turtle.

[0034] In this embodiment, the female turtle tail simulation part 5 includes a column 51 and an oblique cone 52; one end of the column 51 is fixedly connected to the outer wall of the box 1, and the other end of the column 51 is fixedly connected to the head end of the oblique cone 52. The tail end of the oblique cone 52 is higher than the highest point of the column 51, so that the oblique cone 52 is in a tilted state, which is convenient for the male turtle tail tip to hook. Preferably, the angle between the axis of the oblique cone 52 and the axis of the column 51 is 30 degrees, and the tail end of the oblique cone 52 is smoothly transitioned.

[0035] Furthermore, the length of the female turtle tail simulation piece is 3 cm, wherein the diameter of the column portion 51 is 1 cm.

[0036] As another embodiment of the present invention, a semen collection device for a yellow-margined box turtle also includes a drainage body 7 for draining semen discharged by the yellow-margined box turtle. The drainage body 7 is fixedly connected to the lower edge of the column portion 51, and can drain the semen on the female turtle's tail simulation piece 5, thereby improving the semen collection effect and reducing the adhesion of semen on the female turtle's tail simulation piece 5.

[0037] Preferably, the drainage body 7 is in the shape of an inverted cone. After the male turtle discharges semen, the semen will gather or fall at the lower end of the drainage body 7, thereby improving the diversion effect.

[0038] In this embodiment, the fixing mechanism includes four fixing members 3 symmetrically arranged on both sides of the box body 1. The four fixing members 3 fix the box turtle shell and cooperate with the supporting inclined plate 2 to fix the box turtle to be sperm-extracted. The fixing member 3 includes a fixing base 31, a fixing rod 32, a knob 33 and a fixing plate 34. The fixing base 31 is fixedly connected to the outer wall of the box body 1. The fixing rod 32 includes a first fixing rod member 321 and a second fixing rod member 322 which are non-collinearly arranged. The non-collinearly arranged first fixing rod member 321 and second fixing rod member 322 have a certain included angle, so that after being hinged to the box body 1, the fixing rod 32 has a better fixing angle. One end of the first fixing rod member 321 is hinged to the fixing base 31, and the other end of the first fixing rod member 321 is fixedly connected to the second fixing rod member 322. The fixing plate 34 is fixedly arranged at the end head of the second fixing rod member 322 far from the connection end with the first fixing rod member 321. Among them, the knob 33 is used to fasten the first fixing rod member 321 to the fixing base 31. The surface of the fixing plate 34 is fixedly provided with an anti-slip pad to increase the friction with the surface of the box turtle shell and further prevent the box turtle from escaping during the sperm extraction process.

[0039] As another embodiment of the present invention, the sperm receiving groove 6 is fixedly connected to the outer wall of the box body 1. The sperm receiving groove 6 includes a groove body with a semi-conical groove structure. Since the amount of semen discharged by the box turtle each time is small, the groove body with a semi-conical groove structure can make the semen discharged by the box turtle gather inside the sperm receiving groove 6, facilitating semen collection.

[0040] As another embodiment of the present invention, the spraying system 4 includes a support frame 41, a rigid water pipe 42, a nozzle 43, as well as a water pump and a water tank. The nozzle 43 is fixed below the rigid water pipe 42, and the spray outlet of the nozzle 43 is arranged obliquely downward, so that the nozzle 43 can spray water in a certain inclination angle towards the head direction of the box turtle and make the sprayed water fall on the turtle shell. While spraying and stimulating the box turtle, it can avoid spraying into the sperm receiving groove 6.

[0041] In addition, the nozzle 43 is connected to the water tank through the rigid water pipe 42 and other pipelines communicating with the rigid water pipe 42. The water pump is arranged on the pipeline between the nozzle 43 and the water tank to provide the power for pumping water.

[0042] Preferably, the support frame 41 has a cavity inside, and the water pump and the water tank are located in this cavity. This structure can effectively reduce the space occupied by the spraying system 4 and improve the integration degree.

[0043] In this embodiment, the nozzle 43 faces the front half of the box turtle, the spraying speed is controlled at 1.5 L / h, and the spraying system can be remotely controlled through the control system.

[0044] As another embodiment of the present invention, the surface of the support inclined plate 2 is a non-slip surface. When the surface of the support inclined plate 2 is a non-slip surface, the friction between the bottom plate of the turtle and the surface of the support inclined plate 2 can be increased, and a fulcrum is provided for the front paws of the turtle, thereby increasing the sense of security of the male turtle.

[0045] As another embodiment of the present invention, a drainage groove 8 is opened on the side wall of the box body 1 connected to the lower end of the supporting inclined plate 2. The drainage groove 8 is arranged above the lower end of the supporting inclined plate 2 and penetrates the side wall of the box body 1, so that the accumulated water in the box body 1 can be discharged in time to prevent the yellow-margined box turtle from drowning.

[0046] Based on the same inventive concept, Figure 4 As shown, the present invention also provides a method for artificial insemination of a yellow-margined box turtle, which uses a yellow-margined box turtle sperm collection device to collect sperm, and includes the following steps: S1. Select healthy and mature breeding turtles that have been raised in an artificial environment for more than half a year and are over 8 years old. Use the yellow-margined box turtle semen collection device to collect semen from male turtles during the estrus period.

[0047] Specifically, select healthy and mature male turtles that have been raised in an artificial environment for more than half a year and are over 8 years old. Feed them normally to ensure that the male turtles are in good physical condition and avoid stress.

[0048] When selecting breeding turtles, the weight of male turtles should not be less than 0.35kg (the size range of mature male turtles is 14-19cm in length, 8-14cm in width, and 5-10cm in height), and the weight of female turtles should not be less than 0.5kg. The selected breeding turtles should be raised in an artificial environment for more than half a year and fed normally with turtle food, vegetables, insects, etc. to ensure that the breeding turtles are in good physical strength and avoid stress.

[0049] In addition, it is recommended to extract semen from male turtles during the estrus period from March to April and August to October each year to increase the success rate of semen extraction.

[0050] Stop feeding the male turtle three days before sperm collection, soak it in water to clean its intestines one day before sperm collection, and place it in a dark place in the sperm collection environment to rest alone.

[0051] When collecting semen, the yellow-margined box turtle semen collection device is cleaned. After the yellow-margined box turtle semen collection device is dry, the male turtle to be collected semen is placed on the supporting inclined plate, so that the end of the male turtle's tail shield should extend 1-2 cm from the edge of the supporting inclined plate, and the male turtle is fixed using a fixing mechanism.

[0052] The operator leaves the semen collection room to allow the yellow-margined box turtle to adapt to the environment of the semen collection room and reduce the tension of the male turtle.

[0053] The back of the yellow-margined box turtle fixed on the supporting inclined plate is sprayed with a nozzle to stimulate the male turtle to estrus. When spraying the back of the yellow-margined box turtle, pure water 3 degrees lower than the room temperature of the semen collection room is used for spraying.

[0054] When the head of the male turtle retracts back and forth, water sprays out of its nostrils or white foam ejects, turn off the spraying system when the genitalia begins to expand, and get ready for sperm collection.

[0055] When it is observed by the visual monitoring device that the genitalia has expanded to the maximum and then begins to contract with semen flowing out, enter the sperm collection room as soon as possible and collect the semen in the sperm collection trough.

[0056] After the male turtle's genitalia has completely retracted, remove the male turtle to complete the sperm collection process.

[0057] It should be noted that when collecting sperm, a camera needs to be placed near the sperm collection device of the yellow-margined box turtle to observe the reaction of the yellow-margined box turtle, and then take corresponding operation measures.

[0058] When there is no reaction after spraying for more than 20 minutes, at this time, remove the male turtle and replace it with another male turtle for sperm collection. The failed male turtle is put back into the isolation environment and can be tried again in the evening until successful.

[0059] In addition, the semen volume discharged by the male turtle each time is between 0.2 - 0.5 ml, and sperm collection should not be too frequent. It is recommended not to exceed 6 times per month.

[0060] S2. Within half an hour after sperm collection, artificially inseminate the female turtle with the obtained semen of the male turtle.

[0061] Specifically, it includes the following process: Soak the female turtle in water to clean its intestines half an hour before sperm collection of the male turtle, dry its body, and disinfect the periphery of the cloaca with povidone iodine solution.

[0062] Within half an hour after sperm collection, place the female turtle upside down with its ventral carapace facing up, hold its tail, insert a syringe without a needle containing semen into its cloaca about 1 cm, and push the piston of the syringe to inject the semen into the cloaca.

[0063] Take out the syringe, pinch the tail of the female turtle to close the cloaca.

[0064] Keep the female turtle upside down for about five minutes, and use the contraction and expansion of the female turtle's cloaca to make the semen flow into the fallopian tubes to complete artificial insemination.

[0065] It should be noted that the artificial insemination process is carried out in an indoor environment with quietness, moderate light, and a temperature between 18 - 25 degrees. Example

[0066] During the mating season (March - April, August - October), select an appropriate number of male turtles with an average weight of 0.5 kg, an average age of 15 years, normal body shape, and no injuries on the body surface. Stop feeding them three days in advance and isolate them for individual rearing in the sperm collection room. At the same time, prepare an appropriate number of stable female turtles with an average weight of 0.75 kg, an average age of 15 years, normal body shape, and no injuries on the body surface (the maximum number is 6 times that of male turtles), and rear them normally in the artificial environment.

[0067] Soak the male turtles in water for half an hour the day before sperm collection. The water temperature is at room temperature to help the male turtles excrete and clean their intestines. On the day of sperm collection, choose a relatively cool early morning to carry out artificial insemination, ensuring that the room temperature is within the range of 18 - 25 degrees. Too low or too high temperature will affect the estrus probability of male turtles. Half an hour before sperm collection, transfer the same number of female turtles as male turtles to the sperm collection room, soak them for half an hour to excrete and clean their intestines, and dry their bodies. Disinfect the periphery of the cloaca with povidone-iodine solution. At this time, start to collect sperm from the male turtles. Use a thermometer to measure the specific room temperature, and add pure water 3 degrees lower than the room temperature to the water tank. Place the male turtle on the support inclined plate, and the end of the caudal scute should extend 1 - 2 cm beyond the edge of the support inclined plate. Make the fixing plates on the 4 fixing rods closely adhere to the carapace of the turtle. After fixing, tighten the knob. At this time, people leave the sperm collection room to let the turtle adapt to the environment and reduce the tension of the male turtle. After 5 minutes, turn on the spray system to stimulate the male turtle to be in heat. At this time, observe in front of the camera. After 2 - 10 minutes, the male turtle may hook the simulated tail of the female turtle with the tip of its tail and turn out its genital organ. At the same time, it can be observed that the head of the male turtle stretches back and forth, and water may be sprayed from the nostrils or white foam may be ejected. The genital organ begins to swell. At this time, turn off the spray system and make preparations for sperm collection. When the camera observes that the genital organ swells to the maximum and then begins to contract and semen flows out, enter the room as soon as possible. After the semen flows out, it may flow to the lowest point of the drainage body or fall to the lowest point of the sperm collection tank. Use a syringe to collect the semen and immediately inseminate the female turtle. Place the female turtle upside down with the ventral carapace facing up, hold the tail, insert a syringe without a needle about 1 cm into the cloaca, push the piston to inject the semen into the cloaca, take out the syringe, pinch the tail of the female turtle to make the cloaca close, and keep it upside down for about five minutes. At this time, the cloaca will contract and expand, and the semen will flow into the fallopian tubes, and the artificial insemination is completed.

[0068] For ease of description, spatial relative terms such as "above", "over", "on the upper surface", "upper" etc. may be used herein to describe the spatial positional relationship of one device or feature to other devices or features as shown in the figures. It should be understood that the spatial relative terms are intended to encompass different orientations in use or operation in addition to the orientation depicted in the figures of the device. For example, if the device in the figures is inverted, the device described as "above" or "over" other devices or structures will then be positioned "below" or "under" other devices or structures. Thus, the exemplary term "above" can include both the orientations of "above" and "below". The device may also be positioned in other different ways (rotated 90 degrees or in other orientations), and the corresponding interpretations of the spatial relative descriptions used herein will be made accordingly.

[0069] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit the exemplary embodiments according to the present application. As used herein, unless the context clearly indicates otherwise, the singular forms are also intended to include the plural forms. In addition, it should also be understood that when the terms "comprise" and / or "include" are used in this specification, they specify the presence of features, steps, operations, devices, components and / or combinations thereof.

[0070] It should be noted that the terms "first", "second", etc. in the description, claims and drawings of the present application are used to distinguish similar objects and are not necessarily used to describe a specific order or sequence. It should be understood that such data can be interchanged under appropriate circumstances so that the embodiments of the present application described herein can be implemented in an order other than those illustrated or described herein.

[0071] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and changes. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included within the protection scope of the present invention.

Claims

1. A sperm extraction device for Cuora flavomarginata, characterized in that, Comprising: A box body (1); a support inclined plate (2) for placing the yellow-margined box turtle is arranged inside the box body (1); the support inclined plate (2) includes a high end for placing the tail of the yellow-margined box turtle and a low end for placing the head of the yellow-margined box turtle; the high end is flush with the upper edge of the box body (1); A fixing mechanism; the fixing mechanism is fixedly connected to the box body (1) and is configured to fix the yellow-margined box turtle placed on the support inclined plate (2); A spraying system (4); the spraying system (4) includes a nozzle (43); the nozzle (43) is configured to spray the back of the yellow-margined box turtle fixed on the support inclined plate (2); A female turtle tail simulation part (5); the female turtle tail simulation part (5) is fixedly arranged on the outer wall of the box body (1) on one side for placing the tail of the yellow-margined box turtle; the position of the female turtle tail simulation part (5) corresponds to the position of the tail of the yellow-margined box turtle fixed on the support inclined plate (2); A sperm receiving groove (6); the sperm receiving groove (6) is arranged below the female turtle tail simulation part (5) and is used for collecting the semen discharged by the yellow-margined box turtle.

2. The sperm extraction device for Cuora flavomarginata as described in claim 1, characterized in that, The female turtle tail simulation part (5) includes a cylindrical part (51) and an inclined cone part (52); one end of the cylindrical part (51) is fixedly connected to the outer side wall of the box body (1), and the other end of the cylindrical part (51) is fixedly connected to the head end of the inclined cone part (52); the tail end of the inclined cone part (52) is higher than the highest point of the cylindrical part (51).

3. The sperm extraction device for Cuora flavomarginata as described in claim 2, wherein, It further includes a fluid guide body (7) for guiding the semen discharged by the yellow-margined box turtle; the fluid guide body (7) is fixedly connected to the lower edge of the cylindrical part (51).

4. The sperm extraction device for Cuora flavomarginata as described in claim 1, characterized in that, The fixing mechanism includes 4 fixing parts (3) symmetrically arranged on both sides of the box body (1); the fixing part (3) includes a fixing seat (31), a fixing rod (32), a knob (33) and a fixing plate (34); the fixing seat (31) is fixedly connected to the outer wall of the box body (1); the fixing rod (32) includes a first fixing rod piece (321) and a second fixing rod piece (322) arranged non-collinearly; one end of the first fixing rod piece (321) is hinged to the fixing seat (31), and the other end of the first fixing rod piece (321) is fixedly connected to the second fixing rod piece (322); the fixing plate (34) is fixedly arranged at the end head of the second fixing rod piece (322) far from the connection end with the first fixing rod piece (321); the knob (33) is used to fasten the first fixing rod piece (321) and the fixing seat (31); an anti-slip pad is fixedly arranged on the surface of the fixing plate (34).

5. The sperm extraction device for Cuora flavomarginata as described in claim 1, characterized in that, The surface of the support inclined plate (2) is an anti-slip surface.

6. The yellow-margined box turtle sperm extraction device according to claim 1, characterized in that The sperm receiving groove (6) is fixedly connected to the outer wall of the box body (1); the sperm receiving groove (6) includes a groove body with a semi-cone groove structure.

7. An artificial insemination method for Cuora flavomarginata, characterized in that, Using the sperm extraction device for yellow-margined box turtles according to any one of claims 1-6 for sperm extraction, including: Selecting healthy and mature breeding turtles that have been raised in an artificial environment for more than half a year and are over 8 years old, and using the sperm extraction device for yellow-margined box turtles to extract sperm from male turtles during the estrus period; Within half an hour after sperm collection, artificial insemination is performed on the female turtle using the obtained semen of the male turtle.

8. The artificial insemination method of Cuora flavomarginata as claimed in claim 7, characterized in that, Healthy and mature breeding turtles that have been acclimated in an artificial environment for more than half a year and are over 8 years old are selected. During the estrus period, a sperm collection device for Cuora flavomarginata is used to collect sperm from the male turtle, including: Healthy and mature male turtles that have been acclimated in an artificial environment for more than half a year and are over 8 years old are selected, fed normally to ensure good physical strength of the male turtles and avoid stress. Three days before sperm collection from the male turtle, feeding is stopped, and the turtle is soaked in water for intestinal cleansing one day before sperm collection, and then placed alone in a dark place in the sperm collection environment for rest. During sperm collection, the sperm collection device for Cuora flavomarginata is cleaned. After the sperm collection device for Cuora flavomarginata is dried, the male turtle to be collected is placed on the support inclined plate so that the end of the caudal scute of the male turtle extends 1 - 2 cm beyond the edge of the support inclined plate, and the male turtle is fixed using the fixing mechanism. The operator leaves the sperm collection room to allow Cuora flavomarginata to adapt to the sperm collection room environment and reduce the tension of the male turtle. The back of the Cuora flavomarginata fixed on the support inclined plate is sprayed with a nozzle to stimulate the male turtle to be in heat. When the head of the male turtle retracts back and forth, water sprays from the nostrils or white foam sprays out, and the genital organ begins to expand, the spraying system is turned off to prepare for sperm collection. When it is observed by the visual monitoring device that the genital organ has expanded to the maximum and then begins to contract and semen flows out, quickly enter the sperm collection room and collect the semen in the sperm collection trough. After the genital organ of the male turtle has completely retracted, remove the male turtle to complete the sperm collection process.

9. The artificial insemination method of Cuora flavomarginata as described in claim 8, characterized in that, When spraying the back of the Cuora flavomarginata fixed on the support inclined plate with a nozzle, pure water that is 3 degrees lower than the room temperature of the sperm collection room is used for spraying.

10. The artificial insemination method of Cuora flavomarginata as claimed in claim 7, wherein, Within half an hour after sperm collection, artificial insemination is performed on the female turtle using the obtained semen of the male turtle, including: Half an hour before sperm collection from the male turtle, the female turtle is soaked in water for intestinal cleansing, dried, and the surrounding area of the cloaca is disinfected with povidone iodine solution. Within half an hour after sperm collection, the female turtle is placed upside down with the ventral carapace facing up, the tail is held, and a syringe without a needle containing semen is inserted about 1 cm into its cloaca, and the piston of the syringe is pushed to inject the semen into the cloaca. Take out the syringe, hold the tail of the female turtle to close the cloaca. Keep the female turtle upside down for about five minutes, and use the contraction and expansion of the cloaca of the female turtle to make the semen flow into the fallopian tube to complete artificial insemination.

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