An escherichia coli sampling and detection device for mouse experiments

Abstract

The present application relates to the field of microbial sampling and detection, and particularly relates to a mouse experimental E. coli sampling and detection device. The technical problem to be solved is that when sampling and detecting E. coli in the intestinal tract of a mouse, the attention of manual operation needs to be highly concentrated, and it is easy to cause physical and mental fatigue, and the intestinal tract will be damaged if a little carelessness. In addition, the existing technology cannot remove the fat tissue existing on the outer surface of the intestinal tract, thereby affecting the sampling and detection result. The technical scheme is as follows: a mouse experimental E. coli sampling and detection device, comprising a sampling box and a fixing assembly. The first clamp block and the second clamp block are respectively arranged on the left end and the right end of the intestinal tract of the mouse to fix the intestinal tract, the injection tube is used to inject sterile water into the inside of the intestinal tract to flush, the cleaning of the intestinal tract of the mouse is completed, the pressure of manual operation is reduced, and the accidental damage of the intestinal tract of the mouse caused by manual operation is reduced.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of microbial sampling and detection, and particularly relates to an Escherichia coli sampling and detection device for mouse experiments. BACKGROUND

[0002] When sampling and detecting Escherichia coli in the intestines of a mouse, the entire intestines of the mouse are first taken out under a sterile condition, and then the intestines are washed with sterile water. However, because the intestines of the mouse are small, the operator needs to pay high attention during manual operation, and it is easy to cause physical and mental fatigue, and the intestines are easily damaged due to slight carelessness, which affects the sampling and detection result.

[0003] Meanwhile, fat tissue usually exists on the outer surface of the intestines, and the fat tissue that is not removed will affect the accuracy of the sampling result when the intestines are sampled and detected. The prior art cannot remove the fat tissue that exists on the outer surface of the intestines, thereby causing the sampling and detection result to be inaccurate. SUMMARY

[0004] In order to overcome the shortcomings that the operator needs to pay high attention during manual operation when sampling and detecting Escherichia coli in the intestines of a mouse, it is easy to cause physical and mental fatigue, and the intestines are easily damaged due to slight carelessness, and the prior art cannot remove the fat tissue that exists on the outer surface of the intestines, thereby affecting the sampling and detection result, the present application provides an Escherichia coli sampling and detection device for mouse experiments.

[0005] The technical scheme is as follows: an Escherichia coli sampling and detection device for mouse experiments, comprising a sampling box, further comprising a fixing assembly, a placing block, a first clamping block, a second clamping block, a syringe and a scraping system, the fixing assembly is installed in the sampling box, the placing block for placing the intestines of a mouse is arranged in the sampling box, a placing groove is arranged on the placing block, the first clamping block for fixing the left end of the intestines of a mouse is slidably connected to the left side of the sampling box, the second clamping block for fixing the right end of the intestines of a mouse is slidably connected to the right side of the sampling box, the syringe for flushing the inside of the intestines of a mouse is installed on the left side of the sampling box, and the syringe is arranged as a tapered tube placed horizontally to the right, and the scraping system for scraping the fat tissue on the outer surface of the intestines of a mouse is arranged on the placing block.

[0006] Preferably, the fixing assembly comprises a fixing plate, a first fixing rod and a second fixing rod, the fixing plate is fixedly connected to the middle of the sampling box, the lower side of the placing block is fixedly connected to the fixing plate, the first fixing rod is slidably connected to the left side of the sampling box, and the first clamping block is fixedly connected to the first fixing rod, the second fixing rod is slidably connected to the right side of the sampling box, and the second clamping block is fixedly connected to the second fixing rod.

[0007] Preferably, the sampling box further comprises a pressing rod, the pressing rod for pressing the contents in the intestines of a mouse is slidably connected to the left side of the sampling box, and the pressing rod is located in the syringe.

[0008] As preferred, the fixed disc, the arc-shaped rod and the limiting ring are further included; the fixed disc is slidably connected to the right side of the extruding rod; a plurality of round rods for limiting the movement of the fixed disc are fixedly connected to the fixed disc; a plurality of arc-shaped rods are fixedly connected to the fixed disc, and each arc-shaped rod is made of a deformable material; the plurality of arc-shaped rods are located in the injection tube; the limiting ring is fixedly connected to the right side of the extruding rod and located to the right of the fixed disc.

[0009] As preferred, the scraping system includes the connecting block and the arc-shaped pieces; a plurality of sliding grooves are arranged on the placing block, and the placing groove is located between the plurality of sliding grooves; the connecting block is slidably connected between the plurality of sliding grooves; the connecting block movably connects a plurality of arc-shaped pieces, and the plurality of arc-shaped pieces are rotatably connected; each arc-shaped piece is arranged in a downward convex arc shape, and the lower side of the lower arc-shaped piece is attached to the surface of the placing groove; a plurality of scraping pieces for scraping the fat tissue on the outer surface of the mouse intestine are arranged on each arc-shaped piece.

[0010] As preferred, the baffle is further included; the baffle is fixedly connected to the recess of the upper arc-shaped piece to prevent the fat tissue from being retained on the placing groove.

[0011] As preferred, the half ring and the push block are further included; the arc-shaped block is arranged on the upper side of the second clamping block; the rotating block is arranged on the lower side of the second clamping block; a half ring is fixedly connected to the arc-shaped block and the rotating block, and the two half rings are attached together to form a complete ring; grooves are arranged on the upper and lower sides of the second clamping block, and the half rings slide in the grooves; the push block is fixedly connected to the right side of the rotating block of the second clamping block to improve the cleaning effect of the outer surface of the mouse intestine.

[0012] As preferred, the curved arc pieces and the fixed block are further included; a plurality of curved arc pieces for extruding the contents of the mouse intestine are fixedly connected to the right side of the extruding rod, and each curved arc piece is made of a deformable material; a fixed block is fixedly connected to the right side of the plurality of curved arc pieces.

[0013] As preferred, the rolling balls are further included; a plurality of rolling balls are rotatably connected to the middle of each curved arc piece.

[0014] As preferred, the rubber pieces are further included; a rubber piece is fixedly connected between each adjacent two curved arc pieces.

[0015] The beneficial effects of the present application are as follows: the first clamping block and the second clamping block are used to fix the left end and the right end of the mouse intestine respectively, the injection tube is used to inject sterile water into the mouse intestine to flush, and the cleaning of the mouse intestine is completed, thereby reducing the pressure of manual operation and reducing the accidental damage of manual operation to the mouse intestine.

[0016] The scraping pieces on the two arc-shaped pieces pressed together are used to scrape the fat tissue on the outer surface of the mouse intestine, thereby preventing the existence of the fat tissue from affecting the accuracy of the sampling detection result.

[0017] The fat tissue after scraping is moved to the right along with the arc-shaped piece through the baffle, so that the fat tissue after scraping is prevented from being retained in the placing groove, the observation of the outer surface of the mouse intestine by the detection personnel is avoided from being affected, and the cleaning effect of the outer surface of the mouse intestine is improved;

[0018] The right end of the mouse intestine is driven to rotate through the push block, so that the scraping piece on the arc-shaped piece scrapes the fat tissue on each part of the outer surface of the mouse intestine, and the cleaning effect of the outer surface of the mouse intestine is further improved;

[0019] The contact area with the contents of the mouse intestine is increased through the arc-shaped piece, so that the discharge effect of the contents in the mouse intestine is improved, the contents adhered to the inner wall of the mouse intestine are scraped through the contact of the ball with the inner wall, and the cleaning effect of the inner wall of the mouse intestine is further improved, so that the cleaning is not affected, and the subsequent sampling detection result is affected. BRIEF DESCRIPTION OF DRAWINGS

[0020] Figure 1 It is a three-dimensional structure schematic view of the E. coli sampling and detection equipment for mouse experiments;

[0021] Figure 2 It is a sectional view of the sampling box;

[0022] Figure 3 It is a sectional view of the injection tube;

[0023] Figure 4 It is a three-dimensional structure schematic view of the combination of the fixing disc, the arc-shaped rod and the limiting ring;

[0024] Figure 5 It is a first three-dimensional structure schematic view of the scraping system;

[0025] Figure 6 It is a second three-dimensional structure schematic view of the scraping system;

[0026] Figure 7 It is a three-dimensional structure schematic view of the combination of the half ring and the push block;

[0027] Figure 8 It is a three-dimensional structure schematic view of the combination of the arc-shaped piece and the ball;

[0028] Figure 9 It is a three-dimensional structure schematic view of the combination of the arc-shaped piece, the ball and the rubber piece.

[0029] Explanation of reference numerals in the attached drawings: 1-Sampling box, 2-Placement block, 2001-Placement groove, 2002-Sliding groove, 3-First clamping block, 3001-Groove, 4-Second clamping block, 4001-Arc-shaped block, 4002-Rotating block, 5-Injection tube, 101-Fixing plate, 102-First fixing rod, 103-Second fixing rod, 104-Extrusion rod, 105-Fixing disc, 106-Arc-shaped rod, 107-Limiting ring, 201-Connecting block, 202-Arc-shaped piece, 202-201-Scraper, 203-Baffle, 301-Half ring, 302-Push block, 401-Bent arc piece, 402-Ball bearing, 403-Rubber sheet, 404-Fixing block. Detailed Implementation

[0030] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0031] Example 1

[0032] like Figures 2-4 As shown, a sampling and detection device for Escherichia coli in mouse experiments includes a sampling box 1;

[0033] It also includes a fixing component, a placement block 2, a first clamping block 3, a second clamping block 4, an injection tube 5, and a scraping system; the fixing component is installed inside the sampling box 1; the placement block 2 is set inside the sampling box 1; the placement block 2 is set with a placement groove 2001; the first clamping block 3 is slidably connected to the left side of the sampling box 1; the second clamping block 4 is slidably connected to the right side of the sampling box 1; the injection tube 5 is installed on the left side of the sampling box 1, and the injection tube 5 is set as a tapered tube placed horizontally to the right; the placement block 2 is set with a scraping system; by fixing the left and right ends of the mouse intestine with the first clamping block 3 and the second clamping block 4 respectively, the injection tube 5 injects sterile water into the mouse intestine for rinsing, thereby completing the cleaning of the mouse intestine, thereby reducing the pressure of manual operation and reducing accidental damage to the mouse intestine by manual operation.

[0034] The fixing assembly includes a fixing plate 101, a first fixing rod 102, and a second fixing rod 103. The fixing plate 101 is fixedly connected to the middle of the sampling box 1, and the lower side of the placement block 2 is fixedly connected to the fixing plate 101. The first fixing rod 102 is slidably connected to the left side of the sampling box 1, and the first clamping block 3 is fixedly connected to the first fixing rod 102. The second fixing rod 103 is slidably connected to the right side of the sampling box 1, and the second clamping block 4 is fixedly connected to the second fixing rod 103. The first clamping block 3 and the second clamping block 4 are controlled to move left and right by the first fixing rod 102 and the second fixing rod 103 respectively, so as to adjust their positions according to different intestinal lengths, which facilitates subsequent work.

[0035] The extrusion rod 104 is slidably connected to the left side of the sampling box 1 and located in the injection tube 5, and the contents in the mouse intestinal tract are pushed and penetrated by the extrusion rod 104, so that the flushing effect of the injection tube 5 on the inside of the mouse intestinal tract is improved.

[0036] The fixed disc 105, the arc-shaped rod 106 and the limiting ring 107 are further included; the fixed disc 105 is slidably connected to the right side of the extrusion rod 104; four circular rods are fixedly connected to the fixed disc 105; four arc-shaped rods 106 are fixedly connected to the fixed disc 105, and each arc-shaped rod 106 is made of a deformable material; the four arc-shaped rods 106 are located in the injection tube 5; the limiting ring 107 is fixedly connected to the right side of the extrusion rod 104 and located to the right of the fixed disc 105.

[0037] The mouse intestinal cleaning process is described in detail as follows: first, the entire mouse intestine after dissection is manually cut into sections according to requirements, then a section of mouse intestine is placed horizontally in the placement slot 2001 of the placement block 2, and according to the length of the mouse intestine, the first fixed rod 102 and the second fixed rod 103 are pushed respectively to make the left end and the right end of the mouse intestine located on the first clamp block 3 and the second clamp block 4 respectively, at this time the first clamp block 3 and the second clamp block 4 are in an open state, when the mouse intestine is placed, the first clamp block 3 and the second clamp block 4 are closed to fix the left end and the right end of the mouse intestine respectively, then according to the length and size of the mouse intestine, a suitable injection tube 5 is selected and aligned with the left opening of the mouse intestine, at this time the examiner slowly pushes the extrusion rod 104, at this time the fixed disc 105 and the arc-shaped rod 106 follow the extrusion rod 104 to move to the right until the fixed disc 105 moves to the injection tube 5, the circular rod on the fixed disc 105 is extruded by the injection tube 5 and cannot continue to move to the right, at the same time, the right end of the extrusion rod 104 and the arc-shaped rod 106 on the fixed disc 105 gradually move from the injection tube 5 to the inside of the left end of the mouse intestine, with the movement of the arc-shaped rod 106, the arc-shaped rod 106 gradually extrudes the injection tube 5 and gradually expands outward, thereby expanding the left end of the mouse intestine, at this time the sterile water is injected into the mouse intestine through the injection tube 5, and the part of the left end of the mouse intestine is clamped on the groove 3001 of the first clamp block 3 through the right end of the arc-shaped rod 106, thereby increasing the content of the sterile water injected into the mouse intestine through the injection tube 5, avoiding that most of the sterile water cannot flow smoothly from the left end of the mouse intestine to the right end due to the small opening, reducing resource waste, and preventing the mouse intestine from being separated from the first clamp block 3 due to the injection of sterile water by the injection tube 5, thereby affecting the cleaning efficiency of the mouse intestine, at the same time, the examiner continues to slowly push the extrusion rod 104 to the right according to the state of the mouse intestine, so that the extrusion rod 104 extrudes and penetrates the contents in the mouse intestine, thereby improving the flushing effect of the injection tube 5 on the inside of the mouse intestine, until the flushing is completed, according to requirements, the left and right ends of the mouse intestine after cleaning are removed, then the mouse intestine is cut into appropriate size tissue blocks and placed in a sterile cryogenic tube, then the examiner pulls the extrusion rod 104 back to the original position, at this time the limiting ring 107 gradually approaches the expanded arc-shaped rod 106 and extrudes the arc-shaped rod 106, so that the arc-shaped rod 106 moves to the left, at this time the arc-shaped rod 106 gradually changes from the expanded state to the folded state and gradually enters the injection tube 5, thereby facilitating the subsequent reuse of the device.

[0038] Example 2

[0039] Based on example 1, as Figure 5 and Figure 6As shown, the scraping system comprises a connecting block 201 and an arc-shaped piece 202; the placing block 2 is provided with two front and back symmetrical sliding grooves 2002, and a placing groove 2001 is located between the two sliding grooves 2002; the two sliding grooves 2002 are jointly and slidably connected with a connecting block 201; the connecting block 201 is movably connected with two arc-shaped pieces 202, and the two arc-shaped pieces 202 are rotatably connected; each arc-shaped piece 202 is provided in a downward convex arc shape, and the lower side of the lower arc-shaped piece 202 is attached to the surface of the placing groove 2001; a plurality of scrapers 20201 are arranged on each arc-shaped piece 202; the scrapers 20201 on the two arc-shaped pieces 202 pressed together scrape the fat tissue on the outer surface of the mouse intestine, so as to prevent the existence of the fat tissue from affecting the accuracy of the sampling detection result.

[0040] Further comprising a baffle 203; the upper arc-shaped piece 202 is fixedly connected with a baffle 203 in the recessed part; the baffle 203 makes the scraped fat tissue move to the right along with the arc-shaped piece 202, so as to prevent the scraped fat tissue from remaining in the placing groove 2001, avoid affecting the observation of the mouse intestine outer surface by the detection personnel, and improve the cleaning effect of the mouse intestine outer surface.

[0041] The cleaning of the mouse intestine outer surface is described in detail as follows: first, manually push the connecting block 201 to make the connecting block 201 located at the left end of the placing block 2, then place the mouse intestine cut into segments into the placing groove 2001, fix the left and right ends, then rotate the upper arc-shaped piece 202 to make it fold together with the lower arc-shaped piece 202, at this time the mouse intestine is located between the arc-shaped pieces 202, and since the two arc-shaped pieces 202 are both provided in a downward convex arc shape, the mouse intestine is in a closely attached state with the two arc-shaped pieces 202, according to the size of the mouse intestine, press the upper and lower arc-shaped pieces 202 together with appropriate force, then push the connecting block 201 to slide to the right in the sliding groove 2002, so that the scrapers 20201 on the arc-shaped pieces 202 pressed together scrape the fat tissue on the outer surface of the mouse intestine, so as to prevent the existence of the fat tissue from affecting the accuracy of the sampling detection result, and at the same time, in the process of moving the two arc-shaped pieces 202 to the right, the scraped fat tissue is blocked by the baffle 203, so as to prevent the scraped fat tissue from remaining in the placing groove 2001, affecting the observation of the mouse intestine outer surface by the detection personnel, and further improving the cleaning effect of the mouse intestine outer surface.

[0042] Example 3

[0043] On the basis of example 2, as Figure 7As shown, the second clamping block 4 further comprises a half ring 301 and a push block 302; the upper side of the second clamping block 4 is provided with an arc-shaped block 4001; the lower side of the second clamping block 4 is provided with a rotating block 4002; the arc-shaped block 4001 and the rotating block 4002 are both fixedly connected with a half ring 301, and the two half rings 301 are combined together to form a complete ring; the upper and lower sides of the second clamping block 4 are both provided with a groove, and the half ring 301 slides in the groove; the right side of the rotating block 4002 of the second clamping block 4 is fixedly connected with a push block 302; after the upper and lower two blocks of the second clamping block 4 are combined to fix the right end of the mouse, the tester pushes the push block 302 at the right end, and makes the push block 302 rotate around the groove on the second clamping block 4, so that the whole mouse intestine rotates, and at this time, sterile water flows through the inside of the mouse intestine, so that the mouse intestine is in a bulging state, thereby cooperating with the scraping piece 20201 on the arc-shaped piece 202 to scrape off the fat tissue on the surface of the mouse intestine, thereby further improving the cleaning effect of the surface of the mouse intestine.

[0044] Embodiment 4

[0045] Based on embodiments 1-3, as shown in Figure 1 、 Figure 8 and Figure 9 , the device further comprises a curved arc piece 401 and a fixed block 404; the right side of the extrusion rod 104 is fixedly connected with five curved arc pieces 401, and each curved arc piece 401 is made of a deformable material; the right side of the five curved arc pieces 401 is jointly fixedly connected with a fixed block 404; when the extrusion rod 104 extrudes the contents of the mouse intestine, the fixed block 404 is extruded and moves to the left, thereby extruding the curved arc piece 401 and making it deform, gradually protruding towards the inner wall of the mouse intestine, achieving the purpose of increasing the contact area with the contents of the mouse intestine, thereby improving the discharge effect of the contents inside the mouse intestine and improving the sampling efficiency.

[0046] The device further comprises a ball 402; four balls 402 are rotatably connected to the middle of each curved arc piece 401; after the curved arc piece 401 deforms, the balls 402 come into contact with the inner wall of the mouse intestine and scrape off the contents adhering to the inner wall, further improving the cleaning effect of the inner wall of the mouse intestine and avoiding incomplete cleaning, which affects the subsequent sampling and detection results.

[0047] The device further comprises a rubber piece 403; one rubber piece 403 is fixedly connected between each adjacent two curved arc pieces 401; the deformed curved arc pieces 401 are still in a closed state through the rubber piece 403, preventing the curved arc piece 401 from having gaps after deformation, which may scratch the inner wall of the mouse intestine during scraping and affect the subsequent sampling and detection results.

[0048] The embodiments of the present application are described in detail above with reference to the accompanying drawings, but the present application is not limited to the above-described embodiments, and various changes can be made within the knowledge of those skilled in the art without departing from the spirit of the present application.

Claims

1. A sampling and detection device for Escherichia coli in mouse experiments, comprising a sampling box (1); characterized in that, It also includes a fixing component, a placement block (2), a first clamping block (3), a second clamping block (4), an injection tube (5), and a scraping system; the fixing component is installed inside the sampling box (1); the placement block (2) for placing mouse intestines is provided inside the sampling box (1); a placement groove (2001) is provided on the placement block (2); the left side of the sampling box (1) is slidably connected to a first clamping block (3) for fixing the left end of the mouse intestines; the right side of the sampling box (1) is slidably connected to a second clamping block (4) for fixing the right end of the mouse intestines; an injection tube (5) for rinsing the inside of the mouse intestines is installed on the left side of the sampling box (1), and the injection tube (5) is set as a tapered tube placed horizontally to the right; a scraping system for scraping off the adipose tissue on the outer surface of the mouse intestines is provided on the placement block (2); It also includes a squeezing rod (104); the left side of the sampling box (1) is slidably connected to a squeezing rod (104) for squeezing the contents inside the mouse intestine, and the squeezing rod (104) is located inside the injection tube (5); It also includes a fixed plate (105), an arc rod (106) and a limiting ring (107); the fixed plate (105) is slidably connected to the right side of the extrusion rod (104); a number of round rods for limiting the movement of the fixed plate (105) are fixedly connected to the fixed plate (105); a number of arc rods (106) are fixedly connected to the fixed plate (105), and each arc rod (106) is made of deformable material; the number of arc rods (106) are all located inside the injection tube (5); a limiting ring (107) is fixedly connected to the right side of the extrusion rod (104), and the limiting ring (107) is located to the right of the fixed plate (105).

2. The Escherichia coli sampling and detection device for mouse experiments according to claim 1, characterized in that, The fixing assembly includes a fixing plate (101), a first fixing rod (102), and a second fixing rod (103); the fixing plate (101) is fixedly connected to the middle of the sampling box (1), and the lower side of the placement block (2) is fixedly connected to the fixing plate (101); the first fixing rod (102) is slidably connected to the left side of the sampling box (1), and the first clamping block (3) is fixedly connected to the first fixing rod (102); the second fixing rod (103) is slidably connected to the right side of the sampling box (1), and the second clamping block (4) is fixedly connected to the second fixing rod (103).

3. The Escherichia coli sampling and detection device for mouse experiments according to claim 1, characterized in that, The scraping system includes a connecting block (201) and an arc-shaped piece (202); the placement block (2) is provided with several sliding grooves (2002), and the placement groove (2001) is located between several sliding grooves (2002); a connecting block (201) is slidably connected between several sliding grooves (2002); two arc-shaped pieces (202) are movably connected on the connecting block (201), and the two arc-shaped pieces (202) are rotatably connected, and the upper arc-shaped piece (202) is rotated to make it close together with the lower arc-shaped piece (202); each arc-shaped piece (202) is set as a downward convex curved arc, and the lower side of the lower arc-shaped piece (202) is in contact with the surface of the placement groove (2001); each arc-shaped piece (202) is provided with several scraping blades (20201) for scraping the adipose tissue on the outer surface of the mouse intestine.

4. The Escherichia coli sampling and detection device for mouse experiments according to claim 3, characterized in that, It also includes a baffle (203); the upper arc-shaped plate (202) has a baffle (203) fixed to the recess to prevent adipose tissue from being retained on the placement slot (2001).

5. The Escherichia coli sampling and detection device for mouse experiments according to claim 1, characterized in that, It also includes a semi-ring (301) and a push block (302); an arc-shaped block (4001) is provided on the upper side of the second clamping block (4); a rotating block (4002) is provided on the lower side of the second clamping block (4); a semi-ring (301) is fixedly connected to both the arc-shaped block (4001) and the rotating block (4002), and the two semi-rings (301) are fitted together to form a complete ring; grooves are provided on both the upper and lower sides of the second clamping block (4), and the semi-ring (301) slides in the groove; a push block (302) is fixedly connected to the right side of the rotating block (4002) of the second clamping block (4), and the push block (302) rotates around the groove on the second clamping block (4).

6. The Escherichia coli sampling and detection device for mouse experiments according to claim 1, characterized in that, It also includes curved plates (401) and fixing blocks (404); several curved plates (401) are fixed to the right side of the squeezing rod (104), which protrude towards the inner wall of the mouse intestine after being squeezed by the contents of the mouse intestine, and each curved plate (401) is made of deformable material; a fixing block (404) is fixed to the right side of several curved plates (401).

7. The Escherichia coli sampling and detection device for mouse experiments according to claim 6, characterized in that, It also includes ball bearings (402); each curved plate (401) has several ball bearings (402) rotatably connected in the middle.

8. The Escherichia coli sampling and detection device for mouse experiments according to claim 6, characterized in that, It also includes a rubber sheet (403); a rubber sheet (403) is fixed between each two adjacent curved sheets (401).

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